CN103756934A - Compound probiotics for cherry planting - Google Patents
Compound probiotics for cherry planting Download PDFInfo
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- CN103756934A CN103756934A CN201310754290.4A CN201310754290A CN103756934A CN 103756934 A CN103756934 A CN 103756934A CN 201310754290 A CN201310754290 A CN 201310754290A CN 103756934 A CN103756934 A CN 103756934A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to compound probiotics for cherry planting. The probiotics can be applied through soil management, microbial bacterial fertilizer, irrigation, foliage spray, ground spray, air atomization disinfection and the like. The output, quality and disease resistance of cherries are improved.
Description
Technical field
The invention belongs to microbial technology field, more specifically, relate to a kind of compound probiotic for cherry plantation.
Background technology
The phenomenons such as the cherry of ordinary method plantation is often prone to low, the easy ill insect pest of fruit-setting rate, fruit is not good, and easily shedding, dehiscent fruit, output are unstable.In cherry cultivation management process, use compound probiotic, can significantly improve generation, raising fruit quality, the output that improves of fruit-setting rate, less disease and pest.
Summary of the invention
One. the effect of compound probiotic in plantation
1. the part of plant on earth's surface is cauline leaf, and it is carrying out photosynthesis, carrying out energy transformation.
A. using the compound probiotic that contains milk-acid bacteria as foliage fertilizer, be sprayed on plant leaf and limb, milk-acid bacteria can increase the effect of light compositing bacterium, and light compositing bacterium can promote the photosynthesis of plant, the formation of fortification of plants body to vitamin C and E, therefore by take, the compound probiotic of milk-acid bacteria as master is sprayed at plant leaf and limb is upper, can promote the photosynthesis of plant, produce a large amount of chlorophyll, guaranteed luxuriance of vegetation and health.
B. as pathogenic bacteria and viral natural enemy, the safest sterilizing of milk-acid bacteria or diseases and pests of agronomic crop and sterilant.After the compound probiotic that contains milk-acid bacteria is diluted according to a certain percentage, be sprayed at plant leaf and limb upper, sprayed the cauline leaf surface journey subacidity after milk-acid bacteria, order about insect escape, also the edible food of insect that just broken, insect cannot, in the existence of cauline leaf environment, reach the effect of desinsection expelling parasite; Prodenia litura), native acarid (tick), various leaf tick worm, the great Ye grass of take be larva of food etc. thereby realize and to kill some pathogenic microorganisms and to kill some insect, as agrotis larva (formal name used at school:.Avoid a large amount of use chemical insecticides, guaranteed food safety.
2. the part of plant under earth's surface is mainly root system, and it makes plant from soil, draw various desired nutritionals, carrying out matter and energy conversion.
A. by by spoilage organism etc. cause corrupt soil, germicidal treatment before planting; A certain proportion of compound probiotic that contains milk-acid bacteria is sprayed, turned over, thereby suppress the growth of spoilage organism, make it without living space, prevent root rot.This is milk-acid bacteria " soil disinfectant " function.
B. utilize and take milk-acid bacteria and be that main compound probiotic carries out compost treatment, make microbial organic fertilizer, the soil before plantation is carried out to microbial organic fertilizer and topdress, can improve crop yield by fertilising, improve product quality, maintain and increase soil fertility.Increase soil nutrient, avoid the too much chemical fertilizer of using.
C. the compound probiotic that contains milk-acid bacteria by a certain percentage adds water and irrigates, and improves soil microorganisms environment and root exudates environment, makes its equilibrium, makes plant root more easily absorb nutrient; A minute stem phase to plant plays a part very important.
D. because milk-acid bacteria is sugary and lactic acid, and earthworm has a sharp sense of taste, happiness sweet food and tart flavour, therefore can amount reproduction as the earthworm in soil, and earthworm can be so that loosing soil, make moisture and fertilizer be easy to enter and improve the fertility of soil, be conducive to the growth of plant.
E. soil heat preservation function: due to milk-acid bacteria anaerobism breeding under soil table, driven a series of beneficial microorganisms movable, these microorganism growth metabolism are that the soil moisture improves 1-2 ℃.In the north, can play the function of protecting plant root.
F. soil water-retaining function: because milk-acid bacteria is microbial bacterial, among its cells in vivo, moisture is approximately 70%, while having a large amount of milk-acid bacteria, can keep soil moisture.Aerobe existence is at upper soll layer, and anaerobies bacterium survive under earth's surface, and spoilage organism cannot be survived under earth's surface, and assurance plant seed issues blastogenesis root, growth at the environment of safety, forms gradually ground flora, and then reaches water and soil and do not run off.
G. trophism: the milk-acid bacteria of take is that main compound probiotic is in growth metabolism process, produce multivitamin, organonitrogen, soluble phosphate, amino acid etc., when plant root nutrition can be provided, can also obviously increase the content of food protein, sugar, VITAMIN and aromatoising substance, increase the dry-matter proportion of fresh and tender food, thereby Food Quality, local flavor, keeping quality are improved, color and luster, outward appearance etc. are obviously improved, and this special role is that chemical fertilizer is irreplaceable.
H. after the compound probiotic that contains milk-acid bacteria being combined with other fibrous substances, join in soil, dig under earth's surface, carry out spontaneous fermentation, can reduce gradually nitrate ion concentration, solve acidification problem.
Under the effect of the compound probiotic that contains milk-acid bacteria, after optimumization of plant rhizosphere environment, plant stem-leaf is also just grown up healthy and sound, and insect cannot be invaded food from root; To depending on the aquaculture of plant, be also very useful, plant is as the beginning of cultivation chain, prevention to aquaculture pathogenic bacteria and virus, do not need to re-use antibiotic etc, guaranteed the pure natural property of plant, meanwhile, also reduce the residual harm bringing to people's health of environmental pollution and agricultural chemicals, thus the world that can realize nature probiotic bacterium and human life's symbiosis.
Two, the compound probiotic of different purposes forms
1. " compound probiotic " that soil remediation is used: containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.
2. make " compound probiotic " that microbial-bacterial fertilizer is used: containing compound probiotics such as milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus.
3. " compound probiotic " that pouring is used: containing compound probiotics such as milk-acid bacteria, yeast, nitrobacteria, genus bacillus.
4. cauline leaf sprays use " compound probiotic ": the compound probiotics such as photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus.
5. ground sprays use " compound probiotic ": the compound probiotics such as milk-acid bacteria, yeast, genus bacillus.
6. " compound probiotic " that air atomizing degerming is used: the compound probiotics such as milk-acid bacteria, yeast, genus bacillus.
Three. microbial species planting technology is as follows at the concrete function of strawberry cultivating:
1. increase soil fertility, chessom, maintenance soil moisture, form good rhizospheric environment, promote, outside root absorption nitrogen phosphorus potassium, to absorb iron, zinc, magnesium, manganese etc. simultaneously, impel plant leaf more healthy carry out photosynthesis.
2. increase fruit resistance, can effectively kill spoilage organism in soil, prevent and treat root rot and disease and pest; As control of the common leaf mold of strawberry, gray mold, oidium, blight etc. etc.
3. promote photosynthesis, make the more flourishing of fruit growth
4. improve fruit quality, prolong storage period
5. increasing both production and income
Embodiment
The obtain manner of compound probiotic.
Milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetes, nitrobacteria bacterial classification are all purchased from market.
Produce the fermented liquid of milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetes, nitrobacteria, then above-mentioned fermented liquid is composited in proportion first respectively.
The production method of milk-acid bacteria is:
In 1kg water, add brown sugar 0.05～1.0kg, be configured to after solution, add 5g～10g lactic acid bacteria culturers, sealed fermenting in anaerobic fermentation tank, 33～38 ℃ of controlled fermentation temperature, fermentation time 3～7 days, the liquid spawn having activated is added to second order fermentation tank sealed fermenting, second order fermentation liquid proportioning is: molasses 10%, amino acid 0.1%～0.5%, 33～38 ℃ of VITAMIN 0.05%～1% controlled fermentation temperature, fermentation time 3～7 days, inoculum size 5%～10%, between yeast phase, constantly add molasses, treat that bacterial classification concentration reaches 300,000,000/ml, pH is 4.2 o'clock, stop fermentation.
Saccharomycetic production method is:
By being inoculated into bacterial classification in yeast, expand in fermented liquid, the formula of fermented liquid is: glucose 50g/L, urea (NH
4)
2sO
41g/L, KH
2pO
42.5g/L Na
2hPO
40.5g/L, MgSO
41g/L, FeSO
40.1g/L, yeast extract paste 0.5g/L, rose-bengal 0.03g/L, pH4.5-5.0, sealed fermenting in aerobic fermentation tank, controlled fermentation temperature 25-28 ℃, fermentation time 2-3 days, the liquid spawn having activated is added to the fermentation of secondary aerobic fermentation tank, second order fermentation liquid proportioning is: soybean sprout 100g/L, glucose 50g/L, pH nature, controlled fermentation temperature 25-28 ℃, fermentation time 3～5 days, inoculum size 5%～10%, constantly adds glucose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation.
The production method of subtilis is: subtilis is seeded in substratum, culture medium prescription is: peptone 1%, yeast extract 0.5%, sodium-chlor 1%, nature pH, is placed in 37 ℃, seed fermentation tank and cultivates after 1-2 days, access fermention medium, culture medium prescription is: sucrose 1%, peptone 1%, Sodium phosphate dibasic 0.2%, SODIUM PHOSPHATE, MONOBASIC 0.2%, in natural pH, inoculum size 5%～10%, 35～37 ℃ of temperature, constantly add sucrose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation.
The production method that it is characterized in that described photosynthetic bacterium is: photosynthetic bacterium is seeded in yeast extract paste protein culture medium, and basic recipe is: CaCL
20.3g, MgSO
47H
2o0.5g, yeast extract paste 3g, peptone 3g, distilled water 1000ml, adjusts pH6.8, in culture temperature: 25 ℃～30 ℃, intensity of illumination: 2000LX～5000LX, lower cultivation 3 days, afterwards in aerobic fermentation tank with 15% inoculum size, 25 ℃～30 ℃ of temperature, intensity of illumination: 2000LX～5000LX, cultivates under the condition of pH:7.5～8.5, and in fermenting process, results of regular determination pH also carrys out neutralise broth with 150g/L Sulfothiorine, until bacterial classification concentration, reach 300,000,000/ml when above, stop fermentation.
Actinomycetic production method is: bacterial classification is inoculated in No. 1 substratum of Gao Shi (GA), and main component is: Zulkovsky starch 20g, KNO
31g, K
2hPO
40.5g, MgSO
47H
2o0.5g, NaCl0.5g, FeSO
47H
2o0.01g, pH=7.4-7.6, water 1000ml enlarged culturing is controlled 28～30 ℃ of temperature in same medium with 5%～10% inoculum size afterwards in aerobic fermentation tank, and pH7.0-7.6 fermentation culture reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
The production method of nitrobacteria is: nitrobacteria is seeded in substratum, and culture medium prescription is: ammonium sulfate 0.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, SODIUM PHOSPHATE, MONOBASIC 1g, magnesium sulfate 0.03g, CaCl
27.5g, distilled water 1000ml, in pH7.5～8.0, under 28～30 ℃ of conditions of temperature, enlarged culturing is 3 days, with 5%～10% inoculum size, in the aerobic large fermentation tank of same medium, control 28～30 ℃ of temperature afterwards, pH7.5-8.0 fermentation culture, reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
The composition of compound probiotic:
1. " compound probiotic " that soil remediation is used: milk-acid bacteria, yeast, genus bacillus, photosynthetic bacterium, actinomycetes by weight compositely proportional are: (5～8): (1～3): (0～1): (1～2): (0～1), preferably (5～6): (1～2): (0～1): (1～2): (0～1), most preferred ratio is 5:1:1:2:1.
2. make " compound probiotic " that microbial-bacterial fertilizer is used: milk-acid bacteria, yeast, genus bacillus, actinomycetes compositely proportional is by weight: (3～5): (1～2): (3～5): (1～2), preferably (3～4): (1～2): (4～5): (1～2), most preferred ratio is 3:1:4:1.
3. " compound probiotic " that pouring is used: milk-acid bacteria, yeast, genus bacillus, nitrobacteria by weight compositely proportional are: (5～8): (1～3): (1～2): 1, preferably (6～7): (1～2): (1～2): 1, most preferred ratio is: 6:2:1:1.
4. cauline leaf sprays use " compound probiotic ": milk-acid bacteria, yeast, photosynthetic bacterium, genus bacillus by weight compositely proportional are: (5～8): (1～3): (0～1): (1～2), preferably (5～6): (1～2): 1:(1～2), most preferred ratio is 5:2:1:2.
5. ground sprays use " compound probiotic ": milk-acid bacteria, yeast, genus bacillus by weight compositely proportional are: (5～8): (1～3): (1～2), what more have choosing is (6～8): (1～2): 1, and most preferred ratio is 8:1:1.
6. " compound probiotic " that air atomizing degerming is used: milk-acid bacteria, yeast, genus bacillus by weight compositely proportional are: (5～7): (2～3): (1～2), what more have choosing is (6～7): (2～3): 1, and most preferred ratio is: 6:3:1
The compound probiotic that contains milk-acid bacteria is taken as the leading factor with Lactobacterium acidophilum, and the carbohydrate that it produces by picked-up photosynthetic bacteria, yeast forms lactic acid, and milk-acid bacteria has very strong sterilizing ability, can effectively suppress the movable and organic sharply corruption of harmful microbe and decompose.It can decompose not labile xylogen and Mierocrystalline cellulose under normal conditions, and organic matter fermentation is decomposed.
According to the present planting soil corruption in planting fruit trees garden, cause the situation of various diseases, administer in accordance with the following steps:
1. for the soil that does not descend seedling
First analyze the primary condition of soil, as pH value, the critical conditions such as nematode in soil, and spoilage organism there is situation etc., determine whether will use milk-acid bacteria probiotic bacterium to carry out germicidal treatment to soil, then to draw seedling under base fertilizer.
If the root rot that exists spoilage organism (the various spoilage organism in soil) to cause in soil, needs with milk-acid bacteria sterilizing and in conjunction with irrigation method, existing soil is carried out to sterilising treatment (implementation method refers to embodiment mono-).
2. pair fruit tree soil of growing
If do not exist raw excrement etc. directly to use, cause the situation of soil corruption in soil, plant according to method described below.
After the above-mentioned the first step is to soil treatment, in plantation, use the organic farm manure of microorganism, soil is carried out to organic improving, increase content and the fertility of probiotic bacterium in soil, form good rhizospheric environment and abundant trophoplasm, increase plant root healthy, improve Root Absorption ability, improve via plant immunity, prevent root rot (implementation method refers to embodiment bis-).
3. microorganism is added irrigation, is to supplement root probiotic bacterium and trophoplasm, adds compound probiotic liquid (implementation method refers to embodiment tri-) when irrigating.
4. foliage-spray, increases the synthetic of plant chlorophyll, urges and makes blade face light compositing, improves disease-resistant, insect-prevention ability, and improves the trophoplasm (implementation method refers to embodiment tetra-) of fruit.
5. ground and trunk expelling parasite, prevents external worm, the erosion of bacterium to fruit tree from ground, and fruit tree trunk and trunk around ground spray (implementation method refers to embodiment five).
Plantation Application Example
Embodiment 1. soil sterilizings are administered
The corrupt improvement method in plantation soil
If soil does not have corruption and causes root rot, do not need to carry out this operation.Also can administer by the following method the soil before lower cherry sapling, can break off fruit tree seedlings from root like this and carry harmful bacteria.
1. soil treatment is used and to be closed probiotic bacterium stoste containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.: every 100 square meters are used after 20 times of 5kg compound probiotic stoste dilute with waters, and available atomizer is evenly sprayed at and will administers on soil.
2. compound probiotic is evenly sprayed at and will administers behind soil, with rotary cultivator, turn over one time, xylophyta is ploughed deeply 30cm(to wanting the tree of result, the degree of depth is not to hurt shallow root place, can't injure root), and then spray once-combined probiotic bacterium, with rotary cultivator, laterally turn over once, then spray hug after compound probiotic flat.Unwanted bacteria major part after for some time in soil is killed, and recovers gradually the balance of nature of soil.
This method with the following Examples 3 method use subsequently, also have good effect.
Embodiment 2. compost are produced
Embodiment 2.1 microbial organic fertilizer making methods
1. compost is used containing compound probiotic stostes such as milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus.Usage ratio is 5% to be the compound probiotic stoste that compost per ton is used 50kg to contain milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus etc., by atomizers spray, spread, compost material high humidity I is with suitable thin up probiotic bacterium stoste according to circumstances, to guarantee the normal fermentation of compost.
2. adopt artificial or mechanical mixing type to add bacterium stirring means: to turn over the loam that spreads thin layer, and then evenly sprinkle compound probiotic and stir, turn over subsequently that broadcast application is native spreads bacterium on one side.Turn over and shed after bacterium finishes, compost bottom margin, hand mixing, spills to dunghill middle and upper part being uniformly distributed in the hope of probiotic bacterium in dunghill again.Then the block muck bat in dunghill surface is broken, cover one deck plastic cloth and carry out fermentation maturity.
If conditions permit can be carried out compost by following formula: 1 cube of 1 cube of chicken manure, cow dung (also can be used fallen leaves stalk to replace or add stalk, but must be ground into 5-10mm left and right size), compound probiotic 5%, sugared slag 5%, ground phosphate rock 3%, peat composed of rotten mosses powder 3%, also can add the trace element of seldom measuring 0.5-1%, as magnesia (magnesium oxide), iron ore powder and manganese ore powder etc., the about 850kg of finished product fertilizer making, humidity is pinched the degree that can just roll into a ball with hand, approximately 50～65% left and right.Use stirrer can make to spray milk-acid bacteria and add other attached material more even, be more conducive to fertilizer fermentation, shorten fermentation time etc.
3. temperature is at more than 20 ℃ weather, and covering film anaerobic fermentation temperature rises to more than 55～60 ℃, maintains 2～3 days and can open afterwards fertile heap, carries out secondary compound probiotic and sprays compost treatment.Turn over one deck loam and spray a probiotic bacterium, concrete consumption is consumption 5-10kg per ton, then air storage.After 2～3 days, carry out again a turning, mend bacterium, after air storage for some time, can use (as conditions permit, can turning in every 3 days mend bacterium once, be conducive to becoming thoroughly decomposed of fertilizer).
4. at the beginning of compost is preferably in annual 4 months, start, weather temperature raises, and processes and can do base fertilizer use in 1-2 month.
5., if the finished product fertilizer of use buying is as base fertilizer, the compound probiotic that can be directly adds 2kg to contain milk-acid bacteria etc. according to every 100kg fertilizer, adds water 5kg to stir, and applies to plant in soil and makees base fertilizer.
6. in order to guarantee the effect of compost, not mixed with the series products of other starters while using this product.
Embodiment 2.2, cherry tree fallen leaves carry out compost utilization
Probiotic bacterium+stalk (fallen leaves) compost
A. use pulverizer that stalk (fallen leaves) is ground into 5-10mm large;
B. dig in the ground one dark 1 meter, the heatable adobe sleeping platform that the long X of certain size is wide, length and width are determined according to the amount of stalk (fallen leaves), not too big (about 5-15 ㎡) in principle, no person is not high to utilizing in soil bacterium effect; The immature soil digging out, gives over to stand-by;
C. stalk (fallen leaves) is put into the end, hole, during 5 centimeters of thickness, sprayed living lactic acid bacteria 4-5L/ ㎡, then spray raw excrement 5kg/ ㎡ (people's animal dung all can), during without raw excrement, also can use, repave the immature soil (do not have large bulk in the immature soil, be loose shape as far as possible) of 3 centimeters of left and right; And then put into successively stalk (fallen leaves) → probiotic bacterium → life excrement → immature soil, until during 10 centimeters, tool earth's surface, compacting as far as possible, by Bas Discharged;
For observing leavening temperature, can be at centre-height place an embedding electronic thermometer, display screen extends on limit, hole, ground, to observe;
D. use plastics film to cover surface, surrounding compacting, places air and enters from surrounding;
E. on burying on film, the immature soil is to floor level again;
F. in fermenting process, observe temperature, if core temperature reaches 40-55 degree or higher, and can continue this temperature 1-2 days or more than, prove that fermenting process make good progress.Fermentation 30-45 days, according to different time length difference in season;
G. the fertilizer fermenting is taken out and used, or pack is preserved; Preferably according to 1% of amount of organic fertilizer, spray probiotic bacterium before use again, after stirring, re-use, effect can be better.
Embodiment 2.3 probiotic bacteriums+chicken manure organic compost
Do not use the chicken manure of probiotic bacterium cultivation to carry out organic compost:
A. measure the water content of chicken manure and the ammonia amount of release, the live chickens excrement of caking is pulverized;
B. according to the water content of chicken manure and stink, according to the ratio of 5-10%, add probiotic bacterium, use stirrer fully to stir;
C. pack sealing, puts into plastic containers, and compacting is also extruded air, then container sealing is good as far as possible;
D. place 30-45 days with top fermentation, then take out and dry dehydration, pack to be used.
Application process: according to the requirement of different fruit trees, execute at root or soil table and all can; With root, extend under certain distance and bury better, what lower buried depth degree depended on fruit tree prolongs the root degree of depth.
Generally, annual minute 4 fertilisings are advisable, and fertilization time is respectively:
1. fertile before rudiment
2. fertile after fallen flowers
3. Fruit is fertile
4. the fertilising of base manure fruit tree should be take base manure as main.Time of adding is best with autumn.
Fertilization effect: the 1. 2. time fertilising can effectively promote rudiment, bloom and prevent in time to consume because blooming a large amount of nutrients and produce de-fertilely, improve fruit-setting rate, promotion New shoot growth.The 3. time to topdress be in order to meet the needs of Fruit, shoot and leaf growth and bud differentiation.4. the effect of bulk application be after guaranteeing to gather to Photosynthesis of Fruit before fallen leaves, improve nutrient accumulation, for next year growth and development of fruit tree lay a solid foundation.
Fertilizing method: tree body is hour general adopts ring-type fertilising, being advisable with 0.5～1.5 meter of the periphery of tree crown in the position of fertilising, opens ditches wide 20～40 centimetres, dark 20～30 centimetres, applies in ditch after mixing fertilizer and soil are appropriate, then ditch is filled and led up.Adult fruit tree preferably adopts the fertilising of full garden, in conjunction with intertillage, fertilizer is ploughed under in soil.Because the root growth of fruit tree has significantly to fertilizer, preferably apply 30～40 centimetres of dark deep subsoils, to improve the depth & wideth of Root Distribution, strengthen the drought-resistant ability of fruit tree and tree body property admittedly.
Embodiment 3. fills with radical cure reason
Filling with general uses containing dilution compound probiotics such as milk-acid bacteria, yeast, nitrobacteria, genus bacillus.500～1000 times of compound probiotic stoste dilute with waters, carry out according to the normal irrigation water yield.According to the acidproof situation of fruit variety, can be every inferior or repeatedly interpolation.
Embodiment 4. foliage-sprays
Compound probiotic foliage-spray method
1. foliage-spray: just can carry out the compound probiotic (containing the compound probiotic of photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus etc.) that cauline leaf sprays dilution after fruit tree leafing, probiotic bacterium can promote the effect of light compositing bacterium, and light compositing bacterium can directly promote the photosynthesis of plant.The compound probiotic of dilution is in proportion sprayed on blade, produces a large amount of chlorophyll, guarantee luxuriance of vegetation and health.It is the absorption that plant stem-leaf has additional nutrients that the metabolic activity of compound probiotic can provide amino acid and various VITAMIN, Promoting plant growth, and can play the effect (full-bloom stage can not spray) of sterilization, and after result, still can spray, according to circumstances can within every 10 days, spray once.
2. cauline leaf sprays and uses dilution compound probiotic: by 500～1000 times of compound probiotic dilute with waters, every mu of ground is used about 200L compound probiotic diluent, is on the vaporific cauline leaf that is evenly sprayed at plant and on limb.
If atomizer sprayed in the past chemical sterilization sterilant and so on, re-use after do cleaning up.
Embodiment 5. ground and trunk deinsectization expelling parasite are processed
Compound probiotic ground spraying method
1. use the compound probiotic stoste that contains milk-acid bacteria, yeast, genus bacillus etc., around main trunk, in the diameter range of 1～2 meter, with every consumption of setting 50～100 milliliters, spray, can strengthen usage quantity according to disease and insect amount.
2. main trunk sprays, in the 1 meter of altitude range that makes progress from ground along trunk.
3. start to spray once weekly, reduce gradually, until situation alleviate or disappear after stop.
Precaution: compound probiotic is living microorganism, can't mixes use with other chemical fertilizer, agricultural chemicals.
Experiment effect comparison
By buying conventional compound probiotic microbial inoculum in contrast on market, commercially available prod 1 be the probiotic bacterium that Beijing company sells, and contains that to take photosynthetic bacteria, milk-acid bacteria, yeast be probiotic bacterium and the meta-bolites of leading; Milk-acid bacteria be take as main compound probiotic for what sold bio tech ltd, Yichuan in commercially available prod 2.
Experimental planting cherry variety is large red light.
Compound probiotic (experimental group 1), the commercially available prod 1(experimental group 2 of in equal experiment condition Xia Yi our company, producing), commercially available prod 2(experimental group 3) and do not use compound probiotic (control group) to plant processing to cherry, in the on all four situation of Cultivate administration mode, cherry fruit is detected, and result is as follows:
1, experimental group 1 cherry output improves 22.3% compared with control group, compared with experimental group 2, improves 15.2%, compared with experimental group 3, improves 12.8%.
2, cherry fruit experimental group is vivider full compared with control group outward appearance, and size is homogeneous more.
3, disease and pest experimental group obviously reduces compared with control group, and dehiscent fruit rate experimental group 1 reduces by 31% compared with control group, and experimental group 1 reduces by 24% compared with experimental group 2, compared with experimental group 3, reduces by 13%.
4, cherry mouthfeel is stronger compared with control group, sour-sweet moderate, juice is many, quality has clear improvement, protein,, Vc, iron equal size be all significantly increased, detailed data sees the following form, table 1 is experimental group 1 and control group Quality Detection contrast table, table 2 be experimental group 1 with to experimental group 2 quality inspections survey contrast tables, table 3 be experimental group 1 and experimental group 3 Quality Detection contrast tables.
Table 1 experimental group 1 and the contrast of control group Quality Detection
Table 2 experimental group 1 with experimental group Quality Detection is contrasted
Table 3 experimental group 1 and experimental group 3 Quality Detection contrasts
Claims (10)
1. for a compound probiotic for cherry plantation, mainly by milk-acid bacteria, formed.
2. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that soil remediation is used, containing milk-acid bacteria, yeast, actinomycetes bacterium, photosynthetic bacterium, genus bacillus etc.
3. probiotic bacterium claimed in claim 1, it is characterized in that can be used for making the compound probiotic that microbial-bacterial fertilizer is used: containing milk-acid bacteria, yeast, actinomycetes bacterium, genus bacillus etc.
4. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that pouring is used: containing milk-acid bacteria, yeast, nitrobacteria, genus bacillus etc.
5. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that cauline leaf sprays use: containing photosynthetic bacterium, milk-acid bacteria, yeast, genus bacillus etc.
6. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that ground sprays use: containing milk-acid bacteria, yeast, genus bacillus etc.
7. probiotic bacterium claimed in claim 1, it is characterized in that can be used for the compound probiotic that air atomizing degerming is used: containing milk-acid bacteria, yeast, genus bacillus etc.
8. the application of probiotic bacterium in cherry plantation described in claim 1-7, is characterized in that being produced, being filled with by soil sterilizing improvement, compost the modes such as radical cure reason, foliage-spray, ground deinsectization expelling parasite processing and air atomizing sterilizing insect-killing and applies.
9. the preparation method of probiotic bacterium described in claim 1-7, is characterized in that: produce first respectively milk-acid bacteria, yeast, subtilis, photosynthetic bacterium, actinomycetic fermented liquid, then above-mentioned fermented liquid is composited by weight.
10. the compound probiotic preparation method described in claims 9, is characterized in that:
The production method of described milk-acid bacteria is: in 1kg water, add brown sugar 0.05～1.0kg, be configured to after solution, inoculum size with 1%～5% adds lactic acid bacteria culturers, sealed fermenting in anaerobic fermentation tank, 33～38 ℃ of controlled fermentation temperature, fermentation time 3～7 days, the liquid spawn having activated is added to second order fermentation tank sealed fermenting, second order fermentation liquid proportioning is: molasses 10%, amino acid 0.1%～0.5%, 33～38 ℃ of VITAMIN 0.05%～1% controlled fermentation temperature, fermentation time 3～7 days, inoculum size 5%～10%, between yeast phase, constantly add molasses, treat that bacterial classification concentration reaches 300,000,000/ml, pH is 4.2 o'clock, stop fermentation,
Described saccharomycetic production method is:
By being inoculated into bacterial classification in yeast, expand in fermented liquid, the formula of fermented liquid is: 50g/L, urea (NH
4)
2sO
41g/L, KH
2pO
42.5g/L Na
2hPO
40.5g/L, MgSO
41g/L, FeSO
40.1g/L, yeast extract paste 0.5g/L, rose-bengal 0.03g/L, pH4.5-5.0, sealed fermenting in aerobic fermentation tank, controlled fermentation temperature 25-28 ℃, fermentation time 2-3 days, the liquid spawn having activated is added to the fermentation of secondary aerobic fermentation tank, second order fermentation liquid proportioning is: soybean sprout 100g/L, glucose 50g/L, pH nature, controlled fermentation temperature 25-28 ℃, fermentation time 3～5 days, inoculum size 5%～10%, constantly adds glucose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation;
The production method of described subtilis is: subtilis is seeded in substratum, culture medium prescription is: peptone 1%, yeast extract 0.5%, sodium-chlor 1%, nature pH, is placed in 37 ℃, seed fermentation tank and cultivates after 1-2 days, access fermention medium, culture medium prescription is: sucrose 1%, peptone 1%, Sodium phosphate dibasic 0.2%, SODIUM PHOSPHATE, MONOBASIC 0.2%, in natural pH, inoculum size 5%～10%, 35～37 ℃ of temperature, constantly add sucrose between yeast phase, when bacterial classification concentration reaches 300,000,000/ml, stop fermentation;
The production method of described photosynthetic bacterium is: photosynthetic bacterium is seeded in yeast extract paste protein culture medium, and basic recipe is: CaCL
20.3g, MgSO
47H
2o0.5g, yeast extract paste 3g, peptone 3g, distilled water 1000ml, adjusts pH6.8, in culture temperature: 25 ℃～30 ℃, intensity of illumination: 2000LX～5000LX, lower cultivation 3 days, afterwards in aerobic fermentation tank with 15% inoculum size, 25 ℃～30 ℃ of temperature, intensity of illumination: 2000LX～5000LX, cultivates under the condition of pH:7.5～8.5, and in fermenting process, results of regular determination pH also carrys out neutralise broth with 150g/L Sulfothiorine, until bacterial classification concentration, reach 300,000,000/ml when above, stop fermentation; Described actinomycetic production method is: bacterial classification is inoculated in No. 1 substratum of Gao Shi (GA), and main component is: Zulkovsky starch 20g, KNO
31g, K
2hPO
40.5g, MgSO
47H
2o0.5g, NaCl0.5g, FeSO
47H
2o0.01g, pH=7.4-7.6, water 1000ml enlarged culturing is controlled 28～30 ℃ of temperature in same medium with 5%～10% inoculum size afterwards in aerobic fermentation tank, and pH7.0-7.6 fermentation culture reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation;
The production method of described nitrobacteria is: nitrobacteria is seeded in substratum, and culture medium prescription is: ammonium sulfate 0.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, SODIUM PHOSPHATE, MONOBASIC 1g, magnesium sulfate 0.03g, CaCl
27.5g, distilled water 1000ml, in pH7.5～8.0, under 28～30 ℃ of conditions of temperature, enlarged culturing is 3 days, with 5%～10% inoculum size, in the aerobic large fermentation tank of same medium, control 28～30 ℃ of temperature afterwards, pH7.5-8.0 fermentation culture, reaches 300,000,000/ml when above until bacterial classification concentration, stops fermentation.
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| CN104045428A (en) * | 2014-05-29 | 2014-09-17 | 丹阳市保得生态农林开发有限公司 | Cherry potting nutrient soil |
| CN105483042A (en) * | 2015-12-24 | 2016-04-13 | 贵州红瑞祥农业发展有限公司 | Method improving disease resistance of trunk |
| CN105541512A (en) * | 2015-12-04 | 2016-05-04 | 北京益生动力生物科技有限公司 | Ecological bacterial fertilizer and natural farming ecological planting method |
| CN106471935A (en) * | 2016-10-13 | 2017-03-08 | 合肥润雨农业科技有限公司 | A kind of processing method of european large cherry seed |
| CN107827488A (en) * | 2017-12-12 | 2018-03-23 | 杨志军 | A kind of liquid-state composite bacterial microbe and preparation method thereof |
| CN109197305A (en) * | 2018-07-19 | 2019-01-15 | 贵州省望谟县明灿芒果种植有限公司 | A kind of mango implantation methods |
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| CN109197305A (en) * | 2018-07-19 | 2019-01-15 | 贵州省望谟县明灿芒果种植有限公司 | A kind of mango implantation methods |
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