CN103751817A - Method for producing antibacterial property of object surface by lactobacillus fermentation product - Google Patents

Method for producing antibacterial property of object surface by lactobacillus fermentation product Download PDF

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Publication number
CN103751817A
CN103751817A CN201110319772.8A CN201110319772A CN103751817A CN 103751817 A CN103751817 A CN 103751817A CN 201110319772 A CN201110319772 A CN 201110319772A CN 103751817 A CN103751817 A CN 103751817A
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mentioned
object surface
antibiotic activity
lactobacillus
lactobacillus fermentation
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CN201110319772.8A
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朱琴
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Individual
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Abstract

The invention relates to a method for producing the antibacterial property of the object surface by a lactobacillus fermentation product. The method is characterized in that the object surface is coated with a mixed solution of a lactobacillus fermentation product and a binder. The method utilizes the lactobacillus fermentation product having a wide antimicrobial spectrum so that antibacterial properties of household electrical appliance products can be fully performed.

Description

Utilize the method for lactobacillus fermentation to impart antibiotic activity on object surface
Technical field:
The present invention relates to utilize the method for lactobacillus fermentation to impart antibiotic activity on object surface, say so that more specifically the lactobacillus fermented product of antibacterial and antiviral effect and the mixed solution coating of the binding agent lip-deep method at the various objects that may contact, breed such as antibacterial or viruses will be there is.
Background technology
Recently, along with the concern to surrounding increases year by year, user need to give antimicrbial power in the life household appliances directly related with human physiology, thereby can meet user's health demand and close environmental demand simultaneously.Wherein, need to keep health, clean household appliances to have the refrigerator of comprising, washing machine, air-conditioner, vacuum cleaner, dehydrator, electric food warmer, electric cooker, dish-washing machine, microwave oven, VTR, computer, TV etc. multiple.
For endowing antibacterial energy in above-mentioned household appliances, generally use organic and inorganic antibacterial etc.But, using organic antibacterial agent in the situation that, will be difficult to lasting its result of use, and to the stability action of human body also a little less than.Lactobacillus fermented product is to draw product in the sweat by the Pickles as Korean traditional diet, above-mentioned lactobacillus fermented product is by absorbing in human body for a long time, thereby can guarantee its stability, and there is easy picked-up and comparatively economic, be that a kind of antimicrbial power is higher, and there is the natural antibacterial material of wider antimicrobial spectrum.
And, result draws in the light of recent researches, prevention and the treatment of the viral disease of lactobacillus fermented product to poultries such as bird flus have good effect, and the antibacterial and antiviral effect of the present inventor based on above-mentioned lactobacillus fermented product proposed the present invention.
Summary of the invention
The object of the present invention is to provide and a kind ofly will there is the lactobacillus fermented product of antibacterial and antiviral effect and the mixed solution coating of the binding agent lip-deep method at the various objects that may contact, breed such as antibacterial or viruses.
Realize above-mentioned purpose, the present invention is characterized in that: by the mixed solution coating of lactobacillus fermented product and binding agent on the surface of object.
The group that above-mentioned lactobacillus fermented product can form from dry thing of the concentrated solution of the culture fluid by pickle lactic acid bacteria, culture fluid, culture fluid and composition thereof, select.Above-mentioned pickle lactic acid bacteria can be selected from the group who consists of Leuconostoc (Leuconostoc) pickle lactic acid bacteria, Lactobacillus (Lactobacillus) pickle lactic acid bacteria, Wei Si Bordetella (Weissella) pickle lactic acid bacteria and composition thereof.The group that above-mentioned binding agent can form from the acrylic resin by silicones modification, polyurethane resin, acrylic resin and silicones, select.
Above-mentioned object can be the various objects, particularly life household appliances that antibacterial or virus etc. may contact, breed, for example refrigerator, washing machine, air-conditioner, air purifier, vacuum cleaner, dish-washing machine, microwave oven, or the internal structure external structure parts of the said goods.And the present invention is characterized in that: above-mentioned object is filter.
And, in above-mentioned coating step, the mixed solution of lactobacillus fermented product and binding agent is sprayed at body surface, or object is immersed in above-mentioned mixed solution.
The method of utilizing lactobacillus fermentation to impart antibiotic activity on object surface according to the present invention, has utilized the lactobacillus fermented product with wider antimicrobial spectrum in the present invention, thereby can give full play to the anti-microbial property of household appliances object.And the antiviral property having by pickle lactic acid bacteria, can be to causing that the virus of bird flu reaches stronger safely and effectively antiviral effect.
The specific embodiment
Below, the present invention will be described in detail.
Lactobacillus fermented product in the present invention obtains in the maturation process of Pickles.The pickle lactic acid bacteria using in the present invention can be by directly extracting use from Pickles, and maybe can buy the pickle lactic acid bacteria of selling on the market and be cultivated use, and, can use the lactobacillus of any state, and not there is special restriction.
And the culture fluid of above-mentioned pickle lactic acid bacteria can be that to select the group who forms from the concentrated solution of the culture fluid by pickle lactic acid bacteria, culture fluid, dry thing of culture fluid and composition thereof is a certain.
As the binding agent in the present invention, can use silicones acrylic resin modified (siliconemodified acrylicc resin), polyurethane resin, acrylic resin and silicones etc., but also not be defined in this, can use multiple binding agent.In the situation that using binding agent, lactobacillus fermented product can be easy to be solidificated on body surface, and improve the active force that mutually combines of body surface and lactobacillus fermented product, thereby can reduce the flood rate of lactobacillus fermented product, and keep good anti-microbial property.
Object in the present invention can be the various objects, particularly life household appliances that antibacterial or virus etc. may contact, breed, for example, and refrigerator, washing machine, vacuum cleaner, air purifier, air-conditioner, dish-washing machine, various device for cooking etc.And above-mentioned object can be each structure member of the inside and outside of the various household appliances of formation.Wherein, object raw material is not particularly limited, as long as forming the inside and outside of household appliances, can use any material, for example, can use the materials such as thermoplastic resin or thermosetting resin, rubber, metal, above-mentioned material can be used according to different purposes according to the characteristic of its material.
And the object in the present invention can be air cleaning filter, now, as object raw material, need only.The function with air cleaning filter, will be without special restriction in its kind, shape, size and manufacture method.For example, can use the glass fibre of glass fibre, ion-exchange fibre, cellulose fibre, asbestos fibre etc. and various organic fiber, inorfil, or can use metal or the plastics of zinc, copper, aluminum etc., above-mentioned material can be divided into different purposes according to its material characteristic to be used.And it can use honeycomb type, corpuscular type, net type, filter paper type, cotton type, aperture plate type, sheet type and template etc. in shape, there is no particular restriction, can suitably be out of shape and use according to the air cleaner of application.Above-mentioned filter can be applicable to deodorize filter, the high-performance filters (HEPA filter) such as the active carbon filter using in the multiple household electrical appliances products such as refrigerator or air-conditioner, and use as filters such as the air cleaners of automobile, or use in the lump with above-mentioned each filter.And the object in the present invention is except air cleaning filter, also applicable in water-purifying filter.
In the process on object by lactobacillus fermented product coating, lactobacillus fermented product is mixed with binding agent, then above-mentioned mixed solution is injected in to the surface of object by spray regime, or object is immersed in the container that fills above-mentioned mixed solution.As long as lactobacillus fermented product can mix with binding agent in the degree of body surface with coating, will be without special restriction in the manufacture method of above-mentioned mixed solution.The concentration of above-mentioned lactobacillus fermented product is 5-20 % by weight preferably, but is not defined in this,
Can as required and suitably regulate and use.And, in the content ratio of lactobacillus fermented product and binding agent, there is no special restriction, can as required and suitably regulate and use.
In addition, as the step before coating, can add following steps: by the clean target object of suitable washing water, and dry above-mentioned clean target object by heat treatment operation etc.And, as the step after coating, can add following steps: by heat treatment operation etc., dry.In the situation that drying object, its drying time and baking temperature, can regulate according to the shape of the object using, kind, size etc.
And according to circumstances, for the object of metal etc., be preferably in and in its manufacture process or keeping, remove the upper oil formulation of pasting in its surface.
Below, will the embodiment in the present invention be described in detail, but the present invention is not defined in embodiment as described below.
Embodiment 1
The alum gate net of buying from air filter company was immersed in 2.5%NaOH about 3 minutes, and removes oil formulation, then, the alum gate net of above-mentioned removal oil formulation is cleaned with 2.5%NaOH, aforesaid operations carries out 7 times repeatedly.Then, by above-mentioned, obtain clean alum gate net and be placed in the oven dry baking box that is adjusted to 40 ℃ of temperature, carry out 2 hours heat treatments.Simultaneously, by lactobacillus fermented product and silicones modified propylene acid binder, the ratio take lactobacillus fermented product as 5-20 % by weight is mixed, and make mixed solution, then above-mentioned mixed solution is sprayed and carries out coating on the net at above-mentioned heat treated alum gate, consequently, by obtaining coating, there is the alum gate net of pickle lactic acid bacteria.
Embodiment 2
In order to detect air cleaning filter that the coating of making as mentioned above has the lactobacillus fermented product anti-microbial property to coliform, staphylococcus aureus and bacillus pyocyaneus, according to the method for KS M 0146-2003, its sterilizing power is tested.
; utilize the assay devices such as high pressure sterilization device, antibacterial culturing thermostat, sterile board, colonometer (ColonyCounter), thermostatic water bath, extinction photometer, the bacterial strain of coliform (EscherichiacoATCC 25922), staphylococcus aureus (StaphylOCOCCtls aureus ATCC 6538) and bacillus pyocyaneus (Pseudomonas aeruginosa ATCC 27853) has been carried out respectively to sterilizing power test.Above-mentioned bacterial strains is coated in (each 1.0ml) on the test test portion of the alum gate web filter 1.0x1.0cra size in the present invention who obtains by Production Example, simultaneously, by 3 contrast test portions of an inoculation test bacterium liquid, 3 test test portions are positioned in conical flask (Erlenmeyer flask), and cultivate 1 hour, 2 hours, 3 hours under 35 ± 1 ℃, the condition of 120RPM.Then, extract above-mentioned bacterium liquid, and utilize normal saline to dilute, and under the temperature conditions of 37 ℃, cultivate 48 hours in dull and stereotyped incubator, confirm bacterium quantity, its result is with reference to if following table 1 is to as shown in table 3.
Table 1: to the sterilizing power of coliform
Figure BSA00000595087500041
Table 2: to the sterilizing power of staphylococcus aureus
Figure BSA00000595087500042
Table 3: to the sterilizing power of bacillus pyocyaneus
Figure BSA00000595087500051
Known to table 3 according to table 1 as above, the air cleaning filter that comprises pickle lactic acid bacteria in the present invention is by the bactericidal effect significantly improving microorganisms such as coliform, staphylococcus aureus, bacillus pyocyaneus.
Application Example
Method in the present invention is applicable to various uses as described below, by the object of the method manufacture in the present invention, being the multiple household electrical appliances product that need to possess anti-microbial property, can be refrigerator, washing machine, air-conditioner, vacuum cleaner, air purifier, water purifier, humidifier, dish-washing machine, microwave oven, VTR, computer, TV etc.
1) application in refrigerator can be applicable to inwall, cool-air duct, blowing fan, refrigerator doors bundle, vegetables box, air cleaning filter, ice maker, the bracket of refrigerator, surface of refrigerator outside etc.
2) application in washing machine
Can coating on the surface of the washing machine structure parts such as inside groove, water jacket, vibrosieve, detergent cylinder, cylinder lifting device, washing tub, and, also can be applicable to the filter (air cleaning filter, water-purifying filter) in dry stream, plumbing stream and circulation stream.
3) application in air-conditioner can be applicable to the cooling pin (pin) in heat exchanger, electric heating switch (ventilation element), blowing fan (metal, injected material), air flow circuit (sucting, discharge portion, shutter, grid etc.), and the air cleaning filter of indoor set.
4) effect in air purifier
Lactobacillus fermented product filter can be installed in addition in the multi-filter of air purifier.
5) application in vacuum cleaner
The in the situation that of vacuum cleaner, owing to directly catching, tactile situation is more, therefore can be to the surface coating of vacuum cleaner.And, also can carry out coating processing to the inside of dust cylinder, thereby obtain further bactericidal effect in dust cylinder, in addition, also can be applicable in the discharge filter of vacuum cleaner.
6) application in dish-washing machine can be applicable to wash the dishes cylinder, water leg, adopt in dirty net, spray arm, water-purifying filter etc.

Claims (9)

1. a method of utilizing lactobacillus fermentation to impart antibiotic activity on object surface, is characterized in that: by the mixed solution coating of lactobacillus fermented product and binding agent on the surface of object.
2. the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, is characterized in that: above-mentioned object is the household appliances such as refrigerator, washing machine, air-conditioner, air purifier, dish-washing machine.
3. the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface according to claim 2, is characterized in that: above-mentioned object is the internal structure external structure parts of household appliances.
4. the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, is characterized in that: above-mentioned object is filter.
5. the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, is characterized in that: the group that above-mentioned binding agent forms from the acrylic resin by silicones modification, polyurethane resin, acrylic resin and silicones, select.
6. the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, is characterized in that: the group that above-mentioned lactobacillus fermented product forms from the concentrated solution of the culture fluid by pickle lactic acid bacteria, culture fluid, dry thing of culture fluid and composition thereof, select.
7. according to the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface described in claim 1 or 6, it is characterized in that:
Above-mentioned pickle lactic acid bacteria is selected from the group who consists of Leuconostoc pickle lactic acid bacteria, Lactobacillus pickle lactic acid bacteria, Wei Si Bordetella pickle lactic acid bacteria and composition thereof.
8. the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, it is characterized in that: in above-mentioned coating step, the mixed solution of lactobacillus fermented product and binding agent is sprayed at body surface, or object is immersed in above-mentioned mixed solution.
9. according to the method for utilizing lactobacillus fermentation to impart antibiotic activity on object surface described in claim 1 or 8, it is characterized in that: lactobacillus fermented product contains the amount of 5-20 % by weight in mixed solution.
CN201110319772.8A 2011-10-20 2011-10-20 Method for producing antibacterial property of object surface by lactobacillus fermentation product Pending CN103751817A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108310441A (en) * 2017-01-18 2018-07-24 南京理工大学 A method of improving mobile phone screen surface flora

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108310441A (en) * 2017-01-18 2018-07-24 南京理工大学 A method of improving mobile phone screen surface flora

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