CN103893051A - Preparation of enzymolysis ginseng and application thereof in cosmetics and foods - Google Patents
Preparation of enzymolysis ginseng and application thereof in cosmetics and foods Download PDFInfo
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Abstract
The invention relates to a preparation process of enzymolysis ginseng, and in particular relates to a novel ginseng processed product which can be used for increasing the contents of arginine monoglucoside (AF) and arginine diglucoside (AFG) in ginseng by virtue of a method of combining cellulase and beta-amylase; meanwhile, the invention further discloses effects of the enzymolysis ginseng in liver protection, ultraviolet ray and radiation resistance, and fatigue resistance. The enzymolysis ginseng disclosed by the invention is applicable to cosmetics and foods, and belongs to a Chinese medicinal herb processing technology and the application fields of healthcare foods and cosmetic.
Description
Technical field
The present invention relates to a kind of processing technology of enzymolysis Radix Ginseng, specifically adopt method that cellulase combines with beta amylase to increase the content of arginine monoglycosides (AF), arginine diglucoside (AFG) in Radix Ginseng, a kind of new processing of Panax ginseng product, also disclose simultaneously enzymolysis Radix Ginseng protecting the liver, effect of uvioresistant radioprotective and resisting fatigue, the enzymolysis Radix Ginseng of this patent invention can be applicable in cosmetics and food.Belong to processing technique of Chinese herbs, belong to health food, cosmetic applications field.
Background technology
Arginine derivative AF is the arginine and the product that reducing sugar (glucose, maltose) reacts in Radix Ginseng with AFG, is the intermediate product of mailland reaction, in [1,2] first finding in Radix Ginseng Rubra and identify at the end of last century.Pharmaceutical research shows that [3] amino acid derivativges AF, AFG have the skin of improvement quality, delay skin aging, suppressing TNF-α produces, suppress cyclooxygenase 2, defying age, promote the generation of I/IV collagen type, the effect that T-5398 activates, and can promote hyaluronic acid and NO to produce.Enzymolysis Radix Ginseng is a kind of processed goods of Radix Ginseng, and wherein more enzymolysis Radix Ginseng is not high for the content of amino acid derivativges AF, AFG.In enzymolysis process, add cellulase, the cell wall that cellulase can decompose plant makes more active substance stripping [4], the content of enzymolysis Ginsenosides in Panax Ginseng is also compared with enzymolysis Radix Ginseng is not many, and modern pharmacology shows that ginsenoside has antioxidation, whitening, antidotal effect.Beta amylase (Isosorbide-5-Nitrae-α-D-glucan maltohydrolase, EC 3.2.1.2) is a kind of circumscribed-type saccharifying enzyme, while acting on starch, can cut in turn next maltose unit from the non reducing end of α-Isosorbide-5-Nitrae glycosidic bond, produce maltose and macromolecular β-boundary dextrin [5].The primary product that beta amylase decomposes amylose is maltose, and the product that decomposes amylopectin is mainly maltose and macromolecular β-boundary dextrin, and this increase for AFG in enzymolysis Radix Ginseng provides material base.
Increase reducing sugar by Radix Ginseng enzymolysis at present, the research that the content of AF, AFG is increased is less, the invention discloses that a kind of processing technique and enzymolysis Radix Ginseng of preparing enzymolysis Radix Ginseng protects the liver, anti-ultraviolet radioprotective and fatigue-resisting function, and application in cosmetics, health food.
Summary of the invention
The invention discloses Radix Ginseng prepared by a kind of enzymatic isolation method, in enzymolysis Radix Ginseng, all more enzymolysis Radix Ginseng is not high for AF, AFG and total Ginsenosides Content.
The invention discloses the application of enzymolysis Radix Ginseng in cosmetics, health food.
radix Ginseng prepared by enzymatic isolation method disclosed by the invention, prepare by the following method:
1) get a certain amount of Radix Ginseng powder (80 order), regulate solid-liquid ratio 1:10～1:20, pH5～5.5, add cellulase by 400:1～500:1, enzymolysis 2～3h, 100 DEG C of deactivations;
2) regulate pH5.2～5.8, by 40g～50g:1 μ L(50 ten thousand u/mL) add beta amylase, high-temperature inactivation after enzymolysis 24～36h, by 70 DEG C of oven dry of the Radix Ginseng powder after enzymolysis, to obtain final product.
The application of enzymolysis Radix Ginseng of the present invention in food with effect of hepatic protection.
The application of enzymolysis Radix Ginseng of the present invention in anti-ultraviolet radiation cosmetics.
The application of enzymolysis Radix Ginseng of the present invention in resisting fatigue food.
the present invention's its good effect compared with Radix Ginseng is:
The prepared enzymolysis Radix Ginseng of method that adopts cellulase to combine with the common enzymolysis of beta amylase, has not only increased ginsenoside's amount, and has increased considerably the amount of arginine derivative AF and AFG.Have protect the liver, uvioresistant radioprotective and fatigue-resisting function, can be used for cosmetics and field of health care food.Operational approach of the present invention is simple, feasible, is applicable to large batch of suitability for industrialized production.
Below adopt high-efficient liquid phase technique to carry out performance rating to enzymolysis Radix Ginseng.
experimental example 1
technique of the present invention is prepared the analysis of arginine derivative AF, AFG in enzymolysis Radix Ginseng
1.1 experiment materials and method
1.1.1 experiment material
Radix Ginseng picks up from the 5 years fresh Radix Ginsengs in Hunchun; It is that raw material is made by technique of the present invention that enzymolysis Radix Ginseng adopts Radix Ginseng powder; AF, AFG mark product are the self-control of this laboratory, purity >98%; Phenyl isothiocyanate (PITC) and triethylamine (TEA), chromatographically pure, being more than Ai Jieer company of the U.S. provides; Chromatographic grade normal hexane, Tianjin recovery fine chemistry industry institute; Chromatograph methanol and chromatograph acetonitrile, U.S. Tedia; Other conventional chemical reagent (analytical pure), Beijing Chemical Plant.
1.1.2 experimental technique
1.1.2.1 HPLC-UV analysis condition
The Japanese Shimadzu of Shimadzu LC-20A high performance liquid chromatograph (SPD-20A UV-detector, LC-20AT pump, CTO-10AS VP column oven, LC-solution work station) company.KQ-250DB type ultrasonic cleaner (Kunshan ultrasonic instrument company limited); Venusil-AA amino acid analysis dedicated columns (5 μ m, 4.6mm × 250mm), 40 DEG C of column temperatures; Detect wavelength 254nm; Mobile phase A: sodium acetate buffer solution (pH6.5)-acetonitrile solution; Mobile phase B: acetonitrile-water (volume ratio is 4:1) solution; Linear gradient elution program: 0.0min, 0%B; 4min, 3%B; 16min, 10%B; Flow velocity 1.0mL/min.
1.1.2.2 the preparation of reference substance solution is with derivative
Accurately take AF standard substance 0.015g water dissolution and be settled to 10mL, get 1mL and be placed in 2mL centrifuge tube, add triethylamine acetonitrile solution 100 μ L, phenyl isothiocyanate acetonitrile solution 50 μ L, mix, room temperature is placed 1h, then add normal hexane 400 μ L, after jolting, place 10min, take off a layer solution, 0.2 μ m membrane filtration, gets 20 μ L and carries out chromatography.
Accurately take AFG standard substance 0.015g water dissolution and be settled to 10mL, get 1mL and be placed in 2mL centrifuge tube, below derivative step is the same, room temperature is placed 1h, then adds normal hexane 400 μ L, places 10min after jolting, take off a layer solution, 0.2 μ m membrane filtration, gets 20 μ L and carries out chromatography.
1.1.2.3 the preparation of sample solution is with derivative
Accurately take Radix Ginseng, enzymolysis Radix Ginseng powder (40 order) 1.0g, add respectively 20mL methanol soaked overnight, filter, residue adds 20mL water, (250W, 100kHZ) extract 3 times, each 30min, filters, merging filtrate, low pressure rotation is concentrated into dry, and distilled water water dissolution moves in 10mL volumetric flask, standardize solution.Getting 1mL with pipettor respectively adds in 2mL centrifuge tube, add respectively triethylamine acetonitrile solution 100 μ L, phenyl isothiocyanate acetonitrile solution 100 μ L, mix, room temperature is placed 1h, then add normal hexane 400 μ L, 10min is evenly placed in jolting, takes off a layer solution, 0.2 μ m membrane filtration, gets 20 μ L sample introductions and analyzes (n=3).
1.2 HPLC-UV analysis results
Result HPLC-UV(of arginine derivative AF and AFG from Radix Ginseng and enzymolysis Radix Ginseng is shown in accompanying drawing 1, accompanying drawing 2) known, with Radix Ginseng comparison, in enzymolysis Radix Ginseng, arginine derivative AF and AFG have increased respectively 2.3%, 84.5%.
experimental example 2
the present invention prepares the detection of total saponin content in enzymolysis Radix Ginseng
2.1 experiment materials and method
2.1.1 experiment material
Radix Ginseng picks up from the 5 years fresh Radix Ginsengs in Hunchun; It is that raw material is made by technique of the present invention that enzymolysis Radix Ginseng adopts Radix Ginseng powder; Standard substance ginsenoside Rb1, Rb2, Rc, Rd, Re, Rf, Rg
1, Rb3 is purchased from Nat'l Pharmaceutical & Biological Products Control Institute; Chromatograph acetonitrile, U.S. Tedia; Shimadzu LC-20A high performance liquid chromatograph (SPD-20A UV-detector, LC-20AT pump, CTO-10AS VP column oven, LC-solution work station), Japanese Shimadzu company
2.1.2 experimental technique
2.1.2.1 the preparation of sample to be tested
Accurately take respectively the each 1.0g of Radix Ginseng powder (crossing 80 mesh sieves) after Radix Ginseng, enzymolysis, be placed in tool plug conical flask, add 50mL methanol, supersound extraction (250W, 100kHZ) extract 2 times, each 30min, filters, merging filtrate, concentrating under reduced pressure, and by methanol constant volume to 25mL volumetric flask, shake up, cross 0.2 μ m filter membrane, treat that HPLC-UV measures.
2.1.2.2 the foundation of HPLC analysis condition
Yi Lite Hypersil ODS2(4.6 mm × 250 mm, m) chromatographic column of 5 μ; Mobile phase: acetonitrile and water gradient elution, 0-24min, 18.5-22% acetonitrile; 24-26min, 26-32% acetonitrile; 30-50min, 32-33.5% acetonitrile; 50-55min, 33.5-38% acetonitrile; 55-65min, 38-40%.Flow velocity 1mL/min; Detect wavelength: 203nm; Column temperature: 30 DEG C; Sample size 20 μ L.
2.2 experimental result
In Radix Ginseng and enzymolysis Radix Ginseng, saponin content the results are shown in Table 1, and its conventional Radix Ginseng total saponins is respectively 16.7mg/g, 19.3mg/g.Radix Ginseng and enzymolysis Radix Ginseng HPLC-UV figure (seeing accompanying drawing 3,4)
Content of ginsenoside in table 1 Radix Ginseng and enzymolysis Radix Ginseng
| Ginsenoside | Radix Ginseng (mg/g) | Enzymolysis Radix Ginseng (mg/g) |
| Rg1 | 5.9 | 6.3 |
| Re | 2.1 | 1.7 |
| Rf | 3 | 3.6 |
| Rb1 | 1.9 | 2.4 |
| Rc | 1.13 | 1.43 |
| Rb2 | 1.3 | 1.6 |
| Rb3 | 0.77 | 1.1 |
| Rd | 0.58 | 1.23 |
experimental example 3
the present invention prepares the effect that enzymolysis Radix Ginseng protects the liver
3.1 experiment materials, reagent and instrument
Radix Ginseng picks up from the 5 years fresh Radix Ginsengs in Hunchun; It is that raw material is made by technique of the present invention that enzymolysis Radix Ginseng adopts Radix Ginseng powder; Bifendate drop pill, Beijing XieHe medicine Factory; SpectraMax Plus384 continuous spectrum scan-type enzyme, molecule instrument company of the U.S.; 96 hole ELISA Plate, Corning Incorporated; Total superoxide dismutase (T-SOD) testing cassete, Bioengineering Research Institute is built up in Nanjing; Malonaldehyde (MDA) test kit, Bioengineering Research Institute is built up in Nanjing; Coomassie brilliant blue test kit, Bioengineering Research Institute is built up in Nanjing; ALT test kit, Bioengineering Research Institute is built up in Nanjing; AST test kit, Bioengineering Research Institute is built up in Nanjing; Carbon tetrachloride, Tianjin Fu Yu Fine Chemical Co., Ltd; Sodium carboxymethyl cellulose, Ke Miou chemical reagent development centre, Tianjin; The imperial fish soybean oil of gold, beneficial Hai Jiali (Harbin) oil and foodstuffs industry is limited; FS-2 tissue refiner, Community of Jin Tan County Jin Cheng Guo Sheng experimental apparatus factory;
3.2 test methods:
3.2.1 zoopery
5～6 week age 50 of ICR male mices, body weight 20 ± 2g, purchased from the Bethune of Jilin University medical college Experimental Animal Center, credit number SCXK-(Ji) 2007-0003.24 ± 1 DEG C of receptacle temperature, humidity 50 ± 5%, mice freely ingests and drinks water, and adaptability is raised one week.
3.2.2 test method
Mice adaptability is divided into 5 groups at random by body weight after feeding: blank group, model group, positive group, Radix Ginseng group, enzymolysis Radix Ginseng group, 9 every group.Each group dosage is respectively: positive drug bifendate group, 150mg/kg, Radix Ginseng group, 900mg/kg; Enzymolysis Radix Ginseng group, 900mg/kg.Radix Ginseng, enzymolysis Radix Ginseng are pulverized respectively, cross 120 mesh sieves, respectively organize medicine with 5 ‰ sodium carboxymethyl cellulose (CMCNa) solution suspending, respectively gastric infusion, and gavage volume 0.01mL/g, blank group, model group gavage are given equal-volume CMCNa solution.Mice 8:00 in morning every day gastric infusion, continuously gavage 15 days, after last administration 1 hour, except blank group, all the other each groups were pressed 10mL/kg lumbar injection 0.1%CCL4 soybean oil solution.After fasting 6 hours, mice is plucked eyeball and gets blood, and separation of serum is put 4 DEG C and saved backup.Cut open the belly immediately and get liver and spleen, with the normal saline flushing surface bloodstain of 4 DEG C of pre-coolings, filter paper is wiped dry, weighs, and gets 0.5g hepatic tissue, adds 0.9% normal saline homogenate of 9 times of volumes of pre-cooling simultaneously, the centrifugal 10min of 4500rpm, and supernatant is put 4 DEG C and is saved backup.
ALT in mice serum, the mensuration of AST vigor are carried out 96 orifice plate application of samples in strict accordance with the operating procedure of test kit description, and microplate reader is measured, and calculates AST in mouse blood, ALT content (the results are shown in Table 2); In mouse liver, the mensuration of MDA and SOD adopts 96 orifice plate application of samples in strict accordance with the operating procedure of test kit description, and microplate reader is measured, and calculates MDA in mouse liver tissue, SOD level (the results are shown in Table 3).
3.2.3 statistical method
Data result represents with mean+SD (X ± S).Carry out statistical analysis with SPSS 17.0 softwares, use ANOVA variance analysis deal with data, * represent and significantly (P<0.05) of model group comparing difference, * * represents and significantly (P<0.01) of the poor heteropole of model group; # represents and significantly (P < 0.05) of normal group comparing difference, and ## represents and significantly (P < 0.01) of the poor heteropole of normal group.
The impact of table 2 enzymolysis Radix Ginseng on ALT, AST in mice serum
| Group | ALT(U/L) | AST(U/L) |
| Blank group | 5.02±0.9 | 5.9±1.0 |
| Model group | 25.9±6.1 | 14.7±4.3 |
| Bifendate drop pill group (150mg/kg) | 10.4±3.7 | 9.6±0.8 |
| Radix Ginseng group (900mg/kg) | 18.9±2.1﹡﹡ ,﹟﹟ | 10.8±1.5﹡﹡ ,﹟﹟ |
| Enzymolysis Radix Ginseng group (900mg/kg) | 15.8±2.7﹡﹡ ,﹟﹟ | 10.1±1.1﹡﹡ ,﹟﹟ |
Note: with blank group Xiang be P < 0.01 Bi ﹡ ﹡; With model group Xiang be P < 0.01 Bi ﹟ ﹟.
The impact of table 3 enzymolysis Radix Ginseng on SOD, MDA in mouse liver tissue
| Group | MDA(mmol/mgprol) | AST(U/mgprol) |
| Blank group | 5.9±1.8 | 227±45 |
| Model group | ?20.4±3.5 | 162.8±75.6 |
| Bifendate drop pill group (150mg/kg) | 12.8±1.3 | ?208.7±21.2 |
| Radix Ginseng group (900mg/kg) | 14.9±0.6﹡﹡ ,﹟﹟? | 195.1±52.6﹡﹡ ,﹟﹟ |
| Enzymolysis Radix Ginseng group (900mg/kg) | 14.3±1.3﹡﹡ ,﹟﹟ | 207.7±60﹡﹡ ,﹟﹟ |
Note: with blank group Xiang be P < 0.01 Bi ﹡ ﹡; With model group Xiang be P < 0.01 Bi ﹟ ﹟.
3.3 conclusion
Experimental result is in table 2,3, can find out that people participates in enzymolysis Radix Ginseng group mice serum AST, ALT and have extremely significant difference (P < 0.01) compared with blank group, model group, and in enzymolysis Radix Ginseng group mice serum the level of AST, ALT significantly lower than Radix Ginseng group.People participates in enzymolysis Radix Ginseng group murine liver tissue SOD, MDA as can be seen from Table 2 has extremely significant difference (P < 0.01) compared with blank group, model group, and in enzymolysis Radix Ginseng group murine liver tissue the level of SOD, MDA significantly lower than Radix Ginseng group.
enzymolysis Radix Ginseng prepared by the present invention is in the radiation-resistant effect of uvioresistant
4.1 experiment materials, reagent and instrument
Radix Ginseng picks up from the 5 years fresh Radix Ginsengs in Hunchun; It is that raw material is made by technique of the present invention that enzymolysis Radix Ginseng adopts Radix Ginseng powder; Rutin is made by oneself by this laboratory, purity > 95%.TiO2 is nanoscale (Shanghai Jiang Hu group).Ethanol, chloroform, n-butyl alcohol, dimethyl sulfoxide (DMSO) etc. are analytical pure, Beijing Chemical Plant.JHBE-50S flash extracter, Henan Jin Nai Science and Technology Ltd..UV-2450 ultraviolet/visible spectrophotometer, Japanese SHIMADZU company.UV-5100 type ultraviolet/visible spectrophotometer, Shanghai Yuan Xi Instrument Ltd..EYELA series Rotary Evaporators, TOKYO RIKAKIKAI CO., LTD.BP211D electronic balance, German sartorius company.The automatic dual pure water distillator of SZ-93, Shanghai Yarong Biochemical Instrument Plant.
4.2 test method
4.2.1 the preparation of sample solution
Get respectively Radix Ginseng, enzymolysis Radix Ginseng powder (crossing 80 mesh sieves) 5g, add distilled water 30mL.Supersound extraction (250W, 100kHZ) is extracted 2 times, and each 30 minutes, filter, merging filtrate, standardize solution, in 100 mL volumetric flasks, is the Radix Ginseng extractive solution that crude drug concentration is 0.05g/mL, and diluted concentration is 0.025 g/mL.Rutin, the positive reference substance of TiO2, concentration is 0.025 g/mL.
4.2.2 the mensuration of absorbance
Successively at UV A district 400nm, 380nm, 360nm, 340nm, 320nm, UVB district 320nm, 300nm, 280nm, UVC district 280nm, 260nm, 246nm, 240nm, 220nm, 200nm, measure the light transmittance of each test liquid, parallel assay 3 times (n=3), calculates average absorption rate: A%=100%-T%.
4.3 results and conclusion
Pertinent literature shows [6], by ultraviolet spectrophotometry test absorbance size, is greater than 80% and can thinks to have anti-sunlight function, has stronger anti-sunlight function while being greater than 90%, has extraordinary spectrum anti-sunlight function in the time that absorptance approaches 100%.(accompanying drawing 5) result shows, Radix Ginseng, enzymolysis Radix Ginseng and positive reference substance rutin, the each test liquid UVC of TiO2 district absorbance are all lower than UVA, UVB.Wherein enzymolysis Radix Ginseng is high compared with Radix Ginseng at UVA, UVB, UVC section absorbance, and this explanation enzymolysis Radix Ginseng is compared with the good uvioresistant radiation-proof effect of having of Radix Ginseng.Nano TiO 2 is widely used in sun-proof skin-lightening cosmetic, but because the research of its safety is not enough deeply extensive, existing bibliographical information also reflects its limitation in cosmetic applications.Therefore the material that exploitation has a safer and more effective anti-sunlight function is necessary.
Enzymolysis Radix Ginseng prepared by the present invention has the effect of the body fatigue resistance of raising, and the enzymolysis Radix Ginseng food for exploitation with resisting fatigue effect has been cooked theoretical basis.
For further illustrating enzymolysis Radix Ginseng of the present invention in the application aspect resisting fatigue, further illustrate below by experiment in normal mouse body:
experimental example 5
the impact of enzymolysis Radix Ginseng on normal mouse anti-reflecting fatigue ability
5.1 experiment materials and reagent
Radix Ginseng picks up from the 5 years fresh Radix Ginsengs in Hunchun; It is that raw material is made by work of the present invention that enzymolysis Radix Ginseng adopts Radix Ginseng powder; Water storage barrel, purchased from the market of farm produce, Changchun; SHIMADZU-AUY220 type electronic balance, Japanese Shimadzu; Animal: 2 27 of monthly age ICR male mices, body weight 20 ± 2g, purchased from Jilin University's Experimental Animal Center.Every morning, sufficient drinking water was supplied with in 8 timings, 24 ± 1 DEG C of room temperatures, and adaptability is fed one week.
5.2 experimental technique
Be divided at random 3 groups: blank group, Radix Ginseng group, enzymolysis Radix Ginseng group, 9 every group.Each group dosage is respectively: Radix Ginseng group, 900mg/kg; Enzymolysis Radix Ginseng group, 900mg/kg.Radix Ginseng, enzymolysis Radix Ginseng are pulverized respectively, cross 120 mesh sieves, respectively organize medicine with 5 ‰ sodium carboxymethyl cellulose (CMCNa) solution suspending, gastric infusion respectively, and gavage volume 0.01mL/g, blank group gavage is given equal-volume CMCNa solution.Mice 8:00 in morning every day gastric infusion, continuous 2 weeks.Before administration every day, record Mouse Weight.After administration 2 weeks, take Mouse Weight, by 4/barrel, it is 25 ± 1 DEG C that mice is put into water temperature simultaneously, and the bucket went swimming that high 30cm is dark, records mice swimming time, exhausts as mice submerged 8s calculates swimming, can not coordination exercise.Each group numerical value screens with Grubbs criterion.
5.3 mouse swimming test results
Radix Ginseng group and enzymolysis Radix Ginseng group all can be swum the exhaustion time by significant prolongation mice, and wherein the effect of enzymolysis Radix Ginseng group is significantly greater than Radix Ginseng group.This experimental result shows that enzymolysis Radix Ginseng group can coordinate mice swimming time, has antifatigue effect (in table 4).
Each group of table 4 is on the mice impact of swimming exhaustion time
| Group | Dosage (mg/kg) | Number of animals | The weight of animals (g) | Swimming exhaustion time (min) | |
| Before administration | After | ||||
| Blank group | |||||
| 9 | 24.4 | 30.7 | ?11.6±4 | ||
| Radix Ginseng group | 900 | 9 | 23.8 | 30.01 | 24.7±10 ﹡﹡ |
| Enzymolysis Radix Ginseng group | 900 | 9 | 24.1 | 30.2 | 54±3.9 ﹡﹡ |
Note: ﹡ ﹡ P < 0.001
The present invention is taking enzymolysis Radix Ginseng extract as base substance, and contains acceptable carrier on one or more cosmetics, health food.The existence of these materials can maintain or strengthen the effect that technique of the present invention is prepared enzymolysis Radix Ginseng.In addition, what should particularly point out is, can be as required in the enzymolysis Radix Ginseng combination of invention, add one or more natural or synthetic other and this active substance to there is the composition of collaborative or assosting effect, these natural or synthetic auxiliary elements that may be added into be well known by persons skilled in the art with can imagine.Said compositions can be mixed with through external, cosmetics or food for oral administration, for skin protection skin moistening (comprising the skin of whole body), health food, cosmetics type is not limit, comprise emulsion, astringent, skin lotion, balance liquid, moisturiser, nutritional solution, nourishing cream, day cream, late frost, eye cream, massage cream, essence, cleansing milk, shampoo, hair care elite, bathing essence etc., and each confectionary, saccharide, beverage etc.Be suitable for cosmetics in order to prepare, cosmetic base is the needed material of daily cosmetics substrate, comprises surfactant oiliness composition used, and this extract joins the effect that can play whitening, reparation skin ultraviolet injury in cosmetics.To be suitable for healthcare food in order preparing, in health food, can to add the materials such as the food additive requiring in National Food Law.
List of references
[1] the firm man of Zheng, ginseng for medicinal use To is containing the research of ま れ Ru biological active substances に Seki The Ru, Love beautiful woman medical science (Japan), 1994,13 (2): 1-7
[2] Zheng Yinan, Song Puxing forever, Han Likun, Gao Jiuwu department, Xiang Lan, Testudinis Tian Jianzhi, Li Xianggao, Okuda Takudox. the separation of noval chemical compound in Radix Ginseng Rubra-arginine derivative and Structural Identification. Acta Pharmaceutica Sinica, 1996,31 (3): 191-195.
[3] the rugged large Gang of rock, all Yan Yang. the raw short Jin Elixirs of plain Productivity and び skin Skinization make-up material [p] are stopped up in anti-scorching Disorder Elixirs, antioxidation Elixirs, an acidify. Japan Patent .JP2010090076.
[4] Yang Li, Liu Yana. the application [J] of enzyme process in Chinese medicine extraction preparation. Chinese herbal medicine, 2001,24(1): 71-73
[5] Zhang Jian, Lin Tinglong, Qin Ying, etc. beta amylase progress [J]. China brewages, 2009,4:5-8
[6] Yu Haixia. the research [D] of Flos Robiniae Pseudoacaciae and acacia honey finger printing: [Master's thesis]. Jilin, Jilin Agriculture University, 2009,6.
Brief description of the drawings
Fig. 1: the HPLC-UV of arginine derivative AF, AFG figure (1, AF in Radix Ginseng of the present invention; 2, AFG);
Fig. 2: the HPLC-UV of arginine derivative AF, AFG figure (1, AF in enzymolysis Radix Ginseng of the present invention; 2, AFG);
Fig. 3: total saponins HPLC-UV figure (1, Rg1 in Radix Ginseng of the present invention; 2, Re; 3, Rf; 4, Rg2; 5, Rb1; 6, Rc; 7, Rb2; 8, Rb3; 9, Rd);
Fig. 4: total saponins HPLC-UV figure (1, Rg1 in enzymolysis Radix Ginseng of the present invention; 2, Re; 3, Rf; 4, Rg2; 5, Rb1; 6, Rc; 7, Rb2; 8, Rb3; 9, Rd);
Fig. 5: Radix Ginseng of the present invention and enzymolysis Radix Ginseng are at the absorbance of UVA, UVB, UVC;
Detailed description of the invention
By following examples, the present invention is further described for example, and do not limit the present invention in any way, any change that those of ordinary skill in the art made for the present invention easily realize or change do not deviating under the prerequisite of technical solution of the present invention, within all will fall into claim scope of the present invention.
the processing technology of enzymolysis Radix Ginseng
Get Radix Ginseng powder (80 order) 5g, regulate solid-liquid ratio 1:10, pH5, adds cellulase by 500:1, enzymolysis 2h, after 100 DEG C of deactivations, regulate pH5.2, by 45g:1 μ L(50 ten thousand u/mL) add beta amylase, high-temperature inactivation after enzymolysis 24h, by 70 DEG C of oven dry of the Radix Ginseng powder after enzymolysis, finally obtain enzymolysis Radix Ginseng 5.01g.
the processing technology of enzymolysis Radix Ginseng
Get Radix Ginseng powder (80 order) 10g, regulate solid-liquid ratio 1:15, pH5.2, adds cellulase by 400:1, enzymolysis 2.5h, after 100 DEG C of deactivations, regulate pH5.2, by 40g:1 μ L(50 ten thousand u/mL) add beta amylase, high-temperature inactivation after enzymolysis 30h, by 70 DEG C of oven dry of the Radix Ginseng powder after enzymolysis, finally obtain enzymolysis Radix Ginseng 10.00g.
embodiment 3
the processing technology of enzymolysis Radix Ginseng
Get Radix Ginseng powder (80 order) 15g, regulate solid-liquid ratio 1:20, pH5.5, adds cellulase by 450:1, enzymolysis 3h, after 100 DEG C of deactivations, regulate pH5.5, by 50g:1 μ L(50 ten thousand u/mL) add beta amylase, high-temperature inactivation after enzymolysis 36h, by 70 DEG C of oven dry of the Radix Ginseng powder after enzymolysis, finally obtain enzymolysis Radix Ginseng 14.85g.
enzymolysis ginseng vanishing cream's preparation
Wetting agent propylene glycol 5.0g, triethanolamine 0.4g are added in Purified Water 70 DEG C of heating for dissolving, by oil content stearic acid 8.0g, after stearyl alcohol 4.0g, butyl stearate 6.0g dissolve, add surfactant glyceryl monostearate 2.0g, antiseptic, the each 0.05g enzymolysis of antioxidant vitamin E Radix Ginseng extract 0.5g, the deionized water amount of supplying, to 100g, is enzymolysis ginseng vanishing cream.
the preparation of enzymolysis ginseng nutrient cream
By wetting agent 1.3-butanediol 6.0g, PEG1500 4.0g joins in the deionized water of 10g and dissolves, adjust 70 DEG C of heating, at oil content stearyl alcohol 5.0g, stearic acid 2.0g, hydrogenation lanoline 4.0g, after 2-octyldodecanol 10.g dissolves, add surfactant POE(25) 1.0g, spermaceti alcohol ether 3.0g, glyceryl monostearate 2.0g, antiseptic ethyl hydroxybenzoate 0.1g, antioxidant vitamin C 0.5g, the enzymolysis Radix Ginseng extract 1g that the present invention is prepared, surplus adds water and supplies 100g, at 70 DEG C, heating in water bath dissolves and mixes, this is joined in water, use homogenizing blender by emulsified particle homogeneous, degassed, filter, cooling.Obtain enzymolysis ginseng nutrient cream.
embodiment 6
the preparation of enzymolysis Radix Ginseng eye cream
By wetting agent dipropylene glycol 5.0g, glycerol 5.0g, triethanolamine 1.0g joins in the deionized water of amount of 10g, 70 DEG C of adjustment, oil content comprises spermol 5.0g, stearic acid 3.0g, vaseline 5.0g, Squalene 10.0g, after glycerol three-2-ethylhexanoate 7.0g heating for dissolving, adds surfactant propylene glycol monostearate 3.0g, POE(20) 2g, spermaceti alcohol ether 3.0g, antiseptic ethyl hydroxybenzoate 0.1g, the enzymolysis Radix Ginseng extract 1g that the present invention is prepared, adds water and supplies 100g.This is added in above-mentioned water and carries out pre-emulsification, re-use homogenizing blender by emulsified particle homogeneous, degassed, filter, cooling.Obtain enzymolysis Radix Ginseng eye cream.
the preparation of enzymolysis Radix Ginseng emulsion
Dipropylene glycol 5.0g, PEG1500 3.0g, triethanolamine 1.0g are joined in Purified Water 75g, adjust 70 DEG C of heating.By stearic acid 2.0g, vaseline 4.0g, dimethyl polysiloxane 2.0g, after glycerol three-2-ethylhexanoate 2.0g dissolves at 70 DEG C, by sorbitan monooleate 2.0g, the enzymolysis Radix Ginseng extract 0.1g that the present invention is prepared, Aloe extract 1g, antiseptic 0.05g and spice 0.001g join in oil content, at 70 DEG C, heating is adjusted, oil phase is joined in water, add water and supply 100g, carry out pre-emulsification.By after emulsifying ion homogeneous, degassed in homogenizing blender, filter coolingly, obtain enzymolysis Radix Ginseng emulsion.
embodiment 8
the preparation of enzymolysis Radix Ginseng sunscreen cream
Octyl methoxycinnamate 5.0g, paraffin oil 6g, C12～15 benzoate 2.5g, lanonol E075 2.0g, methyl glucoside fatty acid ester 0.6g, hexadecanol～octadecanol 4.0g, ethoxylation methyl glucoside fatty acid ester 1.2g, deionized water 63.0g, propylene glycol 5.0g, the enzymolysis Radix Ginseng extract 0.1g that the present invention is prepared, essence and antiseptic are appropriate.By after emulsifying ion homogeneous, degassed, cooling in homogenizing blender, obtain enzymolysis Radix Ginseng sunscreen cream.
the preparation of enzymolysis Radix Ginseng coffee
By enzymolysis Radix Ginseng (crossing 200 mesh sieves) powder 0.1kg, instant coffee powder 1.5kg, white sugar 4.2kg, non-dairy creamer 4.2kg, mix, and obtains enzymolysis Radix Ginseng coffee
the preparation of enzymolysis ginseng candies
By enzymolysis Radix Ginseng (crossing 200 mesh sieves) powder 1kg, Saccharum Sinensis Roxb. 46kg, sodium citrate 0.4kg, glucose syrup (DE～40%) 30kg, water 30kg, pectin 1.50kg, citric acid 0.72kg, essence pigment is appropriate, in homogenizing blender, stirs, and obtains enzymolysis ginseng candies.
embodiment 11
the preparation of enzymolysis Radix Ginseng tea beverage
Black tea 5kg, citric acid 0.5kg, caramel color 0.05kg, vitamin C 0.5kg, high fructose syrup 60kg, black tea spices 0.5kg, the enzymolysis Radix Ginseng 0.5kg that the present invention is prepared, adds pure water to 1000kg, obtains enzymolysis Radix Ginseng tea beverage.
Claims (4)
1. an enzymolysis Radix Ginseng preparation technology who utilizes cellulase to combine with amylase, comprises the following steps:
1) get a certain amount of Radix Ginseng powder (80 order), regulate solid-liquid ratio 1:10～1:20, pH5～5.5, add cellulase by 400:1～500:1, enzymolysis 2～3h, 100 DEG C of deactivations.
2) regulate pH5.2～5.8, by 40g～50g:1 μ L(50 ten thousand u/ml) beta amylase, high-temperature inactivation after enzymolysis 24～36h, by 70 DEG C of oven dry of the Radix Ginseng powder after enzymolysis, to obtain final product.
2. the enzymolysis Radix Ginseng that prepared by method claimed in claim 1 has hepatoprotective effect, can be applicable to liver-protective health food.
3. the enzymolysis Radix Ginseng that prepared by method claimed in claim 1 has uvioresistant radiation protection, can be used for sun care preparations.
4. the enzymolysis Radix Ginseng that prepared by method claimed in claim 1 has stronger antifatigue effect, can be used as health food application.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104605360A (en) * | 2015-02-16 | 2015-05-13 | 大连非得生物产业有限公司 | Functional food capable of improving physical immunity and preparation method thereof |
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| CN106074958A (en) * | 2016-06-20 | 2016-11-09 | 卞佳林 | A kind of have the Chinese medicine composition of sun-screening function, facial cream and preparation method |
| CN106912760A (en) * | 2016-09-30 | 2017-07-04 | 郑毅男 | A kind of preparation method of Jinseng health-care drink |
| CN110123821A (en) * | 2019-05-30 | 2019-08-16 | 大连民族大学 | A kind of medicinal usage of arginine disaccharide glycosides |
| CN110123821B (en) * | 2019-05-30 | 2021-05-14 | 大连民族大学 | Application of argininyl-fructosyl-L-Arginine (ADG) diglycoside as active ingredient in preparation of medicine for treating or preventing acute liver failure diseases |
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