CN103798130A - Watermelon hybrid seed production method - Google Patents
Watermelon hybrid seed production method Download PDFInfo
- Publication number
- CN103798130A CN103798130A CN201410047314.7A CN201410047314A CN103798130A CN 103798130 A CN103798130 A CN 103798130A CN 201410047314 A CN201410047314 A CN 201410047314A CN 103798130 A CN103798130 A CN 103798130A
- Authority
- CN
- China
- Prior art keywords
- seed
- melon
- pollination
- maternal
- male
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a watermelon hybrid seed production method which comprises the following steps: (1) seed production site and soil selection; (2) parent purification; (3) seed sowing; (4) culture management; (5) hybrid pollination; and (6) collection. According to the method, female parent castration, female flower double bagging, rear 5-6-section topping uniform female parent pollinated melons and the like are performed in advance, thereby effectively preventing hybridization and enhancing the hybrid seed production purity and hybrid seed production efficiency.
Description
Technical field
The present invention relates to a kind of crops cross-breeding method, especially relate to a kind of production of watermelon hybrid seeds method.
Background technology
Watermelon is one of important in the world fruit, and the area under cultivation of China's watermelon ranks first in the world, and watermelon is produced promoting rural economic development, changes crop mix and raising living standards of urban and rural residents level etc. and all has positive effect.The watermelon of China is produced with breeding research work and is started late, in existing production of watermelon hybrid seeds process, seldom carry out female parent and shift to an earlier date maternal two baggings before and after continuous emasculation, hybridization pollination, the female parent disconnected point of 5～6 joint operation sequence such as pinch of being pollinated after melon, or only carry out part operation program, hybridization purity is undesirable, hybrid seed yield is low, causes the output of watermelon, quality greatly to reduce.
Summary of the invention
The technical problem to be solved in the present invention is, overcomes the deficiencies in the prior art, and the production of watermelon hybrid seeds that a kind of hybridization seed production purity is high, efficiency is high method is provided.
The technical scheme that the present invention solves its technical problem employing is that production of watermelon hybrid seeds method, comprises the following steps:
(1) select production of hybrid seeds place and soil
Selection precipitation rareness, drought, subterranean water level is low, and the dry land of photo-thermal repository (such as NORTHWEST CHINA one with Xinjiang region, Gansu Province etc.), as production of watermelon hybrid seeds ground;
Energy drought-relief, waterlogging-preventing should be selected in the melon patch on production of hybrid seeds ground, and 6-7 does not plant the field of watermelon, more than isolating 1000m between kind;
(2) parent's purifying
Parent's (like can No. 5 watermelon male parent XL10, liking can No. 5 maternal XM12 of watermelons) must strictly isolate breeding according to varietal characteristic separately, selfing pure keeping, select the interior seed of homophyletic selfing standard list melon as breeding man seed, with the seed isolation breeding of breeding man, as original seed, original seed mixes collects seed as production of hybrid seeds parental seed;
(3) planting density and sowing
Love through seed selection can No. 5 maternal XM12 thickness of sowing 2500-2700 strain/667m
2, generally adopt lively, determine plastic mulching according to the production of hybrid seeds ground weather conditions sowing date; Love through seed selection can No. 5 more maternal thin plantings, the 2000-2450 strain/667m of omiting of male parent XL10
2, sowing in 7～10 days in advance, guarantees both flower synchronizations, two films cover; Plant ratio male parent: female parent is 1: 18-22;
(4) cultivation management
Use sufficient base manure, take composite fertilizer, phosphorus, potash fertilizer, farm organic fertilizer as main, determine amount of application according to the fertile degree in soil, in order to strong seed and raising grain yield, pollinated stage imposes foliage fertilizer and promotes setting, improves seed production and quality;
Pour water according to kind leaf-shrinkage degree, guarantee that plant normally nourishes and grows; Maternal single climing training, pinches at the disconnected point of 5～6 joint after melon that is pollinated after pollination, in order to producing Crossbred, seat melon, and guarantees purity to improve hybrid seed yield; The climing training of male parent 3～4 or do not prune, applies fertilizer more, topdresses, guarantee that the open quantity of male flower is many, it is sturdy to spend, pollen enough;
Note the extermination of disease and insect pest, spray medicament kill insects at pollinated stage, reduce insect string powder, guarantee seed production purity;
(5) hybridization pollination
5.1 paper caps, label are prepared: 3000～4000, the paper cap of every mu of preparation thumbnail size, and label generally adopts knitting wool, safety pin, paint, cordage etc.;
5.2 male parent impurity eliminations: close inspection male parent purity before pollination, strictly select good strains in the field for seed according to the feature of male parent (leaf, leaf look, ovary shape etc.), removing special-shaped different strain etc. has doubt plant;
5.3 emasculations: pollinate the previous day, thoroughly remove male flower and male flower bud on maternal plant, between pollination period, all will carry out every day, can not see open male flower in maternal strain while guaranteeing pollination; Male flower and the male flower bud removed can not be lost in the ground, and should take away or buried, because of its loose powder that can be open as usual for second day, and lose the meaning of emasculation;
5.4 pairs of baggings: pollination operates the maternal female flower paper using cap sleeve that will open the day before yesterday and lives, and after pollination in second day, paper using cap sleeve is lived again;
5.5 adopt male flower: pollination operation was plucked and will open or the male flower of open male parent strain morning on the same day, was loaded in wide-mouth vial, covered moistening towel; The same day, unspent male flower can not utilize in next day or pollination later, abandoned without exception; Under the weather condition of overcast and rainy, low temperature and high relative humidity, though adopting, male flower must notice that the male flower of having opened still closes up, paler colour, not bright-coloured, must not pluck again;
5.6 pollinations: be generally advisable with the second female flower pollination in pollination joint position; Maternal strain female flower started opening and can carry out morning, when temperature is high, dry, opened early, and temperature is low, open more lately when high humidity; Take off the previous day and femalely take overlapped paper cap, be coated in gently on the column cap of maternal female flower with the polliniferous male parent male flower of just having adopted, paternal pollen is evenly sticked on maternal column cap, afterwards, paper cap is continued to put; For improving pollination rate, can repeat to invest same female flower with 3-4 male flowers simultaneously, reusable 3～4 times of a general male flower, when male flower flower quantity not sufficient, number of times capable of reducing using; In maternal strain, there is the not young melon of pollination of omission to extract as early as possible, continue the female flower of pollination with deutomerite position;
5.7 mark: after pollination bagging, on the melon handle of the melon of pollinating or the climing mark of carrying out of melon of front and back joints;
(6) gather
6.1 impurity eliminations: after maternal melon fully grows up to (general 20 days), according to maternal feature, remove the most field melon of mixing;
6.2 gather: plant after the abundant maturation of melon, according to the pollination mark doing, after harvesting pollination melon, concentrate and stack, after-ripening 3～5 days, can cut open melon and collect seed; Cuing open in the process that melon collects seed, should observe seed size in each melon, color, crackle etc., the seed that does not meet variety characteristic is strictly eliminated;
6.3 bask seeds: after collecting seed, melon pulp should not ferment, and should, clean when daily clear water rinsing, remove float, white blighted grain, should use articles made of bamboo, woodenware to rinse airing, in order to avoid seed variable color; Forbidding metalware or on cement flooring, stone under high temperature high light airing seed, in order to avoid reduce percentage of seedgermination;
6.4 packs: cleanliness, the seed that water content is qualified perform label, pack, warehouse-in, note interracial differentiation placement therebetween, is strictly on guard against mechanical admixture, finally samples and identify, registered seed is for market sale.
The present invention carries out in advance after the two baggings of maternal emasculation, female flower, maternal pollination, in the operation that after melon, the disconnected point of 5～6 joint is pinched of being pollinated, can effectively preventing hybridization, can improve hybridization seed production purity and hybrid seeding efficiency.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1
The present embodiment comprises the following steps:
(1) select production of hybrid seeds place and soil
Yi Dai Xinjiang, northwest precipitation rareness, drought, photo-thermal aboundresources, as production of watermelon hybrid seeds ground; Be convenient to transportation, management, isolation, producing, ensure the quality of products and lower-cost prerequisite under determine concrete production of hybrid seeds place;
The melon patch on production of hybrid seeds ground selects subterranean water level low, and energy drought-relief, waterlogging-preventing, does not plant the field of watermelon in 6 years, isolates 1500m between kind;
(2) parent's purifying
Parent's (like can No. 5 watermelon male parent XL10, liking can No. 5 maternal XM12 of watermelons) strictly isolates breeding according to varietal characteristic separately, selfing pure keeping, select the interior seed of homophyletic selfing standard list melon as breeding man seed, with the seed isolation breeding of breeding man, as original seed, original seed mixes collects seed as production of hybrid seeds parental seed;
(3) planting density and sowing
Liking can No. 5 maternal XM12 thickness of sowing of watermelon 2500 strains/667m
2, adopt lively, determine plastic mulching according to the production of hybrid seeds ground weather conditions sowing date; Liking the more maternal slightly thin plantings of No. 5 watermelon male parent XL10 of energy, is 2200 strains/667m
2, sowing in 10 days in advance, guarantees both flower synchronizations, two films cover; Plant ratio male parent: female parent is 1: 20;
(4) cultivation management
Use sufficient base manure, take composite fertilizer, phosphorus, potash fertilizer, farm organic fertilizer as main, in order to strong seed and raising grain yield, pollinated stage repeatedly foliage spray can promote setting, improves seed production and quality;
Pour water according to kind Leaf shrinkage degree, guarantee that plant normally nourishes and grows; Maternal single climing training, pinches at the disconnected point of 5～6 joint after melon that is pollinated after pollination, is beneficial to producing Crossbred, seat melon, and guarantees purity to improve hybrid seed yield; The climing training of male parent 3～4 or do not prune, applies fertilizer more, topdresses, guarantee that the open quantity of male flower is many, it is sturdy to spend, pollen enough;
Note the extermination of disease and insect pest, spray medicament kill insects at pollinated stage, reduce insect string powder, guarantee seed production purity;
(5) hybridization pollination
5.1 paper caps, label are prepared: 3500, the paper cap of every mu of preparation thumbnail size, and label adopts safety pin;
5.2 male parent impurity eliminations: close inspection male parent purity before pollination, strictly select good strains in the field for seed according to the feature of male parent (leaf, leaf look, ovary shape etc.), removing special-shaped different strain etc. has doubt plant;
5.3 emasculations: pollinate the previous day, thoroughly remove male flower and male flower bud on maternal plant, between pollination period, all will carry out every day, can not see open male flower in maternal strain while guaranteeing pollination; Male flower and the male flower bud removed can not be lost in the ground, and should take away or buried, because of its loose powder that can be open as usual for second day, and lose the meaning of emasculation;
5.4 pairs of baggings: pollination operates the maternal female flower paper using cap sleeve that will open the day before yesterday and lives, and after pollination in second day, paper using cap sleeve is lived again;
5.5 adopt male flower: pollination operation was plucked and will open or the male flower of open male parent strain morning on the same day, was loaded in wide-mouth vial, covered moistening towel; The same day, unspent male flower can not utilize in next day or pollination later, abandoned without exception; Under the weather condition of overcast and rainy, low temperature and high relative humidity, though adopting, male flower must notice that the male flower of having opened still closes up, paler colour, not bright-coloured, must not pluck again;
5.6 pollinations: pollination joint position is with the second female flower pollination; Maternal strain female flower started to bloom and can carry out morning, when temperature is high, dry, opened early, and temperature is low, open more lately when high humidity; Take off the previous day and femalely take overlapped paper cap, be coated in gently on the column cap of maternal female flower with the polliniferous male parent male flower of just having adopted, paternal pollen is evenly sticked on maternal column cap, afterwards, paper cap is continued to put; For improving pollination rate, repeat to invest same female flower with 3 male flowers, reusable 3 times of male flower simultaneously; In maternal strain, there is the not young melon of pollination of omission to extract as early as possible, continue the female flower of pollination with deutomerite position;
5.7 mark: after pollination bagging, on the melon handle of the melon of pollinating or the climing mark of carrying out of melon of front and back joints;
(6) gather
6.1 impurity eliminations: after maternal melon fully grows up to (20 days), according to maternal feature, remove the most field melon of mixing;
6.2 gather: plant after the abundant maturation of melon, according to the pollination mark doing, after harvesting pollination melon, concentrate and stack, after-ripening 5 days, can cut open melon and collect seed; Cuing open in the process that melon collects seed, should observe seed size in each melon, color, crackle etc., the seed that does not meet variety characteristic is eliminated;
6.3 bask seeds: the rear melon pulp of collecting seed, clean when daily clear water rinsing, is removed float, white blighted grain, with articles made of bamboo, woodenware flushing airing, in order to avoid seed variable color; Forbidding metalware or on cement flooring, stone under high temperature high light airing seed, in order to avoid reduce percentage of seedgermination;
6.4 packs: cleanliness, the seed that water content is qualified perform label, pack, warehouse-in, note interracial differentiation placement therebetween, is strictly on guard against mechanical admixture, finally samples and identify, registered seed is for market sale.
The present embodiment is liked can No. 5 production of watermelon hybrid seeds purity 98 ﹪.
Claims (1)
1. production of watermelon hybrid seeds method, is characterized in that, comprises the following steps:
(1) select production of hybrid seeds place and soil
Selection precipitation rareness, drought, subterranean water level is low, and the dry land of photo-thermal repository, as production of watermelon hybrid seeds ground;
Energy drought-relief, waterlogging-preventing is selected in the melon patch on production of hybrid seeds ground, and 6-7 does not plant the field of watermelon, more than isolating 1000m between kind;
(2) parent's purifying
Parent: like No. 5 watermelon male parent XL10 of energy, like the maternal XM12 of No. 5 watermelons of energy, must strictly isolate breeding according to varietal characteristic separately, selfing pure keeping, select the interior seed of homophyletic selfing standard list melon as breeding man seed, with the seed isolation breeding of breeding man, as original seed, original seed mixes collects seed as production of hybrid seeds parental seed;
(3) planting density and sowing
Love through seed selection can No. 5 maternal XM12 thickness of sowing 2500-2700 strain/667m
2, adopt lively, determine plastic mulching according to the production of hybrid seeds ground weather conditions sowing date; Love through seed selection can No. 5 more maternal thin plantings, the 2000-2450 strain/667m of omiting of male parent XL10
2, sowing in 7～10 days in advance, guarantees both flower synchronizations, two films cover; Plant ratio male parent: female parent is 1: 18-22;
(4) cultivation management
Use sufficient base manure, take composite fertilizer, phosphorus, potash fertilizer, farm organic fertilizer as main, determine amount of application according to the fertile degree in soil, in order to strong seed and raising grain yield, pollinated stage imposes foliage fertilizer;
Pour water according to kind leaf-shrinkage degree, guarantee that plant normally nourishes and grows; Maternal single climing training, pinches at the disconnected point of 5～6 joint after melon that is pollinated after pollination, in order to producing Crossbred, seat melon, and guarantees purity to improve hybrid seed yield; The climing training of male parent 3～4 or do not prune, applies fertilizer more, topdresses, guarantee that the open quantity of male flower is many, it is sturdy to spend, pollen enough;
Note the extermination of disease and insect pest, spray medicament kill insects at pollinated stage, reduce insect string powder;
(5) hybridization pollination
5.1 paper caps, label are prepared: 3000～4000, the paper cap of every mu of preparation thumbnail size, and label adopts knitting wool, safety pin, paint or cordage;
5.2 male parent impurity eliminations: close inspection male parent purity before pollination, remove and have doubt plant;
5.3 emasculations: pollinate the previous day, thoroughly remove male flower and male flower bud on maternal plant, between pollination period, all will carry out every day, can not see open male flower in maternal strain while guaranteeing pollination; Male flower and the male flower bud removed can not be lost in the ground, and should take away or buried;
5.4 pairs of baggings: pollination operates the previous day, the maternal female flower paper using cap sleeve that will open is lived, and after pollination in second day, paper using cap sleeve is lived again;
5.5 adopt male flower: pollination operation was plucked and will open or the male flower of open male parent strain morning on the same day, was loaded in wide-mouth vial, covered moistening towel; The same day, unspent male flower can not utilize in next day or pollination later, abandoned without exception; Under the weather condition of overcast and rainy, low temperature and high relative humidity, though adopting, male flower must notice that the male flower of having opened still closes up, paler colour, not bright-coloured, can not pluck again;
5.6 pollinations: be advisable with the second female flower pollination in pollination joint position; Maternal strain female flower started opening and can carry out morning, when temperature is high, dry, opened early, and temperature is low, open more lately when high humidity; Take off the previous day and femalely take overlapped paper cap, be coated in gently on the column cap of maternal female flower with the polliniferous male parent male flower of just having adopted, paternal pollen is evenly sticked on maternal column cap, afterwards, paper cap is continued to put;
5.7 mark: after pollination bagging, on the melon handle of the melon of pollinating or the climing mark of carrying out of melon of front and back joints;
(6) gather
6.1 impurity eliminations: after maternal melon fully grows up to, according to maternal feature, remove the most field melon of mixing;
6.2 gather: plant after the abundant maturation of melon, according to the pollination mark doing, after harvesting pollination melon, concentrate and stack, after-ripening 3～5 days, cuts open melon and collect seed; Cuing open in the process that melon collects seed, should observe seed size in each kind of melon, color, crackle etc., the seed that does not meet variety characteristic is eliminated;
6.3 bask seeds: the rear melon pulp of collecting seed, clean when daily clear water rinsing, is removed float, white blighted grain, with articles made of bamboo, woodenware flushing airing, in order to avoid seed variable color;
6.4 packs: cleanliness, seed that water content is qualified are performed to label, pack, warehouse-in.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410047314.7A CN103798130A (en) | 2014-02-11 | 2014-02-11 | Watermelon hybrid seed production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410047314.7A CN103798130A (en) | 2014-02-11 | 2014-02-11 | Watermelon hybrid seed production method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103798130A true CN103798130A (en) | 2014-05-21 |
Family
ID=50695906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410047314.7A Pending CN103798130A (en) | 2014-02-11 | 2014-02-11 | Watermelon hybrid seed production method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103798130A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221654A (en) * | 2014-07-30 | 2014-12-24 | 镇江瑞繁农艺有限公司 | Method for increasing seed production amount of seedless watermelons |
| CN104885913A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | Labeling method for watermelon hybrid seed production |
| CN110447484A (en) * | 2019-08-20 | 2019-11-15 | 甘肃省农业科学院农业经济与信息研究所 | A kind of hybrid water melon seed purity identification method |
| CN113711867A (en) * | 2021-09-24 | 2021-11-30 | 宁波市农业科学研究院 | Method for improving seed production yield of wild watermelon |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1161130A (en) * | 1997-01-03 | 1997-10-08 | 吴德全 | Method for breeding seeds of hybridized water melon |
| CN1161133A (en) * | 1997-01-03 | 1997-10-08 | 吴德全 | Method for breeding seeds of early-maturing and large type hybridized water melon |
| CN103168677A (en) * | 2013-03-26 | 2013-06-26 | 天津科润农业科技股份有限公司蔬菜研究所 | Watermelon hybrid seed production method without artificial cross pollination |
| JP2013544518A (en) * | 2010-11-24 | 2013-12-19 | ヌンヘムス・ベー・ヴェー | Dual purpose pollinated watermelon |
-
2014
- 2014-02-11 CN CN201410047314.7A patent/CN103798130A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1161130A (en) * | 1997-01-03 | 1997-10-08 | 吴德全 | Method for breeding seeds of hybridized water melon |
| CN1161133A (en) * | 1997-01-03 | 1997-10-08 | 吴德全 | Method for breeding seeds of early-maturing and large type hybridized water melon |
| JP2013544518A (en) * | 2010-11-24 | 2013-12-19 | ヌンヘムス・ベー・ヴェー | Dual purpose pollinated watermelon |
| CN103168677A (en) * | 2013-03-26 | 2013-06-26 | 天津科润农业科技股份有限公司蔬菜研究所 | Watermelon hybrid seed production method without artificial cross pollination |
Non-Patent Citations (2)
| Title |
|---|
| 张贵山: "西瓜杂交制种技术操作要点", 《现代农业科技(下半月刊)》 * |
| 陶抵辉等: "西瓜新品种爱能5号的选育", 《中国蔬菜》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221654A (en) * | 2014-07-30 | 2014-12-24 | 镇江瑞繁农艺有限公司 | Method for increasing seed production amount of seedless watermelons |
| CN104885913A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | Labeling method for watermelon hybrid seed production |
| CN110447484A (en) * | 2019-08-20 | 2019-11-15 | 甘肃省农业科学院农业经济与信息研究所 | A kind of hybrid water melon seed purity identification method |
| CN110447484B (en) * | 2019-08-20 | 2021-04-27 | 甘肃省农业科学院农业经济与信息研究所 | Purity identification method for watermelon hybrid seeds |
| CN113711867A (en) * | 2021-09-24 | 2021-11-30 | 宁波市农业科学研究院 | Method for improving seed production yield of wild watermelon |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106258368A (en) | A kind of new method of the efficient sprouting and rooting of Fructus Fragariae Ananssae stolon | |
| CN102524053B (en) | Seed production method for hybrid pepper variety | |
| CN101663996B (en) | Method for high yield, prematurity and dwarfing cultivation of jatropha curcas | |
| CN101816282A (en) | Hybrid round eggplant seed production method | |
| CN103329802A (en) | Pollution-free high-yield planting method for strawberries | |
| CN103798130A (en) | Watermelon hybrid seed production method | |
| CN103798129A (en) | Watermelon hybrid seed production method | |
| CN104303731A (en) | Zucchini breed hybrid seed production method | |
| CN107172976B (en) | Improved sugarcane hybrid seed production method | |
| CN106258506A (en) | A kind of method of pearl guava graft seedling growth | |
| CN101647388A (en) | Method for breeding tidal marsh spartina, sweet sorghum, rice hybrid feed and energy resource crop | |
| CN105123496A (en) | Paddy rice hybridization method | |
| CN108157045A (en) | A kind of mulberry tree interplanting coprinus comatus and its cultural method of bacteria residue direct returning to farmland | |
| CN100411507C (en) | Paddy rice hybridizing method | |
| CN104351000A (en) | Culture method of pomelo hybrid | |
| CN102668975A (en) | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker | |
| CN105746337B (en) | A kind of selection of ensilage upland rice variety | |
| CN101990842A (en) | Corn hybrid high-yield seed making method | |
| CN104770288B (en) | Making method of tea tree bonsai | |
| CN1910999A (en) | Method for reproducing and producing hybrid seeds of tomato with crack-resistance and of pink color fruits | |
| CN102550397A (en) | Method for cultivating dwarf stock for grafting jatropha | |
| CN106172012A (en) | A kind of Fructus Mori tree group culturation rapid propagating technology | |
| CN101595838A (en) | A kind of method of mango artificial hybridization pollination | |
| CN106416759A (en) | Agate lycium barbarum culturing method | |
| CN108935067A (en) | The preparation method of Pennisetum inter-species triploid hydrid kind under natural conditions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140521 |