CN103880538A - Culture medium for morchella and preparation method thereof - Google Patents

Culture medium for morchella and preparation method thereof Download PDF

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Publication number
CN103880538A
CN103880538A CN201410115072.0A CN201410115072A CN103880538A CN 103880538 A CN103880538 A CN 103880538A CN 201410115072 A CN201410115072 A CN 201410115072A CN 103880538 A CN103880538 A CN 103880538A
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China
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morel
preparation
culture base
culture medium
fermentation
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CN201410115072.0A
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Inventor
葛静波
陈春化
王浩东
沈业庭
季林章
孙晓亮
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SIYANG COUNTY INSTITUTE OF AGRICULTURAL SCIENCES
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SIYANG COUNTY INSTITUTE OF AGRICULTURAL SCIENCES
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Abstract

The invention provides a culture medium for morchella and a preparation method thereof and relates to the technical field of edible mushroom cultivation, wherein the culture medium is composed of the following components: peat, soybean curb residue, shaddock peel, tomato seedling, plant ash, monopotassium phosphate, gypsum powder, quick lime and a regulator; the preparation method of the culture medium comprises the steps of (1) material preparation, (2) crushing, (3) mixing, (4) fermentation and (5) packaging. The culture medium for the morchella is added with the peat and a plurality of waste materials on the basis of the traditional culture medium, and thus is capable of meeting the nutrition requirements of the growth of the morchella and also advantageous for hypha production, and moreover, the raw materials are wide in source and the cost is low. The preparation method of the culture medium for the morchella is scientific and rational, and consequently, the nutrition values of various materials can be utilized more effectively, fruiting can be advanced, the fruiting period can be prolonged, and the nutrition value of the morchella is also increased.

Description

Culture base-material of a kind of morel and preparation method thereof
Technical field
The present invention relates to the preparation method of culture base-material He this culture base-material in a kind of fungus growing technique field, particularly a kind of morel.
Background technology
Morel claims again sheep tripe dish, delicious morel, sheep mushroom, morel, is a kind of edible mushrooms and medicinal fungus of preciousness.Morel was found in 1818.Its structure is similar to cup fungi, and it is netted that top is fold, both as individual honeycomb, also as individual sheep tripe, thereby gains the name.Output extra-high-speed in after mountain fire two to three years of morel, therefore the picker of North America can gather morel according to mountain fire.But after fire is controlled, in same area, its growth population can reduce year in year out.
The cultivation history of China morel originates from 1985, start to expand gradually cultivation, but output is extremely unstable the nineties, is that artificial medium is of low quality on the one hand, is the management mode of shortage science on the other hand.To 21 century, the tame technology of morel develops gradually, and output is steadily increasing, has formed the cultivation management method of a set of China's Mode.
Of short duration due to Chinese cultivated history, at present, the cultivation of morel still cannot meet people's demand, and the cultivation of China's Mode is comparatively strict for culture base-material, selects materials and produce into meeting, limit the use of a lot of dead meals, cause cultivation high cost, and the quality of culture base-material unstable, although output is stable, but quality still has much room for improvement, the particularly aminoacids content of morel, apart from larger, nutritive value is lower with the content difference of the morel of field growth.
Summary of the invention
Goal of the invention: for above-mentioned deficiency, overcome the defect of prior art, the object of this invention is to provide the preparation method of culture base-material He this culture base-material of a kind of morel.
A culture base-material for morel, is made up of the component of following mass fraction:
Peat 120-200 part, residue from beans after making 50-80 part, pomelo peel 30-70 part, tomato seedling 50-120 part, plant ash 10-25 part, potassium primary phosphate 10-30 part, urea 12-35 part, terra alba 40-80 part, unslaked lime 60-150 part and conditioning agent 3-6 part, wherein conditioning agent comprises ethychlozate and paclobutrazol, and the mass fraction of ethychlozate is 2-4 part.
As preferably, the water content of described peat is 60%-75%.
As preferably, the mass fraction of unslaked lime is 75-110 part.
A preparation method for the culture base-material of morel, comprises the following steps:
(1) get the raw materials ready: peat 120-200 part, residue from beans after making 50-80 part, pomelo peel 30-70 part, tomato seedling 50-120 part, plant ash 10-25 part, potassium primary phosphate 10-30 part, urea 12-35 part, terra alba 40-80 part, unslaked lime 60-150 part and conditioning agent 3-6 part;
(2) pulverize: the pomelo peel taking and tomato seedling are carried out to pulverization process, and median size is 0.3-1.2mm;
(3) mix: above-mentioned other components except unslaked lime that take are mixed, after mixing, add water while stirring and unslaked lime, and regulate water content and the pH value of mixture, water content 40%-55%, pH value 7.0-7.9;
(4) fermentation: after stirring, be 18-25 ℃ in temperature, the environment bottom fermentation of relative air humidity 75%-90%;
(5) charging: after thing fermentation ends to be mixed, encapsulate with plastics film, the lumphy structure of encapsulation growth 10cm, wide 10cm, thick 3cm, makes.
As preferably, the water content of mix stages mixture is 45%-52%, pH value 7.1-7.6.
As preferably, the fermentation time of fermentation stage is 45-65 day.
Beneficial effect: the culture base-material of morel provided by the invention, on the basis of traditional culture base-material, add peat and multiple waste, not only meet the nutritional needs of morel growth, also utilize mycelia to produce, and material source is extensive, cost is lower.On the other hand, the preparation method of the culture base-material of morel provided by the invention, scientific and reasonable, can more effectively utilize the nutritive value of various materials, and early fruiting, extend the fruiting phase, improve the nutritive value of morel itself.
Embodiment
In order further to understand the present invention, below in conjunction with embodiment, the preferred embodiment of the invention is described, but should be appreciated that these are described is for further illustrating the features and advantages of the present invention, rather than limiting to the claimed invention.
Embodiment 1
A culture base-material for morel, is made up of the component of following mass fraction:
3 parts of 120 parts, peat, 50 parts of residue from beans after makings, 30 parts of pomelo peels, 50 parts of tomato seedlings, 10 parts of plant ash, 10 parts of potassium primary phosphates, 12 parts, urea, 40 parts of terra albas, 75 parts of unslaked limes and conditioning agents, wherein conditioning agent comprises ethychlozate and paclobutrazol, the mass fraction of ethychlozate is 1 part, and paclobutrazol mass fraction is 2 parts.Wherein, the water content of peat is 65%.
A preparation method for the culture base-material of morel, comprises the following steps:
(1) get the raw materials ready: each component that takes above-mentioned mass fraction;
(2) pulverize: the pomelo peel taking and tomato seedling are carried out to pulverization process, and median size is 0.8mm;
(3) mix: above-mentioned other components except unslaked lime that take are mixed, after mixing, add water while stirring and unslaked lime, and regulate water content and the pH value of mixture, water content 50%, pH value 7.2;
(4) fermentation: after stirring, be 23 ℃ in temperature, the environment bottom fermentation of relative air humidity 85%, the fermentation time of fermentation stage is 55;
(5) charging: after thing fermentation ends to be mixed, encapsulate with plastics film, the lumphy structure of encapsulation growth 10cm, wide 10cm, thick 3cm, makes.
Embodiment 2
A culture base-material for morel, is made up of the component of following mass fraction:
6 parts of 200 parts, peat, 80 parts of residue from beans after makings, 70 parts of pomelo peels, 120 parts of tomato seedlings, 25 parts of plant ash, 30 parts of potassium primary phosphates, 35 parts, urea, 80 parts of terra albas, 110 parts of unslaked limes and conditioning agents, wherein conditioning agent comprises ethychlozate and paclobutrazol, the mass fraction of ethychlozate is 3 parts, and the mass fraction of paclobutrazol is 3 parts.Wherein, the water content of peat is 65%.
A preparation method for the culture base-material of morel, comprises the following steps:
(1) get the raw materials ready: each component that takes above-mentioned mass fraction;
(2) pulverize: the pomelo peel taking and tomato seedling are carried out to pulverization process, and median size is 0.8mm;
(3) mix: above-mentioned other components except unslaked lime that take are mixed, after mixing, add water while stirring and unslaked lime, and regulate water content and the pH value of mixture, water content 48%, pH value 7.5;
(4) fermentation: after stirring, be 23 ℃ in temperature, the environment bottom fermentation of relative air humidity 85%, the fermentation time of fermentation stage is 60;
(5) charging: after thing fermentation ends to be mixed, encapsulate with plastics film, the lumphy structure of encapsulation growth 10cm, wide 10cm, thick 3cm, makes.
Embodiment 3
A culture base-material for morel, is made up of the component of following mass fraction:
5 parts of 155 parts, peat, 65 parts of residue from beans after makings, 63 parts of pomelo peels, 82 parts of tomato seedlings, 18 parts of plant ash, 22 parts of potassium primary phosphates, 24 parts, urea, 66 parts of terra albas, 105 parts of unslaked limes and conditioning agents, wherein conditioning agent comprises ethychlozate and paclobutrazol, the mass fraction of ethychlozate is 2 parts, and the mass fraction of paclobutrazol is 3 parts.Wherein, the water content of peat is 65%.
A preparation method for the culture base-material of morel, comprises the following steps:
(1) get the raw materials ready: each component that takes above-mentioned mass fraction;
(2) pulverize: the pomelo peel taking and tomato seedling are carried out to pulverization process, and median size is 0.8mm;
(3) mix: above-mentioned other components except unslaked lime that take are mixed, after mixing, add water while stirring and unslaked lime, and regulate water content and the pH value of mixture, water content 45%, pH value 7.3;
(4) fermentation: after stirring, be 23 ℃ in temperature, the environment bottom fermentation of relative air humidity 85%, the fermentation time of fermentation stage is 58;
(5) charging: after thing fermentation ends to be mixed, encapsulate with plastics film, the lumphy structure of encapsulation growth 10cm, wide 10cm, thick 3cm, makes.
Utilizing the culture base-material of morel cultivation conventional in prior art is control group, makes control group 1.Embodiment 1-3 and control group 1 are carried out to morel experiment in cultivation.In test, indoor temperature, humidity, illumination, ventilation management work and earthing condition are identical, and experiment in cultivation adopts existing indoor de-bag cultivating method, observation fruiting time, fruiting phase and output.The morel afterwards embodiment group and control group being cultivated carries out Analysis of Nutritional test.
Test-results is as follows:
(1) comparative analysis of fruiting time, fruiting phase and output.
The culture base-material control group of embodiment group and prior art, during budding fruiting time, fruiting and output have certain difference.
The fruiting time average of buddingging of embodiment group is done sth. in advance 1.5 days; Stop the fruiting time upper, embodiment group is on average later than control group 1.5 days, and the whole fruiting phase on average extends 3 days; In output, embodiment group on average increases production 8.8% than control group.
(2) difference of the morel main nutrient composition of different culture media material cultivation, result is as shown in table 1:
The impact of table 1 different culture media material cultivation morel on morel main nutrient composition
The difference of the morel of different culture media material cultivation in essential amino acids content, if as shown in table 2:
The morel essential amino acids content (unit: %) of table 2 different culture media material cultivation
As seen from table, the crude protein content of embodiment group and calcium phosphorus potassium content are higher than control group, and crude fat content is a little less than control group; On the content of eight seed amino acids of needed by human, embodiment group has seven kinds of essential amino acids content higher than control group, and a kind of aminoacids content and control group maintain an equal level, and wherein Isoleucine and leucine content are significantly higher than control group.
Therefore the culture base-material that the culture base-material preparation method of morel provided by the invention makes, can make morel shift to an earlier date fruiting compared to prior art, extend the fruiting phase, and on nutritive value, higher than existing culture base-material, not only improve output but also reduced cost, very practical.
To the above-mentioned explanation of the disclosed embodiments, make professional and technical personnel in the field can realize or use the present invention.To be apparent for those skilled in the art to the multiple modification of these embodiment, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.

Claims (6)

1. a culture base-material for morel, is characterized in that being made up of the component of following mass fraction:
Peat 120-200 part, residue from beans after making 50-80 part, pomelo peel 30-70 part, tomato seedling 50-120 part, plant ash 10-25 part, potassium primary phosphate 10-30 part, urea 12-35 part, terra alba 40-80 part, unslaked lime 60-150 part and conditioning agent 3-6 part, wherein conditioning agent comprises ethychlozate and paclobutrazol, and the mass fraction of ethychlozate is 2-4 part.
2. the culture base-material of morel according to claim 1, is characterized in that: the water content of described peat is 60%-75%.
3. the culture base-material of morel according to claim 1, is characterized in that: the mass fraction of unslaked lime is 75-110 part.
4. a preparation method for the culture base-material of morel claimed in claim 1, is characterized in that: comprise the following steps:
(1) get the raw materials ready: peat 120-200 part, residue from beans after making 50-80 part, pomelo peel 30-70 part, tomato seedling 50-120 part, plant ash 10-25 part, potassium primary phosphate 10-30 part, urea 12-35 part, terra alba 40-80 part, unslaked lime 60-150 part and conditioning agent 3-6 part;
(2) pulverize: the pomelo peel taking and tomato seedling are carried out to pulverization process, and median size is 0.3-1.2mm;
(3) mix: above-mentioned other components except unslaked lime that take are mixed, after mixing, add water while stirring and unslaked lime, and regulate water content and the pH value of mixture, water content 40%-55%, pH value 7.0-7.9;
(4) fermentation: after stirring, be 18-25 ℃ in temperature, the environment bottom fermentation of relative air humidity 75%-90%;
(5) charging: after thing fermentation ends to be mixed, encapsulate with plastics film, the lumphy structure of encapsulation growth 10cm, wide 10cm, thick 3cm, makes.
5. the preparation method of the culture base-material of morel according to claim 4, is characterized in that: the water content of mix stages mixture is 45%-52%, pH value 7.1-7.6.
6. the preparation method of the culture base-material of morel according to claim 4, is characterized in that: the fermentation time of fermentation stage is 45-65 day.
CN201410115072.0A 2014-03-26 2014-03-26 Culture medium for morchella and preparation method thereof Pending CN103880538A (en)

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104030851A (en) * 2014-06-30 2014-09-10 桂林丰茂源农业技术开发有限公司 Lentinula edodes culture medium in which shaddock peel is added
CN104163687A (en) * 2014-06-30 2014-11-26 桂林丰茂源农业技术开发有限公司 Edible tree fungus culture medium containing shaddock peel
CN104163684A (en) * 2014-06-30 2014-11-26 桂林丰茂源农业技术开发有限公司 A cordyceps militaris sporophore culture medium comprising shaddock peel
CN104193483A (en) * 2014-07-24 2014-12-10 安徽天都灵芝制品公司 Shaddock peel-containing straw mushroom culture medium and preparation method thereof
CN105218252A (en) * 2015-11-04 2016-01-06 梅庆波 A kind of preparation method of morel cultivation special solid nutritive medium casingmaterial
CN106116743A (en) * 2016-06-15 2016-11-16 铜仁市侗菇菌业发展有限公司 A kind of Morchella esculenta (L.) Pers culture medium for cultivating material
CN107324877A (en) * 2017-06-27 2017-11-07 安徽诺亚农业有限公司 Hickory chick culture matrix prepared by a kind of utilization shaddock ped
CN107567954A (en) * 2017-10-13 2018-01-12 太湖县金江源农业发展有限公司 A kind of cultural method of hickory chick
CN109757301A (en) * 2019-01-23 2019-05-17 广西民族师范学院 A kind of Qinghai-Tibet Platean hickory chick high-yield cultivating material and preparation method thereof
CN112352625A (en) * 2020-11-11 2021-02-12 遵义新欣食用菌专业合作社 Morchella strain stick and processing method thereof

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104030851A (en) * 2014-06-30 2014-09-10 桂林丰茂源农业技术开发有限公司 Lentinula edodes culture medium in which shaddock peel is added
CN104163687A (en) * 2014-06-30 2014-11-26 桂林丰茂源农业技术开发有限公司 Edible tree fungus culture medium containing shaddock peel
CN104163684A (en) * 2014-06-30 2014-11-26 桂林丰茂源农业技术开发有限公司 A cordyceps militaris sporophore culture medium comprising shaddock peel
CN104163684B (en) * 2014-06-30 2016-06-01 桂林丰茂源农业技术开发有限公司 A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin
CN104193483A (en) * 2014-07-24 2014-12-10 安徽天都灵芝制品公司 Shaddock peel-containing straw mushroom culture medium and preparation method thereof
CN105218252A (en) * 2015-11-04 2016-01-06 梅庆波 A kind of preparation method of morel cultivation special solid nutritive medium casingmaterial
CN106116743A (en) * 2016-06-15 2016-11-16 铜仁市侗菇菌业发展有限公司 A kind of Morchella esculenta (L.) Pers culture medium for cultivating material
CN107324877A (en) * 2017-06-27 2017-11-07 安徽诺亚农业有限公司 Hickory chick culture matrix prepared by a kind of utilization shaddock ped
CN107567954A (en) * 2017-10-13 2018-01-12 太湖县金江源农业发展有限公司 A kind of cultural method of hickory chick
CN109757301A (en) * 2019-01-23 2019-05-17 广西民族师范学院 A kind of Qinghai-Tibet Platean hickory chick high-yield cultivating material and preparation method thereof
CN109757301B (en) * 2019-01-23 2024-03-29 红河学院 High-yield cultivation material for morchella in Qinghai-Tibet plateau and preparation method thereof
CN112352625A (en) * 2020-11-11 2021-02-12 遵义新欣食用菌专业合作社 Morchella strain stick and processing method thereof

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Application publication date: 20140625