CN103911294A - Liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm - Google Patents
Liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm Download PDFInfo
- Publication number
- CN103911294A CN103911294A CN201410088715.7A CN201410088715A CN103911294A CN 103911294 A CN103911294 A CN 103911294A CN 201410088715 A CN201410088715 A CN 201410088715A CN 103911294 A CN103911294 A CN 103911294A
- Authority
- CN
- China
- Prior art keywords
- ginseng
- sesame
- radix astragali
- liquid fermentation
- lucid ganoderma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm, and belongs to the field of traditional Chinese medicine biological fermentation. According to the liquid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm, a liquid fermentation culture medium is inoculated with lucid ganoderma strains preserved in PDA culture medium, wherein the liquid fermentation culture medium is made from ginseng and radix astragali water extracts; culturing is carried out at a temperature of 22 to 30 DEG C with a rotating speed of 100 to 180r/min for 10 to 20 days so as to obtain the ginseng-radix astragali-lucid ganoderma medical mycoplasm. The liquid fermentation culture medium is made from ginseng and radix astragali water extracts, no other chemical ingredient is added, so that the liquid fermentation culture medium is safe and harmless; ginseng and radix astragali are combined, so that drug property and effect deficiencies of ginseng and radix astragali are avoided, and application range is widened; and in addition, the obtained ginseng-radix astragali-lucid ganoderma medical mycoplasm contains active ingredients of ginseng, radix astragali, and lucid ganoderma because of lucid ganoderma fermentation, effective combination of the three traditional traditional Chinese medicine ingredients is realized, balance of drug property and effect is achieved, and biological activities of original medicinal materials are improved.
Description
Technical field
The invention belongs to Chinese medicine microorganism fermentation field, be specifically related to a seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process.
Background technology
Ginseng, the Radix Astragali are all Qi-tonifying drugs conventional in clinical treatment, and still, in the property of medicine, drug effect, the two exists difference.Ginseng sweet and slightly bitter taste and property are flat, and the tonifying Qi yin-nourishing of holding concurrently is kept and do not walked.Radix Astragali taste is sweet warm in nature, and the double sun of holding up of tonifying Qi is walked and do not keep.By both pairings, ginseng stilbene one is kept one and is walked, and negative and positive are taken into account, and logical benefit out and out, without rushing down, can be used for the disease of all deficiency of vital energy deficiencies, has powerful tonifying Qi and helps unit's effect.Medicinal fungi glossy ganoderma is the traditional rare medicinal herbs that has strengthening by means of tonics, strengthens the body resistance to consolidate the constitution, and modern pharmacology studies confirm that, glossy ganoderma, for improving memory, promotes sleep, strengthens body immunity, auxiliary tumor chemoradiotherapy, and the aspects such as liver protecting all have significant curative effect.
The two-way fermentation technique of Chinese medicine is medicinal fungi is seeded in Chinese medicine matrix, when fermentative production medicinal fungi, the enzyme producing can catalysis Chinese medicine chemical composition transform, improve or reduce chemical composition of Chinese materia medica content or produce new compound, biological activity, the toxicity of the medicinal fungal substance that obtains of fermentation are changed, or possess new activity.Because liquid fermenting is short compared with solid fermentation required time, large, the constant product quality of turnout, production process be easy to monitoring, batch production industrialization level is high, therefore two-way fermentation technique is applied to liquid fermenting and there is larger economy and social benefit in producing.The document of the liquid bidirectional fermentation of having reported is at present less, and wherein Chinese patent 200910218080.7 discloses a kind of optimum process condition of glossy ganoderma fermentation red ginseng decocting liquid and anti-fatigue active of its tunning of utilizing.Not yet have at present any document open taking glossy ganoderma as fermented bacterium, fermentation is with the two-way liquid fermentation process of Compositional type fermented substrate and the application of the anti-oxidant aspect of medicinal fungal substance thereof of 2 kinds of Chinese traditional medicine composition of ginseng-Radix Astragali.
Summary of the invention
The object of this invention is to provide a seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process, the method time is short, and the ginseng stilbene sesame mycoplasma obtaining has higher anti-oxidant activity.
The invention provides a seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process, the method comprises:
To be stored in Ganderma lucidum strain in PDA solid medium by 3mm × 3mm, in the liquid fermentation medium that to be seeded to Chinese medicine ginseng, water extraction of astragalus membranaceus be matrix, 2 every bottle is that 22～30 DEG C, rotating speed are to cultivate 10d～20d under 100～180r/min condition in temperature, obtains joining stilbene sesame medicinal fungal substance;
Described liquid fermentation medium is prepared as follows: Chinese medicine ginseng, the Radix Astragali are pulverized, cross 10 mesh sieves, take respectively ginseng 20～60g, the Radix Astragali 6～90g, both mix, 300～500ml distilled water heating and refluxing extraction 2～3 times, each 0.5h, merges water extraction liquid and is settled to 1L, pH nature, be sub-packed in 250ml triangular flask, every bottled liquid measure 50ml, sealing, 115～121 DEG C of sterilizing 20～30min.
Preferably, described Ganderma lucidum strain comprises red sesame and cold sesame.
Preferably, when described Ganderma lucidum strain is red sesame, in liquid fermentation medium, get ginseng 30g, Radix Astragali 10g.
Preferably, when described Ganderma lucidum strain is red sesame, leavening temperature is 24～26 DEG C, and rotating speed is 120～160r/min.
Preferably, when described Ganderma lucidum strain is red sesame, fermentation time is 15d～17d.
Preferably, when described Ganderma lucidum strain is cold sesame, in liquid fermentation medium, get ginseng 30g, Radix Astragali 15g.
Preferably, when described Ganderma lucidum strain is cold sesame, leavening temperature is 26～28 DEG C, and rotating speed is 120～160r/min.
Preferably, when described Ganderma lucidum strain is cold sesame, fermentation time is 15d.
Beneficial effect of the present invention
The invention provides a seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process, the liquid fermentation medium that the present invention uses is ginseng, the composite Chinese traditional medicine water extract of the Radix Astragali, adds without other chemical compositions, safe and harmless; The combination that people is participated in to the Radix Astragali will make up the deficiency of the property of medicine, drug effect separately, expand the scope of indication, and under the fermentation of glossy ganoderma, in the medicinal fungal substance obtaining, there is the activeconstituents of ginseng, the Radix Astragali, glossy ganoderma simultaneously, realize effective compatibility of 3 taste traditional Chinese medicines, pharmacological property balance, the biological activity of lifting crude drug.Gained fermented liquid and mycelium are separated to methanol solution supersound extraction after fermented liquid freeze-drying; Mycelium crushed after being dried, 70% ethanol ultrasonic extraction, remove ultra-oxygen anion free radical and hydroxyl radical free radical with fermentation broth extract and mycelium extract respectively, when result shows 10mg/ml, red sesame fermented liquid to the clearance rate of superoxide anion and hydroxyl radical free radical for being not less than 70% and 50%; Red camphorata mycelium is all not less than 30% to the clearance rate of superoxide anion and hydroxyl radical free radical; Cold sesame fermented liquid is not less than the clearance rate of superoxide anion and hydroxyl radical free radical and 45% and 50%; Cold camphorata mycelium is not less than 50% and 10% to the clearance rate of superoxide anion and hydroxyl radical free radical, and the ginseng stilbene sesame medicinal fungal substance that visible the inventive method makes has stronger anti-oxidant activity.
Embodiment
Below in conjunction with specific embodiment, the present invention is done to further detailed description.
Comparative example 1
Chinese medicine ginseng, the Radix Astragali are pulverized, crossed 10 mesh sieves, take respectively ginseng and Radix Astragali 20g, 30g, 40g, 50g, 60g, add respectively 500ml distilled water heating and refluxing extraction 2 times, each 0.5h, merges water extraction liquid and is settled to 1L, pH nature, is sub-packed in 250ml triangular flask every bottled liquid measure 50ml.Sealing, 121 DEG C of sterilizing 20min, cooling for subsequent use.To be stored in the red sesame in PDA solid medium, cold sesame bacterial classification by 3mm × 3mm, be seeded in the liquid fermentation medium that Chinese medicine ginseng or water extraction of astragalus membranaceus are matrix, 2 every bottle, under 26 DEG C, 150r/min condition, cultivate 12d, fermented liquid after filtration, and rinses three times repeatedly, filters, dry, obtain mycelium.The mycelia scale of construction data that comparative example 1 obtains are as shown in table 1:
Table 1
The mycelia scale of construction of table 1 for fermenting red sesame according to concentration fermented substrate in difference, trembling with fear after sesame 12d, as can be seen from Table 1: water extraction of astragalus membranaceus is discontented with the demand of pure gold sesame, cold sesame growth, and the mycelia scale of construction is less; Ginseng aqueous extract is suitable for growth demand, and especially, in the time that concentration in ginseng aqueous extract is 30g～60g/L, the accumulation of bacterial classification mycelial biomass is higher.So the main matrix using 30～60g/L ginseng aqueous extract (the especially ginseng aqueous extract of 30g/L) in composite Chinese medicine matrix, is adding water extraction of astragalus membranaceus wherein, forms composite herb fermenting matrix.
Embodiment 1
Chinese medicine ginseng, the Radix Astragali are pulverized, crossed 10 mesh sieves, take 5 parts of 30g ginsengs, take respectively again Radix Astragali 6g, 10g, 15g, 30g, 60g, 90g, mix with ginseng respectively, add 500ml distilled water heating and refluxing extraction 2 times, each 0.5h, merge water extraction liquid and be settled to 1L, pH nature, is sub-packed in 250ml triangular flask, every bottled liquid measure 50ml, sealing, 121 DEG C of sterilizing 20min, cooling for subsequent use.To be stored in the red sesame in PDA solid medium, cold sesame bacterial classification by 3mm × 3mm, in the liquid fermentation medium that to be seeded to Chinese medicine ginseng, the full medicinal material water extraction of Radix Astragali liquid be matrix, 2 every bottle, under 26 DEG C, 150r/min condition, cultivate 12d, fermented liquid after filtration, and rinses three times repeatedly, is filtering, dry, obtain joining stilbene sesame property of medicine mycelium.The mycelia scale of construction data that embodiment 1 obtains are as shown in table 2:
Table 2
Embodiment 2
Chinese medicine ginseng, the Radix Astragali are pulverized, crossed 10 mesh sieves, take ginseng 30g, Radix Astragali 10g is red sesame fermented substrate; Take ginseng 30g, Radix Astragali 15g, for cold sesame Zymocyte, adds respectively 500ml distilled water heating and refluxing extraction 2 times, and each 0.5h merges water extraction liquid and is settled to 1L, and pH nature, is sub-packed in 250ml triangular flask every bottled liquid measure 50ml.Sealing, 121 DEG C of sterilizing 20min, cooling for subsequent use.To be stored in the red sesame in PDA solid medium, cold sesame bacterial classification by 3mm × 3mm, in the liquid fermentation medium that to be seeded to Chinese medicine ginseng, the full medicinal material water extraction of Radix Astragali liquid be matrix, 2 every bottle, adjusting temperature is 22 DEG C, 24 DEG C, 26 DEG C, 28 DEG C, 30 DEG C, under 150r/min condition, cultivates 12d, and fermented liquid after filtration, and repeatedly rinse three times, filtering, dry, obtain joining stilbene sesame property of medicine mycelium.The mycelia scale of construction data that embodiment 2 obtains are as shown in table 3:
Table 3
Embodiment 3
Chinese medicine ginseng, the Radix Astragali are pulverized, crossed 10 mesh sieves, take ginseng 30g, Radix Astragali 10g is red sesame fermented substrate; Take ginseng 30g, Radix Astragali 15g, for cold sesame Zymocyte, adds respectively 300ml distilled water heating and refluxing extraction 3 times, and each 0.5h merges water extraction liquid and is settled to 1L, and pH nature, is sub-packed in 250ml triangular flask every bottled liquid measure 50ml.Sealing, 121 DEG C of sterilizing 20min, cooling for subsequent use.To be stored in the red sesame in PDA solid medium, cold sesame bacterial classification by 3mm × 3mm, in the liquid fermentation medium that to be seeded to Chinese medicine ginseng, the full medicinal material water extraction of Radix Astragali liquid be matrix, 2 every bottle, adjust rotating speed to 100,120,140,160,180r/min, under 26 DEG C of conditions, cultivate 12d, fermented liquid after filtration, and repeatedly rinse three times, filtering, dry, obtain joining stilbene sesame property of medicine mycelium.The mycelia scale of construction data that embodiment 3 obtains are as shown in table 4:
Table 4
Embodiment 4
Chinese medicine ginseng, the Radix Astragali are pulverized, crossed 10 mesh sieves, take ginseng 30g, Radix Astragali 10g is red sesame fermented substrate; Take ginseng 30g, Radix Astragali 15g, for cold sesame Zymocyte, adds respectively 500ml distilled water heating and refluxing extraction 2 times, and each 0.5h merges water extraction liquid and is settled to 1L, and pH nature, is sub-packed in 250ml triangular flask every bottled liquid measure 50ml.Sealing, 121 DEG C of sterilizing 20min, cooling for subsequent use.To be stored in the red sesame in PDA solid medium, cold sesame bacterial classification by 3mm × 3mm, in the liquid fermentation medium that to be seeded to Chinese medicine ginseng, the full medicinal material water extraction of Radix Astragali liquid be matrix, 2 every bottle, 26 DEG C, the fermentation of 150r/min condition, get and cultivate 11d, 13d, 15d, 17d, 19d fermentation culture bottle, after filtration, repeatedly rinse separate fermentation liquid and mycelium.Methanol solution supersound extraction after fermented liquid freeze-drying; Mycelium crushed after being dried, 70% ethanol ultrasonic extraction, removes ultra-oxygen anion free radical and hydroxyl radical free radical with fermentation broth extract and mycelium extract respectively.Data are as shown in table 5:
Table 5
The explanation of above embodiment is just for helping to understand method of the present invention and core concept thereof.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of the claims in the present invention.
To the above-mentioned explanation of the disclosed embodiments, make professional and technical personnel in the field can realize or use the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiment, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (8)
1. a seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process, is characterized in that, the method comprises:
To be stored in Ganderma lucidum strain in PDA solid medium by 3mm × 3mm, in the liquid fermentation medium that to be seeded to Chinese medicine ginseng, water extraction of astragalus membranaceus be matrix, 2 every bottle is that 22～30 DEG C, rotating speed are to cultivate 10d～20d under 100～180r/min condition in temperature, obtains joining stilbene sesame medicinal fungal substance;
Described liquid fermentation medium is prepared as follows: Chinese medicine ginseng, the Radix Astragali are pulverized, cross 10 mesh sieves, take respectively ginseng 20～60g, the Radix Astragali 6～90g, both mix, 300～500ml distilled water heating and refluxing extraction 2～3 times, each 0.5h, merges water extraction liquid and is settled to 1L, pH nature, be sub-packed in 250ml triangular flask, every bottled liquid measure 50ml, sealing, 115～121 DEG C of sterilizing 20～30min.
2. according to the seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process shown in claim 1, it is characterized in that, described Ganderma lucidum strain comprises red sesame and cold sesame.
3. according to the seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process shown in claim 1, it is characterized in that, when described Ganderma lucidum strain is red sesame, in liquid fermentation medium, get ginseng 30g, Radix Astragali 10g.
4. according to the seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process shown in claim 1, it is characterized in that, when described Ganderma lucidum strain is red sesame, leavening temperature is 24～26 DEG C, and rotating speed is 120～160r/min.
5. according to the seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process shown in claim 1, it is characterized in that,, when described Ganderma lucidum strain is red sesame, fermentation time is 15d～17d.
6. according to the seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process shown in claim 1, it is characterized in that, when described Ganderma lucidum strain is cold sesame, in liquid fermentation medium, get ginseng 30g, Radix Astragali 15g.
7. according to the seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process shown in claim 1, it is characterized in that, when described Ganderma lucidum strain is cold sesame, leavening temperature is 26～28 DEG C, and rotating speed is 120～160r/min.
8. according to the seed ginseng stilbene sesame medicinal fungal substance liquid fermentation process shown in claim 1, it is characterized in that, when described Ganderma lucidum strain is cold sesame, fermentation time is 15d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410088715.7A CN103911294B (en) | 2014-03-11 | 2014-03-11 | One seed ginseng stilbene sesame medicinal fungal substance liquid fermentation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410088715.7A CN103911294B (en) | 2014-03-11 | 2014-03-11 | One seed ginseng stilbene sesame medicinal fungal substance liquid fermentation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103911294A true CN103911294A (en) | 2014-07-09 |
| CN103911294B CN103911294B (en) | 2016-05-11 |
Family
ID=51037302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410088715.7A Active CN103911294B (en) | 2014-03-11 | 2014-03-11 | One seed ginseng stilbene sesame medicinal fungal substance liquid fermentation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103911294B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110302129A (en) * | 2019-08-21 | 2019-10-08 | 云南澳玛生物技术有限公司 | One kind educating hair anticreep scalp nursing solution |
| CN113455287A (en) * | 2021-07-07 | 2021-10-01 | 重庆市酉阳县琦睿峰食用菌有限责任公司 | Method for directly separating morchella tissue by PDA (personal digital Assistant) plate to obtain pure strain |
| CN115463187A (en) * | 2018-08-24 | 2022-12-13 | 昆明思多尔生物科技有限公司 | Application of anticancer composition in preparation of medicine for treating cancer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706238A (en) * | 2004-06-11 | 2005-12-14 | 蒋丹 | Glossy ganoderma culturing process |
-
2014
- 2014-03-11 CN CN201410088715.7A patent/CN103911294B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706238A (en) * | 2004-06-11 | 2005-12-14 | 蒋丹 | Glossy ganoderma culturing process |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115463187A (en) * | 2018-08-24 | 2022-12-13 | 昆明思多尔生物科技有限公司 | Application of anticancer composition in preparation of medicine for treating cancer |
| CN110302129A (en) * | 2019-08-21 | 2019-10-08 | 云南澳玛生物技术有限公司 | One kind educating hair anticreep scalp nursing solution |
| CN113455287A (en) * | 2021-07-07 | 2021-10-01 | 重庆市酉阳县琦睿峰食用菌有限责任公司 | Method for directly separating morchella tissue by PDA (personal digital Assistant) plate to obtain pure strain |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103911294B (en) | 2016-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100494341C (en) | Huishuhua fermentation preparation process using Chinese herb medicine as medium | |
| CN103263448B (en) | Fermentation bacteria used for fermentation pretreatment to improve extraction of Ginkgo biloba L. leaf flavone and application | |
| CN105193905A (en) | Traditional Chinese medicine processing technology of ganoderma-astragalus membranaceus bidirectional fermentation | |
| CN102648724B (en) | Preparation method for vine tea | |
| CN109536560A (en) | A method of improving rare saponin content in ginseng water extract | |
| CN102242044A (en) | Cordyceps cicadae wine and preparation method thereof | |
| CN109528568A (en) | A kind of composition and preparation method thereof with anti-aging and white-skinned face function | |
| CN104068379A (en) | Health-care oral solution | |
| CN103976351B (en) | A kind of health food of develop immunitypty improving water flood and two-step fermentation preparation method thereof | |
| CN104846018A (en) | Cordyceps enzyme deep layer liquid fermentation and expanding culture method | |
| KR20150140550A (en) | Ginseng preparations containing abundant ginsenoside using a beauveria bassiana and method thereof | |
| CN103190547B (en) | Chinese herbal medicine feed additive for effectively controlling comprehensive respiratory diseases of pigs | |
| CN102206692B (en) | Process for increasing extraction amount of baicilin by using traditional Chinese medicine fermentation technology | |
| CN103815279B (en) | Red yeast rice rich in coenzyme Q10 and preparation method thereof | |
| CN103911294B (en) | One seed ginseng stilbene sesame medicinal fungal substance liquid fermentation method | |
| CN108185140A (en) | Pueraria lobata composite fermentation object and its preparation method and application | |
| CN103908489A (en) | Bidirectional solid fermentation method of ginseng-radix astragali-lucid ganoderma medical mycoplasm | |
| CN106047978B (en) | A method of rare ginsenoside CK is synthesized using saponin bioconversion | |
| CN105368695A (en) | Fermented vinegar with poria cocos and propolis and method for manufacturing fermented vinegar | |
| CN104877183A (en) | Ganoderma-antradiacomphora polysaccharide and membrane permeation preparation process thereof | |
| CN101104006A (en) | Method for preparing Chinese caterpillar fungus extracting concentrated liquid and application thereof | |
| CN101935602A (en) | Method for producing low-alcohol fermented beverage of eagle tea | |
| CN105213461A (en) | Utilize the two-way fermentation of Ganoderma-Radix Codonopsis to improve the biotechnology of its drug effect | |
| CN106071605A (en) | A kind of glossy ganoderma fermentation prepares the method for ferment | |
| CN105412189A (en) | Kefir fermentation scrophularia henryi hemsl powder and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |