CN103834600A - 一种催化合成丙烯酰胺的光敏性腈水合酶菌株的发酵方法 - Google Patents
一种催化合成丙烯酰胺的光敏性腈水合酶菌株的发酵方法 Download PDFInfo
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- CN103834600A CN103834600A CN201410096647.9A CN201410096647A CN103834600A CN 103834600 A CN103834600 A CN 103834600A CN 201410096647 A CN201410096647 A CN 201410096647A CN 103834600 A CN103834600 A CN 103834600A
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- acrylamide
- nitrile hydratase
- strain
- fermentation
- bacterial strain
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- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 108010024026 Nitrile hydratase Proteins 0.000 title claims abstract description 36
- 238000000855 fermentation Methods 0.000 title claims abstract description 22
- 230000004151 fermentation Effects 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title abstract description 34
- 230000001580 bacterial effect Effects 0.000 title abstract description 20
- 230000015572 biosynthetic process Effects 0.000 title abstract description 8
- 238000003786 synthesis reaction Methods 0.000 title abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 241000589776 Pseudomonas putida Species 0.000 claims abstract description 11
- 238000005286 illumination Methods 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 5
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 206010034972 Photosensitivity reaction Diseases 0.000 claims description 9
- 230000036211 photosensitivity Effects 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- 239000004202 carbamide Substances 0.000 claims description 6
- 229960002989 glutamic acid Drugs 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 3
- 239000000047 product Substances 0.000 abstract description 14
- 238000006703 hydration reaction Methods 0.000 abstract description 10
- 230000036571 hydration Effects 0.000 abstract description 9
- 238000006555 catalytic reaction Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 230000003213 activating effect Effects 0.000 abstract 1
- 239000006227 byproduct Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 17
- 239000003054 catalyst Substances 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 12
- 238000004519 manufacturing process Methods 0.000 description 12
- 239000000725 suspension Substances 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 9
- 150000002825 nitriles Chemical class 0.000 description 8
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 7
- 229910052622 kaolinite Inorganic materials 0.000 description 7
- 230000009466 transformation Effects 0.000 description 7
- 239000002028 Biomass Substances 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 238000005303 weighing Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 229920002401 polyacrylamide Polymers 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 231100000219 mutagenic Toxicity 0.000 description 3
- 230000003505 mutagenic effect Effects 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- -1 nitrile compound Chemical class 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 101100412856 Mus musculus Rhod gene Proteins 0.000 description 1
- 241000190932 Rhodopseudomonas Species 0.000 description 1
- 101100242191 Tetraodon nigroviridis rho gene Proteins 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 238000007603 infrared drying Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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Abstract
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CN201410096647.9A CN103834600A (zh) | 2014-03-17 | 2014-03-17 | 一种催化合成丙烯酰胺的光敏性腈水合酶菌株的发酵方法 |
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CN201410096647.9A CN103834600A (zh) | 2014-03-17 | 2014-03-17 | 一种催化合成丙烯酰胺的光敏性腈水合酶菌株的发酵方法 |
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CN201410096647.9A Pending CN103834600A (zh) | 2014-03-17 | 2014-03-17 | 一种催化合成丙烯酰胺的光敏性腈水合酶菌株的发酵方法 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115216465A (zh) * | 2022-08-15 | 2022-10-21 | 武汉宏择一碳环保科技有限公司 | 一种基于膜分离工艺的丙烯酰胺制备方法 |
CN115558684A (zh) * | 2022-09-27 | 2023-01-03 | 南通博亿化工有限公司 | 一种酰胺化合物的生物制备方法以及生物法制备的丙烯酰胺 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102329757A (zh) * | 2011-10-10 | 2012-01-25 | 孙安顺 | 一种用于合成丙烯酰胺含有光敏性腈水合酶菌株的催化剂及其应用 |
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2014
- 2014-03-17 CN CN201410096647.9A patent/CN103834600A/zh active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102329757A (zh) * | 2011-10-10 | 2012-01-25 | 孙安顺 | 一种用于合成丙烯酰胺含有光敏性腈水合酶菌株的催化剂及其应用 |
Non-Patent Citations (3)
Title |
---|
孙韦强: "利用微生物转化丙烯腈成丙烯酰胺的研究_产腈水合酶菌种的发酵条件的研究", 《石油学报(石油加工)》 * |
孙韦强: "恶臭假单胞菌整细胞的腈水合酶催化特性", 《分子催化》 * |
张刚等: "恶臭假单胞菌JP_1产腈水合酶的发酵工艺研究", 《生物工程学报》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115216465A (zh) * | 2022-08-15 | 2022-10-21 | 武汉宏择一碳环保科技有限公司 | 一种基于膜分离工艺的丙烯酰胺制备方法 |
CN115558684A (zh) * | 2022-09-27 | 2023-01-03 | 南通博亿化工有限公司 | 一种酰胺化合物的生物制备方法以及生物法制备的丙烯酰胺 |
CN115558684B (zh) * | 2022-09-27 | 2023-08-18 | 南通博亿化工有限公司 | 一种酰胺化合物的生物制备方法以及生物法制备的丙烯酰胺 |
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Application publication date: 20140604 |