CN105481486A - Method for producing trichoderma bio-organic fertilizer from straw and filtrated mud and obtained product - Google Patents
Method for producing trichoderma bio-organic fertilizer from straw and filtrated mud and obtained product Download PDFInfo
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- CN105481486A CN105481486A CN201610003589.XA CN201610003589A CN105481486A CN 105481486 A CN105481486 A CN 105481486A CN 201610003589 A CN201610003589 A CN 201610003589A CN 105481486 A CN105481486 A CN 105481486A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a method for producing trichoderma bio-organic fertilizer from straw and filtrated mud and an obtained product. Ingredients comprise straw of crops (such as rice, corn and the like), filtrated mud, water (common tap water) and common organic fertilizer. The technological process comprises the steps that the straw and the filtrated mud are mixed according to the mass ratio of 3:2-4:1, the water is added based the water content of 60-65%, sterilization is carried out at 115 DEG C for 30 min, 5-10% inoculation is carried out, shallow tray fermentation is carried out, the fermentation depth is 1-3 cm, the fermentation temperature is 22-28 DEG C, fertilization lasts for 7 days, and after fertilization, fermentation solid and the common organic fertilizer are mixed according to the mass ratio of 1:8-1:10 to form the bio-organic fertilizer. The trichoderma spore number of per g of produced fertilizer is larger than 2*10<7>, the total nitrogen, phosphorus and potassium nutrient content is larger than or equal to 5%, the organic matter content is larger than or equal to 45%, and the growth promotion effect is good.
Description
Technical field
The present invention, in agricultural intensive production technology field, relates to and utilizes stalk and filter mud to produce the method for trichoderma as biological fertilizer and the product of acquisition.
Background technology
Agricultural is the basic industry of China, always in occupation of important position in national economy.Three during the last ten years China's grain-production achieve significant growth, wherein a large amount of uses of chemical fertilizer support the important factor of increases in grain production, and China consumes the chemical fertilizer in the world more than 30% with the arable land accounting for the world 8%.While increase grain yield, long-term excessive use chemical fertilizer also brings degradation problem (GuoJH under environmental pollution, soil compaction, Soil degradation, ecological degeneration and quality of agricultural product, LiuXJ, ZhangY, etal.SignificantacidificationinmajorChinesecroplands [J] .Science, 2010,327:1008-1010).If this situation can not be changed, be unfavorable for the coordinated development of agricultural resource and environment on the one hand, the quality causing these basic capital goods in the agricultural sector of ploughing on the other hand reduces, and is unfavorable for the Sustainable development of agricultural.Microbial fertilizer receives publicity because of its environmental friendliness, resources conservation, green safety.Microbial fertilizer is also known as bio-feritlizer, bacterial manure or Inoculant, a class with microbial life activity and product thereof cause farm crop obtain specific fertilizer effect living microorganisms goods (Ge Cheng. microbial fertilizer production application basis [M]. Beijing: Chinese agriculture Science Press, 2000,1-28.).Microbial fertilizer is at culture fertility, improve chemical fertilizer utilization ratio, suppress farm crop to nitric nitrogen, heavy metal, the absorption of agricultural chemicals, purification and rehabilitating soil, reduce corps diseases to occur, promote that agricultural crop straw becomes thoroughly decomposed utilization, protect the fields environment and improve crop products quality and food safety aspect and showed irreplaceable effect, the key (Zhang Ruifu of agriculture production Sustainable development synchronous with ecotope, Yan Chunrong, Zhang Nan, Deng. microbial fertilizer research and the application prospect [J] in farmland quality promotes thereof, Chinese agriculture science and technology Leader, 2013, 15 (5): 8-16).
Stalk " with then sharp, abandoned, is done harm to ".Society, the exploitation of stalk resource, both the material Efficient Conversion in agricultural production system and energy efficient circulation had been related to, become the important realization means of circular agriculture, also the soil fertility in whole agroecosystem is related to, soil conservation, the sustainable developments such as environmental safety and renewable resources efficiency utilization, relate to again the warm and clean environment of household in life of farmers system, progressively become the inevitable requirement (Bi Yuyun of agriculture and countryside Sustainable Socioeconomic Development, stalk resource Evaluation and utilization research [D]. Chinese Academy of Agricultural Sciences .2010.06.).
In sugar manufacturing process, the non-sugar organic substances such as the protein contained by mixing juice, pectin substance, organic acid are unfavorable to cane sugar boiling and crystal, therefore, must carry out clarifying treatment to mixing juice.Namely the materials such as calcium hydroxide, sulfurous gas, phosphoric acid are added to mixing juice, the non-sugar organism such as protein, pectin substance, organic acid are precipitated, throw out is by pressure filter or vacuum suction filter or centrifugal separation equipment and the filter residue obtained, be called filter mud, it is one of large byproduct of cane sugar manufacture.Filter mud is the mixture of sugarcane epithelium, bagasse, juice,liming agent, earth and sugar etc., rich in proteins, fat, robust fibre, nitrogen-free extract, ash content (Guo Weilie, Guo Qinghua. with cane sugar factory fermentation waste strengthening stromatin [J]. pesticide herd product is developed, and 1996 (5): 29-31).But, the biological utilisation degree of cane sugar factory to filter mud is in lower level, is used as fertilizer on a small quantity, major part is discarded (protect state abundant. Sugar Industry resource comprehensive utilization technology economic policy investigation [J]. Sugar Industry, 1993, (1): 59-64).
Trichoderma is a kind of Antagonism fungi with important utility value, to various plants pathogenic bacteria, there is antagonistic action, be defined as a kind of important bio-control factors (CookRJ, BakerKF.Thenatureandpracticeofbiologicalcontrolofplantpa thogens [J] .AmericanPhytopathologicalSociety, 1983:318).In the nearly more than ten years, a large amount of achievement is achieved to the research of Trichoderma in control of plant disease both at home and abroad, filter out the correlative study of some high efficient strains for Biocontrol Effect, Biocontrol Mechanism and preparation processing, in controlling plant diseases, play more and more important effect.How obtaining a large amount of tunnings such as conidium, mycelium or chlamydospore etc. is one of the processing and production gordian technique of Trichoderma preparation.And solid fermentation is one of best method of the acquisition fungal spore of generally acknowledging at present, not only output is high for it, and quality is good.In solid fermentation, because thalline grows under the condition that humidity is lower, thus the spore usually obtained has more resistance to drought condition, and in a dry environment more stable (bow like monarch, Sun Cuixia, Qiu Lina, waits .Bt biological pesticide [M]. Beijing: Chemical Industry Press, 2006).
Summary of the invention
The object of the invention is to for the practical problems in production practice and demand, develop the composition and engineering utilizing stalk and filter mud solid fermentation development trichoderma as biological fertilizer, reduce the production cost of biological organic fertilizer, the trichoderma as biological fertilizer that production growth-promoting is effective, resistance against diseases is strong.
Object of the present invention realizes by following technical scheme:
Utilize the method for the raw trichoderma as biological fertilizer of stalk filter mud, comprise the following steps:
1) reesei spores liquid is prepared, spore concentration 1 × 10
7 ~9 × 10
7individual/g;
2) above-mentioned reesei spores liquid is inoculated into molasses nutrient solution, carry out liquid fermenting production, the condition of fermentative production is: leavening temperature 28 DEG C, and stirring velocity is 170 ~ 180 revs/min, fermentation time is 48 ~ 60h, makes biomass dry weight in tunning be 6.0 ~ 7.0g/L; Molasses nutrient solution compound method used is, to prepare 1L nutrient solution: molasses 20g, yeast powder 5g, ferrous sulfate 0.1g, bitter salt 1.25g, potassium primary phosphate 4.0g, ammonium sulfate 5.45g, be settled to 1000ml, 115 DEG C of sterilizing 30min;
3) by the stalk of mass ratio 1.5 ~ 4:1, filter mud mixing, add water by 60% ~ 65% water content, 115 DEG C of sterilizing 30min;
4) accessed by mould for wood fermented liquid in the stalk of above-mentioned sterilizing, filtered mud mixture, stir, pave in tray, 1 ~ 3cm, with eight layers of gauze sealing, carry out solid fermentation, inoculum size is 10%, solid fermentation temperature 22 ~ 28 DEG C, ferments and terminates after 7 days; In tunning, reesei spores content reaches 4 × 10
8individual/more than g, water content 30% ~ 40%; By solid fermentation thing and common fertilizer in mass ratio 1:8 ~ 1:10 mix and obtain described trichoderma as biological fertilizer; Described trichoderma harziarum biological organic fertilizer Sino-Kazakhstan thatch reesei spores number is greater than 2 × 10
7individual/g, total npk nutrient is>=5%, quality of organic matter is than content>=45%.
The mould bacterial strain being not limited to particular source of wood described in the present invention, that reported at present or commercially available or to phytopathogen, there is antagonistic action and obtain and be woodenly mouldly all applicable to the present invention from row filter.
Described reesei spores liquid is prepared by the following method: be inoculated into mould for wood on PDA flat board, treats that it covers with whole flat board, is scraped by mycelia with blade, and filter with sterile gauze, with aseptic water washing, filter mycelia, make spore liquid, spore concentration reaches 4 × 10
7individual/g, 4 DEG C of Refrigerator stores, inoculate for subsequent use.
Step 2) middle liquid fermenting time preferably 48 ~ 50h, biomass dry weight preferably 6.0 ~ 6.5g/L in tunning.
Step 3) in stalk, filter mud preferably mix according to mass ratio 3 ~ 4:1.
Step 4) in solid fermentation thing and common fertilizer in mass ratio 1:9 mix and obtain described trichoderma as biological fertilizer.
The trichoderma as biological fertilizer prepared according to the method described in the present invention.
Trichoderma as biological fertilizer of the present invention is promoting the application in field crop growth.
Beneficial effect the invention provides a kind of composition and engineering utilizing stalk and the mould development trichoderma as biological fertilizer of filter mud solid fermentation wood.
The trichoderma as biological fertilizer cost using this invention to produce is lower than traditional biological fertilizer, easy to use, growth-promoting and the good advantage of disease resisting effect, is applicable to field big area and uses.The present invention is for preserving the ecological environment, and the protection people's is healthy, and the added value improving agricultural-food has great importance.
Product tool compared with product in the market has the following advantages:
1) the present invention utilizes stalk and the mould acquisition high density reesei spores fermented product of filter mud solid fermentation wood, mixes with common fertilizer, have developed trichoderma as biological fertilizer.This formula and technique can increase considerably the quantity of reesei spores in the mould biological organic fertilizer of this Novel wooden, and while guarantee effect of fertilizer, reduce the production cost of trichoderma as biological fertilizer, the product of production has the extremely strong market competitiveness.
2) in fertilizer, the content of reesei spores reaches 2 × 10
7individual/more than g, meets national standard.This fertilizer long preservative period, growth-promoting is effective.
3) test-results shows, in base fertilizer, use this product, significantly can promote plant growth.
Accompanying drawing explanation
The different initial water content of Fig. 1 is on the impact of solid fermentation reesei spores number
The different stalk of Fig. 2 and filter mud mass ratio are on the impact of solid fermentation reesei spores number
Fig. 3 different vaccination amount is on the impact of solid fermentation reesei spores number
Fig. 4 different fermentations temperature is on the impact of solid fermentation reesei spores number
Fig. 5 different fermentations cycle is on the impact of solid fermentation reesei spores number
The different initial pH of Fig. 6 is on the impact of solid fermentation reesei spores number
Whether Fig. 7 stirs the impact on solid fermentation reesei spores number
Fig. 8 different fermentations degree of depth is on the impact of solid fermentation reesei spores number
Fig. 9 trichoderma as biological fertilizer growth-promoting design sketch (transplanting latter 30 days)
Figure 10 trichoderma as biological fertilizer growth-promoting design sketch (transplanting latter 60 days)
Biomaterial preservation information
SQR-T037, Classification And Nomenclature is trichoderma harziarum Trichodermaharzianum, be preserved in China Microbiological preservation management committee's common micro-organisms center, preservation address is Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preservation date is on September 22nd, 2009, and deposit number is CGMCCNO.3308.
Embodiment
Embodiment 1
1, the acquisition of stalk
Purchase in peasant household's hand, be broken into powder shape.
2, the acquisition of filter mud
Sugarcane squeezes the purchase of sugared field, crosses 100 mesh sieves
3, the production of time Trichoderma microbial inoculum is breathed out
The culture presevation number of trichoderma harziarum SQR-T037 is CGMCCNO.3308, only explain concrete technical scheme of the present invention and effect for trichoderma harziarum SQR-T037 herein, to phytopathogen, there is antagonistic action and obtain and be woodenly mouldly all applicable to the present invention of other sources.
Be inoculated into mould for wood on PDA flat board, treat that it covers with whole flat board, with blade, mycelia is scraped, filter with sterile gauze, with aseptic water washing, filter mycelia, make spore liquid, spore concentration general 4 × 10
7individual/g, 4 DEG C of Refrigerator stores, inoculate for subsequent use.Above-mentioned reesei spores liquid is inoculated into molasses nutrient solution, carry out liquid fermenting production, the condition of fermentative production is: leavening temperature 28 DEG C, and stirring velocity is 170 ~ 180 revs/min, fermentation time is 48 ~ 60h, makes biomass dry weight in fermented liquid be 6.0 ~ 7.0g/L; Molasses nutrient solution compound method used is, to prepare 1L nutrient solution: molasses 20g, yeast powder 5g, ferrous sulfate 0.1g, bitter salt 1.25g, potassium primary phosphate 4.0g, ammonium sulfate 5.45g, be settled to 1000ml, 115 DEG C of sterilizing 30min;
3, solid fermentation makes trichoderma as biological fertilizer
The acquisition of 3.1 solid fermentation formulas and condition
Method of going forward one by one is utilized to obtain the formula of solid fermentation, first with single factor experiment, exploration is carried out to solid fermentation condition and comprise following condition: in the mass ratio of initial water content, stalk and filter mud, whether sterilizing, inoculum size, leavening temperature, fermentation period, initial pH, fermentation, whether need stirring, deep fermentation etc., then larger on wherein several impact factor carries out orthogonal test, final Optimum formulae and the condition obtaining solid fermentation.Formula used is crop material (paddy rice, corn etc.), filter mud, water (ordinary tap water), common fertilizer; Technical process is by stalk and filter mud 4:1 mixing in mass ratio, add water by mixture water content 60wt%, 115 DEG C, 30min sterilizing, 10% access wood is mould, shallow tray fermentation, deep fermentation is 1 ~ 3cm, leavening temperature 22 ~ 28 DEG C, ferment 7 days, after fermentation by solid fermentation thing and common fertilizer in mass ratio 1:9 be mixed into biological organic fertilizer.The fertilizer reesei spores number produced is greater than 2 × 10
7individual/g, total npk nutrient>=5%, organic content>=45%, growth-promoting is effective, and resistance against diseases is strong.
The quantity of the function yeast in each process of rear mensuration of having fermented, result is as Fig. 1 ~ 8, and reesei spores content can reach 4 × 10
8individual/more than g.
3.2 with fertilizer mixture manufacturing finished product trichoderma as biological fertilizer
Fertilizer used is the connection industry refined organic fertilizer produced by Jiangyin Lianye Biotechology Co., Ltd..
By solid fermentation thing and fertilizer in mass ratio 1:9 mix, packaging is dispatched from the factory and is trichoderma as biological fertilizer; The trichoderma as biological fertilizer reesei spores number > 2 × 10 be developed into
7individual/g, total npk nutrient>=5%, organic content>=45%.Above result shows that developed composition and engineering completely can the mould biological organic fertilizer of maximized fermentative production Novel wooden, and this trichoderma as biological fertilizer meets national biological organic fertilizer standard.
4, greenhouse test
Growth-promoting test is carried out in resource and environmental science institute of Agricultural University Of Nanjing greenhouse, supplying to study thing is corn, kind is that field, capital is pale reddish brown glutinous, four are established to contrast a process altogether, be treated to the mould biological organic fertilizer of Novel wooden (BF) of the present invention's development, contrast be respectively do not add any fertilizer (CK), chemical fertilizer process (CF), common fertilizer (OF1), common fertilizer mix with stalk filter mud fertilizer (OF2), (nutrient such as CF, OF1, OF2 and BF, OF2 and BF matrix is identical).Each process 5 basin, every basin fills 5 ㎏ soil, and the addition of fertilizer is 1%.Fertilizer fully mixes with soil, Routine Management.Nursery in seedling pan, treats that it grows to three leaves, chooses uniform seedling replanting in basin, every basin one young plant.Transplanting took pictures as Fig. 9 after 30 days, took pictures as Figure 10 after 60 days, and receive seedling and measure growth index, result is as table one, table two.1) stem is thick: compared with four contrasts, and the BF process of the present invention's development can increase the stem of plant slightly, shows significant difference.2) chlorophyll content: compared with four contrasts, the BF process of the present invention's development can increase the chlorophyll content of plant, shows significant difference.3) plant height: compared with four contrasts, the BF process of the present invention's development can increase the plant height of plant, shows significant difference.4) root is long: compared with four contrasts, and the root that the BF process that the present invention develops can increase plant is long, shows significant difference.5) overground part fresh weight and dry weight: compared with four contrasts, the BF process of the present invention's development can increase plant above ground portion fresh weight and dry weight, shows significant difference.6) underground part fresh weight and dry weight: compared with four contrasts, the BF process of the present invention's development can increase plant above ground portion fresh weight and dry weight, shows significant difference.
Test-results entirety shows, the trichoderma as biological fertilizer BF of the present invention's development significantly can promote the growth of plant.
The impact that table one different treatment is thick on Maize Stem, leaf green content, plant height, root are long
Table two different treatment is to corn field top fresh weight, dry weight; The impact of underground part fresh weight, dry weight
Claims (7)
1. utilize stalk filter mud to produce the method for trichoderma as biological fertilizer, it is characterized in that comprising the following steps:
1) reesei spores liquid is prepared, spore concentration 1 × 10
7~ 9 × 10
7individual/g;
2) above-mentioned reesei spores liquid is inoculated into molasses nutrient solution, carry out liquid fermenting production, the condition of fermentative production is: leavening temperature 28 DEG C, and stirring velocity is 170 ~ 180 revs/min, fermentation time is 48 ~ 60h, makes biomass dry weight in tunning be 6.0 ~ 7.0g/L; Molasses nutrient solution compound method used is, to prepare 1L nutrient solution: molasses 20g, yeast powder 5g, ferrous sulfate 0.1g, bitter salt 1.25g, potassium primary phosphate 4.0g, ammonium sulfate 5.45g, be settled to 1000ml, 115 DEG C of sterilizing 30min;
3) stalk of mass ratio 1.5 ~ 4:1 is mixed with filter mud, add water by mixture 60% ~ 65% water content, 115 ~ 120 DEG C of sterilizing 20 ~ 30min;
4) accessed by mould for wood fermented liquid in the stalk of above-mentioned sterilizing, filtered mud mixture, stir, carry out solid fermentation, inoculum size is 5% ~ 10%, solid fermentation temperature 22 ~ 28 DEG C, ferments and terminates after 7 days; In tunning, reesei spores content reaches 4 × 10
8individual/more than g, water content 30% ~ 40%; Solid fermentation thing in mass ratio with common fertilizer is mixed and obtains described trichoderma as biological fertilizer in 1:8 ~ 10; In described trichoderma as biological fertilizer, reesei spores number is greater than 2 × 10
7individual/g fertilizer dry weight, total npk nutrient is>=5%, and quality of organic matter is than content>=45%.
2. the method utilizing stalk filter mud to produce trichoderma as biological fertilizer according to claim 1, it is characterized in that described reesei spores liquid is prepared by the following method: be inoculated on PDA flat board by mould for wood, treat that it covers with whole flat board, with blade, mycelia is scraped, filter with sterile gauze, with aseptic water washing, filter mycelia, make spore liquid, spore concentration reaches 1 × 10
7~ 9 × 10
7individual/g, 4 DEG C of Refrigerator stores, inoculate for subsequent use.
3. the method utilizing stalk filter mud to produce trichoderma as biological fertilizer according to claim 1, is characterized in that step 2) in the liquid fermenting time be 48 ~ 50h, in tunning, biomass dry weight is 6.0 ~ 6.5g/L.
4. the method utilizing stalk filter mud to produce trichoderma as biological fertilizer according to claim 1, is characterized in that step 3) in stalk, filter mud mix according to mass ratio 3 ~ 4:1.
5. the method utilizing stalk filter mud to produce trichoderma as biological fertilizer according to claim 1, is characterized in that step 4) in solid fermentation thing and common fertilizer in mass ratio 1:9 mix and obtain described trichoderma as biological fertilizer.
6. according to trichoderma as biological fertilizer prepared by the method according to any one of Claims 1 to 5.
7. trichoderma as biological fertilizer according to claim 6 is promoting the application in field planting species growth.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085871A (en) * | 2016-06-18 | 2016-11-09 | 南京农业大学 | A kind of be raw material production Trichoderma spp. solid spawn with amino acid hydrolyticsolution and rice straw method |
| CN106348888A (en) * | 2016-08-31 | 2017-01-25 | 山东省科学院生态研究所 | Method for producing trichoderma bio-organic fertilizer by using green Chinese onion leaf waste |
| CN108129234A (en) * | 2017-12-28 | 2018-06-08 | 南京农业大学 | A kind of special complete first trichoderma as biological organic fertilizer for restoring old apple tree productivity and its preparation method and application |
| CN110156511A (en) * | 2019-04-12 | 2019-08-23 | 浙江省农业科学院 | A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material |
| CN113372177A (en) * | 2021-06-15 | 2021-09-10 | 华强化工集团股份有限公司 | Special biological organic-inorganic compound fertilizer for garlic and preparation method thereof |
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| CN103694007A (en) * | 2013-11-18 | 2014-04-02 | 广西金穗生物科技有限责任公司 | Double fermentation process for bio-organic fertilizer production |
| CN104496570A (en) * | 2014-12-24 | 2015-04-08 | 广西力源宝科技有限公司 | Method for preparing organic fertilizer from sugar mill mud |
| CN104609995A (en) * | 2015-01-22 | 2015-05-13 | 河海大学 | Plant growth promoting bio-organic fertilizer for saline-alkali land |
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Patent Citations (4)
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| CN101696390A (en) * | 2009-10-29 | 2010-04-21 | 南京农业大学 | Biological preventing and controlling strain of continuous cropping cucumber and watermelon blight and microbe organic fertilizer thereof |
| CN103694007A (en) * | 2013-11-18 | 2014-04-02 | 广西金穗生物科技有限责任公司 | Double fermentation process for bio-organic fertilizer production |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085871A (en) * | 2016-06-18 | 2016-11-09 | 南京农业大学 | A kind of be raw material production Trichoderma spp. solid spawn with amino acid hydrolyticsolution and rice straw method |
| CN106348888A (en) * | 2016-08-31 | 2017-01-25 | 山东省科学院生态研究所 | Method for producing trichoderma bio-organic fertilizer by using green Chinese onion leaf waste |
| CN106348888B (en) * | 2016-08-31 | 2019-09-10 | 山东省科学院生态研究所 | A method of trichoderma as biological organic fertilizer is produced using shallot Folium Allii fistulosi waste |
| CN108129234A (en) * | 2017-12-28 | 2018-06-08 | 南京农业大学 | A kind of special complete first trichoderma as biological organic fertilizer for restoring old apple tree productivity and its preparation method and application |
| CN110156511A (en) * | 2019-04-12 | 2019-08-23 | 浙江省农业科学院 | A kind of biological organic fertilizer and preparation method thereof based on mineral humus and vegetables residuum trichoderma fermentation material |
| CN113372177A (en) * | 2021-06-15 | 2021-09-10 | 华强化工集团股份有限公司 | Special biological organic-inorganic compound fertilizer for garlic and preparation method thereof |
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|---|---|
| CN105481486B (en) | 2019-01-18 |
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