CN103877606A - Absorbable bleeding-stopping compound sponge and preparation method thereof - Google Patents
Absorbable bleeding-stopping compound sponge and preparation method thereof Download PDFInfo
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- CN103877606A CN103877606A CN201410032250.3A CN201410032250A CN103877606A CN 103877606 A CN103877606 A CN 103877606A CN 201410032250 A CN201410032250 A CN 201410032250A CN 103877606 A CN103877606 A CN 103877606A
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Abstract
The invention discloses an absorbable bleeding-stopping compound sponge and a preparation method thereof. The absorbable bleeding-stopping compound sponge comprises sodium alginate, carboxymethyl cellulose sodium and a crosslinking agent serving as raw materials. In the preparation method, a freeze drying method is adopted. Carboxymethyl cellulose sodium and sodium alginate in the compound sponge have strong interaction and high compatibility, so that the compound sponge has the advantages of good absorptive bleeding-stopping effect, in-vivo degradability, no cytotoxicity, no immunogenicity, no increase in the infection probability and no influence on tissue healing, and is not completely dependent on the blood coagulation process of an organism. In the method, a compound surface is prepared by adopting the freeze drying method, so that the preparation method has the advantages of simple and mature technological process, low cost, short preparation period, no organic residue, environment-friendly preparation process, high practicability and wide application prospect.
Description
Technical field
The invention belongs to field of medical products, be specifically related to composite sponge of a kind of absorbable hemostatic and preparation method thereof.
Background technology
In the processes such as surgery and trauma operation or war wound, hemorrhage and oozing of blood is the difficult problem often running in operation, thus absorbable hemostatic material have can body in absorption need not take out focus little, easy and simple to handle to tissue injury, that the feature such as rapid of stopping blooding has become current surgical field.But absorbable hemostatic material of the prior art still has certain cytotoxic immune originality, affects organization healing, can not meet the demand of clinical use completely.
Summary of the invention
The object of the invention is to overcome prior art defect, a kind of composite sponge of absorbable hemostatic is provided.
Another object of the present invention is to provide the preparation method of above-mentioned composite sponge.
Concrete technical scheme of the present invention is as follows:
A composite sponge for absorbable hemostatic, its raw material comprises sodium alginate, Carboxymethyl cellulose sodium and cross-linking agent.
In a preferred embodiment of the invention, described cross-linking agent is genipin or glutaraldehyde.
Another technical scheme of the present invention is as follows:
A preparation method for the composite sponge of above-mentioned absorbable hemostatic, adopts freeze-drying.
In a preferred embodiment of the invention, comprise the steps:
(1) after the aqueous solution of Carboxymethyl cellulose sodium and sodium alginate is evenly mixed, add cross-linking agent solution to stir and standing and reacting froth breaking, obtain compound;
(2) compound step (1) being made carries out lyophilization after putting into the freezing icing molding of mould, obtains described composite sponge.
In a preferred embodiment of the invention, the concentration of the aqueous solution of described Carboxymethyl cellulose sodium and sodium alginate is 1～4%, and both volume ratios are 1～9:1, the concentration thing 0.5～0.7% of described cross-linking agent solution.
In a preferred embodiment of the invention, described freezing icing being shaped to first placed 3～6 hours at-20～-18 ℃, then places 3～6 hours at-8～-4 ℃.
In a preferred embodiment of the invention, described cryodesiccated temperature is-55 ℃, and the time is 36～48 hours.
The invention has the beneficial effects as follows:
1, the composite sponge of absorbable hemostatic of the present invention, its raw material comprises sodium alginate, Carboxymethyl cellulose sodium and cross-linking agent, sodium alginate (Alginate, ALG) be the sodium salt of a kind of polyanionic polysaccharide (alginic acid) of extracting from the Thallus Laminariae (Thallus Eckloniae) of Brown algae or Alga Sgrgassi Enerves, himself negative charge density is very high, soluble in water, have nontoxic, good biodegradation and biocompatibility, by 1, a kind of linear polymer of gather-beta-D-mannuronic acid (M) of 4-and a-L-guluronic acid (G) composition, it has unique mass transfer performances, hydrophilic, stability, gelling, oil resistivity, the characteristics such as film property, sodium carboxymethyl cellulose (Carboxymethyl cellulose, CMC), is first made in 1918 by Germany, and gets permission patent and be seen in generation in nineteen twenty-one.Sodium carboxymethyl cellulose is white or micro-yellow powder, granular or fibrous solid, odorless, tasteless, nontoxic.CMC is a kind of macromolecular chemistry material, can imbibition, when swelling in water, can form transparent sticky glue, aspect acid-base value, show as neutrality, solid CMC is all more stable to light and temperature, can be kept at for a long time in dry environment, and CMC has the performances such as the good bonding of moisture-absorption characteristics, thickening, film forming, maintenance moisture;
2, the sodium carboxymethyl cellulose in composite sponge of the present invention and sodium alginate have stronger interaction and the good compatibility, absorption haemostatic effect is good, can vivo degradation, no cytotoxicity, non-immunogenicity, do not increase Infection probability, do not affect organization healing, not exclusively rely on body coagulation process simultaneously.
3, method of the present invention adopts freeze-drying to prepare composite surface, and technical process is simple ripe, with low cost, and manufacturing cycle is short, without organic residue, and preparation process environmental protection, thereby there is practical and wide application prospect.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of the prepared composite sponge of the embodiment of the present invention 1.
The specific embodiment
By reference to the accompanying drawings technical scheme of the present invention is further detailed and is described by the specific embodiment below.
Embodiment 1:
Configure sodium alginate and sodium carboxymethyl cellulose solution that a concentration is 1%-4%, 2.5% genipin or glutaraldehyde solution; By above-mentioned sodium alginate and sodium carboxymethyl cellulose solution according to the volume ratio stirring and evenly mixing of 1:1, add 0.05% 2.5% genipin or glutaraldehyde solution 1000r/min to stir after 30min, room temperature leaves standstill 2 hours above (bubble of not cooling down adopts nitrogen piping and druming must disappear to the greatest extent), obtains compound; Pack above-mentioned compound into special Teflon mould, put into first and within 3 hours, put into again-8 ℃ of above molding of freezing in 3 hours at-20 ℃; Finally, this sample is put into-55 ℃ of freezer dryer lyophilizations and within 48 hours, obtained described composite sponge (as shown in Figure 1);
Adult kunming mice (20-30g), is provided by Foochow Wu Shi animal, before experiment, raises and conforms for one week.In experimentation, every group of 10 of material experiment mice (random packet), the pentobarbital sodium solution of employing 2% carries out intraperitoneal injection of anesthesia (40-50mg/kg), 8%Na to mice
2s sloughs huckle hair; Cut off the about 1cm of skin along femoral artery trend, operating scissors is cut off femoral artery, starts with manual time-keeping Cotton Gossypii (quality m after about 10s
3) wipe out the m that weighs after the blood of wound
4, after by diameter 1 × 1cm quality m
1material cover on wound, push down wound 10s completely with forefinger and be convenient to more effective hemostasis, when blood infiltrate no longer be outward considered as hemostasis stop, recording bleeding stopping period t; After half an hour, opening material on wound does not have that secondary is hemorrhage to be considered as successfully, weighs the quality m that takes dressing off
2; Whole hemostasis, adopts gauze and the Avitene of The Budd Co. of commercially available U.S. sponge (to the results are shown in Table one) in contrast;
T (bleeding stopping period)=t-10s; M (blood loss)=(m
4-m
3)+(m
2-m
1);
Embodiment 2:
The present embodiment is substantially the same manner as Example 1, and difference is that the volume ratio of sodium alginate and carboxymethyl cellulose is 9:1, makes composite sponge, and carries out hemostasis experiment (the results are shown in Table) in body;
Table one: material strand artery trauma hemostasis result table
Embodiment 3:
The present embodiment is substantially the same manner as Example 2, and difference is that prepared sponge adopts liver trauma evaluation.
Adult kunming mice (20-30g), is provided by Foochow Wu Shi animal, before experiment, raises and conforms for one week.In experimentation, every group of 10 of material experiment mice (random packet), the pentobarbital sodium solution of employing 2% carries out intraperitoneal injection of anesthesia (40-50mg/kg), 8%Na to mice
2s sloughs huckle hair; Cut off the about 1cm of skin along femoral artery trend, operating scissors is cut off femoral artery, starts, with manual time-keeping, to wait 10s Cotton Gossypii (quality m
3) wipe out the m that weighs after the blood of wound
4, after by diameter 1cm × 1 quality m
1material cover on wound, push down wound 10s completely with forefinger and be convenient to more effective hemostasis, when blood infiltrate no longer be outward considered as hemostasis stop, recording bleeding stopping period t; After half an hour, opening material on wound does not have that secondary is hemorrhage to be considered as successfully, weighs the quality m that takes dressing off
2; Whole hemostasis, adopts gauze and the Avitene of The Budd Co. of commercially available U.S. sponge in contrast, and result is as shown in table 2 below;
T (bleeding stopping period)=t-10s; M (blood loss)=(m
4-m
3)+(m
2-m
1);
Table 2: material liver trauma hemostasis result table
Embodiment 4:
The present embodiment is substantially the same manner as Example 1, and difference is after preparing composite sponge, and sponge is immersed in to 4%CaCl
2half an hour in solution, after again-20 ℃ and-80 ℃ of pre-coolings operation, again carry out lyophilizing operation, finally make CMC-ALG (Ca
2+) composite sponge; And carry out femoral hemostasis test data sheet bleeding stopping period, result is as shown in table 3 below.
T (bleeding stopping period)=t-10s;
Table 3: material stops femoral artery wound hemostasis timetable
The above, be only preferred embodiment of the present invention, therefore can not limit according to this scope of the invention process, the equivalence done according to the scope of the claims of the present invention and description changes and modifies, and all should still belong in the scope that the present invention contains.
Claims (7)
1. a composite sponge for absorbable hemostatic, is characterized in that: its raw material comprises sodium alginate, Carboxymethyl cellulose sodium and cross-linking agent.
2. the composite sponge of a kind of absorbable hemostatic as claimed in claim 1, is characterized in that: described cross-linking agent is genipin or glutaraldehyde.
3. a preparation method for the composite sponge of the absorbable hemostatic described in claim 1 or 2, is characterized in that: adopt freeze-drying.
4. the preparation method of the composite sponge of absorbable hemostatic as claimed in claim 3, is characterized in that: comprise the steps:
(1) after the aqueous solution of Carboxymethyl cellulose sodium and sodium alginate is evenly mixed, add cross-linking agent solution to stir and standing and reacting froth breaking, obtain compound;
(2) compound step (1) being made carries out lyophilization after putting into the freezing icing molding of mould, obtains described composite sponge.
5. the preparation method of the composite sponge of absorbable hemostatic as claimed in claim 4, it is characterized in that: the concentration of the aqueous solution of described Carboxymethyl cellulose sodium and sodium alginate is 1～4%, both volume ratios are 1～9:1, the concentration thing 0.5～0.7% of described cross-linking agent solution.
6. the preparation method of the composite sponge of absorbable hemostatic as claimed in claim 5, is characterized in that: described freezing icing being shaped to first placed 3～6 hours at-20～-18 ℃, then places 3～6 hours at-8～-4 ℃.
7. the preparation method of the composite sponge of absorbable hemostatic as claimed in claim 6, is characterized in that: described cryodesiccated temperature is-55 ℃, and the time is 36～48 hours.
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| CN201410032250.3A CN103877606A (en) | 2014-01-23 | 2014-01-23 | Absorbable bleeding-stopping compound sponge and preparation method thereof |
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| CN201410032250.3A CN103877606A (en) | 2014-01-23 | 2014-01-23 | Absorbable bleeding-stopping compound sponge and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105107010A (en) * | 2015-09-23 | 2015-12-02 | 江苏蓝湾生物科技有限公司 | Preparation method of oxidized cellulose external applying material with anti-microbial and detumescence effect |
| CN105343924A (en) * | 2015-11-30 | 2016-02-24 | 北京化工大学 | Method of using dopamine for rapidly crosslinking chitosan to prepare hemostatic sponge |
| CN105400214A (en) * | 2015-11-30 | 2016-03-16 | 北京化工大学 | Method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin |
| CN109276745A (en) * | 2018-12-07 | 2019-01-29 | 上海新华瑞思医疗科技有限公司 | A kind of cellulose combine dressing of high wet strength and preparation method thereof |
| CN112076343A (en) * | 2020-08-14 | 2020-12-15 | 中国人民解放军南部战区总医院 | Alginate-carboxymethyl cellulose gel sponge and preparation method and application thereof |
| CN115645601A (en) * | 2022-10-28 | 2023-01-31 | 浙江医鼎医用敷料有限公司 | Gradient-structure antibacterial hydrogel dressing and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580134A (en) * | 2011-12-20 | 2012-07-18 | 山东省医疗器械研究所 | Biological and haemostatic wound dressing and preparation method thereof |
-
2014
- 2014-01-23 CN CN201410032250.3A patent/CN103877606A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580134A (en) * | 2011-12-20 | 2012-07-18 | 山东省医疗器械研究所 | Biological and haemostatic wound dressing and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105107010A (en) * | 2015-09-23 | 2015-12-02 | 江苏蓝湾生物科技有限公司 | Preparation method of oxidized cellulose external applying material with anti-microbial and detumescence effect |
| CN105343924A (en) * | 2015-11-30 | 2016-02-24 | 北京化工大学 | Method of using dopamine for rapidly crosslinking chitosan to prepare hemostatic sponge |
| CN105400214A (en) * | 2015-11-30 | 2016-03-16 | 北京化工大学 | Method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin |
| CN109276745A (en) * | 2018-12-07 | 2019-01-29 | 上海新华瑞思医疗科技有限公司 | A kind of cellulose combine dressing of high wet strength and preparation method thereof |
| CN112076343A (en) * | 2020-08-14 | 2020-12-15 | 中国人民解放军南部战区总医院 | Alginate-carboxymethyl cellulose gel sponge and preparation method and application thereof |
| CN115645601A (en) * | 2022-10-28 | 2023-01-31 | 浙江医鼎医用敷料有限公司 | Gradient-structure antibacterial hydrogel dressing and preparation method and application thereof |
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Application publication date: 20140625 |