CN103224422B - Compatibility of hericium erinaceus culture material and manufacturing method of culture material - Google Patents

Abstract

The invention relates to a hericium erinaceus culture material which is characterized in that leftovers in the brewing industry, namely vinegar residue and broad bean hull are taken as main raw materials, and the water content in the culture material is 63%-65%. The invention also relates to a manufacturing method of the culture material. Compared with the existing culture material, the hericium erinaceus culture material has the following advantages: 1) the materials are taken locally, so that the production cost is reduced; 2) the acidity of the vinegar residue raw material is appropriate, the nutrition is rich, and after the vinegar residue is matched with the broad bean hull, the culture material has the advantages of good air permeability, good water retention property, luxuriant growth of mycelia, tidy growth of fruiting bodies, high yield and good quality; and 3) by utilizing the vinegar residue to culture hericium erinaceus, the channels of the culture raw materials are expanded, the utilization rate of resources is improved, and the economic income of a brewing enterprise is simultaneously increased.

Description

The making method of a kind of Hericium erinaceus culture material compatibility and this planting material

Technical field

Content of the present invention comprises Hericium erinaceus culture material compatibility, and the making method of this planting material, belongs to fungus growing technique field.

Background technology

Have the hedgehog hydnum of the title of " mountain delicacy ", nutritious, be famous nourishing body-strengening food, also there is higher pharmaceutical use simultaneously, have very high curative ratio to the tumour of some Digestive tract and inflammation.

Current Hericium erinaceus (Bull. Ex Fr.) Pers. artificial culture materials are mostly cotton seed hulls, hardwood sawdust etc., along with the fast development of mushroom industry, production-scale expansion, Hericium erinaceus (Bull. Ex Fr.) Pers. produces the problem also facing shortage of raw materials and production cost rising, therefore each production unit all attaches great importance to looking for cheap and good-quality alternative materials, screens the cultivation major ingredient being suitable for Hericium erinaceus (Bull. Ex Fr.) Pers. growth with suiting measures to local conditions.

In recent years, because the standard of living of human consumer improves constantly, the vinegar that solid state fermentation is produced is well received by consumers, for meeting the need of market, its output constantly increases, but the consequent is the waste vinegar grain produced in production process also rolls up, in need of immediate treatment.

Vinegar grain is the residue after vinegar made by the raw materials such as corn, jowar, wheat, broad bean, the main tankage making vinegar, it contains crude protein 6%-10%, crude fat 2%-5%, nitrogen-free extract 20%-30%, coarse ash 13%-17%, calcium 0.25%-0.45%, phosphorus 0.16%-0.37%, acid higher (pH is about 4), nutritious; The main component of tankage broad bean skin is Mierocrystalline cellulose, hemicellulose, xylogen, in addition still containing certain mineral substance, protein, VITAMIN etc., and wherein Mierocrystalline cellulose 56.8%, hemicellulose 11.8%, xylogen 8.7%, crude protein 4.64%, structural protein 4.12%.

Hericium erinaceus (Bull. Ex Fr.) Pers. belongs to the acid mushroom of happiness, and vegetative stage is with pH4 optimum, and the sporophore growth stage is with pH4-5 optimum.The nutritive ingredient of broad bean skin, vinegar grain and the nutrient needed for Hericium erinaceus (Bull. Ex Fr.) Pers. very identical, be therefore the comparatively ideal raw material of Hericium erinaceus culture.

What utilize these tankage to be not only an Edible Fungi falls this behave, but also is a circular agriculture and environmental protection cause behave.

Summary of the invention

The present invention has following two aspects:

1, a kind of Hericium erinaceus culture material compatibility

2, the making method of planting material

The technical solution adopted in the present invention is:

A kind of Hericium erinaceus culture material compatibility, has following component and quality proportioning: vinegar grain 40%-50%, broad bean skin 25%-35%, wheat bran 10%-20%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2%, be all the quality of dry-matter, proportioning sum is 100%.

Vinegar grain: fresh vinegar grain, acid higher, be spread out on concrete floor or on plastics film and ted, tart flavour is vapored away as far as possible, make moisture content drop to below 12%-15% simultaneously, dry for subsequent use.

Broad bean skin, through drying, is ground into below 5mm (particle diameter), for subsequent use.

Wheat bran: be wheat skin remaining during wheat flour milling.

Semen Maydis powder, calcium superphosphate, gypsum are common commercially available product.

The making method of planting material, comprises the steps:

1. prewet: vinegar grain is mixed with broad bean skin siccative, prewets 2 hours, make the water content of mixture remain on 50%-55%;

2. spice: by wheat bran, Semen Maydis powder, calcium superphosphate, gypsum, joins in the mixture of having prewetted, stirs, then slowly add water, and limit edged is mixed, and fully stirs evenly, and makes the water content of planting material remain on 63%-65%;

3. bottle, beat and plant bacterium hole: planting material is loaded 1100ml × 78mmPP Plastic Bottle, stamp and plant bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;

4. sterilizing, cooling: planting material is carried out autoclaving, first sterilizing 1h at 100 DEG C, then rises to 121 DEG C of sterilizing 1.5h, naturally cools to less than 25 DEG C after sterilizing;

Planting material is after access bacterial classification, and culture temperature controls at 20 DEG C ~ 22 DEG C, and humid control is 60% ~ 75%, and through the cultivation of 20d ~ 23d, mycelia covers with bottle, can enter management of producing mushroom.

The present invention for main raw material, makes Hericium erinaceus culture material with Brewing industry tankage vinegar grain, broad bean skin.Its advantage is: one is gather materials on the spot, and reduces production cost; Two is that vinegar grain feedstock acidity is suitable for, comprehensive nutrition, and after arranging in pairs or groups with broad bean skin, planting material ventilation property, retentiveness are good, and mycelium growth vigor is vigorous, sporophore growth is neat, and output is high, quality better; Three is utilize poor slag for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers., has widened culturing raw material channel, has improve resource utilization, added the income of brewing enterprise simultaneously.

Embodiment

Mode 1: in the present embodiment, Hericium erinaceus culture material comprises following component and quality proportioning: vinegar grain 40%, broad bean skin 30%, wheat bran 20%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2%, be all the quality of dry-matter, proportioning sum is 100%.

Making method:

1. prewet: vinegar grain is mixed with broad bean skin siccative, prewets 2 hours, make the water content of mixture remain on 50%-55%;

2. spice: by wheat bran, Semen Maydis powder, calcium superphosphate, gypsum, joins in the mixture of having prewetted, stirs, then slowly add water, and limit edged is mixed, and fully stirs evenly, and makes the water content of planting material remain on 63%-65%;

3. bottle, beat and plant bacterium hole: planting material is loaded 1100ml × 78mmPP Plastic Bottle, stamp and plant bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;

4. sterilizing, cooling: planting material is carried out autoclaving, first sterilizing 1h at 100 DEG C, then rises to 121 DEG C of sterilizing 1.5h, naturally cools to less than 25 DEG C after sterilizing;

Planting material is after access bacterial classification, and culture temperature controls at 20 DEG C ~ 22 DEG C, and humid control is 60% ~ 75%, and through the cultivation of 20d ~ 23d, mycelia covers with bottle, can enter management of producing mushroom.

Mode 2: in the present embodiment, Hericium erinaceus culture material comprises following component and quality proportioning: vinegar grain 46%, broad bean skin 28%, wheat bran 16%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2%, be all the quality of dry-matter, proportioning sum is 100%.Making method reference example 1.

Mode 3: in the present embodiment, Hericium erinaceus culture material comprises following component and quality proportioning: vinegar grain 48%, broad bean skin 32%, wheat bran 10%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2%, be all the quality of dry-matter, proportioning sum is 100%.Making method reference example 1.

Mode 4 (control group 1): Hericium erinaceus culture material comprises following component and quality proportioning: cotton seed hulls 78%, wheat bran 20%, calcium superphosphate 1%, terra alba 1%, be all the quality of dry-matter, proportioning sum is 100%.

Mode 5 (control group 2): Hericium erinaceus culture material comprises following component and quality proportioning: wood chip 78%, wheat bran 20%, calcium superphosphate 1%, terra alba 1%, be all the quality of dry-matter, proportioning sum is 100%.

As seen from the table: the formula group being major ingredient with vinegar grain of all modes, mycelial growth rate is obviously better than control group, full bottle number of days generally shortens, and this nutrition that may be vinegar grain contains is more conducive to the utilization of Hericium erinaceus (Bull. Ex Fr.) Pers., also may there is the chemical composition stimulating hericium mycelium growth in vinegar grain; All modes with vinegar grain be the formula group biological efficiency of major ingredient be significantly higher than with wood chip be major ingredient formula group, be close with the formula group taking cotton seed hulls as major ingredient.

The planting material of table different ingredients is on the impact of Hericium erinaceus (Bull. Ex Fr.) Pers. output

Claims (1)

1. a making method for Hericium erinaceus culture material, is characterized in that: described planting material is containing, for example lower component and quality proportioning: vinegar grain 46%, broad bean skin 28%, wheat bran 16%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2%, be all the quality of dry-matter, proportioning sum is 100%; Described vinegar grain is the waste after brewery's wine vinegar; Described broad bean skin is through drying, being ground into the particle of below particle diameter 5mm; The making method of described planting material is:

1. prewet: vinegar grain is mixed with broad bean skin siccative, prewets 2 hours, make the water content of mixture remain on 50%-55%;

2. spice: by wheat bran, Semen Maydis powder, calcium superphosphate, gypsum, joins in the mixture of having prewetted, stirs, then slowly add water, and limit edged is mixed, and fully stirs evenly, and makes the water content of planting material remain on 63%-65%;

3. bottle, beat and plant bacterium hole: planting material is loaded 1100ml × 78mmPP Plastic Bottle, stamp and plant bacterium hole, planting aperture, bacterium hole is 16mm, then covers the bottle cap of the ventilative sponge of band;

4. sterilizing, cooling: planting material is carried out autoclaving, first sterilizing 1h at 100 DEG C, then rises to 121 DEG C of sterilizing 1.5h, naturally cools to less than 25 DEG C after sterilizing;

After made planting material access cultivar, culture temperature controls at 20 DEG C-22 DEG C, and humid control is at 60%-75%, and through the cultivation of 20d-23d, mycelia covers with bottle, can enter management of producing mushroom.