CN103843653A - High-quality high-yield seedling culture method of undaria pinnatifida - Google Patents
High-quality high-yield seedling culture method of undaria pinnatifida Download PDFInfo
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- CN103843653A CN103843653A CN201410103570.3A CN201410103570A CN103843653A CN 103843653 A CN103843653 A CN 103843653A CN 201410103570 A CN201410103570 A CN 201410103570A CN 103843653 A CN103843653 A CN 103843653A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to a high-quality high-yield seedling culture method of undaria pinnatifida. The high-quality high-yield seedling culture method comprises the following steps of 1, attachment substrate manufacturing and pretreatment; 2, cleaning: collected undaria pinnatifida main weeds are sufficiently washed in seawater for clearing impurity algae and other attachments attached on algae bodies; 3, seedling culture; 4, attachment substrate fixation: the attachment substrates with attached seedlings are conveyed to a cultivation sea region and are fixed on lithoherm of an intertidal zone, after 20 to 30 minutes, seawater is splashed and poured for 3 times every 5 minutes; 5, intertidal zone cultivation: the cultivation is carried out after cement lumps with attached seedlings are fixed, the seedlings are regularly checked, and harmful organisms are cleared.
Description
Technical field
The present invention relates to marine alga implantation methods, relate in particular to a kind of undaria pinnitafida good quality and high output seedling-cultivating method.
Background technology
Undaria pinnitafida is called extra large leaf mustard, opotism, and it belongs to a genus of Laminariales, and this genus is total! Kind, all marine products, are mainly distributed in Japan, Korea, and China only has a kind of undaria pinnitafida.Undaria pinnitafida is the edible seaweed that a kind of economic worth is higher, contains the trace elements such as abundant alginic acid, food fiber, physiological activator and iodine, potassium, calcium, and the intelligence development to children, women's beauty treatment, care of aged have special efficacy.Long-term edible undaria pinnitafida has certain treatment reason effect to prevention diabetes, angiocardiopathy, goitre, cancer etc.Undaria pinnitafida, because its taste is agreeable to the taste, nutritious, is liked by Japan and the Southeast Asia people deeply for a long time.The undaria pinnitafida aquaculture of the states such as Japan has formed certain scale, and China coast one is also flourish with the cultivation of undaria pinnitafida.
Summary of the invention
The technical problem to be solved in the present invention is: the invention provides a kind of undaria pinnitafida good quality and high output seedling-cultivating method, comprising the steps: 1) attaching substratum makes and pretreatment: the attaching substratum made from concrete, is accompanied by scallop shell on the surface of attaching substratum or is accompanied by sand and form coarse surface; By attaching substratum sea water immersion, then complete pretreatment with freshwater soaking;
2) undaria pinnitafida of collection is planted vegetables and in seawater, fully clean, remove assorted algae and other attachments of on frond, adhering to, concentrate at 6: 1 according to the male and female ratio that plants vegetables, gather the fertilized egg of diffusing, fertilized egg is adhered to density and is reached the 30-40/visual field (10 × 10), can stop collecting seedling;
3) seedling culture: the attaching substratum of handling well is laid on and is collected seedling in pond, by the fertilized egg artificially collecting, splash on attaching substratum, cultivate 20-30d under first stage, maintenance dark, sealing anoxic, 4 DEG C of conditions of temperature; Second stage, maintenance illumination 4500-5000lx, L: D=1: 1, under aerobic, 10 DEG C of conditions of temperature, cultivate 5-7d; Phase III, maintenance illumination 2000-2500lx, L: D=1: 1, under aerobic, 15 DEG C of conditions of temperature, cultivate 7-10d, keeping cultivating N concentration in water body is 4ppm, and P concentration is 0.4ppm; When seedling reaches 10mm and carry out above the artificial cultivation of intertidal zone; Wherein first the adherance that adheres to seedling is transported to mariculture area after 4 degree are placed 1-3 days again, can effectively improve like this adhesive rate of seedling;
4) attaching substratum is fixed: the attaching substratum that adheres to seedling is transported to mariculture area, be fixed on the cay of intertidal zone, and after 20-30min, the seawater 3 times of splashing, every minor tick 5min;
5) Mesolittoral zone culturing: the cement that adheres to seedling cultivates after sticking together and fixing, and makes regular check on and clear up harmful organisms;
6) results and visual its growing state of processing undaria pinnitafida results, adopt whole harvesting or interval harvesting: the more neat undaria pinnitafida of a rope is taked once to gather, and not of uniform size, have again and continue receiving greatly of the condition that keeps hanging and stay, when observing its base portion both sides and growing a certain size sporophyll, can gather in, receive too early, though frond is fresh and tender, frond is little, Ratio of dry to fresh matter of leaves is large, spend evening and receive, though Ratio of dry to fresh matter of leaves is little, output is high, but frond is aging, do not meet export standard.
The making of described adherance comprises the following steps: the selection of shape: truncated cone-shaped concrete adherance or trapezoidal cube concrete block are all suitable for undaria pinnitafida and increase and move in the process of growing juvenile sporophyte or survive adhering to of seedling; Circular diameter 20cm on truncated cone-shaped concrete adherance, lower circular diameter 40cm, high 40cm, weighs 60～75Kg, is a side groove at each quarter at 24cm, 30cm and 36cm place at round platform mid diameter, is suitable for the binding of diameter 0.4～0.8cm seedling rope; Wide 15 × 15cm on trapezoidal cube concrete block, lower wide 20 × 20cm, high 15cm;
Described attaching substratum fixed form comprises: method one: the truncated cone-shaped concrete adherance or the trapezoidal cube concrete block that build as artificial algal reef with notched truncated cone-shaped concrete, throw in the intertidal zone that is suitable for undaria pinnitafida growth; Utilize the undaria pinnitafida juvenile sporophyte of artificial sexual propagation cultivation as seedling source, be bundled in truncated cone-shaped concrete adherance groove every 10cm folder seedling with diameter 6～8mm nylon rope, and rhizoid is close in the groove of round platform, rhizoid firmly invests gradually on round platform in process of growth; Method two: the trapezoidal cube concrete block of suitable size, in indoor process immersion treatment, alkaline matter or noxious material are overflowed, in indoor raising of seedling workshop, adopt hydrostatic to adopt attached undaria pinnitafida juvenile sporophyte, indoor semi-fluid water planting was educated after 1～2 month, in the time of the long 2mm of juvenile sporophyte average body, be fixed on undaria pinnitafida ecosystem rock in the time of low water with accelerated cement.
Embodiment
A kind of undaria pinnitafida good quality and high output seedling-cultivating method, comprises the steps:
1) attaching substratum is made and pretreatment: the attaching substratum made from concrete, is accompanied by scallop shell on the surface of attaching substratum or is accompanied by sand and form coarse surface; By attaching substratum sea water immersion, then complete pretreatment with freshwater soaking;
2) undaria pinnitafida of collection is planted vegetables and in seawater, fully clean, remove assorted algae and other attachments of on frond, adhering to, concentrate at 6: 1 according to the male and female ratio that plants vegetables, gather the fertilized egg of diffusing, fertilized egg is adhered to density and is reached the 30-40/visual field (10 × 10), can stop collecting seedling;
3) seedling culture: the attaching substratum of handling well is laid on and is collected seedling in pond, by the fertilized egg artificially collecting, splash on attaching substratum, cultivate 20-30d under first stage, maintenance dark, sealing anoxic, 4 DEG C of conditions of temperature; Second stage, maintenance illumination 4500-5000lx, L: D=1: 1, under aerobic, 10 DEG C of conditions of temperature, cultivate 5-7d; Phase III, maintenance illumination 2000-2500lx, L: D=1: 1, under aerobic, 15 DEG C of conditions of temperature, cultivate 7-10d, keeping cultivating N concentration in water body is 4ppm, and P concentration is 0.4ppm; When seedling reaches 10mm and carry out above the artificial cultivation of intertidal zone; Wherein first the adherance that adheres to seedling is transported to mariculture area after 4 degree are placed 1-3 days again, can effectively improve like this adhesive rate of seedling;
4) attaching substratum is fixed: the attaching substratum that adheres to seedling is transported to mariculture area, be fixed on the cay of intertidal zone, and after 20-30min, the seawater 3 times of splashing, every minor tick 5min;
5) Mesolittoral zone culturing: the cement that adheres to seedling cultivates after sticking together and fixing, and makes regular check on and clear up harmful organisms;
6) results and visual its growing state of processing undaria pinnitafida results, adopt whole harvesting or interval harvesting: the more neat undaria pinnitafida of a rope is taked once to gather, and not of uniform size, have again and continue receiving greatly of the condition that keeps hanging and stay, when observing its base portion both sides and growing a certain size sporophyll, can gather in, receive too early, though frond is fresh and tender, frond is little, Ratio of dry to fresh matter of leaves is large, spend evening and receive, though Ratio of dry to fresh matter of leaves is little, output is high, but frond is aging, do not meet export standard.
The making of described adherance comprises the following steps: the selection of shape: truncated cone-shaped concrete adherance or trapezoidal cube concrete block are all suitable for undaria pinnitafida and increase and move in the process of growing juvenile sporophyte or survive adhering to of seedling; Circular diameter 20cm on truncated cone-shaped concrete adherance, lower circular diameter 40cm, high 40cm, weighs 60～75Kg, is a side groove at each quarter at 24cm, 30cm and 36cm place at round platform mid diameter, is suitable for the binding of diameter 0.4～0.8cm seedling rope; Wide 15 × 15cm on trapezoidal cube concrete block, lower wide 20 × 20cm, high 15cm;
Described attaching substratum fixed form comprises: method one: the truncated cone-shaped concrete adherance or the trapezoidal cube concrete block that build as artificial algal reef with notched truncated cone-shaped concrete, throw in the intertidal zone that is suitable for undaria pinnitafida growth; Utilize the undaria pinnitafida juvenile sporophyte of artificial sexual propagation cultivation as seedling source, be bundled in truncated cone-shaped concrete adherance groove every 10cm folder seedling with diameter 6～8mm nylon rope, and rhizoid is close in the groove of round platform, rhizoid firmly invests gradually on round platform in process of growth; Method two: the trapezoidal cube concrete block of suitable size, in indoor process immersion treatment, alkaline matter or noxious material are overflowed, in indoor raising of seedling workshop, adopt hydrostatic to adopt attached undaria pinnitafida juvenile sporophyte, indoor semi-fluid water planting was educated after 1～2 month, in the time of the long 2mm of juvenile sporophyte average body, be fixed on undaria pinnitafida ecosystem rock in the time of low water with accelerated cement.
The present embodiment is 6～July in 2012, and on black carp beach, Rongcheng, nursery carries out, and 700 of training breeding screens, obtain following result: seedling averag density 20.3 strains/cm in the indoor culture cycle of 15 days
2, the average spatfall number 16.24 ten thousand strains/curtains of seedling curtain, spatfall rate reaches more than 80%, average height of seedling 1.6mm, and the seed of cultivating is even, healthy and strong neat.And traditional seedling-cultivating method, seedling averag density 12 strains/cm in the indoor culture cycle of 15 days
2, the average spatfall number of seedling curtain: 9.6 ten thousand strains/curtain, spatfall rate 40%～60%, average height of seedling 1～1.2mm, if will reach the height of seedling of the present embodiment, will lengthen the cultivation period of 5～10 days.
Claims (3)
1. a undaria pinnitafida good quality and high output seedling-cultivating method, comprises the steps:
1) attaching substratum is made and pretreatment: the attaching substratum made from concrete, is accompanied by scallop shell on the surface of attaching substratum or is accompanied by sand and form coarse surface; By attaching substratum sea water immersion, then complete pretreatment with freshwater soaking;
2) undaria pinnitafida of collection is planted vegetables and in seawater, fully clean, remove assorted algae and other attachments of on frond, adhering to, concentrate at 6: 1 according to the male and female ratio that plants vegetables, gather the fertilized egg of diffusing, fertilized egg is adhered to density and is reached the 30-40/visual field, can stop collecting seedling;
3) seedling culture: the attaching substratum of handling well is laid on and is collected seedling in pond, by the fertilized egg artificially collecting, splash on attaching substratum, cultivate 20-30d under first stage, maintenance dark, sealing anoxic, 4 DEG C of conditions of temperature; Second stage, maintenance illumination 4500-5000lx, L: D=1: 1, under aerobic, 10 DEG C of conditions of temperature, cultivate 5-7d; Phase III, maintenance illumination 2000-2500lx, L: D=1: 1, under aerobic, 15 DEG C of conditions of temperature, cultivate 7-10d, keeping cultivating N concentration in water body is 4ppm, and P concentration is 0.4ppm; When seedling reaches 10mm and carry out above the artificial cultivation of intertidal zone; Wherein first the adherance that adheres to seedling is transported to mariculture area after 4 degree are placed 1-3 days again, can effectively improve like this adhesive rate of seedling;
4) attaching substratum is fixed: the attaching substratum that adheres to seedling is transported to mariculture area, be fixed on the cay of intertidal zone, and after 20-30min, the seawater 3 times of splashing, every minor tick 5min
5) Mesolittoral zone culturing: the cement that adheres to seedling cultivates after sticking together and fixing, and makes regular check on and clear up harmful organisms;
6) results and visual its growing state of processing undaria pinnitafida results, adopt whole harvesting or interval harvesting: the more neat undaria pinnitafida of a rope is taked once to gather, and not of uniform size, have again and continue receiving greatly of the condition that keeps hanging and stay, when observing its base portion both sides and growing a certain size sporophyll, can gather in, receive too early, though frond is fresh and tender, frond is little, Ratio of dry to fresh matter of leaves is large, spend evening and receive, though Ratio of dry to fresh matter of leaves is little, output is high, but frond is aging, do not meet export standard.
2. undaria pinnitafida good quality and high output seedling-cultivating method as claimed in claim 1, is characterized in that: the making of described adherance comprises the following steps: the selection of shape: truncated cone-shaped concrete adherance or trapezoidal cube concrete block are all suitable for undaria pinnitafida and increase and move in the process of growing juvenile sporophyte or survive adhering to of seedling; Circular diameter 20cm on truncated cone-shaped concrete adherance, lower circular diameter 40cm, high 40cm, weighs 60～75Kg, is a side groove at each quarter at 24cm, 30cm and 36cm place at round platform mid diameter, is suitable for the binding of diameter 0.4～0.8cm seedling rope; Wide 15 × 15cm on trapezoidal cube concrete block, lower wide 20 × 20cm, high 15cm.
3. undaria pinnitafida good quality and high output seedling-cultivating method as claimed in claim 1, it is characterized in that: described attaching substratum fixed form comprises: method one: the truncated cone-shaped concrete adherance or the trapezoidal cube concrete block that build as artificial algal reef with notched truncated cone-shaped concrete, throw in the intertidal zone that is suitable for undaria pinnitafida growth; Utilize the undaria pinnitafida juvenile sporophyte of artificial sexual propagation cultivation as seedling source, be bundled in truncated cone-shaped concrete adherance groove every 10cm folder seedling with diameter 6～8mm nylon rope, and rhizoid is close in the groove of round platform, rhizoid firmly invests gradually on round platform in process of growth;
Method two: the trapezoidal cube concrete block of suitable size, in indoor process immersion treatment, alkaline matter or noxious material are overflowed, in indoor raising of seedling workshop, adopt hydrostatic to adopt attached undaria pinnitafida juvenile sporophyte, indoor semi-fluid water planting was educated after 1～2 month, in the time of the long 2mm of juvenile sporophyte average body, be fixed on undaria pinnitafida ecosystem rock in the time of low water with accelerated cement.
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Cited By (7)
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| CN105706893A (en) * | 2016-02-03 | 2016-06-29 | 大连海宝渔业有限公司 | Cloned undaria pinnatifida seedling attachment method |
| CN107750937A (en) * | 2017-10-24 | 2018-03-06 | 山东省海洋生物研究院 | A kind of attachment base suitable for Porphyra yezoensis conchocelis breeding prepares and its application method |
| CN107950380A (en) * | 2017-12-21 | 2018-04-24 | 山东省农业科学院生物技术研究中心 | A kind of cultivation of undaria pinnitafida and the method for administering red tide |
| CN109105248A (en) * | 2018-08-29 | 2019-01-01 | 湖北工业大学 | A kind of indoor germination method of stonewort brood body |
| CN109220768A (en) * | 2018-10-25 | 2019-01-18 | 青岛农业大学 | A kind of mussel attachment base kelp seedling intermediate breeding method |
| CN109566395A (en) * | 2019-01-15 | 2019-04-05 | 浙江省海洋水产养殖研究所 | Littoral zone ecological system degradation method |
| CN109964805A (en) * | 2019-05-09 | 2019-07-05 | 大连海宝渔业有限公司 | A method of benthic diatom on processing Undaria pinnitafida seedling curtain |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706893A (en) * | 2016-02-03 | 2016-06-29 | 大连海宝渔业有限公司 | Cloned undaria pinnatifida seedling attachment method |
| CN107750937A (en) * | 2017-10-24 | 2018-03-06 | 山东省海洋生物研究院 | A kind of attachment base suitable for Porphyra yezoensis conchocelis breeding prepares and its application method |
| CN107950380A (en) * | 2017-12-21 | 2018-04-24 | 山东省农业科学院生物技术研究中心 | A kind of cultivation of undaria pinnitafida and the method for administering red tide |
| CN109105248A (en) * | 2018-08-29 | 2019-01-01 | 湖北工业大学 | A kind of indoor germination method of stonewort brood body |
| CN109220768A (en) * | 2018-10-25 | 2019-01-18 | 青岛农业大学 | A kind of mussel attachment base kelp seedling intermediate breeding method |
| CN109566395A (en) * | 2019-01-15 | 2019-04-05 | 浙江省海洋水产养殖研究所 | Littoral zone ecological system degradation method |
| CN109964805A (en) * | 2019-05-09 | 2019-07-05 | 大连海宝渔业有限公司 | A method of benthic diatom on processing Undaria pinnitafida seedling curtain |
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