IN2015DN02992A - - Google Patents
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- Publication number
- IN2015DN02992A IN2015DN02992A IN2992DEN2015A IN2015DN02992A IN 2015DN02992 A IN2015DN02992 A IN 2015DN02992A IN 2992DEN2015 A IN2992DEN2015 A IN 2992DEN2015A IN 2015DN02992 A IN2015DN02992 A IN 2015DN02992A
- Authority
- IN
- India
- Prior art keywords
- sample
- measured
- calibration curve
- quantification
- sodium
- Prior art date
Links
- 239000000523 sample Substances 0.000 abstract 6
- 238000000034 method Methods 0.000 abstract 5
- 238000011088 calibration curve Methods 0.000 abstract 4
- 238000005259 measurement Methods 0.000 abstract 4
- 238000011002 quantification Methods 0.000 abstract 4
- 229910001415 sodium ion Inorganic materials 0.000 abstract 3
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 abstract 2
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 abstract 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 abstract 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- 241000239218 Limulus Species 0.000 abstract 1
- 108060001084 Luciferase Proteins 0.000 abstract 1
- 239000005089 Luciferase Substances 0.000 abstract 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000000825 pharmaceutical preparation Substances 0.000 abstract 1
- 229940127557 pharmaceutical product Drugs 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 239000012086 standard solution Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/12—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar
- G01N2400/24—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar beta-D-Glucans, i.e. having beta 1,n (n=3,4,6) linkages between saccharide units, e.g. xanthan
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/50—Lipopolysaccharides; LPS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Plasma & Fusion (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Provided are a quantification method and a quantification device with which it is possible to measure a sodium -ion- containing sample , such as a biological sample, a pharmaceutical product or food , with high sensitivity and high accuracy. This quantification method comprises: a calibration curve creating step for measuring a standard solution, which has been prepared by adding sodium ions such that the sodium ion content thereof is equivalent to the sodium ion content of a sample to be measured in accordance with a method employing a reaction that activates a Limulus reagent and/or a biochemical luminescent reaction caused by ATP ,luciferin, and luciferase , and for creating a calibration curve that represents the relationship between the measurement value and the amount of a component to be measured; a sample measurement step for measuring said sample to be measured in accordance with the same method as the method used in said calibration curve creating step; and a quantification step for finding , by using said calibration curve , the amount of said component to be measured in said sample to be measured from the measurement value obtained in said sample measurement step.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012207268A JP5903017B2 (en) | 2012-09-20 | 2012-09-20 | Quantitation method, quantification device and quantification kit |
| PCT/JP2013/075304 WO2014046183A1 (en) | 2012-09-20 | 2013-09-19 | Quantification method, quantification device, and quantification kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2015DN02992A true IN2015DN02992A (en) | 2015-09-25 |
Family
ID=50341487
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN2992DEN2015 IN2015DN02992A (en) | 2012-09-20 | 2013-09-19 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20150225768A1 (en) |
| EP (1) | EP2899542A4 (en) |
| JP (1) | JP5903017B2 (en) |
| CN (1) | CN104781673A (en) |
| IN (1) | IN2015DN02992A (en) |
| WO (1) | WO2014046183A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6493316B2 (en) * | 2016-06-21 | 2019-04-03 | 東亜ディーケーケー株式会社 | Mutant beetle luciferase, gene, recombinant vector, transformant, and method for producing mutant beetle luciferase |
| JP6789109B2 (en) * | 2016-12-28 | 2020-11-25 | 富士フイルム株式会社 | Blood analysis method and blood test kit |
| JP7397343B2 (en) * | 2021-06-02 | 2023-12-13 | 東亜ディーケーケー株式会社 | How to measure endotoxin |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3944391A (en) * | 1974-12-27 | 1976-03-16 | Preventive Systems, Inc. | In vitro process for detecting endotoxin in a biological fluid |
| JPS59151899A (en) * | 1983-02-16 | 1984-08-30 | Unitika Ltd | Determination composition |
| JPH07110301A (en) * | 1993-10-12 | 1995-04-25 | Toa Denpa Kogyo Kk | Method and apparatus for measuring atp quantity of cell |
| JPH1128099A (en) * | 1997-05-12 | 1999-02-02 | Kikkoman Corp | Detection of staphylococcus aureus |
| EP0924220A3 (en) * | 1997-12-16 | 2000-04-26 | Wako Pure Chemical Industries, Ltd. | Inhibitor of the activation of beta-glucan recognition protein |
| JP2000131323A (en) * | 1998-10-28 | 2000-05-12 | Fuji Photo Film Co Ltd | Adjusted and controlled serum |
| WO2006080448A1 (en) * | 2005-01-27 | 2006-08-03 | Seikagaku Corporation | Pretreatment agent for limulus test |
| JP2007064895A (en) * | 2005-09-01 | 2007-03-15 | Wako Pure Chem Ind Ltd | Pretreatment method for endotoxin measurement of bio-applicable material and method for measuring endotoxin |
| US8492168B2 (en) * | 2006-04-18 | 2013-07-23 | Advanced Liquid Logic Inc. | Droplet-based affinity assays |
| EP2495321A1 (en) * | 2006-07-07 | 2012-09-05 | Seikagaku Corporation | Pro-clotting enzyme, and method for detection of endotoxin using the same |
| US8394602B2 (en) * | 2007-11-12 | 2013-03-12 | Hiroshima University | Luminescent method for measuring endotoxin |
| JP5686308B2 (en) * | 2009-02-20 | 2015-03-18 | 国立大学法人広島大学 | β-glucan concentration measurement method and concentration measurement kit |
| WO2012029171A1 (en) * | 2010-09-03 | 2012-03-08 | 興和株式会社 | Method for measuring physiologically active substance of biological origin |
-
2012
- 2012-09-20 JP JP2012207268A patent/JP5903017B2/en active Active
-
2013
- 2013-09-19 US US14/429,784 patent/US20150225768A1/en not_active Abandoned
- 2013-09-19 CN CN201380048822.0A patent/CN104781673A/en active Pending
- 2013-09-19 WO PCT/JP2013/075304 patent/WO2014046183A1/en active Application Filing
- 2013-09-19 IN IN2992DEN2015 patent/IN2015DN02992A/en unknown
- 2013-09-19 EP EP13839625.4A patent/EP2899542A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JP5903017B2 (en) | 2016-04-13 |
| CN104781673A (en) | 2015-07-15 |
| JP2014062785A (en) | 2014-04-10 |
| EP2899542A4 (en) | 2016-04-13 |
| EP2899542A1 (en) | 2015-07-29 |
| US20150225768A1 (en) | 2015-08-13 |
| WO2014046183A1 (en) | 2014-03-27 |
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