CN101085368A - Method for endowing antibacterial performance to object surface by pickle lactobacillus leavening - Google Patents
Method for endowing antibacterial performance to object surface by pickle lactobacillus leavening Download PDFInfo
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- CN101085368A CN101085368A CN 200610014186 CN200610014186A CN101085368A CN 101085368 A CN101085368 A CN 101085368A CN 200610014186 CN200610014186 CN 200610014186 CN 200610014186 A CN200610014186 A CN 200610014186A CN 101085368 A CN101085368 A CN 101085368A
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Abstract
A method for enduing object surface with micro-organism resistance by using of kraut lactobacillus leavening comprises coating kraut lactobacillus leavening, agglomerant and nanometer metallics on object surface simultaneously or in sequence. Object is household appliance such as refrigerator, washing machine, air conditioner, air purifier, and dishwasher; inside and outside structure member of household appliance and filter. Nanometer metallics are metallics having sterilizing function. Metallics are particle of silver, zinc, copper, platinum, iron, cadmium, palladium, rhodium, and chromium which can be exclusively used or used as rafting. The invention coats antibiotic and antiviral kraut lactobacillus leavening and nanometer metallics on different kinds of object with possible contact or progenition of bacteria or virus especially on the surface of household appliance to make them have micro-organism resistance.
Description
Technical field
The present invention relates to a kind of method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface.
Background technology
Recently, along with the concern to surrounding increases year by year, the user need give antimicrbial power to the life household appliances directly related with the human body health, thereby can satisfy user's health demand and close environmental demand simultaneously.Wherein, need to keep the household appliances of health, cleaning to have the refrigerator of comprising, washing machine, air-conditioner, vacuum cleaner, dehydrator, electric food warmer, electric cooker, dish-washing machine, microwave oven, VTR, computer, TV etc. multiple.
For to above-mentioned household appliances endowing antibacterial energy, generally use organic and inorganic antibacterial etc.But, using under the situation of organic antibacterial agent, will be difficult to continue its result of use, and to the stability action of human body also a little less than.
Pickle lactobacillus fermentation is the product that draws by in the sweat as the Pickles of Korean traditional diet, above-mentioned pickle lactobacillus fermentation is by absorbing for a long time in the human body, thereby can guarantee its stability, and have easy picked-up and a comparatively economic advantage, be that a kind of antimicrbial power is higher, and have the natural antibacterial material of wider antimicrobial spectrum.
And nano metal particles shifts metal by silver (Ag), zinc (Zn), copper (Cu) etc., forms with the microminiaturization of nanometer size, and the antibacterial effect of above-mentioned transfer metal is generally well-known.
And, the result draws in the light of recent researches, pickle lactobacillus fermentation has good effect to the prevention and the treatment of the viral disease of poultries such as bird flu, and the present inventor has proposed the present invention based on the antibiotic and antiviral effect of above-mentioned pickle lactobacillus fermentation.
Summary of the invention
Technical problem underlying to be solved by this invention is, overcome the defective that existing technology exists, and provide a kind of method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, to have simultaneously or the successively coating various objects that may contact, breed of the pickle lactobacillus fermentation of antibiotic and antiviral effect and nano metal particles in antibacterial or virus etc., particularly coating makes it have antibiotic property in the surface of life household appliances.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface is characterized in that: with pickle lactobacillus fermentation, binding agent and nano metal particles simultaneously or successively coating on object surfaces.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein object is household appliances such as refrigerator, washing machine, air-conditioner, air purifier, dish-washing machine.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein object is the internal structure external structure parts of household appliances.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein object is a filter.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein nano metal particles is the metallic with sterilizing function.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein metallic is the particle of silver, zinc, copper, platinum, ferrum, cadmium, palladium, rhodium, chromium, it can use separately or use as the alloy more than 2 kinds.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein binding agent is from by the mixture of selecting a resin or several resins modification silicon acrylic resin, polyurethane resin, acrylic resin and the silicones arbitrarily.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein pickle lactobacillus fermentation is selected from the culture fluid of pickle lactic acid bacteria, the concentrated solution of culture fluid, dry thing of culture fluid and composition thereof.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein pickle lactic acid bacteria is from by selecting Leuconostoc pickle lactic acid bacteria, Lactobacillus pickle lactic acid bacteria, Wei Si Bordetella pickle lactic acid bacteria and composition thereof.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein in above-mentioned coating step, the mixed solution of pickle lactobacillus fermentation, nano metal particles and binding agent is sprayed at body surface, or object is immersed in the above-mentioned mixed solution.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein the percentage by weight of pickle lactobacillus fermentation in above-mentioned mixed solution is 5～20%.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein the content of nano metal particles is below the 100ppm to 2000ppm in the mixed liquor.
The aforesaid method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, wherein in above-mentioned coating step, with above-mentioned nano metal particles at first coating on body surface, subsequently the pickle lactobacillus fermentation coating is had in above-mentioned coating on the object of nano metal particles, carry out coating operation successively.
The specific embodiment
Pickle lactobacillus fermentation among the present invention obtains in the maturation process of Pickles.The pickle lactic acid bacteria that uses among the present invention can maybe can be bought the pickle lactic acid bacteria of selling on the market it is cultivated use, and can use the lactobacillus of any state, and be not to have special qualification by directly extracting use from Pickles.And the culture fluid of above-mentioned pickle lactic acid bacteria can be select the culture fluid from pickle lactic acid bacteria, the concentrated solution of culture fluid, dry thing of culture fluid and composition thereof a certain.
Nano metal particles among the present invention is so long as have sterilizing function and get final product, and without particular limitation, can use multiple metallic.For example, above-mentioned metallic can use separately for silver (Ag), zinc (Zn), copper (Cu), platinum (Pt), ferrum (Fe), cadmium (Cd), palladium (Pd), rhodium (Rh), chromium metallics such as (Cr), or uses as the alloy of metal more than 2 kinds.Above-mentioned nano metal particles refers to the metallic that carries out nanorize, and it is not particularly limited on manufacture method, so long as with the microminiaturized particle of nanometer size, can use any metallic.Above-mentioned nano metal particles can suppress the breeding function of microorganisms such as bacillus, mycete, thereby the breeding of restriction micro-organisms is penetrated in the cell simultaneously, and the effect of the enzyme (enzyme) that needs when stopping microbial respiratory, thereby restrain its metabolism, and sterilize.Particularly, consider antibacterial action, to the stability of environment and human body etc., preferably use silver (Ag), zinc (Zn) and copper (Cu) metallic, wherein preferably use silver (Ag) metallic.
Can use modification silicon acrylic resin (silicone modifiedacrylic resin), polyurethane resin (urethane resin), acrylic resin (acrylic acidresin) and silicones (silicone resin) etc. as the binding agent among the present invention, but be not to be defined in this yet, can use multiple binding agent.Under the situation of using binding agent, can be easy to be solidificated in pickle lactobacillus fermentation on the body surface, and improve the active force that mutually combines of body surface and pickle lactobacillus fermentation, thus the flood rate of pickle lactobacillus fermentation can be reduced, and keep good antibacterial performance.
Various objects, particularly life household appliances that object among the present invention can may contact, breed for antibacterial or virus etc., for example, refrigerator, washing machine, vacuum cleaner, air purifier, air-conditioner, dish-washing machine, various device for cooking etc.And above-mentioned object can be each structure member of the inside and outside that constitutes various household appliances.Wherein, the object raw material is not particularly limited, so long as can constitute the inside and outside of household appliances, can use any material, for example, can use materials such as thermoplastic resin or thermosetting resin, rubber, metal, above-mentioned material can be used for different purposes according to the characteristic of its material.
And the object among the present invention can be air cleaning filter, so long as have the function of air cleaning filter, will not have special qualification as the object raw material this moment on its kind, shape, size and the manufacture method.For example, can use glass fibre, ion-exchange fibre, cellulose fibre (cellulosefiber), asbestos fibre inorfil and various organic fibers such as (asbestos fiber), perhaps can use the metal or the plastics of zinc, copper, aluminum etc., above-mentioned material can be used for different purposes according to its material characteristic.And, it can use honeycomb type (honeycomb), corpuscular type, net type, filter paper type, cotton type, aperture plate type (mesh), sheet type (plate) and template (form) etc. in shape, and there is no particular restriction, and it can be according to suitably distortion and use of the air cleaner of using.Above-mentioned filter can be used as deodorize filters such as active carbon filter, the high-performance filter (HEPA filter) that uses in the multiple household appliances such as being applied in refrigerator or air-conditioner, and use, or use in the lump with above-mentioned each filter as the filters such as air cleaner of automobile.And the object among the present invention is except air cleaning filter, also applicable in the water-purifying filter.
In with the process of pickle lactobacillus fermentation coating on object, pickle lactobacillus fermentation, nano metal particles and binding agent are mixed, then above-mentioned mixed solution is injected in object surfaces by spraying (spray) mode, or object is immersed in the container that fills above-mentioned mixed solution.As long as can make pickle lactobacillus fermentation, nano metal particles and the binding agent can be with coating at body surface, the manufacture method of above-mentioned mixed solution there is no special qualification.The percentage by weight of above-mentioned pickle lactobacillus fermentation preferably 5～20%; But be not to be defined in this, it also can as required and suitably be regulated.And above-mentioned nano metal particles content is preferably the scope below the 100ppm to 2000ppm, but be not be defined in this and can be as required and suitably regulate.In addition, also there is no special qualification on the content ratio of pickle lactobacillus fermentation, nano metal particles and binding agent and can be as required and suitably regulate.
As another kind of mode, above-mentioned coating procedure can carry out coating operation successively by following steps: at first, with coating nano metal particles on the body surface, then the pickle lactobacillus fermentation coating is had on the object of silver in above-mentioned coating.The step of coating nano metal particles can be carried out according to the usual way of this area on the above-mentioned body surface, and can nano metal particles be converted into the state that is suitable for coating according to the characteristic of object as required.And, the pickle lactobacillus fermentation coating there is the step on the object of nano metal particles also there is no special qualification in above-mentioned coating, can use normally used in the art method.For the pickle lactobacillus fermentation coating is had in above-mentioned coating on the object of nano metal particles, can use chemistry, physical method, and use suitable method according to purposes.At this moment, if can be on body surface coating uniformly, can use any methods such as injection, immersion.
In addition, can add following steps as the step before the coating: with the suitable clean target object of clean washing, and by above-mentioned clean target object of oven dry such as heat treatment operations.And, can add following steps as the step after the coating: dry by heat treatment operation etc.Under the situation of oven dry object, its drying time and baking temperature can be regulated according to the shape of the object that uses, kind, size etc.And according to circumstances,, be preferably in and remove upward adherent oil formulation of its surface in its manufacture process or the keeping for objects such as metals.
Below, will the embodiment among the present invention be described in detail, but the present invention is defined in embodiment as described below.
Embodiment 1
To be immersed in from the alum gate net that air filter company buys the 2.5%NaOH about 3 minutes, and the remover oil component, then, the alum gate net of above-mentioned remover oil component to be cleaned with 2.5%NaOH, aforesaid operations carries out 7 times repeatedly.Then, the above-mentioned alum gate net that obtains cleaning is placed in the oven dry baking box that is adjusted to 40 ℃ of temperature, carries out 2 hours heat treatments.Simultaneously, pickle lactobacillus fermentation, nanometer silver and modification silicon acroleic acid binding agent are mixed, and make mixed solution, above-mentioned mixed solution is sprayed carry out coating on the net then at above-mentioned heat treated alum gate, consequently, with obtaining coating the alum gate net of pickle lactic acid bacteria is arranged.
Experimental example 2
In order to detect the coating of making as mentioned above the anti-microbial property of the air cleaning filter of pickle lactobacillus fermentation to coliform, staphylococcus aureus and bacillus pyocyaneus arranged, its sterilizing power is tested according to the method for KS M 0146-2003.
Promptly, utilize assay devices such as high pressure sterilization device, antibacterial culturing thermostat, sterile board, colonometer (ColonyCounter), thermostatic water bath, extinction photometer, the bacterial strain of coliform (Escherichia coliATCC 25922), staphylococcus aureus (Staphylococcus aureus ATCC 6538) and bacillus pyocyaneus (Pseudomonas aeruginosa ATCC 27853) has been carried out the sterilizing power test respectively.Above-mentioned bacterial strains is coated in (each 1.0ml) on the test test portion of the alum gate web filter 1.0 * 1.0cm size among the present invention who obtains by manufacturing, simultaneously with 3 contrast test portions of an inoculation test bacterium liquid, 3 test test portions are positioned in the conical flask (Erlenmeyer flask), and cultivate 1 hour, 2 hours, 3 hours under 35 ± 1 ℃, the condition of 120RPM.Then, extract above-mentioned bacterium liquid, and utilize normal saline to dilute, and under 37 ℃ temperature conditions, in dull and stereotyped incubator, cultivated 48 hours, confirm colony counts, its result with reference to as following table 1 to shown in the table 3.
Table 1: to the sterilizing power of coliform
| Incubation time | ||||
| 0 | After 1 hour | After 2 hours | After 3 hours | |
| The contrast test portion | 1.5×10 5CFU/ml | 1.6×10 5CFU/ml | 1.7×10 5CFU/ml | 2.0×10 5CFU/ml |
| The alum gate web filter | 1.5×10 5CFU/ml | ＜10CFU/ml | ＜10CFU/ml | ＜10CFU/ml |
| 99.9 | 99.9 | 99.9 |
Table 2: to the sterilizing power of staphylococcus aureus
| Incubation time | ||||
| 0 | After 1 hour | After 2 hours | After 3 hours | |
| The contrast test portion | 1.3×10 5CFU/ml | 1.5×10 5CFU/ml | 1.8×10 5CFU/ml | 2.2×10 5CFU/ml |
| The alum gate web filter | 1.3×10 5CFU/ml | ＜10CFU/ml | ＜10CFU/ml | ＜10CFU/ml |
| 99.9 | 99.9 | 99.9 | ||
Table 3: to the sterilizing power of bacillus pyocyaneus
| Incubation time | ||||
| 0 | After 1 hour | After 2 hours | After 3 hours | |
| The contrast test portion | 1.2×10 5CFU/ml | 1.4×10 5CFU/ml | 1.7×10 5CFU/ml | 2.2×10 5CFU/ml |
| The alum gate web filter | 1.2×10 5CFU/ml | ＜10CFU/ml | ＜10CFU/ml | ＜10CFU/ml |
| 99.9 | 99.9 | 99.9 | ||
According to aforesaid table 1 to table 3 as can be known, the air cleaning filter that comprises pickle lactic acid bacteria among the present invention will significantly improve the bactericidal effect to microorganisms such as coliform, staphylococcus aureus, bacillus pyocyaneus.
Be suitable for example
Method of the present invention is applicable to various uses as described below, object by method manufacturing of the present invention is the multiple household appliances that need possess anti-microbial property, as: refrigerator, washing machine, air-conditioner, vacuum cleaner, air purifier, water purifier, humidifier, dish-washing machine, microwave oven, VTR, computer, TV etc.
1) application in refrigerator
Can be applicable to inwall, cool-air duct, blowing fan, door handle of refrigerator, vegetables box, air cleaning filter, ice maker, the carriage of refrigerator, surface of refrigerator outside etc.
2) application in washing machine
But coating and can be applicable to filter (air cleaning filter, water-purifying filter) in dry stream, plumbing stream and the circulation stream on the surface of washing machine structure parts such as inside groove, water jacket, vibrosieve, detergent tube, cylinder lifting device, washing tub.
3) application in air-conditioner
Can be applicable to cooling pin (pin), electrical heat exchanger (ventilation element), blowing fan (metal, injected material), air flow circuit (suction portion, discharge portion, shutter, grid etc.) in the heat exchanger, and the air cleaning filter of indoor set.
4) effect in air purifier
Can in the multi-filter of air purifier, install in addition and contain the pickle lactobacillus fermentation filter.
5) application in vacuum cleaner
Under the situation of vacuum cleaner, situation about touching owing to directly catching is more, therefore can carry out coating to the surface of vacuum cleaner.And can carry out coating to the inside of dust tube and handle, thereby in the dust tube, obtain further bactericidal effect; In addition, also can be applicable in the discharge filter of vacuum cleaner.
6) application in dish-washing machine
Can be applicable to wash the dishes the tube (turb), a water leg (sump), adopt in dirty net, spray arm (spray arm), the water-purifying filter etc.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, every foundation technical spirit of the present invention all still belongs in the scope of technical solution of the present invention any simple modification, equivalent variations and modification that above embodiment did.
The effect of invention
The method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface of the present invention, utilize the have wider antimicrobial spectrum pickle lactobacillus fermentation of (Antibacterial Spectrum), thereby can give full play to the anti-microbial property of household appliances object. And, by above-mentioned pickle lactobacillus fermentation and two kinds of compositions of nano metal particles, can strengthen antimicrbial power. Simultaneously, the antiviral property by pickle lactic acid bacteria has can reach to the virus that causes bird flu stronger safely and effectively antiviral effect.
Claims (13)
1, a kind of method of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface is characterized in that:
With pickle lactobacillus fermentation, binding agent and nano metal particles simultaneously or successively coating on object surfaces.
2, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that: above-mentioned object is household appliances such as refrigerator, washing machine, air-conditioner, air purifier, dish-washing machine.
3, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 2 is characterized in that: above-mentioned object is the internal structure external structure parts of household appliances.
4, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, it is characterized in that: above-mentioned object is a filter.
5, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that: above-mentioned nano metal particles is the metallic with sterilizing function.
6, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 5, it is characterized in that: above-mentioned metallic is the particle of silver, zinc, copper, platinum, ferrum, cadmium, palladium, rhodium, chromium, and it can use separately or use as the alloy more than 2 kinds.
7, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that: above-mentioned binding agent is from by the mixture of selecting a resin or several resins modification silicon acrylic resin, polyurethane resin, acrylic resin and the silicones arbitrarily.
8, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1 is characterized in that: above-mentioned pickle lactobacillus fermentation is selected from the culture fluid of pickle lactic acid bacteria, the concentrated solution of culture fluid, dry thing of culture fluid and composition thereof.
9, according to claim 1 or the 8 described methods of utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface, it is characterized in that: above-mentioned pickle lactic acid bacteria is from by selecting Leuconostoc pickle lactic acid bacteria, Lactobacillus pickle lactic acid bacteria, Wei Si Bordetella pickle lactic acid bacteria and composition thereof.
10, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, it is characterized in that: in above-mentioned coating step, the mixed solution of pickle lactobacillus fermentation, nano metal particles and binding agent is sprayed at body surface, or object is immersed in the above-mentioned mixed solution.
11, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 10 is characterized in that: the percentage by weight of pickle lactobacillus fermentation in above-mentioned mixed solution is 5～20%.
12, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 10 is characterized in that: the content of nano metal particles is 100ppm to 2000ppm in the above-mentioned mixed liquor.
13, the method for utilizing pickle lactobacillus fermentation to impart antibiotic activity on object surface according to claim 1, it is characterized in that: in above-mentioned coating step, with above-mentioned nano metal particles at first coating on body surface, subsequently the pickle lactobacillus fermentation coating is had in above-mentioned coating on the object of nano metal particles, carry out coating operation successively.
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| CN 200610014186 CN101085368A (en) | 2006-06-08 | 2006-06-08 | Method for endowing antibacterial performance to object surface by pickle lactobacillus leavening |
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| CN 200610014186 CN101085368A (en) | 2006-06-08 | 2006-06-08 | Method for endowing antibacterial performance to object surface by pickle lactobacillus leavening |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102697806A (en) * | 2012-05-17 | 2012-10-03 | 中国科学院长春应用化学研究所 | Biological antibacterial agent, as well as preparation method and application thereof |
-
2006
- 2006-06-08 CN CN 200610014186 patent/CN101085368A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102697806A (en) * | 2012-05-17 | 2012-10-03 | 中国科学院长春应用化学研究所 | Biological antibacterial agent, as well as preparation method and application thereof |
| CN102697806B (en) * | 2012-05-17 | 2014-06-18 | 中国科学院长春应用化学研究所 | Biological antibacterial agent, as well as preparation method and application thereof |
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