CN104012581B - A kind ofly prevent and treat natural bactericidal agent of strawberry zonate spot and preparation method thereof - Google Patents
A kind ofly prevent and treat natural bactericidal agent of strawberry zonate spot and preparation method thereof Download PDFInfo
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- CN104012581B CN104012581B CN201410238461.2A CN201410238461A CN104012581B CN 104012581 B CN104012581 B CN 104012581B CN 201410238461 A CN201410238461 A CN 201410238461A CN 104012581 B CN104012581 B CN 104012581B
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Abstract
A kind of natural bactericidal agent preventing and treating strawberry zonate spot provided by the invention, is made up of following component: camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle, cordate houttuynia.The natural bactericidal agent abundant raw material source of this control strawberry zonate spot, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat strawberry zonate spot.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of strawberry zonate spot and preparation method thereof.
Background technology
The harm of strawberry zonate spot is extensive, and each strawberry place of production of China generally occurs, and the morbidity of indivedual areas is serious, endangers heavier with strawberry nursery and outdoor cropping.Belong to caused by Phomopsisobscurans infects by the plan point of fungi Deuteromycotina Sphaeropsidales.Germ survives the winter in sick leaf texture or invalid body with pycnidium and mycelium in soil, becomes primary source of infection in next year.The germ that survives the winter produces a large amount of conidium when month temperature in next year 6 ~ 7 is suitable for, and infect by means of rainwater sputtering and air borne, then sick portion constantly produces conidium and repeatedly infects again, increases the weight of to endanger.
Strawberry zonate spot main harm blade, petiole and stolon.Their early stage, blade face produces aubergine fleck, and is expanded into circle or the nearly oval large scab of atropurpureus gradually, and this is this sick obvious characteristic.Scab center dark brown, around yellowish-brown, edge redness, yellow or aubergine, scab has wheel line sometimes, and the later stage there will be little black patches (i.e. germ pycnidium), and time serious, scab joins together, and causes blade withered.Scab is when blade tip, vein occur, and Chang Shi leaf texture is withered in " V " font, also known as strawberry " V " type brown spot.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for control strawberry zonate spot is normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control strawberry zonate spot that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating strawberry zonate spot provided by the invention, is made up of following component: camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle, cordate houttuynia.
Preferably, be made up of the component of following weight portion: camomile 5-15 part, Chinese prickly ash 5-15 part, lotus leaf 8-12 part, Asiatic plantain 8-12 part, peppermint 2-4 part, tarragon 2-4 part, folium isatidis 2-4 part, bur beggar-ticks 1-3 part, honeysuckle 1-3 part, cordate houttuynia 1-3 part.
More preferably, be made up of the component of following weight portion: camomile 10 parts, 10 parts, Chinese prickly ash, 10 parts, lotus leaf, Asiatic plantain 10 parts, peppermint 3 parts, tarragon 3 parts, folium isatidis 3 parts, bur beggar-ticks 2 parts, honeysuckle 2 parts, cordate houttuynia 2 parts.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control strawberry zonate spot, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle and cordate houttuynia;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry zonate spot.
Present invention also offers the application of the natural bactericidal agent of above-mentioned control strawberry zonate spot, be specially: the natural bactericidal agent of control strawberry zonate spot is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control strawberry zonate spot provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat strawberry zonate spot.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control strawberry zonate spot, is made up of the component of following weight portion: camomile 10 parts, 10 parts, Chinese prickly ash, 10 parts, lotus leaf, Asiatic plantain 10 parts, peppermint 3 parts, tarragon 3 parts, folium isatidis 3 parts, bur beggar-ticks 2 parts, honeysuckle 2 parts, cordate houttuynia 2 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle and cordate houttuynia;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry zonate spot.
Embodiment 2
The natural bactericidal agent of control strawberry zonate spot, is made up of the component of following weight portion: camomile 5 parts, 15 parts, Chinese prickly ash, 8 parts, lotus leaf, Asiatic plantain 8 parts, peppermint 4 parts, tarragon 4 parts, folium isatidis 4 parts, bur beggar-ticks 3 parts, honeysuckle 3 parts, cordate houttuynia 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle and cordate houttuynia;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry zonate spot.
Embodiment 3
The natural bactericidal agent of control strawberry zonate spot, is made up of the component of following weight portion: camomile 15 parts, 5 parts, Chinese prickly ash, 12 parts, lotus leaf, Asiatic plantain 12 parts, peppermint 2 parts, tarragon 2 parts, folium isatidis 2 parts, bur beggar-ticks 1 part, honeysuckle 1 part, cordate houttuynia 3 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle and cordate houttuynia;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry zonate spot.
Embodiment 4 measures the natural bactericidal agent of the control strawberry zonate spot of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent of control strawberry zonate spot of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Strawberry zonate spot bacterium Phomopsis (Dendrophoma) Obscurans (ElletEV.) is commercially available.
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of strawberry zonate spot bacterium Phomopsis (Dendrophoma) Obscurans (ElletEV.), by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment strawberry zonate spot of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry zonate spot of table 3 embodiment 1 to 3
Claims (3)
1. prevent and treat a natural bactericidal agent for strawberry zonate spot, it is characterized in that: be made up of the component of following weight portion: camomile 5-15 part, Chinese prickly ash 5-15 part, lotus leaf 8-12 part, Asiatic plantain 8-12 part, peppermint 2-4 part, tarragon 2-4 part, folium isatidis 2-4 part, bur beggar-ticks 1-3 part, honeysuckle 1-3 part, cordate houttuynia 1-3 part; Wherein, the preparation method of the natural bactericidal agent of described control strawberry zonate spot, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle and cordate houttuynia;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry zonate spot.
2. a kind of natural bactericidal agent preventing and treating strawberry zonate spot according to claim 1, is characterized in that: be made up of the component of following weight portion: camomile 10 parts, 10 parts, Chinese prickly ash, 10 parts, lotus leaf, Asiatic plantain 10 parts, peppermint 3 parts, tarragon 3 parts, folium isatidis 3 parts, bur beggar-ticks 2 parts, honeysuckle 2 parts, cordate houttuynia 2 parts.
3. a preparation method for the natural bactericidal agent of control strawberry zonate spot according to claim 1, is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is camomile, Chinese prickly ash, lotus leaf, Asiatic plantain, peppermint, tarragon, folium isatidis, bur beggar-ticks, honeysuckle and cordate houttuynia;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of strawberry zonate spot.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104782686A (en) * | 2015-03-05 | 2015-07-22 | 山东省花生研究所 | Peanut Chinese medicinal bactericide |
| CN104996501A (en) * | 2015-06-26 | 2015-10-28 | 安徽禾众农业科技有限公司 | Bacteriostatic agent for preventing and treating strawberry zonate spot and its preparation method and use |
| CN104982472A (en) * | 2015-06-26 | 2015-10-21 | 安徽禾众农业科技有限公司 | Prevention method for strawberry zonate spots |
| CN105519599A (en) * | 2015-12-24 | 2016-04-27 | 青岛百瑞吉生物工程有限公司 | Natural bactericide for preventing and treating angular leaf spot of persimmon trees, and preparation method thereof |
| CN105850476A (en) * | 2016-04-28 | 2016-08-17 | 郎溪县建平镇荣香瓜果种植家庭农场 | Method for preventing and treating conventional diseases of strawberries |
| CN106234457A (en) * | 2016-08-10 | 2016-12-21 | 吴远宁 | A kind of plantain tree antibacterial and preparation method thereof |
| CN106212108A (en) * | 2016-08-31 | 2016-12-14 | 北海市农业科学研究所 | The stand cultivation method of hami melon |
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| CN102696694A (en) * | 2012-05-11 | 2012-10-03 | 浙江农林大学 | Special natural bactericidal protectant for garden plants as well as use and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102696694A (en) * | 2012-05-11 | 2012-10-03 | 浙江农林大学 | Special natural bactericidal protectant for garden plants as well as use and preparation method thereof |
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| 草莓褐色轮斑病病原生物学研究及防治药剂的筛选;贾晓辉等;《安徽农业科学》;20051231;第33卷(第10期);第1849-1850页 * |
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