CN103992940A - Instrument for extracting bioactive substance - Google Patents
Instrument for extracting bioactive substance Download PDFInfo
- Publication number
- CN103992940A CN103992940A CN201410228543.9A CN201410228543A CN103992940A CN 103992940 A CN103992940 A CN 103992940A CN 201410228543 A CN201410228543 A CN 201410228543A CN 103992940 A CN103992940 A CN 103992940A
- Authority
- CN
- China
- Prior art keywords
- mounting plate
- instrument
- fixed
- active substance
- drive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides an instrument for extracting a bioactive substance. The instrument comprises a rack, a workbench, a liquid transfer assembly and a separation assembly, wherein the liquid transfer assembly comprises a first mounting plate, a first driving unit, pipettes, piston rods and a first driving component; the first driving unit is used for driving the first mounting plate to move along the height direction of the rack; the pipettes are connected on the first mounting plate; the piston rods are inserted in the pipettes and are hermetically fit with the inner walls of the pipettes; the first driving component is used for driving the piston rods to move up and down relative to the pipettes; the separation assembly comprises a second mounting plate, a second driving unit, casings, magnetic bars and a second driving component; the second driving unit is used for driving the second mounting plate to move along the height direction of the rack; the casings are fixed on the second mounting plate; the second driving component is used for driving the magnetic bars to move up and down relative to the casings; the upper ends of the casings are provided with openings, and the lower ends are blind ends; the magnetic bars are inserted in the casings from openings at the upper ends of the casings by way of clearance fit. The instrument improves the bioactive substance extraction efficiency, and the extracted sample has higher purity.
Description
Technical field
The present invention relates to a kind of instrument that extracts biologically active substance.
Background technology
In fields such as disease control, food safety, forensic identification, genomics, conventionally need to extract biological active substance (as DNA) as the object that detects, studies; The extracting method of traditional biologically active substance, normally adopt boiling method, negative pressure method etc., the extraction element structure that these traditional methods adopt is comparatively complicated, the efficiency of work is relatively low, be difficult to meet the requirement that in medical industry, batch samples is processed, and in the sample after purifying, contain not removed material in a large number, the accuracy of the structure that impact detects and studies.
Along with the appearance of novel agent, industry starts progressively to adopt paramagnetic particle method to extract at present, still, adopts at present the instrument of paramagnetic particle method extraction biologically active substance, its complex structure, and the purity after sample preparation is relatively low.
Summary of the invention
For the deficiencies in the prior art, object of the present invention is intended to provide a kind of instrument that extracts biologically active substance, and it can improve the efficiency that biologically active substance extracts, and improves the purity of processing rear sample.
For achieving the above object, the present invention adopts following technical scheme:
An instrument that extracts biologically active substance, comprises,
Frame;
Be arranged on the worktable of frame bottom, on this worktable, be provided with for placing dress sample tube, dress magnetic bead liquid test tube and dressing up pipe support and the driving mechanism for driving pipe support to move along the width of frame of product test tube;
What be positioned at worktable top moves liquid assembly, this move liquid assembly comprise the first mounting plate, for the first driver element of driving the first mounting plate to move along the short transverse of frame, be connected to transfer pipet on the first mounting plate, be inserted in transfer pipet and with the airtight piston rod coordinating of inwall and first driven unit for driving piston rod to move up and down with respect to transfer pipet of transfer pipet;
Be arranged in above worktable and for the isolated separating assembly of the sample from cracking by biologically active substance, this separating assembly comprises the second mounting plate, for the second driver element of driving the second mounting plate to move along the short transverse of frame, be fixed on sleeve pipe, magnetic bar and the second driven unit for driving magnetic bar to move up and down with respect to sleeve pipe on the second mounting plate, the upper end opening of sleeve pipe, bottom are cecum, and in the mode of running fit, the upper end opening by sleeve pipe inserts in sleeve pipe magnetic bar.
Separating assembly also comprises the second portable plate, and magnetic bar is fixed on the second portable plate, is fixed with the second guide rail extending along bracket height direction on the second mounting plate, is fixed with the second slide block being slidably matched with the second guide rail on the second portable plate.
The second driven unit comprise second drive-motor of body partial fixing on the second mounting plate, along the short transverse of body extend and synchronize with the rotating shaft of the second drive-motor connection the second drive lead screw and be fixed on the second portable plate the second feed screw nut with the second drive lead screw thread fit.
The upper end of sleeve pipe is fixed on the bottom of the second mounting plate removably.
Move liquid assembly and also comprise the first portable plate, piston rod is fixed on the first portable plate, is fixed with the first guide rail extending along bracket height direction on the first mounting plate, is fixed with the first slide block being slidably matched with the first guide rail on the first portable plate.
On the first mounting plate, be fixedly connected with train wheel bridge and lower plate, between train wheel bridge and lower plate, form the draw-in groove of a front openings, the upper end of transfer pipet stretches out and forms a flange being clamped in draw-in groove, on lower plate, be provided with a front openings accommodating groove, this accommodating groove forms a host cavity of accommodating transfer pipet away from its opening one end, accommodating groove sidewall is provided with an open holes near one end of its opening, in this installation, be provided with a top and be pressed on transfer pipet tube wall near the fastener of accommodating groove opening one side, in open holes, be provided with an elastic element, the elastic stress of this elastic element for providing one fastener is pressed towards the direction top near transfer pipet.
The first driven unit comprise first drive-motor of body partial fixing on the first mounting plate, along the short transverse of body extend and synchronize with the rotating shaft of the first drive-motor connection the first drive lead screw and be fixed on the first portable plate the first feed screw nut with the first drive lead screw thread fit.
The instrument of this extraction biologically active substance also includes a sample introduction assembly, this sample introduction assembly comprises the tail rod that extends along frame length direction, movable be socketed in adjustable shelf on tail rod, be fixed on liquid outlet on adjustable shelf, is communicated with liquid outlet flexible pipe, body with outside agent storing unit is fixed on the 3rd drive-motor in frame and by the 3rd drive-motor drive and along the pulley belt of frame length direction extension, adjustable shelf is fixedly connected on pulley belt.
Beneficial effect of the present invention is:
Than prior art, the present invention only on an instrument, can complete the operation of cracking, de-magnetic, washing, has improved the efficiency that biologically active substance extracts, and the sample after extracting has higher purity, for follow-up detection and research and development provide foundation accurately.Its de-magnetic process operation is simple, is conducive to apply.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is internal structure schematic diagram of the present invention;
Fig. 3 is the structural representation of worktable in Fig. 2;
Fig. 4 moves the structural representation of liquid assembly in Fig. 2;
Fig. 5 is that the A of Fig. 4 is to view;
Fig. 6 is the enlarged view at B place in Fig. 5;
Fig. 7 is the structural representation of separating assembly in Fig. 2;
Fig. 8 is the structural representation of sample introduction assembly in Fig. 2;
Wherein: 100, frame; 200, worktable; 210, pipe support; 211, finished product Reagent Tube station; 212, magnetic bead test tube station; 213, waste liquid tank; 214, culture test tube station; 215, pipettor station; 220, driving mechanism; 400, separating assembly; 410, the second mounting plate; 411, the second guide rail; 412, lower plate; 413, train wheel bridge; 420, the second portable plate; 421, the second slide block; 430, the second drive-motor; 431, the second drive lead screw; 440, magnetic bar; 450, sleeve pipe; 500, move liquid assembly; 510, the first mounting plate; 511, the first guide rail; 512, lower plate; 5121, spring; 5122, fastener; 5123, open holes; 513, train wheel bridge; 520, the first portable plate; 521, the first slide block; 522, lower plate; 523, train wheel bridge; 530, the first drive-motor; 531, the first drive lead screw; 540, piston rod; 550, transfer pipet; 551, flange; 600, sample introduction assembly; 610, the 3rd drive-motor; 611, pulley belt; 620, liquid outlet; 621, flexible pipe; 630, adjustable shelf; 631, tail rod.
Embodiment
Below, by reference to the accompanying drawings and embodiment, the present invention is described further:
Shown in Fig. 1-8, for a kind of instrument that extracts biologically active substance of the present invention, it comprises frame 100, worktable 200, moves liquid assembly 500 and separating assembly 400, wherein, frame 100 is as the installation basis of whole device, worktable 200, for placing various test tubes, moves liquid assembly 500 for transfer liquid when extracting active substance, and 400 of separating assemblies adopt the mode of absorption magnetic bead by the liquid separation producing after active substance and sample broken wall.Concrete structure of the present invention is as follows:
Worktable 200 is arranged on the bottom of frame 100, this worktable 200 comprises pipe support 210 and is arranged in pipe support 210 belows for driving pipe support 210 along the driving mechanism 220 of width (i.e. the Y-direction of the diagram) motion of frame 100, driving mechanism 220 is same as the prior art, can select the structure of driven by motor screw rod transmission, also can select driven by motor belt-driven structure.On worktable 200, be provided with a plurality of stations in order to place different test tubes, concrete is, on worktable 200, along the width of frame 100, set gradually to be from inside to outside useful on and place the finished product Reagent Tube station 211 of dressing up product test tube, for placing the magnetic bead test tube station 212 of dress magnetic bead liquid tube, for placing the culture test tube station 214 of dress sample, for placing the pipettor station 215 of transfer pipet 550, wherein, between magnetic bead test tube station 212 and culture test tube station 214, be also provided with a waste liquid tank 213, one end of waste liquid tank 213 forms a waste liquid outlet, in frame 100, be fixed with an intercepting basin being positioned under waste liquid outlet.
Move the top that liquid assembly 500 is positioned at worktable 200, the below of moving liquid assembly 500 coordinates transfer pipet 550, and can drive transfer pipet 550 to move up and down, thereby can being inserted on worktable 200, places in vitro by transfer pipet 550, to realize liquid transfer, liquid assimilating, to play the action that liquid mixes.This move liquid assembly 500 specifically comprise the first mounting plate 510, for drive the first mounting plate 510 along the first driver element (not shown) of the short transverse of frame 100 (i.e. the Z-direction of diagram) motion, be connected to transfer pipet 550 on the first mounting plate 510, be inserted in transfer pipet 550 and with the airtight piston rod coordinating 540 of inwall and first driven unit for driving piston rod 540 to move up and down with respect to transfer pipet 550 of transfer pipet 550.This first driven unit can be comprise first drive-motor 530 of body partial fixing on the first mounting plate 510, be synchronously connected in the rotating shaft of the first drive-motor 530 and the first drive lead screw 531 extending along the short transverse of frame 100 and with first feed screw nut's (not shown) of the first drive lead screw 531 thread fit, piston rod 540 is connected to the first feed screw nut.The first driven unit is for piston rod 540, make piston rod 540 and a structure of pumping liquid of transfer pipet 550 common compositions, it is different from the employing dosage pump structure in existing instrument, concrete is, in extracting biologically active substance, what sample, reaction solution etc. adopted conventionally is microdose, existing employing metering pump structure, conventionally be difficult to add accurately liquid, and adopt said structure of the present invention, the angle that can drive the first drive lead screws 531 turn over by accurate control the first drive-motor 530, controls the dosage of liquid accurately.For the ease of raising the efficiency, a plurality of piston rods 540 and corresponding a plurality of transfer pipets 550 can be set, the bottom that is fixed on the first mounting plate 510 side by side, the upper end of transfer pipet 550, movable one first portable plate 520 that is provided with on the first mounting plate 510, this first portable plate 520 can move with respect to the first mounting plate 510 along the short transverse of frame 100, the first above-mentioned feed screw nut is fixed on the first portable plate 520, the upper end of a plurality of piston rods 540 is fixed on the bottom of the first portable plate 520, on the first mounting plate 510, be provided with the first guide rail 511 extending along frame 100 short transverses, the back side of the first portable plate 520 is fixed with the first slide block 521 being slidably matched with the first guide rail 511.Replacing for the ease of piston rod 540, train wheel bridge 523 and lower plate 522 can be set in the bottom of the first portable plate 520, the radially extension edge of piston rod 540 upper ends is clamped between train wheel bridge 523 and lower plate 522, during installation, the extension edge of piston rod 540 upper ends is inserted by the opening between train wheel bridge 523 and lower plate 522, same, in the bottom of the first mounting plate 510, be fixed with train wheel bridge 513 and lower plate 512, between train wheel bridge 513 and lower plate 512, form the draw-in groove of a front openings, the upper end of transfer pipet 550 outwards forms a flange 551 radially extending, this flange 551 is clamped in the draw-in groove of the formation between train wheel bridge 513 and lower plate 512, on lower plate 512, be provided with the accommodating groove of a front openings, accommodating groove forms a host cavity of accommodating transfer pipet 550 away from one end of its opening, during installation, by after forward direction, the flange 551 of transfer pipet 550 upper ends being inserted in draw-in groove, make transfer pipet 550 be arranged in the host cavity that is partially embedded into accommodating groove inner side of flange 551 bottoms, in order to prevent that transfer pipet 550 and train wheel bridge 513 and lower plate 512 from departing from, the sidewall that can be positioned at accommodating groove on lower plate 512 arranges an open holes 5123 near one end of accommodating groove opening, open holes 5123 inside are provided with a fastener 5122, and the spring 5121 that is positioned at fastener 5122 inner sides, the elastic stress of spring 5121 for providing one fastener 5122 is pressed towards the direction top near transfer pipet 550, after transfer pipet 550 is installed in place, fastener 5122 tops are pressed on transfer pipet 550 tube walls near the surface of accommodating groove opening one side, when not being subject to, transfer pipet 550 is locked in fastener 5122 inner sides that are positioned at its both sides, when needs are dismantled, by transfer pipet 550, the opening by accommodating groove pulls, spring 5121 pressurizeds, transfer pipet 550 and lower plate 512 are departed from.When mounted, transfer pipet 550 is placed on pipettor station 215, the draw-in groove forming between train wheel bridge 513 and lower plate 512 aligns in short transverse with the flange 551 of transfer pipet 550, driving mechanism 220 drives pipe support 210 to move backward, the opening of the tube wall alignment accommodating groove of transfer pipet 550 is moved backward, during transfer pipet 550 tube wall contact card fastener 5122, spring 5121 pressurizeds, after transfer pipet 550 is in place, spring 5121 returns are locked at transfer pipet 550 in accommodating groove.Above-mentioned spring 5121 also can adopt the elastic elements such as elasticity blob of viscose to substitute.
Separating assembly 400 is positioned at the top of worktable 200, it comprises the second mounting plate 410, for the second driver element, the upper end that drives the second mounting plate 410 to move along the short transverse of frame 100, is fixed on sleeve pipe 450, magnetic bar 440 and second driven unit for driving magnetic bar 440 to move up and down with respect to sleeve pipe 450 of the second mounting plate 410 bottoms, the upper end opening of sleeve pipe 450, bottom are a cecum, magnetic bar 440 is inserted in sleeve pipe 450 by the upper end opening of sleeve pipe 450, and magnetic bar 440 and sleeve pipe 450 running fit.Magnetic bar 440 can be permanent magnet or electro-magnet, and it can adsorb the magnetic bead particles in magnetic bead liquid.In order to realize the separation of the active substance of a plurality of samples, a plurality of magnetic bars 440 can be set side by side and on the second mounting plate 410 side by side a plurality of sleeve pipes 450 are set, a plurality of magnetic bars 440 are fixed on the bottom of one second portable plate 420, being arranged on the one the second mounting plates 410 of these the second portable plate 420 activities, concrete is, the second guide rail 411 extending along frame 100 short transverses is set on the second mounting plate 410, on the second portable plate 420, be fixed with the second slide block 421 being slidably matched with the second guide rail 411, the second driven unit comprises second drive-motor 430 of body partial fixing on the second mounting plate 410, along the short transverse of frame 100, extend and be synchronously connected in the second drive lead screw 431 in the second drive-motor 430 rotating shafts, and with second feed screw nut's (not shown) of the second drive lead screw 431 thread fit, the second feed screw nut is fixed on the second portable plate 420.For the ease of dismounting, in the bottom of the second mounting plate 410, be provided with train wheel bridge 413 and lower plate 412, the radially extension edge of sleeve pipe 450 upper ends is clamped on train wheel bridge 413 and lower plate 412 by dismountable, certainly, sleeve pipe 450 and the second mounting plate 410 also can adopt other the mode that removably connects, as clamping, be spirally connected etc.
Except said structure, the instrument that the present invention extracts biologically active substance also includes a sample introduction assembly 600, this sample introduction assembly 600 comprises the tail rod 631 extending along frame 100 length directions (i.e. X-direction in diagram), movable be socketed in the adjustable shelf 630 on tail rod 631, be fixed on the liquid outlet 620 on adjustable shelf 630, the flexible pipe 621 that liquid outlet 620 is communicated with outside agent storing unit, body is fixed on the 3rd drive-motor 610 in frame 100, and the pulley belt 611 that drives and extend along frame 100 length directions by the 3rd drive-motor 610, adjustable shelf 630 is fixedly connected on pulley belt 611, start the 3rd drive-motor 610, can drive liquid outlet 620 to move on above-mentioned worktable 200 along the length direction of frame 100.
When the present invention works, the test tube that sample is housed is placed on culture test tube station 214, start instrument, driving mechanism 220 drives pipe support 210 to move, make on culture test tube station 214 to move to the position concordant with liquid outlet 620, liquid outlet 620 moves to the test tube top that sample is housed, in sample, add lysate, standing for some time, after sample cell broken wall, mobile pipe support 210, make the liquid assembly 500 that moves being unkitted by transfer pipet 550 be positioned at pipettor station 215 rearward positions, top, the first driver element drives the first mounting plate 510 to decline afterwards, the flange 551 of the transfer pipet 550 on pipettor station 215 and the draw-in groove between train wheel bridge 513 and lower plate 512 are horizontal, now, driving mechanism 220 drives pipe support 210 to move backward, make transfer pipet 550 be installed on the below of the first mounting plate 510, then, the first drive-motor 530 drives piston rod 540 be inserted in transfer pipet 550 downwards and discharge all gas in transfer pipet 550, afterwards, the first driver element drives transfer pipet 550 move to magnetic bead test tube station 212 tops and be inserted in the test tube that magnetic bead liquid is housed, the first drive-motor 530 drives piston rod 540 to move upward, magnetic bead liquid is sucked in transfer pipet 550, follow mobile pipe support 210, make transfer pipet 550 move to culture test tube station 214, the first drive-motor 530 drives piston rod 540 to move downward, make magnetic bead liquid join in the sample that scission reaction completes, the active substance of sample cell inside is adsorbed by magnetic bead, the second drive-motor 430 drives magnetic bar 440 to embed completely in sleeve pipe 450, and under the drive of the second driver element, the sleeve pipe 450 of having set magnetic bar 440 is inserted into the test tube that sample is housed, the magnetic bead that combines active substance is attracted to the surface of sleeve pipe 450 according to magnetic force, then, mobile pipe support 210, make the sleeve pipe 450 that adsorbs magnetic bead move to finished product Reagent Tube station 211 tops and sleeve pipe 450 is inserted in the test tube that TE reagent is housed of placing on finished product Reagent Tube station 211, active substance is dissolved in TE reagent, magnetic bar 440 is along with sleeve pipe 450 moves to waste liquid tank 213 tops, magnetic bar 440 departs from sleeve pipe 450, do not having under magnetic force use, magnetic bead departs from from sleeve pipe 450, complete de-magnetic process, afterwards, transfer pipet 550 is inserted in the test tube on finished product Reagent Tube station 211, piston rod 540 moves up and down repeatedly, wash action, in order to ensure the residual magnetic bead in finished product reagent middle part, above-mentioned de-magnetic moves with washing repeatedly, guarantee the purity of active substance, finally complete leaching process.
The present invention only on an instrument, can complete the operation of cracking, de-magnetic, washing, has improved the efficiency that biologically active substance extracts, and the sample after extracting has higher purity, for follow-up detection and research and development provide foundation accurately.Its de-magnetic process operation is simple, is conducive to apply.
To one skilled in the art, can make other various corresponding changes and deformation according to technical scheme described above and design, and within these all changes and deformation all should belong to the protection domain of the claims in the present invention.
Claims (8)
1. an instrument that extracts biologically active substance, is characterized in that, comprises,
Frame;
Be arranged on the worktable of frame bottom, on this worktable, be provided with for placing dress sample tube, dress magnetic bead liquid test tube and dressing up pipe support and the driving mechanism for driving pipe support to move along the width of frame of product test tube;
What be positioned at worktable top moves liquid assembly, this move liquid assembly comprise the first mounting plate, for the first driver element of driving the first mounting plate to move along the short transverse of frame, be connected to transfer pipet on the first mounting plate, be inserted in transfer pipet and with the airtight piston rod coordinating of inwall and first driven unit for driving piston rod to move up and down with respect to transfer pipet of transfer pipet;
Be arranged in above worktable and for the isolated separating assembly of the sample from cracking by biologically active substance, this separating assembly comprises the second mounting plate, for the second driver element of driving the second mounting plate to move along the short transverse of frame, be fixed on sleeve pipe, magnetic bar and the second driven unit for driving magnetic bar to move up and down with respect to sleeve pipe on the second mounting plate, the upper end opening of sleeve pipe, bottom are cecum, and in the mode of running fit, the upper end opening by sleeve pipe inserts in sleeve pipe magnetic bar.
2. the instrument of extraction biologically active substance as claimed in claim 1, it is characterized in that, separating assembly also comprises the second portable plate, magnetic bar is fixed on the second portable plate, on the second mounting plate, be fixed with the second guide rail extending along bracket height direction, on the second portable plate, be fixed with the second slide block being slidably matched with the second guide rail.
3. the instrument of extraction biologically active substance as claimed in claim 2, it is characterized in that, the second driven unit comprise second drive-motor of body partial fixing on the second mounting plate, along the short transverse of body extend and synchronize with the rotating shaft of the second drive-motor connection the second drive lead screw and be fixed on the second portable plate the second feed screw nut with the second drive lead screw thread fit.
4. the instrument of extraction biologically active substance as claimed in claim 2, is characterized in that, the upper end of sleeve pipe is fixed on the bottom of the second mounting plate removably.
5. the instrument of extraction biologically active substance as claimed in claim 1, it is characterized in that, move liquid assembly and also comprise the first portable plate, piston rod is fixed on the first portable plate, on the first mounting plate, be fixed with the first guide rail extending along bracket height direction, on the first portable plate, be fixed with the first slide block being slidably matched with the first guide rail.
6. the instrument of extraction biologically active substance as claimed in claim 5, it is characterized in that, on the first mounting plate, be fixedly connected with train wheel bridge and lower plate, between train wheel bridge and lower plate, form the draw-in groove of a front openings, the upper end of transfer pipet stretches out and forms a flange being clamped in draw-in groove, on lower plate, be provided with a front openings accommodating groove, this accommodating groove forms a host cavity of accommodating transfer pipet away from its opening one end, accommodating groove sidewall is provided with an open holes near one end of its opening, in this installation, be provided with a top and be pressed on transfer pipet tube wall near the fastener of accommodating groove opening one side, in open holes, be provided with an elastic element, the elastic stress of this elastic element for providing one fastener is pressed towards the direction top near transfer pipet.
7. the instrument of extraction biologically active substance as claimed in claim 5, it is characterized in that, the first driven unit comprise first drive-motor of body partial fixing on the first mounting plate, along the short transverse of body extend and synchronize with the rotating shaft of the first drive-motor connection the first drive lead screw and be fixed on the first portable plate the first feed screw nut with the first drive lead screw thread fit.
8. the instrument of extraction biologically active substance as claimed in claim 1, it is characterized in that, the instrument of this extraction biologically active substance also includes a sample introduction assembly, this sample introduction assembly comprises the tail rod that extends along frame length direction, movable be socketed in adjustable shelf on tail rod, be fixed on liquid outlet on adjustable shelf, is communicated with liquid outlet flexible pipe, body with outside agent storing unit is fixed on the 3rd drive-motor in frame and by the 3rd drive-motor drive and along the pulley belt of frame length direction extension, adjustable shelf is fixedly connected on pulley belt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410228543.9A CN103992940B (en) | 2014-05-27 | 2014-05-27 | A kind of instrument extracting biologically active substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410228543.9A CN103992940B (en) | 2014-05-27 | 2014-05-27 | A kind of instrument extracting biologically active substance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103992940A true CN103992940A (en) | 2014-08-20 |
| CN103992940B CN103992940B (en) | 2016-03-16 |
Family
ID=51307278
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410228543.9A Active CN103992940B (en) | 2014-05-27 | 2014-05-27 | A kind of instrument extracting biologically active substance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103992940B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450498A (en) * | 2014-12-16 | 2015-03-25 | 嘉兴凯实生物科技有限公司 | Nucleic acid extractor |
| CN104479991A (en) * | 2014-12-16 | 2015-04-01 | 嘉兴凯实生物科技有限公司 | Single gun device for nucleic acid extractor |
| CN104479992A (en) * | 2014-12-16 | 2015-04-01 | 嘉兴凯实生物科技有限公司 | Multi-channel pipette used in nucleic acid extraction instrument |
| WO2019017726A2 (en) | 2017-07-21 | 2019-01-24 | Seegene, Inc. | Modules for transferring magnetic beads, automated system comprising the same and method for nucleic acid extraction using the same |
| CN110088265A (en) * | 2016-12-30 | 2019-08-02 | 分子机械和工业公开股份有限公司 | Method and apparatus for separating and handling particle target |
| WO2019174471A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Pipetting mechanism |
| CN113820195A (en) * | 2021-11-25 | 2021-12-21 | 成都宜乐芯生物科技有限公司 | Multichannel parallel pretreatment device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060110296A1 (en) * | 1998-10-13 | 2006-05-25 | Precision Systems Science Co., Ltd. | Automatic separator/extractor and method of controlling same |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN203174084U (en) * | 2013-01-15 | 2013-09-04 | 北京金麦格生物技术有限公司 | Magnetic needle and casing mobile system for magnetic bar method extractor |
| CN203855572U (en) * | 2014-05-27 | 2014-10-01 | 广州冠科生物科技有限公司 | Instrument for extracting active substance of organisms |
-
2014
- 2014-05-27 CN CN201410228543.9A patent/CN103992940B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060110296A1 (en) * | 1998-10-13 | 2006-05-25 | Precision Systems Science Co., Ltd. | Automatic separator/extractor and method of controlling same |
| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN203174084U (en) * | 2013-01-15 | 2013-09-04 | 北京金麦格生物技术有限公司 | Magnetic needle and casing mobile system for magnetic bar method extractor |
| CN203855572U (en) * | 2014-05-27 | 2014-10-01 | 广州冠科生物科技有限公司 | Instrument for extracting active substance of organisms |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104479991A (en) * | 2014-12-16 | 2015-04-01 | 嘉兴凯实生物科技有限公司 | Single gun device for nucleic acid extractor |
| CN104479992A (en) * | 2014-12-16 | 2015-04-01 | 嘉兴凯实生物科技有限公司 | Multi-channel pipette used in nucleic acid extraction instrument |
| CN104479992B (en) * | 2014-12-16 | 2016-07-06 | 嘉兴凯实生物科技有限公司 | A kind of instrument for extracting nucleic acid volley of rifle fire device |
| CN104450498A (en) * | 2014-12-16 | 2015-03-25 | 嘉兴凯实生物科技有限公司 | Nucleic acid extractor |
| CN110088265B (en) * | 2016-12-30 | 2022-06-10 | 分子机械和工业公开股份有限公司 | Method and apparatus for separating and processing particulate targets |
| CN110088265A (en) * | 2016-12-30 | 2019-08-02 | 分子机械和工业公开股份有限公司 | Method and apparatus for separating and handling particle target |
| WO2019017726A2 (en) | 2017-07-21 | 2019-01-24 | Seegene, Inc. | Modules for transferring magnetic beads, automated system comprising the same and method for nucleic acid extraction using the same |
| CN110945363A (en) * | 2017-07-21 | 2020-03-31 | Seegene株式会社 | Module for transferring magnetic beads, automated system comprising the module, and method for extracting nucleic acid using the module |
| CN110945363B (en) * | 2017-07-21 | 2024-04-16 | Seegene株式会社 | A module for transferring magnetic beads, an automated system including the module, and a method of extracting nucleic acid using the module |
| US11674971B2 (en) | 2017-07-21 | 2023-06-13 | Seegene, Inc. | Modules for transferring magnetic beads, automated system comprising the same and method for nucleic acid extraction using the same |
| WO2019174471A1 (en) * | 2018-03-13 | 2019-09-19 | 武汉医蒂生物科技有限公司 | Pipetting mechanism |
| EP4187250A1 (en) * | 2021-11-25 | 2023-05-31 | Chengdu Illumaxbio Technology Co., Ltd. | Multi-channel parallel pretreatment device |
| CN113820195B (en) * | 2021-11-25 | 2022-03-08 | 成都宜乐芯生物科技有限公司 | Multichannel parallel pretreatment device |
| CN113820195A (en) * | 2021-11-25 | 2021-12-21 | 成都宜乐芯生物科技有限公司 | Multichannel parallel pretreatment device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103992940B (en) | 2016-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103992940A (en) | Instrument for extracting bioactive substance | |
| US10961491B1 (en) | Magnetic bead-based nucleic acid extraction apparatus and use method | |
| CN103789198B (en) | Full automatic instrument for extracting nucleic acid | |
| CN203855572U (en) | Instrument for extracting active substance of organisms | |
| CN101596469B (en) | Multi-channel loading device | |
| CN108048302B (en) | Full-automatic nucleic acid extractor | |
| CN103305412A (en) | Full-automatic nucleic acid extraction machine | |
| CN208964916U (en) | A kind of magnetic beads for purifying instrument of enrichment method | |
| CN203923174U (en) | A kind of nucleic acid extraction evenly mixing device | |
| CN102366725B (en) | Automatic sampling gun | |
| CN104059848A (en) | Uniform mixing device for nucleic acid extraction | |
| CN107760676B (en) | Full-automatic extraction instrument for paramagnetic particles | |
| CN208667685U (en) | A kind of nucleic acid-extracting apparatus | |
| CN103041738A (en) | Oscillator capable of rapidly moving | |
| CN201493121U (en) | Multi-channel sample adding device | |
| CN103575571A (en) | Multi-channel full-automatic solid-phase extraction device | |
| CN104263633A (en) | Magnetic-separation-based high-flux biological sample treatment device | |
| CN205856483U (en) | A kind of multichannel nucleic acid automatic extracting instrument | |
| CN2925616Y (en) | Universal magnetic separating stand | |
| CN211112037U (en) | High-flux nucleic acid extraction system | |
| CN105021835A (en) | Magnetically driven biological sample treatment device and treatment method thereof | |
| CN202182890U (en) | Y-direction driving mechanism for automatic sample-adding guns | |
| CN201020402Y (en) | Detachable utility type magnetic separating rack | |
| CN203881598U (en) | Sample smearing processing device | |
| CN108315243B (en) | Automatic sample adding system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |