CN104082348A - Bio-pesticide for preventing and treating rice diseases and preparation method of bio-pesticide - Google Patents
Bio-pesticide for preventing and treating rice diseases and preparation method of bio-pesticide Download PDFInfo
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Abstract
The invention discloses a bio-pesticide for preventing and treating rice diseases and a preparation method of the bio-pesticide. Currently, food safety is globally concerned and safety of a source is the key to food safety, so that preparation of a pesticide which can safely, effectively and conveniently prevent and treat spike neck blast is an important subject in the technical field of the pesticide. The bio-pesticide disclosed by the invention is prepared from Chinese herbal medicines including herb of manchurian wildginger and common cnidium fruit; a field trial shows that the bio-pesticide has the characteristics of good disease prevention effect, safety and environmental friendliness, small possibility of generating pesticide resistance and the like, and an application prospect is wide.
Description
Technical field
The invention belongs to pesticide production technology field, relate to a kind of biopesticide, particularly relate to a kind of biopesticide of preventing and treating rotten neck.
Background technology
Rice blast has another name called rice blast, burns pest, the pest of kowtowing, and is worldwide fungal disease, and there is the generation of this disease in the nearly all rice cropping of China area.At present, the prophylactico-therapeutic measures of rice blast mainly comprises disease-resistant variety seed selection, chemical control and cultivation management.Wherein mostly disease-resistant variety is the vertical resistance kind based on " gene pairs gene " theory basis, easily loses disease resistance.Chemical control has economy, the advantage such as efficient, convenient and rapid, disease be very popular or variety resistance lose during topmost means of prevention especially, but not only contaminated environment of chemical control, and make farm chemical ingredients residual in paddy rice too high, human body is damaged, improve the ill probability of human body, even caused the immunocompetence of the each function of human body to decline.
At present, food security has become the focus of global concern, and the key of food security is the safety in source, therefore, the agricultural chemicals of seek safety, preventing and treating effectively, easily rotten neck is the important topic of technical field of pesticide, contained not only effectively preventing rotten neck of various useful active component in biopesticide, can also play the effect that promotes paddy growth, the full grains of impelling paddy rice to bear, stature is large, weight foot.
Summary of the invention
The object of the invention is to provide a kind of control efficiency good, compatibility is simple, promotes paddy growth, without a kind of biopesticide of preventing and treating rice disease of any residue of pesticide.
Solving the technical scheme that its technical problem adopts is, a kind of biopesticide of preventing and treating rice disease, and this kind of biopesticide is made up of the Chinese herbal medicine root of Chinese wild ginger, frutus cnidii, concrete consists of root of Chinese wild ginger 30-50 part, frutus cnidii 20-30 part; Preferably, be 40 parts of the roots of Chinese wild ginger, 25 parts of frutus cnidiis.
Preparation method is: get the 400g root of Chinese wild ginger, 250g frutus cnidii, pulverize through micropulverizer, cross 100 mesh sieves, add 95% ethanol 500-600ml, after soaking 6h under room temperature, pour out ethanol, so repeatedly carry out 3 times, merge the leaching liquor of 3 times, obtain approximately 200～250ml of leaching liquor, and then 60 DEG C of water-baths are concentrated into 50ml, for subsequent use after ethanol is volatilized completely.
Beneficial effect: it is good that the distinctive formula formulation of the present invention has protection effect, safety and environmental protection, the feature such as be difficult for developing immunity to drugs, has a extensive future; In prescription, the root of Chinese wild ginger, frutus cnidii all have the function of disinfection and deinsectization, and rotten neck is had to good preventive effect, and meanwhile, other active components that contain have more benefit for the growth of paddy rice, in control rice blast.Also there is the effect of foliar application, strengthen the photosynthesis of rice leaf, promote paddy growth; Biopesticide of the present invention is safer to people and animals, and to eyes, skin nonirritant, toxicity is lower, in atmosphere and soil, easily decomposes, and non-poisonous material is residual; Biopesticide of the present invention is little on the impact of farmland natural enemy, contributes to keep ecotope, more friendly to environment.Biopesticide of the present invention is taked directly to extract from Chinese herbal medicine, and production technology is simple, and flow process is short; Greatly reduce production cost, easily industrialization, economic benefit is high.
Indoor antibacterial test
The root of Chinese wild ginger, the bacteriostatic activity test of frutus cnidii ethanol extract to the growth of rotten neck pathogen.Bacteriostatic activity with growth rate method mensuration Chinese herbal medicine extract to confession examination germ.By PDA medium activation for pathogen, cultivate after 5d, punch along colony edge with card punch, form diameter 5.0mm for trying bacterium bacterium cake.Various extracts and PDA medium are mixed with respectively to the band medicine medium of 50mg/mi, pour culture dish into.Dull and stereotyped central authorities move into respectively a diameter 5.0mm for examination bacterium mattress cake, every processing repeats 3 times.At 28 DEG C, cultivate 3d through the above culture dish of processing.With 50% ethanol that adds respective amount in contrast, be placed in 28 DEG C of constant incubators and cultivate 3d, measure colony diameter, calculate the fungistatic effect of each extract.
Fungistatic effect (%)=(contrast colony diameter one is processed colony diameter)/(contrast colony diameter one bacterium cake diameter) × 100%
Meanwhile, compare with 800 times of liquid of 80% carbendazol wettable powder.
Bacteriostatic test proves, the root of Chinese wild ginger, frutus cnidii ethanol extract reach 97.9% to the bacteriostasis of rotten neck pathogen, it contrasts 800 times of liquid bacteriostasis of 80% carbendazol wettable powder is 61.3%, and its bacteriostatic activity is apparently higher than 800 times of liquid of 80% carbendazol wettable powder.
Field trial data
Physical data: test be located at Linyi rice research experimental field in, it is the chronic illness district of rice blast, selected experimental field is semilate rice growing area in flakes, field face is smooth, irrigation and drainage facility, soil, the cultivation of all experimental plots are consistent with rich water quality management level, when the 1st dispenser rotten neck morbidity heavier, disease is in the morbidity epidemic peak phase.
Two processing are established in test altogether: experimental group is used the biopesticide of the present invention 10 times of sprayings that add water.Control group uses rich group (40% Isoprothiolane 100mL/667m
2, Australian Niu Famu company produces, commercially available).Two groups of application methods all adopt foliage spray.Respectively spray once at Rise's boot period, full heading time, spray altogether secondary.Experimental group measures biopesticide 15Kg of the present invention at every turn, and the rich 60-75 gram that sends of every mu of use of control group, is watered 30 kilograms of sprayings.Two groups spray rice area and are 667m
2.In after dispenser 24 hours, meet rainy need to filling spray.
Result of the test is in table 1.
Table 1 experimental group and control group preventive effect are in rate of growth comparison
| Process | Morbidity fringe neck rate (%) | Preventive effect (%) | Rate of growth (%) |
| Experimental group | 21.2 | 80.9 | 9.9 |
| Control group | 19.9 | 59.9 | 6.1 |
| Relatively | Without significant difference | Significant difference | Significant difference |
Evidence biopesticide of the present invention has good result for the treatment of to rice blast, and has significantly improved the output of paddy rice.
Embodiment
Embodiment 1: get the 400g root of Chinese wild ginger, 250g frutus cnidii, pulverize through micropulverizer, cross 100 mesh sieves, add 95% ethanol 550ml, after soaking 6h under room temperature, pour out ethanol, so repeatedly carry out 3 times, merge the leaching liquor of 3 times, obtain the about 230ml of leaching liquor, and then 60 DEG C of water-baths are concentrated into 50ml, for subsequent use after ethanol is volatilized completely.
Embodiment 2: get the 500g root of Chinese wild ginger, 200g frutus cnidii, pulverize through micropulverizer, cross 100 mesh sieves, add 95% ethanol 500ml, after soaking 6h under room temperature, pour out ethanol, so repeatedly carry out 3 times, merge the leaching liquor of 3 times, obtain the about 200ml of leaching liquor, and then 60 DEG C of water-baths are concentrated into 50ml, for subsequent use after ethanol is volatilized completely.
Embodiment 3: get the 300g root of Chinese wild ginger, 300g frutus cnidii, pulverize through micropulverizer, cross 100 mesh sieves, add 95% ethanol 600ml, after soaking 6h under room temperature, pour out ethanol, so repeatedly carry out 3 times, merge the leaching liquor of 3 times, obtain the about 250ml of leaching liquor, and then 60 DEG C of water-baths are concentrated into 50ml, for subsequent use after ethanol is volatilized completely.
Claims (4)
1. prevent and treat a biopesticide for rice disease, it is characterized in that this kind of biopesticide be made up of the Chinese herbal medicine root of Chinese wild ginger, frutus cnidii.
2. a kind of biopesticide of preventing and treating rice disease according to claim 1, is characterized in that this kind of biopesticide is by root of Chinese wild ginger 30-50 part, and frutus cnidii 20-30 part is made.
3. a kind of biopesticide of preventing and treating rice disease according to claim 1, is characterized in that this kind of biopesticide is by 40 parts of the roots of Chinese wild ginger, and 25 parts of frutus cnidiis are made.
4. prevent and treat the preparation method of the biopesticide of rice disease for one kind, it is characterized in that getting the 400g root of Chinese wild ginger, 250g frutus cnidii, pulverizes through micropulverizer, cross 100 mesh sieves, add 95% ethanol 500-600ml, pour out ethanol after soaking 6h under room temperature, so repeatedly carry out 3 times, merge the leaching liquor of 3 times, approximately 200～250ml of leaching liquor, and then 60 DEG C of water-baths are concentrated into 50ml, for subsequent use after ethanol is volatilized completely.
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| CN201410327519.0A CN104082348B (en) | 2014-07-01 | 2014-07-01 | A kind of biopesticide and preparation method preventing and treating rice disease |
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| CN104082348A true CN104082348A (en) | 2014-10-08 |
| CN104082348B CN104082348B (en) | 2016-04-13 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982471A (en) * | 2015-07-15 | 2015-10-21 | 广西宏华生物实业股份有限公司 | A method for controlling rice blast of organic rice |
| CN105272561A (en) * | 2015-10-09 | 2016-01-27 | 桂林劲乐生物化工有限公司 | High efficiency and low toxicity citrus shoot killing element with fertilizer efficiency and preparation method thereof |
| CN106538619A (en) * | 2016-11-08 | 2017-03-29 | 佛山市飞时达新材料科技有限公司 | A kind of biological pesticide and its application process of preventing and treating rice blast |
| CN108409431A (en) * | 2018-04-24 | 2018-08-17 | 贵州贵谷农业股份有限公司 | A kind of special disease prevention composition of rice |
Citations (2)
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| CN1820609A (en) * | 2006-04-05 | 2006-08-23 | 东北农业大学 | Chinese herbal medicine germicide |
| CN103262863A (en) * | 2013-06-06 | 2013-08-28 | 叶彬 | Spray type organic pesticide/bactericide for crop public places as well as preparation and use methods thereof |
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2014
- 2014-07-01 CN CN201410327519.0A patent/CN104082348B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1820609A (en) * | 2006-04-05 | 2006-08-23 | 东北农业大学 | Chinese herbal medicine germicide |
| CN103262863A (en) * | 2013-06-06 | 2013-08-28 | 叶彬 | Spray type organic pesticide/bactericide for crop public places as well as preparation and use methods thereof |
Non-Patent Citations (2)
| Title |
|---|
| 何昆等: "中草药萃取液对植物病原真菌、细菌的抑制作用", 《湖南农业科学》, no. 1, 31 December 2003 (2003-12-31), pages 43 - 45 * |
| 石志琦等: "蛇床子素对植物病原真菌抑制机制的初步研究", 《农药学学报》, vol. 6, no. 4, 31 December 2004 (2004-12-31), pages 28 - 32 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982471A (en) * | 2015-07-15 | 2015-10-21 | 广西宏华生物实业股份有限公司 | A method for controlling rice blast of organic rice |
| CN105272561A (en) * | 2015-10-09 | 2016-01-27 | 桂林劲乐生物化工有限公司 | High efficiency and low toxicity citrus shoot killing element with fertilizer efficiency and preparation method thereof |
| CN106538619A (en) * | 2016-11-08 | 2017-03-29 | 佛山市飞时达新材料科技有限公司 | A kind of biological pesticide and its application process of preventing and treating rice blast |
| CN108409431A (en) * | 2018-04-24 | 2018-08-17 | 贵州贵谷农业股份有限公司 | A kind of special disease prevention composition of rice |
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| CN104082348B (en) | 2016-04-13 |
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