CN103992159A - Full-element compound microbial fertilizer and preparation method and application thereof - Google Patents

Full-element compound microbial fertilizer and preparation method and application thereof Download PDF

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CN103992159A
CN103992159A CN201410114967.2A CN201410114967A CN103992159A CN 103992159 A CN103992159 A CN 103992159A CN 201410114967 A CN201410114967 A CN 201410114967A CN 103992159 A CN103992159 A CN 103992159A
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fertilizer
composite microbiological
unit entirely
sqr9
total
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CN103992159B (en
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沈其荣
梁晓琳
李�荣
黄蓉
文春燕
张瑞福
沈标
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Nanjing Agricultural University
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Nanjing Agricultural University
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention provides a full-element compound microbial fertilizer and a preparation method and application thereof, and belongs to agricultural high-tech production technology. An organic fertilizer rich in Bacillus amyloliquefaciens SQR-9 (preservation number of CGMCC NO.5808) is added with 14.8% (dry weight) of ammonium sulfate to reach the total nutrient in the compound microbial fertilizer to 10%, and the mixture is granulated by a disc to obtain the fertilizer. The full-element compound microbial fertilizer (granulation type) contains total nutrients satisfying the relation of total nitrogen(TN) + total phosphorus (P2O5) + total potassium (K2O)= 0%, and after 6 months of storage, the number of functional bacteria is no less than 2*107 CFU / g of the dry weight of the fertilizer. The novel synergistic fertilizer has the quick result of fertilizer, long-acting property of organic fertilizer and synergistic function of biofertilizer, and has good growth promoting effect, low production cost and convenience for application, and can be applied to grain crops and vegetables, etc.

Description

A kind of complete first composite microbiological fertilizer and its preparation method and application
Technical field
The invention belongs to biological fertilizer field, relate to one " unit entirely " composite microbiological fertilizer and its preparation method and application.
Background technology
Some developed countries are the twenties in last century, China starts to enter the agricultural modernization stage the sixties in last century, take the conventional agriculture modernization development pattern of " high investment, high production, high pollution ", level of agricultural production is increased substantially, but also cause the extreme consumption of resource simultaneously, the havoc of ecotope and pollution.Along with the development of chemical fertilizer industry and the increase of agrochemical amount of application, the output of farm crop has had very large growth.Also caused a large amount of problems when making huge contribution but chemical fertilizer is Agriculture Production: (1) soil fertility decline (Chen Shiping. Chinese fertilizer structure modest proposal [J]. ten earth fertilizer, 1984 (4): 21～24.); (2) soil physico-chemical property become bad (Wang Jianfei, Xing Suzhi. negative effect and the Preventing Countermeasures [J] thereof of chemical fertilizer used in farmland. agriculture environmental protection, 1998,17 (1): 40,--43); (3) biological activity declines; (4) in soil, macroelement and micro-balance are destroyed; (5) crop failure, disease and pest serious (Kevin G, Tiller, 1989; Nishizaura N S Mor, 1977); (6) chemical fertilizer utilization ratio is low, pollution of ecological environment (Xia Rongji. soil and fertilizer rudimentary knowledge [M]. Beijing: agriculture press, 1981.), aggravation Greenhouse effect, polluted underground water (Liu little Lan, Li Shiqing. the nitrogen in soil and environment [J]. agricultural research in the arid areas, 1998,16 (4): 36～43.); (7) affect agricultural product quality (Liu shares a bitter hatred for Hu Chengxiao, Deng Boer. the impact [J] of nitrogen level on quality of vegetable. ten earth fertilizer, 1996 (3): 34～36.) etc.In China due to long-term loose amount and unreasonable using, fertilizer takes effect slowly, uses that area and consumption wretched insufficiency and bio-bacterial manure efficiency are low, application small scale, without Fertilizer application features such as region specific aim, marketing difficulty are large, cause China's Soil structure to be seriously damaged, organic content sharply declines, structure of soil microbial community serious unbalance, thereby cause agricultural output and quality significantly to decline and a series of distinct issues such as utilization rate of fertilizer reduced year by year.
Meanwhile, the fertilizer that a large amount of agricultural wastes such as agricultural crop straw and feces of livestock and poultry are made, due to low fertilizer efficiency and added value, causes usable floor area and usage quantity to be seriously obstructed, and causes ample resources waste and serious ecological environmental pollution.Therefore, can integrate that chemical fertilizer is quick-acting, fertilizer is long-acting and the new-type fertilizer production technology of bio-fertilizer synergy by its Industry Promotion in the urgent need to development, and then raising per unit area yield, complete the target to the newly-increased 1,000 hundred million jin of grain-production abilities of the year two thousand twenty that country establishes.
Summary of the invention
The object of the invention is for long-term loose amount and unreasonable using, fertilizer takes effect slowly, uses that area and consumption wretched insufficiency and bio-bacterial manure efficiency are low, application small scale, without features such as region specific aim, marketing difficulty are large, the problem that simultaneously inconvenient mechanize is used for powder fertilizer, develops " unit entirely " composite microbiological fertilizer (granulation type) of the long-lasting and bio-bacterial manure synergy of the quick-acting, the fertilizer that integrate inorganic fertilizer.
Another object of the present invention is to provide the preparation method of " unit entirely " composite microbiological fertilizer.
Another object of the present invention is to provide the application of " unit entirely " composite microbiological fertilizer.
Object of the present invention can be achieved through the following technical solutions:
A kind of " unit entirely " composite microbiological fertilizer, mainly prepares by following steps:
1) after ammonium sulfate pulverizing, mistake 18 mesh sieves, add in biological organic fertilizer and mix to obtain mixed fertilizer, making mixed fertilizer total nutrient is 10%;
2) utilize granulating disc to carry out granulation to mixed fertilizer, after granulating, under temperature is no more than the condition of 50 DEG C, the water content of mixed fertilizer grain is down to below 20%, " unit entirely " composite microbiological fertilizer gets product.
Wherein, described biological organic fertilizer is that 60% decomposed cow dung compost and 40% dish dregs of rice compost are evenly mixed, inoculation preserving number be CGMCC NO.5808 bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SQR ?9 fermented liquid solid state fermentations form; Described biological organic fertilizer is containing organic >=45%, total nutrient >=5%, water content≤30%, pH value 6.5 ?8.5.
Described bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SQR ?9 fermented liquids be that bacterial strain SQR9 is inoculated in PDA nutrient solution, carry out liquid fermenting production, the condition of fermentative production is: 30～37 DEG C of culture temperature scopes, stirring velocity is 170～180 revs/min, the fermentation middle and later periods forms gemma, makes in fermented liquid containing bacterium or gemma amount>=1 × 10 9individual/ml; PDA nutrient solution compound method used is, is cut into small pieces to be put in water boils with 200g potato after peeling, and boils 30min after boiling, adds after filtering 20g common sucrose in filtrate, is settled to 1000ml, pH value be adjusted to 7.0 ?7.6,121 DEG C of sterilizing 20min.
Described solid fermentation is to carry out solid fermentation in the cow dung compost mixture after described bacterial strain SQR9 fermented liquid is inoculated to the dish dregs of rice and become thoroughly decomposed, in SOLID ORGANIC material per ton, add bacterial strain SQR9 fermented liquid 40～60L, turning every day 1～2 time in solid fermentation process, make solid fermentation temperature be no more than 60 DEG C, ferment 6 ?after 7 days, finish, growth-promoting bacterial content can reach 2 × 10 8more than CFU/g fertilizer dry weight, under the last condition that is no more than 60 DEG C in temperature, the water content of microbial organic fertilizer is evaporated to below 30%, pulverizes, cross 20 mesh sieves; This fertilizer quality of organic matter is than content>=45%, and organonitrogen quality is 2 ?3% than content.
Described " unit entirely " composite microbiological fertilizer, taking biological organic fertilizer dry weight as benchmark, the addition of ammonium sulfate is biological organic fertilizer 10～16%.
Described " unit entirely " composite microbiological fertilizer is preserved effective efficiency viable count>=2 × 10 after 180 days 7cFU/g fertilizer dry weight, total nitrogen content is 4 ?6%, and total npk nutrient is 10%, and finished particle size is diameter 1 ?2mm, water content≤20%.
Utilize chemical fertilizer and biological organic fertilizer to produce the technique of " unit entirely " complex microorganism granulation fertilizer, comprising:
1) be that rhizosphere growth-promoting bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SQR9 of CGMCC NO.5808 is inoculated in PDA nutrient solution by preserving number, carry out liquid fermenting production, the condition of fermentative production is: 30～37 DEG C of culture temperature scopes, stirring velocity is 170～180 revs/min, the fermentation middle and later periods forms gemma, makes in fermented liquid containing bacterium or gemma amount>=1 × 10 9individual/ml; PDA nutrient solution compound method used is, is cut into small pieces to be put in water boils with 200g potato after peeling, and boils 30min after boiling, adds after filtering 20g common sucrose in filtrate, is settled to 1000ml, pH value be adjusted to 7.0 ?7.6,121 DEG C of sterilizing 20min;
2) be that 60% cow dung and 40% dish dregs of rice compost evenly mix by mass ratio;
3) growth-promoting bacterium SQR9 fermented liquid is inoculated the dish dregs of rice and become thoroughly decomposed after cow dung compost mixture in carry out solid fermentation, in SOLID ORGANIC material per ton, add growth-promoting bacterium liquid 40～60L, turning every day 1～2 time in solid fermentation process, make solid fermentation temperature be no more than 60 DEG C, ferment 6 ?after 7 days, finish, growth-promoting bacterial content can reach 2 × 10 8more than CFU/g fertilizer dry weight, under the last condition that is no more than 60 DEG C in temperature, the water content of microbial organic fertilizer is evaporated to below 30%, this fertilizer quality of organic matter is than content>=45%, and organonitrogen quality is 2 ?3% than content.Pulverize with disintegrating machine, cross 20 mesh sieves;
3) determine the amount of the ammonium sulfate adding according to the concrete nutrient of above-mentioned biological organic fertilizer raw material, it is mixed after total nutrient be 10%.Ammonium sulfate need pulverize after with sieve after biological organic fertilizer raw material mix;
4) utilize granulating disc according to common process by mixture granulation, after granulating, under temperature is no more than the condition of 50 DEG C, the water content of microbial organic fertilizer is dried to below 20%, packaging is dispatched from the factory and is finished product " unit entirely " complex microorganism granulation fertilizer; Its organonitrogen quality is 4 ?6% than content, and water content mass ratio is below 20%.
Useful viable count>=2 × 10 in " unit entirely " composite microbiological fertilizer of producing with above-mentioned Recipe 7cFU/g(fertilizer dry weight), total nitrogen content is 4 ?6%, total npk nutrient is 10%, organic content>=30%.This products material shatters and sieves, finished particle size be diameter 1 ?2mm, water content mass ratio is below 20%.
The application of " unit entirely " composite microbiological fertilizer of the present invention aspect promotion crop growth and volume increase, the preferably application aspect promotion eggplant or tomato growth and volume increase.
Beneficial effect
Use " unit entirely " composite microbiological fertilizer that this invention is produced to integrate the quick-acting of inorganic fertilizer, the long-lasting and bio-bacterial manure synergy of fertilizer, fertilizer efficiency discharges steadily, growth-promoting is effective, is applicable to the cash crop planting area big area such as vegetables, fruit tree and uses.The present invention is for preserving the ecological environment, and the protection people's is healthy, and the added value that improves agricultural-food has great importance.
Product tool compared with product in the market has the following advantages:
1) the present invention is using the common fertilizer of decomposed cow dung and dish cake as composite fertilizer material, secondary solid fermentation rhizosphere growth-promoting Bacillus amyloliquefaciens SQR9, develop the good biological organic fertilizer of growth-promoting effect, after air-dry pulverizing, adding a certain amount of ammonium sulfate makes its total nutrient reach 10%, utilize the granulation of disc granulation technique, this formula and technique can significantly reduce the cost of " unit entirely " composite microbiological fertilizer." unit entirely " composite microbiological fertilizer is a kind of " efficiently, safety, environment-friendly type " fertilizer.It combines bio-fertilizer, fertilizer, three kinds of fertilizer of inorganic fertilizer, and the quick-acting effects of steady effect, long-acting and the inorganic fertilizer of the synergy of bio-fertilizer, short effect effect and fertilizer are mutually combined, anxious slow Ji mutually, and nutrient is comprehensive.Both ensured the indispensable nutritive ingredient of plant growth, improve the soil again, growth-promoting, disease-resistant, improve the ecological environment, to produce high quality agricultural products, alleviating environmental pollution has great role.
2) in " unit entirely " composite microbiological fertilizer, the survival of microorganism is a very important problem, the rhizosphere growth-promoting Bacillus amyloliquefaciens SQR9 good salt tolerance that this patent uses, vitality is strong, and it has anti-yellowing cucurbit wilt, effect of wide spectrum growth-promoting, directly, with a certain amount of ammonium sulfate mixing granulation, air-dry rear storage met living bacteria count>=2 × 10 after 180 days 7cFU/g(fertilizer dry weight).Directly mixed granulation process greatly reduces the production cost of " unit entirely " composite microbiological fertilizer.
3) " unit entirely " composite microbiological fertilizer after granulation easily stores, and outward appearance is good, uses conveniently.
4) simultaneously abundant amino acid resource can promote plant growth in fertilizer, the physiological regulation function of fertilizer collection microorganism uniqueness, and the high efficiency of inorganic fertilizer and fertilizer long-lasting in one, reaches the object of breeding combination and agricultural sustainable development.Test-results shows, uses after this product on vegetable sprout term, can significantly promote the growth of crop, and effect of increasing production is extremely remarkable.
The present invention " unit entirely " composite microbiological fertilizer, has the activating soil of biological bacteria, the function of conversion soil nutrient concurrently, organic fertilizing soil, the improvement feature of soil and the quick-acting functions of inorganic nutrients; Can increase the nutrition content of Nitrogen In Soils element, organic matter, titanium pigment, soluble potassium, increase beneficial microorganism population and quantity in soil, can strengthen disease-resistant, drought-resistant ability and the metabolism of crop simultaneously; To improving crop yield, improve crop quality, increase peasant economy income and have positive effect.The research and development of novel all element compound fertilizer simultaneously can increase considerably the added value of fertilizer, thereby further promote the development of such enterprise, go deep into resource utilization organic waste protection of the environment." unit entirely " composite microbiological fertilizer, on conventional bacterial manure and inorganic fertilizer basis, increases organism pretreatment technology or adds the special complex process under condition in microorganism, strengthens organic proportion, outstanding microorganism and organic effect.After incorporation of fertilizerin the soil, a large amount of probioticss are first impressions are strongest, and suppress harmful bacteria propagation, keep soil micro-ecosystem balance, and can strengthen biological activity of soil and biochemical activity, improve crop rhizosphere environment, play diseases prevention effect in strong sprout.The organic matter adding is directly for crop provides organic nutriment, for microorganism provides effective carrier, and with microorganism together with common activating rhizosphere soil, improve soil physico-chemical property, make the interior inorganic nutrients Stable Release of complex body.Inorganic nutrients provides rational N, P, K three elements proportioning for different soils and Different Crop, adjusts the C/N ratio of complex body, makes it to be conducive to the breeding growth of microorganism and the performance of beneficial functions.And produce " unit entirely " composite microbiological fertilizer and can utilize the specific function of microorganism, agricultural organic waste is carried out to harmless treatment, turn waste into wealth, resource is fully used, environment improves and purifies.From current agriculture production actual needs, than factors considerations such as valence effect, fertilising custom, economic benefit and environment protection, it is the main direction of following fertilizer development that this three is integrated, and can produce great pushing effect to agriculture production.
Brief description of the drawings
Fig. 1 " unit entirely " composite microbiological fertilizer particle
Fig. 2 " unit entirely " composite microbiological fertilizer staging life viable count changes
Biomaterial preservation information
SQR9, Classification And Nomenclature is bacillus amyloliquefaciens Bacillus amyloliquefaciens, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preservation date is on February 27th, 2012, and preserving number is CGMCC NO.5808.
Embodiment
Embodiment 1
1, the common fertilizer of decomposed cow dung and dish cake provide
Cow dung and the dish dregs of rice are bought and are obtained by market.
2, the production of function yeast microbial inoculum
Rhizosphere growth-promoting bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SQR9 that is CGMCC NO.5808 by preserving number is inoculated in PDA nutrient solution, carry out liquid fermenting production, the condition of fermentative production is: 30～37 DEG C of culture temperature scopes, stirring velocity is 170～180 revs/min, the fermentation middle and later periods forms gemma, makes in fermented liquid containing bacterium or gemma amount>=1 × 10 9individual/ml; PDA nutrient solution compound method used is, is cut into small pieces to be put in water boils with 200g potato after peeling, and boils 30min after boiling, adds after filtering 20g common sucrose in filtrate, is settled to 1000ml, pH value be adjusted to 7.0 ?7.6,121 DEG C of sterilizing 20min.
3, solid fermentation generates biological organic fertilizer
60% decomposed cow dung compost and the 40% dish dregs of rice are evenly mixed, by growth-promoting bacterium SQR9 access hybrid reactor, in SOLID ORGANIC material per ton, add growth-promoting bacterium liquid 50L to carry out solid fermentation, turning every day 1 time, make solid fermentation temperature be no more than 60 DEG C, ferment and finish after 7 days.
In fermentation ends artifact fertilizer, growth-promoting bacterium SQR9 quantity reaches 5 × 10 8cFU/g(dry weight), quality of organic matter is than content>=45%, water content mass ratio 25 ?30%, total nitrogen content is that 2 ?3%(90% are organonitrogen above), total npk nutrient is>=5%.
4, a certain amount of ammonium sulfate production of proportioning " unit entirely " composite microbiological fertilizer
Utilize the method that organic fertilizer standard (NY525 ?2012) provides to measure total npk nutrient content in biological organic fertilizer, the results are shown in Table 1.Air-dry 20 mesh sieves that shattered of biological organic fertilizer mix to obtain mixed fertilizer with the ammonium sulfate of pulverizing, and the addition of ammonium sulfate is the 14.8wt% of biological organic fertilizer dry weight, and mixed fertilizer total nutrient is 10%.Utilize granulating disc granulation, particle air-dry water content mass ratio that makes under the condition that is no more than 50 DEG C is low to moderate below 20%.Sub-sieve, finished particle diameter 1 ?2mm, packaging is finished product " full unit " composite microbiological fertilizer (Fig. 1).
Table 1 biological organic fertilizer raw material and composite microbiological fertilizer nutrient
Embodiment 2 warmhouse booth tests
1. " unit entirely " composite microbiological fertilizer land for growing field crops effect on eggplant
Establish altogether a processing of three contrasts, " unit entirely " composite microbiological fertilizer (BOF) of processing interpolation the present invention development imposes appropriate chemical fertilizer, contrasts as (the CK that do not apply fertilizer 1) and add respectively the chemical fertilizer (CK of conventional amount used 2) and deactivation " full unit " composite microbiological fertilizer (AOF) impose appropriate chemical fertilizer, test kind is black galloping eggplant, except not applying fertilizer contrast, other nutrients such as processing grade.3 communities of each processing, each zone leader 8m, wide 2.5 meters, area 20m 2, 3kg(dry weight is used in every community) and " unit entirely " composite microbiological fertilizer, Routine Management.Results of regular determination eggplant growth index.The impact of different treatment on eggplant biological character and structure of output, as shown in table 2, on plant height, " unit entirely " composite microbiological fertilizer (BOF) is processed and is significantly better than other processing (P=0.05), deactivation fertilizer processing (AOF) and chemical fertilizer (CK 2) there was no significant difference but be significantly better than (the CK that do not apply fertilizer between processing 1) process.On stem is thick, BOF processes (having maximum value) and processes there was no significant difference with AOF, but is all significantly higher than CK 2and CK 1.SPAD value, on soluble proteins and soluble sugar content, difference that between all processing, there are no significant.BOF processes and has maximum single fruit weight, is respectively AOF, CK subsequently 2and CK 1.The result of theoretical per mu yield aspect is BOF>AOF>CK 2>CK 1, and BOF processing is significantly higher than CK 2and CK 1.Show that the application of NEW TYPE OF COMPOSITE microorganism granulation fertilizer (BOF) can significantly improve biological character and the structure of output of crop.
Different treatment is on the impact of output in table 3, and " unit entirely " composite microbiological fertilizer is processed than the volume increase 860kg/ mu that do not apply fertilizer, and amount of increase in production reaches 31.2%; Inactivation treatment is than the volume increase 653kg/ mu that do not apply fertilizer, and amount of increase in production reaches 23.7%.Meanwhile, " unit entirely " complex microorganism granulation fertilizer is processed than single and is increased production to fertilize 273kg/ mu, and amount of increase in production reaches 8.17%; Inactivation treatment is increased production 66kg/ mu to fertilize than single, and amount of increase in production reaches 1.97%." unit entirely " composite microbiological fertilizer is processed the mu than inactivation treatment volume increase 207kg/, and amount of increase in production reaches 6.07%.
Yield result is carried out to variance analysis discovery (table 4, table 5), F>F between processing 0.01>F 0.05; And F<F between repeating 0.05<F 0.01, significantly (P=0.01) of otherness between processing, in repeating, under P=0.05 and P=0.01, difference is all not remarkable.Meanwhile, the otherness between relatively processing and between repeating is found, F>F 0.01>F 0.05, that is to say, under P=0.05 and P=0.01, the variation between processing is greater than the variation between repetition significantly, and different treatment has different effect for eggplant yield.Between output is processed, otherness significance analysis is found: " unit entirely " composite microbiological fertilizer is compared with not applying fertilizer to contrast with chemical fertilizer, has significant effect of increasing production (P=0.05), and output is higher than the processing of deactivation compound manure simultaneously.
Table 2 " unit entirely " impact of composite microbiological fertilizer on eggplant biological character and structure of output
The impact of table 3 different treatment on eggplant yield result
Table 4 yield result analysis of variance table
The Notable difference test (LSR method) of table 5 output
5.2 " unit entirely " composite microbiological fertilizer land for growing field crops effect on tomato
Test design and the same eggplant of fertilising scheme, test kind is red hat of century.The impact that different treatment is learned proterties and structure of output to tomato biological is as shown in table 6 and table 7, and on plant height, " unit entirely " composite microbiological fertilizer (BOF) is processed and is significantly better than chemical fertilizer contrast (CK 2), fertilizer treatment (CK not 1) and deactivation fertilizer processing (AOF) (P=0.05).On stem is thick, BOF processes and is significantly better than chemical fertilizer contrast and fertilizer treatment not.In SPAD value, VC content and sugar-acid ratio, difference that between all processing, there are no significant.BOF processes and has maximum single fruit weight, is respectively AOF, CK subsequently 2and CK 1.The result of theoretical per mu yield aspect is BOF>AOF>CK 2>CK 1, and BOF processing is significantly higher than AOF, CK 2and CK 1.The application that shows " unit entirely " composite microbiological fertilizer (BOF) can significantly improve biological character and the structure of output of crop.
Different treatment is on the impact of output in table 8, and on the impact of output as shown in Table 3, " unit entirely " compound microbial fertilizer is than the volume increase 1394.03kg/ mu that do not apply fertilizer for different treatment, and amount of increase in production reaches 37.73%; Increase production to fertilize 473.57kg/ mu than single, amount of increase in production reaches 10.26%; Process volume increase 386.86kg/ mu than deactivation fertilizer, amount of increase in production reaches 8.23%.
Output statistics analytical results is in table 9 and 10, F>F between processing 0.01>F 0.05, F<F between repetition 0.05<F 0.01, significantly (P=0.01) of otherness between processing, in repeating, under P=0.05 and P=0.01, difference is all not remarkable.Meanwhile, the otherness between relatively processing and between repeating is found, F>F 0.01>F 0.05, that is to say, under P=0.05 and P=0.01, the variation between processing is significantly greater than the variation between repetition, and different treatment is to have different effect for tomato yield.Between output is processed, otherness significance analysis is found: " unit entirely " composite microbiological fertilizer all has significant difference with other processing under P=5%; And inactivation treatment, chemical fertilizer all do not have significant difference at 5% time between processing, but compare and all there is significant difference with the processing of not applying fertilizer.
Table 6 " unit entirely " composite microbiological fertilizer is learned the impact of proterties and structure of output on tomato biological
Table 7 " unit entirely " impact of composite microbiological fertilizer on tomato yield structure
The impact of table 8 different treatment on tomato yield result
Table 9 yield result analysis of variance table
The Notable difference test (LSR method) of table 10 output
Mixing granulation test and storage are tested and are shown, the composition and engineering of this research can assurance function bacterium number of viable.By the mixture granulation after sieving, control material amount of water and granulation time with granulating disc, fertilizer after granulation is air-dry to water content mass ratio below 20%.A part is for land for growing field crops fertilising, and a part is placed on sealing in plastics bag and keeps in Dark Place, and counts once at set intervals with method of dilution butteron on plate, and result as shown in Figure 2.
Warmhouse booth test-results entirety shows, " full unit " composite microbiological fertilizer (granulation type) use the output that can effectively increase facility eggplant and tomato, compare the contrast that contrasts and do not apply fertilizer with deactivation fertilizer, chemical fertilizer, eggplant has increased production respectively 6.07%, 8.17% and 31.2%, and tomato has increased production respectively 8.23%, 10.26% and 37.73%.Show synergy and the one of the quick-acting and functional microorganism of long-acting, the chemical fertilizer of this patent product collection fertilizer.Therefore, " unit entirely " complex microorganism granulation fertilizer material formula and the technique of this patent research and development have very strong practicality.

Claims (9)

1. " unit entirely " composite microbiological fertilizer, is characterized in that mainly preparing by following steps:
1) after ammonium sulfate pulverizing, mistake 18 mesh sieves, add in biological organic fertilizer and mix to obtain mixed fertilizer, making mixed fertilizer total nutrient is 10%;
2) utilize granulating disc to carry out granulation to mixed fertilizer, after granulating, under temperature is no more than the condition of 50 DEG C, the water content of mixed fertilizer grain is down to below 20%, " unit entirely " composite microbiological fertilizer gets product.
2. " unit entirely " according to claim 1 composite microbiological fertilizer, is characterized in that described biological organic fertilizer is first 60% decomposed cow dung compost and the 40% dish dregs of rice evenly to be mixed; By preserving number be CGMCC NO.5808 growth-promoting bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SQR ?9 fermented liquids inoculate and in the dish dregs of rice and decomposed cow dung compost mixture, carry out solid fermentation and form; Described biological organic fertilizer is containing organic >=45%, total nutrient >=5%, water content≤30%, pH value 6.5 ?8.5.
3. " unit entirely " according to claim 2 composite microbiological fertilizer, it is characterized in that described bacillus amyloliquefaciens SQR ?9 fermented liquids be that bacillus amyloliquefaciens SQR9 is inoculated in PDA nutrient solution, carry out liquid fermenting production, the condition of fermentative production is: 30～37 DEG C of culture temperature scopes, stirring velocity is 170～180 revs/min, the fermentation middle and later periods forms gemma, makes in fermented liquid containing bacterium or gemma amount>=1 × 10 9individual/ml.
4. " unit entirely " according to claim 3 composite microbiological fertilizer, it is characterized in that described solid fermentation is that described SQR9 fermented liquid is inoculated in the dish dregs of rice and decomposed cow dung compost mixture and carried out solid fermentation, in SOLID ORGANIC material per ton, add SQR9 fermented liquid 40～60L, turning every day 1～2 time in solid fermentation process, make solid fermentation temperature be no more than 60 DEG C, ferment 6 ?after 7 days, finish, SQR9 content reaches 2 × 10 8more than CFU/g fertilizer dry weight, under the last condition that is no more than 60 DEG C in temperature, the water content of microbial organic fertilizer is evaporated to below 30%, pulverizes, cross 20 mesh sieves; Quality of organic matter content>=45% in described biological organic fertilizer, organonitrogen mass content be 2 ?3%.
5. according to " full unit " composite microbiological fertilizer described in any one in claim 1～4, it is characterized in that taking biological organic fertilizer dry weight as benchmark, the addition of ammonium sulfate is biological organic fertilizer 10～16%, and making mixed fertilizer total nutrient is 10%.
6. " unit entirely " according to claim 1 composite microbiological fertilizer, after 180 days effective efficiency viable count>=2 × 10 to it is characterized in that described " unit entirely " composite microbiological fertilizer preservation 7cFU/g fertilizer dry weight, total nitrogen content is 4 ?6%, and total npk nutrient is 10%, and finished particle size is diameter 1 ?2mm, water content≤20%.
7. the preparation method of " unit entirely " claimed in claim 1 composite microbiological fertilizer, is characterized in that comprising following steps:
1) be that the bacillus amyloliquefaciens SQR9 of CGMCC NO.5808 is inoculated in PDA nutrient solution by preserving number, carry out liquid fermenting production, fermentation condition is: 30～37 DEG C of culture temperature, 170～180 revs/min of stirring velocitys, the fermentation middle and later periods forms gemma, makes in fermented liquid containing bacterium or gemma amount>=1 × 10 9individual/ml;
2) 60% cow dung and 40% dish dregs of rice compost are evenly mixed;
3) the bacterial strain SQR9 fermented liquid of being prepared by step 1) is inoculated in the dish dregs of rice and decomposed cow dung compost mixture and is carried out solid fermentation, in SOLID ORGANIC material per ton, add bacterial strain SQR9 fermented liquid 40 ~ 60L, turning every day 1 ~ 2 time in solid fermentation process, make solid fermentation temperature be no more than 60 DEG C, ferment and finish after 6-7 days, SQR9 content reaches 2 × 10 8more than CFU/g fertilizer dry weight, under the last condition that is no more than 60 DEG C in temperature, the water content of microbial organic fertilizer is evaporated to below 30%, pulverizes, cross 20 mesh sieves; This fertilizer quality of organic matter is than content>=45%, and total nitrogen content is 2-3%;
4) determine the amount of the ammonium sulfate adding according to the concrete nutrient of above-mentioned biological organic fertilizer, ammonium sulfate pulverize after with sieve after biological organic fertilizer raw material mix it is mixed after total nutrient be 10% to obtain mixed fertilizer;
5) utilize granulating disc will obtain mixed fertilizer granulation, after granulating, under temperature is no more than the condition of 50 DEG C, the water content of microbial organic fertilizer be dried to below 20%, " unit entirely " composite microbiological fertilizer.
8. the application of " unit entirely " composite microbiological fertilizer aspect promotion crop growth and volume increase described in any one in claim 1～4.
9. application according to claim 8, is characterized in that the described application of " unit entirely " composite microbiological fertilizer aspect promotion eggplant or tomato growth and volume increase.
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CN105462898A (en) * 2015-12-24 2016-04-06 南京农业大学 Bacillus aquimaris L-60 capable of effectively prompting growth of crops and application thereof
CN106478202A (en) * 2015-08-10 2017-03-08 上海绿乐生物科技有限公司 A kind of high-performance bio foliage fertilizer and its preparation and using method
CN106754449A (en) * 2015-11-19 2017-05-31 王鸣远 A kind of utilization cow dung or horsehit prepare technology and its application of liquid bio-bacterial manure
CN106986729A (en) * 2017-05-04 2017-07-28 浙江德清东鑫复混肥科技有限公司 A kind of special mixed fertilizer of Chinese cabbage
CN107686406A (en) * 2017-09-20 2018-02-13 南京农业大学 A kind of special trichoderma of garlic first bio-organic fertilizer and its preparation method and application entirely
CN108774088A (en) * 2018-07-10 2018-11-09 云南省微生物发酵工程研究中心有限公司 A kind of organic-inorganic complex fertilizer and preparation method thereof with prophyiaxis and promoting growth function
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CN102898197A (en) * 2012-11-01 2013-01-30 南京农业大学 Technology for producing growth promoting bioorganic fertilizer by using algae mud as additive and product
CN102924141A (en) * 2012-11-01 2013-02-13 南京农业大学 Process for utilizing three abandoned proteins and thoroughly-decomposed cow dung to produce growth-promoting bio-organic fertilizer and product thereof

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CN102898197A (en) * 2012-11-01 2013-01-30 南京农业大学 Technology for producing growth promoting bioorganic fertilizer by using algae mud as additive and product
CN102924141A (en) * 2012-11-01 2013-02-13 南京农业大学 Process for utilizing three abandoned proteins and thoroughly-decomposed cow dung to produce growth-promoting bio-organic fertilizer and product thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105036918A (en) * 2015-07-20 2015-11-11 湖南泰谷生物科技股份有限公司 Multielement biologic organic fertilizer and preparing method thereof
CN106478202A (en) * 2015-08-10 2017-03-08 上海绿乐生物科技有限公司 A kind of high-performance bio foliage fertilizer and its preparation and using method
CN106754449A (en) * 2015-11-19 2017-05-31 王鸣远 A kind of utilization cow dung or horsehit prepare technology and its application of liquid bio-bacterial manure
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CN106986729A (en) * 2017-05-04 2017-07-28 浙江德清东鑫复混肥科技有限公司 A kind of special mixed fertilizer of Chinese cabbage
CN107686406A (en) * 2017-09-20 2018-02-13 南京农业大学 A kind of special trichoderma of garlic first bio-organic fertilizer and its preparation method and application entirely
CN108774088A (en) * 2018-07-10 2018-11-09 云南省微生物发酵工程研究中心有限公司 A kind of organic-inorganic complex fertilizer and preparation method thereof with prophyiaxis and promoting growth function
CN113292386A (en) * 2020-02-24 2021-08-24 石小和 Complete-element biological organic fertilizer and preparation method thereof

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