CN103960171B - Bohadschia argus Jaeger fingerlings artificial culturing method - Google Patents
Bohadschia argus Jaeger fingerlings artificial culturing method Download PDFInfo
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- CN103960171B CN103960171B CN201310032664.1A CN201310032664A CN103960171B CN 103960171 B CN103960171 B CN 103960171B CN 201310032664 A CN201310032664 A CN 201310032664A CN 103960171 B CN103960171 B CN 103960171B
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- argus jaeger
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- 241000961078 Bohadschia argus Species 0.000 title claims abstract description 25
- 241000529895 Stercorarius Species 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000012258 culturing Methods 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 241000208340 Araliaceae Species 0.000 claims abstract description 20
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 20
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 20
- 235000008434 ginseng Nutrition 0.000 claims abstract description 20
- 230000001418 larval effect Effects 0.000 claims abstract description 13
- 230000016087 ovulation Effects 0.000 claims abstract description 7
- 230000007613 environmental effect Effects 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims description 17
- 241000593522 Sargassum thunbergii Species 0.000 claims description 8
- 230000035800 maturation Effects 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 6
- 230000002710 gonadal effect Effects 0.000 claims description 6
- 241000227752 Chaetoceros Species 0.000 claims description 5
- 241000195493 Cryptophyta Species 0.000 claims description 5
- 241000223252 Rhodotorula Species 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 240000002900 Arthrospira platensis Species 0.000 claims description 3
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 3
- 241000237509 Patinopecten sp. Species 0.000 claims description 3
- 241000195474 Sargassum Species 0.000 claims description 3
- 210000001647 gastrula Anatomy 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 230000000384 rearing effect Effects 0.000 claims description 3
- 235000020637 scallop Nutrition 0.000 claims description 3
- 229940082787 spirulina Drugs 0.000 claims description 3
- 230000004936 stimulating effect Effects 0.000 claims description 3
- 230000002354 daily effect Effects 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 description 14
- 238000009395 breeding Methods 0.000 description 3
- 241000965254 Apostichopus japonicus Species 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241000221377 Auricularia Species 0.000 description 1
- 208000031872 Body Remains Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000220010 Rhode Species 0.000 description 1
- 229920001617 Vinyon Polymers 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000023011 digestive tract development Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of Bohadschia argus Jaeger fingerlings artificial culturing method.The present invention sets specification, environmental condition and the bait composition that parent participation is cultivated, and grows to after the specific time at it, dries in the shade and mode that strong current stimulate carries out artificial induced spawning with lucifuge.The ovulation of parent participation ejaculation produces after fertilized egg, controls the life condition of fertilized egg, through fertilized egg preferably and counting, enter the larval culture stage, the young feed of the hand fit that throws something and feeds and carry out water quality environment management.After the young enters large ear larval stage, drop into adherance, and start to adjust bait formation, carry out the water quality management of post-larval.Until paedomorphosis is young ginseng.Adopt the artificial culturing method of Bohadschia argus Jaeger seed that the present invention relates to, making Bohadschia argus Jaeger by only can the economic kind of artificial culture by manually adopting that the wild economic kind of catching changes into, can start to propagate artificially.
Description
Technical field
The present invention relates to a kind of Bohadschia argus Jaeger fingerlings artificial culturing method.
Background technology
Bohadschia argus Jaeger is by the economic kind of Tropical Ocean of the present invention's artificial culture newly developed, does not also have the technical data of artificial seed rearing at present.
Artificial seed's breeding method of imitative stichopus japonicus is ripe, propagate the output that can produce 10,000,000,000 yuan artificially, become the Important Economic kind of mariculture, the basis that imitative stichopus japonicus is propagated artificially is exactly that artificial seed cultivates the method establishing maturation, although in the cultivation of seed ginseng, artificial insemination, there are roughly the same step and link in the cultivation aspect of larval culture and young ginseng, but artificial seed's breeding method of Bohadschia argus Jaeger is based on the Developmental Biology of Bohadschia argus Jaeger itself, environmental ecology and each stage bait compositing characteristic, for the technical method of concrete artificial seed's cultivation that the economic kind of this new Tropical Ocean of Bohadschia argus Jaeger is invented.
Summary of the invention
The object of the invention is to provide a kind of Bohadschia argus Jaeger fingerlings artificial culturing method.
For achieving the above object, the present invention adopts technical scheme to be:
A kind of Bohadschia argus Jaeger fingerlings artificial culturing method, by Bohadschia argus Jaeger parent participation through artificial culture to gonadal maturation; Hastened parturition by strong stimulation by running water under the water temperature of 30-31 DEG C, induction parent participation ovulation or ejaculation, produce fertilized egg, incubating oosperm is cultivated and is generated the young, when paedomorphosis is to doliolaria, adherance is thrown in, with salt algae, Chaetoceros, ocean rhodotorula and food calling yeast for bait carries out seed rearing in cultivation pond.
Cultivate to gonadal maturation with specification 350 grams to 450 grams Bohadschia argus Jaeger parent participations in seed ginseng cultivation pond; In cultivating process, daily ration of feeding is by the 4-6% of parent participation TBW; The environmental condition of cultivating is that water temperature remains on 26 DEG C--31 DEG C, DO value remains on more than 5ppm.
Be equipped with Sha Heni bottom described cultivation pond, wherein husky and mud is 4:6 in the ratio of quality; Bait is formed: the 200 order sargassum powder of 8% or sargassum thunbergii powder, the seabed mud scum of 90%, the scallop edge powder of 1% and 1% 200 order conch meals compositions.
Drying in the shade cultivating to pull out to the parent participation of gonadal maturation 40-60 minutes, after stimulating 5-10 minute with the current of 3 meter per second flow velocitys, seed ginseng being put into water temperature and inducing parent participation ovulation or ejaculation in the cultivation pond of 30-31 DEG C.
When incubating oosperm is to primitive gut later stage or ear initial stage, the young is carried out cloth pond by 0.5/ml.
To throw something and feed the bait be made up of salt algae 2ppm, Chaetoceros 4ppm, ocean rhodotorula 2ppm and food calling yeast 1ppm in incubating oosperm to the doliolaria stage; And in the whole larval culture stage, cultivate pool inner water body and change, replacing every day amount is cultivate the 1/3-1/2 of pond volume.
Adherance is thrown in when paedomorphosis is doliolaria in the water of described cultivation pond, and make it to be covered with whole cultivation pond, after the young is attached to and adherance grows 11-12 days, using ooze 5ppm, sargassum thunbergii powder 5ppm, spirulina powder 0.3ppm as bait, until obtain young ginseng.
The advantage that the present invention has:
Specification, environmental condition and the bait composition that the present invention is cultivated by setting parent participation, and growing to after the specific time at it, dries in the shade with lucifuge and mode that strong current stimulate carries out artificial induced spawning.After parent participation ovulation, control the life condition of fertilized egg, through fertilized egg preferably and counting, enter the larval culture stage, the young feed of the hand fit that throws something and feeds and carry out water quality environment management.After the young enters large ear larval stage, drop into adherance, and start to adjust bait formation, carry out the water quality management of post-larval, until paedomorphosis is young ginseng.
Adopt the artificial culturing method of Bohadschia argus Jaeger seed that the present invention relates to, making Bohadschia argus Jaeger by only can the economic kind of artificial culture by manually adopting that the wild economic kind of catching changes into, can start to propagate artificially.
Embodiment
Embodiment 1
Bohadschia argus Jaeger artificial seed breed:
1) selection of parent participation and cultivation:
The specification that parent participation is cultivated: 350 grams to 450 grams
The temperature that parent participation is cultivated: 26 DEG C--31 DEG C
The environment that parent participation is cultivated:
Arrange husky mud bottom environment in the cement pit of 1. 3.5 meters × 6.5 meters × 1.8 meters, silt ratio is 4:6;
2. pool inner water is 1.3 meters-1.5 meters deeply, miniflow water, and streamflow makes water in pond be able to whole replacing in 3 days;
3. use rhodes blower inflation, make DO value in breeding water body remain on more than 5ppm;
4. change pond once every 5 days, at the bottom of the pool wall emptied, pond, brush the dirt of all kinds of deposition with brush.
The bait that parent participation is cultivated:
1. bait composition (percentage by weight): 8%200 order sargassum powder, 90% seabed mud scum, 1% scallop edge powder and 1%150 order conch meals;
2. bait feeding: every day throws something and feeds by the 4-6% of parent participation gross weight in pond.
2) artificial induced spawning of parent participation:
1. parent participation is selected: choose and be above-mentionedly cultured to parent participation body weight more than 350 grams, slightly bossed individuality is felt at belly sexual gland position;
2. to hasten parturition mode: pulled out from pond by seed ginseng and dry in the shade 40-60 minutes, after stimulating 5-10 minute with the current of 3 meter per seconds, seed ginseng is put into spawning pond, and the water temperature in spawning pond remains on 30 ° DEG C 31 DEG C.
3) ovulation and fertilization:
Under normal circumstances after male ginseng ejaculation soon, female ginseng just starts to ovulate; As hero joins a large amount of ejaculation, should the hero ginseng just in ejaculation be pulled out in time, put into storage pond, holding pond, ovulation continues 2-4 hour, and cultivate the faint inflation in pond, aeration quantity can make to be evenly distributed in fertilized egg pool water within.
4) fertilized egg preferably and counting
1. basis of microscopic observation incubating oosperm situation, and count.Water in hatchery is stirred evenly, with probe tube multidraw in pond, put into the beaker of 1000ml, inwardly blow with 2ml suction pipe, fertilized egg is evenly distributed in cup, gets 2ml water and observe fertilized egg quantity, according to said method repeatedly sample, take the mean and be about density of fertilized egg, be multiplied by with density the quantity that water body is fertilized egg.
2. incubating oosperm can grow primitive gut later stage or auricularia initial stage after 36 hours, select excellent in time.General siphonage, fertilized egg in hatching is siphoned in 260 order dredgers, in time the fertilized egg in net is put into tank, stirred evenly after collecting fertilized egg, cloth pond is carried out by 0.5/ml according to hatching ovum total amount, faint inflation behind cloth pond, aeration quantity can make fertilized egg be evenly distributed in water.
5) larval culture:
1., in larval culture process, whether microscopy is observed larva digestive tract and is grown complete, and bait of should throwing something and feeding in time as complete in its gut development, bait consists of: salt algae 2ppm, Chaetoceros 4ppm, ocean rhodotorula 2ppm, food calling yeast 1ppm.
2. in larval culture process, every day changes water once, and quantity of exchanged water is cultivate pond 1/3rd, is progressively increased to 1/2nd.Changing water method is: put into pond siphon water outlet with 260 order net cages.Siphon is complete the larva clear water that is bonded on net cage is sprinkled under.
6) adherance is thrown in
1. make it partly to float in water with parcel pendant thing in the middle of 30cm × 30cm vinyon cloth, as the adherance of the young, larvae attachment substratum should be covered with nursery pond substantially.
2. to the 8th day, observing young change from larval culture in time, is now large ear larval stage.The change of main detection larva stomach shape and body cavity.When there being doliolaria to occur, start to throw in above-mentioned adherance.
3. timely microscopy larvae development situation is wanted, Individual Size, variable density.Throw in adherance within second day, to start to throw in ooze and sargassum thunbergii powder 4-5ppm, ooze and sargassum thunbergii powder respectively account for 50% by weight.
4. larval culture to fortnight starts to change throw something and feed ooze 5ppm, sargassum thunbergii powder 5ppm, spirulina powder 0.3ppm.After this visually observe, on pool wall and adherance, visible many little projections, are young ginseng, realize the cultivation of seedling kind.
Then can carry out adjusting above-mentioned bait (as adjusted the ratio of sargassum thunbergii powder and ooze gradually) according to the situation of ingesting of ginseng young on adherance and actual needs, more generally start down pond at 20-25 days according to residual bait situation, receive the end with 120 order mesh bags.
Adopt above-described embodiment, every ten female ginsengs of fully-developed and two male ginsengs can produce 1,000 ten thousand fertilized eggs, and the quantity being developed to doliolaria is 3,000,000, and the quantity of growing for young ginseng is 200,000.
Claims (6)
1. a Bohadschia argus Jaeger fingerlings artificial culturing method, is characterized in that: by Bohadschia argus Jaeger parent participation through artificial culture to gonadal maturation; Dry in the shade cultivating to pull out to the parent participation of gonadal maturation 40-60 minutes, after stimulating 5-10 minute with the current of 3 meter per second flow velocitys, parent participation is put into water temperature and induce parent participation ovulation or ejaculation in the cultivation pond of 30-31 DEG C, produce fertilized egg, incubating oosperm is cultivated and is generated the young, when paedomorphosis is to doliolaria, in cultivation pond, throw in adherance, with salt algae, Chaetoceros, ocean rhodotorula and food calling yeast for bait carries out seed rearing.
2. by Bohadschia argus Jaeger fingerlings artificial culturing method according to claim 1, it is characterized in that: cultivate to gonadal maturation with specification 350 grams to 450 grams Bohadschia argus Jaeger parent participations in seed ginseng cultivation pond; In cultivating process, daily ration of feeding is by the 4-6% of parent participation TBW; The environmental condition of cultivating is that water temperature remains on 26 DEG C--31 DEG C, DO value remains on more than 5ppm.
3. by Bohadschia argus Jaeger fingerlings artificial culturing method according to claim 2, it is characterized in that: described seed ginseng is cultivated bottom pond and is equipped with Sha Heni, wherein husky and mud is 4:6 in the ratio of quality; Bait is formed: the 200 order sargassum powder of 8% or sargassum thunbergii powder, the seabed mud scum of 90%, the scallop edge powder of 1% and 1% 200 order conch meals compositions.
4., by Bohadschia argus Jaeger fingerlings artificial culturing method according to claim 1, it is characterized in that: when incubating oosperm is to primitive gut later stage or ear initial stage, the young is carried out cloth pond by 0.5/ml.
5. by Bohadschia argus Jaeger fingerlings artificial culturing method according to claim 1, it is characterized in that: to throw something and feed the bait be made up of salt algae 2ppm, Chaetoceros 4ppm, ocean rhodotorula 2ppm and food calling yeast 1ppm in incubating oosperm to the doliolaria stage; And in the whole larval culture stage, cultivate pool inner water body and change, replacing every day amount is cultivate the 1/3-1/2 of pond volume.
6. by Bohadschia argus Jaeger fingerlings artificial culturing method described in claim 1 or 5, it is characterized in that: when paedomorphosis is doliolaria in the water of described cultivation pond, throw in adherance, and make it to be covered with whole cultivation pond, after the young is attached to and adherance grows 11-12 days, using ooze 5ppm, sargassum thunbergii powder 5ppm, spirulina powder 0.3ppm as bait, until obtain young ginseng.
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| CN106912417B (en) * | 2015-12-25 | 2020-03-10 | 中国科学院海洋研究所 | Industrial breeding method for Munseng sempervirens |
| CN109804953B (en) * | 2019-04-08 | 2021-04-13 | 中国水产科学研究院黄海水产研究所 | Breeding method of artificial fry of eupatorium adenophorum |
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