CN104130036A - Method for preparing bio-organic fertilizer by using bacillus pumilus - Google Patents
Method for preparing bio-organic fertilizer by using bacillus pumilus Download PDFInfo
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- CN104130036A CN104130036A CN201410327186.1A CN201410327186A CN104130036A CN 104130036 A CN104130036 A CN 104130036A CN 201410327186 A CN201410327186 A CN 201410327186A CN 104130036 A CN104130036 A CN 104130036A
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Abstract
The invention relates to a method for preparing a bio-organic fertilizer by using bacillus pumilus. Accrording to the invention, the bacillus pumilus in the bio-organic fertilizer has a strain collection number of CCTCC NO: M2014052. The fertilizer is prepared through the steps of composting, granulating, functional inoculum spray absorption, and low-temperature drying. The bio-organic fertilizer obtained with the method provided by the invention is applied as a base fertilizer, and is used in cotton, corn, melon or cucumber planting. With the fertilizer, yield increase rates are: cotton 7.3%, corn 12.4%, melon 8.2% and cucumber 10.9%, all of which reaches significant difference (P is smaller than 0.05). Also, During the use of the fertilizer, soil is improved. The fertilizer has significant economic, environmental, and social benefits. With the fertilizer, soil can be fertilized, and effects of promoting crop growth and increasing crop yield can be provided.
Description
Technical field
The present invention relates to a kind of bacillus pumilus and prepare the method for biological organic fertilizer, belong to fertilizer field.
Background technology
Biological organic fertilizer refers to that a class that microorganisms with specific functions and main plant and animal residues (as feces of livestock and poultry, agricultural crop straw) are composited for source and through harmless treatment, the organic materials that becomes thoroughly decomposed has the fertilizer of microbial fertilizer and fertilizer effect.
Biological organic fertilizer nutritive element is complete, can improve soil, improves the soil compaction that uses chemical fertilizer to cause.Improve soil physical and chemical property, strengthen the ability of soil water-retaining, fertilizer conservation, fertilizer.Beneficial microorganism in biological organic fertilizer forms mutual symbiosis Relationship With Proliferation with Soil Microorganism after entering soil, suppressing harmful bacteria grows and is converted into probiotics, interact, mutually promote, play the synergy of colony, probiotics produces a large amount of meta-bolitess in growth and breeding process, impels organic decomposition and inversion, can be directly or indirectly for crop provides multiple nutrients and pungent, promote and regulation and control plant growth.Improve soil porosity, penetrating exchangeability and plant survival rate, increase probiotics and soil microorganisms and population.Meanwhile, the advantage probiotics group energy forming at crop root suppresses harmful pathogenic bacteria procreation, strengthens the degeneration-resistant resistance against diseases of crop and reduces the disease index of continuous cropping crop, uses year after year and can greatly alleviate continuous cropping obstacle.
Summary of the invention
The object of this invention is to provide a kind of bacillus pumilus strain and prepare the method for biological organic fertilizer, the culture presevation number of the bacillus pumilus Bacillus pumilus described in the method in biological organic fertilizer is CCTCC NO:M2014052; Adopt compost, granulation, spraying adsorption function microbial inoculum and low temperature drying method to make; The biological organic fertilizer that the biological organic fertilizer obtaining by the method for the invention obtains by the method for the invention is for cotton, corn, muskmelon or green cucumber, amount of increase in production is 7.3%, 12.4%, 8.2%, 10.9%, all reaches significant difference (P<0.05); Meanwhile, improved soil in use procedure, its economic benefit, environmental benefit and social benefit are obvious.Can fertilizing soil, there is promotion plant growth simultaneously, improve effect of crop yield.
A kind of method of utilizing bacillus pumilus to prepare biological organic fertilizer of the present invention, the culture presevation number of the bacillus pumilus Bacillus pumilus in described biological organic fertilizer is CCTCC NO:M2014052; Concrete operations follow these steps to carry out:
A, be just to mix with the dry cow dung of 70 parts after the weathered coal of 25 parts, the urea fragmentation of 2 parts by weight, then the water ratio in compound is adjusted to 60%, carry out compost fermentation, heap temperature control, between 30-65 DEG C, exceedes 65 DEG C, carries out turning, compost 30 days, treats that raw material becomes thoroughly decomposed completely;
B, will in step a, become thoroughly decomposed after raw material pulverizing, cross 100 mesh sieves and make powder raw material, then in powder raw material, add the sulphur gypsum of 3 parts of weight, after mixing, carry out according to a conventional method granulation, temperature 120-210 DEG C is dried to water ratio 8%, then after particulate material is cooling, makes particulate material;
The particulate material that c obtains step b is sprayed with the function microbial inoculum of quality 5%, throws circle after particulate material adsorption function microbial inoculum, dries to water ratio 8% 55 DEG C of temperature, packs, and obtains bacillus pumilus biological organic fertilizer.
The microbial inoculum of function described in step c is peptone 10g, yeast powder 3.5g, potassium primary phosphate 0.3g, ammonium nitrate 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.02g, zinc sulfate 0.001g, manganous sulfate 0.002g, water 1000ml, pH value 7.2, according to a conventional method preparation.
A kind of method of utilizing bacillus pumilus to prepare biological organic fertilizer of the present invention, the GCH-3 of bacillus pumilus described in the method (Bacillus pumilusGCH-3) is on February 28th, 2014 in the Chinese Typical Representative culture collection center preservation of Wuchang road, Wuhan, Hubei Jia Shan Wuhan University, and culture presevation number is CCTCC NO:M2014052.
The present invention compared with prior art, provides a kind of new bacillus pumilus, utilizes biological organic fertilizer prepared by bacillus pumilus to have the following advantages and effect:
(1) biological organic fertilizer of the present invention is rich in organic matter, can improve Soil structure, improves quality of agricultural product, mouthfeel and nutrition;
(2) production technique of biological organic fertilizer adopts second stage drying method, after adsorption function bacterium, adopts low temperature drying technology, and the amount of survival of function yeast ensures to some extent, this biological organic fertilizer is granular simultaneously, can adopt machine sowing fertilising, be conducive to mechanize, the work that reduces peasant drops into;
(3) this biological organic fertilizer application has obvious yield increasing effect, and amount of increase in production reaches more than 7.3%.
Brief description of the drawings
Fig. 1 is production technique figure of the present invention.
Embodiment
Function stem of the present invention is bacillus pumilus GCH-3 (Bacillus pumilus GCH-3), be preserved in Jia Shan Wuhan University Chinese Typical Representative culture collection center, Wuchang road, Wuhan, Hubei on February 28th, 2014, culture presevation number is CCTCC NO:M2014052.
Embodiment 1
The preparation of function microbial inoculum:
Primary seed solution enlarged culturing: first order seed liquid culture medium prescription: peptone 10g, yeast powder 3.5g, potassium primary phosphate 0.3g, ammonium nitrate 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.02g, zinc sulfate 0.001g, manganous sulfate 0.002g, water 1000ml, pH value 7.2, the bacillus pumilus that purifying is cultivated is seeded to above-mentioned seed liquor enlarged culturing base, 37 DEG C of culture temperature from the slant medium of preservation, on shaking table, cultivate, rotating speed 180rpm, cultivates 24h, and bacterium is counted content and reaches 1 × 109/ml;
Secondary seed solution enlarged culturing: by by volume 5% inoculum size access of first order seed fermented liquid 200L fermentor tank, fermention medium is consistent with first order seed liquid culture medium, 37 DEG C of culture temperature, be aided with 250 revs/min of rotating speeds simultaneously, air flow 0.5 cubic meters per minute, carry out fermentation culture, cultivate 24h, bacterium is counted content and reaches 1 × 109/ml;
The fermentation of function microbial inoculum: by by volume 10% inoculum size access of secondary seed fermented liquid 2000L fermentor tank, fermention medium is consistent with first order seed liquid culture medium, 37 DEG C of culture temperature, be aided with 250 revs/min of rotating speeds simultaneously, air flow 2.5 cubic meters per minute, carry out fermentation culture, cultivate 36h, bacterium is counted content and reaches 1 × 109/ml, for subsequent use;
The preparation of bacillus pumilus biological organic fertilizer:
Be just to mix with the dry cow dung of 70 parts after the weathered coal of 25 parts, the urea fragmentation of 2 parts by weight, then the water ratio in compound is adjusted to 60%, carry out compost fermentation, heap temperature control is at 30 DEG C, while exceeding 65 DEG C of temperature, carry out turning, compost 30 days, treats that raw material becomes thoroughly decomposed completely;
To become thoroughly decomposed after raw material pulverizing, and cross 100 mesh sieves and make powder raw material, then in powder raw material, add the sulphur gypsum of 3 parts of weight, after mixing, carry out according to a conventional method granulation, 120 DEG C of temperature are dried to water ratio 8%, then after particulate material is cooling, make particulate material;
The particulate material obtaining is sprayed with the function microbial inoculum of having prepared of quality 5%, after particulate material adsorption function microbial inoculum, throw circle, dry to water ratio 8% 55 DEG C of temperature, pack, obtain bacillus pumilus biological organic fertilizer.
Embodiment 2
The preparation of function microbial inoculum is carried out according to embodiment 1;
The preparation of bacillus pumilus biological organic fertilizer:
Be just to mix with the dry cow dung of 70 parts after the weathered coal of 25 parts, the urea fragmentation of 2 parts by weight, then the water ratio in compound is adjusted to 60%, carry out compost fermentation, heap temperature control is at 45 DEG C, while exceeding 65 DEG C of temperature, carry out turning, compost 30 days, treats that raw material becomes thoroughly decomposed completely;
To become thoroughly decomposed after raw material pulverizing, and cross 100 mesh sieves and make powder raw material, then in powder raw material, add the sulphur gypsum of 3 parts of weight, after mixing, carry out according to a conventional method granulation, 170 DEG C of temperature are dried to water ratio 8%, then after particulate material is cooling, make particulate material;
The particulate material obtaining is sprayed with the function microbial inoculum of having prepared of quality 5%, after particulate material adsorption function microbial inoculum, throw circle, dry to water ratio 8% 55 DEG C of temperature, pack, obtain bacillus pumilus biological organic fertilizer.
Embodiment 3
The preparation of function microbial inoculum is carried out according to embodiment 1;
The preparation of bacillus pumilus biological organic fertilizer:
Be just to mix with the dry cow dung of 70 parts after the weathered coal of 25 parts, the urea fragmentation of 2 parts by weight, then the water ratio in compound is adjusted to 60%, carry out compost fermentation, heap temperature control is at 65 DEG C, while exceeding 65 DEG C of temperature, carry out turning, compost 30 days, treats that raw material becomes thoroughly decomposed completely;
To become thoroughly decomposed after raw material pulverizing, and cross 100 mesh sieves and make powder raw material, then in powder raw material, add the sulphur gypsum of 3 parts of weight, after mixing, carry out according to a conventional method granulation, 210 DEG C of temperature are dried to water ratio 8%, then after particulate material is cooling, make particulate material;
The particulate material obtaining is sprayed with the function microbial inoculum of having prepared of quality 5%, after particulate material adsorption function microbial inoculum, throw circle, dry to water ratio 8% 55 DEG C of temperature, pack, obtain bacillus pumilus biological organic fertilizer.
The effect of embodiment 4 biological organic fertilizers on crop-planting.
Any one bacillus pumilus biological organic fertilizer that embodiment 1-3 is obtained is used as base manure, for cotton, corn, muskmelon or green cucumber, use by every mu of 80kg, its result shows: four kinds of crops of using biological organic fertilizer of the present invention are cotton 7.3% with respect to the amount of increase in production of peasant habit fertilising, corn 12.4%, muskmelon 8.2%, cucumber 10.9%, all reaches significant difference (P<0.05); Meanwhile, improved soil in use procedure, its economic benefit, environmental benefit and social benefit are obvious.
Claims (2)
1. utilize bacillus pumilus to prepare a method for biological organic fertilizer, it is characterized in that the culture presevation number of the bacillus pumilus Bacillus pumilus in described biological organic fertilizer is CCTCC NO:M2014052; Concrete operations follow these steps to carry out:
A, be just to mix with the dry cow dung of 70 parts after the weathered coal of 25 parts, the urea fragmentation of 2 parts by weight, then the water ratio in compound is adjusted to 60%, carry out compost fermentation, heap temperature control is at 30-65 DEG C, while exceeding 65 DEG C of temperature, carry out turning, compost 30 days, treats that raw material becomes thoroughly decomposed completely;
B, will in step a, become thoroughly decomposed after raw material pulverizing, cross 100 mesh sieves and make powder raw material, then in powder raw material, add the sulphur gypsum of 3 parts of weight, after mixing, carry out according to a conventional method granulation, temperature 120-210 DEG C is dried to water ratio 8%, then after particulate material is cooling, makes particulate material;
The particulate material that c obtains step b is sprayed with the function microbial inoculum of quality 5%, throws circle after particulate material adsorption function microbial inoculum, dries to water ratio 8% 55 DEG C of temperature, packs, and obtains bacillus pumilus biological organic fertilizer.
2. method according to claim 1, it is characterized in that function microbial inoculum described in step c is peptone 10g, yeast powder 3.5g, potassium primary phosphate 0.3g, ammonium nitrate 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.02g, zinc sulfate 0.001g, manganous sulfate 0.002g, water 1000ml, pH value 7.2, according to a conventional method preparation.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105016929A (en) * | 2015-08-11 | 2015-11-04 | 新疆农业科学院土壤肥料与农业节水研究所 | Water retention type humic acid bio-organic fertilizer and preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101538174A (en) * | 2009-01-20 | 2009-09-23 | 新疆惠森生物技术有限公司 | Method and special bacteria-adding equipment for adding bacteria after preparing bio-organic fertilizer |
| CN101676243A (en) * | 2008-09-19 | 2010-03-24 | 辽宁元亨生物科技有限公司 | Production process of biological-organic-inorganic ternary compound fertilizer |
| CN102888356A (en) * | 2012-09-03 | 2013-01-23 | 新疆农业科学院土壤肥料与农业节水研究所 | Bacillus subtilis, microbial fertilizer prepared by using bacillus subtilis, method for preparing microbial fertilizer by using bacillus subtilis, and application of microbial fertilizer |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101676243A (en) * | 2008-09-19 | 2010-03-24 | 辽宁元亨生物科技有限公司 | Production process of biological-organic-inorganic ternary compound fertilizer |
| CN101538174A (en) * | 2009-01-20 | 2009-09-23 | 新疆惠森生物技术有限公司 | Method and special bacteria-adding equipment for adding bacteria after preparing bio-organic fertilizer |
| CN102888356A (en) * | 2012-09-03 | 2013-01-23 | 新疆农业科学院土壤肥料与农业节水研究所 | Bacillus subtilis, microbial fertilizer prepared by using bacillus subtilis, method for preparing microbial fertilizer by using bacillus subtilis, and application of microbial fertilizer |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105016929A (en) * | 2015-08-11 | 2015-11-04 | 新疆农业科学院土壤肥料与农业节水研究所 | Water retention type humic acid bio-organic fertilizer and preparation method |
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Inventor after: Ge Chunhui Inventor after: Yu Yaofeng Inventor after: Yan An Inventor after: Geng Kui Inventor after: Wang Farong Inventor after: Zheng Wei Inventor after: Sun Jiusheng Inventor after: Ma Yanru Inventor after: Wang Xinyong Inventor before: Ge Chunhui Inventor before: Sun Jiusheng Inventor before: Ma Yanru Inventor before: Wang Xinyong Inventor before: Yang Jinyu Inventor before: Li Xiaofei Inventor before: Wang Bing Inventor before: Zhu Li |
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Effective date of registration: 20160215 Address after: 831200, the Xinjiang Uygur Autonomous Region, Hutubi County Tianshan Industrial Park light textile industry building on the east side of building eight Applicant after: Xinjiang Baoli Xingnong Biological Co.,Ltd. Address before: 830091 Nanchang Road, the Xinjiang Uygur Autonomous Region, No. 403, No. Applicant before: INSTITUTE OF SOIL AND FERTILIZER, XINJIANG ACADEMY OF AGRICULTURAL SCIENCES |
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