CN103881921A - Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin - Google Patents
Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin Download PDFInfo
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- CN103881921A CN103881921A CN201210561604.4A CN201210561604A CN103881921A CN 103881921 A CN103881921 A CN 103881921A CN 201210561604 A CN201210561604 A CN 201210561604A CN 103881921 A CN103881921 A CN 103881921A
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- astaxanthin
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Abstract
The invention discloses an improved BBM medium formula for Haematococcus pluvialis which is capable of high yielding of astaxanthin. The formula comprises, by mass, 0.9 to 1.1 parts of NaNO3, 0.9 to 1.1 parts of K2HPO4, 0.9 to 1.1 parts of MgSO4.7H2O, 0.9 to 1.1 parts of CaCl2.2H2O, 0.9 to 1.1 parts of KH2PO4, 0.9 to 1.1 parts of NaCl, 0.9 to 1.1 parts of B1, 0.225 to 0.275 part of vitamin H, 0.225 to 0.275 part of B12 and 0.9 to 1.1 parts of a trace element. According to the invention, NaHCO3, vitamin B12 and Fe3+ are added on the basis of a conventional BBM formula, the growth speed of cultured Haematococcus pluvialis is greatly increased, and astaxanthin yield is greatly improved.
Description
Technical field
The invention belongs to micro-algae productive culture base technical field, be specifically related to the Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin.
Background technology
Haematocoocus Pluvialls is a kind of unicell green alga, and accumulation astaxanthin in a large number is under given conditions the highest biology (10-30mg/g) of occurring in nature accumulation natural astaxanthin ability.Natural astaxanthin has superpower anti-oxidant activity, there is the good reputation of " supper vitamin E ", can be used as fodder additives, natural food colour and biological health-care product, medicine etc., throughput far can not be met the need of market at present, therefore utilizes Haematococcus pluvialis production natural astaxanthin to receive much attention at home and abroad.It is slow that the culture medium prescription ubiquity of existing cultivation Haematocoocus Pluvialls is cultivated the speed of growth of Haematocoocus Pluvialls, the defect that astaxanthin productive rate is low.
Summary of the invention
The above-mentioned defect existing in order to overcome prior art field, the object of the invention is to, a kind of Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin is provided, it is slow that the culture medium prescription ubiquity of solution prior art cultivation Haematocoocus Pluvialls is cultivated the speed of growth of Haematocoocus Pluvialls, the problem that astaxanthin productive rate is low.
The Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin provided by the invention, its components in mass portion proportioning is: NaNO
30.9-1.1 parts, K
2hPO
40.9-1.1 parts, MgSO
47H
20.9-1.1 parts of O, CaCl
22H
20.9-1.1 parts of O, KH
2pO
40.9-1.1 parts, 0.9-1.1 parts of NaCl, 0.9-1.1 parts of B1,0.225-0.275 part of vitamin H, 0.225-0.275 part of B12,0.9-1.1 parts of trace elements; Described trace element comprises Na
2eDTA, MnCl
24H
2o, ZnCl
27H
2o, Na
2moO
42H
2o, FeCl
36 H
2o, CoCl
26H
2o.
The Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin provided by the invention, its beneficial effect is, on original BBM formula basis, adds NaHCO again
3, vitamin B12 and Fe3+, the speed of growth of cultivating Haematocoocus Pluvialls improves greatly, astaxanthin productive rate also improves greatly.
Embodiment
Below in conjunction with an embodiment, the Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin provided by the invention is described in detail.
Embodiment
The Haematocoocus Pluvialls BBM improved culture medium formula of the high-yield astaxanthin of the present embodiment, NaNO
31 part, K
2hPO
41 part, MgSO
47H
21 part of O, CaCl
22H
21 part of O, KH
2pO
41 part, 1 part of NaCl, 1 part of B1,0.25 part of vitamin H, 0.25 part of B12,1 part of trace element; Described trace element comprises Na
2eDTA, MnCl
24H
2o, ZnCl
27H
2o, Na
2moO
42H
2o, FeCl
36 H
2o, CoCl
26H
2o.Astaxanthin productive rate is 24.50mg/L.
Claims (2)
1. the Haematocoocus Pluvialls BBM improved culture medium of high-yield astaxanthin formula, is characterized in that: its components in mass portion proportioning is: NaNO
30.9-1.1 parts, K
2hPO
40.9-1.1 parts, MgSO
47H
20.9-1.1 parts of O, CaCl
22H
20.9-1.1 parts of O, KH
2pO
40.9-1.1 parts, 0.9-1.1 parts of NaCl, 0.9-1.1 parts of B1,0.225-0.275 part of vitamin H, 0.225-0.275 part of B12,0.9-1.1 parts of trace elements.
2. the Haematocoocus Pluvialls BBM improved culture medium of high-yield astaxanthin according to claim 1 formula, is characterized in that:
Described trace element comprises Na
2eDTA, MnCl
24H
2o, ZnCl
27H
2o, Na
2moO
42H
2o, FeCl
36 H
2o, CoCl
26H
2o.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201210561604.4A CN103881921A (en) | 2012-12-22 | 2012-12-22 | Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin |
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CN201210561604.4A CN103881921A (en) | 2012-12-22 | 2012-12-22 | Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin |
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Publication Number | Publication Date |
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CN103881921A true CN103881921A (en) | 2014-06-25 |
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CN201210561604.4A Pending CN103881921A (en) | 2012-12-22 | 2012-12-22 | Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104762211A (en) * | 2015-05-04 | 2015-07-08 | 厦门大学 | Wall-breaking method for haematococcus pluvialis |
CN105420332A (en) * | 2015-12-10 | 2016-03-23 | 天津科技大学 | Method for achieving high yield of astaxanthin through haematococcus pluvialis |
CN105567625A (en) * | 2016-02-26 | 2016-05-11 | 中国水产科学研究院长江水产研究所 | Haematococcus pluvialis induction medium |
CN107460225A (en) * | 2017-10-11 | 2017-12-12 | 广西南宁秀珀生物科技有限公司 | The method of continuous culture patter of rain haematococcus accumulation astaxanthin |
CN107502551A (en) * | 2017-10-11 | 2017-12-22 | 广西南宁秀珀生物科技有限公司 | Utilize the method for bioreactor culture patter of rain haematococcus |
CN107502646A (en) * | 2017-10-11 | 2017-12-22 | 广西南宁秀珀生物科技有限公司 | Utilize the method for patter of rain haematococcus efficient accumulation astaxanthin |
CN107523503A (en) * | 2017-10-11 | 2017-12-29 | 广西南宁秀珀生物科技有限公司 | The cultural method of patter of rain haematococcus |
-
2012
- 2012-12-22 CN CN201210561604.4A patent/CN103881921A/en active Pending
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104762211A (en) * | 2015-05-04 | 2015-07-08 | 厦门大学 | Wall-breaking method for haematococcus pluvialis |
CN105420332A (en) * | 2015-12-10 | 2016-03-23 | 天津科技大学 | Method for achieving high yield of astaxanthin through haematococcus pluvialis |
CN105567625A (en) * | 2016-02-26 | 2016-05-11 | 中国水产科学研究院长江水产研究所 | Haematococcus pluvialis induction medium |
CN107460225A (en) * | 2017-10-11 | 2017-12-12 | 广西南宁秀珀生物科技有限公司 | The method of continuous culture patter of rain haematococcus accumulation astaxanthin |
CN107502551A (en) * | 2017-10-11 | 2017-12-22 | 广西南宁秀珀生物科技有限公司 | Utilize the method for bioreactor culture patter of rain haematococcus |
CN107502646A (en) * | 2017-10-11 | 2017-12-22 | 广西南宁秀珀生物科技有限公司 | Utilize the method for patter of rain haematococcus efficient accumulation astaxanthin |
CN107523503A (en) * | 2017-10-11 | 2017-12-29 | 广西南宁秀珀生物科技有限公司 | The cultural method of patter of rain haematococcus |
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Application publication date: 20140625 |