CN104094773A - Method for cultivating glossy ganoderma by rectangular boxes - Google Patents
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- CN104094773A CN104094773A CN201410376455.3A CN201410376455A CN104094773A CN 104094773 A CN104094773 A CN 104094773A CN 201410376455 A CN201410376455 A CN 201410376455A CN 104094773 A CN104094773 A CN 104094773A
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Abstract
The invention discloses a method for cultivating glossy ganoderma by rectangular boxes. The method includes building a culture room with environmental factors controlled, cultivating glossy ganoderma in rectangular boxes indoors, sealing the rectangular boxes when glossy ganoderma matures, ejecting glossy ganoderma spore powder in the rectangular boxes, and collecting the glossy ganoderma spore powder in the rectangular boxes and on glossy ganoderma caps after finishing ejection. Since the rectangular boxes are utilized for culture of glossy ganoderma, a clean growth environment is provided for glossy ganoderma, pollution is reduced, environmental factors can be controlled effectively, and recovery ratio of the glossy ganoderma spore powder is close to 100% after the rectangular boxes are sealed during glossy ganoderma spore powder collection. Besides, as the rectangular boxes are made of glass and smooth fabric, the spore powder ejected to the boxes can be collected conveniently with time and labor saved; the spore powder is ejected inside the clean rectangular boxes, so that the collected spore powder has high purity without foreign substance.
Description
Technical field
The invention belongs to cultivation of glossy ganoderma technical field, particularly a kind of by the method for cuboid box cultivating ganoderma.
Background technology
Glossy ganoderma (formal name used at school: Ganoderma Lucidum Karst), claim again polyporus lucidus, celestial grass, red sesame, red sesame, Ganoderma Lucidum, Ganoderma, 10000 years gill fungus, polyporus lucidus have another name called, it is the complete stool of the red sesame of Polyporaceae plant or purple sesame, for the fruit body of On Polyporaceae glossy ganoderma, it is the treasure in motherland's pools of traditional Chinese medicine.Profile is umbrella, cap kidney shape, semicircle or subcircular.Glossy ganoderma, as the China's tradition valuable ingredient of traditional Chinese medicine that has thousands of years medicinal histories, has invigorating qi for tranquilization, relieving cough and asthma effect, and for dizzy egersis, palpitation, consumptive disease is coughed and is breathed heavily.Glossy ganoderma possesses very high medical value, through the modern pharmacology of scientific research institution many decades, studies confirm that, glossy ganoderma, for strengthening body immunity, regulates blood sugar, controls blood pressure, auxiliary tumor chemoradiotherapy, and liver protecting, promotes the aspects such as sleep all to have significant curative effect.GL-B is one of main active ingredient of glossy ganoderma medicinal function, has biologically active widely, as strengthened immunity, remove human free radical, protect cardiovascular, radioresistance, protection liver, the effect such as antitumor.Triterpenes is also one of active ingredient of glossy ganoderma, and not lower over one hundred kind of the contained triterpenes of glossy ganoderma, wherein take tetracyclic triterpenes as main, the bitter taste of glossy ganoderma is relevant with contained triterpenes, human hepatoma cell is had to cytotoxicity, also can suppress the release of histamine, have and protect the liver and the effects such as antiallergy.Glossy ganoderma is formally recorded by NF as medicine now, and within 2005, American Pharmacopeia has formally been included glossy ganoderma, and it is again the new resource food of state approval simultaneously, has no side effect, can medicine-food two-purpose.
Because the glossy ganoderma of self-sow is few, and contain a large amount of raw fiber and other impurity, and ganoderma polyoses content not high (being generally 2-5%) is not easy to directly as health food or pharmaceutical raw material, therefore, many scientific workers carry out artificial cultivation and the culture technique research of glossy ganoderma.In Ganoderma Lucidum process, high yield and the purity of lucidum spore powder, be a great problem that glossy ganoderma is gathered.As Chinese patent application 201310558173.0 discloses the acquisition methods of a kind of land for growing field crops facility cultivation of glossy ganoderma and lucidum spore powder, the method is rationally controlled environmental factor by facility, and adopt and to build conidial powder and gather that canopy mode substitutes original independent sleeve or bagging method gathers conidial powder, this conidial powder gathers canopy and becomes a sealing, protection against the tide, ventilative conidial powder collection environment with cloth (umbrella cloth) by the scraps of paper or film.Three kinds of methods that gather lucidum spore powder in the contrast of disclosed three kinds of high-quality lucidum spore powder collecting methods such as Qian Youan, have been mentioned, be respectively a layer frame bagging method, plant bagging method, equidistant perpendicular cultivation bagging method, these methods can be collected conidial powder, but need to spend more manpower and time, and the collection rate of lucidum spore powder is not high enough, and the lucidum spore powder purity of collecting is not high.Study the high and high-quality of a kind of glossy ganoderma output, can improve the ganoderma lucidum cultivation method of recovery ratio and the purity of lucidum spore powder, become the important need in cultivation of glossy ganoderma field.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of by the method for cuboid box cultivating ganoderma, and the method is convenient collects lucidum spore powder, and the conidial powder purity of collecting is high.
For achieving the above object, the present invention adopts technical scheme as follows:
By a method for cuboid box cultivating ganoderma, comprise the following steps:
(1) culturing room builds: the size of culturing room is set to 100～120m
3/; Cultivate indoor location temperature controller, culturing room's surrounding arranges ventilation window, at culturing room's surroundings wall and top, fluorescent tube is installed, and sprinkling water and spraying fog development is installed at top; Cultivate partition and place cuboid box, the length in cuboid box length and room or wide matched, width is 80～100cm, be highly 80～100cm, at cuboid box madial wall, apart from top, 5～10cm place loads onto watering sprayer, and described cuboid box top and length direction two ends are movable;
(2) go out the selection of sesame medium: with the polythene material plastic sack of 26 * 40 * 0.05cm specification, as container, divide routinely and install culture matrix material, then by sterilization, inoculation, manage to mycelia and cover with matrix, mycelia annesl; From container, select mycelia covered with matrix and also annesl, FFI medium as going out sesame medium;
(3) go out sesame management: sesame medium is stacking to be swung in culturing room's cuboid box going out of choosing, go out sesame medium two ends apart from box body wall same distance, going out sesame medium is stacked to apart from cuboid box top 25～35cm, go out between sesame medium, to put compactness and do not interspace, manage according to a conventional method to glossy ganoderma and gather;
(4) conidial powder and fruit body are gathered: treat that sesame lid expands to maximum, when bright orange edge disappears, cuboid box is all sealed, for conidial powder, launch, controlling growing environment temperature is 26 ℃～28 ℃, and humidity is 65%～70%; After conidial powder is launched end, collect drop on cuboid box inwall and cap on conidial powder, finally carry out ganoderma lucidum fruitbody and gather.
For reducing insect pest, ventilation window of the present invention is loaded onto fly net.
Fluorescent tube wattage of the present invention is preferably 15～20 watts.
For making personnel can carry out out sesame management work, guarantee the circulation of air in glossy ganoderma culturing room, the spacing between cuboid box of the present invention is preferably 40～50cm.
In order to save manpower and time, convenient collection launched the lucidum spore powder in cuboid box, and simultaneously in order to guarantee the light transmittance of glossy ganoderma, the active face of cuboid box of the present invention is white smooth cloth, and other faces are colourless transparent glass material.
In glossy ganoderma process of growth, for glossy ganoderma can better be ventilated, prevent that carbonic acid gas is too high and medium is mouldy, before the conidial powder of gathering, the active face of cuboid box of the present invention keeps opening-wide state.
Compared with prior art, beneficial effect of the present invention is:
1, the present invention is at indoor location temperature controller, fluorescent tube and sprinkling water and spraying fog development, and better temperature, luminosity and the humidity of the growth of control effect glossy ganoderma, avoid glossy ganoderma to be subject to the impact of environmental factor, is conducive to glossy ganoderma output and improves and steady quality.Meanwhile, fly net on culturing room's windows, can effectively avoid insect pest, protection glossy ganoderma.
2, the present invention adopts cuboid box cultivating ganoderma, for glossy ganoderma growth provides the environment of clean hygiene, avoids medium directly to contact with soil with glossy ganoderma, reduces or prevents heavy metal pollution.When glossy ganoderma is launched conidial powder, sealing cuboid box, lucidum spore powder only launches in cuboid box, the collection rate of lucidum spore powder approaches absolutely, and medium and glossy ganoderma are in indoor cuboid box, clean, the conidial powder of launching is not easy by contaminating impurity, and the purity of conidial powder is improved.Cuboid box is white smooth cloth and colourless transparent glass material, and for glossy ganoderma growth provides the environment of ventilative a, printing opacity, and convenient collection launched the lucidum spore powder in cuboid box, saves manpower and time.
3, adopt cultivation method of the present invention, the ganoderma polyoses content in the glossy ganoderma of cultivation and glossy ganoderma output are than the height of booth.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to these embodiment.
Embodiment 1
By a method for cuboid box cultivating ganoderma, comprise the following steps:
(1) culturing room builds: the size of culturing room is set to 100m
3/; Cultivate indoor location temperature controller, culturing room's surrounding arranges ventilation window, loads onto the fly net matching at ventilation window, wattage is installed is the fluorescent tube of 15 watts at culturing room's surroundings wall and top, and sprinkling water and spraying fog development is installed at top; Cultivate partition and place cuboid box, adjacent spacing is 40cm, the length in cuboid box length and room or wide matched, width is 80cm, is highly 80cm, at cuboid box madial wall, apart from top, 5cm place loads onto watering sprayer, cuboid box top and length direction two ends are movable, and the active face of cuboid box is white smooth cloth, and other faces are colourless transparent glass material;
(2) preparation of medium: the raw material that takes following percentage by weight: maple wood chip 35%, cotton seed hulls 15%, wheat bran 12%, rice bran 10%, sucrose 1.2%, calcium carbonate 0.8%, gypsum 0.5%, magnesium carbonate 0.7%, water content 24.8%, maple wood chip is become to the wood grain of 1cm by crusher in crushing, then mix with other raw materials, with the polythene material plastic sack of 26 * 40 * 0.05cm specification as container, by dividing and install culture matrix material by 500g/ bag, 120 ℃ of temperature, under 1.2MPa pressure condition, sterilizing is 5 hours, treat that temperature is cooled to 27 ℃, standby,
(3) inoculation: give standby packed culture medium inoculated lucidum strain in aseptic inoculation room;
(4) cultural hypha: by the stacking discharge in cultivating room of postvaccinal culture bag, cultivating room temperature control is 27 ℃, humidity is 65%, ventilate every day 1 hour, lucifuge, when mycelia, cover with matrix, mycelia annesl, select mycelia covered with matrix and also annesl, FFI medium as going out sesame medium;
(5) go out sesame management: by sesame medium is stacking to be swung in culturing room's cuboid box going out of choosing, go out sesame medium two ends apart from box body wall same distance, going out sesame medium is stacked to apart from cuboid box top 25cm, go out between sesame medium, to put compactness and do not interspace, cultivation temperature is 27 ℃, and relative moisture is 70%, illumination is 450Lx, ventilate every day 1 hour, before the conidial powder of gathering, the active face of cuboid box keeps opening-wide state;
(6) conidial powder and fruit body are gathered: treat that sesame lid expands to maximum, when bright orange edge disappears, cuboid box is all sealed, for conidial powder, launch, controlling growing environment temperature is 26 ℃, and humidity is 65%; After conidial powder is launched end, collect drop on cuboid box inwall and cap on conidial powder, finally carry out ganoderma lucidum fruitbody and gather.
Embodiment 2
By a method for cuboid box cultivating ganoderma, comprise the following steps:
(1) culturing room builds: the size of culturing room is set to 120m
3/; Cultivate indoor location temperature controller, culturing room's surrounding arranges ventilation window, loads onto the fly net matching at ventilation window, wattage is installed is the fluorescent tube of 20 watts at culturing room's surroundings wall and top, and sprinkling water and spraying fog development is installed at top; Cultivate partition and place cuboid box, adjacent spacing is 50cm, the length in cuboid box length and room or wide matched, width is 100cm, is highly 100cm, at cuboid box madial wall, apart from top, 10cm place loads onto watering sprayer, cuboid box top and length direction two ends are movable, and the active face of cuboid box is white smooth cloth, and other faces are colourless transparent glass material;
(2) preparation of medium: the raw material that takes following percentage by weight: maple wood chip 40%, cotton seed hulls 10%, corn flour 12%, wheat bran 10%, rice bran 12%, sucrose 0.8%, calcium carbonate 1.0%, gypsum 0.7%, magnesium carbonate 0.8%, water content 12.7%, maple wood chip is become to the wood grain of 2cm by crusher in crushing, then mix with other raw materials, with the polythene material plastic sack of 26 * 40 * 0.05cm specification as container, by dividing and install culture matrix material by 500g/ bag, 115 ℃ of temperature, under 1.0MPa pressure condition, sterilizing is 6 hours, treat that temperature is cooled to 25 ℃, standby,
(3) inoculation: give standby packed culture medium inoculated lucidum strain in aseptic inoculation room;
(4) cultural hypha: by the stacking discharge in cultivating room of postvaccinal culture bag, cultivating room temperature control is 28 ℃, humidity is 60%, ventilate every day 2 hours, lucifuge, when mycelia, cover with matrix, mycelia annesl, select mycelia covered with matrix and also annesl, FFI medium as going out sesame medium;
(5) go out sesame management: by sesame medium is stacking to be swung in culturing room's cuboid box going out of choosing, go out sesame medium two ends apart from box body wall same distance, going out sesame medium is stacked to apart from cuboid box top 35cm, go out between sesame medium, to put compactness and do not interspace, cultivation temperature is 28 ℃, and relative moisture is 65%, illumination is 500Lx, ventilate every day 2 hours, before the conidial powder of gathering, the active face of cuboid box keeps opening-wide state;
(6) conidial powder and fruit body are gathered: treat that sesame lid expands to maximum, when bright orange edge disappears, cuboid box is all sealed, for conidial powder, launch, controlling growing environment temperature is 28 ℃, and humidity is 70%; After conidial powder is launched end, collect drop on cuboid box inwall and cap on conidial powder, finally carry out ganoderma lucidum fruitbody and gather.
Comparative example
By a method for greenhouse cultivation glossy ganoderma, preparation process is as follows:
(1) booth is built: every 1～2 meter of flexible pole stock of use, be barricaded as arc canopy, the height of arc canopy is 1.5 meters, 20 meters of length, 2.5 meters of width, arc canopy has two-layer ceiling, internal layer ceiling plastic covering film, outer ceiling covered with sunshade net, internal layer top arranges sprinkling water and spraying fog development, and arc canopy two ends arrange mobilizable plastic foil;
(2) wood chip is processed in earlier stage: by being crushed to particle diameter after the trunk of maple after felling and branch peeling, be 1cm, dry standby;
(3) batching: the raw material that takes following percentage by weight: maple wood chip 40%, cotton seed hulls 10%, corn flour 12%, wheat bran 10%, rice bran 12%, sucrose 0.8%, calcium carbonate 1.0%, gypsum 0.7%, magnesium carbonate 0.8%, water content 12.7%;
(4) spice: first, by maple wood grain and other raw material blendings, then add water and fully mix formation medium matrix thoroughly, amount of water is held webs water breakthrough with hand grip but do not formed the globule;
(5) pack: after spice finishes, windrow 1 hour, makes moisture fully be absorbed by medium matrix, selects the polythene material plastic sack of 26 * 40 * 0.05cm specification as container, material bag two tying, sack is noted cleaning, and prevents later stage living contaminants;
(6) sterilizing: material bag is put into sterilizing installation, and sterilizing is 6 hours under 115 ℃ of temperature, 1.0MPa pressure condition, treats that temperature is cooled to 28 ℃, standby;
(7) inoculation: give standby packed culture medium inoculated lucidum strain in aseptic inoculation room;
(8) cultural hypha: by the stacking discharge in cultivating room of postvaccinal culture bag, cultivating room temperature control is 28 ℃, humidity is 60%, give every day and ventilate 2 hours, lucifuge, when mycelia, cover with matrix, mycelia annesl, select mycelia covered with matrix and also annesl, FFI medium as going out sesame medium;
(9) go out sesame management: by sesame medium is stacking to be swung in outdoor booth going out of choosing, go out between sesame medium, to put compactness and do not interspace, cultivation temperature is 28 ℃, relative moisture is 65%, illumination is 500Lx, ventilates every day 2 hours, manages to glossy ganoderma maturation and gathers.
(10) conidial powder and fruit body are gathered: treat that sesame lid expands to maximum, when bright orange edge disappears, is enclosed within clean bag film on glossy ganoderma; After conidial powder is launched end, collect drop on cap and bag film in conidial powder, finally carry out ganoderma lucidum fruitbody and gather.
Test example
Adopt the test comparison of GL-B, content of beary metal in the glossy ganoderma of the cultivation of the method by cuboid box cultivating ganoderma of the present invention and the glossy ganoderma of greenhouse cultivation to the results are shown in Table 1.
GL-B in the glossy ganoderma that table 1 distinct methods cultivates, the test comparison of content of beary metal
From table 1 result, find out, the content of beary metal of comparative example is more than embodiment 1 and embodiment's 2, and the content of its GL-B is less, show to adopt of the present invention by the method cultivating ganoderma of cuboid box cultivating ganoderma, can effectively reduce heavy metal pollution, can also improve ganoderma polyoses content simultaneously.
Adopt the comparing result of the method by cuboid box cultivating ganoderma of the present invention and greenhouse cultivation method collection lucidum spore powder amount in Table 2.
The different cultivation methods of table 2 are collected the comparing result of lucidum spore powder
From table 2 fruit, find out, the collecting amount of the lucidum spore powder of comparative example is fewer than embodiment 1 and embodiment's 2, shows to adopt the method for cuboid box cultivating ganoderma of passing through of the present invention, can improve the recovery ratio of lucidum spore powder.
Adopt the comparing result of the glossy ganoderma of the cultivation of the method by cuboid box cultivating ganoderma of the present invention and the glossy ganoderma output of greenhouse cultivation method cultivation in Table 3.
The comparing result of the different cultivation method cultivating ganoderma of table 3 output
As seen from Table 3, the glossy ganoderma output of comparative example is fewer than embodiment 1 and embodiment's 2, shows to adopt the method for cuboid box cultivating ganoderma of passing through of the present invention, can improve the output of glossy ganoderma.
Claims (6)
1. by a method for cuboid box cultivating ganoderma, it is characterized in that, comprise the following steps:
(1) culturing room builds: the size of culturing room is set to 100～120m
3/; Cultivate indoor location temperature controller, culturing room's surrounding arranges ventilation window, at culturing room's surroundings wall and top, fluorescent tube is installed, and sprinkling water and spraying fog development is installed at top; Cultivate partition and place cuboid box, the length in cuboid box length and room or wide matched, width is 80～100cm, be highly 80～100cm, at cuboid box madial wall, apart from top, 5～10cm place loads onto watering sprayer, and described cuboid box top and length direction two ends are movable;
(2) go out the selection of sesame medium: with the vinyl material plastic sack of 26 * 40 * 0.05cm specification, as container, divide routinely and install culture matrix material, then by sterilization, inoculation, manage to mycelia and cover with matrix, mycelia annesl; From container, select mycelia covered with matrix and also annesl, FFI medium as going out sesame medium;
(3) go out sesame management: sesame medium is stacking to be swung in culturing room's cuboid box going out of choosing, go out sesame medium two ends apart from box body wall same distance, going out sesame medium is stacked to apart from cuboid box top 25～35cm, go out between sesame medium, to put compactness and do not interspace, manage according to a conventional method to glossy ganoderma and gather;
(4) conidial powder and fruit body are gathered: treat that sesame lid expands to maximum, when bright orange edge disappears, cuboid box is all sealed, for conidial powder, launch, controlling growing environment temperature is 26 ℃～28 ℃, and humidity is 65%～70%; After conidial powder is launched end, collect drop on cuboid box inwall and cap on conidial powder, finally carry out ganoderma lucidum fruitbody and gather.
2. according to claim 1 by the method for cuboid box cultivating ganoderma, it is characterized in that: described ventilation window is loaded onto fly net.
3. according to claim 1 by the method for cuboid box cultivating ganoderma, it is characterized in that: described fluorescent tube wattage is 15～20 watts.
4. according to claim 1 by the method for cuboid box cultivating ganoderma, it is characterized in that: the spacing between described cuboid box is 40～50cm.
5. according to claim 1 by the method for cuboid box cultivating ganoderma, it is characterized in that: the active face of described cuboid box is white smooth cloth, and other faces are colourless transparent glass material.
6. according to claim 1 by the method for cuboid box cultivating ganoderma, it is characterized in that: before the conidial powder of gathering, the active face of cuboid box keeps opening-wide state.
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| CN106797799A (en) * | 2015-11-25 | 2017-06-06 | 浙江超奇农业开发有限公司 | A kind of cultural method of ganoderma lucidum |
| CN108703019A (en) * | 2018-04-17 | 2018-10-26 | 六安市金安区汪氏综合养殖专业合作社 | A kind of the sealing cultivation case and its cultural method of ganoderma lucidum |
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