CN103751156A - Piceatannol-containing composition, and drinking food and cosmetics containing piceatannol-containing composition - Google Patents
Piceatannol-containing composition, and drinking food and cosmetics containing piceatannol-containing composition Download PDFInfo
- Publication number
- CN103751156A CN103751156A CN201310312550.2A CN201310312550A CN103751156A CN 103751156 A CN103751156 A CN 103751156A CN 201310312550 A CN201310312550 A CN 201310312550A CN 103751156 A CN103751156 A CN 103751156A
- Authority
- CN
- China
- Prior art keywords
- piceatannol
- solvent
- seed
- extract
- compositions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
A purpose of the present invention is to provide a high piceatannol content piceatannol-containing composition derived from natural materials, and a preparation method thereof. According to the present invention, the piceatannol-containing composition containing 0.0001-99.9% by mass of piceatannol is extracted from passion fruit seeds; and during extraction of the piceatannol, after the passion fruit seeds are crushed, at least a solvent selected from a water-containing alcohol solvent or a water-containing ketone solvent is added to stir, the piceatannol is extracted from the passion fruit seeds contained in the solvent, and stage extraction is performed by preferentially using the two solvents such as the water-containing alcohol solvent and the water-containing ketone solvent.
Description
Technical field
The present invention relates to a kind of from passionfruit (passion fruit) seed contain piceatannol containing piceatannol compositions and contain this drink foods and cosmetics containing piceatannol compositions.
Background technology
In following non-patent literature 1, recorded and from the polyphenol compound resveratrol (Resveratrol) of Fructus Vitis viniferae and piceatannol (Piceatannol), there is check melanin cellulation and generate melanic effect.But what the validation test of its action effect was used is commercially available reagent, and do not record for obtain the compositions that contains piceatannol etc. from the natural goodses such as Fructus Vitis viniferae.
In addition, in following patent documentation 1, recorded the concentration and recovery that carries out at an easy rate selective and effective resveratrol from the extract of Rhizoma Polygoni Cuspidati (Reynoutria japonica Houtt.var.japnica) root; In following patent documentation 2, recorded the Pericarpium Citri tangerinae extract that contains resveratrol; In following patent documentation 3,4, record the extract of the peel of the extract, particularly passionfruit of passionfruit (Passifloraceae, passiflora sp.), and recorded its extract in food, dietary supplement and use medically.
But the technology of above-mentioned document is all the compositions obtaining from natural goods, and the material that contains higher piceatannol can not be provided.
Look-ahead technique document
Patent documentation
Patent documentation 1: Japanese Unexamined Patent Publication 2005 ?No. 281179 communiques
Patent documentation 2: Japanese Unexamined Patent Publication 2009 ?No. 13159 communiques
Patent documentation 3: Japanese Unexamined Patent Publication 2007 ?No. 302659 communiques
Patent documentation 4: Japanese Patent announce 2007 ?No. 531734 communiques
Non-patent literature
Non-patent literature 1:Takako Yokozawa and You Jung Kim, Piceatannol Inhibits Melanogenesis by Its Antioxidative Actions, Biological & PharmaceuticaI Bulletin Vol.30 (2007) No.11p2007 ?2011
From the general concept of related food safety, be added on the piceatannol in the diet product such as functional food and health food, preferably from natural goods.But, to live us, do not find the natural goods that contains abundant piceatannol, in addition, from the natural goodses such as Fructus Vitis viniferae, only can obtain the low-down compositions of amount.
Therefore, the object of the invention is to, provide a kind of from natural goods, contain higher piceatannol containing piceatannol compositions and contain this drink foods and cosmetics containing piceatannol compositions.
Summary of the invention
The present inventor is for achieving the above object, and through research with keen determination, finds in the past because having no to contain abundant piceatannol in passionfruit seed that purposes goes out of use, thereby completed the present invention.
That is, of the present invention is to obtain from passionfruit seed containing piceatannol compositions, and the amount that it is characterized in that being greater than 10 to 99.9 quality % contains piceatannol.
By of the present invention, containing piceatannol compositions, can provide from compositions natural goods, that contain piceatannol with higher amount.And, owing to being compositions from natural goods, thereby can use it in the diet product of cosmetics, pharmaceuticals and functional food, health food etc.
In addition, preparation method containing piceatannol compositions of the present invention is characterised in that, after passionfruit seed is pulverized, adding is that solvent and moisture ketone are that at least one solvent of selecting solvent stirs from aqueous alcohol, the passionfruit seed containing, extracts piceatannol from this solvent.
By the preparation method containing piceatannol compositions of the present invention, when extracting piceatannol from passionfruit seed, owing to using aqueous alcohol, be that solvent or moisture ketone are solvent, thereby can prepare from compositions natural goods, that contain piceatannol with higher amount with higher production rate.
Of the present invention, contain in the preparation method of piceatannol compositions, after passionfruit seed is pulverized, adding aqueous alcohol is that solvent and moisture ketone are that any solvent in solvent stirs, the passionfruit seed containing from this solvent, extract piceatannol and obtain the 1st extract, again in the residue not reclaiming as described extract, adding aqueous alcohol is that solvent and moisture ketone are that above-mentioned unselected solvent in solvent stirs, the passionfruit seed containing from this solvent, extract piceatannol and obtain the 2nd extract, subsequently described the 1st extract and the 2nd extract are mixed.In this way, use multiple solvent to carry out interim extraction, thus can with the preparation of higher production rate contain a large amount of piceatannols containing piceatannol compositions.
Of the present invention containing in the preparation method of piceatannol compositions, described passionfruit seed preferably the life of passionfruit seed or by the seed after the seed drying of the life of passionfruit.
In addition, of the present invention, containing in the preparation method of piceatannol compositions, described aqueous alcohol is that solvent is preferably aquiferous ethanol, or described moisture ketone is that solvent is preferably aqueous acetone.About its moisture content, aquiferous ethanol is preferably the ethanol that contains 20～99.9 volume %; Aqueous acetone is preferably the acetone that contains 20～99.9 volume %.By such form, can improve the extraction efficiency of piceatannol.
Of the present invention, containing in the preparation method of piceatannol compositions, described stirring in each solvent, preferably carries out with ultrasonic vibration or vibration.By this form, can improve the extraction efficiency of piceatannol.
In addition, of the present invention, containing in the preparation method of piceatannol compositions, after extraction, preferably by distilling under reduced pressure, solvent is removed.
In addition, the invention still further relates to a kind of diet product, wherein added from passionfruit seed, obtain, with the amount of 0.0001～99.9 quality % contain piceatannol containing piceatannol compositions.
In addition, the invention still further relates to a kind of cosmetics, wherein added from passionfruit seed, obtain, be greater than the amount of 10 to 99.9 quality % contain piceatannol containing piceatannol compositions.
By of the present invention, containing piceatannol compositions, can provide from compositions natural goods, that contain piceatannol with higher amount.And, owing to being compositions from natural goods, thereby can be applied in the diet product such as cosmetics, pharmaceuticals and functional food, health food.In addition, by the preparation method containing piceatannol compositions of the present invention, as the solvent that extracts piceatannol from passionfruit seed, what use is that aqueous alcohol is that solvent or moisture ketone are solvent, thereby can prepare from compositions natural goods, that contain a large amount of piceatannols with higher production rate.In addition, can be that solvent or moisture ketone are that the multiple solvent of solvent carries out interim extraction by belonging to aqueous alcohol, thereby be prepared with higher production rate.
Accompanying drawing explanation
Fig. 1 is the chart that represents the HPLC chromatograph of the extract extracting from seed (A) peel (B) and sarcocarp (C);
Fig. 2 is near the MS spectrogram (A) of composition and the chart of UV spectrogram (B) of stripping 41 points during the HPLC that is illustrated in Fig. 1 analyzes;
Fig. 3 represents the MS spectrogram (A) of resveratrol (Resveratrol, molecular weight 228) and the chart of UV spectrogram (B);
Fig. 4 represents the MS spectrogram (A) of piceatannol (Piceatannol, molecular weight 244) and the chart of UV spectrogram (B);
Fig. 5 is the chart of the impact of the extraction efficiency of moisture content on piceatannol that represent ethanol (A) or acetone (B).
The specific embodiment
Of the present invention is the compositions obtaining from passionfruit seed containing piceatannol compositions, with the amount of 0.0001～99.9 quality %, contains piceatannol.Its shape can be aqueous, solid shape, half shape, gel etc. admittedly, in addition, passionfruit seed can be this seed itself and seed is pulverized after pastel or by the seed machining object of the powder after seed lyophilizing pulverizing etc.The solid-state conversion amount of piceatannol, can be 0.0001～99.9 quality %, more preferably 0.01～50 quality %, it would be desirable 0.1～10 quality %.
Piceatannol (Piceatannol) is expressed with following chemical formula (1).
[changing 1]
The piceatannol that upper art chemical formula is expressed, it is the structure that 2 phenyl connect with trans thiazolinyl, it is trans piceatannol, but in the present invention, the concept of piceatannol (Piceatannol) is in above-mentioned chemical formula, also to comprise the cis piceatannol of 2 phenyl with the structure of the thiazolinyl connection of cis.
Passionfruit is the plant of Passifloraceae Passiflora, another name: Passiflora edulis sims, fruit sweet and sour taste, can be used as fruit directly edible, also its squeezing juice can be drunk, or be added in the diet product such as fruit jelly, cake and increase taste.The seed of passionfruit is harder, when squeezing juice etc., it need to be removed from fruit, even the seed of fruit is together added in diet, also can be difficult to because its crust is really up to the mark digestion and directly be drained.The present invention is the invention on the basis of finding in the past because containing abundant piceatannol in having no passionfruit seed that purposes goes out of use.In addition, the passionfruit seed using in the present invention, can be proved by the experience of edible passionfruit in the past, even if be added in the diet product such as functional food and health food, also can not have the problem of safety.
Of the present invention containing piceatannol compositions, preferably, by after passionfruit seed pulverizing for example life or dried, use the method that Different solution extracts to obtain.In order to improve extraction efficiency, also can suitably to passionfruit seed, implement to extract again after the chemical treatments such as acid or alkali decomposition, enzyme decomposition.In addition, by the preparation method containing piceatannol compositions of the present invention of following explanation, can obtain the compositions that contains a large amount of piceatannols with higher production rate, therefore preferably use the method to obtain.
Below the preparation method containing piceatannol compositions of the present invention is described.
Of the present invention, containing in the preparation method of piceatannol compositions, first passionfruit seed is pulverized.Passionfruit seed can be used raw or dried passionfruit seed, in order to improve extraction efficiency, also can suitably to passionfruit seed, implement to extract after the chemical treatments such as acid or alkali decomposition, enzyme decomposition again.Pulverization process can be used general pulverizer to carry out.In addition, because oil content in passionfruit seed is more, may be in the pasty state after pulverizing.
Subsequently, in the passionfruit seed after pulverizing, adding is that solvent and moisture ketone are that at least one solvent of selecting solvent stirs from aqueous alcohol, from this solvent, extracts piceatannol.
Aqueous alcohol is that solvent can be used the aqueous vehicle such as ethanol, methanol, propanol, wherein preferably uses aquiferous ethanol.In addition, moisture ketone is that solvent can be used the aqueous vehicle such as acetone, butanone, diethyl ketone, chlroacetone, wherein preferably uses aqueous acetone.
About its moisture content, aquiferous ethanol can be the ethanol that comprises 20～99.9 volume %, more preferably comprises the ethanol of 40～99.9 volume %, it would be desirable the ethanol that comprises 60～80 volume %; Aqueous acetone can be the acetone that comprises 20～99.9 volume %, more preferably comprises the acetone of 40～99.9 volume %, it would be desirable the acetone that comprises 60～80 volume %.If exceed above-mentioned scope, may cause extraction efficiency to decline.In addition, for the moisture content statement of aqueous vehicle is simplified, below the 80 volume % ethanol of for example moisture 20 volume % are expressed as to " 80% aquiferous ethanol ", the 70 volume % ethanol of for example moisture 30 volume % are expressed as to " 70% aquiferous ethanol ", more for example the 70 volume % acetone of moisture 30 volume % are expressed as to " 70% aqueous acetone ".
Of the present invention, containing in the preparation method of piceatannol compositions, the stirring in above-mentioned each solvent, is preferably used ultrasonic vibration or vibration to carry out.Can allow like this passionfruit seed of having pulverized and contact with above-mentioned each solvent effectively vibrate, thereby can improving from passionfruit seed to above-mentioned each solvent, extract the extraction efficiency of piceatannol.Ultrasonic vibration can be used general ultrasonic unit to carry out.In addition, vibration also can be used general oscillation device to carry out.
In addition, of the present invention, containing in the preparation method of piceatannol compositions, after extraction, preferably use distillation under vacuum to remove above-mentioned solvent.In compositions, just can not comprise organic solvent like this, as the food material adding, can meet the standard of its safety etc. in the diet product such as functional food and health food.
Of the present invention, containing in the preparation method of piceatannol compositions, can carry out interim extraction by multiple solvent.Like this can with the preparation of higher production rate contain a large amount of piceatannols containing piceatannol compositions.
Specifically, for example, after passionfruit seed being pulverized, any solvent that adds described aqueous alcohol and be solvent and described moisture ketone and be in solvent stirs, and extracts piceatannol and obtain the 1st extract from this solvent.By the mode such as centrifugal by extract with not to be used as the residue that extract reclaims Fen Lis, in residue, add non-selected another kind of solvent in described solvent to stir, from this solvent, extract piceatannol acquisition the 2nd extract.Subsequently described the 1st extract and the 2nd extract are mixed.Certainly, the 2nd extract also can utilize as the extract of passionfruit seed separately.
In addition, passionfruit seed is that solvent or described moisture ketone are the 1st extraction process of solvent owing to having accepted by described aqueous alcohol, the characteristic of its structure etc. becomes more suitable because of extraction, so when ensuing the 2nd extraction process, no matter using described aqueous alcohol is that solvent or described moisture ketone are solvent, still use other solvent in addition, all can expect that its extraction efficiency improves.And, as described later described in embodiment, described aqueous alcohol is that the extraction efficiency that solvent or described moisture ketone are solvent is moisture based on it, so passionfruit seed is carried out after the 1st extraction process at water or water solublity solvent, when ensuing the 2nd extraction process, no matter using described aqueous alcohol is that solvent or described moisture ketone are solvent, still use other solvent in addition, all can expect that its extraction efficiency improves.
The extract of the passionfruit seed obtaining by said method, can be directly or the liquid material being concentrated as containing piceatannol compositions.Also can be dry by its powdered by lyophilization or spraying, using pulverous material as containing piceatannol compositions.In addition, the invention is not restricted to these forms.Insoluble matter contained in extract suitably can be removed by modes such as filtrations, also insoluble matter can be pulverized again, form minuteness particle shape.
In addition, for the first extract of the passionfruit seed obtaining as stated above, can be by methods such as ion exchange, size exclusion chromatography (SEC), HPLC method, gel filtration, membrance separation, by piceatannol by index carry out classification, refining after, set it as containing piceatannol compositions.
Specifically, for example, shown in enforcement described later, the compositions of extracting is used to HPLC method under the following conditions from passionfruit seed, piceatannol is dissolved near 41 points.In addition, this stripping part is carried out to mass spectrum (mass spectrum) by quality analysis apparatus and measure, the signal of negative ion mode is m/z243.
[HPLC condition]
Post: Mightysil RP ?18GP150 ?4.6 footpath 4.6mm, long 150mm(Kanto Kagaku K. K.)
Column temperature: 40 ℃
Leaching condition: flow velocity 1ml/min, 0% methanol → 30% methanol (60min)
UV detects: 280nm
Like this, in the present invention, take the physical property of the piceatannol of above-mentioned example as index, carry out suitable classification, refining, thus can prepare improved piceatannol amount, from passionfruit seed containing piceatannol compositions.
Of the present invention containing piceatannol compositions, can add as required substrate or carrier that materia medica is allowed, form the form of tablet, granule, powder, liquor, powder, granule, capsule, gluey agent etc. for medicine, in addition, also can form the forms such as ointment, cream, gel, facial film, astringent, moisturizer for cosmetics.
In addition, of the present invention containing piceatannol compositions, also can be added into specific food for health care, dietary supplement, functional food etc. for picked-up.Such food for example has the pastry food of chocolate, cookies, chewing gum, confection, cookie, fruit jelly, ship biscuit etc.; Cereals; The beverage of powder drink, refreshment drink, milk beverage, nutritious drink, soda pop, gel beverage etc.; The freezing point of ice cream, ice cream etc.In addition, the Noodles of Fagopyrum esculentum Moench, wheaten food, noodles, fine dried noodles etc. is also good example.In addition, specific food for health care and dietary supplement etc. can be the forms of powder, granule, capsule, syrup, tablet, sugar-coat agent etc.
Embodiment
Below by embodiment, the present invention is specifically described, but scope of the present invention is not limited with these embodiments.
The detection of the piceatannol in < test example 1>[passionfruit and same fixed]
Passionfruit is divided into peel, sarcocarp, 3 positions of seed, carries out respectively lyophilization, after pulverizing with pulverizer (trade name " samplemil pattern SK ?M ", Xie Li science and engineering Co., Ltd.), each position is extracted with 80% aquiferous ethanol.After the heart far away, get the supernatant, after concentrating with vaporizer, carry out lyophilization and form powder.By water-soluble this powder extract that is.Subsequently, each solid-state concentration is diluted, seed extract is diluted to 0.01g/ml, and peel extract or pulp extract are diluted to 0.1g/ml, and respectively gets 5 μ l and carry out HPLC analysis.
The analysis condition of HPLC is as follows:
Post: Mightysil RP ?18GP150 ?4.6 footpath 4.6mm, long 150mm(Kanto Kagaku K. K. system)
Column temperature: 40 ℃
Leaching condition: flow velocity 1ml/min, 0% methanol → 30% methanol (60min)
UV detects: 280nm
What Figure 1A～Fig. 1 C represented is the HPLC chromatograph of above-mentioned each extract.As shown in Figure 1A, seed extract reaches peak to its result near 41 points.In contrast to this, the extract of peel and sarcocarp, although the solid-state concentration of injecting is 10 times of seed extract, near peak unconfirmed 41 points.
Next, seed extract is near the composition of stripping 41 points of above-mentioned retention times what material carries out following same fixed.
First, use HPLC/ quality analysis apparatus (THERMO ELECTRON Co., Ltd. system, device name " Thermo ?LCQ ?MS/MS ") to measure its mass spectrum (Fig. 2 A).By anode, two kinds of ion modes of negative electrode, confirm, at negative electrode, found good ion, so use its data.Consequently, main signal m/z243 is (M ?H)
?, other peak m/z279 belongs to (M+2H
2o ?H)
?, m/z487 belongs to (M × 2 ?H)
?.Therefore determined that molecular weight is 244.In addition, get UV spectrum, as λ max, observe 220nm, 304nm, 322nm(Fig. 2 B).
The result of these mass spectrums and UV spectrum, with contained polyphenol---the resveratrol (Resveratrol, molecular weight 228) similar (with reference to Fig. 3 A, B) of peanut skin.In addition, also confirmed separately to the absorption of the resin polyvinylpyrrolidone (PVPP:polyvinylpolypyrrolidone) of adsorbing polyphenols.In addition, near the peak above-mentioned 41 points was compared with the retention time of the resveratrol under identical HPLC condition, shorter to the retention time of reversed-phase column.In sum, above-mentioned seed extract is near the composition of stripping 41 points of retention times, is than piceatannol (Piceatannol, the molecular weight 244) hydroxyl of large 1 molecular weight of resveratrol, shown in following formula (1).
[changing 2]
Therefore, the pure compound (SIGMA company system) to piceatannol has carried out HPLC analysis under condition same as described above, again obtains mass spectrum and UV spectrum (Fig. 4 A, B).Consequently, in full accord in the retention time of HPLC and the ownership of mass spectrum and UV spectrum.
Can confirm thus, near the peak in above-mentioned HPLC analyzes 41 points of above-mentioned seed extract is the peak of piceatannol, contains a large amount of piceatannols in the seed of passionfruit.In addition, in the peel of passionfruit and sarcocarp, do not detect piceatannol.
< test example 2>[extracts piceatannol 1 from passionfruit seed]
We are studied the method for extracting piceatannol from passionfruit seed.Specifically use different solvents, the efficiency of extracting piceatannol from the seed of domestic product and external 2 kinds of passionfruits that produce is confirmed.
(1) extraction of use 80% aquiferous ethanol
In the seed of pulverizing at 5g, add 80% aquiferous ethanol 50ml, at 30 minutes, in ultrasonic vibration, extract.Reclaim after its supernatant, repeat 2 same operations, carry out altogether 3 times and extract.Subsequently the extracting solution reclaiming is concentrated, the extracting solution of acquisition is settled to 200ml with 80% aquiferous ethanol, its 10 μ l is carried out to HPLC analysis.Subsequently, by common method, the peak of analyzing from HPLC, area starts, and sets it as the slotted line of standard substance concentration, thereby the amount of piceatannol contained in extract is carried out quantitatively.In addition, in order to carry out reference, quantitative too to resveratrol.
(2) extraction of use 70% aqueous acetone
In extraction residue after above-mentioned use 80% aquiferous ethanol extracts, add 70% aqueous acetone 50ml, at 30 minutes, in ultrasonic vibration, extract.Reclaim after its supernatant, repeat 2 same operations, carry out altogether 3 times and extract.Subsequently the extracting solution reclaiming is concentrated, the extracting solution of acquisition is settled to 200ml with 70% aqueous acetone, with above-mentioned same, the amount of piceatannol contained in extract and resveratrol is carried out quantitatively.
(3) extraction of use ethyl acetate
Solvent is replaced by ethyl acetate, same with the extraction of above-mentioned use 80% aquiferous ethanol, the seed of passionfruit (above-mentioned outer domestic) is extracted, the piceatannol extracting in the 5g seed powder from lyophilization and the amount of resveratrol are carried out quantitatively.
(4) extraction of use dichloromethane (dichloromethane)
Solvent is replaced by dichloromethane, same with the extraction of above-mentioned use 80% aquiferous ethanol, the seed of passionfruit (above-mentioned outer domestic) is extracted, the piceatannol extracting in the 5g seed powder from lyophilization and the amount of resveratrol are carried out quantitatively.
By result arrange after as described in Table 1.In addition, in this table 1, the result of extraction efficiency, is that the amount of piceatannol or the amount of resveratrol of extracting in the 100g seed powder being scaled from lyophilization represents.
[table 1]
As shown in table 1, even in the extraction residue using after 80% aquiferous ethanol extracts, compared with using the extraction of 80% aquiferous ethanol, still extracted more piceatannol.In addition, use separately the extraction efficiency of ethyl acetate lower, while using separately dichloromethane to extract, fail to detect piceatannol and resveratrol.Hence one can see that, uses 70 efficiency of extracting containing acetone best.Can also know in addition, by using the extraction of 80 aquiferous ethanol and extraction the use of 70% aqueous acetone, can obtain better extraction efficiency.
< test example 3>[extracts piceatannol 2 from passionfruit seed]
We have carried out further research to the method for extracting piceatannol from passionfruit seed.Specifically the moisture content of the ethanol as solvent and acetone is confirmed the impact of extraction efficiency.
For this reason, we use the ethanol of different water cut or acetone as solvent, same with above-mentioned test example 2, and the piceatannol extracting from the 5g seed powder after passionfruit (domestic above-mentioned) lyophilization and the amount of resveratrol are carried out quantitatively.In addition, the solvent ethyl acetate and the dichloromethane that in above-mentioned test example 2, use, because the intermiscibility of itself and water is lower, all fail to modulate the solvent of same moisture content.
By result arrange after as described in Table 1.In addition, in Fig. 5, the result of piceatannol is organized into chart.In addition, in this table 2 and Fig. 5, the result of extraction efficiency, is all that the amount of piceatannol or the amount of resveratrol of extracting in the every 100g seed powder being scaled from lyophilization represents.
[table 2]
*: countless certificates
As shown in table 2, Fig. 5, while using ethanol, use the extraction of 80% aquiferous ethanol the most effective.In addition, when using acetone, use the extraction of 70% aqueous acetone the most effective.
< test example 4>
The extract (from above-mentioned outer domestic) after solvent is removed in weighing in above-mentioned test example 2, calculates solid-state piceatannol content.Its value is as shown in table 3.
[table 3]
| Extract solvent | 80 |
70% aqueous acetone |
| Piceatannol content (quality %) in the solid-state part of extracting solution | 2.6 | 3.7 |
As shown in table 3, while using 80% aquiferous ethanol to extract, the solid-state part concentration of piceatannol is 2.6mg/100mg extract (2.6 quality %).In addition, while using 70% aqueous acetone to extract after using 80% aquiferous ethanol to extract, be 3.7mg/100mg extract (3.7 quality %).Therefore, use 70% aqueous acetone to extract and can obtain more piceatannol (as shown in test example 2,3), meanwhile, the purity of piceatannol is also higher.
Claims (3)
1. containing a piceatannol compositions, it is characterized in that:
From passionfruit seed, obtain, with the amount that is greater than 10 to 99.9 quality %, contain piceatannol.
2. diet product, is characterized in that:
Added with the amount of 0.0001～99.9 quality % contain the piceatannol that obtains from passionfruit seed containing piceatannol compositions.
3. cosmetics, is characterized in that:
Added amount to be greater than 10 to 99.9 quality % contain the piceatannol that obtains from passionfruit seed containing piceatannol compositions.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200980156466.8A CN102307837B (en) | 2009-04-03 | 2009-04-03 | Piceatannol-containing composition and method of producing piceatannol-containing composition |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200980156466.8A Division CN102307837B (en) | 2009-04-03 | 2009-04-03 | Piceatannol-containing composition and method of producing piceatannol-containing composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103751156A true CN103751156A (en) | 2014-04-30 |
| CN103751156B CN103751156B (en) | 2015-07-22 |
Family
ID=50518590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310312550.2A Active CN103751156B (en) | 2009-04-03 | 2009-04-03 | Piceatannol-containing composition, and drinking food and cosmetics containing piceatannol-containing composition |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN103751156B (en) |
| HK (1) | HK1192144A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110734361A (en) * | 2019-09-29 | 2020-01-31 | 钟华 | Preparation method and application of piceatannol |
| CN111704530A (en) * | 2020-07-07 | 2020-09-25 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rheum officinale, microcapsule molecule embedding method and application of piceatannol in cosmetics |
| CN111763141A (en) * | 2020-07-07 | 2020-10-13 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rhubarb medicinal materials, microcapsule molecule embedding method and application of microcapsule molecule embedding method in food and health care products |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080118449A1 (en) * | 2006-11-21 | 2008-05-22 | Alvin Ronlan | Cosmetic formulations of piceatannol and retinoids and methods of use thereof |
| WO2008086400A2 (en) * | 2007-01-09 | 2008-07-17 | Isp Investments Inc. | Sirtuin-activating compounds of enhanced bioavailability |
-
2009
- 2009-04-03 CN CN201310312550.2A patent/CN103751156B/en active Active
-
2012
- 2012-05-30 HK HK14105272.2A patent/HK1192144A1/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080118449A1 (en) * | 2006-11-21 | 2008-05-22 | Alvin Ronlan | Cosmetic formulations of piceatannol and retinoids and methods of use thereof |
| WO2008086400A2 (en) * | 2007-01-09 | 2008-07-17 | Isp Investments Inc. | Sirtuin-activating compounds of enhanced bioavailability |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110734361A (en) * | 2019-09-29 | 2020-01-31 | 钟华 | Preparation method and application of piceatannol |
| CN111704530A (en) * | 2020-07-07 | 2020-09-25 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rheum officinale, microcapsule molecule embedding method and application of piceatannol in cosmetics |
| CN111763141A (en) * | 2020-07-07 | 2020-10-13 | 昆明翔昊科技有限公司 | Method for extracting piceatannol from rhubarb medicinal materials, microcapsule molecule embedding method and application of microcapsule molecule embedding method in food and health care products |
Also Published As
| Publication number | Publication date |
|---|---|
| HK1192144A1 (en) | 2014-08-15 |
| CN103751156B (en) | 2015-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102307837B (en) | Piceatannol-containing composition and method of producing piceatannol-containing composition | |
| CN101156670B (en) | A production process of nutrition health care roxburgh rose powder | |
| CN103501640B (en) | Composition containing scirpusin B, and process for producing composition containing scirpusin B | |
| CN107684575A (en) | The preparation method and purposes of white bur activity extract | |
| WO2014185653A1 (en) | Health supplement using dendropanax morbifera lev and method for producing same | |
| CN103751156B (en) | Piceatannol-containing composition, and drinking food and cosmetics containing piceatannol-containing composition | |
| TWI441655B (en) | Composition containing piceatannol and method for preparing same | |
| KR20070089309A (en) | The preparation method of concentrated material of gelidium sp. showing antibacterial, anti-oxidative and antitumor activities and the functional food composition comprising the same | |
| CN104146895A (en) | Piceatannol-containing composition and method for preparing piceatannol-containing composition | |
| JP4688795B2 (en) | Preparation method of banaba extract | |
| JP5637707B2 (en) | Skillpsin B-containing composition and method for producing skillpsin B-containing composition | |
| JP2010229120A (en) | Method for producing maloideae plant leaf extract highly containing phloridzin | |
| JP2001039844A (en) | Gargle for throat | |
| JP2005336165A (en) | Saw palmetto extract composition and method for producing saw palmetto extract | |
| JP2005281271A (en) | Skin moisturizing ability improving agent | |
| JP2006056862A (en) | Deodorizer containing polyisoprene | |
| JP2023003173A (en) | Anti-aging composition | |
| KR20170002074A (en) | Red beverage with anti-aging activity containing juices or extractions of red paprika, beet leaf, and redglobe, and method for preparing the same | |
| CN117264690A (en) | Extraction method of camellia oil rich in polyphenols | |
| CN109665941A (en) | A method of xylitol is extracted from green soy bean shell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1192144 Country of ref document: HK |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: GR Ref document number: 1192144 Country of ref document: HK |