CN103750563A - Preparation method and radical reduction and harm reduction application of squalene and tea polyphenol composition liquid proliposome - Google Patents
Preparation method and radical reduction and harm reduction application of squalene and tea polyphenol composition liquid proliposome Download PDFInfo
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Abstract
The invention discloses a preparation method and radical reduction and harm reduction application of squalene and tea polyphenol composition liquid proliposome. The liquid proliposome is prepared from 7-10 parts of hydrogenated phospholipid, 8-12 parts of stabilizer, 10-15 parts of surfactant, 25-45 parts of squalene and tea polyphenol composition and the balance water. The liquid proliposome is filled into a hollow cylinder with waterproof dustproof gas-permeable membranes at two ends, and the hollow cylinder is disposed inside a cigarette filter in the forming process of a cigarette filter rod to obtain the cigarette filter capable of reducing cigarette harm. When a consumer smokes, smoke passes the internally-disposed liquid proliposome, an antioxidant and main stream smoke are in full contact since the liquid proliposome is large in surface area, gas-phase free radicals in the main stream smoke can be effectively eliminated, and strong technical support is provided for solving the problem about smoking and health.
Description
Technical field
The present invention relates to technical field of chemistry, more specifically, relate to a kind of liquid precursor liposome containing squalene and tea polyphenol composition and preparation method thereof and fall the application aspect base harm reduction at shredded tobacco for water pipes type filter tip.
Background technology
The harm that thousands of kinds of compounds that smoking produces and the radical pair human body of high concentration cause causes people's generally attention.And the effect of radical pair health endangers the most by force maximum, it can cause tuberculosis, heart disease and cancer, and these radical pairs smoker produces serious injury.Smoking produces the free radical of high concentration, comprises the phase Free Radicals that the life-span is relatively grown, and generation is reacted in quinones and half quinones, multiring aromatic hydrocarbon free radical and they active oxygen radical with oxygen is as superoxide anion (O
2 -), hydrogen peroxide (H
2o
2), hydroxy radical (OH), singlet oxygen (
1o
2) and the gaseous phase free radical relatively short with life period such as lipid peroxidation free radical (ROO), alkoxy free group (RO), alkyl diradical (R), nitrogen oxide free radical etc.
At present in to flue gas aspect radicals scavenging research and few, mainly from additional natural, vitamin, amino acid, protein etc. and biology enzyme free radical scavenger and trace element, natural mineral matter, reduce the injury of radical pair human body flue gas.Further, there is the custom of water suction cigarette China some areas, its be cigarette after burning by the water in water pipe after again in suction nozzle, it not only can remove water-soluble harmful chemical, can also remove part gaseous phase free radical, has alleviated the injury of smoking to human body.And can alleviate because smoking causes the probability that human body is got angry.The utility model patent that Luo Fuzhong (2013) is CN 203182005 U at publication number discloses a kind of shredded tobacco for water pipes type filter tip, in water, add surfactant and/or add the Chinese herbal medicines such as peppermint, Radix Glycyrrhizae to extract composition, its object reaching is significantly to reduce tar, nicotine content, can not get angry, also can not affect the taste of tobacco, simple in structure, with low cost; In the utility application that Jiang Zhimin (2008) is ZL200820107184.1 in the patent No., disclose a kind of dipping snuff filter tip, in dipping snuff filter tip structure, improved design.Jiang Huaguo (1998) discloses a kind of Portable water tobacco pipe in its utility application, by simplifying the structure of water tobacco pipe; In the patent of invention that Huang Hui (2005) is CN200510129846.6 at publication number, disclose a kind of method of water tobacco pipe to decoking cigarette and comprehensive harm reduction of utilizing, studied decoking and modulated the liquid formulation of moulding with comprehensive harm reduction.In above-mentioned shredded tobacco for water pipes type filter tip, all do not relate to suitable antioxidant science is added in shredded tobacco for water pipes type cigarette holder, remove the research of gaseous phase free radical in flue gas.
Squalene is a kind of lipid unsaponifiable matter, for the many unsaturated olefins of one (squalene), to find from the liver oil of shark at first, within 1914, be named as Squalene, its chemical name is 2, 6, 10, 15, 19, 23-hexamethyl-2, 6, 10, 14, 18, 22-tetracosa carbon six alkene, belong to open chain triterpene, claim again Squalene, participate in human body cholesterol biosynthesis and can enhance metabolism, there is superoxide dismutase (SOD) activity in the body of raising, strengthen body immunity, anti-ageing, antifatigue, the different physiological roles such as antitumor, it is a kind of avirulent marine bioactivity material with the effect of preventing and curing diseases.Squalene distributes very wide in plant, but content is not high, and many lower than 5% of unsaponifiable matter in vegetable oil, only minority content is more, and as olive oil contains squalene 150～700mg/100g, in rice bran oil, its content is 332mg/100g.
Tea Polyphenols is the general name of Catechin in Tea class, acetone class, phenolic acid class and anthocyan compound.Be a kind of condensed ring aromatic hydrocarbon, can be divided into flavanol compound, hydroxyl-[4]-flavanol compound, anthocyanin class, flavonoids, flavonols and phenolic acid class etc.Wherein important with catechin, account for 60%～80% of Polyphenols total amount; Catechin is mainly by several monomer compositions such as EGC, DLC, EC, EGCG, GCG, ECG.The content of Tea Polyphenols in tealeaves is generally 20～35%.In Tea Polyphenols in each composition part take flavanol compound as main, flavanol compound is again take catechins as main.The content of catechins accounts for 70% left and right of Tea Polyphenols total amount.There is the effect of the harmful free radical of very strong elimination, anti-aging effects, radiation resistance, to the inhibitory action of cancer cell, antibacterial, bactericidal action, to the inhibitory action of AIDS virus, existingly studies have shown that Tea Polyphenols is also beneficial to smoker, can clean lung's nicotine.
In order to overcome the shortcoming of common antioxidant, reach the object that can simultaneously remove long-life phase Free Radicals and the relatively short gaseous phase free radical of life period, the applicant is devoted for years in correlative study and obtains Patents.In the application that is ZL201010529711.X in the patent No., disclose squalene and tea polyphenol composition and the application in cigarette thereof, squalene and Tea Polyphenols synergistic composition have directly been added in pipe tobacco, obtained good effect.But further research is found, squalene and Tea Polyphenols are easy to oxidation deterioration in air, composition is unstable when application, actual production inconvenience.
Liposome (liposome) is a kind of artificial membrane.When amphiphatic molecule is scattered in water as phosphatide and sphingolipid, the hydrophobic tail of molecule is tended to flock together, and avoids water, and hydrophilic head is exposed to water, form have bilayer structure vesicle, be called liposome.According to textural classification, liposome is divided into unilamelar liposome, multilamelar liposome and liquid precursor liposome.Liquid precursor liposome is a kind of liposome with slow release effect, by many non-concentric chamber, is connected and composed, and particle diameter, at 5～50 μ m, has larger envelope volume than conventional liposome, is particularly useful for sealing of water soluble drug.The application of liposome is mainly aspect medical at present.
These free radical scavengers are processed into liquid precursor liposome, and science inserts the research of removing gaseous phase free radical (falling base) in shredded tobacco for water pipes type cigarette holder and have no at present report, more have no technology of preparing and the application report of the liquid precursor liposome based on squalene and Tea Polyphenols synergistic composition.
Summary of the invention
The object of the invention is to overcome the application deficiency of existing liquid precursor liposome technology aspect cigarette, and squalene and Tea Polyphenols synergistic composition are in the technical deficiency of cigarette application aspect, and a kind of liquid precursor liposome containing squalene and tea polyphenol composition is provided.
Another object of the present invention is to provide the preparation method of described liquid precursor liposome.
An also goal of the invention of the present invention is to provide the application of the described liquid precursor liposome containing squalene and tea polyphenol composition in shredded tobacco for water pipes type filter tip.
Object of the present invention is achieved by the following technical programs:
A kind of liquid precursor liposome containing squalene and tea polyphenol composition is provided, and described liquid precursor liposome is to be prepared by each component of following mass fraction:
Hydrogenated phospholipid: 7～10 parts;
Stabilizing agent: 8～12 parts;
Surfactant: 10～15 parts;
Squalene and tea polyphenol composition: 25～45 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts;
Preferably, the described liquid precursor liposome containing squalene and tea polyphenol composition, described liquid precursor liposome is to be prepared by each component of following mass fraction:
Hydrogenated phospholipid: 8～9 parts;
Stabilizing agent: 9～11 parts;
Surfactant: 12～14 parts;
Squalene and tea polyphenol composition: 30～35 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts.
Can adopt multi-emulsion method to prepare the described liquid precursor liposome containing squalene and tea polyphenol composition with above-mentioned each component.
Described phosphatide is one or more in lecithin, soybean lecithin, hydrogenated soya phosphatide; Adopt multiple phosphatide, the mixed proportion of various phosphatide is not done strict restriction.
The preferred hydroxyl starch of described stabilizing agent, more preferably, described hydroxyl starch is one or more in hydroxymethyl starch, HES or hydroxypropul starch.Adopt when multiple, mixed proportion is not done strict restriction.
Described surfactant is glycerine.
In described squalene and tea polyphenol composition, the mass ratio of squalene and Tea Polyphenols is 1: 8～8: 1, and the ratio of more optimizing is: 2: 7～7: 2.
Described squalene can be the extract that extracts from plant, animal or microorganism or purified after sterling, can adopt commercial product.
Described Tea Polyphenols can be that the Tea Polyphenols extract that extracts from tealeaves can be also the sterling after purified, can adopt commercial product.
The present invention provides the preparation method of the described liquid precursor liposome containing squalene and tea polyphenol composition simultaneously, science compatibility raw material, and accurate process conditions, solubility is not in water to have overcome squalene and Tea Polyphenols
High shortcoming, successfully prepares the liquid precursor liposome containing squalene and Tea Polyphenols mixture synergistic composition, can realize well and be applied to the filter-tip object of shredded tobacco for water pipes type.Described preparation method comprises the following steps:
S1. phosphatide and squalene and tea polyphenol composition are dissolved in glycerine, obtain mixed system;
S2. it is fully mixed S1 gained mixed system high speed homogenizing, obtain the mixed system of homogenizing;
S3. stabilizing agent and water are mixed to get to the aqueous solution of stabilizing agent, after adding the aqueous solution of stabilizing agent in the mixed system of S2 gained homogenizing, stir, form stable microparticle suspending liquid and obtain the liquid precursor liposome containing squalene and tea polyphenol composition.
Preferably, described in S2, the process conditions of high speed homogenizing are: 2000 r/min, stirring 45min.
The invention provides the liquid precursor liposome containing squalene and tea polyphenol composition that said method prepares.
Liquid precursor liposome containing squalene and tea polyphenol composition of the present invention obtains well application in cigarette, specifically be applied to shredded tobacco for water pipes type filter tip, be that described liquid precursor liposome is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, in cigarette filter tip rod forming process, be built in the cigarette filter of acetate fiber.
Preferably application process is: described liquid precursor liposome is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, every packs 1 gram of described liquid precursor liposome into, in cigarette filter tip rod forming process, be built in the cigarette filter of acetate fiber, built-in 1 of every cigarette filter, obtain reducing the cigarette filter of cigarette harm, when consumer's smoking, flue gas is by built-in liquid precursor liposome, because liquid precursor surface of liposome is long-pending large, antioxidant fully contacts with main flume, can effectively remove the gaseous phase free radical in main flume, for solving smoking and healthy problem, provide strong technical support.
Beneficial effect of the present invention:
The present invention is applied to the liposome especially technology of liquid precursor liposome in cigarette first, and success is in conjunction with squalene and Tea Polyphenols synergistic composition, sum up suitable compatibility auxiliary agent and proportion compatibility, prepare the liquid precursor liposome containing squalene and Tea Polyphenols synergistic composition simplely, for the further developing application of liposome provides strong technical support, gained liquid precursor liposome is high degree of dispersion fine droplet, decentralization is high, specific area is large, can directly apply in shredded tobacco for water pipes type filter tip.
The present invention is successfully embedded in synergistic composition in liquid precursor liposome, overcome the shortcoming that squalene and Tea Polyphenols are easy to oxidation deactivation in air, overcome squalene and the water-fast defect of Tea Polyphenols synergistic composition simultaneously, by to squalene and Tea Polyphenols mixture synergistic composition microencapsulation, reach the stability that increases squalene and Tea Polyphenols mixture synergistic composition, acquisition can be applied to the liquid precursor liposome of cigarette filter.
The present invention accurately limits material combination and preparation process condition, the liquid precursor liposome gas permeability that processing obtains is strong, surface area is large, be applicable to the application demand of cigarette filter, can directly apply to shredded tobacco for water pipes type filter tip, antioxidant can fully contact with main flume, reaches the object of removing free radical in main flume, for solving smoking and healthy problem, provides strong technical support.
The specific embodiment
Below in conjunction with specific embodiment, further describe the present invention.The test method of using in following embodiment if no special instructions, is conventional method; The material that uses, reagent etc. if no special instructions, are reagent and the material that can obtain from conventional commercial sources.
Embodiment 1
According to following mass fraction, prepare each component: 10 grams of hydrolecithins (g); Squalene (the true Bioisystech Co., Ltd in Shanghai, content is greater than 96.0%, and following embodiment is same.But therefore do not limit the present invention, those skilled in the art can adopt the squalene in other sources) 5 grams (g); Tea Polyphenols (Ningbo You Jie Chemical Co., Ltd., content is greater than 98.0%, and following embodiment is same.But therefore do not limit the present invention, those skilled in the art can adopt the Tea Polyphenols in other sources) 20 grams (g); 10 grams of glycerine (g); 8 grams of hydroxymethyl starches (g), 47 grams, water (g).
Preparation process is:
S1. hydrolecithin and squalene and tea polyphenol composition are dissolved in glycerine, obtain mixed system;
S2. it is fully mixed S1 gained mixed system high speed homogenizing, obtain the mixed system of homogenizing;
S3. stabilizing agent and water are mixed to get to the aqueous solution of stabilizing agent, after adding the aqueous solution of stabilizing agent in the mixed system of S2 gained homogenizing, stir, form stable microparticle suspending liquid and obtain the liquid precursor liposome containing squalene and tea polyphenol composition.
Embodiment 2
Preparation process is with embodiment 1.Take 10 grams of hydrogenated soya phosphatides, 10 grams of squalenes, 15 grams of Tea Polyphenols are dissolved in 10 grams of glycerine, high speed homogenizing fully mixes it, add again 8 grams of HESs to be dissolved in the aqueous solution of 47 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 3
Preparation process is with embodiment 1.Take 10 grams of hydrogenated soya phosphatides, 15 grams of squalenes, 10 grams of Tea Polyphenols are dissolved in 10 grams of glycerine, high speed homogenizing fully mixes it, add again 8 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 47 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 4
Preparation process is with embodiment 1.Take 10 grams of hydrogenated soya phosphatides, 5 grams of squalenes, 20 grams of Tea Polyphenols are dissolved in 10 grams of glycerine, high speed homogenizing fully mixes it, add again 8 grams of hydroxypropul starch to be dissolved in the aqueous solution of 47 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 5
Preparation process is with embodiment 1.Take 8 grams of hydrolecithins, 5 grams of squalenes, 25 grams of Tea Polyphenols are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of HESs to be dissolved in the aqueous solution of 40 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 6
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 10 grams of squalenes, 20 grams of Tea Polyphenols are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxypropul starch to be dissolved in the aqueous solution of 40 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 7
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 15 grams of squalenes, 15 grams of Tea Polyphenols are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 40 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 8
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 20 grams of squalenes, 10 grams of Tea Polyphenols are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of HESs to be dissolved in the aqueous solution of 40 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 9
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 25 grams of squalenes, 5 grams of Tea Polyphenols are dissolved in 12 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxypropul starch to be dissolved in the aqueous solution of 40 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 10
Preparation process is with embodiment 1.Take 7 grams of hydrogenated soya phosphatides, 5 grams of squalenes, 35 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 12 grams of hydroxypropul starch to be dissolved in the aqueous solution of 26 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 11
Preparation process is with embodiment 1.Take 7 grams of hydrolecithins, 10 grams of squalenes, 30 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 12 grams of HESs to be dissolved in the aqueous solution of 26 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 12
Preparation process is with embodiment 1.Take 7 grams of hydrolecithins, 15 grams of squalenes, 25 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 12 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 26 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 13
Preparation process is with embodiment 1.Take 7 grams of hydrolecithins, 20 grams of squalenes, 20 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 12 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 26 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 14
Preparation process is with embodiment 1.Take 7 grams of hydrogenated soya phosphatides, 30 grams of squalenes, 10 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 12 grams of HESs to be dissolved in the aqueous solution of 26 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 15
Preparation process is with embodiment 1.Take 7 grams of hydrolecithins, 35 grams of squalenes, 5 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 12 grams of hydroxypropul starch to be dissolved in the aqueous solution of 26 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 16
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 5 grams of squalenes, 40 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 22 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 17
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 10 grams of squalenes, 35 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of HESs to be dissolved in the aqueous solution of 22 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 18
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 20 grams of squalenes, 25 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxypropul starch to be dissolved in the aqueous solution of 22 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 19
Preparation process is with embodiment 1.Take 8 grams of hydrolecithins, 30 grams of squalenes, 15 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 22 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 20
Preparation process is with embodiment 1.Take 8 grams of hydrogenated soya phosphatides, 35 grams of squalenes, 10 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 22 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
Embodiment 21
Preparation process is with embodiment 1.Take 8 grams of hydrolecithins, 40 grams of squalenes, 5 grams of Tea Polyphenols are dissolved in 15 grams of glycerine, high speed homogenizing fully mixes it, add again 10 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 22 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.
The liquid precursor liposome testing result that above-described embodiment 1～21 is prepared is as follows: adopt laser scattering method to detect, the liquid precursor liposome particle diameter that embodiment 1～21 prepares is 65～100nm, is evenly distributed, and is normality, and span is little.
Adopt dialysis separation method that squalene free in solution is separation with liposome with Tea Polyphenols, respectively mensuration, computational envelope rate.According to (in liposome in squalene and tea polyphenol composition quality/liposome squalene and tea polyphenol composition gross mass) × 100% computational envelope rate.The liquid precursor liposome encapsulation that the embodiment of the present invention 1～21 prepares is more than 88%.
Comparative example 1
Preparation process is with embodiment 1.Take 5 grams of hydrolecithins, 40 grams of squalenes, 5 grams of Tea Polyphenols are dissolved in 20 grams of glycerine, high speed homogenizing fully mixes it, add again 5 grams of hydroxymethyl starches to be dissolved in the aqueous solution of 25 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.The liquid precursor liposome particle diameter preparing is 190～370nm, skewness.Envelop rate is 57%.
Comparative example 2
Take 7 grams of cephalins, 35 grams of squalenes, 5 grams of Tea Polyphenols are dissolved in 15 grams of propane diols, high speed homogenizing fully mixes it, add again 12 grams of hydroxypropyl methylcelluloses to be dissolved in the aqueous solution of 26 grams of water, stir, form stable microparticle suspending liquid, must contain the liquid precursor liposome of antioxidant squalene and tea polyphenol composition.The liquid precursor liposome particle diameter preparing is 310～580nm, skewness.Envelop rate is 41%.
Embodiment 22 application experiments
The liquid precursor liposome of above-described embodiment 1～21 is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, and every packs 1 gram into.In cigarette filter tip rod forming process, be placed in the cigarette filter of acetate fiber, built-in 1 of every cigarette filter, obtains reducing the shredded tobacco for water pipes type filter tip of cigarette gaseous phase free radical harm.Take not containing 100% liquid precursor liposome of squalene or Tea Polyphenols as contrast.
Radicals scavenging effect detection in cigarette smoke: smoke gaseous phase free radical adopts spin catching agent PBN(2-benzene tert-butylnitrone) the rear detection of trapping.
Adopt conventional gaseous phase free radical detection method: adopt glassware to collect flue gas free radical.Add the carbon tetrachloride solution of certain density free radical scavenger PBN in collector, collector is linked on smoking machine, take suction capacity as 35.0mL, suction time is 2s, and suction is spaced apart the standard aspiration speed smoking cigarette of 60s.After smoking, constant volume is collected flue gas free-atom aqueous solution, then puts into liquid nitrogen freezing.Detecting volume is 200 μ L.
The filter-tip cigarette that adds the natural synergistic composition liquid precursor liposome of different content and different proportionings by detection produces the content of free radical, compared with the control, and the removing effect of calculation composition to gaseous phase free radical in cigarette smoke.Experimental result is in Table 1:
Table 1:
Experiment conclusion: from above-described embodiment, cigarette gaseous phase free radical being removed to effect can find out, when in liquid precursor liposome containing the amount of natural during in 25～45% left and right, it freely has good removing effect to gas phase, the proportioning of particularly working as squalene and Tea Polyphenols is between 1: 8～8: 1, especially in the time of between 2: 7～7: 2, composition is removed effect to gaseous phase free radical and is had obvious synergistic effect, and its ability of removing free radical strengthens along with the increase of the amount of antioxidant.Actual according to producing, the present invention is for containing squalene and Tea Polyphenols in 30～45% left and right in the liquid precursor liposome of the natural composition of cigarette filter, its removing ability synergistic effect to gaseous phase free radical between 2: 7～7: 2 of the proportioning of squalene and Tea Polyphenols is obvious.
Claims (10)
1. containing a liquid precursor liposome for squalene and tea polyphenol composition, it is characterized in that, described liquid precursor liposome is to be prepared by each component of following mass fraction:
Hydrogenated phospholipid: 7～10 parts;
Stabilizing agent: 8～12 parts;
Surfactant: 10～15 parts;
Squalene and tea polyphenol composition: 25～45 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts;
Described hydrogenated phospholipid is one or both in hydrolecithin or hydrogenated soya phosphatide;
Described stabilizing agent is hydroxyl starch;
Described surfactant is glycerine.
2. according to claim 1 containing the liquid precursor liposome of squalene and tea polyphenol composition, it is characterized in that, is to be prepared by each component of following mass fraction:
Hydrogenated phospholipid: 8～9 parts;
Stabilizing agent: 9～11 parts;
Surfactant: 12～14 parts;
Squalene and tea polyphenol composition: 30～35 parts;
The water of surplus;
Above-mentioned each constituent mass umber sum is 100 parts.
3. according to claim 1 containing the liquid precursor liposome of squalene and tea polyphenol composition, it is characterized in that, described hydroxyl starch is one or more in hydroxymethyl starch, HES or hydroxypropul starch.
4. according to claim 1 containing the liquid precursor liposome of squalene and tea polyphenol composition, it is characterized in that, in described squalene and tea polyphenol composition, the mass ratio of squalene and Tea Polyphenols is 1: 8～8: 1.
5. described in claim 1～4 any one, contain the application of the liquid precursor liposome of squalene and tea polyphenol composition, it is characterized in that, be applied to shredded tobacco for water pipes type filter tip, be that described liquid precursor liposome is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, in cigarette filter tip rod forming process, be built in the cigarette filter of acetate fiber.
6. application according to claim 5, is characterized in that, described in built-in 0.5～1 gram of every cigarette filter containing the liquid precursor liposome of squalene and tea polyphenol composition.
7. the preparation method who contains the liquid precursor liposome of squalene and tea polyphenol composition described in claim 1～4 any one, is characterized in that, comprises the following steps:
S1. phosphatide and squalene and tea polyphenol composition are dissolved in glycerine, obtain mixed system;
S2. it is fully mixed S1 gained mixed system high speed homogenizing, obtain the mixed system of homogenizing;
S3. stabilizing agent and water are mixed to get to the aqueous solution of stabilizing agent, after adding the aqueous solution of stabilizing agent in the mixed system of S2 gained homogenizing, stir, form stable microparticle suspending liquid and obtain the liquid precursor liposome containing squalene and tea polyphenol composition.
8. according to claim 7 containing the preparation method of the liquid precursor liposome of squalene and tea polyphenol composition, it is characterized in that, the process conditions of high speed homogenizing are described in S2: 2000 r/min, stirring 45min.
9. the liquid precursor liposome containing squalene and tea polyphenol composition that described in claim 7, method prepares.
10. described in claim 9, contain the application of the liquid precursor liposome of squalene and tea polyphenol composition, it is characterized in that, it is characterized in that, be applied to shredded tobacco for water pipes type filter tip, be that described liquid precursor liposome is packed in the hollow cylinder that two ends are water proof and dust proof ventilated membrane, in cigarette filter tip rod forming process, be built in the cigarette filter of acetate fiber.
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| CN106723351A (en) * | 2016-12-28 | 2017-05-31 | 云南中烟工业有限责任公司 | A kind of grape procyanidinses nano liposomes composite membrane and its application in cigarette |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20030232419A1 (en) * | 2002-02-04 | 2003-12-18 | Kolodkin Alex L. | Molecules interacting with CASL (MICAL) polynucleotides, polypeptides, and methods of using the same |
| US20060165641A1 (en) * | 2005-01-18 | 2006-07-27 | Kumar Pillai | Cosmetic compositions containing combinations of hydroxamate derivatives and antioxidants in a liposomal delivery system |
| CN101288722A (en) * | 2007-04-19 | 2008-10-22 | 武汉三高天晨科技有限公司 | Camellia oil liposome for treating snore and xerostomia |
| CN102061174B (en) * | 2010-10-29 | 2013-04-24 | 华南农业大学 | Squalene and tea polyphenol composition and application thereof in cigarettes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20030232419A1 (en) * | 2002-02-04 | 2003-12-18 | Kolodkin Alex L. | Molecules interacting with CASL (MICAL) polynucleotides, polypeptides, and methods of using the same |
| US20060165641A1 (en) * | 2005-01-18 | 2006-07-27 | Kumar Pillai | Cosmetic compositions containing combinations of hydroxamate derivatives and antioxidants in a liposomal delivery system |
| CN101288722A (en) * | 2007-04-19 | 2008-10-22 | 武汉三高天晨科技有限公司 | Camellia oil liposome for treating snore and xerostomia |
| CN102061174B (en) * | 2010-10-29 | 2013-04-24 | 华南农业大学 | Squalene and tea polyphenol composition and application thereof in cigarettes |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106723351A (en) * | 2016-12-28 | 2017-05-31 | 云南中烟工业有限责任公司 | A kind of grape procyanidinses nano liposomes composite membrane and its application in cigarette |
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