CN104041530B - A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof - Google Patents

A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof Download PDF

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CN104041530B
CN104041530B CN201410235002.9A CN201410235002A CN104041530B CN 104041530 B CN104041530 B CN 104041530B CN 201410235002 A CN201410235002 A CN 201410235002A CN 104041530 B CN104041530 B CN 104041530B
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bactericidal agent
strawberry leaves
natural bactericidal
rot
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CN104041530A (en
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赵兰
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Abstract

A kind of natural bactericidal agent preventing and treating strawberry leaves rot provided by the invention, is made up of following component: camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant, the banksia rose.The natural bactericidal agent abundant raw material source of this control strawberry leaves rot, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat strawberry leaves rot.

Description

A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof.
Background technology
Strawberry leaves rot is sick also known as purple spot, focal spot, and China occurs commonplace, and be one of strawberry leaves portion Common Diseases, occurrence injury is quite serious sometimes.Strawberry leaves rot cause of disease is pineapple strawberry brown patch germ Marssoninapotentillae (Desmazieres) Magn, and the perfect stage is that Diplocarponearliana (ElletEV.) belongs to Ascomycotina.Germ survives the winter in plant diseased tissues or land on invalid thing with perithecium or pycnidium, discharges ascospore spring or conidium borrows air to carry out diffusive transport, infect morbidity, and by during seedling carries out in spite of illness, long-distance communications.This disease belongs to low temperature properties disease, and autumn and the more weather of rain and dew in early spring are conducive to infecting morbidity.The strong morbidity of fertile sufficient seedling is light, and the weak morbidity of fertilizer deficiency seedling is heavy.Between strawberry cultivars, disease resistance is variant, and new star, to reach the kinds such as Na more disease-resistant.
Strawberry leaves rot mainly in morbidity in season in spring and autumn, encroaches on blade, petiole, carpopodium and calyx.Blade produces the matt fleck of puce, be expanded into the irregular shape scab of diameter 3-4 millimeters later, scab central authorities change not quite with periphery color, scab has the tendency along vein distribution, when morbidity is heavy, blade face is covered with scab, and the later stage, full leaf was yellowish-brown to crineous, until withered.Grow black small grain point in the withered part of scab, after petiole or carpopodium are fallen ill, produce the scab that pitchy slightly caves in, sick portion tissue becomes fragile and frangibility.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for control strawberry leaves rot is normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control strawberry leaves rot that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating strawberry leaves rot provided by the invention, is made up of following component: camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant, the banksia rose.
Be made up of the component of following weight portion: camomile 10-20 part, frutus cnidii 8-12 part, lotus leaf 8-12 part, yellow pine 2-4 part, Chinese honey locust 2-4 part, tarragon 2-4 part, Celastrus angulatus 2-4 part, Pogostemon cablin 1-3 part, stem pith of the rice-paper plant 1-3 part, banksia rose 1-3 part.
Be made up of the component of following weight portion: camomile 15 parts, frutus cnidii 10 parts, 10 parts, lotus leaf, yellow pine 3 parts, Chinese honey locust 3 parts, tarragon 3 parts, Celastrus angulatus 3 parts, Pogostemon cablin 2 parts, the stem pith of the rice-paper plant 2 parts, the banksia rose 2 parts.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control strawberry leaves rot, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
Present invention also offers the application of the natural bactericidal agent of above-mentioned control strawberry leaves rot, be specially: the natural bactericidal agent of control strawberry leaves rot is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control strawberry leaves rot provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat strawberry leaves rot.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control strawberry leaves rot, is made up of the component of following weight portion: camomile 15 parts, frutus cnidii 10 parts, 10 parts, lotus leaf, yellow pine 3 parts, Chinese honey locust 3 parts, tarragon 3 parts, Celastrus angulatus 3 parts, Pogostemon cablin 2 parts, the stem pith of the rice-paper plant 2 parts, the banksia rose 2 parts
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
Embodiment 2
The natural bactericidal agent of control strawberry leaves rot, is made up of the component of following weight portion: camomile 20 parts, frutus cnidii 8 parts, 8 parts, lotus leaf, yellow pine 2 parts, Chinese honey locust 4 parts, tarragon 4 parts, Celastrus angulatus 4 parts, Pogostemon cablin 3 parts, the stem pith of the rice-paper plant 3 parts, the banksia rose 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
Embodiment 3
The natural bactericidal agent of control strawberry leaves rot, is made up of the component of following weight portion: camomile 10 parts, frutus cnidii 12 parts, 12 parts, lotus leaf, yellow pine 4 parts, Chinese honey locust 2 parts, tarragon 2 parts, Celastrus angulatus 2 parts, Pogostemon cablin 1 part, the stem pith of the rice-paper plant 1 part, the banksia rose 3 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
Embodiment 4 measures the natural bactericidal agent of the control strawberry leaves rot of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent of control strawberry leaves rot of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Marssoninapotentillae (Desmazieres) Magn is commercially available for pineapple strawberry brown patch germ.
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of pineapple strawberry brown patch germ Marssoninapotentillae (Desmazieres) Magn, by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment strawberry leaves rot of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry leaves rot of table 3 embodiment 1 to 3

Claims (4)

1. prevent and treat a natural bactericidal agent for strawberry leaves rot, it is characterized in that: be made up of the component of following weight portion: camomile 5-15 part, frutus cnidii 5-15 part, lotus leaf 8-12 part, yellow pine 8-12 part, Chinese honey locust 2-4 part, tarragon 2-4 part, Celastrus angulatus 2-4 part, Pogostemon cablin 1-3 part, stem pith of the rice-paper plant 1-3 part, banksia rose 1-3 part;
Wherein, the preparation method of the natural bactericidal agent of described control strawberry leaves rot, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
2. a kind of natural bactericidal agent preventing and treating strawberry leaves rot according to claim 1, is characterized in that: be made up of the component of following weight portion: camomile 10 parts, frutus cnidii 10 parts, 10 parts, lotus leaf, yellow pine 10 parts, Chinese honey locust 3 parts, tarragon 3 parts, Celastrus angulatus 3 parts, Pogostemon cablin 2 parts, the stem pith of the rice-paper plant 2 parts, the banksia rose 2 parts.
3. a preparation method for the natural bactericidal agent of the control strawberry leaves rot described in claim 1 or 2, is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, frutus cnidii, lotus leaf, yellow pine, Chinese honey locust, tarragon, Celastrus angulatus, Pogostemon cablin, the stem pith of the rice-paper plant and the banksia rose;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry leaves rot.
4. the application of natural bactericidal agent in control strawberry leaves rot of the control strawberry leaves rot described in claim 1 or 2, is characterized in that: mixed with 500-1000 times of water gaging by the natural bactericidal agent of control strawberry leaves rot and be sterilization microbial inoculum.
CN201410235002.9A 2014-05-29 2014-05-29 A kind ofly prevent and treat natural bactericidal agent of strawberry leaves rot and preparation method thereof Active CN104041530B (en)

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CN105028530A (en) * 2015-06-02 2015-11-11 张亚南 Botanical pesticide for strawberry production
CN105010423A (en) * 2015-06-26 2015-11-04 安徽禾众农业科技有限公司 Strawberry leaf blight prevention and treatment method
CN105053082A (en) * 2015-07-15 2015-11-18 刘晓 Drug for preventing and treating michelia figo leaf blight
CN106070206A (en) * 2016-06-14 2016-11-09 重庆鸿钜农业科技发展有限公司 A kind of compositions preventing and treating lawn pinta in summer
CN105994451A (en) * 2016-07-29 2016-10-12 广西素安生态农业有限公司 Chinese herbal medicine control agent for common diseases of strawberries and preparation method thereof
CN106577719A (en) * 2016-11-24 2017-04-26 广西大学 Bactericide composite for preventing and controlling gingko leaf blight

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CN1452869A (en) * 2002-04-26 2003-11-05 郭立卿 Prepn of wide-spectrum biopesticide of Chinese herbal medicine
KR20090047563A (en) * 2007-10-30 2009-05-13 전라남도 Botanical pesticide from chrysanthemum spp. extract with insecticidal soap to control of strawberry pest
CN101869122A (en) * 2010-06-01 2010-10-27 刘志慧 Garlicin plant growth promoting solution with insect disinfestation and sterilization functions

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