CN103750562B - The preparation method of squalene and beta carotene composition multivesicular liposome and fall base harm reduction and apply - Google Patents
The preparation method of squalene and beta carotene composition multivesicular liposome and fall base harm reduction and apply Download PDFInfo
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Abstract
The invention discloses the preparation method of squalene and beta carotene composition multivesicular liposome and fall base harm reduction and apply.Wherein, multivesicular liposome is prepared by the phosphatide of 10 ~ 15 parts, the stabilizing agent of 10 ~ 20 parts, the surfactant of 8 ~ 15 parts and the squalene of surplus and beta carotene composition.Described multivesicular liposome is applied to cigarette can obtain good reduction free radical, reduce the effect of harm.Especially described multivesicular liposome is built in the cigarette filter of acetate fiber in cigarette filter tip rod forming process, when consumer's smoking, flue gas is by built-in filter-tip multivesicular liposome, multivesicular liposome surface area is large, ensure that antioxidant fully contacts with main flume, effectively can removing the gaseous phase free radical in main flume, providing strong technical support for solving smoking with healthy problem.
Description
Technical field
The present invention relates to technical field of chemistry, more specifically, relate to a kind of multivesicular liposome containing squalene and beta carotene composition and preparation method thereof and fall the application in base harm reduction at cigarette.
Background technology
The harm that the allow thousands of compounds of smoking generation and the radical pair human body of high concentration cause causes the most attention of people.And the effect of radical pair health endangers the most by force maximum, it can cause tuberculosis, heart disease and cancer, and these radical pairs smoker produces serious injury.Smoking produces the free radical of high concentration, comprises the phase Free Radicals that the life-span relatively grows, and quinones and half quinones, multiring aromatic hydrocarbon free radical and they and oxygen react the active oxygen radical that produces as superoxide anion (O
2 -), hydrogen peroxide (H
2o
2), hydroxy radical (OH), singlet oxygen (
1o
2) and the gaseous phase free radical relatively short with life period such as lipid peroxidation free radical (ROO), alkoxy free group (RO), alkyl diradical (R), nitroxide radical etc.
At present few in radicals scavenging research in flue gas, the injury of radical pair human body flue gas is mainly reduced from additional natural, vitamin, amino acid, protein etc. and biology enzyme free radical scavenger and trace element, natural mineral matter.But the simple mixing that these free radical scavengers generally are one or more is added in pipe tobacco.
Squalene is a kind of lipid unsaponifiable matter, for the many unsaturated olefins of one (squalene), find from the liver oil of shark at first, within 1914, be named as Squalene, its chemical name is 2, 6, 10, 15, 19, 23-hexamethyl-2, 6, 10, 14, 18, 22-tetracosa carbon six alkene, belong to open chain triterpene, also known as Squalene, participate in human body cholesterol biosynthesis and can enhance metabolism, have and improve superoxide dismutase (SOD) activity in body, strengthen body immunity, anti-ageing, antifatigue, the different physiological roles such as antitumor, it is a kind of avirulent marine biomaterial with the effect of preventing and curing diseases.Squalene distributes very wide in plant, but content is not high, and how lower than 5% of unsaponifiable matter in vegetable oil, only minority content is more, and as olive oil contains squalene 150 ~ 700mg/100g, in rice bran oil, its content is 332mg/100g.
Beta carotene is that one is extensively present in green and yellow vegetable, the natural in fruit, has the distortion of good anti-dye body, protective effect on cancer risk and stronger physiologically active.And potential aroma component important in beta carotene or tobacco, the height of its content has a great impact cigarette quality.Containing 18 Carotenoids in tobacco, carotenoid is the precursor of fragrance matter important in tobacco, in alcoholization process, can be biodegradable into more than 80 kind of aroma component through enzyme effect or air oxidation, and they have a great impact the perfume (or spice) of cigarette, taste tool.Therefore, improve the content of carotenoid in tobacco, the quality of tobacco can be improved, and the security of cigarette can be improved.
In order to overcome the shortcoming of common antioxidants, reach the object simultaneously can removing long-life phase Free Radicals and the relatively short gaseous phase free radical of life period, the applicant is devoted for years in correlative study and obtains Patents.Be in the application of ZL201010578167.8, disclose squalene and beta carotene composition and the application in cigarette thereof in the patent No., squalene and beta carotene synergistic composition directly added in pipe tobacco, obtains good effect.But further study discovery, squalene and beta carotene are easy to oxidation deterioration in atmosphere, composition is unstable when applying, and actual production is inconvenient.
Liposome (liposome) is a kind of artificial membrane.When amphiphatic molecule is scattered in aqueous phase as phosphatide and sphingolipid, the hydrophobic tail of molecule is tended to flock together, and avoids aqueous phase, and hydrophilic head is exposed to aqueous phase, formed have bilayer structure vesicle, be called liposome.According to textural classification, liposome is divided into unilamelar liposome, multilamelar liposome and multivesicular liposome.Multivesicular liposome is a kind of liposome with slow release effect, is connected and composed by many non-concentric rooms, and particle diameter, at 5 ~ 50 μm, has larger envelope volume than conventional liposome, is particularly useful for the encapsulating of water soluble drug.The application of liposome is at present mainly in medical.
These free radical scavengers are processed into multivesicular liposome, insert in cigarette filter the research of removing gaseous phase free radical (falling base) and have no report at present, more have no the technology of preparing based on the multivesicular liposome of squalene and beta carotene synergistic composition and application report.
Summary of the invention
The object of the invention is to overcome the application of existing multivesicular liposome technology in cigarette not enough, and squalene and beta carotene synergistic composition are in the technical deficiency of cigarette application aspect, provide a kind of multivesicular liposome containing squalene and beta carotene composition.
Another object of the present invention is to provide the preparation method of described multivesicular liposome.
An also goal of the invention of the present invention is to provide the described application of multivesicular liposome in cigarette containing squalene and beta carotene composition.
Object of the present invention is achieved by the following technical programs:
There is provided a kind of multivesicular liposome containing squalene and beta carotene composition, described multivesicular liposome is prepared by each component of following mass fraction:
Phosphatide: 10 ~ 15 parts;
Stabilizing agent: 10 ~ 20 parts;
Surfactant: 8 ~ 15 parts;
The squalene of surplus and beta carotene composition;
Above-mentioned each constituent mass number sum is 100 parts.
Preferably, the described multivesicular liposome containing squalene and beta carotene composition, described multivesicular liposome is prepared by each component of following mass fraction:
Phosphatide: 12 ~ 14 parts;
Stabilizing agent: 12 ~ 15 parts;
Surfactant: 10 ~ 12 parts;
The squalene of surplus and beta carotene composition;
Above-mentioned each constituent mass number sum is 100 parts.
Multi-emulsion method can be adopted to prepare the described multivesicular liposome containing squalene and beta carotene composition with above-mentioned each component.
Described phosphatide is one or more in lecithin, soybean lecithin or hydrogenated soya phosphatide.Adopt multiple phosphatide, the mixed proportion of various phosphatide does not do considered critical.
The preferred HES of described stabilizing agent, more preferably, the one of low-molecular-weight (40000 ~ 70000 dalton) HES, middle-molecular-weihydroxyethyl (130000 ~ 200000 dalton) HES and HMW (450000 ~ 480000 dalton) HES is adopted.
Described surfactant be preferably in hexadecanol, octadecyl alcolol, glycerine or shitosan (molecular weight does not do considered critical) one or more.Adopt kinds of surface activating agent, the mixed proportion of various surfactant does not do considered critical.
In described squalene and beta carotene composition, the mass ratio of squalene and beta carotene is 1: 9 ~ 9: 1, and its optimized proportion is: 1: 3 ~ 3: 1.
Described squalene can be the extract that extracts from plant, animal or microorganism or purified after sterling, can commercial products be adopted.
Described beta carotene can be the carrotene extract extracted from plant also can be purified after sterling, can commercial products be adopted.
Invention also provides the described preparation method containing the multivesicular liposome of squalene and beta carotene composition, comprise the following steps:
S1. phosphatide and squalene and beta carotene composition are dissolved in propane diols, obtain mixed system;
S2. S1 gained mixed system is transferred in the aqueous solution containing surfactant, high speed homogenizingly makes it fully mix, obtain the mixed system of homogenizing;
S3. in the mixed system of S2 gained homogenizing, add stabilizing agent to stir, form stable microparticle suspending liquid;
S4. namely the freeze drying of S3 gained microparticle suspending liquid is obtained the multivesicular liposome containing squalene and beta carotene composition.
Wherein, the consumption of propane diols described in S1 is determined according to the dissolubility of mixture;
Preferably, described in S2 containing the aqueous solution of surfactant be by surfactant and water according to: the mass ratio of 16 ~ 30:100 is mixed to get;
Preferably, process conditions high speed homogenizing described in S2 are: 2000 r/min, stir 30min;
Preferably, described in S4, cryodesiccated process conditions are :-25 DEG C of freezing 1h, freeze drying (5 × 10
-4pa, 10h).
The invention provides the multivesicular liposome containing squalene and beta carotene composition that said method prepares.
The multivesicular liposome containing squalene and beta carotene composition of the present invention obtains in cigarette well to be applied, preferably application process is: in cigarette filter tip rod forming process, multivesicular liposome of the present invention is made an addition in the cigarette filter of acetate fiber, obtain a kind of new cigarette filter, be applied to cigarette and effectively can reduce the harm of cigarette free radical, play the effect of falling base harm reduction.
Beneficial effect of the present invention:
The technology of liposome especially multivesicular liposome is applied in cigarette by the present invention first, and successfully in conjunction with squalene and beta carotene synergistic composition, sum up suitable compatibilizers and proportion compatibility, prepare the multivesicular liposome containing squalene and beta carotene synergistic composition, for the further developing application of liposome provides strong technical support simplely.
Synergistic composition is successfully embedded in multivesicular liposome by the present invention, overcome the shortcoming that squalene and beta carotene are easy to oxidation deactivation in atmosphere, by squalene and beta carotene mixture synergistic composition microencapsulation, reach the stability increasing squalene and beta carotene mixture synergistic composition, obtain the multivesicular liposome that can be applied to cigarette filter.
The present invention accurately limits material combination and preparation process condition, process the multivesicular liposome gas permeability obtained strong, surface area is large, be applicable to the application demand of cigarette filter, cigarette filter can be directly applied to, antioxidant fully can contact with main flume, reaches the object removing free radical in main flume, provides strong technical support for solving smoking with healthy problem.
Detailed description of the invention
The present invention is further described below in conjunction with specific embodiment.The test method used in following embodiment if no special instructions, is conventional method; The material used, reagent etc. if no special instructions, are the reagent that can obtain from routine business approach and material.
Embodiment 1
Each component is prepared: 15 grams, lecithin (g) according to following mass fraction; (the true Bioisystech Co., Ltd in Shanghai, content is greater than 96.0% to squalene, and following embodiment is same.But therefore do not limit the present invention, those skilled in the art can adopt other squalenes of originating) 5 grams (g); (Li Nuo bio tech ltd, Zhengzhou, content is greater than 96.0% to beta carotene, and following embodiment is same.But therefore do not limit the present invention, those skilled in the art can adopt other beta carotenes of originating) 45 grams (g); Hexadecanol 15 grams (g); Low-molecular-weight HES 20 grams (g).
Preparation process is:
S1. phosphatide and squalene and beta carotene are dissolved in 100mL propane diols, obtain mixed system;
S2. S1 gained mixed system is transferred in the 50mL water containing 15g hexadecanol, high speed homogenizingly makes it fully mix, obtain the mixed system of homogenizing;
S3. in the mixed system of S2 gained homogenizing, add low-molecular-weight HES to stir, form stable microparticle suspending liquid;
S4. namely the freeze drying of S3 gained microparticle suspending liquid is obtained the multivesicular liposome containing squalene and beta carotene composition.
Embodiment 2
Each component is prepared: soybean lecithin 15 grams according to following mass fraction; Squalene 10 grams; Beta carotene 40 grams; Octadecyl alcolol 15 grams; Low-molecular-weight HES 20 grams.
Preparation process is:
S1. soybean lecithin and squalene and beta carotene are dissolved in 100 milliliters of (mL) propane diols, obtain mixed system;
S2. S1 gained mixed system is transferred in the 50mL water containing 15g octadecyl alcolol, high speed homogenizingly makes it fully mix, obtain the mixed system of homogenizing;
S3. in the mixed system of S2 gained homogenizing, add low-molecular-weight HES to stir, form stable microparticle suspending liquid;
S4. namely the freeze drying of S3 gained microparticle suspending liquid is obtained the multivesicular liposome containing squalene and beta carotene composition.
Water and propane diols is used in the preparation process of described multivesicular liposome, but propane diols and the last all dryings removing of water, do not count the component of composition, lower same.
Embodiment 3
Each component is prepared: hydrogenated soya phosphatide 15 grams according to following mass fraction; Squalene 15 grams; Beta carotene 35 grams; Glycerine 15 grams; Medium molecular weight hydroxyethyl starch 20 grams.
Preparation process is:
S1. hydrogenated soya phosphatide and squalene and beta carotene are dissolved in 100 milliliters of (mL) propane diols, obtain mixed system;
S2. S1 gained mixed system is transferred in the 50mL water containing 15g glycerine, high speed homogenizingly makes it fully mix, obtain the mixed system of homogenizing;
S3. in the mixed system of S2 gained homogenizing, add medium molecular weight hydroxyethyl starch to stir, form stable microparticle suspending liquid;
S4. namely the freeze drying of S3 gained microparticle suspending liquid is obtained the multivesicular liposome containing squalene and beta carotene composition.
Embodiment 4
Preparation process is with embodiment 1,2 or 3.Taking 15 grams of hydrogenated soya phosphatides, 25 grams of squalenes and 25 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 15 grams of shitosans, high speed homogenizingly it is made fully to mix, add 20 grams of high molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 5
Preparation process is with embodiment 1,2 or 3.Taking 15 grams of soybean lecithins, 35 grams of squalenes and 15 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 15 grams of shitosans, high speed homogenizingly it is made fully to mix, add 20 grams of medium molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 6
Preparation process is with embodiment 1,2 or 3.Taking 15 grams of lecithin, 40 grams of squalenes and 10 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 15 grams of glycerine, high speed homogenizingly it is made fully to mix, add 20 grams of low-molecular-weight HESs again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 7
Preparation process is with embodiment 1,2 or 3.Taking 15 grams of lecithin, 45 grams of squalenes and 5 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 15 grams of octadecyl alcolol, high speed homogenizingly it is made fully to mix, add 20 grams of high molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 8
Preparation process is with embodiment 1,2 or 3.Taking 12 grams of hydrogenated soya phosphatides, 8 grams of squalenes and 54 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 10 grams of hexadecanols, high speed homogenizingly it is made fully to mix, add 16 grams of low-molecular-weight HESs again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 9
Preparation process is with embodiment 1,2 or 3.Taking 12 grams of hydrogenated soya phosphatides, 15 grams of squalenes and 47 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 10 grams of glycerine, high speed homogenizingly it is made fully to mix, add 16 grams of high molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 10
Preparation process is with embodiment 1,2 or 3.Taking 12 grams of hydrogenated soya phosphatides, 30 grams of squalenes and 32 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 10 grams of shitosans, high speed homogenizingly it is made fully to mix, add 16 grams of medium molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 11
Preparation process is with embodiment 1,2 or 3.Taking 12 grams of soybean lecithins, 40 grams of squalenes and 22 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 10 grams of shitosans, high speed homogenizingly it is made fully to mix, add 16 grams of high molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 12
Preparation process is with embodiment 1,2 or 3.Taking 12 grams of soybean lecithins, 52 grams of squalenes and 10 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 10 grams of octadecyl alcolol, high speed homogenizingly it is made fully to mix, add 16 grams of low-molecular-weight HESs again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 13
Preparation process is with embodiment 1,2 or 3.Taking 10 grams of hydrogenated soya phosphatides, 10 grams of squalenes and 62 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 8 grams of shitosans, high speed homogenizingly it is made fully to mix, add 10 grams of high molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 14
Preparation process is with embodiment 1,2 or 3.Taking 10 grams of lecithin, 20 grams of squalenes and 52 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 8 grams of hexadecanols, high speed homogenizingly it is made fully to mix, add 10 grams of medium molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 15
Preparation process is with embodiment 1,2 or 3.Taking 10 grams of lecithin, 36 grams of squalenes and 36 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 8 grams of glycerine, high speed homogenizingly it is made fully to mix, add 10 grams of high molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 16
Preparation process is with embodiment 1,2 or 3.Taking 10 grams of soybean lecithins, 50 grams of squalenes and 22 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 8 grams of shitosans, high speed homogenizingly it is made fully to mix, add 10 grams of low-molecular-weight HESs again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.
Embodiment 17
Preparation process is with embodiment 1,2 or 3.Taking 10 grams of hydrogenated soya phosphatides, 62 grams of squalenes and 10 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 8 grams of octadecyl alcolol, high speed homogenizingly it is made fully to mix, add 10 grams of medium molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtained containing squalene and beta carotene composition of freeze-drying.
The multivesicular liposome testing result prepared by above-described embodiment 1 ~ 17 is as follows: adopt laser scattering method to detect, the multivesicular liposome particle diameter that embodiment 1 ~ 17 prepares is 800 ~ 1000nm, is evenly distributed, and in normality, span is little.
Sephadex separation method is adopted to be separated with liposome with beta carotene by the squalene dissociated in solution, respectively mensuration, computational envelope rate.According to (in liposome in squalene and beta carotene composition quality/liposome squalene and beta carotene composition gross mass) × 100% computational envelope rate.The multivesicular liposome envelop rate that the embodiment of the present invention 1 ~ 17 prepares is 80 more than %.
Comparative example 1
Preparation process is with embodiment 1,2 or 3.Taking 6 grams of hydrogenated soya phosphatides, 39 grams of squalenes and 10 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 20 grams of octadecyl alcolol, high speed homogenizingly it is made fully to mix, add 25 grams of medium molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtained containing squalene and beta carotene composition of freeze-drying.The multivesicular liposome particle diameter prepared is 1500 ~ 2000nm, skewness.Envelop rate is 48%.
Comparative example 2
Preparation process is with embodiment 1,2 or 3.Taking 10 Borneo camphor phosphatide, 50 grams of squalenes and 22 grams of beta carotenes is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 8 grams of gelatin, high speed homogenizingly it is made fully to mix, add 10 grams of hydroxypropyl methylcelluloses again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.The multivesicular liposome particle diameter prepared is 1560 ~ 2100nm, skewness.Envelop rate is 35%.
Embodiment 18 application experiment
In cigarette filter tip rod forming process, multivesicular liposome described in above-described embodiment 1 ~ 17 is placed in the cigarette filter of acetate fiber, often prop up multivesicular liposome described in built-in 0.5, the 0.8 and 1 gram of embodiment 1 ~ 17 of cigarette filter, obtain the cigarette filter that can reduce cigarette gaseous phase free radical and tar harm.Not contain the blank multivesicular liposome of squalene or beta carotene, (taking 65 grams of hydrogenated soya phosphatides is dissolved in 100 milliliters of propane diols, be transferred in 50 ml waters containing 15 grams of octadecyl alcolol, high speed homogenizingly it is made fully to mix, add 20 grams of high molecular weight hydroxyethyl starch again to stir, then the dry multivesicular liposome obtaining squalene and beta carotene composition of freeze-drying.The blank multivesicular liposome particle diameter prepared is 900 ~ 1100nm) be contrast.
Radicals scavenging effect detection in cigarette smoke: smoke gaseous phase free radical adopts spin catching agent PBN(2-benzene tert-butylnitrone) the rear detection of trapping.
Adopt conventional gaseous phase free radical detection method: adopt glassware to collect flue gas free radical.Collector, in collector, is linked on smoking machine by the carbon tetrachloride solution adding certain density free radical scavenger PBN, take pumping volume as 35.0mL, and suction time is 2s, and puff interval is the standard aspiration speed smoking cigarette of 60s.After smoking, constant volume collects flue gas free-atom aqueous solution, then puts into liquid nitrogen freezing.Detecting volume is 200 μ L.Produce free-radical contents by the filter-tip cigarette detecting the natural synergistic composition multivesicular liposome adding different content and different ratio, compared with the control, calculation composition is to the elimination effect of gaseous phase free radical in cigarette smoke and tar.Experimental result is in table 1:
Table 1: (often prop up cigarette filter and add 0.5 gram of multivesicular liposome)
Table 2: (often prop up cigarette filter and add 0.8 gram of multivesicular liposome)
Table 3: (often prop up cigarette filter and add 1 gram of multivesicular liposome)
Experiment conclusion: as can be seen from above-described embodiment to cigarette gaseous phase free radical elimination effect, when in multivesicular liposome containing the amount of natural about 50 ~ 70% time, it freely has certain elimination effect to gas phase, particularly when the proportioning of squalene and beta carotene is between 1: 9 ~ 9: 1, especially time between 1: 3 ~ 3: 1, composition has obvious synergistic effect to gaseous phase free radical elimination effect, and the ability of its scavenging free radicals strengthens along with the increase of the amount of antioxidant.Actual according to production, the present invention to be used in the multivesicular liposome of the natural antioxidant composite in cigarette filter containing squalene and beta carotene about 60%, it is obvious to the Scavenging activity synergistic effect of gaseous phase free radical between 1: 3 ~ 3: 1 for the proportioning of squalene and beta carotene, and often props up in cigarette filter that to add multivesicular liposome amount be 0.8 gram of the best.
Claims (8)
1. contain a multivesicular liposome for squalene and beta carotene composition, it is characterized in that, described multivesicular liposome is prepared by each component of following mass fraction:
Phosphatide: 10 ~ 15 parts;
Stabilizing agent: 10 ~ 20 parts;
Surfactant: 8 ~ 15 parts;
The squalene of surplus and beta carotene composition;
Above-mentioned each constituent mass number sum is 100 parts;
Described phosphatide is one or more in lecithin or soybean lecithin;
Described stabilizing agent is HES;
Described surfactant is one or more in hexadecanol, octadecyl alcolol, glycerine or shitosan.
2. contain the multivesicular liposome of squalene and beta carotene composition according to claim 1, it is characterized in that, be prepared by each component of following mass fraction:
Phosphatide: 12 ~ 14 parts;
Stabilizing agent: 12 ~ 15 parts;
Surfactant: 10 ~ 12 parts;
The squalene of surplus and beta carotene composition;
Above-mentioned each constituent mass number sum is 100 parts.
3. contain the multivesicular liposome of squalene and beta carotene composition according to claim 1, it is characterized in that, described HES is the one of low-molecular-weight HES, medium molecular weight hydroxyethyl starch or high molecular weight hydroxyethyl starch.
4. according to claim 1 containing the multivesicular liposome of squalene and beta carotene composition, it is characterized in that, in described squalene and beta carotene composition, the mass ratio of squalene and beta carotene is 1: 9 ~ 9: 1.
5. contain the application of the multivesicular liposome of squalene and beta carotene composition described in any one of Claims 1 to 4, it is characterized in that, in cigarette filter tip rod forming process, described multivesicular liposome is made an addition in the cigarette filter of acetate fiber.
6. application according to claim 5, is characterized in that, often props up the multivesicular liposome containing squalene and beta carotene composition described in built-in 0.5 ~ 1 gram of cigarette filter.
7. contain the preparation method of the multivesicular liposome of squalene and beta carotene composition described in any one of Claims 1 to 4, it is characterized in that, comprise the following steps:
S1. phosphatide and squalene and beta carotene composition are dissolved in propane diols, obtain mixed system;
S2. S1 gained mixed system is transferred in the aqueous solution containing surfactant, high speed homogenizingly makes it fully mix, obtain the mixed system of homogenizing;
S3. in the mixed system of S2 gained homogenizing, add stabilizing agent to stir, form stable microparticle suspending liquid;
S4. namely the freeze drying of S3 gained microparticle suspending liquid is obtained the multivesicular liposome containing squalene and beta carotene composition.
8. contain the preparation method of the multivesicular liposome of squalene and beta carotene composition according to claim 7, it is characterized in that, described in S2 containing the aqueous solution of surfactant be by surfactant and water according to: the mass ratio of 16 ~ 30:100 is mixed to get.
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| CN1449746A (en) * | 2003-05-07 | 2003-10-22 | 蒋家驹 | Soft capsule containing trace element |
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| WO2004067678A1 (en) * | 2003-01-27 | 2004-08-12 | Mitsubishi Corporation | Antioxidative composition and composition for external use |
| EP1972324A1 (en) * | 2007-03-21 | 2008-09-24 | Cognis IP Management GmbH | Encapsulated liposomes |
| WO2009089117A1 (en) * | 2008-01-04 | 2009-07-16 | Hormel Foods Corporation | Encapsulation of oxidatively unstable compounds |
| US20110020519A1 (en) * | 2008-01-04 | 2011-01-27 | Aveka, Inc. | Encapsulation of oxidatively unstable compounds |
| CN102079979B (en) * | 2010-12-08 | 2013-04-24 | 华南农业大学 | Composition of squalene and beta-carotene and application thereof in cigarettes |
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