CN103947609A - Method for observing wax gland orifices of eriosoma lanigerum - Google Patents
Method for observing wax gland orifices of eriosoma lanigerum Download PDFInfo
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Abstract
The invention discloses a method for observing wax gland orifices of eriosoma lanigerum. The method comprises the following steps of collecting specimens, initially treating the eriosoma lanigerum, implementing degreasing treatment, preparing slide specimens, observing specimens, analyzing data and the like; researches show that four rows of wax gland orifices, the total up to 50, develop in each of the body backs of the eriosoma lanigerum at any stage; one wax gland orifice develops in each of segments, except two center rows; one wax gland orifice also develops in each of other body segments, except the second segment and the third segment of the head and the eighth segment of the abdomen, of two side rows; each wax gland orifice is formed by a plurality of wax gland micropores arranged in the shape of a plum blossom; the micropores of the first row and the fourth row are remarkably more than the micropores of the second row and the third row; the wax gland micropores of the abdomen are more than the wax gland micropores of other parts; the wax gland micropores of the fourth to the seventh segments of the abdomen are most. According to the method, as the eriosoma lanigerum is rapidly identified by observing and researching the shapes and the arrangement characteristics of the wax gland orifices of the eriosoma lanigerum at any stage, the checkpoint quarantine and the prevention and treatment about the eriosoma lanigerum are provided with experimental bases; the method has the advantages of high precision and strong operability, thereby being applicable for rapid quarantine in the checkpoint quarantine.
Description
Technical field
The present invention relates to a kind of wooly aphis Morphological Identification method, be specifically related to a kind of wooly aphis wax gland hole observational technique, belong to plant protection field.
Background technology
Wooly aphis (
eriosoma lanigerum(Hausmann)) be under the jurisdiction of Semiptera (Hemiptera), Eriosomatidae (Pemphigidae), woolly aphid belong to (
eriosomaleech), having another name called color aphid, red aphid, woolly aphid, is one of domestic and international quarantine object, is also the important quarantine pest of Malus.If the one-tenth aphid of wooly aphis and aphid plesiomorphism, the one-tenth aphid of the female aphid of aptery orphan is closely oval, russet, side has strumae thing, raw undercoat, and health is by the continuous wadding of adularescent wax thing, winged viviparious female aphid health crineous, head and chest black, the continuous wadding of body surface covering white thing is fewer than aptery viviparous one-tenth aphid.If aphid is totally 4 ages, it is cylindric that health is slightly, and body colour is russet, and beak is elongated, extends back.Feeler 5 saves, and health is by the continuous wadding of adularescent thing, if there are wing aphid health both sides to have black wing bud.
Wooly aphis can the anniversary apple tree on the ground, the parasitic thorn of underground part inhales resin, the digestive ferment that can salivate, stimulates parasitic hyperblastosis to form tumour simultaneously, affects the nutrition transporting of apple tree.And wooly aphis secretes white wax continuous wadding thing and excreta honeydew, wherein the main component of honeydew is amino acid and sugar, is that many pathogenic and saprophytic property fungies are (as fumagine bacterium (the idle bacterium of coal
gloeoaes Pomigena)) ideal culture medium, fumagine bacterium can be caused tree body and blade blackout, and leaf photosynthesis is reduced, and makes fruit surface form fruit russeting, stain, affect the attractive in appearance and sense of taste of fruit, therefore preventing and treating wooly aphis becomes the key factor that affects Apple production.
The continuous wadding of the white wax thing of the wooly aphis body back of the body is by secreting wax gland secretion in body, being excreted to external by the wax gland hole of body wall.There is the wax gland hole of 4 files at the back side of wooly aphis, it has certain arrayed feature and the regularity of distribution, can secrete the continuous wadding of white wax thing, there is important effect for the vital movement of wooly aphis: continuous wadding thing can wrap up the honeydew of thickness, make honeydew avoid polluting, glued by honeydew and tie up and cover, keep normal physiological activity; Reduce the pollution of fungi; Continuous wadding thing can be protected wooly aphis to avoid rain drop erosion and predator, post survivor's injury; Meanwhile, continuous wadding thing can uv reflectance and solar radiation, reduces its injury to wooly aphis, and reduces moisture loss; In addition, continuous wadding thing is wrapped in wooly aphis, and Yi Beiren, animal, the vehicles etc. carry and propagate into Pest-or disease-free area, are conducive to diffusion and spread.
Import into China Weihai from wooly aphis in 1914, since the century, be passed to most of apple producing region of China, serious on the yield and quality impact of apple.And the discriminating Main Basis external morphology method to wooly aphis in the quarantine of the outpost of the tax office, and only according to external morphology method, often false retrieval or undetected, causes wooly aphis further to expand.
Summary of the invention
Technical problem to be solved by this invention is for the deficiency that only relies at present external form characterized wooly aphis, a kind of wooly aphis wax gland hole observational technique is provided, by research wooly aphis wax gland number of perforations, arrayed feature and the regularity of distribution, understand physiology course and the function of the continuous wadding of wooly aphis secretion, precise Identification wooly aphis, control its its spread in china, for correct identification wooly aphis in the quarantine of the outpost of the tax office and prevent that diffusion from spreading foundation is provided; The method has advantages of that accuracy is high, strong operability, is applicable to the rapid quarantine in the quarantine of the outpost of the tax office.
In order to achieve the above object, the present invention is achieved in that
A kind of wooly aphis wax gland hole observational technique, comprises the following steps:
(1) sample collection: the wooly aphis harm phase in 5～June, select to be subject to medicament to affect little apple tree, cut the branch with wooly aphis with scissors, the apple branch of handling with care, prevents that polypide from dropping from branch; With scissors, apple leaf is cut, only left petiole, prevent the rising excessive wooly aphis existence that affects of apple branch; The apple stem cutting of handling well is entered to be equipped with in the 1000mL large beaker of 2/3rds water, beaker mouth is sealed with gauze, be put in shady and cool ventilation place for subsequent use;
(2) wooly aphis is just processed: choose a wooly aphis worm and fall, remove covering the silk floss wadding of wooly aphis worm on falling with tweezers, gently worm is fallen to being swept in culture dish with writing brush, binocular entity anatomical lens lower point length of time, in this process, to gently sweep or picking wooly aphis, prevent limb injury; After wooly aphis is swept and falls in culture dish, be that 70-90% alcohol cleans wooly aphis 3 times repeatedly by volumetric concentration, its wax is with it separated with aphid body with continuous wadding; Finally by each length of time wooly aphis put into respectively dactylethrae, be that 70-80% soaking in alcohol 24 hours is above stand-by by volumetric concentration;
(3) wooly aphis ungrease treatment: wooly aphis not of the same age in step 2 is taken out from dactylethrae, prick an aperture with insect needle at its belly, be then put into and fill the standing 24-48h of immersion in the dactylethrae that volumetric concentration is 70-80% alcohol; Then wooly aphis is taken out from alcoholic solution, and in culture dish, rinse 2-3 time with distilled water, the wooly aphis in the different length of times is soaked in respectively in the sodium hydroxide solution that mass concentration is 8-15%, and the insulating box that is placed in 37 DEG C is placed 36-48h, to polypide blushing transparent, from sodium hydroxide solution, take out, with distilled water cleaning 3 times, and again prick another aperture with insect needle at its belly, then wooly aphis is equipped with in the dactylethrae that volumetric concentration is 70-80% alcoholic solution by transferring to the length of time, again leave standstill transparent to polypide till;
(4) making of wooly aphis slide sample: taking out degreasing from dactylethrae is better the transparent wooly aphis of polypide, is put on concave slide, with distilled water flushing 3-5 time by clean alcoholic solution rinsing; Then put it on concavity slide, water is exhausted with blotting paper, coloring agent dyeing is after 5-15 minute, coloring agent rinsed well with distilled water; With suction pipe draw a wooly aphis of having dyeed, be placed on slide, and added a distilled water, then slide is put under binocular entity anatomical lens, with insect needle to the whole appearance of polypide; Along the whole appearance direction of polypide, water is blotted with blotting paper, then make compressing tablet with Huo Shi mounting medium after polypide is left standstill to 5min, while dripping Huo Shi mounting medium and cover glass, will note preventing Bubble formation; The slide making, as for alcolhol burner flame envelope place, heating 30-50 second, is dried to Huo Shi mounting medium and solidified and the bubble in slide is driven away; Slide is lain against in Riker mount, treat that mounting medium inserts in Riker mount after having done completely again;
(5) sample is observed and data processing: make better if select, wax gland hole more clearly 1-4 aphid in age, become each 5 pieces of aphid slide sample, under binocular stereomicroscope, observe number and the distributional pattern thereof of its wax gland hole and aperture, record data are also analyzed; Experimental data is processed with Excel, and utilizes the multiple ratio of SPSS17.0 statistics software each group of experimental data to be carried out to multiple ratio.
Preferably, in step 2, the alcohol concentration that described cleaning wooly aphis is used is 75%; The concentration of described immersion alcohol is 75%, and soak time is 24-48h.
Preferably, in step 2, described writing brush is small size, fur writing brush; In step 3, described insect needle is No. zero insect needle.
Preferably, in step 3, described alcohol concentration is 75%, and described naoh concentration is 10%.
Preferably, in step 4, described coloring agent is BG, and mass concentration is 0.5%, and dyeing time is 10 minutes.
The invention has the beneficial effects as follows: the invention provides a kind of wooly aphis wax gland hole observational technique, accuracy is high, strong operability, be applicable to the rapid quarantine in outpost of the tax office quarantine, can precise Identification wooly aphis, control in time its its spread in china.
Brief description of the drawings
Fig. 1: if four row wax gland hole arrayed feature figure on wooly aphis aphid body wall in 2 age.
embodiment
Below in conjunction with embodiment, wooly aphis wax gland of the present invention hole observational technique is further described.
Embodiment 1:
1, for examination worm source:
Pick up from the wooly aphis in the apple orchard of the regional extensive management in Laiyang Shandong Province area and Chengyang.
2, for test instrument and material:
Binocular stereomicroscope, binocular entity anatomical lens, insulating box, label paper, tweezers, beaker, graduated cylinder, glass bar, slide, concave slide, cover glass, filter paper, blotting paper, Huo Shi mounting medium, alcoholic solution, BG coloring agent, sodium hydrate solid, distilled water etc.
3, test method:
(1) collection of specimens:
The wooly aphis harm phase in 5～June, in apple orchard, select to be subject to medicament to affect little fruit tree, carefully the branch with wooly aphis is cut with scissors, take to indoor, in this process, the apple branch of handling with care and gathering, prevents that polypide from falling down from branch; With scissors, apple leaf is cut, only left petiole, the apple stem cutting of handling well is entered to be equipped with in the 1000mL beaker of 2/3rds water, beaker mouth is sealed with gauze, be put in shady and cool ventilation place for subsequent use.
(2) wooly aphis is just processed:
Choose a wooly aphis worm and fall, remove covering the silk floss wadding of wooly aphis worm on falling with tweezers, gently worm is fallen to being swept in culture dish with small size fur writing brush, binocular entity anatomical lens lower point length of time, in this process, gently sweep or picking wooly aphis, prevent its limb injury; After wooly aphis is swept and falls in culture dish, with 75% alcohol-pickled, and repeatedly rinse 3 times, the wax on wooly aphis is separated with aphid body with continuous wadding; Finally by each length of time wooly aphis put into respectively dactylethrae, stand-by by 75% soaking in alcohol 48 hours.
(3) wooly aphis ungrease treatment:
(a) wooly aphis of having divided the length of time is taken out from dactylethrae, prick an aperture at its belly with No. zero insect needle, and then be put into that in the dactylethrae that fills 75% alcoholic solution, to leave standstill 24h for subsequent use;
(b) after soaking and finishing, wooly aphis is taken out from alcoholic solution, and in culture dish, rinse 2-3 time with distilled water;
(c) wooly aphis is divided to be soaked in concentration the length of time be in 10% sodium hydroxide solution, and be placed in the insulating box of 37 DEG C and place 36h to polypide blushing transparent;
(d) wooly aphis is taken out from sodium hydroxide solution, with distilled water cleaning 3 times, and again prick another aperture with No. zero insect needle at its belly; Then by wooly aphis by transferring to the length of time in the dactylethrae that 75% alcoholic solution is housed, again leave standstill transparent to polypide till.
(4) making of slide sample:
(a) clean: from dactylethrae, taking out degreasing is better the transparent wooly aphis of polypide, be put on concave slide, with distilled water by clean alcoholic solution rinsing;
(b) dyeing: wooly aphis is put on concavity slide, water is exhausted with blotting paper, use 0.5% BG coloring agent to dye, after 10 minutes, coloring agent is rinsed well with distilled water; If polypide is not caught color after water cleans, and may be because polypide does not clean up, wooly aphis is repeated after (a) step, then carry out staining procedure one time;
(c) whole appearance: draw a wooly aphis of having dyeed with suction pipe, be placed on slide, and add a distilled water, then slide is put under binocular entity anatomical lens, to the whole appearance of polypide, make its sufficient limb not block aphid body, in order to avoid affect the observation in wax gland hole with No. zero insect needle;
(d) dry: along the whole appearance direction of polypide, water is blotted with blotting paper, then by standing polypide 5min;
(e) compressing tablet: with Huo Shi mounting medium making compressing tablet;
(f) de-soak is transparent: the slide making is put in to alcolhol burner flame envelope place, heats 30 seconds, dry to Huo Shi mounting medium and solidify and the bubble in slide is driven away;
(g) in placement Riker mount, preservation is stand-by: first slide sample is kept flat, treat that mounting medium inserts in Riker mount after having done completely again.
4, slide sample is observed and data analysis:
Make better if select, wax gland hole more clearly 1-4 age aphid, become each 5 pieces of aphid slide sample, number and the regularity of distribution thereof of under binocular stereomicroscope, observing its wax gland hole and aperture, record data are also analyzed; Experimental data is processed with Excel, and utilized the multiple ratio of SPSS17.0 statistics software each group of experimental data to be carried out to multiple ratio.
Embodiment 2:
Difference wooly aphis Morphological Identification feature in the length of time is as shown in table 1:
If table 1 wooly aphis aphid morphological feature is differentiated
Embodiment 3:
If under binocular stereomicroscope, observe wax gland hole wooly aphis 1-4 aphid in age to the characteristic distributions becoming on aphid body wall:
If wooly aphis 1-4 aphid in age is to becoming to have 4 row wax gland holes on aphid its body wall and be arranged in parallel, the 2nd row and the 3rd row of 14 joint body segments all respectively have a wax gland hole, and first and the 4th row in, 2,3 joints of head and Section 8 of belly do not have wax gland hole, all the other body segments respectively have a wax gland hole, amount to 50 wax gland holes, distributional pattern is roughly as shown in table 2:
If table 2 wax gland hole is wooly aphis 1-4 aphid in age and become the arrangement mode on aphid body wall
Embodiment 4:
If observe wooly aphis 1-4 aphid in age under binocular stereomicroscope, become each wax gland hole of aphid to be plum blossom flap by a lot of wax glands hole aperture to rearrange, and the little number of perforations in wax gland hole is not etc.; Record the little hole count in wax gland hole between different lines, and carry out analyzing between the aperture ordered series of numbers of wax gland hole, found that:
If the little hole count in wax gland hole of wooly aphis 1-4 aphid in age and one-tenth aphid side first row and the 4th row is obviously more than middle two, three row, and difference is extremely remarkable; Relatively, difference is not remarkable for first row and the 4th row, and relatively, difference is not remarkable yet for secondary series and the 3rd row.If if the first, fourth little hole count in row wax gland hole of wooly aphis aphid in 2 age obviously more than 1 age aphid, and significant difference, and 1,2 age wooly aphis two, three row wax gland hole apertures to count difference not remarkable.If if wooly aphis aphid in 4 age and the first, fourth little hole count in row wax gland hole that becomes aphid more than 3 age aphid, but difference is not remarkable.And if wooly aphis aphid in 3 age is to two, three row wax gland hole these indifferences of aperture base that become aphid, and aperture number is basic identical.
Result is as shown in table 3:
If table 3 wooly aphis 1-4 aphid in age and the one-tenth each row wax gland of aphid hole aperture are counted comparison
Above-mentioned result of the test shows, the little number of perforations in wax gland hole is not changeless in wooly aphis growth and development process, and one, the four little hole counts in row wax gland hole change with increasing the length of time, but that two, three row change is little.Meanwhile, if 4 age aphid and become the cured glandular orifice aperture number difference of aphid one to four row not remarkable, this explanation to the 4th age wooly aphis wax gland hole substantially broken up complete.
Embodiment 5:
Cured glandular orifice aperture in the ranks between the identical worm state of wooly aphis is counted to comparison, and carries out analyzing between the aperture ordered series of numbers of wax gland hole:
If table 4 wooly aphis aphid wax gland in 1 age hole aperture number comparison
If table 5 wooly aphis aphid wax gland in 2 age hole aperture number comparison
If table 6 wooly aphis aphid wax gland in 3 age hole aperture number comparison
If table 7 wooly aphis aphid wax gland in 4 age hole aperture number comparison
Table 8 wooly aphis becomes the aperture number comparison of aphid wax gland hole
As shown in table 4-8: the little number of perforations in wooly aphis wax gland hole is between 4-16, each little hole count in body segment wax gland hole is on average between 2-12, on body wall, be substantially symmetric, each body segment little number of perforations of glandular orifice of waxing is substantially equal accordingly for central authorities' two row (second and third row), and each body segment little number of perforations of glandular orifice of waxing is substantially equal accordingly for both sides row (first, fourth row).Wooly aphis by head, chest, abdomen three parts totally 14 body segments form, the four little number of perforations in row wax gland hole of head first segment are substantially equal, the 2nd, 3 liang of joint both sides row do not have wax gland hole, central two column number are suitable.From the 1st body segment of chest to the 8th body segment of belly (except the body segment without wax gland hole), the little number of perforations in wax gland hole of each body segment both sides row is all more than the central two little number of perforations in row wax gland hole, and significant difference.
Embodiment 6:
The different worm states of wooly aphis in the ranks cured glandular orifice aperture are counted comparison, and carry out the little hole count in wax gland hole and in the ranks analyze:
If table 9 wooly aphis 1-4 aphid in age and the one-tenth each row wax gland of aphid hole aperture number comparison
From table 9 relatively:
(1) if wooly aphis 1-4 aphid in age and become aphid head 3 joints and the little hole count in belly Section 8 wax gland hole all extremely significantly lower than the little hole counts in wax gland hole of 7 joints before chest 3 joints and belly, if if 2 age each body segment of aphid wax gland hole aperture number than 1 age aphid slightly increase.
(2) wooly aphis the wax gland hole aperture number great majority that increase each body segment along with worm age slightly increased to becoming aphid to compare 3 ages, but difference is not obvious.Wherein, if the wax gland hole aperture number that becomes aphid head 2,3 joints than 3,4 age aphid wax gland hole aperture number showed increased, and that other respectively save wax gland hole aperture number of variations is little.Wooly aphis becomes the every body segment wax gland of aphid hole aperture to count mean value substantially higher than 3,4 ages.
(3) the little hole count in wooly aphis belly 4-7 joint wax gland hole becomes many gradually, 14 body segment midriff Section 8 minimum number, wooly aphis is except the body segment of head and belly Section 8, from chest second section to belly Section seven, the little number of perforations in wax gland hole of each body segment both sides row is all more than the central two little number of perforations in row wax gland hole, and significant difference.In sum, the little number of perforations in wooly aphis wax gland hole is more flourishing at chest and belly, and be listed as 4-7 joint on belly side two the most flourishing, and continuous wadding is discharged at most in visible wooly aphis belly 4-7 joint both sides.
Embodiment 7:
If utilize four row wax gland holes on binocular entity microscope observing wooly aphis aphid body wall in two ages, as shown in Figure 1:
Each wax gland hole is plum blossom flap by a lot of wax glands hole aperture and rearranges, and the little number of perforations in wax gland hole is not etc., the body segment significant difference of chest and belly central authorities, the little hole count in wax gland hole starts to become gradually many from belly, at most, one, four row are particularly remarkable for belly 4-7 joint.
From embodiment of the present invention 2-7: if 1 age wooly aphis aphid to becoming aphid, on its body wall, having 4 row wax gland holes is arranged in parallel, both sides row head the 2nd, 3 joints and belly Section 8 are without wax gland hole, and all the other body segments and central authorities' each body segment of two row all have a wax gland hole, totally 50.Each wax gland hole is plum blossom flap by a lot of wax glands hole aperture and rearranges, and the little number of perforations in wax gland hole is not etc., experiment is observed and is found, if 1,2 age wooly aphis aphid 1,4 row wax gland hole aperture digital display works more than 2,3 row, but 1,4 row differences are not remarkable, 2,3 row differences are not remarkable yet; Experiment also find, if if the 2 little hole counts in the each body segment wax gland of aphid in age hole generally more than 1 age aphid; Cephalothorax abdomen is in totally 14 body segments, and the little hole count in wax gland hole starts to become gradually many from belly, and at most, 1,4 row are particularly remarkable for belly 4-7 joint, therefore can judge, the silk floss wadding of row wax gland hole, wooly aphis belly both sides secretion at most.
The present invention also comprises the change, displacement and the various substitute equivalents that fall in the scope of the invention.Here the example providing is only illustrative, instead of limitation of the present invention.
Claims (6)
1. a wooly aphis wax gland hole observational technique, is characterized in that, comprises the following steps:
(1) sample collection: in the wooly aphis harm phase in 5～June, the branch with wooly aphis is cut in choosing, cuts apple leaf and only leaves petiole, the apple stem cutting of handling well is entered to be equipped with in the 1000mL beaker of 2/3rds water, beaker mouth is sealed with gauze, be put in shady and cool ventilation place for subsequent use;
(2) wooly aphis is just processed: choose a wooly aphis worm and fall, remove covering the silk floss wadding of worm on falling, with writing brush, worm is fallen to being swept in culture dish and offer an explanation the length of time under anatomical lens, be that 70-90% alcohol rinses wooly aphis 3 times repeatedly by volumetric concentration, then by each length of time wooly aphis put into respectively dactylethrae, be that 70-80% soaking in alcohol 24 hours is above stand-by by volumetric concentration;
(3) wooly aphis ungrease treatment: the wooly aphis in the different length of times in step 2 is taken out from dactylethrae, after its belly is pricked an aperture, be that in 70-80% alcohol soak 24-48h afterflush clean in volumetric concentration with insect needle, in the sodium hydroxide solution that is 8-15% in mass concentration again, constant temperature leaves standstill to polypide blushing transparent, distilled water clean after again prick another aperture at belly, in volumetric concentration be in 70-80% alcoholic solution, again leave standstill to polypide transparent;
(4) making of wooly aphis slide sample: get polypide transparent in step 3 and be put on concave slide, with distilled water flushing 3-5 time, blotting paper by after water exhaustion with the coloring agent 5-15 minute that dyes, then coloring agent is rinsed well with distilled water; Polypide through whole appearance, dry leave standstill, compressing tablet, de-soak be put in Riker mount after transparent stand-by;
(5) slide sample is observed and data processing: if select 1-4 age aphid, become each 5 pieces of aphid slide sample, under binocular stereomicroscope, observe number and the regularity of distribution thereof of its wax gland hole and aperture, record data, experimental data is processed with Excel and is utilized SPSS17.0 statistics software to carry out multiple ratio to experimental data.
2. method according to claim 1, is characterized in that, in step 2, the alcohol concentration that described cleaning wooly aphis is used is 75%; The concentration of described immersion alcohol is 75%, and soak time is 24-48h.
3. method according to claim 1, is characterized in that, in step 2, described writing brush is small size, fur writing brush; In step 3, described insect needle is No. zero insect needle.
4. method according to claim 1, is characterized in that, the ungrease treatment process described in step 3 comprises the following steps:
(a) wooly aphis of having divided the length of time is taken out from dactylethrae, prick an aperture at its belly with No. zero insect needle, and then be put into that in the dactylethrae that fills 75% alcoholic solution, to leave standstill 24-48h for subsequent use;
(b) after soaking and finishing, wooly aphis is taken out from alcoholic solution, and in culture dish, rinse 2-3 time with distilled water;
(c) wooly aphis is divided to be soaked in concentration the length of time be in 10% sodium hydroxide solution, and be placed in the insulating box of 37 DEG C and place 36-48h to polypide blushing transparent;
(d) wooly aphis is taken out from sodium hydroxide solution, with distilled water cleaning 3 times, and again prick another aperture with No. zero insect needle at its belly; Then by wooly aphis by transferring to the length of time in the dactylethrae that 75% alcoholic solution is housed, again leave standstill to till transparent.
5. method according to claim 1, is characterized in that, the preparation of specimen's process described in step 4 comprises the following steps:
(a) clean: from dactylethrae, taking out degreasing is better the transparent wooly aphis of polypide, be put on concave slide, with distilled water flushing 3-5 time by clean alcoholic solution rinsing;
(b) dyeing: wooly aphis is put on concavity slide, water is exhausted with blotting paper, use coloring agent dyeing after 5-15 minute, coloring agent is rinsed well with distilled water;
(c) whole appearance: with suction pipe draw a wooly aphis of having dyeed, be placed on slide, and added a distilled water, then slide is put under binocular entity anatomical lens, with No. zero insect needle to the whole appearance of polypide;
(d) dry: along the whole appearance direction of polypide, water is blotted with blotting paper, then by standing polypide 5min;
(e) compressing tablet: with Huo Shi mounting medium making compressing tablet;
(f) de-soak is transparent: the slide making is put in to alcolhol burner flame envelope place, heats about 30-50 second, dry to Huo Shi mounting medium and solidify and the bubble in slide is driven away;
(g) in placement Riker mount, preservation is stand-by: first slide is kept flat, treat that mounting medium inserts in Riker mount after having done completely again.
6. method according to claim 5, is characterized in that, in step b, described coloring agent is BG, and mass concentration is 0.5%, and dyeing time is 10 minutes.
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| CN106818651A (en) * | 2017-04-12 | 2017-06-13 | 青岛农业大学 | A kind of method for breeding of eriosoma lanigerum |
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| CN106818651A (en) * | 2017-04-12 | 2017-06-13 | 青岛农业大学 | A kind of method for breeding of eriosoma lanigerum |
| CN106818651B (en) * | 2017-04-12 | 2020-02-11 | 青岛农业大学 | Breeding method of woolly apple aphids |
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