CN104165919A - Method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection - Google Patents
Method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection Download PDFInfo
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- CN104165919A CN104165919A CN201410434317.6A CN201410434317A CN104165919A CN 104165919 A CN104165919 A CN 104165919A CN 201410434317 A CN201410434317 A CN 201410434317A CN 104165919 A CN104165919 A CN 104165919A
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Abstract
The invention discloses a method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection. According to the method, a capillary electrophoresis method is used, and hydrochlorothiazide is taken as an interior label for quantitatively determining cefoperazone sodium and tazobactam sodium. The method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection is relatively simple and quick, low in cost and capable of effectively controlling quality in the process of producing cefoperazone sodium and tazobactam sodium preparations for injection.
Description
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Technical field
The present invention relates to medicine bioengineering field, particularly, relate to the method for measuring active constituent content in Cefoperazone Sodium and Tazobactam.
Background technology
Cefoperazone is Third generation Cephalosporins microbiotic, by suppressing the biosynthesizing of sensitive bacterial cell membrane, reaches bactericidal action.Tazobactam Sodium is except to Neisseriaceae and acinetobacter calcoaceticus, to other bacteriums without antibacterial activity, but Tazobactam Sodium has the inhibiting effect of nonreversibility to the important beta-lactamase of majority being produced by β-lactam antibiotics antibody-resistant bacterium.Tazobactam Sodium can prevent the destruction of drug-fast bacteria to penicillins and cephalosporins, and Tazobactam Sodium and penicillins and cephalosporins have obvious synergy.Because Tazobactam Sodium can combine with some PBP, so sensitive strain is stronger may be to the more alone cefoperazone of susceptibility of this compound preparation time.
In existing mensuration Cefoperazone Sodium and Tazobactam, the method for object content is high performance liquid chromatography, but this method bothers, cost is higher.
Summary of the invention
Technical matters to be solved by this invention is to provide the method for measuring active constituent content in Cefoperazone Sodium and Tazobactam, the method adopts capillary electrophoresis, take Hydrochioro as interior mark, cefoperazone sodium and sodium-tazobactam are carried out to quantitative measurement simultaneously, a kind of more simple and efficient, detection method that cost is low is provided, in Cefoperazone Sodium and Tazobactam preparation production run, can effectively carry out quality control.
The present invention addresses the above problem adopted technical scheme:
The method of measuring active constituent content in Cefoperazone Sodium and Tazobactam, comprises the following steps:
(1) mark liquid, Cefoperazone Sodium and Tazobactam liquid to be measured, cefoperazone sodium reference substance solution, sodium-tazobactam reference substance solution in preparation, described interior mark liquid is Hydrochioro solution;
(2) taking 69mg Cefoperazone Sodium and Tazobactam is dissolved in volumetric flask, be placed in 100mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale, shake up rear absorption 9mL, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up, send sample introduction in efficient capillary electrophoresis apparatus, carry out determination and analysis, with relative peak area, press the content that calibration curve method calculates cefoperazone sodium and sodium-tazobactam.
Being formulated as of described interior mark liquid takes Hydrochioro 5.0mg, is placed in 10mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale, shakes up and get final product.
Described sample injection time is 8-15s.
The separation voltage of described efficient capillary electrophoresis apparatus is 12kV.
The Cefoperazone Sodium and Tazobactam that being formulated as of described Cefoperazone Sodium and Tazobactam liquid to be measured takes 69mg is placed in 100mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale and shake up, the accurate 9mL that draws, be placed in 10mL volumetric flask, add 1.0mL inner mark solution to shake up and get final product.
Being formulated as of described cefoperazone sodium reference substance solution takes 55mg cefoperazone sodium and is placed in 100mL volumetric flask with methyl alcohol ultrasonic dissolution and is settled to scale and shake up, from 100mL volumetric flask, draw 9mL again, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up and get final product.
Being formulated as of described sodium-tazobactam reference substance solution takes 14mg sodium-tazobactam and is placed in 100mL volumetric flask with methyl alcohol ultrasonic dissolution and is settled to scale and shake up, from 100mL volumetric flask, draw 9mL again, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up and get final product.
To sum up, the invention has the beneficial effects as follows:
The present invention adopts capillary electrophoresis, take Hydrochioro as interior mark, cefoperazone sodium and sodium-tazobactam are carried out to quantitative measurement simultaneously, a kind of more simple and efficient, detection method that cost is low is provided, in Cefoperazone Sodium and Tazobactam preparation production run, can effectively carry out quality control.
Embodiment
Below in conjunction with embodiment, the present invention is done to detailed description further, but embodiments of the present invention are not limited to this.
Embodiment 1:
The method of measuring active constituent content in Cefoperazone Sodium and Tazobactam, comprises the following steps:
(1) mark liquid, Cefoperazone Sodium and Tazobactam liquid to be measured, cefoperazone sodium reference substance solution, sodium-tazobactam reference substance solution in preparation, described interior mark liquid is Hydrochioro solution;
(2) taking 69mg Cefoperazone Sodium and Tazobactam is dissolved in volumetric flask, be placed in 100mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale, shake up rear absorption 9mL, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up, send sample introduction in efficient capillary electrophoresis apparatus, carry out determination and analysis, with relative peak area, press the content that calibration curve method calculates cefoperazone sodium and sodium-tazobactam.
Being formulated as of described interior mark liquid takes Hydrochioro 5.0mg, is placed in 10mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale, shakes up and get final product.
Described sample injection time is 8-15s.
The separation voltage of described efficient capillary electrophoresis apparatus is 12kV.
The Cefoperazone Sodium and Tazobactam that being formulated as of described Cefoperazone Sodium and Tazobactam liquid to be measured takes 69mg is placed in 100mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale and shake up, the accurate 9mL that draws, be placed in 10mL volumetric flask, add 1.0mL inner mark solution to shake up and get final product.
Being formulated as of described cefoperazone sodium reference substance solution takes 55mg cefoperazone sodium and is placed in 100mL volumetric flask with methyl alcohol ultrasonic dissolution and is settled to scale and shake up, from 100mL volumetric flask, draw 9mL again, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up and get final product.
Being formulated as of described sodium-tazobactam reference substance solution takes 14mg sodium-tazobactam and is placed in 100mL volumetric flask with methyl alcohol ultrasonic dissolution and is settled to scale and shake up, from 100mL volumetric flask, draw 9mL again, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up and get final product.
Claims (7)
1. measure the method for active constituent content in Cefoperazone Sodium and Tazobactam, it is characterized in that, comprise the following steps:
(1) mark liquid, Cefoperazone Sodium and Tazobactam liquid to be measured, cefoperazone sodium reference substance solution, sodium-tazobactam reference substance solution in preparation, described interior mark liquid is Hydrochioro solution;
(2) taking 69mg Cefoperazone Sodium and Tazobactam is dissolved in volumetric flask, be placed in 100mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale, shake up rear absorption 9mL, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up, send sample introduction in efficient capillary electrophoresis apparatus, carry out determination and analysis, with relative peak area, press the content that calibration curve method calculates cefoperazone sodium and sodium-tazobactam.
2. the method for active constituent content in mensuration Cefoperazone Sodium and Tazobactam according to claim 1, it is characterized in that, being formulated as of described interior mark liquid takes Hydrochioro 5.0mg, is placed in 10mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale, shake up and get final product.
3. the method for active constituent content in mensuration Cefoperazone Sodium and Tazobactam according to claim 1, is characterized in that, described sample injection time is 8-15s.
4. the method for active constituent content in mensuration Cefoperazone Sodium and Tazobactam according to claim 1, is characterized in that, the separation voltage of described efficient capillary electrophoresis apparatus is 12kV.
5. the method for active constituent content in mensuration Cefoperazone Sodium and Tazobactam according to claim 1, it is characterized in that, the Cefoperazone Sodium and Tazobactam that being formulated as of described Cefoperazone Sodium and Tazobactam liquid to be measured takes 69mg is placed in 100mL volumetric flask, with methyl alcohol ultrasonic dissolution and be settled to scale and shake up, the accurate 9mL that draws, be placed in 10mL volumetric flask, add 1.0mL inner mark solution to shake up and get final product.
6. the method for active constituent content in mensuration Cefoperazone Sodium and Tazobactam according to claim 1, it is characterized in that, being formulated as of described cefoperazone sodium reference substance solution takes 55mg cefoperazone sodium and is placed in 100mL volumetric flask with methyl alcohol ultrasonic dissolution and is settled to scale and shake up, from 100mL volumetric flask, draw 9mL again, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up and get final product.
7. the method for active constituent content in mensuration Cefoperazone Sodium and Tazobactam according to claim 1, it is characterized in that, being formulated as of described sodium-tazobactam reference substance solution takes 14mg sodium-tazobactam and is placed in 100mL volumetric flask with methyl alcohol ultrasonic dissolution and is settled to scale and shake up, from 100mL volumetric flask, draw 9mL again, be placed in 10mL volumetric flask, add 1mL inner mark solution to shake up and get final product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410434317.6A CN104165919A (en) | 2014-08-29 | 2014-08-29 | Method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection |
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| CN201410434317.6A CN104165919A (en) | 2014-08-29 | 2014-08-29 | Method for determining contents of effective components in cefoperazone sodium and tazobactam sodium for injection |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1971258A (en) * | 2005-11-22 | 2007-05-30 | 中国药品生物制品检定所 | Method for evaluating stability of cefoperazone sodium leechdom |
| US20120245128A1 (en) * | 2011-03-25 | 2012-09-27 | The Board Of Regents Of The University Of Texas System | Rapid detection and quantification of modification of medicinal compounds and drug resistance activity |
| WO2012167218A2 (en) * | 2011-06-03 | 2012-12-06 | Dxupclose | Device and method for identifying microbes and counting microbes and determining antimicrobial sensitivity |
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2014
- 2014-08-29 CN CN201410434317.6A patent/CN104165919A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1971258A (en) * | 2005-11-22 | 2007-05-30 | 中国药品生物制品检定所 | Method for evaluating stability of cefoperazone sodium leechdom |
| US20120245128A1 (en) * | 2011-03-25 | 2012-09-27 | The Board Of Regents Of The University Of Texas System | Rapid detection and quantification of modification of medicinal compounds and drug resistance activity |
| WO2012167218A2 (en) * | 2011-06-03 | 2012-12-06 | Dxupclose | Device and method for identifying microbes and counting microbes and determining antimicrobial sensitivity |
Non-Patent Citations (1)
| Title |
|---|
| 姜睿远 等: "毛细管电泳法测定注射用头孢哌酮钠他唑巴坦钠中目标成分", 《色谱》, vol. 30, no. 1, 31 January 2012 (2012-01-31) * |
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Application publication date: 20141126 |