CN103749966B - Potato residue fermented feed and preparation method thereof - Google Patents
Potato residue fermented feed and preparation method thereof Download PDFInfo
- Publication number
- CN103749966B CN103749966B CN201410039337.3A CN201410039337A CN103749966B CN 103749966 B CN103749966 B CN 103749966B CN 201410039337 A CN201410039337 A CN 201410039337A CN 103749966 B CN103749966 B CN 103749966B
- Authority
- CN
- China
- Prior art keywords
- bacterium liquid
- fermentation
- bacillus subtilis
- lactobacillus plantarum
- aspergillus niger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 43
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 238000000855 fermentation Methods 0.000 claims abstract description 75
- 239000007788 liquid Substances 0.000 claims abstract description 71
- 230000004151 fermentation Effects 0.000 claims abstract description 66
- 230000001580 bacterial effect Effects 0.000 claims abstract description 45
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 38
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 36
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 36
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 35
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 35
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 34
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 34
- 239000000758 substrate Substances 0.000 claims abstract description 28
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 19
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 19
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 17
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 17
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 17
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 17
- 239000004202 carbamide Substances 0.000 claims abstract description 17
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 17
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 17
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 17
- 235000009566 rice Nutrition 0.000 claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 9
- 240000007594 Oryza sativa Species 0.000 claims abstract 3
- 240000008866 Ziziphus nummularia Species 0.000 claims abstract 3
- 241000894006 Bacteria Species 0.000 claims description 63
- 241000235004 Saccharomycopsis fibuligera Species 0.000 claims description 34
- 239000003153 chemical reaction reagent Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000010298 pulverizing process Methods 0.000 claims 1
- 239000000835 fiber Substances 0.000 abstract description 9
- 239000000463 material Substances 0.000 abstract description 4
- 235000019629 palatability Nutrition 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 102000010911 Enzyme Precursors Human genes 0.000 abstract 1
- 108010062466 Enzyme Precursors Proteins 0.000 abstract 1
- 241000235342 Saccharomycetes Species 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 15
- 241000209094 Oryza Species 0.000 description 14
- 241001247821 Ziziphus Species 0.000 description 14
- 238000000034 method Methods 0.000 description 13
- -1 3 grams Chemical compound 0.000 description 10
- 241000282898 Sus scrofa Species 0.000 description 10
- 239000002609 medium Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 4
- 235000015278 beef Nutrition 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 235000015099 wheat brans Nutrition 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 241000282887 Suidae Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 235000014590 basal diet Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-L 2-(carboxymethyl)-2-hydroxysuccinate Chemical compound [O-]C(=O)CC(O)(C(=O)O)CC([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-L 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 239000005418 vegetable material Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a potato residue fermented feed and a preparation method thereof, and relates to the field of animal feed prepared from plant materials. Taking potato residue 75-80%, dried jujube powder 10-15% and defatted rice bran 5-15% as raw and auxiliary materials, adding 0.75-1.5% of urea, 0.75-1.5% of ammonium sulfate, 0.5-0.7% of potassium dihydrogen phosphate and 0.4-0.6% of magnesium sulfate on the basis, mixing to obtain a fermentation substrate, preparing a combined zymogen bacterial solution by using bacterial solutions of bacillus subtilis, saccharomycete, aspergillus niger and lactobacillus plantarum, and performing aerobic fermentation and then anaerobic fermentation to obtain the product; the weight ratio of the fermentation substrate to the combined zymocyte bacterial liquid is 1: 1-1.2; the volume ratio of the four bacterial liquids is 1-3:1-3:1-3: 1-3. The invention can increase the true protein content of the fermentation substrate, reduce the crude fiber content, improve the palatability and the digestion utilization rate of the feed, comprehensively improve the nutritional value of the potato residue and have double benefits of economic and environmental benefits.
Description
Technical field
The present invention relates to from the obtained technical field of animal of vegetable material.
Background technology
China is potato planting the world's strongest nation.In China, potato is except directly eating, be mainly used to produce starch, often produce the fresh slag that 1 ton of farina can produce about 7000kg, fresh potato slag water content is up to 90%, carry miscellaneous bacteria many, not easily store, transport, but composition can be utilized, if not being used containing a small amount of protein and much starch, cellulose, pectin etc. again in dry, not only can cause the wasting of resources, also can to environment.If dry and be directly used as animal feed, cost is high and protein content is low, and crude fiber content is high, and palatability is poor.Therefore, develop safe and efficient effective potato residues earned value techniques, improve its nutritive value and security, there is economy and environment double benefit.
By biotechnology, particularly microbial fermentation technology process feedstuff, raising nutritive value, be the safe and efficient method of low-quality feed resource exploitation.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of potato residues fermented feed and preparation method thereof, the true protein content of fermentation substrate can be increased, reduce crude fibre amount, improve feed palatability and digestive utilization ratio, improve potato residues nutritive value comprehensively, there is economy and environment double benefit.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of potato residues fermented feed, with potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% is supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, with bacillus subtilis, saccharomycopsis fibuligera, the bacterium liquid of aspergillus niger and Lactobacillus plantarum is mixed into combined fermentation bacterial classification bacterium liquid, first aerobic fermentation, anaerobic fermentation is prepared from again, water content≤the 14wt% of described potato residue, the weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2, the bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
Preferably, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
The preparation method of potato residues fermented feed, comprises the following steps:
(1) with potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% for supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato residue.
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum, fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1-1.2,28-30 DEG C of aerobic fermentation 48-72 hour, then anaerobic fermentation 24-48 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
Preferably, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
Bacillus subtilis (Bacillussubtilis) 10075, aspergillus niger (Aspergillusniger) 40094, is purchased from Chinese industrial Microbiological Culture Collection administrative center; Saccharomycopsis fibuligera (Endomycopsisfibuligera) 20154, Lactobacillus plantarum (Lactobacillusplantarum) 11016, be purchased from Chinese agriculture Microbiological Culture Collection administrative center.
The preparation of bacillus subtilis used, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum bacterium liquid can adopt prior art, the invention provides following methods.
1, the preparation of Lactobacillus plantarum bacterium liquid
The Lactobacillus plantarum that 4 DEG C of inclined-planes are preserved is connected on MRS liquid tube culture medium and activates, with 220rmin at 37 DEG C
-1shaken cultivation 24h, cultivates seed as one-level; Be connected in MRS triangular flask fluid nutrient medium, with 220rmin at 37 DEG C with 1% inoculum concentration again
-1shaken cultivation 24h.MRS culture medium: peptone 10g, beef extract 10g, yeast extract 5g, hydrogen citrate 2g, glucose 20g, Tween 80 1ml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000ml, pH6.2 ~ 6.6,121 DEG C moist heat sterilization 15min.
2, the preparation of saccharomycopsis fibuligera bacterium bacterium liquid
The saccharomycopsis fibuligera bacterium that 4 DEG C of inclined-planes are preserved is connected on YEPD liquid tube culture medium and activates, with 220rmin at 30 DEG C
-1shaken cultivation 24h, cultivates seed as one-level; Be connected in YEPD triangular flask fluid nutrient medium, with 220rmin at 30 DEG C with 1% inoculum concentration again
-1shaken cultivation 24h.YEPD culture medium: dusty yeast 10g, peptone 20g, glucose 20g, distilled water 1000ml, pH6.0,115 DEG C of moist heat sterilization 20min.
3, the preparation of bacillus subtilis bacterium liquid
The bacillus subtilis that 4 DEG C of inclined-planes are preserved is connected on beef extract-peptone liquid tube culture medium and activates, with 220rmin at 32 DEG C
-1shaken cultivation cultivates 24h, cultivates seed as one-level; Be connected in beef extract-peptone triangular flask fluid nutrient medium, with 220rmin at 32 DEG C with 1% inoculum concentration again
-1shaken cultivation 24h.Beef-protein medium: beef-protein medium: beef extract 0.5g, peptone 15g, sodium chloride 5g, glucose 20g, distilled water 1000ml, pH7.0 ~ 7.2,115 DEG C moist heat sterilization 20min.
4, the preparation of black-koji mould liquid
The aspergillus niger that 4 DEG C of inclined-planes are preserved is connected on Cha Shi liquid tube culture medium and activates, with 220rmin at 28 DEG C
-1shaken cultivation cultivates 36h, cultivates seed as one-level; Be connected in Cha Shi triangular flask fluid nutrient medium, with 220rmin at 28 DEG C with 1% inoculum concentration again
-1shaken cultivation 36h.Czapek's medium: sodium nitrate 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, distilled water 1000ml, pH nature, 121 DEG C of moist heat sterilization 20min.
It is raw material that the present invention mainly applies potato residue, dry jujube powder and defatted rice bran are auxiliary material, add reagent on this basis: urea, ammonium sulfate, potassium dihydrogen phosphate and magnesium sulfate, be mixed to get fermentation substrate, through the combined fermentation bacterial classification bacterium liquid that the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is mixed into, first aerobic fermentation, then anaerobic fermentation is prepared from.Its principle is: potato residue, defatted rice bran and dry jujube powder contain enough starch and crude fibre and a small amount of true albumen; Urea, ammonium sulfate contain the available a large amount of inorganic nitrogen of microorganism; Magnesium sulfate, potassium dihydrogen phosphate can promote that some beneficial microbe grows; Above-mentioned three class material action compensatings, application simultaneously can produce Overlay.
Application saccharomycopsis fibuligera can improve fermentation substrate true protein content; Bacillus subtilis can produce protease, improves protein utilization rate; Aspergillus niger can eccrine fiber element enzyme degradation of fibers, also can secrete pectase thus decompose pectin; Lactobacillus plantarum can produce lactic acid, improves feed local flavor; Fermentation substrate is after aerobic, anaerobic fermentation process, and true protein content is 13.24%, and total amount improves 36.93%, and crude fiber content is 24.13%, and total amount reduces 41.00%, and has sweet-smelling smell.
The beneficial effect adopting technique scheme to produce is:
(1) the present invention can increase the true protein content of fermentation substrate, reduces crude fibre amount, improves feed palatability and digestive utilization ratio, improves potato residues nutritive value comprehensively, have economy and environment double benefit; Application the inventive method is by after fermentation substrate process, and true protein content is 13.24%, and total amount improves 36.93%, and crude fiber content is 24.13%, and total amount reduces 41.00%, and has sweet-smelling smell.
(2) product of the present invention substitutes wheat bran by 15% part by weight, can improve growing and fattening pig feed intake, daily gain, reduces feedstuff-meat ratio.
(3) the present invention is pure green product, without any side effects, does not produce incompatibility with other raw materials.
Detailed description of the invention
Potato residue is purchased from Kangbao County, Zhangjiakou moral along starch factory, and water content≤14wt%, market is on sale.
Embodiment 1
Adopt supplementary material and the reagent of following weight composition: potato residue 160 grams, 20 grams, dry jujube powder, defatted rice bran 20 grams, 3 grams, urea, 3 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: after above-mentioned supplementary material and reagent are weighed, input pulverizer is pulverized, drop into mixer again to mix, make fermentation substrate, then with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum for combined fermentation bacterial classification bacterium liquid, wherein the bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum, fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1,28-30 DEG C of aerobic fermentation 48 hours, then anaerobic fermentation 24 hours, dry to water content≤14wt% for 70-80 DEG C, pulverize.
Embodiment 2
Adopt supplementary material and the reagent of following weight composition: potato residue 160 grams, 20 grams, dry jujube powder, defatted rice bran 20 grams, 3 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1:2:2:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.2.
Embodiment 3
Adopt supplementary material and the reagent of following weight composition: potato residue 150 grams, 30 grams, dry jujube powder, defatted rice bran 20 grams, 3 grams, urea, 3 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1:1:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.1.
Embodiment 4
Adopt supplementary material and the reagent of following weight composition: potato residue 150 grams, 30 grams, dry jujube powder, defatted rice bran 20 grams, 3 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:1:2:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.05.
Embodiment 5
Adopt supplementary material and the reagent of following weight composition: potato residue 160 grams, 20 grams, dry jujube powder, defatted rice bran 20 grams, 1.5 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1:3:3:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.15.
Embodiment 6
Adopt supplementary material and the reagent of following weight composition: potato residue 150 grams, 30 grams, dry jujube powder, defatted rice bran 20 grams, 1.5 grams, urea, 1.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.2 grams, 1 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:1:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1;
Embodiment 7
Adopt supplementary material and the reagent of following weight composition: potato residue 160 grams, 30 grams, dry jujube powder, defatted rice bran 10 grams, 2 grams, urea, 1.8 grams, ammonium sulfate, potassium dihydrogen phosphate 1 gram, 0.8 gram, magnesium sulfate.
Preparation method: be to be with the difference of embodiment 1 that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:2:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1.
Embodiment 8
Adopt supplementary material and the reagent of following weight composition: potato residue 150 grams, 20 grams, dry jujube powder, defatted rice bran 30 grams, 2.5 grams, urea, 2 grams, ammonium sulfate, potassium dihydrogen phosphate 1.1 grams, 1.2 grams, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 2:3:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.2.
Embodiment 9
Adopt supplementary material and the reagent of following weight composition: potato residue 156 grams, 24 grams, dry jujube powder, defatted rice bran 20 grams, 1.7 grams, urea, 2.5 grams, ammonium sulfate, potassium dihydrogen phosphate 1.3 grams, 0.9 gram, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:3:2:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.1,28-30 DEG C of aerobic fermentation 60 hours, then anaerobic fermentation 48 hours.
Embodiment 10
Adopt supplementary material and the reagent of following weight composition: potato residue 152 grams, 28 grams, dry jujube powder, defatted rice bran 20 grams, 2.2 grams, urea, 2.7 grams, ammonium sulfate, potassium dihydrogen phosphate 1.4 grams, 1.1 grams, magnesium sulfate.
Preparation method: be that combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:2:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum with the difference of embodiment 1; Fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1.15,28-30 DEG C of aerobic fermentation 72 hours, then anaerobic fermentation 36 hours.
Its using method of potato residues fermented feed of the present invention is: substitute the wheat bran of 15% at growing-finishing pigs diets moderate with this product, other raw materials are constant.
Experimental example:
This potato residues fermented feed affects experimental design to growing-finishing pig production performance
1.1 experimental animals and grouping
Select age in days to differ and be no more than 7 days, about 30 kilograms pigs 120 that kind is identical, identical according to genetic background, that body weight is close, sex ratio is consistent principle is divided into 2 process at random, often 6 repetitions are established in process, and often repeat 10, between each group, starting weight difference is not remarkable.
Control group: basal diet of feeding; Test group: feed and substitute the test daily ration of equivalent wheat bran with 15% potato residues fermented feed.
1.2 test daily ration and Treatment Design
With reference to NRC(1998) growing-finishing pig nutrition requirements in conjunction with production practices preparation basal diet.
Table 1 Diet Formula and trophic level
1.3 feeding and management
Test is carried out in same confined swine housing, raises on the net, and humiture is suitable for; Free choice feeding and drinking-water.Immunity disinfectant program is undertaken by pig farm conventional method.Test is responsible for by special messenger, manages in strict accordance with test requirements document.Feeding and management is carried out with reference to the feeding and management code of this product boar.Test period 40 days.
1.4. test method
On-test and at the end of in units of individuality, empty stomach is carried out to all test pig and weighs, record body weight in detail; In units of repeating, feed intake is recorded in detail in process of the test; Detailed record pig death and superseded situation, cut in time when there is dead naughty pig and expect and weigh.
1.5 Testing index
The average daily gain of growth performance index, average daily ingestion amount and feedstuff-meat ratio.
1.6 data processings and statistical analysis technique
ANOVA in data acquisition SPSS13.0 statistical software carries out variance analysis; Pat everywhere and carry out the significance of difference by Duncan method between mean and compare, result of the test data represent with mean+SD.
2. results and analysis
Table 2 is on the impact of growing-finishing pig production performance
Note: not significantly (P>0.05), the different person of colleague's shoulder mark represents significant difference (P<0.05) to the identical person's difference of colleague's shoulder mark lowercase.
As can be seen from Table 2, test group off-test weight ratio control group improves 3.42%(P<0.05); Average daily gain improves 7.12%(P<0.05 than control group); Average daily ingestion amount improves 3.11%(P>0.05 than control group); Feedstuff-meat ratio reduces 3.82%(P>0.05 than control group).
3. conclusion
Can be obtained to draw a conclusion by result of the test:
With fermenting potato slag equivalent substitution wheat bran in growing and fattening pig feed, growing and fattening daily gain in pigs can be significantly improved, also trend is improved to feed intake, feedstuff-meat ratio;
Because this product is safe green feedstuff, and cost is significantly lower than substitute-wheat bran, and therefore produce market has a extensive future.
Claims (4)
1. a potato residues fermented feed, it is characterized in that: with potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% is supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, with bacillus subtilis, saccharomycopsis fibuligera, the bacterium liquid of aspergillus niger and Lactobacillus plantarum is mixed into combined fermentation bacterial classification bacterium liquid, first 28-30 DEG C of aerobic fermentation 48-72 hour, anaerobic fermentation 24-48 hour again, oven dry pulverizing is prepared from, water content≤the 14wt% of described potato residue, the weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2, the bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
2. potato residues fermented feed according to claim 1, is characterized in that described combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
3. a preparation method for potato residues fermented feed as claimed in claim 1, is characterized in that, comprise the following steps:
(1) with potato residue 75-80wt%, dry jujube powder 10-15wt% and defatted rice bran 5-15wt% for supplementary material, this supplementary material basis adds reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato residue;
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum, fermentation substrate and combined fermentation bacterial classification bacterium liquid press the weight ratio Homogeneous phase mixing of 1:1-1.2,28-30 DEG C of aerobic fermentation 48-72 hour, then anaerobic fermentation 24-48 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum is 1 × 10
6individual/ml, combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
4. the preparation method of potato residues fermented feed according to claim 3, is characterized in that described combined fermentation bacterial classification bacterium liquid is mixed according to the ratio that volume ratio is 3:3:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, aspergillus niger and Lactobacillus plantarum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410039337.3A CN103749966B (en) | 2014-01-27 | 2014-01-27 | Potato residue fermented feed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410039337.3A CN103749966B (en) | 2014-01-27 | 2014-01-27 | Potato residue fermented feed and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103749966A CN103749966A (en) | 2014-04-30 |
| CN103749966B true CN103749966B (en) | 2016-02-03 |
Family
ID=50517421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410039337.3A Active CN103749966B (en) | 2014-01-27 | 2014-01-27 | Potato residue fermented feed and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103749966B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104171408B (en) * | 2014-08-02 | 2016-12-28 | 青岛申达众创技术服务有限公司 | Utilize the method that potato residues makes feed addictive of layer chicken |
| CN104263783A (en) * | 2014-10-09 | 2015-01-07 | 哈尔滨艾克尔食品科技有限公司 | Method for preparing protein peptide through waste water produced from potato starch producing |
| CN104757331A (en) * | 2015-04-17 | 2015-07-08 | 赖世权 | Preparation method of cattle feed |
| CN104996841A (en) * | 2015-08-20 | 2015-10-28 | 兴安县森澳养殖有限公司 | Pig starter feed |
| CN105961828A (en) * | 2016-03-22 | 2016-09-28 | 安徽旺旺禽业科技有限公司 | Ganoderma lucidum feed for broiler chicken and preparation method thereof |
| CN107494927A (en) * | 2017-08-02 | 2017-12-22 | 陆丽英 | The method that sugarcane recycles |
| CN109221755A (en) * | 2018-11-12 | 2019-01-18 | 安徽科技学院 | A kind of meat rabbit fermented feed |
| CN110269145B (en) * | 2019-08-01 | 2023-06-09 | 哈尔滨工业大学 | Process method for producing cellular protein by fermenting potato starch industrial byproducts |
| CN112715780A (en) * | 2020-12-23 | 2021-04-30 | 武汉轻工大学 | Composite zymophyte, and preparation method and application of broad bean meal fermented product |
| CN114304377A (en) * | 2021-12-27 | 2022-04-12 | 乌兰察布职业学院 | Preparation method of potato residue feed |
| CN114766596A (en) * | 2022-05-09 | 2022-07-22 | 广东大泽农生物科技股份有限公司 | Degradation method of coix seed bran and Chinese gall residue, application of degradation method and biological fermentation feed |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899079A (en) * | 2005-07-19 | 2007-01-24 | 中国农业机械化科学研究院 | Method for preparing protein feed by solid fermenting potato slag |
| CN101041807A (en) * | 2007-03-06 | 2007-09-26 | 北京万泰新源生物科技有限公司 | Fertilizer leaven and preparation method and application thereof |
| CN102125169A (en) * | 2011-01-06 | 2011-07-20 | 宜春强微生物科技有限公司 | Dreg feed leaven and application thereof |
-
2014
- 2014-01-27 CN CN201410039337.3A patent/CN103749966B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899079A (en) * | 2005-07-19 | 2007-01-24 | 中国农业机械化科学研究院 | Method for preparing protein feed by solid fermenting potato slag |
| CN101041807A (en) * | 2007-03-06 | 2007-09-26 | 北京万泰新源生物科技有限公司 | Fertilizer leaven and preparation method and application thereof |
| CN102125169A (en) * | 2011-01-06 | 2011-07-20 | 宜春强微生物科技有限公司 | Dreg feed leaven and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 利用马铃薯淀粉废渣厌氧发酵生产饲料的技术;王志民等;《四川农业科技》;20101231(第08期);42-43 * |
| 复合微生物发酵马铃薯渣生产蛋白饲料的工艺研究;沈讷敏等;《广东农业科学》;20111231(第18期);66-68 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103749966A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103749966B (en) | Potato residue fermented feed and preparation method thereof | |
| CN103749967B (en) | Sweet potato dregs fermented feed and preparation method thereof | |
| CN102845592B (en) | Method for producing bio-protein feedstuff through solid-state fermentation of dreg silybum marianum dreg | |
| CN108157673A (en) | A kind of preparation method for the prawn mixed feed that ferments | |
| CN102823726A (en) | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity | |
| CN101429059A (en) | Green organic agronomic crop microorganism bacterial manure and method of producing the same | |
| CN106879821A (en) | The method that Jujun grasses fermentation prepares cattle and sheep and Feeds of vegetarian | |
| CN105918614A (en) | Integration processing method of high-efficient corn straw biological feed | |
| CN104757267A (en) | Apple pomace microbial culture starter and method for producing biological feed by apple pomace microbial culture starter | |
| CN103283955A (en) | Method for preparing biological feed from rice straw | |
| CN106173365A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Flos Jasmini Sambac slag | |
| CN100391357C (en) | Bacilli fungi mixed fermentation for producing microbial formulation and compound enzyme feed additive | |
| CN100408673C (en) | Organic microbial composite and use | |
| CN106173364A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with corn straw | |
| CN106234755B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using bagasse | |
| CN106212916A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with sugarcane tail | |
| CN106173204A (en) | A kind of method preparing high protein feed for base material fermentation with citric acid corn starch residue and mycelia slag | |
| CN1318582C (en) | Once aspergillus niger fermentation process of obtaining complex enzyme of several kinds of enzyme and with high enzyme activity | |
| CN101407762B (en) | Microbial solid inocula, and preparation and use thereof | |
| CN104472882A (en) | Liquid biological feed additive | |
| CN104605138A (en) | Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof | |
| CN1934989A (en) | Microbial fodder additive | |
| CN105533134A (en) | Preparation method of fermentation feed | |
| CN103960463B (en) | Ensiling tangerine dregs of rice feed and preparation technology thereof | |
| CN102224885B (en) | Production method of liquid phagostimulant for fattening pigs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180711 Address after: 100081, 16 floor, block B, 1 building, 38 south street avenue, Haidian District, Beijing, 2014, Zhongguancun. Patentee after: BOYIDE (BEIJING) BIOTECHNOLOGY Co.,Ltd. Address before: No. 2598, Le Kennan street, Baoding, Hebei Patentee before: HEIBEI AGRICULTURAL UNIVERSITY |