CN102318559A - Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method - Google Patents
Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method Download PDFInfo
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- CN102318559A CN102318559A CN201110238282A CN201110238282A CN102318559A CN 102318559 A CN102318559 A CN 102318559A CN 201110238282 A CN201110238282 A CN 201110238282A CN 201110238282 A CN201110238282 A CN 201110238282A CN 102318559 A CN102318559 A CN 102318559A
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Abstract
The invention relates to the technical field of plant tissue culture, and particularly relates to a culture medium composition for rapid rooting of blueberry tissue culture seedlings outside a test tube and a preparation method. The composition comprises: a basal culture medium of WPM culture medium, wherein 20 g of white sugar is added into each liter of WPM culture medium; a rooting culture solution, wherein a culture medium diluent is obtained by diluting the basal culture medium by 6 times, and 25 mg of auxin is added into each liter of the culture medium diluent; a culture medium supporting substance of perlite, wherein the ratio of perlite and the rooting culture solution is 375 g/L. The culture medium composition for rapid rooting of blueberry tissue culture seedlings outside a test tube and the preparation method of the invention solves the problems of low rooting rate of blueberry tissue culture seedlings outside bottles (outside test tubes), unstable rooting condition, and long rooting time.
Description
Technical field
The present invention relates to field of plant tissue culture technique, is culture media composition and preparation method that blueberry tissue cultivating seedling test tube is taken root outward fast specifically.
Background technology
It is anticancer, anti-oxidant and recover health-care effect such as eyesight that blueberry has, and is called third generation fruit in the world, classified as one of five big healthy fruit by international food and agricultural organization.Extremely sell well in blueberry market, everybody is with the fashion of consumption blueberry as health care in recent years.
We investigate discovery, and domestic blueberry industry belongs to the fruit of high investment, and seedling cost becomes a cost that accounts for significant proportion.Therefore, reduce blueberry nursery stock price, let more common plantation family also can cultivate blueberry, reduce the risk that they develop the blueberry plantation, the dining table that lets blueberry move towards ordinary people becomes a vital task of present blueberry industry development.
In recent years, R&D institution in all parts of the country has carried out the work of blueberry group cultivation seedling.Blueberry tissue cultivating seedling reproductive speed is fast, can promote new quality product kind rapidly.Become the means of mainly growing seedlings of blueberry seedling.
But the specification requirement of blueberry tissue cultivating seedling is high, the difficulty of particularly taking root in the tissue cultivating seedling bottle, at present 45 days rooting rates only about 60～80% in the bottle seedling bottle; And the root system of taking root in the bottle is of poor quality; Owing to be stained with medium, be not properly cleaned most medium during cleaning, transplant the assorted bacterium breeding in back and cause survival rate low.Under the effort of other R&D institutions, part has solved blueberry tissue cultivating seedling outside sprout-cultivating-bottle technology.But still it is low to show as rooting rate, the situation of taking root instability, and rootage duration is longer.
Summary of the invention
To the defective that exists in the prior art; The object of the present invention is to provide the culture media composition that blueberry tissue cultivating seedling test tube takes root fast, the preparation method of culture media composition outward; And the blueberry tissue cultivating seedling test tube tissue culture method of taking root fast outward; It is low to solve blueberry tissue cultivating seedling bottle outer (test tube is outer) rooting rate, the situation of taking root instability, the problem that rootage duration is long.
For reaching above purpose, the technical scheme that the present invention takes is:
The culture media composition that blueberry tissue cultivating seedling test tube is taken root outward fast is characterized in that, its component comprises:
Minimal medium: the WPM medium, add white sugar 20g in every liter of WPM medium;
Culture of rootage liquid: the minimal medium dilution is obtained the medium dilution for 6 times; In every liter of medium dilution, add growth hormone 25mg;
Culture medium supports: perlite, the ratio of perlite and culture of rootage liquid is 375g/L.
On the basis of technique scheme, said growth hormone is IBA or NAA.
The preparation method of the culture media composition that blueberry tissue cultivating seedling test tube is taken root outward fast is characterized in that, the preparation process is following:
Step 1, preparation minimal medium: according to the formulated WPM medium of WPM medium, in every liter of WPM medium, behind the interpolation white sugar 20g, make minimal medium then earlier;
Step 2; Preparation culture of rootage liquid: minimal medium is obtained the medium dilution for 6 times with the running water dilution; In every liter of medium dilution, add growth hormone 25mg again, after mixing, regulate pH value to 4.5～5.2 of mixture; The mixture that to reconcile the pH value again is heated to boiling, and cooling promptly makes culture of rootage liquid;
Step 3, the perlite of selecting pure free from admixture to use as horticultural gardening matrix, particle diameter are 3～7mm, add perlite 375g by every liter of culture of rootage liquid and take by weighing an amount of perlite;
Step 4 after perlite and culture of rootage liquid is mixed, is put into container, promptly accomplishes the preparation of culture media composition.
On the basis of technique scheme, the size of said container is the plastic casing of 40 * 20 * 10cm.
On the basis of technique scheme, in perlite and culture of rootage liquid are mixed process, require the blunger to be with the gloves operation, stir not water breakthrough of back container bottom, there is not dry especially matrix.
On the basis of technique scheme, the amount that perlite and culture of rootage liquid are put into container is the half the of container volume.
The cultural method that blueberry tissue cultivating seedling test tube is taken root outward fast is characterized in that, its concrete steps are:
Step 1; With robust growth, the blueberry tissue cultivating seedling that does not have to pollute directly takes out in tissue culture bottle, cuts off the lower end that is stained with medium with scissors; Clamp the lower end of tissue cultivating seedling with tweezers; Cuttage is puddled in container in the pearlite interstitial substance of culture of rootage liquid, and the cuttage degree of depth is 1-2cm, and every box cuttage seeding amount is 190～210;
Step 2 is wrapped up one deck with the container of the good seedling of cuttage with preservative film, places 25 ± 2 ℃; Cultivated for 3 weeks under the illumination condition 3000-1000lux condition, place again under the outdoor 23-30 ℃ condition, tear preservative film; Refined seedling 3-7 days, taking root to tissue cultivating seedling reaches 3～7 centimetres;
Step 3; Growth reached 3～7 centimetres the tissue cultivating seedling of taking root; Need not wash down root medium direct transplanting to the cultivation matrix of being puddled by garden mould, peat, perlite, rice chaff, the part by weight that said garden mould, peat, perlite, rice chaff are puddled is 3: 1: 0.5: 0.5.
Culture media composition that blueberry tissue cultivating seedling test tube of the present invention is taken root outward fast and preparation method (the blueberry tissue cultivating seedling test tube culture media composition of taking root fast outward, the preparation method of culture media composition; And the blueberry tissue cultivating seedling test tube tissue culture method of taking root fast outward); It is low to have solved blueberry tissue cultivating seedling bottle outer (test tube is outer) rooting rate; The problem that the situation of taking root instability, rootage duration are long, its advantage is:
(1) the culture of rootage formula of liquid and the composition thereof that propose obviously improved rooting rate and rootage duration, and the root system quality are good, and the radical amount is many.
(2) culture medium supports that adopts is a perlite, and light weight is cheap, is prone to obtain.And nursery stock adaptability is good when transplanting, and transplanting survival rate is more than 95%.
(3) utilize running water to replace distilled water, white sugar place of sucrose, thus saving group training cost.
(4) use the plastics square box instead and hold matrix, be suitable for very much the dense planting of blueberry tissue cultivating seedling, every box cuttage seeding amount can reach more than 250.1 cubic metre of perlite of every consumption can be produced blueberry and take root seedling more than 100,000, efficiently utilizes matrix and medium.
(5) convenient cultivation, mobile and carrying, this kind method can not receive the restriction of outdoor temperature in the indoor stereoscopic culture of carrying out.When more helping factorial seedling growth stage by stage, the branch place carries out.
Embodiment
English abbreviation and abbreviation among this paper:
WPM:woody plant medium, the woody plant medium;
IBA: indolebutyric acid;
NAA: methyl;
G/L: restrain every liter;
Mg/L: every liter of milligram;
PH: acid-base value; Hydrogen ion concentration;
Mm: millimeter;
Cm: centimetre;
G: gram;
Lux: illuminance unit.
The culture media composition that blueberry tissue cultivating seedling test tube of the present invention is taken root outward fast, its component comprises:
Minimal medium: the see after formula table 1 of face of WPM medium (Lloyd&McCown 1980, and these two people of Lloyd and McCown are at this prescription of invention in 1980), the prescription of said WPM medium; In every liter of WPM medium, add white sugar 20g; The ratio that is white sugar and WPM medium is 20g/L;
Culture of rootage liquid: the minimal medium dilution is obtained the medium dilution for 6 times; In every liter of medium dilution, add growth hormone 25mg, said growth hormone is IBA or NAA; The ratio that is growth hormone and medium dilution is 25mg/L;
Culture medium supports: perlite, the ratio of perlite and culture of rootage liquid is 375g/L, promptly adds perlite 375g in every liter of culture of rootage liquid.
The culture media composition that blueberry tissue cultivating seedling test tube of the present invention is taken root outward fast, its preparation process is following:
Step 1, preparation minimal medium: according to the formulated WPM medium of WPM medium, in every liter of WPM medium, behind the interpolation white sugar 20g, make minimal medium then earlier;
Step 2; Preparation culture of rootage liquid: minimal medium is obtained the medium dilution for 6 times with the running water dilution; In every liter of medium dilution, add growth hormone 25mg again, after mixing, regulate pH value to 4.5～5.2 of mixture; The mixture that to reconcile the pH value again is heated to boiling, and cooling promptly makes culture of rootage liquid;
Step 3, the perlite of selecting pure free from admixture to use as horticultural gardening matrix, particle diameter are 3～7mm, add perlite 375g by every liter of culture of rootage liquid and take by weighing an amount of perlite;
Step 4 after perlite and culture of rootage liquid is mixed, is put into container, promptly accomplishes the preparation of culture media composition.
The size of said container is 40 * 20 * 10cm.Container can be a plastic casing.Efficient is low very little for the too small cuttage seeding number of container, and how inconvenience has been crossed seedlings and still caused pollution easily, and therefore through experiment, optimum is 40 * 20 * 10cm.
On the basis of technique scheme, in perlite and culture of rootage liquid are mixed process, require the blunger to be with the gloves operation, stir not water breakthrough of back container bottom, there is not dry especially matrix.
On the basis of technique scheme, the amount that perlite and culture of rootage liquid are put into container is the half the of container volume.
The present invention gives with above-mentioned culture media composition and carries out the cultural method that blueberry tissue cultivating seedling test tube is taken root fast outward, and its concrete steps are:
Step 1; With robust growth, the blueberry tissue cultivating seedling that does not have to pollute directly takes out in tissue culture bottle, cuts off the lower end that is stained with medium with scissors; Clamp the lower end of tissue cultivating seedling with tweezers; Cuttage is puddled in container in the pearlite interstitial substance of culture of rootage liquid, and the cuttage degree of depth is 1-2cm, and every box cuttage seeding amount is 190～210; Be advisable 200 the bests for 195～205;
Step 2 is wrapped up one deck with the container of the good seedling of cuttage with preservative film, places 25 ± 2 ℃; Cultivated for 3 weeks under the illumination condition 3000-1000lux condition, place again under the outdoor 23-30 ℃ condition, tear preservative film; Refined seedling 3-7 days, taking root to tissue cultivating seedling reaches 3～7 centimetres;
Step 3; Growth reached 3～7 centimetres the tissue cultivating seedling of taking root; Need not wash down root medium direct transplanting to the cultivation matrix of being puddled by garden mould, peat, perlite, rice chaff, the part by weight that said garden mould, peat, perlite, rice chaff are puddled is 3: 1: 0.5: 0.5.
The content of not doing in this specification to describe in detail belongs to this area professional and technical personnel's known prior art.
WPM culture medium prescription table 1
Claims (7)
1. the blueberry tissue cultivating seedling test tube culture media composition of taking root fast outward is characterized in that its component comprises:
Minimal medium: the WPM medium, add white sugar 20g in every liter of WPM medium;
Culture of rootage liquid: the minimal medium dilution is obtained the medium dilution for 6 times; In every liter of medium dilution, add growth hormone 25mg;
Culture medium supports: perlite, the ratio of perlite and culture of rootage liquid is 375g/L.
2. the culture media composition that blueberry tissue cultivating seedling test tube as claimed in claim 1 is taken root outward fast is characterized in that: said growth hormone is IBA or NAA.
3. the preparation method of the said blueberry tissue cultivating seedling of the claim 1 test tube culture media composition of taking root fast outward is characterized in that the preparation process is following:
Step 1, preparation minimal medium: according to the formulated WPM medium of WPM medium, in every liter of WPM medium, behind the interpolation white sugar 20g, make minimal medium then earlier;
Step 2; Preparation culture of rootage liquid: minimal medium is obtained the medium dilution for 6 times with the running water dilution; In every liter of medium dilution, add growth hormone 25mg again, after mixing, regulate pH value to 4.5～5.2 of mixture; The mixture that to reconcile the pH value again is heated to boiling, and cooling promptly makes culture of rootage liquid;
Step 3, the perlite of selecting pure free from admixture to use as horticultural gardening matrix, particle diameter are 3～7mm, add perlite 375g by every liter of culture of rootage liquid and take by weighing an amount of perlite;
Step 4 after perlite and culture of rootage liquid is mixed, is put into container, promptly accomplishes the preparation of culture media composition.
4. the preparation method of the culture media composition that blueberry tissue cultivating seedling test tube as claimed in claim 3 is taken root outward fast is characterized in that: the size of said container is the plastic casing of 40 * 20 * 10cm.
5. the preparation method of the culture media composition that blueberry tissue cultivating seedling test tube as claimed in claim 3 is taken root outward fast; It is characterized in that: in perlite and culture of rootage liquid are mixed process; Require the blunger to be with the gloves operation, stir not water breakthrough of back container bottom, do not have dry especially matrix.
6. the preparation method of the culture media composition that blueberry tissue cultivating seedling test tube as claimed in claim 3 is taken root outward fast is characterized in that: the amount that perlite and culture of rootage liquid are put into container is the half the of container volume.
7. the preparation method of the culture media composition that blueberry tissue cultivating seedling test tube as claimed in claim 3 is taken root outward fast is characterized in that, also comprises following incubation step:
Step 1; With robust growth, the blueberry tissue cultivating seedling that does not have to pollute directly takes out in tissue culture bottle, cuts off the lower end that is stained with medium with scissors; Clamp the lower end of tissue cultivating seedling with tweezers; Cuttage is puddled in container in the pearlite interstitial substance of culture of rootage liquid, and the cuttage degree of depth is 1-2cm, and every box cuttage seeding amount is 190～210;
Step 2 is wrapped up one deck with the container of the good seedling of cuttage with preservative film, places 25 ± 2 ℃; Cultivated for 3 weeks under the illumination condition 3000-1000lux condition, place again under the outdoor 23-30 ℃ condition, tear preservative film; Refined seedling 3-7 days, taking root to tissue cultivating seedling reaches 3～7 centimetres;
Step 3; Growth reached 3～7 centimetres the tissue cultivating seedling of taking root; Need not wash down root medium direct transplanting to the cultivation matrix of being puddled by garden mould, peat, perlite, rice chaff, the part by weight that said garden mould, peat, perlite, rice chaff are puddled is 3: 1: 0.5: 0.5.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823499A (en) * | 2012-09-20 | 2012-12-19 | 重庆文理学院 | Factorized breeding method of blueberry tissue-cultured seedlings |
| CN103798096A (en) * | 2014-03-12 | 2014-05-21 | 金陵科技学院 | Method for improving blueberry sapling planting quality |
| CN103875515A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Ex-vitro rooting method for tissue culture blueberry seedlings |
| CN104170730A (en) * | 2013-05-25 | 2014-12-03 | 贵州瑞蓝果业科技发展有限责任公司 | Rooting cultivation technology for blueberry microbody virus-free seedling |
| CN106171989A (en) * | 2016-07-13 | 2016-12-07 | 吉林省普蓝高科技有限公司 | Alleviate culture medium of blueberry tissue culture Seedling brownization and preparation method thereof |
| CN106577094A (en) * | 2016-10-27 | 2017-04-26 | 安徽紫约农业科技有限公司 | Blueberry whole-matrix cultivation method |
| CN106613962A (en) * | 2016-11-14 | 2017-05-10 | 宁波市农业科学研究院 | Culture medium for rooting of tissue-cultured blueberry seedling and culturing method using same |
| CN108770691A (en) * | 2018-05-21 | 2018-11-09 | 西南林业大学 | A method of induction camphor tree leaf blueberry tissue culture seedling leaf directly generates adventitious root |
| CN112167034A (en) * | 2020-08-07 | 2021-01-05 | 兰婷 | Method for quickly breeding blueberries and natural light microcirculation cultivation box |
| CN114946625A (en) * | 2022-06-09 | 2022-08-30 | 安徽科技学院 | Method for rooting outside test tube of pomegranate tissue culture seedling |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823499A (en) * | 2012-09-20 | 2012-12-19 | 重庆文理学院 | Factorized breeding method of blueberry tissue-cultured seedlings |
| CN104170730A (en) * | 2013-05-25 | 2014-12-03 | 贵州瑞蓝果业科技发展有限责任公司 | Rooting cultivation technology for blueberry microbody virus-free seedling |
| CN103875515A (en) * | 2014-02-18 | 2014-06-25 | 杭州佑国生物科技有限公司 | Ex-vitro rooting method for tissue culture blueberry seedlings |
| CN103875515B (en) * | 2014-02-18 | 2017-01-04 | 杭州佑国生物科技有限公司 | A kind of blueberry tissue culture outside sprout-cultivating-bottle radication method |
| CN103798096A (en) * | 2014-03-12 | 2014-05-21 | 金陵科技学院 | Method for improving blueberry sapling planting quality |
| CN106171989A (en) * | 2016-07-13 | 2016-12-07 | 吉林省普蓝高科技有限公司 | Alleviate culture medium of blueberry tissue culture Seedling brownization and preparation method thereof |
| CN106577094A (en) * | 2016-10-27 | 2017-04-26 | 安徽紫约农业科技有限公司 | Blueberry whole-matrix cultivation method |
| CN106613962A (en) * | 2016-11-14 | 2017-05-10 | 宁波市农业科学研究院 | Culture medium for rooting of tissue-cultured blueberry seedling and culturing method using same |
| CN108770691A (en) * | 2018-05-21 | 2018-11-09 | 西南林业大学 | A method of induction camphor tree leaf blueberry tissue culture seedling leaf directly generates adventitious root |
| CN112167034A (en) * | 2020-08-07 | 2021-01-05 | 兰婷 | Method for quickly breeding blueberries and natural light microcirculation cultivation box |
| CN114946625A (en) * | 2022-06-09 | 2022-08-30 | 安徽科技学院 | Method for rooting outside test tube of pomegranate tissue culture seedling |
| CN114946625B (en) * | 2022-06-09 | 2023-05-26 | 安徽科技学院 | Method for rooting pomegranate tissue culture seedling outside test tube |
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Application publication date: 20120118 |