CN103891620A - Taxus chinensis var mairei cultivation method - Google Patents
Taxus chinensis var mairei cultivation method Download PDFInfo
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- CN103891620A CN103891620A CN201410168400.3A CN201410168400A CN103891620A CN 103891620 A CN103891620 A CN 103891620A CN 201410168400 A CN201410168400 A CN 201410168400A CN 103891620 A CN103891620 A CN 103891620A
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Abstract
The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.
Description
Technical field
The present invention relates to plant cultivation field, specifically a kind of breeding method of southern enqlish yew.
Background technology
Southern enqlish yew is aiphyllium, and sprig becomes yellow green or light red brown, leaf bar shaped, dioecism, seed oblateness autumn.Seed is used for extracting oil, and also can be used as medicine.Belong to shallow rooted plant, main root is not obvious, and lateral root prosperity is the endangered natural rare plants for anticancer of generally acknowledging in the world, is through the ancient seeds that carry over of quaternary glacier, the on earth history of existing 2,500,000 years.Under natural environment, the problem that southern enqlish yew ubiquity growth rate is slow, growth cycle is long, regeneration capacity is poor, survival rate is low, therefore for a long time, does not also form the southern enqlish yew raw material forest cultivation base that large rule are touched in world wide.
Summary of the invention
The present invention is directed to the problems referred to above, provide a kind of simple to operate, the cultivation time is short, with low cost, the breeding method of the southern enqlish yew that survival rate is high.
A breeding method for southern enqlish yew, is characterized in that, first southern enqlish yew is organized to cultivation, obtains test-tube plantlet, then test-tube plantlet is carried out to cuttage after hardening, and concrete incubation step is as follows:
One, the tissue of southern enqlish yew is cultivated
1. gather southern enqlish yew current-year branch, after repeatedly rinsing, carry out water planting, tender shoots length grows to 1-2cm scissors and cuts and put into beaker, and beaker mouth covers with gauze, and Crystal drops were dripping down waters 2 ± 0.2 hours; Take out tender shoots to immerse concentration be 75% in alcohol, within 30 seconds, take out afterwards, with aseptic water washing 3 times, then to put into concentration be that 4% 84 thimerosals are processed 7 minutes, by sterile water wash 3-5 times; Sterile working after suck dry moisture, blade on tender shoots is wiped out, and the stem of tender shoots is cut into length is 1 ± 0.2 centimetre, stem section with internode, stem section is inoculated in rich selenium MS medium, is initial incubation 12 days in 25 DEG C, illumination every day 16 hours, the intensity of illumination environment that is 500-750lux in temperature;
2. the petiole of the aseptic seedling leaf of initial incubation is cut into 0.5 centimetre of 0.5 cm x, is transferred in every liter of MS medium that contains 1.0 milligrams of 6-benzyl aminopurines and 1.0 milligrams of methyl α-naphthyl acetates 26 DEG C and cultivates 45 days; Then callus piece is cut under aseptic condition to 0.2 centimetre of 0.2 cm x 0.2 cm x, be transferred to every liter containing in the rich selenium MS medium of 0.5 milligram of methyl α-naphthyl acetate and 1.0 milligrams of 6-benzyl aminopurines 25 DEG C cultivate 30 days, put out new shoots;
3. young shoot step being grown in is 2. transplanted in every liter of 1/2MS medium that contains 1.0 milligrams of methyl α-naphthyl acetates and 10 milligrams of paclobutrazols and is cultivated 20 days, obtains southern enqlish yew test-tube plantlet, and through hardening, the tissue that completes southern enqlish yew is cultivated;
Two, the cuttage of southern enqlish yew
1. build greenhouse cuttage booth: select to be embosomed in hills, booth is built in the area that physical features is lower, and booth has around dug drain ditch and accomplished can arrange and can fill with, with steel pipe build that both sides are high 2 meters, the dome-shaped booth of high 3.5 meters of centre, canopy wide 8 meters, grow 30 meters; Canopy outside is repaiied one dark 1.5 meters, wide 3 meters, and sprinkling irrigation system is installed in the pond of long 5 meters in canopy with pvc pipe, and sprinkling irrigation is drawn water in pond with water pump;
2. whole work: be the native ridge of 1.5 meters by the multiple width of manual amendment in canopy, leave irrigation canals and ditches wide 30 centimetres, dark 10 centimetres between each native ridge;
3. prepare Nutrition Soil: the mass ratio of the formula Fu Zhi Tu ︰ Song Lin ︰ Huang Tu ︰ You Ji Fei ︰ selenium of southern enqlish yew Nutrition Soil is 6-9:1-4:8-10:0.5-1.5:0.0005-0.001;
4. dress cup: ready Nutrition Soil is packed in the nutrition cup of 10 × 8 centimetres, the Nutrition Soil packing into is lower than 1 centimetre of rim of a cup; To install neat being placed on cut-and-dried native ridge of native nutrition cup, put tightly, ajust;
5. transplant: the seedling inoculation that completes hardening is put into nutrition cup and cultivate, after branch reaches semi-lignified, the branch that clip is 8 centimetres is used for cuttage;
6. cuttage: 3. and 4. again prepare Nutrition Soil according to step, first water before cuttage, cuttage branch skewer is entered in the soil in nutrition cup, the cuttage degree of depth is 5 centimetres, every glass of cuttage 3-5 root;
7. cover: after cuttage, adopt plastic foil to carry out capping native ridge, capping is highly 30-50 centimetre, middle with supporting bamboo side;
8. daily management: carry out daily management according to southern enqlish yew conventional daily management method.
Described rich selenium MS medium for adding 100,000 in traditional MS medium/ inorganic selenium element be prepared from.
The optimization formula of described southern enqlish yew Nutrition Soil is: Fu Zhi Tu ︰ Song Lin ︰ Huang Tu ︰ You Ji Fei ︰ selenium mass ratio is 7:3:10:1.0:0.0008.
Compared with prior art, beneficial effect of the present invention is: 1, in tissue culture medium (TCM) and Nutrition Soil, add selenium element, can effectively promote the growth of southern enqlish yew in tissue culture procedures and cuttage process.2, method for tissue culture of the present invention is simple to operate, technical scheme maturation, and the cultivation time is short, with low cost, and survival rate is high, and rooting rate is high, and can in the short time, realize bottle outlet and transplant.3, method for tissue culture of the present invention can keep the merit of former kind, breeds with agricultural modernization and haves laid a good foundation for southern enqlish yew.4, cottage method operating procedure of the present invention is simple, survival rate is high, is all suitable at southern china wide geographic area, has good effect.
Embodiment
Further set forth breeding method of the present invention below in conjunction with embodiment.
Embodiment
One, the tissue of southern enqlish yew is cultivated
1. gather southern enqlish yew current-year branch, after repeatedly rinsing, carry out water planting, tender shoots length grows to 1-2cm scissors and cuts and put into beaker, and beaker mouth covers with gauze, and Crystal drops were dripping down waters 2.2 hours; Take out tender shoots to immerse concentration be 75% in alcohol, within 30 seconds, take out afterwards, with aseptic water washing 3 times, then to put into concentration be that 4% 84 thimerosals are processed 7 minutes, by sterile water wash 5 times; Sterile working after suck dry moisture, blade on tender shoots is wiped out, and the stem of tender shoots is cut into length is 1.2 centimetres, stem section with internode, stem section is inoculated in rich selenium MS medium, be initial incubation 12 days in 25 DEG C, illumination every day 16 hours, the intensity of illumination environment that is 750lux in temperature, rich selenium MS medium for adding 100,000 in traditional MS medium/ inorganic selenium element be prepared from;
2. the petiole of the aseptic seedling leaf of initial incubation is cut into 0.5 centimetre of 0.5 cm x, is transferred in every liter of MS medium that contains 1.0 milligrams of 6-benzyl aminopurines and 1.0 milligrams of methyl α-naphthyl acetates 26 DEG C and cultivates 45 days; Then callus piece is cut under aseptic condition to 0.2 centimetre of 0.2 cm x 0.2 cm x, be transferred to every liter containing in the rich selenium MS medium of 0.5 milligram of methyl α-naphthyl acetate and 1.0 milligrams of 6-benzyl aminopurines 25 DEG C cultivate 30 days, put out new shoots;
3. young shoot step being grown in is 2. transplanted in every liter of 1/2MS medium that contains 1.0 milligrams of methyl α-naphthyl acetates and 10 milligrams of paclobutrazols and is cultivated 20 days, obtains southern enqlish yew test-tube plantlet, and through hardening, the tissue that completes southern enqlish yew is cultivated;
Two, the cuttage of southern enqlish yew
1. build greenhouse cuttage booth: select to be embosomed in hills, booth is built in the area that physical features is lower, and booth has around dug drain ditch and accomplished can arrange and can fill with, with steel pipe build that both sides are high 2 meters, the dome-shaped booth of high 3.5 meters of centre, canopy wide 8 meters, grow 30 meters; Canopy outside is repaiied one dark 1.5 meters, wide 3 meters, and sprinkling irrigation system is installed in the pond of long 5 meters in canopy with pvc pipe, and sprinkling irrigation is drawn water in pond with water pump;
2. whole work: be the native ridge of 1.5 meters by the multiple width of manual amendment in canopy, leave irrigation canals and ditches wide 30 centimetres, dark 10 centimetres between each native ridge, rain and be used for draining flooded fields, can be used at ordinary times walking, the whole broken leveling of native ridge soil block;
3. prepare Nutrition Soil: the mass ratio of the formula Fu Zhi Tu ︰ Song Lin ︰ Huang Tu ︰ You Ji Fei ︰ selenium of southern enqlish yew Nutrition Soil is 7:3:10:1.0:0.0008;
4. dress cup: ready Nutrition Soil is packed in the nutrition cup of 10 × 8 centimetres, the Nutrition Soil packing into is lower than 1 centimetre of rim of a cup; To install neat being placed on cut-and-dried native ridge of native nutrition cup, put tightly, ajust;
5. transplant: the seedling inoculation that completes hardening is put into nutrition cup and cultivate, after branch reaches semi-lignified, the branch that clip is 8 centimetres is used for cuttage;
6. cuttage: 3. and 4. again prepare Nutrition Soil according to step, first water before cuttage, cuttage branch skewer is entered in the soil in nutrition cup, the cuttage degree of depth is 5 centimetres, 5 of every glass of cuttages;
7. cover: after cuttage, adopt plastic foil to carry out capping native ridge, capping is highly 50 centimetres, middle with supporting bamboo side;
8. daily management: carry out daily management according to southern enqlish yew conventional daily management method.
Because selenium is the indispensable element of animal and plant growth, it is the part of glutathione peroxidase mutase and P-albumen; If do not add selenium element, just easily cause lacking this element, it is poor that group training seedling grows up to rear diseases and insect pests resistance, and after implanting in soil, survival rate only has 60%-70%; Breeding method of the present invention adds selenium element in MS medium, and group training seedling stalwartness, the diseases and insect pests resistance of turning out are strong, implant after soil, and survival rate is more than 90%; In the Nutrition Soil of rich selenium, carry out cuttage survival rate and reach more than 98%, greatly improved the survival rate of cuttage.
Claims (3)
1. a breeding method for southern enqlish yew, is characterized in that, first southern enqlish yew is organized to cultivation, obtains test-tube plantlet, then test-tube plantlet is carried out to cuttage after hardening, and concrete incubation step is as follows:
One, the tissue of southern enqlish yew is cultivated
1. gather southern enqlish yew current-year branch, after repeatedly rinsing, carry out water planting, tender shoots length grows to 1-2cm scissors and cuts and put into beaker, and beaker mouth covers with gauze, and Crystal drops were dripping down waters 2 ± 0.2 hours; Take out tender shoots to immerse concentration be 75% in alcohol, within 30 seconds, take out afterwards, with aseptic water washing 3 times, then to put into concentration be that 4% 84 thimerosals are processed 7 minutes, by sterile water wash 3-5 times; Sterile working after suck dry moisture, blade on tender shoots is wiped out, and the stem of tender shoots is cut into length is 1 ± 0.2 centimetre, stem section with internode, stem section is inoculated in rich selenium MS medium, is initial incubation 12 days in 25 DEG C, illumination every day 16 hours, the intensity of illumination environment that is 500-750lux in temperature;
2. the petiole of the aseptic seedling leaf of initial incubation is cut into 0.5 centimetre of 0.5 cm x, is transferred in every liter of MS medium that contains 1.0 milligrams of 6-benzyl aminopurines and 1.0 milligrams of methyl α-naphthyl acetates 26 DEG C and cultivates 45 days; Then callus piece is cut under aseptic condition to 0.2 centimetre of 0.2 cm x 0.2 cm x, be transferred to every liter containing in the rich selenium MS medium of 0.5 milligram of methyl α-naphthyl acetate and 1.0 milligrams of 6-benzyl aminopurines 25 DEG C cultivate 30 days, put out new shoots;
3. young shoot step being grown in is 2. transplanted in every liter of 1/2MS medium that contains 1.0 milligrams of methyl α-naphthyl acetates and 10 milligrams of paclobutrazols and is cultivated 20 days, obtains southern enqlish yew test-tube plantlet, and through hardening, the tissue that completes southern enqlish yew is cultivated;
Two, the cuttage of southern enqlish yew
1. build greenhouse cuttage booth: select to be embosomed in hills, booth is built in the area that physical features is lower, and booth has around dug drain ditch and accomplished can arrange and can fill with, with steel pipe build that both sides are high 2 meters, the dome-shaped booth of high 3.5 meters of centre, canopy wide 8 meters, grow 30 meters; Canopy outside is repaiied one dark 1.5 meters, wide 3 meters, and sprinkling irrigation system is installed in the pond of long 5 meters in canopy with pvc pipe, and sprinkling irrigation is drawn water in pond with water pump;
2. whole work: be the native ridge of 1.5 meters by the multiple width of manual amendment in canopy, leave irrigation canals and ditches wide 30 centimetres, dark 10 centimetres between each native ridge;
3. prepare Nutrition Soil: the mass ratio of the formula Fu Zhi Tu ︰ Song Lin ︰ Huang Tu ︰ You Ji Fei ︰ selenium of southern enqlish yew Nutrition Soil is 6-9:1-4:8-10:0.5-1.5:0.0005-0.001;
4. dress cup: ready Nutrition Soil is packed in the nutrition cup of 10 × 8 centimetres, the Nutrition Soil packing into is lower than 1 centimetre of rim of a cup; To install neat being placed on cut-and-dried native ridge of native nutrition cup, put tightly, ajust;
5. transplant: the seedling inoculation that completes hardening is put into nutrition cup and cultivate, after branch reaches semi-lignified, the branch that clip is 8 centimetres is used for cuttage;
6. cuttage: 3. and 4. again prepare Nutrition Soil according to step, first water before cuttage, cuttage branch skewer is entered in the soil in nutrition cup, the cuttage degree of depth is 5 centimetres, every glass of cuttage 3-5 root;
7. cover: after cuttage, adopt plastic foil to carry out capping native ridge, capping is highly 30-50 centimetre, middle with supporting bamboo side;
8. daily management: carry out daily management according to southern enqlish yew conventional daily management method.
2. the breeding method of a kind of southern enqlish yew according to claim 1, is characterized in that, described rich selenium MS medium for adding 100,000 in traditional MS medium/ inorganic selenium element be prepared from.
3. the breeding method of a kind of southern enqlish yew according to claim 1, is characterized in that, the optimization formula of described southern enqlish yew Nutrition Soil is: Fu Zhi Tu ︰ Song Lin ︰ Huang Tu ︰ You Ji Fei ︰ selenium mass ratio is 7:3:10:1.0:0.0008.
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| CN201410168400.3A CN103891620A (en) | 2014-04-25 | 2014-04-25 | Taxus chinensis var mairei cultivation method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186160A (en) * | 2014-08-28 | 2014-12-10 | 江苏红豆杉生物科技股份有限公司 | Indoor care method for taxus chinensis in summer |
| CN104186163A (en) * | 2014-08-28 | 2014-12-10 | 郎溪县凌笪乡永辰茶叶种植家庭农场 | Seeding and cultivating method for nandina domestica |
| CN105075583A (en) * | 2015-07-25 | 2015-11-25 | 安徽博发文化生态园有限公司 | Planting method of selenium-rich kumquats |
| CN105309141A (en) * | 2014-07-14 | 2016-02-10 | 卢鹏 | Taxus chinensis propagating method |
| CN105393880A (en) * | 2015-11-27 | 2016-03-16 | 广德经济开发区绿源生态家庭农场 | Chinese yew high survival rate cutting seedling method |
| CN106489481A (en) * | 2016-10-19 | 2017-03-15 | 贵州省亚热带作物研究所 | Promote Radix Puerariae cutting lignifying method |
| CN107173223A (en) * | 2017-05-22 | 2017-09-19 | 金英子 | A kind of cultivation of plants method |
| CN109601389A (en) * | 2019-01-30 | 2019-04-12 | 徐英奎 | It is mixed to hand over the asexual efficient tissue culture technique of procreation S7 Chinese yew |
| CN111279963A (en) * | 2020-02-22 | 2020-06-16 | 中国林业科学研究院亚热带林业实验中心 | Method for promoting flowering and fruiting of female taxus chinensis var mairei |
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2014
- 2014-04-25 CN CN201410168400.3A patent/CN103891620A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309141A (en) * | 2014-07-14 | 2016-02-10 | 卢鹏 | Taxus chinensis propagating method |
| CN104186160A (en) * | 2014-08-28 | 2014-12-10 | 江苏红豆杉生物科技股份有限公司 | Indoor care method for taxus chinensis in summer |
| CN104186163A (en) * | 2014-08-28 | 2014-12-10 | 郎溪县凌笪乡永辰茶叶种植家庭农场 | Seeding and cultivating method for nandina domestica |
| CN104186160B (en) * | 2014-08-28 | 2016-07-06 | 江苏红豆杉生物科技股份有限公司 | Taxus mairei indoor in summer maintenance process |
| CN105075583A (en) * | 2015-07-25 | 2015-11-25 | 安徽博发文化生态园有限公司 | Planting method of selenium-rich kumquats |
| CN105393880A (en) * | 2015-11-27 | 2016-03-16 | 广德经济开发区绿源生态家庭农场 | Chinese yew high survival rate cutting seedling method |
| CN106489481A (en) * | 2016-10-19 | 2017-03-15 | 贵州省亚热带作物研究所 | Promote Radix Puerariae cutting lignifying method |
| CN109197547A (en) * | 2017-05-22 | 2019-01-15 | 金英子 | A kind of plant cultivation method based on solar LED (Light Emitting Diode) lamp and intelligent Sprinkling Irrigation |
| CN107173223A (en) * | 2017-05-22 | 2017-09-19 | 金英子 | A kind of cultivation of plants method |
| CN109197548A (en) * | 2017-05-22 | 2019-01-15 | 金英子 | A kind of plant cultivation method based on solar LED (Light Emitting Diode) lamp and intelligent Sprinkling Irrigation |
| CN109197549A (en) * | 2017-05-22 | 2019-01-15 | 金英子 | A kind of plant cultivation method based on solar LED (Light Emitting Diode) lamp and intelligent Sprinkling Irrigation |
| CN109197546A (en) * | 2017-05-22 | 2019-01-15 | 金英子 | A kind of plant cultivation method based on solar LED (Light Emitting Diode) lamp and intelligent Sprinkling Irrigation |
| CN109302976A (en) * | 2017-05-22 | 2019-02-05 | 金英子 | A kind of plant cultivation method based on solar LED (Light Emitting Diode) lamp and intelligent Sprinkling Irrigation |
| CN109601389A (en) * | 2019-01-30 | 2019-04-12 | 徐英奎 | It is mixed to hand over the asexual efficient tissue culture technique of procreation S7 Chinese yew |
| CN111279963A (en) * | 2020-02-22 | 2020-06-16 | 中国林业科学研究院亚热带林业实验中心 | Method for promoting flowering and fruiting of female taxus chinensis var mairei |
| CN111279963B (en) * | 2020-02-22 | 2021-12-28 | 中国林业科学研究院亚热带林业实验中心 | Method for promoting flowering and fruiting of female taxus chinensis var mairei |
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Application publication date: 20140702 |