CN103876145B - A kind of probiotics micro-ecological tablet and its preparation method - Google Patents
A kind of probiotics micro-ecological tablet and its preparation method Download PDFInfo
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- CN103876145B CN103876145B CN201410129017.7A CN201410129017A CN103876145B CN 103876145 B CN103876145 B CN 103876145B CN 201410129017 A CN201410129017 A CN 201410129017A CN 103876145 B CN103876145 B CN 103876145B
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- 239000006041 probiotic Substances 0.000 title claims abstract description 68
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 68
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- 239000000843 powder Substances 0.000 claims abstract description 69
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 43
- 235000015141 kefir Nutrition 0.000 claims abstract description 38
- 241000894006 Bacteria Species 0.000 claims abstract description 33
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 30
- 238000000855 fermentation Methods 0.000 claims abstract description 29
- 230000004151 fermentation Effects 0.000 claims abstract description 29
- 230000001580 bacterial effect Effects 0.000 claims abstract description 19
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims abstract description 18
- 230000000529 probiotic effect Effects 0.000 claims abstract description 17
- 235000019359 magnesium stearate Nutrition 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 8
- 230000001681 protective effect Effects 0.000 claims abstract description 6
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 14
- 239000008101 lactose Substances 0.000 claims description 14
- 235000013336 milk Nutrition 0.000 claims description 14
- 239000008267 milk Substances 0.000 claims description 14
- 210000004080 milk Anatomy 0.000 claims description 14
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 12
- 241000186000 Bifidobacterium Species 0.000 claims description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 108010046377 Whey Proteins Proteins 0.000 claims description 12
- 102000007544 Whey Proteins Human genes 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 235000020183 skimmed milk Nutrition 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000008187 granular material Substances 0.000 claims description 7
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 229920001202 Inulin Polymers 0.000 claims description 6
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 6
- 239000005862 Whey Substances 0.000 claims description 6
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 6
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 238000007689 inspection Methods 0.000 claims description 6
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 6
- 229940029339 inulin Drugs 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 6
- 229920001542 oligosaccharide Polymers 0.000 claims description 6
- 150000002482 oligosaccharides Chemical class 0.000 claims description 6
- 229940073490 sodium glutamate Drugs 0.000 claims description 6
- 235000021119 whey protein Nutrition 0.000 claims description 6
- 238000001694 spray drying Methods 0.000 claims description 5
- 241000186012 Bifidobacterium breve Species 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 208000010040 Sprains and Strains Diseases 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 230000029087 digestion Effects 0.000 abstract description 3
- 239000000796 flavoring agent Substances 0.000 abstract description 2
- 235000019634 flavors Nutrition 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 230000000050 nutritive effect Effects 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 15
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 239000002131 composite material Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 5
- 238000001816 cooling Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 238000009472 formulation Methods 0.000 description 3
- 244000005709 gut microbiome Species 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 1
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
A kind of probiotics micro-ecological tablet of the present invention and its preparation method, belong to the technical field of protective foods and processing. Probiotics micro-ecological tablet is probiotics bacterial powder 90%��98% by mass, oligofructose 1%��9%, Magnesium Stearate 0.1%��1%. Preparation is activated by bifidus bacillus, and prepares Kefir fermenting agent; Again the two is obtained through mixed fermentation, cryoconcentration drying, batch mixing compressing tablet. Mixed fermentation is inoculated by probiotic bacterium mixed strains, ferments 16��24 hours under 28��36 DEG C of conditions, the ratio 1��3:1 of Kefir fermenting agent and bifidus bacillus in mixed strains. Kefir fermenting agent and bifidus bacillus are carried out high-density mixed fermentation by the present invention, and bacterial classification is various, stable storing, and the viable count in tablet reaches 2,000,000,000 more than cfu/g respectively; It is possible not only to improve digestion ability, also enhances the colonization ability of probiotic bacterium in enteron aisle simultaneously; There is nutritive value height, the features such as unique flavor.
Description
Technical field
The present invention relates to a kind of probiotics micro-ecological tablet and its preparation method, belong to the technical field of protective foods and processing.
Background technology
The effects such as probiotics micro-ecological tablet, as the one of probiotics micro-ecological formulation, has adjustment intestinal microflora, improves human body microecological balance, the growth and breeding of the pathogenic micro-organism that suppression is harmful in human body. Effective constituent in probiotics micro-ecological tablet is probiotics viable bacteria, and probiotic bacterium common at present has milk-acid bacteria, bifidus bacillus, lactobacillus bulgaricus, thermophilus streptococcus etc. Probiotics micro-ecological formulation commercially available at present is mainly solid granules or capsule, the probiotics bacterial powder wherein contained is become through composite post-treatment with auxiliary material by the bacterium powder of single culture mostly, bacterium powder is after repeatedly processing, and spawn activity declines, and is not enough to reach the health-care effect of expection.
The present invention is by Kefir fermenting agent and bifidus bacillus through high-density culture, after mixed fermentation, after cryoconcentration drying, probiotics micro-ecological tablet made by compressing tablet, avoids the repeatedly processing of bacterium powder, it is to increase the viable bacteria content of probiotic bacterium. Secondly, probiotics micro-ecological formulation commercially available at present mostly is that single culture is composite and become, and this Tiny ecosystem tablet contains the multiple probiotic bacterium in Kefir fermenting agent, and with bifidus bacillus mixed fermentation after make, enrich the kind of probiotic bacterium, it is to increase the health-care effect of product.
Summary of the invention
It is an object of the invention to provide a kind of probiotics micro-ecological tablet and its preparation method, product contains various active probiotic bacterium, has adjustment intestinal microflora balance, and promoting digestion absorbs, enhancing body immunizing power, the effects such as treatment diarrhoea and constipation.
The present invention is realized by following technique means:
A kind of probiotics micro-ecological tablet, component is by mass percentage, probiotics bacterial powder 90%��98%, oligofructose 1%��9%, Magnesium Stearate 0.1%��1%.
In above-mentioned tablet, probiotic bacterium is made up of the composite bacteria in Kefir fermenting agent and bifidus bacillus. By mass in Kefir fermenting agent composite bacteria bifidus bacillus=1��3 1.
Probiotics bacterial powder is that mixed fermentation, obtains after cryoconcentration drying by the composite bacteria (milk-acid bacteria, yeast etc.) in Kefir fermenting agent, bifidus bacillus through high-density culture.
In above-mentioned tablet, bifidus bacillus is made up of 1��3 kind in bifidobacteria infantis, bifidus longum bb, bifidobacterium breve, bifidobacterium adolescentis. During containing 2��3 kinds of bifidus bacilluss, the mass ratio of various bifidus bacillus can be random, and concrete ratio depends on the ratio of various activation bifidus bacillus mixing used in preparation.
In above-mentioned tablet, viable bacteria concentration is greater than 6,000,000,000 cfu/g.
A preparation method for probiotics micro-ecological tablet, first uses MRS lactose medium to be activated by bifidus bacillus, it may also be useful to skimming milk inoculation Kefir granule prepares Kefir fermenting agent; It is characterized in that, then obtain through mixed fermentation, cryoconcentration drying, batch mixing compressing tablet;
Described mixed fermentation prepares substratum by following process, and substratum component by weight percentage has, condensed whey protein 2%��10%, glucose 1%��10%, yeast powder 0.5%��1%, calcium carbonate 0.4%��1%, magnesium sulfate 0.01%��0.06%, manganous sulfate 0.001%��0.01%, whey powder 1%��10%, inulin 0.5%��3%, panaxan 0.04%��1%, soybean oligosaccharide 0.02%��1%, and surplus is distilled water; By each components dissolved in distilled water, regulate pH to be 6.5��7.5, under 90��115 DEG C of conditions, it is incubated 10��30min sterilization; It is cooled to 20��30 DEG C of mixed strainss by Kefir fermenting agent and bifidus bacillus to inoculate by the 3%��10% of culture volume, under 28��36 DEG C of conditions, ferment 16��24 hours, obtaining probiotic mixed fermentation liquid, wherein the inoculative proportion of Kefir fermenting agent and bifidus bacillus is Kefir fermenting agent: bifidus bacillus=1��3:1;
Described cryoconcentration is dry, be by probiotic mixed fermentation liquid when 3000��6000r/min, stir centrifugal 15��30min, collect concentrated thalline; In concentrated thalline, add protective material again, carry out vacuum lyophilization by existing method after pre-freeze or be 80��120 DEG C in inlet temperature, when air outlet temperature 50��70 DEG C, carry out low temperature spray drying, obtain probiotics bacterial powder;
Described batch mixing compressing tablet 90%��98% probiotics bacterial powder, 1%��9% oligofructose, 0.1%��1% Magnesium Stearate is mixed according to mass percent, and probiotics micro-ecological tablet made by compressing tablet.
The finished product finally made is the composite probiotics micro-ecological tablet that unit viable count is greater than 6,000,000,000 cfu/g.
Described bifidus bacillus activates, can be undertaken by prior art, can also be undertaken by following process, frozen Bifidobacteria powder is inoculated in MRS lactose medium, after 37 DEG C of cultivation 24h, meeting the standard form of bifidus bacillus through mirror inspection, growth conditions is good, it is inoculated in MRS lactose medium by inoculum size 3%��6% again to activate, cultivates 18��24h for 37 DEG C.
Above-mentioned Bifidobacteria powder, it is possible to be that bifidobacteria infantis powder, bifidus longum bb powder, bifidobacterium breve powder are or/and bifidobacterium adolescentis powder; Use 2��3 kinds of bacterium powder time, activate respectively be mixed in proportion again obtain activation bifidus bacillus, for Kefir fermenting agent mixed fermentation.
Described prepares Kefir fermenting agent, can be undertaken by prior art, can also be undertaken by following process, skimmed milk powder is used to be made into the skimming milk solution of 10%, through 90��115 DEG C, after 10��30min sterilization, it is cooled to 20��30 DEG C, according to 0.1%��0.5% inoculation Kefir granule of skimming milk volume, ferment 14��18h when 23��28 DEG C.
In cryoconcentration drying process, described protective material, is be radix by probiotic mixed fermentation liquid weight, is made up of skimmed milk powder 10%��20%, trehalose 1%��10%, Sodium Glutamate 1%��10%, glycerine 1%��5%.
In batch mixing tableting processes, described compressing tablet, it is possible to use tabletting machine, carries out when pressure is 4000��10000N. Can be pressed into diameter 6��12mm, sheet weighs 0.3��0.5g, hardness 8��10kg/cm2Tablet.
The useful effect of the present invention is:
1) Kefir fermenting agent and bifidus bacillus are carried out high-density mixed fermentation by the present invention, and bacterial classification is various, stable storing, and the viable count in tablet can reach 2,000,000,000 more than cfu/g respectively;
2) after Kefir fermenting agent and bifidus bacillus mixed fermentation, it is possible not only to improve digestion ability, also enhances the colonization ability of probiotic bacterium in enteron aisle simultaneously, enriched intestinal microflora, improve the microecological balance of enteron aisle, maintenance intestinal health is had good effect;
3) the present invention contains abundant probiotic bacterium and active metabolite thereof, and with the addition of oligofructose, has nutritive value height, the features such as unique flavor.
Embodiment
Following examples are the further explanations to the present invention, instead of limitation of the present invention.
Embodiment 1:
1) preparation of probiotics leaven
1. the preparation of bifidus bacillus
Frozen bifidobacterium adolescentis bacterium powder is inoculated in MRS lactose medium, 37 DEG C, after cultivating 24h, meet the standard form of bifidus bacillus through mirror inspection, growth conditions is good, is inoculated in MRS lactose medium by inoculum size 3% and activates, 37 DEG C, cultivate 24h and save backup.
2. the preparation of Kefir fermenting agent
Use skimmed milk powder to be made into the skimming milk solution of 10%, through 115 DEG C, after 30min sterilization, it is cooled to 25 DEG C, according to 0.1% inoculation Kefir granule of skimming milk volume, save backup after the 18h that ferments when 23 DEG C.
2) mixed fermentation
Getting condensed whey protein 10g, glucose 6.7g, yeast powder 3.8g, calcium carbonate 3.4g, magnesium sulfate 0.2g, manganous sulfate 0.015g, whey powder 14g, inulin 2.5g, panaxan 0.5g, soybean oligosaccharide 1g, adding distilled water to total mass is 500g. Regulating medium pH to be 7.0 after fully dissolving, sterilization conditions is be incubated 30min under 115 DEG C of conditions, is cooled to 30 DEG C for subsequent use.
To the high-density culture medium inoculation after cooling, by 3% inoculum size, Kefir fermenting agent: bifidobacterium adolescentis=2:1,28 DEG C, fermentation 24h.
3) preparation of bacterium powder
Being stirred evenly by obtained fermented liquid, under 20 DEG C of conditions, rotating speed is 6000 revs/min, and the time is 15min centrifugal treating, collects concentrated thalline; The thalline of centrifugal collection, add skimmed milk powder 10%, trehalose 5%, Sodium Glutamate 1%, glycerine 3%, mix, sterilized water adjustment dry substance concentration is 30��40%, it it is 80��120 DEG C in inlet temperature, when air outlet temperature 50��70 DEG C, carry out low temperature spray drying, obtain probiotics bacterial powder.
4) compressing tablet
According to mass percent by 98% probiotics bacterial powder, 1.5% oligofructose, 0.5% Magnesium Stearate mixes, and then utilizes tabletting machine, is 4000N condition lower sheeting at pressure, makes probiotics micro-ecological tablet. Wherein, the diameter of tablet is 8mm, sheet weight: 0.3g, hardness: 8kg/cm2. The technical indicator of Tiny ecosystem tablet is: lactic acid bacteria number >=3,000,000,000 cfu/g, yeast >=2,000,000,000 cfu/g, bifidus bacillus >=2,000,000,000 cfu/g.
Embodiment 2
1) preparation of probiotics leaven
1. the preparation of bifidus bacillus
Frozen bifidobacterium adolescentis bacterium powder and bifidobacteria infantis bacterium powder are inoculated in MRS lactose medium respectively, 37 DEG C, after cultivating 24h, the standard form of bifidus bacillus is met through mirror inspection, growth conditions is good, by inoculum size 5%, is inoculated in MRS lactose medium respectively and activates, 37 DEG C, cultivate 20h. Bifidobacterium adolescentis and bifidobacteria infantis are mixed according to volume ratio 1:1, saves backup.
2. the preparation of Kefir fermenting agent
Use skimmed milk powder to be made into the skimming milk solution of 10%, through 115 DEG C, after 30min sterilization, it is cooled to 23 DEG C, according to 0.3% inoculation Kefir grains throw type leaven of skimming milk volume, save backup after the 16h that ferments when 26 DEG C.
2) mixed fermentation
Getting condensed whey protein 18g, glucose 5g, yeast powder 4.29g, calcium carbonate 4.0g, magnesium sulfate 0.3g, manganous sulfate 0.027g, whey powder 10g, inulin 5g, panaxan 1g, soybean oligosaccharide 0.5g, adding distilled water to total mass is 500g. Regulating medium pH to be 7.0 after fully dissolving, sterilization conditions is be incubated 20min under 115 DEG C of conditions, is cooled to 25 DEG C for subsequent use.
To the high-density culture medium inoculation after cooling, by 5% inoculum size, Kefir fermenting agent: bifidus bacillus=1:1,30 DEG C, fermentation 20h.
3) preparation of bacterium powder
Being stirred evenly by obtained fermented liquid, under 15 DEG C of conditions, rotating speed is 4000 revs/min, and the time is 20min centrifugal treating, collects concentrated thalline; The thalline of centrifugal collection, adds skimmed milk powder 15%, trehalose 1%, Sodium Glutamate 7%, glycerine 5%, mixes, and after pre-freeze 8h, after putting into the dry 24h of low-temperature freeze-drying machine, obtains probiotics bacterial powder.
4) compressing tablet
According to mass percent by 96% probiotics bacterial powder, 3% oligofructose, 1% Magnesium Stearate mixes, and then utilizes tabletting machine, is 6000N condition lower sheeting at pressure, makes probiotics micro-ecological tablet. Wherein, the diameter of tablet is 10mm, sheet weight: 0.4g, hardness: 9kg/cm2. The technical indicator of Tiny ecosystem tablet is: lactic acid bacteria number >=3,000,000,000 cfu/g, yeast >=2,000,000,000 cfu/g, bifidus bacillus >=2,000,000,000 cfu/g.
Embodiment 3
1) preparation of probiotics leaven
1. the preparation of bifidus bacillus
By frozen bifidobacterium adolescentis bacterium powder, bifidus longum bb bacterium powder and bifidobacteria infantis bacterium powder are inoculated in MRS lactose medium respectively, 37 DEG C, after cultivating 24h, meeting the standard form of bifidus bacillus through mirror inspection, growth conditions is good, by inoculum size 6%, it is inoculated in MRS lactose medium respectively to activate, 37 DEG C, cultivates 20h. By bifidobacterium adolescentis, bifidus longum bb and bifidobacteria infantis, according to volume ratio 2:1:1 mixing, save backup.
2. the preparation of Kefir fermenting agent
Use skimmed milk powder to be made into the skimming milk solution of 10%, through 115 DEG C, after 20min sterilization, it is cooled to 20 DEG C, according to 0.5% inoculation Kefir grains throw type leaven of skimming milk volume, save backup after the 14h that ferments when 28 DEG C.
2) mixed fermentation
Getting condensed whey protein 20g, glucose 8.0g, yeast powder 5g, calcium carbonate 4g, magnesium sulfate 0.1g, manganous sulfate 0.020g, whey powder 10g, inulin 7g, panaxan 0.2g, soybean oligosaccharide 0.1g, adding distilled water to total mass is 500g. Regulating medium pH to be 7.0 after fully dissolving, sterilization conditions is be incubated 30min under 95 DEG C of conditions, is cooled to 25 DEG C for subsequent use.
To the high-density culture medium inoculation after cooling, by 6% inoculum size, Kefir fermenting agent: bifidus bacillus=3:1,32 DEG C, fermentation 18h.
3) preparation of bacterium powder
Being stirred evenly by obtained fermented liquid, under 10 DEG C of conditions, rotating speed is 3000 revs/min, and the time is 30min centrifugal treating, collects concentrated thalline; The thalline of centrifugal collection, add skimmed milk powder 20%, trehalose 6%, Sodium Glutamate 5%, glycerine 1%, mix, sterilized water adjustment dry substance concentration is 30��40%, it it is 80��120 DEG C in inlet temperature, when air outlet temperature 50��70 DEG C, carry out low temperature spray drying, obtain probiotics bacterial powder.
4) compressing tablet
According to mass percent by 95% probiotics bacterial powder, 4.7% oligofructose, 0.3% Magnesium Stearate mixes, and then utilizes tabletting machine, is 8000N condition lower sheeting at pressure, makes probiotics micro-ecological tablet. Wherein, the diameter of tablet is 12mm, sheet weight: 0.5g, hardness: 10kg/cm2. The technical indicator of Tiny ecosystem tablet is: lactic acid bacteria number >=3,000,000,000 cfu/g, yeast >=2,000,000,000 cfu/g, bifidus bacillus >=2,000,000,000 cfu/g.
Embodiment 4
The probiotics micro-ecological tablet of the present invention all can be obtained by following materials and processing condition.
1) preparation of probiotics leaven
1. the preparation of bifidus bacillus
Frozen Bifidobacteria powder is inoculated in MRS lactose medium, 37 DEG C, after cultivating 24h, meet the standard form of bifidus bacillus through mirror inspection, growth conditions is good, is inoculated in MRS lactose medium by inoculum size 3%��6% and activates, 37 DEG C, cultivate 18��24h and save backup.
2. the preparation of Kefir fermenting agent
Use skimmed milk powder to be made into the skimming milk solution of 10%, through 90��115 DEG C, after 10��30min sterilization, it is cooled to 20��30 DEG C, according to 0.1%��0.5% inoculation Kefir granule of skimming milk volume, save backup after the 14��18h that ferments when 23��28 DEG C.
2) mixed fermentation
Get condensed whey protein 10��20g, glucose 6��10g, yeast powder 3��5g, calcium carbonate 1��5g, magnesium sulfate 0.05��0.3g, manganous sulfate 0.01��0.05g, whey powder 5��15g, inulin 2.5��5g, panaxan 0.5��5g, soybean oligosaccharide 0.5��5g, adding distilled water to total mass is 500g. Regulating medium pH to be 6.5��7.5 after fully dissolving, sterilization conditions is be incubated 10��30min under 90��115 DEG C of conditions, is cooled to 20��30 DEG C for subsequent use.
Culture medium inoculated after cooling, by 3%��10% inoculum size, Kefir fermenting agent bifidus bacillus=1��3 1,28��36 DEG C, fermentation 18��24h, obtains probiotic mixed fermentation liquid.
3) preparation of bacterium powder
Being stirred evenly by obtained fermented liquid, under 10��20 DEG C of conditions, rotating speed is 3000��6000 revs/min, and the time is 10��30min centrifugal treating, collects concentrated thalline; The thalline of centrifugal collection, skimmed milk powder 10%��15%, trehalose 1%��5%, Sodium Glutamate 1%��5%, glycerine 1%��3% is added by fermentating liquid volume, mix, carry out frozen drying after pre-freeze or it is 80��120 DEG C in inlet temperature, when air outlet temperature 50��70 DEG C, carry out low temperature spray drying, obtain probiotics bacterial powder.
4) compressing tablet
According to mass percent by 90%��98% probiotics bacterial powder, 1%��9% oligofructose, 0.1%��1% Magnesium Stearate mixes, and then utilizes tabletting machine, it is 4000��10000N condition lower sheeting at pressure, makes the composite probiotics micro-ecological tablet that unit viable count is greater than 6,000,000,000 cfu/g. Wherein, the diameter of tablet is 6��12mm, sheet weight: 0.3��0.5g, hardness: 8��10kg/cm2��
Claims (6)
1. a preparation method for probiotics micro-ecological tablet, described probiotics micro-ecological tablet, consists of by mass percentage, probiotics bacterial powder 90%��98%, oligofructose 1%��9%, Magnesium Stearate 0.1%��1%; First MRS lactose medium is used to be activated by bifidus bacillus, it may also be useful to skimming milk inoculation Kefir granule prepares Kefir fermenting agent; It is characterized in that, then obtain through mixed fermentation, cryoconcentration drying, batch mixing compressing tablet;
Described mixed fermentation prepares substratum by following process, and substratum component by weight percentage has, condensed whey protein 2%��10%, glucose 1%��10%, yeast powder 0.5%��1%, calcium carbonate 0.4%��1%, magnesium sulfate 0.01%��0.06%, manganous sulfate 0.001%��0.01%, whey powder 1%��10%, inulin 0.5%��3%, panaxan 0.04%��1%, soybean oligosaccharide 0.02%��1%, and surplus is distilled water; By each components dissolved in distilled water, regulate pH to be 6.5��7.5, under 90��115 DEG C of conditions, it is incubated 10��30min sterilization; It is cooled to 20��30 DEG C of mixed strainss by Kefir fermenting agent and bifidus bacillus to inoculate by the 3%��10% of culture volume, under 28��36 DEG C of conditions, ferment 16��24 hours, obtaining probiotic mixed fermentation liquid, wherein the inoculative proportion of Kefir fermenting agent and bifidus bacillus is Kefir fermenting agent: bifidus bacillus=1��3:1;
Described cryoconcentration is dry, be by probiotic mixed fermentation liquid when 3000��6000r/min, stir centrifugal 15��30min, collect concentrated thalline; In concentrated thalline, add protective material again, carry out vacuum lyophilization after pre-freeze or be 80��120 DEG C in inlet temperature, when air outlet temperature 50��70 DEG C, carry out low temperature spray drying, obtain probiotics bacterial powder;
Described batch mixing compressing tablet 90%��98% probiotics bacterial powder, 1%��9% oligofructose, 0.1%��1% Magnesium Stearate is mixed according to mass percent, and probiotics micro-ecological tablet made by compressing tablet.
2. according to the preparation method of probiotics micro-ecological tablet according to claim 1, it is characterized in that, described bifidus bacillus activates, it is that frozen Bifidobacteria powder is inoculated in MRS lactose medium, after 37 DEG C of cultivation 24h, meeting the standard form of bifidus bacillus through mirror inspection, growth conditions is good, it is inoculated in MRS lactose medium by inoculum size 3%��6% again to activate, cultivates 18��24h for 37 DEG C.
3. according to the preparation method of probiotics micro-ecological tablet according to claim 2, it is characterised in that, described Bifidobacteria powder is that bifidobacteria infantis powder, bifidus longum bb powder, bifidobacterium breve powder are or/and bifidobacterium adolescentis powder; Use 2��3 kinds of bacterium powder time, respectively activate remix obtain activation bifidus bacillus, for Kefir fermenting agent mixed fermentation.
4. according to the preparation method of probiotics micro-ecological tablet according to claim 1, it is characterized in that, described prepares Kefir fermenting agent, it is the skimming milk solution using skimmed milk powder to be made into 10%, through 90��115 DEG C, after 10��30min sterilization, being cooled to 20��30 DEG C, according to 0.1%��0.5% inoculation Kefir granule of skimming milk volume, ferment 14��18h when 23��28 DEG C.
5. according to the preparation method of the probiotics micro-ecological tablet described in claim 1,2 or 4; it is characterized in that; in cryoconcentration drying process; described protective material; it is be radix by probiotic mixed fermentation liquid quality, it is made up of skimmed milk powder 10%��20%, trehalose 1%��10%, Sodium Glutamate 1%��10%, glycerine 1%��5%.
6. according to the preparation method of the probiotics micro-ecological tablet described in claim 1,2 or 4, it is characterised in that, in batch mixing tableting processes, described compressing tablet carries out when pressure is 4000��10000N.
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| CN104904859B (en) * | 2015-04-21 | 2018-07-31 | 华南农业大学 | A kind of Sourbean milk fits property leavening processed and its preparation method and application |
| CN106579457A (en) * | 2016-12-30 | 2017-04-26 | 天津替代医学科技股份有限公司 | Butyric acid bacterium probiotic acid slice and preparation method thereof |
| CN108523133A (en) * | 2018-03-28 | 2018-09-14 | 广州富诺营养科技有限公司 | A kind of probiotics composite particles and preparation method thereof |
| CN111214524A (en) * | 2020-03-18 | 2020-06-02 | 北京中医药大学 | Application of ginseng fermented product |
| CN111956676A (en) * | 2020-08-24 | 2020-11-20 | 天津现代创新中药科技有限公司 | A microecological preparation containing Ginseng radix extract and probiotic bacteria, and its preparation method and application |
| CN112535214A (en) * | 2020-12-03 | 2021-03-23 | 石家庄君乐宝乳业有限公司 | Probiotic milk tablet with intestine regulating effect and preparation method thereof |
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