CN104082299B - A kind of method of plant growth regulator for E. dunnii cuttage and seedling culture and E. dunnii cuttage and seedling culture - Google Patents
A kind of method of plant growth regulator for E. dunnii cuttage and seedling culture and E. dunnii cuttage and seedling culture Download PDFInfo
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Abstract
The invention belongs to plant propagation technical field, disclose a kind of method of plant growth regulator for E. dunnii cuttage and seedling culture and E. dunnii cuttage and seedling culture. Plant growth regulator for E. dunnii cuttage and seedling culture provided by the invention comprises the first plant growth regulator and the second plant growth regulator, and the first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L～1.6g/L; The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L～2mg/L. Adopt after the first plant growth regulator provided by the invention picks E. dunnii cuttings, carry out moisturizing with the second plant growth regulator pouring again, realize and utilized plant growth regulator to stimulate continuously E. dunnii to take root, significantly improve the rooting rate of E. dunnii cuttage seeding, the E. dunnii cuttage seeding well developed root system of gained, quality is good, is applicable to batch production and produces E. dunnii cuttage seeding, is more conducive to the breeding of E. dunnii.
Description
Technical field
The invention belongs to plant propagation technical field, particularly a kind of plant growth for E. dunnii cuttage and seedling culture is adjustedThe method of joint agent and E. dunnii cuttage and seedling culture.
Background technology
E. dunnii (EucalyptusdunniiMaiden), Evergreen Broad-leaved Tree Species, high megaphanerophyte, 5 years raw E. dunniisThe diameter of a cross-section of a tree trunk 1.3 meters above the ground can reach 28cm, and the height of tree reaches 17m, and trunk is perfectly straight, flourishing, belongs to two opercules that eucalyptus belongs to (Eucalyptus) in classification(the series of subgenus (subgenussmphyomyrtu) blue gum group (sectionMaidenaria) ribbon gum systemViminales), its original producton location Australia, wildwood distributes in New South Wales,Australia northeast and east, Queensland stateSouth compared with zonule. At present, approximately 800 hectares of the wildwood areas of New South Wales, lazy weight 82000 strains, in QueenslandStill less, therefore, E. dunnii has been divided into endangered species in Australia for the area in state and quantity, its wildwood distribution district quilt mostlyPut national park or protection zone under.
The tree body of E. dunnii is medium bigger than normal, and material is good, and rapidly, cold resistance is stronger in growth, is that one is applicable to China very muchOne of eucalyptus kind of middle subtropical zone area plantation is important gardens and cycle industrial material forest seeds. The breeding side of E. dunnii at presentMethod comprises seed propagation and cuttage and seedling culture breeding, mainly taking seed propagation for example, as main: get E. dunnii thousand grain weigth 2.457g(407000/kg), collected seed annual 10～December, early March sowing, application rate 10～12g/m2, 633 strains of on average emerging/m2, per kilogram approximately 63300 strains of emerging, emergence rate only has an appointment 16%. The limitation of seed propagation is the seed production of E. dunniiSeldom, good provenance is all import, and emergence rate is low, and the seedling crop Growth and Differentiation of gained is larger, cold resistance individual differenceSignificantly, and forest form is irregular, and spread has been caused to restriction.
Cuttage and seedling culture can solve the shortcoming that seed propagation output is little, quality is inhomogeneous to a certain extent. For DunneEucalyptus cuttage and seedling culture, the difficulty of taking root, survival rate is low is the technical bottleneck in this field, and traditional cuttage and seedling culture method is more than 3～May or justSummer harvest collection cuttings, utilizes the spring tip to be beneficial to taking root of E. dunnii, and production is short season, and the root system growing is undeveloped, generally only has, a main root, easily causes that E. dunnii First Year branch bar is thrown oneself on the ground, poor growth phenomenon, affects the quality of gained E. dunnii, and existingReport rests on experimental stage, and batch production is produced and had no report. In order to make full use of E. dunnii resource, be necessary to provide a kind of rawRoot rate is high, well developed root system, quality are good, be applicable to the method for the E. dunnii cuttage and seedling culture of suitability for industrialized production.
Summary of the invention
In view of this, goal of the invention of the present invention is to provide a kind of plant growth regulating for E. dunnii cuttage and seedling cultureThe method of agent and E. dunnii cuttage and seedling culture. Plant growth regulator provided by the invention can promote E. dunnii cuttage rooting, carriesThe high rooting rate of E. dunnii cuttage seeding, gained E. dunnii cuttage seeding well developed root system, quality is good, is applicable to batch production and produces E. dunniiCuttage seeding, is more conducive to the breeding of E. dunnii.
In order to realize goal of the invention of the present invention, the present invention adopts following technical scheme:
The invention provides a kind of plant growth regulator for E. dunnii cuttage and seedling culture, it comprises the first plant growthConditioning agent and the second plant growth regulator;
This first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L～1.6g/L;
This second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L～2mg/L.
The growth that plant growth regulator carrys out regulation and control plant by the Endogenous Hormone Contents in Vitro in regulating plant body is sent outEducate and various physiological activity, the plant growth regulator of auxins has positive and negative duality to the effect of plant, lowerWhen concentration, can promote plant germination, take root, tachyauxesis, but in the time of higher concentration, can suppress again plant growth. This ShenAfter please finding that by a large amount of experiments E. dunnii cuttings is picked to the first plant growth regulator, carry out cuttage, then adopt lower denseThe second plant growth regulator of degree carries out moisturizing, can realize and utilize plant growth regulator to stimulate continuously DunneEucalyptus takes root, and does not produce depression effect, and then has improved the rooting rate of E. dunnii cuttage seeding. Experimental result confirms, adopts firstPlant growth regulator and the second plant growth regulator treatment E. dunnii have significantly improved the rooting rate of E. dunnii cuttage seeding, instituteE. dunnii cuttage seeding well developed root system, quality is good, high, the phenomenon that do not occur throwing oneself on the ground of survival rate, gained Dunne eucalyptus after transplantingShape grace, is applicable to batch production and produces E. dunnii cuttage seeding, is more conducive to the breeding of E. dunnii.
Preferably, in plant growth regulator provided by the invention, indolebutyric acid and naphthalene in the first plant growth regulatorThe mass ratio of acetic acid is 3:2 or 5:3.
Preferably, in plant growth regulator provided by the invention, indolebutyric acid and naphthalene in the second plant growth regulatorThe mass ratio of acetic acid is 3:2 or 5:3.
Preferably, in plant growth regulator provided by the invention, also comprise nutrient solution, it comprises following component:
The present invention also provides a kind of method of utilizing plant growth regulator to carry out E. dunnii cuttage and seedling culture, and this plant is rawLong conditioning agent comprises the first plant growth regulator and the second plant growth regulator;
This first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L～1.6g/L;
This second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L～2mg/L;
The method of this E. dunnii cuttage and seedling culture, comprises the following steps:
Step 1: obtain E. dunnii cuttings;
Step 2: get E. dunnii cuttings and pick after the first plant growth regulator, cuttage is in Nutrition Soil;
Step 3: pouring the second plant growth regulator, cultivate, obtain E. dunnii cuttage seeding;
The frequency of the pouring in step 3 be every 5～8 days once; The number of times of pouring is 4 times～6 times.
Preferably, in the method for E. dunnii cuttage and seedling culture provided by the invention, the first plant growth regulator in step 2The mass ratio of middle indolebutyric acid and methyl α-naphthyl acetate is 3:2 or 5:3.
Preferably, in the method for E. dunnii cuttage and seedling culture provided by the invention, the second plant growth regulator in step 2The mass ratio of middle indolebutyric acid and methyl α-naphthyl acetate is 3:2 or 5:3.
Preferably, in the method for E. dunnii cuttage and seedling culture provided by the invention, in step 1, E. dunnii cuttings is E. dunnii halfLignifying spray, contains 4～6 true leaves, a terminal bud.
Preferably, in the method for E. dunnii cuttage and seedling culture provided by the invention, in step 2, Nutrition Soil is yellow soil and huskThe mixture of ash, in percent by volume, wherein the content of rice husk ash is 20%～40%.
In some embodiments of the invention, in the method for E. dunnii cuttage and seedling culture provided by the invention, used in step 2The Fertility Parameters of yellow soil is: ammonium state N:15～35mg/L, available P: 10～25mg/L, effective K:100～250mg/L.
In other embodiment of the present invention, in the method for E. dunnii cuttage and seedling culture provided by the invention, in step 2The Fertility Parameters of rice husk ash used is: ammonium state N:15～40mg/L, the effective K:500～700mg/L of available P: 10～25mg/L.
In other embodiment of the present invention, in the method for E. dunnii cuttage and seedling culture provided by the invention, in step 2The density of cuttage be 1000～1500 strains/square metre.
Preferably, in the method for E. dunnii cuttage and seedling culture provided by the invention, the time picking in step 2 is 1 second～3Second.
In other embodiment of the present invention, in the method for E. dunnii cuttage and seedling culture provided by the invention, in step 2The position that picks the corresponding E. dunnii cuttings of the first plant growth regulator is: E. dunnii cuttings end 2～5cm, this endFor E. dunnii cuttings lower cut end.
In other embodiment of the present invention, in the method for E. dunnii cuttage and seedling culture provided by the invention, in step 1The lower cut end of E. dunnii cuttings is apart from 2～5 millimeters, a joint nearest in cuttings.
Preferably, in the method for E. dunnii cuttage and seedling culture provided by the invention, the amount of pouring wetting with Nutrition Soil in step 3Degree reaches 75%～95% and is as the criterion.
Preferably, in the method for E. dunnii cuttage and seedling culture provided by the invention, step 2 Nutrition Soil used was used before cuttageNutrient solution pouring is processed;
Nutrient solution wherein comprises following component:
Wherein, watering nutrient solution used amount is every cubic metre of Nutrition Soil pouring 100L～120L nutrient solution, now, and nutritionThe humidity of soil reaches 75%～95%.
The present invention also provides a kind of method of utilizing the extensive E. dunnii of plant growth regulator to grow seedlings, this plant growthConditioning agent comprises the first plant growth regulator and the second plant growth regulator;
This first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L～1.6g/L;
This second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L～2mg/L;
The method that this E. dunnii is grown seedlings, comprises the following steps:
Step 1: obtain E. dunnii cuttings;
Step 2: get E. dunnii cuttings and pick after the first plant growth regulator, cuttage is in Nutrition Soil;
Step 3: pouring the second plant growth regulator, the frequency of pouring be every 5～8 days once, the number of times of pouring is 4 times～6 times;
Step 4: cultivate, in the time that Eucalypts camaldulensis,E. seedlings grows to 10cm～30cm, cut semi-lignified spray, obtain semi-lignifiedSpray, nursery stock;
Step 5: get semi-lignified spray that step 4 obtains as new E. dunnii cuttings, get this new E. dunnii cuttingsRepeating step 2, to step 4, is collected gained nursery stock, obtains E. dunnii cuttage seeding.
In the method that extensive E. dunnii provided by the invention is grown seedlings, step 5 is specially: that gets that step 4 obtains is half woodenChange spray as new E. dunnii cuttings, get this new E. dunnii cuttings repeating step 2 to step 4, until the number of gained nursery stockThe realistic nursery stock number of order demand, collects the nursery stock of repeating each time gained, obtains E. dunnii cuttage seeding.
In some embodiments of the invention, in extensive E. dunnii method for culturing seedlings provided by the invention, the institute in step 4The nursery stock obtaining can be used as maternal plant, after cultivating, can sprout 2～4 side shoots, when collateral generation is to 10cm～30cmTime, cut semi-lignified spray, gained semi-lignified spray, as new E. dunnii cuttings, is got this new E. dunnii cuttings and is repeatedStep 2, to step 4, is prepared new nursery stock; The nursery stock that plucks side shoot stem apex (being semi-lignified spray), can continue as maternal plantRepeat the above-mentioned process of being prepared semi-lignified spray by maternal plant, also can plant trees according to the actual requirements. Collect and utilizeSemi-lignified spray that the side shoot of maternal plant prepares, and the final Eucalypts camaldulensis,E. seedlings obtaining. Finally merge by E. dunnii maternal plantMajor branch top (the being semi-lignified spray) Eucalypts camaldulensis,E. seedlings preparing, the E. dunnii that utilizes the side shoot of maternal plant to prepareNursery stock and the maternal plant of side shoot is provided, obtains E. dunnii cuttage seeding. Utilize the method can further accelerate E. dunnii cuttage seedingProliferative speed. As repeated to obtain altogether 26000 strain E. dunniis from 2000 initial strain E. dunnii cuttings through 4 times in embodiment 8Cuttage seeding, has realized extensive E. dunnii and has grown seedlings.
Preferably, in the method that extensive E. dunnii provided by the invention is grown seedlings, the first plant growth regulating in step 2In agent, the mass ratio of indolebutyric acid and methyl α-naphthyl acetate is 3:2 or 5:3.
Preferably, in the method that extensive E. dunnii provided by the invention is grown seedlings, the second plant growth regulating in step 2In agent, the mass ratio of indolebutyric acid and methyl α-naphthyl acetate is 3:2 or 5:3.
Preferably, in the method that extensive E. dunnii provided by the invention is grown seedlings, in step 1, E. dunnii cuttings is E. dunniiSemi-lignified spray, contains 4～6 true leaves, a terminal bud.
Preferably, in the method that extensive E. dunnii provided by the invention is grown seedlings, in step 2, Nutrition Soil is yellow soil and paddyThe mixture of shell ash, in percent by volume, wherein the content of rice husk ash is 20%～40%.
In some embodiments of the invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, institute in step 2With the Fertility Parameters of yellow soil be: ammonium state N:15～35mg/L; Available P: 10～25mg/L; Effectively K:100～250mg/L.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 2In the Fertility Parameters of rice husk ash used be: ammonium state N:15～40mg/L; Available P: 10～25mg/L; Effectively K:500～700mg/L。
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 2The density of middle cuttage be 1000～1500 strains/square metre.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 2In time of picking be 1 second～3 seconds.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 2In the position that picks be: E. dunnii cuttings end 2cm～5cm, this end is E. dunnii cuttings lower cut end.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 1The lower cut end of middle E. dunnii cuttings is apart from a joint 2cm～5cm nearest in cuttings.
Preferably, in the method that extensive E. dunnii provided by the invention is grown seedlings, in step 3, the amount of pouring is with Nutrition SoilHumidity reaches 75%～95% and is as the criterion.
Preferably, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 2 Nutrition Soil used is before cuttageWith nutrient solution pouring processing;
Nutrient solution wherein comprises following component:
Wherein, watering nutrient solution used amount is every cubic metre of Nutrition Soil pouring 100L～120L nutrient solution, now, and nutritionThe humidity of soil reaches 75%～95%.
Preferably, in the method that extensive E. dunnii provided by the invention is grown seedlings, in step 4, cultivate comprise shade, topdress,Diseases prevention, mildew-resistant, insect protected.
In some embodiments of the invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 4 is shadedBe specially: get sunshade net, on covering and seedbed arched frame.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 4Seedbed arched frame bow member used be the bamboo cane sheet that is across the wide 1.5cm～2.5cm in seedbed, limit, insertion seedbed, bow member two endsEdge, interval 65cm～75cm, the arched frame that the height that forms arched frame apogee distance ground is 45cm～55cm.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 4The fertilizer used that topdresses is: urea+potassium dihydrogen phosphate+ferrous sulfate or potassium dihydrogen phosphate+carbendazim.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 4The disease that diseases prevention prevents and treats comprises: malnutrition, leaf roll, leaf blight, black patches disease.
In other embodiment of the present invention, in the method that extensive E. dunnii provided by the invention is grown seedlings, step 4The worm that insect protected prevents and treats comprises: plant hopper, looper, mealybug.
The invention provides a kind of plant growth regulator for E. dunnii cuttage and seedling culture and E. dunnii cuttage and seedling cultureMethod. Plant growth regulator for E. dunnii cuttage and seedling culture provided by the invention comprises the first plant growth regulator andTwo plant growth regulator, this first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, its concentration be 1g/L～1.6g/L; This second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L～2mg/L. AdoptAfter picking E. dunnii cuttings with the first plant growth regulator provided by the invention, then with the second plant growth regulator pouringCarry out moisturizing, realized and utilized plant growth regulator to stimulate continuously E. dunnii to take root. Realize result and confirm, the present inventionThe plant growth regulator providing has significantly improved the rooting rate of E. dunnii cuttage seeding, and the E. dunnii cuttage seeding root system of gained is sent outReach, quality is good, is applicable to batch production and produces E. dunnii cuttage seeding, is more conducive to the breeding of E. dunnii.
Detailed description of the invention
The invention discloses a kind of plant growth regulator for E. dunnii cuttage and seedling culture and E. dunnii cuttage and seedling cultureMethod. Those skilled in the art can obtain E. dunnii cuttage seeding with reference to this paper Content Implementation the method, of particular noteBe, all similar replacements and change apparent to those skilled in the artly, they are all deemed to be included in thisIn invention. Preparation method of the present invention is described by preferred embodiment, and related personnel obviously can not depart fromIn content of the present invention, spirit and scope, this paper preparation method changed or suitably change and combination, realizing and apply thisInvention technology.
A kind of plant growth regulator for E. dunnii cuttage and seedling culture provided by the invention and E. dunnii cuttage and seedling cultureIn method, reagent used and raw material all can be buied by market.
In order to make those skilled in the art can understand better technical scheme of the present invention, below in conjunction with enforcementExample, further set forth the present invention:
Embodiment 1 utilizes plant growth regulator to carry out E. dunnii cuttage and seedling culture
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.6g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 5:3, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L, wherein indoles fourthThe mass ratio of acid and methyl α-naphthyl acetate is 5:3, the NaOH solution that solvent is 1mmol/L.
Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, and in percent by volume, wherein the content of rice husk ash is20%; Wherein the Fertility Parameters of yellow soil is: ammonium state N:15mg/L, available P: 25mg/L, effective K:100mg/L; Husk ash fertilizerForce parameter: ammonium state N:15mg/L, available P: 10mg/L, effective K:500mg/L. Nutrition Soil is poured into the Plastic Flat of 7cm × 11cmIn pocket, be prepared as nutrient bag, before use, water irrigates.
March, the maternal plant of the E. dunnii choiceness of cultivating from Zu Pei center, botanical garden, Hunan Province is got E. dunniiCuttings, gained E. dunnii cuttings is semi-lignified spray, length is 15cm, every section of 4 true leaves, a terminal bud, lower cut distanceThe distance of first joint is 2mm～5mm, repeats to test three times, gets cuttings totally 20 strains at every turn. Clamp a strain E. dunnii with tweezersThe lower end of cuttings, puts into apart from the position of end 2cm the first plant growth regulator preparing by cuttings bottom and is stained withGet, the time picking is 1s. In the nutrient bag that the E. dunnii cuttings insertion water of gained after picking is irrigated, each nutrient bagMiddle cuttage 1 strain E. dunnii cuttings, cultivates.
After cultivating 7 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 7 days, waters altogether 4 times afterwards, obtains DengGrace eucalyptus cuttage seeding, collects E. dunnii cuttage seeding, and the situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By Dunne of gainedEucalyptus cuttage seeding, after hardening, is transplanted the E. dunnii cuttage seeding of taking root to land for growing field crops, continues to cultivate June to investigate E. dunnii growthSituation, in table 1.
Embodiment 2 utilizes plant growth regulator to carry out E. dunnii cuttage and seedling culture
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 2mg/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mmol/L.
Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, and in percent by volume, wherein the content of rice husk ash is40%; Wherein the Fertility Parameters of yellow soil is: ammonium state N:35mg/L, available P: 10mg/L, effective K:250mg/L; Husk ash fertilizerForce parameter: ammonium state N:40mg/L, available P: 25mg/L, effective K:700mg/L. Nutrition Soil is poured into the Plastic Flat of 7cm × 11cmIn pocket, be prepared as nutrient bag, before use, water irrigates.
May, the E. dunnii maternal plant of the E. dunnii choiceness of cultivating from Zu Pei center, botanical garden, Hunan Province is gotE. dunnii cuttings, gained E. dunnii cuttings is semi-lignified spray, length is 20cm, every section of 6 true leaves, a terminal bud, incisionMouth is 2mm～5mm apart from the distance of first joint, repeats to test three times, gets cuttings totally 20 strains at every turn. Clamp a strain with tweezersThe lower end of E. dunnii cuttings, puts into cuttings bottom the first plant growth regulator preparing apart from the position of end 5cmPick, the time picking is 3s. In the nutrient bag that the E. dunnii cuttings insertion water of gained after picking is irrigated, eachIn nutrient bag, cuttage 1 strain E. dunnii cuttings, cultivates.
After cultivating 5 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 5 days, waters altogether 6 times afterwards, obtains DengGrace eucalyptus cuttage seeding, collects E. dunnii cuttage seeding, and the situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By Dunne of gainedEucalyptus cuttage seeding, after hardening, is transplanted the E. dunnii cuttage seeding of taking root to land for growing field crops, continues to cultivate June to investigate E. dunnii growthSituation, in table 1.
Embodiment 3 utilizes plant growth regulator to carry out E. dunnii cuttage and seedling culture
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.2g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 3:2, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.5mg/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 3:2, the NaOH solution that solvent is 1mmol/L.
Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, and in percent by volume, wherein the content of rice husk ash is30%; Wherein the Fertility Parameters of yellow soil is: ammonium state N:30mg/L, available P: 15mg/L, effective K:200mg/L; Husk ash fertilizerForce parameter: ammonium state N:30mg/L, available P: 20mg/L, effective K:600mg/L. Nutrition Soil is poured into the Plastic Flat of 7cm × 11cmIn pocket, be prepared as nutrient bag, before use, water irrigates.
September, the E. dunnii maternal plant of the E. dunnii choiceness of cultivating from Zu Pei center, botanical garden, Hunan Province is gotE. dunnii cuttings, gained E. dunnii cuttings is semi-lignified spray, is semi-lignified spray, and length is 18cm, and 5 every section are trueLeaf, a terminal bud, lower cut is 2mm～5mm apart from the distance of first joint, repeats to test three times, gets cuttings totally 20 at every turnStrain. Clamp the lower end of a strain E. dunnii cuttings with tweezers, by cuttings bottom apart from the position of end 3cm put into prepare firstIn plant growth regulator, pick, the time picking is 2s. The E. dunnii cuttings of gained after picking is inserted to water watersIn saturating nutrient bag, in each nutrient bag, cuttage 1 strain E. dunnii cuttings, cultivates.
After cultivating 8 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 8 days, waters altogether 5 times afterwards, obtains DengGrace eucalyptus cuttage seeding, collects E. dunnii cuttage seeding, and the situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By Dunne of gainedEucalyptus cuttage seeding, after hardening, is transplanted the E. dunnii cuttage seeding of taking root to land for growing field crops, continues to cultivate June to investigate E. dunnii growthSituation, in table 1.
Embodiment 4 utilizes plant growth regulator to carry out E. dunnii cuttage and seedling culture
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.2g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 5:3, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.5mg/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 3:2, the NaOH solution that solvent is 1mmol/L.
Nutrient solution: take NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、H3BO3、ZnSO4·7H2O、Na2MoO4·2H2O、MnSO4·4H2O、Na2EDTA、FeSO4·7H2O、CuSO4·5H2O、CoCl2·6H2O, is dissolved inWater, is mixed with nutrient solution, and in gained nutrient solution, the concentration of each material is: NH4NO3180mg/L、KNO3200mg/L、KH2PO420mg/L、CaCl2·2H2O50mg/L、MgSO4·7H2O41mg/L、KI0.1mg/L、H3BO30.6mg/L、ZnSO4·7H2O0.9mg/L、Na2MoO4·2H2O0.02mg/L、MnSO40.2mg/L、Na2EDTA9mg/L、FeSO4·7H2O6mg/L、CuSO4·5H2O0.2μg/L、CoCl2·6H2O2μg/L。
Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, and in percent by volume, wherein the content of rice husk ash is30%; Wherein the Fertility Parameters of yellow soil is: ammonium state N:30mg/L, available P: 15mg/L, effective K:200mg/L; Husk ash fertilizerForce parameter: ammonium state N:30mg/L, available P: 20mg/L, effective K:600mg/L. Nutrition Soil is poured into the Plastic Flat of 7cm × 11cmIn pocket, be prepared as nutrient bag, before use, irrigate with nutrient solution, every cubic metre of Nutrition Soil pouring 120L nutrient solution, Nutrition SoilHumidity reach 75%-95%.
March, the E. dunnii maternal plant of the E. dunnii choiceness of cultivating from Zu Pei center, botanical garden, Hunan Province is gotE. dunnii cuttings, gained E. dunnii cuttings is semi-lignified spray, length is 15cm, every section of 4 true leaves, a terminal bud, incisionMouth is 2mm～5mm apart from the distance of first joint, repeats to test three times, gets cuttings totally 20 strains at every turn. Clamp a strain with tweezersThe lower end of E. dunnii cuttings, puts into cuttings bottom the first plant growth regulator preparing apart from the position of end 2cmPick, the time picking is 2s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution,In each nutrient bag, cuttage 1 strain E. dunnii cuttings, cultivates.
After cultivating 5 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 5 days, waters altogether 6 times afterwards, obtains DengGrace eucalyptus cuttage seeding, collects E. dunnii cuttage seeding, and the situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By Dunne of gainedEucalyptus cuttage seeding, after hardening, is transplanted the E. dunnii cuttage seeding of taking root to land for growing field crops, continues to cultivate June to investigate E. dunnii growthSituation, in table 1. Embodiment 5 utilizes plant growth regulator to carry out E. dunnii cuttage and seedling culture
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 5:3, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 2mg/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 5:3, the NaOH solution that solvent is 1mmol/L.
Nutrient solution: take NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、H3BO3、ZnSO4·7H2O、Na2MoO4·2H2O、MnSO4·H2O、Na2EDTA、FeSO4·7H2O、CuSO4·5H2O、CoCl2·6H2O, water-soluble,Be mixed with nutrient solution, in gained nutrient solution, the concentration of each material is: NH4NO3220mg/L、KNO3250mg/L、KH2PO424mg/L、CaCl2·2H2O60mg/L、MgSO4·7H2O50mg/L、KI0.9mg/L、H3BO30.9mg/L、ZnSO4·7H2O1.2mg/L、Na2MoO4·2H2O0.04mg/L、MnSO43.7mg/L、Na2EDTA11mg/L、FeSO4·7H2O8mg/L、CuSO4·5H2O0.4μg/L、CoCl2·6H2O4μg/L。
Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, and in percent by volume, wherein the content of rice husk ash is20%; Wherein the Fertility Parameters of yellow soil is: ammonium state N:35mg/L, available P: 25mg/L, effective K:250mg/L; Husk ash fertilizerForce parameter: ammonium state N:40mg/L, available P: 25mg/L, effective K:700mg/L. Nutrition Soil is poured into the Plastic Flat of 7cm × 11cmIn pocket, be prepared as nutrient bag, before use, irrigate with nutrient solution, every cubic metre of Nutrition Soil pouring 100L nutrient solution, makes nutritionThe humidity of soil reaches 75%-95%.
E. dunnii cuttings comes from the E. dunnii mother of the E. dunnii choiceness of cultivating at Zu Pei center, botanical garden, Hunan ProvinceStrain, is semi-lignified spray, and length is 20cm, every section of 6 true leaves, a terminal bud, and lower cut apart from the distance of first joint is2mm～5mm, repeats to test three times, gets cuttings totally 20 strains at every turn. Clamp the lower end of a strain E. dunnii cuttings with tweezers, will insertFringe bottom is put into apart from the position of end 5cm the first plant growth regulator preparing and is picked, and the time picking is1s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 strain Deng in each nutrient bagGrace eucalyptus cuttings, cultivates.
After cultivating 6 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 6 days, waters altogether 5 times afterwards, obtains DengGrace eucalyptus cuttage seeding, collects E. dunnii cuttage seeding, and the situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By Dunne of gainedEucalyptus cuttage seeding, after hardening, is transplanted the E. dunnii cuttage seeding of taking root to land for growing field crops, continues to cultivate June to investigate E. dunnii growthSituation, in table 1.
Embodiment 6 utilizes plant growth regulator to carry out E. dunnii cuttage and seedling culture
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.6g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 3:2, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L, wherein indoles fourthThe mass ratio of acid and methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mmol/L.
Nutrient solution: take NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、H3BO3、ZnSO4·7H2O、Na2MoO4·2H2O、MnSO4·H2O、Na2EDTA、FeSO4·7H2O、CuSO4·5H2O、CoCl2·6H2O, water-soluble,Be mixed with nutrient solution, in gained nutrient solution, the concentration of each material is: NH4NO3200mg/L、KNO3220mg/L、KH2PO422mg/L、CaCl2·2H2O55mg/L、MgSO4·7H2O45mg/L、KI0.5mg/L、H3BO30.8mg/L、ZnSO4·7H2O1.0mg/L、Na2MoO4·2H2O0.03mg/L、MnSO4·4H2O2.0mg/L、Na2EDTA10mg/L、FeSO4·7H2O7mg/L、CuSO4·5H2O0.3μg/L、CoCl2·6H2O3μg/L。
Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, and in percent by volume, wherein the content of rice husk ash is40%; Wherein the Fertility Parameters of yellow soil is: ammonium state N:15mg/L, available P: 10mg/L, effective K:100mg/L; Husk ash fertilizerForce parameter: ammonium state N:15mg/L, available P: 10mg/L, effective K:500mg/L. Nutrition Soil is poured into the Plastic Flat of 7cm × 11cmIn pocket, be prepared as nutrient bag, before use, irrigate with nutrient solution, every cubic metre of Nutrition Soil pouring 110L nutrient solution, makes nutritionThe humidity of soil reaches 75%-95%.
It is E. dunnii mother that E. dunnii cuttings comes from the E. dunnii choiceness of cultivating at Zu Pei center, botanical garden, Hunan ProvinceStrain, is semi-lignified spray, and length is 18cm, every section of 5 true leaves, a terminal bud, and lower cut apart from the distance of first joint is2mm～5mm, repeats to test three times, gets cuttings totally 20 strains at every turn. Clamp the lower end of a strain E. dunnii cuttings with tweezers, will insertFringe bottom is put into apart from the position of end 4cm the first plant growth regulator preparing and is picked, and the time picking is3s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 strain Deng in each nutrient bagGrace eucalyptus cuttings, cultivates.
After cultivating 8 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 8 days, waters altogether 4 times afterwards, obtains DengGrace eucalyptus cuttage seeding, collects E. dunnii cuttage seeding, and the situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By Dunne of gainedEucalyptus cuttage seeding, after hardening, is transplanted the E. dunnii cuttage seeding of taking root to land for growing field crops, continues to cultivate June to investigate E. dunnii growthSituation, in table 1.
Embodiment 7 adopts the quality evaluation of the E. dunnii cuttage seeding of cuttage and seedling culture method acquisition provided by the invention
E. dunnii survival rate, volt from the quantity of taking root of E. dunnii cuttage seeding, main root quantity, root length, rooting rate, transplantingThe method of the E. dunnii cuttage and seedling culture that the embodiment of the present invention 1 to embodiment 6 provides is investigated in the several aspects of quantity of ground E. dunniiThe quality of the E. dunnii cuttage and seedling culture preparing, prepares E. dunnii cuttage and seedling culture as a control group 1, right with the following method to adoptAccording to group 2, control group 3, control group 4, control group 5; Blank group is not used plant growth regulator.
Control group 1 plant growth regulator used is ABT2 root-inducing powder+talcum powder+carbendazim, and concentration is 10mg/L,Solvent is the NaOH solution of 1mol/L. Control group 2, control group 3 the first plant growth used are adjusted, the second plant growth regulatorIdentical: the first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, its concentration is 1g/L; The second plant growth regulatorBe made up of indolebutyric acid and methyl α-naphthyl acetate, its concentration is 0.83mg/L, the NaOH solution that solvent is 1mol/L, indolebutyric acid and naphthalene secondThe ratio of acid is 3:2. Control group 4 plant growth regulator used is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.8g/L,The ratio of indolebutyric acid and methyl α-naphthyl acetate is 3:2. Control group 5 plant growth regulator used is indolebutyric acid, and its concentration is600mg/L。
It is good that five control group E. dunnii cuttings used all come from the E. dunnii of cultivating at Zu Pei center, botanical garden, Hunan ProvinceClonal maternal plant, it is semi-lignified spray, length is 20cm, every section of 5 true leaves, a terminal bud, lower cut distance firstThe distance of individual joint is 2mm～5mm, is divided at random five groups, and every group of 20 strains, for cuttage. Nutrition Soil used is yellow soil and rice husk ashHomogeneous mixture, in percent by volume, wherein the content of rice husk ash is 20%, wherein the Fertility Parameters of yellow soil is: ammoniumState N:15mg/L, available P: 25mg/L, effective K:100mg/L; Rice husk ash Fertility Parameters: ammonium state N:15mg/L, available P: 10mg/L, effective K:500mg/L. Nutrition Soil is poured in the Plastic Flat pocket of 7cm × 11cm, be prepared as nutrient bag, before use, useWater irrigates.
Control group 1 cuttage and seedling culture method: get E. dunnii cuttings, clamp the lower end of a strain E. dunnii cuttings with tweezers, will insertThe plant growth regulator ABT2 root-inducing powder+talcum powder+carbendazim preparing is put in fringe bottom apart from the position of end 4cm,Concentration is to soak in 10mg/L, and the time of immersion is 30m. By the E. dunnii cuttings insertion nutrient solution of gained after soakingIn the nutrient bag irrigating, in each nutrient bag, cuttage 1 strain E. dunnii cuttings, cultivates. Watered afterwards a water every 7 days,Keeping the humidity of the Nutrition Soil in nutrient bag is 75%～95%, waters altogether 4 times. Obtain E. dunnii cuttage seeding, collect DunneEucalyptus cuttage seeding, the situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By the E. dunnii cuttage seeding of gained after hardening,The E. dunnii cuttage seeding of taking root is transplanted to land for growing field crops, continue to cultivate June to investigate the growing state of E. dunnii, in table 1.
The cottage method of control group 2 is: get E. dunnii cuttings, clamp the lower end of a strain E. dunnii cuttings with tweezers, will insertFringe bottom is put into the first plant growth regulator preparing and is picked, and the E. dunnii cuttings of gained after picking is insertedIn nutrient bag, cultivate. After cultivating 7 days, watering pours water carries out moisturizing, every a water of pouring in 7 days, waters altogether 6 afterwardsInferior, to obtain final product. The situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By the E. dunnii cuttage seeding of gained after hardening,The E. dunnii cuttage seeding of taking root is transplanted to land for growing field crops, continue to cultivate June to investigate the growing state of E. dunnii, in table 1.
The cottage method of control group 3 is: get E. dunnii cuttings, clamp the lower end of a strain E. dunnii cuttings with tweezers, will insertFringe bottom is put into water and is picked, and the E. dunnii cuttings of gained after picking is inserted in nutrient bag, cultivates. Cultivate 7After it, pouring the second plant growth regulator solution carries out moisturizing, afterwards every second plant growth regulating of pouring in 7 daysAgent solution, waters altogether 6 times, to obtain final product. The situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By the E. dunnii skewer of gainedSeedlings plugging, after hardening, is transplanted the E. dunnii cuttage seeding of taking root to land for growing field crops, continues to cultivate June to investigate the growth feelings of E. dunniiCondition, in table 1.
The cuttage and seedling culture method of control group 4 is: gets E. dunnii cuttings, clamps the lower end of a strain E. dunnii cuttings with tweezers,The plant growth regulator preparing is put into in cuttings bottom and pick, the E. dunnii cuttings of gained after picking is insertedIn nutrient bag, cultivate. After cultivating 7 days, watering pours water carries out moisturizing, every a water of pouring in 7 days, waters altogether 6 afterwardsInferior, to obtain final product. The situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. By the E. dunnii cuttage seeding of gained after hardening,The E. dunnii cuttage seeding of taking root is transplanted to land for growing field crops, continue to cultivate June to investigate the growing state of E. dunnii, in table 1.
The cuttage and seedling culture method of control group 5 is: gets E. dunnii cuttings, clamps the lower end of a strain E. dunnii cuttings with tweezers,The indolebutyric acid preparing is put into in cuttings bottom and pick, the E. dunnii cuttings of gained after picking is inserted to nutrient bagIn, cultivate. After cultivating 7 days, watering pours water carries out moisturizing, every a water of pouring in 7 days, waters altogether 6 times, afterwards. The situation of taking root of statistics gained E. dunnii cuttage seeding, in table 1. The E. dunnii cuttage seeding of gained, after hardening, will be taken rootE. dunnii cuttage seeding transplant to land for growing field crops, continue to cultivate June to investigate the growing state of E. dunnii, in table 1.
The quality testing of the E. dunnii cuttage seeding that embodiment 1 to embodiment 6, control group 1 to control group 5 obtain the results are shown in Table1。
Each group of table 1 is cultivated the testing result of the E. dunnii cuttage seeding obtaining
As can be known from Table 1, for rooting rate, the rooting rate class of the E. dunnii cuttage seeding that embodiment 1 to embodiment 6 obtainsSeemingly, not remarkable (P > 0.05) of difference; Compared with control group, the taking root of the E. dunnii cuttage seeding that embodiment 1 to embodiment 6 obtainsRate significantly improves, significant difference (P < 0.05); Illustrate that cuttage and seedling culture method provided by the invention has significantly improved E. dunnii cuttageThe rooting rate of seedling. Compare with control group, the quantity of taking root, the main root number of the E. dunnii cuttage seeding that embodiment 1 to embodiment 3 obtainsAmount all significantly increases, significant difference (P < 0.05); Root length also increases substantially, significant difference (P < 0.05); Embodiment 1 is describedThe well developed root system of the cuttage and seedling culture method gained E. dunnii cuttage seeding providing to embodiment 3, has improved the matter of E. dunnii cuttage seedingAmount.
The E. dunnii cuttage seeding that comparative example 1 to embodiment 3 obtains, the E. dunnii skewer that embodiment 4 to embodiment 6 obtainsThe quantity of taking root, main root quantity and the root of seedlings plugging are long, and the root system of the E. dunnii cuttage seeding that discovery embodiment 4 to embodiment 6 obtains obtainsTo further improving, root system is more flourishing, and significant difference (P < 0.05) illustrates that irrigating Nutrition Soil with nutrient solution is E. dunnii cuttageSeedling rooting, growth provide abundant trace element, are more conducive to taking root, growing of cuttage seeding.
For survival rate and the growth conditions of E. dunnii after transplanting, compare with control group, embodiment 1 to embodiment 6 carriesThe cuttage and seedling culture method of confession has significantly improved the survival rate of E. dunnii, significant difference (P < 0.05); And embodiment 1 is to enforcementThere is not the phenomenon of throwing oneself on the ground in the cuttage seeding that example 6 provides, and control group has part E. dunnii cuttage seeding to produce the phenomenon of throwing oneself on the ground.
Contrast control group 1, embodiment 1 to embodiment 6, illustrate compared with existing E. dunnii cuttage and seedling culture method this ShenCuttage and seedling culture method please can significantly improve rooting rate, the root system situation of E. dunnii, improves the matter of gained E. dunnii cuttage seedingAmount.
Contrast control group 2, control group 3, embodiment 1 to embodiment 6 only illustrate and are adopting the first plant growth regulatorAfter speed is stained with and is processed E. dunnii cuttings, then carry out moisturizing with the second plant growth regulator, could effectively improve the life of E. dunniiRoot rate, improve root system, improve the quality of gained E. dunnii cuttage seeding.
Contrast control group 4, control group 5, the excessive concentration of plant growth regulator is described, or too low, all can not be effectiveImprove the rooting rate of E. dunnii.
In sum, the cuttage and seedling culture method that the embodiment of the present invention 1 to embodiment 6 provides has significantly improved E. dunnii and has taken rootRate, gained E. dunnii cuttage seeding well developed root system; Transplant after, E. dunnii sapling survival rate is high, well-grown, do not occur voltGround phenomenon, the tree-like grace of gained E. dunnii, be more conducive to the breeding of E. dunnii.
Embodiment 8 utilizes plant growth regulator to carry out extensive E. dunnii and grows seedlings
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 5:3, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 2mg/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mmol/L.
Build steel structural plastic booth: select on the sunny side, wind sheltering, dry, draining is good, there is no the ground of soil infection venereal disease evilSide's scaffolding, selects strongly professional troop to design and build, area 2000m2Left and right, longitudinally 32m, laterally 65m, pre-buried goodPouring water pipeline, leveling booth ground, cement processed.
Preparing nursery bed: require longitudinally to make bed, long 15m, wide 1.2m, high 15cm, stays the aisle of 30cm between bed, can surrounding lay bricksSurround or prefabricated cross section is the armored concrete of right-angled trapezium (upper base × going to the bottom × height=5cm × 8cm × 15cm), long 200cmWorkpiece surrounds, and seedbed is padded with the cobblestone that 3cm is thick (φ 20mm～40mm) after carrying out, in order to draining.
Prepare nutrient bag: Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, in percent by volume, rice husk ash is usedAmount is 20%, wherein the Fertility Parameters of yellow soil in Nutrition Soil: ammonium state N:15mg/L, available P: 10mg/L, effective K:100mg/L; Rice husk ash Fertility Parameters: ammonium state N:40mg/L, available P: 25mg/L, effective K:700mg/L. By Nutrition Soil fill with in 7cm ×In the Plastic Flat pocket of 11cm, then put in seedbed water and irrigate, for subsequent use.
Utilize the first plant growth regulator and the second growth regulator to process E. dunnii cuttings, prepare altogether 26000Strain E. dunnii cuttage seeding.
Cuttage and seedling culture operation:
Collect E. dunnii cuttings March, it comes from the good nothing of E. dunnii that cultivate at Zu Pei center, botanical garden, Hunan ProvinceThe E. dunnii maternal plant of property system, is semi-lignified spray, and length is 20cm, every section of 6 true leaves, a terminal bud, lower cut distance theThe distance of a joint is 2mm～5mm, gets cuttings totally 2000 strains, for cuttage. Clamp the lower end of a strain E. dunnii cuttings with tweezers,The first plant growth regulator preparing is put into apart from the position of end 2cm in cuttings bottom and picks, pick timeBetween be 1s. In the nutrient bag that the E. dunnii cuttings insertion water of gained after picking is irrigated, cuttage 1 strain Deng in each nutrient bagGrace eucalyptus cuttings, cutting density is 1000 strains/m2, cultivate.
In incubation, should be noted that and shade, be specially: be covered in the little arch in seedbed with the shade net that light transmittance is 5%On frame, little bow member is the bamboo cane sheet that is across the wide 2cm left and right in seedbed, its edge, insertion seedbed, two ends, and interval 70cm left and right,The arched frame of high 50cm left and right in the middle of forming.
After cultivating 7 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 7 days, waters altogether 5 times afterwards, checks DengThe situation of taking root of grace eucalyptus cuttings, finds that E. dunnii cuttings takes root, topdress afterwards, diseases prevention, mildew-resistant, insect protected management so thatEucalypts camaldulensis,E. seedlings Fast Growth.
The fertilizer used that topdresses is every liter of 3g urea, 1g potassium dihydrogen phosphate, 0.3g ferrous sulfate, and fertilizing method is blade face sprayExecute.
Diseases prevention, fungus-proof measure are as follows:
A), because malnutrition causes trunk, each side shoot base portion and bottom leaf Huang, use fertilizer for spraying on leaf surface and plant nutrition liquidSupply with root and branches and leaves nutrient, reduce the nutrient consumption of tree body; B) leaf roll, spraying increases humidity, suitably shades; C) leaf blight is anti-Control, first will avoid insect pest or environment to be not suitable for caused wound, reduce artificially friction or the collision for plant; Summer is highWen Shi, suitably shades to reduce temperature; The relative air humidity of controlling well also sprays bactericidal agent for preventing and treating in time, with 25% carbendazim800 times of liquid spray, continuous spraying 2 times in 15 days, and upper and lower position sprays; D) control of black patches disease, notes strengthening branch shaping and repaiiesCut, the logical light that ventilates, when young leaves launches, sprays 1000 times of liquid of carbendazim, and 10 days spray one-time continuous 3 times are removed with pathogeny in timeBranches and leaves.
Insect protected measure is as follows:
A) plant hopper: by artificial control, strengthen pruning, rationally control branch number, reduce the insect place of hiding; In conjunction with shapingPrune, cut off band worm's ovum branch and unnecessary assorted branch, prevent the overstocked concealment of branches and leaves, be beneficial to ventilation and penetrating light; Or use 10% Imidacloprid500 times of liquid spray; B) looper: the 1000 times of liquid foliage-sprays of cypermethrin with 15%, or metrifonate sprays; C) mealybug: preventionMeasure, notes ventilating, and dark moist place easily grows insect pest, prunes in time overstocked branch; Medical treatment, use a red-spotted lizard mustControl.
After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtain 2000 strain E. dunnii cuttings all take root,Growth conditions is good, and the average plant height of Eucalypts camaldulensis,E. seedlings is 30cm. Cut respectively the semi-lignified spray of these nursery stocks, gained halfLignifying spray length is 10cm-15cm, every section of 4 true leaves, a terminal bud, and lower cut is 2mm apart from the distance of first joint～5mm, obtains cuttings totally 2000 strains, carries out cuttage as new E. dunnii cuttings. Clamp the new E. dunnii cuttings of a strain with tweezersThe lower end of (the 2000 strain major branches that are gained are top), puts into apart from the position of end 2cm first planting of preparing by cuttings bottomIn thing growth regulator, pick, the time picking is 1s. The E. dunnii cuttings of gained after picking is inserted to water to be irrigatedNutrient bag in, cuttage 1 strain E. dunnii cuttings in each nutrient bag, cutting density is 1000 strains/m2, cultivate, cultivatingIn process, should be noted that and shade. After cultivating 7 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep battalionThe humidity of supporting the Nutrition Soil in bag is 75%～95%, every second plant growth regulator of pouring in 7 days, waters altogether afterwardsFill with 5 times, check the situation of taking root of E. dunnii cuttings, find that E. dunnii cuttings takes root, topdress afterwards, diseases prevention, mildew-resistant,Insect protected management is so that Eucalypts camaldulensis,E. seedlings Fast Growth. After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state,100% new E. dunnii cuttings is taken root, growth conditions is good, and the average plant height of gained Eucalypts camaldulensis,E. seedlings is 30cm, cuts halfLignifying spray, this semi-lignified spray length is 9cm, every section of 4 true leaves, a terminal bud, lower cut is apart from first jointDistance is 2mm～5mm, obtains cuttings totally 2000 strains.
Meanwhile, the Eucalypts camaldulensis,E. seedlings that cuttage gained nursery stock is cut gained after semi-lignified spray is for the first time as new DunneEucalyptus maternal plant, continues to cultivate 60 days, and this maternal plant sprouts 2～4 side shoots, and collateral generation is to 10cm～30cm, cuts half woodenChange spray, gained semi-lignified spray is as new E. dunnii cuttings, and the 4000 strain E. dunnii cuttings that are total to newly, as new DengGrace eucalyptus cuttings is carried out cuttage.
Merge gained 2000 strains and 4000 strain E. dunnii cuttings, obtain the new E. dunnii cuttings of 6000 strains, according to identical sideMethod is carried out cuttage, cultivation, after 60 days, obtains 95% new E. dunnii cuttings, 5700 seedlings of having taken root to obtain. Cut semi-lignified tenderThe Eucalypts camaldulensis,E. seedlings of branch continues to cultivate 30 days-60 days as new E. dunnii maternal plant, sprouts 2～4 side shoots, for providingNew E. dunnii cuttings. So repeat 4 times, collect all Eucalypts camaldulensis,E. seedlingses, obtain 26000 strain E. dunnii cuttage seeding, the whole cycleNeed 150 days.
The E. dunnii cuttage seeding of gained, after hardening, is transplanted to land for growing field crops the E. dunnii cuttage seeding of taking root for makingWoods, according to normal forest management method management E. dunnii, and records the growing state of E. dunnii growing state, overall seedling woods,In table 2.
Embodiment 9 utilizes plant growth regulator to carry out E. dunnii and grows seedlings
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.6g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 3:2, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L, wherein indoles fourthThe mass ratio of acid and methyl α-naphthyl acetate is 5:3, the NaOH solution that solvent is 1mmol/L.
Build steel structural plastic booth: the method for building steel structural plastic booth providing with embodiment 8 is identical.
Preparing nursery bed: the method for the preparing nursery bed providing with embodiment 8 is identical.
Prepare nutrient bag: Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, in percent by volume, rice husk ash is usedAmount is 40%, wherein the Fertility Parameters of yellow soil in Nutrition Soil: ammonium state N:35mg/L, available P: 25mg/L, effective K:250mg/L; Rice husk ash Fertility Parameters: ammonium state N:15mg/L, available P: 10mg/L, effective K:500mg/L. By Nutrition Soil fill with in 7cm ×In the Plastic Flat pocket of 11cm, then put in seedbed water and irrigate, for subsequent use.
Utilize the first plant growth regulator and the second growth regulator to process E. dunnii cuttings, prepare altogether 13000Strain E. dunnii cuttage seeding.
Cuttage and seedling culture operation:
Collect E. dunnii cuttings May, it comes from the good nothing of E. dunnii that cultivate at Zu Pei center, botanical garden, Hunan ProvinceThe E. dunnii maternal plant of property system, is semi-lignified spray, and length is 15cm, every section of 4 true leaves, a terminal bud, lower cut distance theThe distance of a joint is 2mm～5mm, gets cuttings totally 1000 strains, for cuttage. Clamp the lower end of a strain E. dunnii cuttings with tweezers,The first plant growth regulator preparing is put into apart from the position of end 2cm in cuttings bottom and picks, pick timeBetween be 3s. In the nutrient bag that the E. dunnii cuttings insertion water of gained after picking is irrigated, cuttage 1 strain Deng in each nutrient bagGrace eucalyptus cuttings, cutting density is 1500 strains/m2, cultivate.
In incubation, should be noted that and shade, be specially: be covered in the little arch in seedbed with the shade net that light transmittance is 5%On frame, little bow member is the bamboo cane sheet that is across the wide 2cm left and right in seedbed, its edge, insertion seedbed, two ends, and interval 70cm left and right,The arched frame of high 50cm left and right in the middle of forming.
After cultivating 5 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 70%～95%, every second plant growth regulator of pouring in 5 days, waters altogether 6 times afterwards, checks DengThe situation of taking root of grace eucalyptus cuttings, finds that E. dunnii cuttings takes root, topdress afterwards, diseases prevention, mildew-resistant, insect protected management so thatEucalypts camaldulensis,E. seedlings Fast Growth.
The fertilizer used that topdresses is potassium dihydrogen phosphate 0.3%+ carbendazim 0.1%. Fertilizing method is foliage-spray.
Diseases prevention, fungus-proof measure are as follows:
A), because malnutrition causes trunk, each side shoot base portion and bottom leaf Huang, use fertilizer for spraying on leaf surface and plant nutrition liquidSupply with root and branches and leaves nutrient, reduce the nutrient consumption of tree body; B) leaf roll, spraying increases humidity, suitably shades; C) leaf blight is anti-Control, first will avoid insect pest or environment to be not suitable for caused wound, reduce artificially friction or the collision for plant; Summer is highWen Shi, suitably shades to reduce temperature; The relative air humidity of controlling well also sprays bactericidal agent for preventing and treating in time, with 25% carbendazim600 times of liquid spray, continuous spraying 3 times in 20 days, and upper and lower position sprays; D) control of black patches disease, notes strengthening branch shaping and repaiiesCut, the logical light that ventilates, when young leaves launches, sprays 500 times of liquid of carbendazim, and 10 days spray one-time continuous 3 times, remove the branch with pathogeny in timeLeaf.
Insect protected measure is as follows:
A) plant hopper: by artificial control, strengthen pruning, rationally control branch number, reduce the insect place of hiding; In conjunction with shapingPrune, cut off band worm's ovum branch and unnecessary assorted branch, prevent the overstocked concealment of branches and leaves, be beneficial to ventilation and penetrating light; Or use 10% Imidacloprid500 times of liquid spray; B) looper: the 1000 times of liquid foliage-sprays of cypermethrin with 15%, or metrifonate sprays; C) mealybug: preventionMeasure, notes ventilating, and dark moist place easily grows insect pest, prunes in time overstocked branch; Medical treatment, use a red-spotted lizard mustControl.
After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 95% E. dunnii cuttings and all takes root, gives birth toLong status is good, and the average plant height of Eucalypts camaldulensis,E. seedlings is 20cm. Cut respectively the semi-lignified spray of these nursery stocks, gained half woodMaterialization spray length is 10cm, every section of 5 true leaves, a terminal bud, and lower cut is 2mm～5mm apart from the distance of first joint,Cuttings is totally 950 strains, carries out cuttage as new E. dunnii cuttings. Clamp the lower end of the new E. dunnii cuttings of a strain with tweezers, willCuttings bottom is put into apart from the position of end 5cm the first plant growth regulator preparing and is picked, the time pickingFor 3s. In the nutrient bag that the E. dunnii cuttings insertion water of gained after picking is irrigated, cuttage 1 strain Dunne in each nutrient bagEucalyptus cuttings, cutting density is 1500 strains/m2, cultivate, in incubation, should be noted that and shade. After cultivating 5 days, useThe second plant growth regulator pouring Nutrition Soil carries out moisturizing, and keeping the humidity of the Nutrition Soil in nutrient bag is 75%～95%,Every second plant growth regulator of pouring in 5 days, water altogether 6 times afterwards, check the situation of taking root of E. dunnii cuttings, send outExisting E. dunnii cuttings is taken root, and topdresses afterwards, diseases prevention, mildew-resistant, insect protected management be so that Eucalypts camaldulensis,E. seedlings Fast Growth. WarpAfter the cultivation of spending 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains that 100% new E. dunnii cuttings is taken root, growth conditionsWell, the average plant height of gained Eucalypts camaldulensis,E. seedlings is 20cm, cuts semi-lignified spray, and this semi-lignified spray length is10cm, every section of 6 true leaves, a terminal bud, lower cut is 2mm～5mm apart from the distance of first joint, obtains cuttings totally 950 strains.
Meanwhile, the Eucalypts camaldulensis,E. seedlings that cuttage gained nursery stock is cut gained after semi-lignified spray is for the first time as new DunneEucalyptus maternal plant, continues to cultivate 60 days, and this maternal plant sprouts 2～4 side shoots, and collateral generation is to 10cm～30cm, cuts half woodenChange spray, gained semi-lignified spray is as new E. dunnii cuttings, and the 3000 strain E. dunnii cuttings that are total to newly, as new DengGrace eucalyptus cuttings is carried out cuttage.
Merge gained 950 strains and 3000 strain E. dunnii cuttings, obtain the new E. dunnii cuttings of 3950 strains, according to identical methodCarry out cuttage, cultivation, after 60 days, obtain 100% new E. dunnii cuttings, 3950 seedlings of having taken root to obtain. Cut semi-lignified sprayEucalypts camaldulensis,E. seedlings continue to cultivate 30 days-60 days as new E. dunnii maternal plant, sprout 2～4 side shoots, for providing newE. dunnii cuttings. So repeat 4 times, collect all Eucalypts camaldulensis,E. seedlingses, obtain 13000 strain E. dunnii cuttage seeding, the whole cycle needsTake 150 days.
The E. dunnii cuttage seeding of gained, after hardening, is transplanted to land for growing field crops the E. dunnii cuttage seeding of taking root for makingWoods, according to normal forest management method management E. dunnii, and records the growing state of E. dunnii growing state, overall seedling woods,In table 2.
Embodiment 10 utilizes plant growth regulator to carry out E. dunnii and grows seedlings
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.2g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 5:3, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1mg/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mmol/L.
Build steel structural plastic booth: the method for building steel structural plastic booth providing with embodiment 8 is identical.
Preparing nursery bed: the method for the preparing nursery bed providing with embodiment 8 is identical.
Prepare nutrient bag: Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, in percent by volume, rice husk ash is usedAmount is 30%, wherein the Fertility Parameters of yellow soil in Nutrition Soil: ammonium state N:30mg/L, available P: 20mg/L, effective K:200mg/L; Rice husk ash Fertility Parameters: ammonium state N:20mg/L, available P: 15mg/L, effective K:600mg/L. By Nutrition Soil fill with in 7cm ×In the Plastic Flat pocket of 11cm, then put in seedbed water and irrigate, for subsequent use.
Utilize the first plant growth regulator and the second growth regulator to process E. dunnii cuttings, prepare altogether 117000Strain E. dunnii cuttage seeding.
Cuttage and seedling culture operation:
Collect E. dunnii cuttings, it comes from Deng of the E. dunnii choiceness of cultivating at Zu Pei center, botanical garden, Hunan ProvinceGrace eucalyptus maternal plant, is semi-lignified spray, and length is 18cm, every section of 6 true leaves, a terminal bud, and lower cut is apart from first jointDistance is 2mm～5mm, gets cuttings totally 2000 strains, for cuttage. Clamp the lower end of a strain E. dunnii cuttings with tweezers, by under cuttingsPortion puts into apart from the position of end 3cm the first plant growth regulator preparing and picks, and the time picking is 2s. WillIn the nutrient bag that after picking, the E. dunnii cuttings of gained insertion water irrigates, cuttage 1 strain E. dunnii cuttings in each nutrient bag,Cutting density is 1200 strains/m2, cultivate.
In incubation, should be noted that and shade, be specially: be covered in the little arch in seedbed with the shade net that light transmittance is 5%On frame, little bow member is the bamboo cane sheet that is across the wide 2cm left and right in seedbed, its edge, insertion seedbed, two ends, and interval 70cm left and right,The arched frame of high 50cm left and right in the middle of forming.
After cultivating 8 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 8 days, waters altogether 4 times afterwards, checks DengThe situation of taking root of grace eucalyptus cuttings, finds that E. dunnii cuttings takes root, topdress afterwards, diseases prevention, mildew-resistant, insect protected management so thatEucalypts camaldulensis,E. seedlings Fast Growth.
The fertilizer used that topdresses is every liter of 3g urea, 1g potassium dihydrogen phosphate, 0.3g ferrous sulfate. After fertilizing method is executed for faling apartIrrigate with clear water.
Diseases prevention, fungus-proof measure are as follows:
A), because malnutrition causes trunk, each side shoot base portion and bottom leaf Huang, use fertilizer for spraying on leaf surface and plant nutrition liquidSupply with root and branches and leaves nutrient, reduce the nutrient consumption of tree body; B) leaf roll, spraying increases humidity, suitably shades; C) leaf blight is anti-Control, first will avoid insect pest or environment to be not suitable for caused wound, reduce artificially friction or the collision for plant; Summer is highWen Shi, suitably shades to reduce temperature; The relative air humidity of controlling well also sprays bactericidal agent for preventing and treating in time, with 25% carbendazim700 times of liquid spray, continuous spraying 3 times in 18 days, and upper and lower position sprays; D) control of black patches disease, notes strengthening branch shaping and repaiiesCut, the logical light that ventilates, when young leaves launches, sprays 800 times of liquid of carbendazim, and 10 days spray one-time continuous 4 times, remove the branch with pathogeny in timeLeaf.
Insect protected measure is as follows:
A) plant hopper: by artificial control, strengthen pruning, rationally control branch number, reduce the insect place of hiding; In conjunction with shapingPrune, cut off band worm's ovum branch and unnecessary assorted branch, prevent the overstocked concealment of branches and leaves, be beneficial to ventilation and penetrating light; Or use 10% Imidacloprid700 times of liquid spray; B) looper: the 500 times of liquid foliage-sprays of metrifonate with 15%, or spray with cypermethrin; C) mealybug: preventionMeasure, notes ventilating, and dark moist place easily grows insect pest, prunes in time overstocked branch; Medical treatment, use a red-spotted lizard mustControl.
After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtain 100% E. dunnii cuttings all take root,Growth conditions is good, and the average plant height of Eucalypts camaldulensis,E. seedlings is 23cm. Cut respectively the semi-lignified spray of these nursery stocks, gained halfLignifying spray length is 14cm, every section of 5 true leaves, a terminal bud, and lower cut is 2mm～5mm apart from the distance of first joint,Obtain cuttings totally 2000 strains, carry out cuttage as new E. dunnii cuttings. Clamp the lower end of the new E. dunnii cuttings of a strain with tweezers,The first plant growth regulator preparing is put into apart from the position of end 3cm in cuttings bottom and picks, pick timeBetween be 2s. In the nutrient bag that the E. dunnii cuttings insertion water of gained after picking is irrigated, cuttage 1 strain Deng in each nutrient bagGrace eucalyptus cuttings, cutting density is 1200 strains/m2, cultivate, in incubation, should be noted that and shade. After cultivating 8 days,Carry out moisturizing with the second plant growth regulator pouring Nutrition Soil, the humidity that keeps the Nutrition Soil in nutrient bag is 75%～95%, every second plant growth regulator of pouring in 8 days, water altogether 4 times afterwards, check the feelings of taking root of E. dunnii cuttingsCondition, finds that E. dunnii cuttings takes root, and topdresses afterwards, diseases prevention, mildew-resistant, insect protected management be so that the fast fast-growing of Eucalypts camaldulensis,E. seedlingsLong. After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 95% new E. dunnii cuttings and takes root, growsIn good condition, the average plant height of gained Eucalypts camaldulensis,E. seedlings is 23cm, cuts semi-lignified spray, and this semi-lignified spray length is15cm, every section of 4 true leaves, a terminal bud, lower cut is 2mm～5mm apart from the distance of first joint, obtains cuttings totally 1900 strains.
Meanwhile, the Eucalypts camaldulensis,E. seedlings that cuttage gained nursery stock is cut gained after semi-lignified spray is for the first time as new DunneEucalyptus maternal plant, continues to cultivate 60 days, and this maternal plant sprouts 2～4 side shoots, and collateral generation is to 10cm～30cm, cuts half woodenChange spray, gained semi-lignified spray is as new E. dunnii cuttings, and the 4100 strain E. dunnii cuttings that are total to newly, as new DengGrace eucalyptus cuttings is carried out cuttage.
Merge gained 1900 strains and 4100 strain E. dunnii cuttings, obtain the new E. dunnii cuttings of 6000 strains, according to identical sideMethod is carried out cuttage, cultivation, after 60 days, obtains 98.3% new E. dunnii cuttings, 5900 seedlings of having taken root to obtain. Cut semi-lignifiedThe Eucalypts camaldulensis,E. seedlings of spray continues to cultivate 30 days-60 days as new E. dunnii maternal plant, sprouts 2～4 side shoots, for carryingFor new E. dunnii cuttings. So repeat 6 times, collect all Eucalypts camaldulensis,E. seedlingses, obtain 117000 strain E. dunnii cuttage seeding, whole weekPhase needs 210 days.
The E. dunnii cuttage seeding of gained, after hardening, is transplanted to land for growing field crops the E. dunnii cuttage seeding of taking root for makingWoods, according to normal forest management method management E. dunnii, and records the growing state of E. dunnii growing state, overall seedling woods,In table 2.
Embodiment 11 utilizes plant growth regulator to carry out E. dunnii and grows seedlings
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.3g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 5:3, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1mg/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mmol/L.
Nutrient solution: take NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、H3BO3、ZnSO4·7H2O、Na2MoO4·2H2O、MnSO4·4H2O、Na2EDTA、FeSO4·7H2O、CuSO4·5H2O、CoCl2·6H2O, is dissolved inWater, is mixed with nutrient solution, and in gained nutrient solution, the concentration of each material is: NH4NO3180mg/L、KNO3200mg/L、KH2PO420mg/L、CaCl2·2H2O50mg/L、MgSO4·7H2O41mg/L、KI0.1mg/L、H3BO30.6mg/L、ZnSO4·7H2O0.9mg/L、Na2MoO4·2H2O0.02mg/L、MnSO40.2mg/L、Na2EDTA9mg/L、FeSO4·7H2O6mg/L、CuSO4·5H2O0.2μg/L、CoCl2·6H2O2μg/L。
Build steel structural plastic booth: the method for building steel structural plastic booth providing with embodiment 8 is identical.
Preparing nursery bed: the method for the preparing nursery bed providing with embodiment 8 is identical.
Prepare nutrient bag: Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, in percent by volume, rice husk ash is usedAmount is 40%, wherein the Fertility Parameters of yellow soil in Nutrition Soil: ammonium state N:30mg/L, available P: 20mg/L, effective K:200mg/L; Rice husk ash Fertility Parameters: ammonium state N:20mg/L, available P: 15mg/L, effective K:600mg/L. By Nutrition Soil fill with in 7cm ×In the Plastic Flat pocket of 11cm, then put in seedbed and irrigate with nutrient solution, every cubic metre of Nutrition Soil pouring 120L nutrient solution, nutritionThe humidity of soil reaches 75%-95%, for subsequent use.
Utilize the first plant growth regulator and the second growth regulator to process E. dunnii cuttings, prepare altogether 12000Strain E. dunnii cuttage seeding.
Cuttage and seedling culture operation:
Collect E. dunnii cuttings September, it comes from the good nothing of E. dunnii that cultivate at Zu Pei center, botanical garden, Hunan ProvinceThe E. dunnii maternal plant of property system, is semi-lignified spray, and length is 15cm, every section of 4 true leaves, a terminal bud, lower cut distance theThe distance of a joint is 2mm～5mm, gets cuttings totally 1000 strains, for cuttage. Clamp the lower end of a strain E. dunnii cuttings with tweezers,The first plant growth regulator preparing is put into apart from the position of end 3cm in cuttings bottom and picks, pick timeBetween be 3s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 in each nutrient bagStrain E. dunnii cuttings, cutting density is 1200 strains/m2, cultivate.
In incubation, should be noted that and shade, be specially: be covered in the little arch in seedbed with the shade net that light transmittance is 5%On frame, little bow member is the bamboo cane sheet that is across the wide 2cm left and right in seedbed, its edge, insertion seedbed, two ends, and interval 70cm left and right,The arched frame of high 50cm left and right in the middle of forming.
After cultivating 8 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 8 days, waters altogether 4 times afterwards, checks DengThe situation of taking root of grace eucalyptus cuttings, finds that E. dunnii cuttings takes root, topdress afterwards, diseases prevention, mildew-resistant, insect protected management so thatEucalypts camaldulensis,E. seedlings Fast Growth.
The fertilizer used that topdresses is potassium dihydrogen phosphate 0.3%+ carbendazim 0.1%. Fertilizing method is foliage-spray.
Diseases prevention, fungus-proof measure are as follows:
A), because malnutrition causes trunk, each side shoot base portion and bottom leaf Huang, use fertilizer for spraying on leaf surface and plant nutrition liquidSupply with root and branches and leaves nutrient, reduce the nutrient consumption of tree body; B) leaf roll, spraying increases humidity, suitably shades; C) leaf blight is anti-Control, first will avoid insect pest or environment to be not suitable for caused wound, reduce artificially friction or the collision for plant; Summer is highWen Shi, suitably shades to reduce temperature; The relative air humidity of controlling well also sprays bactericidal agent for preventing and treating in time, with 25% carbendazim700 times of liquid spray, continuous spraying 3 times in 20 days, and upper and lower position sprays; D) control of black patches disease, notes strengthening branch shaping and repaiiesCut, the logical light that ventilates, when young leaves launches, sprays 800 times of liquid of carbendazim, and 10 days spray one-time continuous 4 times, remove the branch with pathogeny in timeLeaf.
Insect protected measure is as follows:
A) plant hopper: by artificial control, strengthen pruning, rationally control branch number, reduce the insect place of hiding; In conjunction with shapingPrune, cut off band worm's ovum branch and unnecessary assorted branch, prevent the overstocked concealment of branches and leaves, be beneficial to ventilation and penetrating light; Or use 10% Imidacloprid700 times of liquid spray; B) looper: the 500 times of liquid foliage-sprays of cypermethrin with 15%, or spray with metrifonate; C) mealybug: preventionMeasure, notes ventilating, and dark moist place easily grows insect pest, prunes in time overstocked branch; Medical treatment, use a red-spotted lizard mustControl.
After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 95% E. dunnii cuttings and all takes root, gives birth toLong status is good, and the average plant height of Eucalypts camaldulensis,E. seedlings is 20cm. Cut respectively the semi-lignified spray of these nursery stocks, gained half woodMaterialization spray length is 10cm, every section of 4 true leaves, a terminal bud, and lower cut is 2mm～5mm apart from the distance of first joint,Cuttings is totally 950 strains, carries out cuttage as new E. dunnii cuttings. Clamp the lower end of the new E. dunnii cuttings of a strain with tweezers, willCuttings bottom is put into apart from the position of end 5cm the first plant growth regulator preparing and is picked, the time pickingFor 3s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 strain in each nutrient bagE. dunnii cuttings, cutting density is 1200 strains/m2, cultivate, in incubation, should be noted that and shade. Cultivate 8 days itAfter, carry out moisturizing with the second plant growth regulator pouring Nutrition Soil, the humidity that keeps the Nutrition Soil in nutrient bag is 75%～95%, every second plant growth regulator of pouring in 8 days, water altogether 4 times afterwards, check the feelings of taking root of E. dunnii cuttingsCondition, finds that E. dunnii cuttings takes root, and topdresses afterwards, diseases prevention, mildew-resistant, insect protected management be so that the fast fast-growing of Eucalypts camaldulensis,E. seedlingsLong. After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 98% new E. dunnii cuttings and takes root, growsIn good condition, the average plant height of gained Eucalypts camaldulensis,E. seedlings is 20cm, cuts semi-lignified spray, and this semi-lignified spray length is10cm, every section of 4 true leaves, a terminal bud, lower cut is 2mm～5mm apart from the distance of first joint, obtains cuttings totally 931 strains.
Meanwhile, the Eucalypts camaldulensis,E. seedlings that cuttage gained nursery stock is cut gained after semi-lignified spray is for the first time as new DunneEucalyptus maternal plant, continues to cultivate 60 days, and this maternal plant sprouts 2～4 side shoots, and collateral generation is to 10cm～30cm, cuts half woodenChange spray, gained semi-lignified spray is as new E. dunnii cuttings, and the 3900 strain E. dunnii cuttings that are total to newly, as new DengGrace eucalyptus cuttings is carried out cuttage.
Merge gained 931 strains and 3900 strain E. dunnii cuttings, obtain the new E. dunnii cuttings of 4831 strains, according to identical methodCarry out cuttage, cultivation, after 60 days, obtain 100% new E. dunnii cuttings, 4831 seedlings of having taken root to obtain. Cut semi-lignified sprayEucalypts camaldulensis,E. seedlings continue to cultivate 30 days-60 days as new E. dunnii maternal plant, sprout 2～4 side shoots, for providing newE. dunnii cuttings. So repeat 4 times, collect all Eucalypts camaldulensis,E. seedlingses, obtain 12000 strain E. dunnii cuttage seeding, the whole cycle needsTake 150 days.
The E. dunnii cuttage seeding of gained, after hardening, is transplanted to land for growing field crops the E. dunnii cuttage seeding of taking root for makingWoods, according to normal forest management method management E. dunnii, and records the growing state of E. dunnii growing state, overall seedling woods,In table 2.
Embodiment 12 utilizes plant growth regulator to carry out E. dunnii and grows seedlings
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 2mg/L, wherein indolebutyric acid andThe mass ratio of methyl α-naphthyl acetate is 5:3, the NaOH solution that solvent is 1mmol/L.
Nutrient solution: take NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、H3BO3、ZnSO4·7H2O、Na2MoO4·2H2O、MnSO4·4H2O、Na2EDTA、FeSO4·7H2O、CuSO4·5H2O、CoCl2·6H2O, is dissolved inWater, is mixed with nutrient solution, and in gained nutrient solution, the concentration of each material is: NH4NO3220mg/L、KNO3250mg/L、KH2PO424mg/L、CaCl2·2H2O60mg/L、MgSO4·7H2O50mg/L、KI0.9mg/L、H3BO30.9mg/L、ZnSO4·7H2O1.2mg/L、Na2MoO4·2H2O0.04mg/L、MnSO43.7mg/L、Na2EDTA11mg/L、FeSO4·7H2O8mg/L、CuSO4·5H2O0.4μg/L、CoCl2·6H2O4μg/L。
Build steel structural plastic booth: the method for building steel structural plastic booth providing with embodiment 8 is identical.
Preparing nursery bed: the method for the preparing nursery bed providing with embodiment 8 is identical.
Prepare nutrient bag: Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, in percent by volume, rice husk ash is usedAmount is 20%, wherein the Fertility Parameters of yellow soil in Nutrition Soil: ammonium state N:15mg/L, available P: 10mg/L, effective K:100mg/L; Rice husk ash Fertility Parameters: ammonium state N:40mg/L, available P: 25mg/L, effective K:700mg/L. By Nutrition Soil fill with in 7cm ×In the Plastic Flat pocket of 11cm, then put in seedbed and irrigate with nutrient solution, every cubic metre of Nutrition Soil pouring 120L nutrient solution, makes battalionSupport native humidity and reach 75%-95%, for subsequent use.
Utilize the first plant growth regulator and the second growth regulator to process E. dunnii cuttings, prepare altogether 28000Strain E. dunnii cuttage seeding.
Cuttage and seedling culture operation:
Collect E. dunnii cuttings April, it comes from the good nothing of E. dunnii that cultivate at Zu Pei center, botanical garden, Hunan ProvinceThe E. dunnii maternal plant of property system, is semi-lignified spray, and length is 20cm, every section of 6 true leaves, a terminal bud, lower cut distance theThe distance of a joint is 2mm～5mm, gets cuttings totally 2000 strains, for cuttage. Clamp the lower end of a strain E. dunnii cuttings with tweezers,The first plant growth regulator preparing is put into apart from the position of end 5cm in cuttings bottom and picks, pick timeBetween be 1s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 in each nutrient bagStrain E. dunnii cuttings, cutting density is 1000 strains/m2, cultivate.
In incubation, should be noted that and shade, be specially: be covered in the little arch in seedbed with the shade net that light transmittance is 5%On frame, little bow member is the bamboo cane sheet that is across the wide 1.5cm left and right in seedbed, its edge, insertion seedbed, two ends, a 65cm left side, intervalThe right side, the arched frame of high 45cm left and right in the middle of forming.
After cultivating 5 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 5 days, waters altogether 6 times afterwards, checks DengThe situation of taking root of grace eucalyptus cuttings, finds that E. dunnii cuttings takes root, topdress afterwards, diseases prevention, mildew-resistant, insect protected management so thatEucalypts camaldulensis,E. seedlings Fast Growth.
The fertilizer used that topdresses is potassium dihydrogen phosphate 0.3%+ carbendazim 0.1%. Fertilizing method is foliage-spray.
Diseases prevention, fungus-proof measure are as follows:
A), because malnutrition causes trunk, each side shoot base portion and bottom leaf Huang, use fertilizer for spraying on leaf surface and plant nutrition liquidSupply with root and branches and leaves nutrient, reduce the nutrient consumption of tree body; B) leaf roll, spraying increases humidity, suitably shades; C) leaf blight is anti-Control, first will avoid insect pest or environment to be not suitable for caused wound, reduce artificially friction or the collision for plant; Summer is highWen Shi, suitably shades to reduce temperature; The relative air humidity of controlling well also sprays bactericidal agent for preventing and treating in time, with 25% carbendazim600 times of liquid spray, continuous spraying 3 times in 20 days, and upper and lower position sprays; D) control of black patches disease, notes strengthening branch shaping and repaiiesCut, the logical light that ventilates, when young leaves launches, sprays 500 times of liquid of carbendazim, and 10 days spray one-time continuous 3 times, remove the branch with pathogeny in timeLeaf.
Insect protected measure is as follows:
A) plant hopper: by artificial control, strengthen pruning, rationally control branch number, reduce the insect place of hiding; In conjunction with shapingPrune, cut off band worm's ovum branch and unnecessary assorted branch, prevent the overstocked concealment of branches and leaves, be beneficial to ventilation and penetrating light; Or use 10% Imidacloprid500 times of liquid spray; B) looper: the 1000 times of liquid foliage-sprays of cypermethrin with 15%, or spray with metrifonate; C) mealybug: pre-Anti-measure, notes ventilating, and dark moist place easily grows insect pest, prunes in time overstocked branch; Medical treatment, uses a red-spotted lizardMust control.
After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 95% E. dunnii cuttings and all takes root, gives birth toLong status is good, and the average plant height of Eucalypts camaldulensis,E. seedlings is 30cm. Cut respectively the semi-lignified spray of these nursery stocks, gained half woodMaterialization spray length is 12cm, every section of 4 true leaves, a terminal bud, and lower cut is 2mm～5mm apart from the distance of first joint,Cuttings is totally 1900 strains, carries out cuttage as new E. dunnii cuttings. Clamp the lower end of the new E. dunnii cuttings of a strain with tweezers, willCuttings bottom is put into apart from the position of end 4cm the first plant growth regulator preparing and is picked, the time pickingFor 1s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 strain in each nutrient bagE. dunnii cuttings, cutting density is 1000 strains/m2, cultivate, in incubation, should be noted that and shade. Cultivate 5 days itAfter, carry out moisturizing with the second plant growth regulator pouring Nutrition Soil, the humidity that keeps the Nutrition Soil in nutrient bag is 75%～95%, every second plant growth regulator of pouring in 5 days, water altogether 6 times afterwards, check the feelings of taking root of E. dunnii cuttingsCondition, finds that E. dunnii cuttings takes root, and topdresses afterwards, diseases prevention, mildew-resistant, insect protected management be so that the fast fast-growing of Eucalypts camaldulensis,E. seedlingsLong. After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 95% new E. dunnii cuttings and takes root, growsIn good condition, the average plant height of gained Eucalypts camaldulensis,E. seedlings is 30cm, cuts semi-lignified spray, and this semi-lignified spray length is12cm, every section of 4 true leaves, a terminal bud, lower cut is 2mm～5mm apart from the distance of first joint, obtains cuttings totally 1805 strains.
Meanwhile, the Eucalypts camaldulensis,E. seedlings that cuttage gained nursery stock is cut gained after semi-lignified spray is for the first time as new DunneEucalyptus maternal plant, continues to cultivate 60 days, and this maternal plant sprouts 2～4 side shoots, and collateral generation is to 10cm～30cm, cuts half woodenChange spray, gained semi-lignified spray is as new E. dunnii cuttings, and the 4195 strain E. dunnii cuttings that are total to newly, as new DengGrace eucalyptus cuttings is carried out cuttage.
Merge gained 1805 strains and 4195 strain E. dunnii cuttings, obtain the new E. dunnii cuttings of 6000 strains, according to identical sideMethod is carried out cuttage, cultivation, after 60 days, obtains 95% new E. dunnii cuttings, 5700 seedlings of having taken root to obtain. Cut semi-lignified tenderThe Eucalypts camaldulensis,E. seedlings of branch continues to cultivate 30 days-60 days as new E. dunnii maternal plant, sprouts 2～4 side shoots, for providingNew E. dunnii cuttings. So repeat 4 times, collect all Eucalypts camaldulensis,E. seedlingses, obtain 28000 strain E. dunnii cuttage seeding, the whole cycleNeed 150 days.
The E. dunnii cuttage seeding of gained, after hardening, is transplanted to land for growing field crops the E. dunnii cuttage seeding of taking root for makingWoods, according to normal forest management method management E. dunnii, and records the growing state of E. dunnii growing state, overall seedling woods,In table 2.
Embodiment 13 utilizes plant growth regulator to carry out E. dunnii and grows seedlings
The first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1.6g/L, wherein indolebutyric acidWith the mass ratio of methyl α-naphthyl acetate be 5:3, the NaOH solution that solvent is 1mol/L.
The second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L, wherein indoles fourthThe mass ratio of acid and methyl α-naphthyl acetate is 3:2, the NaOH solution that solvent is 1mmol/L.
Nutrient solution: take NH4NO3、KNO3、KH2PO4、CaCl2·2H2O、MgSO4·7H2O、KI、H3BO3、ZnSO4·7H2O、Na2MoO4·2H2O、MnSO4·H2O、Na2EDTA、FeSO4·7H2O、CuSO4·5H2O、CoCl2·6H2O, water-soluble,Be mixed with nutrient solution, in gained nutrient solution, the concentration of each material is: NH4NO3200mg/L、KNO3220mg/L、KH2PO422mg/L、CaCl2·2H2O55mg/L、MgSO4·7H2O45mg/L、KI0.5mg/L、H3BO30.8mg/L、ZnSO4·7H2O1.0mg/L、Na2MoO4·2H2O0.03mg/L、MnSO4·4H2O2.0mg/L、Na2EDTA10mg/L、FeSO4·7H2O7mg/L、CuSO4·5H2O0.3μg/L、CoCl2·6H2O3μg/L。
Build steel structural plastic booth: the method for building steel structural plastic booth providing with embodiment 8 is identical.
Preparing nursery bed: the method for the preparing nursery bed providing with embodiment 8 is identical.
Prepare nutrient bag: Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, in percent by volume, rice husk ash is usedAmount is 20%, wherein the Fertility Parameters of yellow soil in Nutrition Soil: ammonium state N:35mg/L, available P: 25mg/L, effective K:250mg/L; Rice husk ash Fertility Parameters: ammonium state N:15mg/L, available P: 10mg/L, effective K:500mg/L. By Nutrition Soil fill with in 7cm ×In the Plastic Flat pocket of 11cm, then put in seedbed and irrigate with nutrient solution, every cubic metre of Nutrition Soil pouring 110L nutrient solution, makes battalionSupport native humidity and reach 75%-95%, for subsequent use.
Utilize the first plant growth regulator and the second growth regulator to process E. dunnii cuttings, prepare altogether 13800Strain E. dunnii cuttage seeding.
Cuttage and seedling culture operation:
Collect E. dunnii cuttings April, it comes from the good nothing of E. dunnii that cultivate at Zu Pei center, botanical garden, Hunan ProvinceThe E. dunnii maternal plant of property system, is semi-lignified spray, and length is 18cm, every section of 5 true leaves, a terminal bud, lower cut distance theThe distance of a joint is 2mm～5mm, gets cuttings totally 1000 strains, for cuttage. Clamp the lower end of a strain E. dunnii cuttings with tweezers,The first plant growth regulator preparing is put into apart from the position of end 5cm in cuttings bottom and picks, pick timeBetween be 2s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 in each nutrient bagStrain E. dunnii cuttings, cutting density is 1200 strains/m2, cultivate.
In incubation, should be noted that and shade, be specially: be covered in the little arch in seedbed with the shade net that light transmittance is 5%On frame, little bow member is the bamboo cane sheet that is across the wide 1.5cm left and right in seedbed, its edge, insertion seedbed, two ends, a 65cm left side, intervalThe right side, the arched frame of high 45cm left and right in the middle of forming.
After cultivating 6 days, water Nutrition Soil with the second plant growth regulator and carry out moisturizing, keep the nutrition in nutrient bagThe humidity of soil is 75%～95%, every second plant growth regulator of pouring in 6 days, waters altogether 5 times afterwards, checks DengThe situation of taking root of grace eucalyptus cuttings, finds that E. dunnii cuttings takes root, topdress afterwards, diseases prevention, mildew-resistant, insect protected management so thatEucalypts camaldulensis,E. seedlings Fast Growth.
The every liter of 3g urea of fertilizer used that topdresses, 1g potassium dihydrogen phosphate, 0.3g ferrous sulfate. Fertilizing method is blade face sprayExecute.
Diseases prevention, fungus-proof measure are as follows:
A), because malnutrition causes trunk, each side shoot base portion and bottom leaf Huang, use fertilizer for spraying on leaf surface and plant nutrition liquidSupply with root and branches and leaves nutrient, reduce the nutrient consumption of tree body; B) leaf roll, spraying increases humidity, suitably shades; C) leaf blight is anti-Control, first will avoid insect pest or environment to be not suitable for caused wound, reduce artificially friction or the collision for plant; Summer is highWen Shi, suitably shades to reduce temperature; The relative air humidity of controlling well also sprays bactericidal agent for preventing and treating in time, with 25% carbendazim600 times of liquid spray, continuous spraying 3 times in 20 days, and upper and lower position sprays; D) control of black patches disease, notes strengthening branch shaping and repaiiesCut, the logical light that ventilates, when young leaves launches, sprays 1000 times of liquid of carbendazim, and 10 days spray one-time continuous 3 times are removed with pathogeny in timeBranches and leaves.
Insect protected measure is as follows:
A) plant hopper: by artificial control, strengthen pruning, rationally control branch number, reduce the insect place of hiding; In conjunction with shapingPrune, cut off band worm's ovum branch and unnecessary assorted branch, prevent the overstocked concealment of branches and leaves, be beneficial to ventilation and penetrating light; Or use 10% Imidacloprid1000 times of liquid spray; B) looper: the 1000 times of liquid foliage-sprays of metrifonate with 15%, or spray with cypermethrin; C) mealybug: pre-Anti-measure, notes ventilating, and dark moist place easily grows insect pest, prunes in time overstocked branch; Medical treatment, uses a red-spotted lizardMust control.
After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 95% E. dunnii cuttings and all takes root, gives birth toLong status is good, and the average plant height of Eucalypts camaldulensis,E. seedlings is 28cm. Cut respectively the semi-lignified spray of these nursery stocks, gained half woodMaterialization spray length is 15cm, every section of 4 true leaves, a terminal bud, and lower cut is 2mm～5mm apart from the distance of first joint,Cuttings is totally 950 strains, carries out cuttage as new E. dunnii cuttings. Clamp the lower end of the new E. dunnii cuttings of a strain with tweezers, willCuttings bottom is put into apart from the position of end 5cm the first plant growth regulator preparing and is picked, the time pickingFor 2s. The E. dunnii cuttings of gained after picking is inserted in the nutrient bag irrigating with nutrient solution to cuttage 1 strain in each nutrient bagE. dunnii cuttings, cutting density is 1200 strains/m2, cultivate, in incubation, should be noted that and shade. Cultivate 6 days itAfter, carry out moisturizing with the second plant growth regulator pouring Nutrition Soil, the humidity that keeps the Nutrition Soil in nutrient bag is 75%～95%, every second plant growth regulator of pouring in 6 days, water altogether 5 times afterwards, check the feelings of taking root of E. dunnii cuttingsCondition, finds that E. dunnii cuttings takes root, and topdresses afterwards, diseases prevention, mildew-resistant, insect protected management be so that the fast fast-growing of Eucalypts camaldulensis,E. seedlingsLong. After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 100% new E. dunnii cuttings and takes root, growsIn good condition, the average plant height of gained Eucalypts camaldulensis,E. seedlings is 28cm, cuts semi-lignified spray, and this semi-lignified spray length is15cm, every section of 4 true leaves, a terminal bud, lower cut is 2mm～5mm apart from the distance of first joint, obtains cuttings totally 950 strains.
Meanwhile, the Eucalypts camaldulensis,E. seedlings that cuttage gained nursery stock is cut gained after semi-lignified spray is for the first time as new DunneEucalyptus maternal plant, continues to cultivate 60 days, and this maternal plant sprouts 2～4 side shoots, and collateral generation is to 10cm～30cm, cuts half woodenChange spray, gained semi-lignified spray is as new E. dunnii cuttings, and the 2000 strain E. dunnii cuttings that are total to newly, as new DengGrace eucalyptus cuttings is carried out cuttage.
Merge gained 950 strains and 2000 strain E. dunnii cuttings, obtain the new E. dunnii cuttings of 2950 strains, according to identical methodCarry out cuttage, cultivation, after 60 days, obtain 98% new E. dunnii cuttings, 2891 seedlings of having taken root to obtain. Cut semi-lignified sprayEucalypts camaldulensis,E. seedlings continue to cultivate 30 days-60 days as new E. dunnii maternal plant, sprout 2～4 side shoots, for providing newE. dunnii cuttings. So repeat 4 times, collect all Eucalypts camaldulensis,E. seedlingses, obtain 13800 strain E. dunnii cuttage seeding, the whole cycle needsTake 150 days.
The E. dunnii cuttage seeding of gained, after hardening, is transplanted to land for growing field crops the E. dunnii cuttage seeding of taking root for makingWoods, according to normal forest management method management E. dunnii, and records the growing state of E. dunnii growing state, overall seedling woods,In table 2.
Embodiment 14 adopts the quality evaluation of the Eucalypts camaldulensis,E. seedlings of E. dunnii method for culturing seedlings acquisition provided by the invention
After investigation transplanting, the growing height of the ratio of the survival rate of E. dunnii, the E. dunnii of throwing oneself on the ground, Eucalypts camaldulensis,E. seedlings is averageValue, the quality of the E. dunnii cuttage seeding preparing with Evaluation operation example 8 to embodiment 13, carries out Dunne with the following method to adoptEucalyptus cuttage and seedling culture as a control group.
Control group cuttage and seedling culture method: plant growth regulator is by ABT2 root-inducing powder+talcum powder+carbendazim, and concentration is10mg/L。
Build steel structural plastic booth: the method for building steel structural plastic booth providing with embodiment 8 is identical.
Preparing nursery bed: the method for the preparing nursery bed providing with embodiment 8 is identical.
Prepare nutrient bag: Nutrition Soil is the homogeneous mixture of yellow soil and rice husk ash, in percent by volume, rice husk ash is usedAmount is 30%, wherein the Fertility Parameters of yellow soil in Nutrition Soil: ammonium state N:30mg/L, available P: 20mg/L, effective K:200mg/L; Rice husk ash Fertility Parameters: ammonium state N:20mg/L, available P: 15mg/L, effective K:600mg/L. By Nutrition Soil fill with in 7cm ×In the Plastic Flat pocket of 11cm, then put in seedbed water and irrigate, for subsequent use.
Utilize plant growth regulator treatment E. dunnii cuttings, preparation obtains 6000 strain E. dunnii cuttage seeding altogether.
Cuttage and seedling culture operation:
Collect E. dunnii cuttings, it comes from Deng of the E. dunnii choiceness of cultivating at Zu Pei center, botanical garden, Hunan ProvinceGrace eucalyptus maternal plant, is semi-lignified spray, and length is 18cm, every section of 5 true leaves, a terminal bud, and lower cut is apart from first jointDistance is 2mm～5mm, gets cuttings totally 1000 strains, for cuttage. Cuttings bottom is put into and prepared apart from the position of end 3cmIn plant growth regulator, soak, the time of immersion is 30m. . The E. dunnii cuttings of gained after soaking is inserted to waterIn the nutrient bag irrigating, cuttage 1 strain E. dunnii cuttings in each nutrient bag, cutting density is 1200 strains/m2, cultivate.
In incubation, should be noted that and shade, be specially: be covered in the little arch in seedbed with the shade net that light transmittance is 5%On frame, little bow member is the bamboo cane sheet that is across the wide 2cm left and right in seedbed, its edge, insertion seedbed, two ends, and interval 70cm left and right,The arched frame of high 50cm left and right in the middle of forming.
After cultivating 8 days, water pouring Nutrition Soil carries out moisturizing, and the humidity that keeps the Nutrition Soil in nutrient bag is 75%～95%, every a water of pouring in 8 days, water altogether 4 times afterwards, check the situation of taking root of E. dunnii cuttings, find that E. dunnii is slottingFringe is taken root, and topdresses afterwards, diseases prevention, mildew-resistant, insect protected management be so that Eucalypts camaldulensis,E. seedlings Fast Growth.
The fertilizer used that topdresses is every liter of 3g urea, 1g potassium dihydrogen phosphate, 0.3g ferrous sulfate. After fertilizing method is executed for faling apartIrrigate with clear water.
Diseases prevention, fungus-proof measure are as follows:
A), because malnutrition causes trunk, each side shoot base portion and bottom leaf Huang, use fertilizer for spraying on leaf surface and plant nutrition liquidSupply with root and branches and leaves nutrient, reduce the nutrient consumption of tree body; B) leaf roll, spraying increases humidity, suitably shades; C) leaf blight is anti-Control, first will avoid insect pest or environment to be not suitable for caused wound, reduce artificially friction or the collision for plant; Summer is highWen Shi, suitably shades to reduce temperature; The relative air humidity of controlling well also sprays bactericidal agent for preventing and treating in time, with 25% carbendazim700 times of liquid spray, continuous spraying 3 times in 18 days, and upper and lower position sprays; D) control of black patches disease, notes strengthening branch shaping and repaiiesCut, the logical light that ventilates, when young leaves launches, sprays 800 times of liquid of carbendazim, and 10 days spray one-time continuous 4 times, remove the branch with pathogeny in timeLeaf.
Insect protected measure is as follows:
A) plant hopper: by artificial control, strengthen pruning, rationally control branch number, reduce the insect place of hiding; In conjunction with shapingPrune, cut off band worm's ovum branch and unnecessary assorted branch, prevent the overstocked concealment of branches and leaves, be beneficial to ventilation and penetrating light; Or use 10% Imidacloprid700 times of liquid spray; B) looper: the 500 times of liquid foliage-sprays of metrifonate with 15%, or spray with cypermethrin; C) mealybug: preventionMeasure, notes ventilating, and dark moist place easily grows insect pest, prunes in time overstocked branch; Medical treatment, use a red-spotted lizard mustControl.
After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 40% E. dunnii cuttings and all takes root, growsIn good condition, the average plant height of Eucalypts camaldulensis,E. seedlings is 23cm. The semi-lignified spray of cutting respectively these nursery stocks, gained half is woodenChanging spray length is 14cm, every section of 5 true leaves, a terminal bud, and lower cut is 2mm～5mm apart from the distance of first joint, obtains slottingFringe is totally 400 strains, carries out cuttage as new E. dunnii cuttings. Cuttings bottom is put into and prepared apart from the position of end 5cmIn plant growth regulator, soak, the time of immersion is 30m. The E. dunnii cuttings of gained after soaking is inserted to water watersIn saturating nutrient bag, cuttage 1 strain E. dunnii cuttings in each nutrient bag, cutting density is 1200 strains/m2, cultivate, in trainingIn the process of supporting, should be noted that and shade. After cultivating 8 days, water pouring Nutrition Soil carries out moisturizing, keeps the Nutrition Soil in nutrient bagHumidity be 75%～95%, every a water of pouring in 8 days, water altogether 4 times afterwards, check the feelings of taking root of E. dunnii cuttingsCondition, finds that E. dunnii cuttings takes root, and topdresses afterwards, diseases prevention, mildew-resistant, insect protected management be so that the fast fast-growing of Eucalypts camaldulensis,E. seedlingsLong. After the cultivation of 60 days, statistics Eucalypts camaldulensis,E. seedlings growing state, obtains 40% new E. dunnii cuttings and takes root, growsIn good condition, the average plant height of gained Eucalypts camaldulensis,E. seedlings is 23cm, cuts semi-lignified spray, and this semi-lignified spray length is14cm, every section of 4 true leaves, a terminal bud, lower cut is 2mm～5mm apart from the distance of first joint, obtains cuttings totally 160 strains, doesCarry out cuttage for new E. dunnii cuttings, the Eucalypts camaldulensis,E. seedlings of cutting gained after semi-lignified spray continues to cultivate.
Meanwhile, the Eucalypts camaldulensis,E. seedlings that cuttage gained nursery stock is cut gained after semi-lignified spray is for the first time as new DunneEucalyptus maternal plant, continues to cultivate 60 days, and this maternal plant sprouts 2～4 side shoots, and collateral generation is to 10cm～30cm, cuts half woodenChange spray, gained semi-lignified spray is as new E. dunnii cuttings, and the 1540 strain E. dunnii cuttings that are total to newly, as new DengGrace eucalyptus cuttings is carried out cuttage.
Merge gained 160 strains and 1540 strain E. dunnii cuttings, obtain the new E. dunnii cuttings of 1700 strains, according to identical methodCarry out cuttage, cultivation, after 60 days, obtain 45% new E. dunnii cuttings, 765 seedlings of having taken root to obtain. Cut semi-lignified sprayEucalypts camaldulensis,E. seedlings continues to cultivate 30 days-60 days as new E. dunnii maternal plant, sprouts 2～4 side shoots, for providing newE. dunnii cuttings. So repeat 4 times, collect all Eucalypts camaldulensis,E. seedlingses, obtain 6000 strain E. dunnii cuttage seeding, the whole cycle needs150 days.
The E. dunnii cuttage seeding of gained, after hardening, is transplanted to land for growing field crops the E. dunnii cuttage seeding of taking root for makingWoods, according to normal forest management method management E. dunnii, and records the growing state of E. dunnii growing state, overall seedling woods,In table 2.
When the E. dunnii cuttage seeding that embodiment 8 to embodiment 13, control group obtain is used for afforesting, each group is cultivated and is obtainedE. dunnii cuttage seeding for the evaluation result of planting trees in table 2.
Each group of table 2 is cultivated the evaluation result of the Eucalypts camaldulensis,E. seedlings obtaining for planting trees
From table 2, experimental data is known, compares control group, the Eucalypts camaldulensis,E. seedlings that embodiment 8 to embodiment 13 preparesWhen planting trees, after transplanting, survival rate significantly improves, significant difference (P < 0.05); The phenomenon of throwing oneself on the ground be improved significantly,The ratio of E. dunnii of throwing oneself on the ground obviously reduces, significant difference (P < 0.05); Transplant that the growth of rear E. dunnii is fast, the bodily form is graceful, instituteThe E. dunnii forest form obtaining is neat, is more conducive to the growth of E. dunnii.
Below be only the preferred embodiment of the present invention, it should be pointed out that above-mentioned preferred embodiment should not be considered as rightRestriction of the present invention, protection scope of the present invention should be as the criterion with claim limited range. For the artThose of ordinary skill, without departing from the spirit and scope of the present invention, can also make some improvements and modifications, and these changeEnter and retouch and also should be considered as protection scope of the present invention.
Claims (5)
1. for a plant growth regulator for E. dunnii cuttage and seedling culture, it is characterized in that, it comprises the first plant growth tuneJoint agent and the second plant growth regulator;
Described the first plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 1g/L～1.6g/L;
Described the second plant growth regulator is made up of indolebutyric acid and methyl α-naphthyl acetate, and its concentration is 0.83mg/L～2mg/L;
In described the first plant growth regulator, the mass ratio of indolebutyric acid and methyl α-naphthyl acetate is 3:2 or 5:3;
In described the second plant growth regulator, the mass ratio of indolebutyric acid and methyl α-naphthyl acetate is 3:2 or 5:3.
2. plant growth regulator according to claim 1, is characterized in that, it also comprises nutrient solution, described nutrient solutionComprise following component:
3. utilize plant growth regulator as claimed in claim 1 to carry out the method for E. dunnii cuttage and seedling culture, its featureBe, comprise the following steps:
Step 1: obtain E. dunnii cuttings;
Step 2: get described E. dunnii cuttings and pick after described the first plant growth regulator, cuttage is in Nutrition Soil;
Step 3: water described the second plant growth regulator, cultivate, obtain E. dunnii cuttage seeding;
The frequency of described pouring be every 5～8 days once, the number of times of described pouring is 4 times～6 times.
4. method according to claim 3, is characterized in that, the time picking described in step 2 is 1 second～3 seconds.
5. method according to claim 3, is characterized in that, the amount of pouring described in step 3 is wet with described Nutrition SoilDegree reaches 75%～95% and is as the criterion.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067549A (en) * | 1992-05-08 | 1993-01-06 | 深圳市南山区园林绿化公司 | A kind of fast asexual propagation method of eucalyptus |
| CN1381171A (en) * | 2002-05-22 | 2002-11-27 | 湖南省森林植物园 | Tissue culture method for breeding Dengen eucalyptus |
-
2014
- 2014-07-09 CN CN201410324723.7A patent/CN104082299B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067549A (en) * | 1992-05-08 | 1993-01-06 | 深圳市南山区园林绿化公司 | A kind of fast asexual propagation method of eucalyptus |
| CN1381171A (en) * | 2002-05-22 | 2002-11-27 | 湖南省森林植物园 | Tissue culture method for breeding Dengen eucalyptus |
Non-Patent Citations (2)
| Title |
|---|
| "邓恩桉种子育苗扦插扩繁技术";林成立;《福建林业科技》;20050220;第32卷(第2期);第80-84页 * |
| "邓恩桉种子育苗扦插扩繁技术";汪昌国;《湖南林业科技》;20040220;第31卷(第2期);第42-45页 * |
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