CN103705696A - Method for preparing powder injection for treating diabetes by using aloes and Chinese yam - Google Patents
Method for preparing powder injection for treating diabetes by using aloes and Chinese yam Download PDFInfo
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- CN103705696A CN103705696A CN201310731324.8A CN201310731324A CN103705696A CN 103705696 A CN103705696 A CN 103705696A CN 201310731324 A CN201310731324 A CN 201310731324A CN 103705696 A CN103705696 A CN 103705696A
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Abstract
The invention discloses a method for preparing powder injection for treating diabetes by using aloes and Chinese yam. The method comprises the following steps: uniformly mixing aloe powder and Chinese yam powder according to a ratio, adding distilled water to obtain a 5% aloe and Chinese yam mixed aqueous solution so that the powder is completely dissolved, standing over the night and separating, and filtering and clarifying the supernatant; regulating the pH value to be 6.3-6.5 by using hydrochloric acid, heating, sterilizing and naturally cooling; regulating the pH value of the cooling liquid to be 4.2-4.5 by using hydrochloric acid, performing ultrafiltration through an ultrafiltration membrane for retaining a drug-containing solution of which the molecular weight is not more than 10000Da, subpackaging the solution in an ampoule bottle, and carrying out freeze drying to obtain the finished powder injection. The method has the advantages that a traditional application range of taking the aloes and Chinese yam as common foods is broken, the modern medicine means is adopted, and the aloes and Chinese yam are compatible to be prepared into the powder injection, so that the medicinal field of the powder injection is widened to treatment of diabetes. The test proves that the powder injection for treating diabetes is outstanding in curative effect and does not have a toxic or side effect.
Description
Technical field
The present invention relates to treat the injection of diabetes, especially relate to a kind of method of using Aloe, Rhizoma Dioscoreae to prepare treatment diabetes injection liquid injectable powder.
Background technology
Diabetes are a kind of metabolic diseases that hyperglycemia is feature of take, be due to defect of insulin secretion or its biological agent impaired, or both have concurrently and cause, the long-standing hyperglycemia of diabetes can cause each tissue of health, particularly eye, kidney, heart, blood vessel, neural chronic lesion, dysfunction.Medicine for treating diabetes comprises sulfonylurea drugs, biguanides, α glucosidase inhibitor, euglycemic agent etc. at present, or employing insulinize, though these medicines are evident in efficacy to some symptom, but side effect is larger, long-term taking can bring other harmful effect to health.
Aloe (Aloe) is a kind of herbaceous plant of perennial Liliaceae meat, is a kind of plant that has edible, beauty treatment, health care, medicine and the function such as view and admire.Aloe complex chemical composition, the composition of having found surpasses kind more than 160, mainly contains the effective active compositions such as polysaccharide, anthraquinone analog compound, organic acid, aminoacid, enzyme and polypeptide, vitamin, steroid, mineral, trace element.Aloe industry mainly contains the basic materials such as Aloe (Aloe fourth) canned food, aloe liquid, aloe leaf powder, aloe gel powder at present, is used for manufacturing the oral aloe products such as Aloe glue, aloe capsule, Aloe tablet, Aloe extract.Oral aloe products has eliminating toxin and beautifying the skin, and the logical the intestines and stomach of profit, strengthens the effects such as immunologic function, but actual effect mainly concentrates on bowel relaxing functions, and other human body diseases are not had clear improvement and therapeutical effect.The long-term development of my company's process, uses modern medicine and pharmacology means, and Aloe has been made to injection lyophilized powder, and its medicinal field has been widened the control of the diseases such as tumor, hepatic injury and treatment aspect.But so far, not yet there is people aloe product to be applied to the medicine aspect of preparation treatment diabetes.
Rhizoma Dioscoreae general designation Rhizoma Dioscoreae, herbaceos perennial, stem is overgrow, and tuber is cylindrical, starch-containing and protein, sweet in the mouth is flat, returns spleen, lung, kidney channel, always for temperature compensation, nourishes, and adjusts hormonal system.Because Rhizoma Dioscoreae contains mucus protein, there is the hypoglycemic effect of falling, be also diabetes patient's dietetic therapy good merchantable brand.At present Rhizoma Dioscoreae is except edible as daily bread steaming and decocting, and deep processing project is only for Rhizoma Dioscoreae powder, yam drink, decoction pieces, and starch etc., further deep processing have not been reported.
Summary of the invention
The object of the present invention is to provide a kind of method of using Aloe, Rhizoma Dioscoreae to prepare treatment diabetes injection liquid injectable powder.
For achieving the above object, the present invention can take following technical proposals:
The method that use Aloe of the present invention, Rhizoma Dioscoreae are prepared treatment diabetes injection liquid injectable powder comprises the steps:
The first step, by Aloe powder and Rhizoma Dioscoreae powder according to the weight portion proportioning mix homogeneously of 50-99:1-50; Then in the ratio of adding 2000 weight portion distilled water in 100 weight portion mixed powders, obtain 5% mixed aqueous solution;
Second step, stirs the mixed aqueous solution of the first step, and Aloe powder and Rhizoma Dioscoreae powder are dissolved completely, and standing over night is separated;
The 3rd step, by the supernatant liquid filtering clarification after second step separation;
The 4th step, the filtrate that the 3rd step is obtained is adjusted to 6.3-6.5 with hydrochloric acid by pH value, is heated to 110 ℃ of-120 ℃ of sterilizings 30 minutes, then natural cooling;
The 5th step, the pH value of the liquid coolant the 4th step being obtained with hydrochloric acid is adjusted to 4.2-4.5, through ultrafilter membrane ultrafiltration, holds back and obtains the drug solns that contains that molecular weight is not more than 10000Da;
The 6th step, the medicinal liquid that the 5th step is obtained is divided in lyophilizing in ampoule bottle can obtain injection injectable powder finished product.
For guaranteeing the quality of injection, the preparation method of Aloe powder used is:
The first step, soaks 30-60 minute by fresh aloe leaf, is ground into Aloe juice after cleaning up;
Second step, pours Aloe juice in reacting by heating tank into and imports and be steam heated to 8-12 ℃, adds pectase or protease by 0.125% of Aloe juice gross weight simultaneously, after 50-60 minute, temperature is risen to 75-80 ℃, and inactivation treatment is carried out in insulation for 3-5 hour;
The 3rd step, by second step after inactivation treatment the dry or lyophilization of solution filter, concentrated final vacuum to obtain outward appearance be flaxen Aloe powder finished product.
The preparation method of Rhizoma Dioscoreae powder used is:
The first step, soaks 30-60 minute by Rhizoma Dioscoreae root, cleans up the superfine powder that post-drying is ground into 0.02-15 micron;
Second step, the Rhizoma Dioscoreae superfine powder that the first step is obtained is poured in reacting by heating tank after adding distilled water to dissolve in the ratio of 100:2000 weight portion, imports and is steam heated to 75-80 ℃, insulation 3-5 hour, it is flaxen Rhizoma Dioscoreae powder finished product that the dry or lyophilization of concentrated final vacuum obtains outward appearance.
During actual preparation, the weight portion proportioning of Aloe powder and Rhizoma Dioscoreae powder can be 50-80:50-20.
When the weight portion proportioning of Aloe powder and Rhizoma Dioscoreae powder is 50-60:50-40, curative effect can be better.
The invention has the advantages that broken through traditional by Aloe, Rhizoma Dioscoreae the scope of application as bread and cheese, use modern medicine and pharmacology means, Aloe and Rhizoma Dioscoreae compatibility are prepared into injection lyophilized powder, make its medicinal field widen treating diabetes aspect.Evidence, the injection injectable powder curative effect for the treatment of diabetes prepared by the present invention is given prominence to and is had no side effect.
The specific embodiment
The method that use Aloe of the present invention, Rhizoma Dioscoreae are prepared treatment diabetes injection liquid injectable powder is as follows:
One, prepare Aloe powder:
The first step, soaks 30-60 minute by fresh aloe leaf, cleans up rear employing food and is ground into Aloe juice with rustless steel disintegrating machine, Folium Aloe peeling can be pulverized, or do not remove the peel full leaf and pulverize during pulverizing;
Second step, pours Aloe juice in reacting by heating tank into and imports and be steam heated to 8-12 ℃, adds pectase or protease by 0.125% of Aloe juice gross weight simultaneously, after 50-60 minute, temperature is risen to 75-80 ℃, and inactivation treatment is carried out in insulation for 3-5 hour;
The 3rd step, by second step after inactivation treatment solution adopt shake sieve, kieselguhr or plate-and-frame filtration, also two kinds of modes can be combined and filter, if need decolouring, also can add the active carbon processing of decolouring; Concentrated rear (soluble solid of concentrated juice is controlled in 10%) adopts spraying method dry or lyophilizing to be made into outward appearance is flaxen Aloe powder finished product.
Two, prepare Rhizoma Dioscoreae powder:
The first step, soaks 30-60 minute by Rhizoma Dioscoreae root, cleans up the superfine powder that post-drying is ground into 0.02-15 micron;
Second step, the Rhizoma Dioscoreae superfine powder that the first step is obtained is poured in reacting by heating tank after adding distilled water to dissolve in the ratio of 100:2000 weight portion, imports and is steam heated to 75-80 ℃, insulation 3-5 hour, it is flaxen Rhizoma Dioscoreae powder finished product that the dry or lyophilization of concentrated final vacuum obtains outward appearance.
Three, prepare injection injectable powder:
The first step, the Rhizoma Dioscoreae powder mix homogeneously of the Aloe powder of 50-99 weight portion and 1-50 weight portion (during actual preparation, the larger curative effect of addition of Rhizoma Dioscoreae powder is better: when the weight portion proportioning of Aloe powder and Rhizoma Dioscoreae powder is 50-60:50-40, curative effect is best) is obtained to basic powder; Get basic powder 100g and 2000ml distilled water and prepare the mixed aqueous solution (can amplify in this ratio during commercial production) that obtains 5%;
Second step, stirs mixed aqueous solution or ultrasonic dissolution 15 minutes,, Aloe powder and Rhizoma Dioscoreae powder are dissolved completely and obtain light brown solution, standing over night separated (can use the magnificent high pressure homogenize machine equipment in east, Shanghai);
The 3rd step, by the supernatant liquid filtering clarification after second step separation;
The 4th step, the filtrate that the 3rd step is obtained is adjusted to 6.3-6.5 with 37% hydrochloric acid by pH value, is heated to 110 ℃ of-120 ℃ of high temperature sterilizes 30 minutes, then natural cooling;
The 5th step, the pH value of the liquid coolant that the hydrochloric acid with 37% obtains the 4th step is adjusted to 4.2-4.5, use ultrafiltration technology, through ultrafilter membrane ultrafiltration, hold back obtain molecular weight be not more than 10000Da containing drug solns (the full-automatic membrance separation ultrafiltration apparatus that can use Hefei Fengyun Membrane Technology Co., Ltd. to produce during commercial production, laboratory is used No. 4 pans);
The 6th step, the medicinal liquid that the 5th step is obtained is divided in 10ml ampoule bottle, the injection injectable powder finished product that-30 ℃ of lyophilizing (the JDG-100F fully automatic vacuum freeze dryer that can use Lanzhou Kejin Vacuum Freeze Drying Technology Co., Ltd. to produce) diabetes that can obtain medical treatment are used.
During use, this injectable powder is dissolved into injection with 0.9% normal saline dilution.
The clinical result of use experiment of injection injectable powder of the present invention is as follows:
1, experiment purpose: observe blood sugar reducing function and the impact on insulin of injection injectable powder of the present invention.
2, experiment material:
2.1 animals: Healthy female SD rat, body weight 200-220g, is provided by Yangzhou University's comparative medicine center, production licence: SCXK (Soviet Union) 2012-0004.
2.2 medicines and reagent:
The injection injectable powder that Aloe powder and Rhizoma Dioscoreae powder make by 60:40 weight portion, is brown, specification: every bottle containing the about 1g of soluble component.During administration, directly use Injectable sterile physiological saline solution, and be diluted to the sample (referred to as J-11) of two kinds of concentration (120 mg/kg, 240 mg/kg), the administration volume of two kinds of samples is 0.1ml/10g, is intraperitoneal injection.
Streptozotocin (STZ), 572201, MERCK subpackage.
Glucose kit, lot number: 20130822, by Shanghai Kehua Bio-engineering Co., Ltd.
Glyburide, lot number: 20130527, Tianjin Pacific Pharmaceutical Co., Ltd..
Instrument: 7020 full-automatic Hitachi's biochemical instruments (HITACHI).
3, experimental technique:
3.1 reagent preparations:
STZ is dissolved in the citrate buffer solution of 0.1M, and pH4.5 left and right is now with the current, 0.2ml/100g body weight.
Being formulated as follows of 0.1mol/L citrate buffer solution:
0.1mol/L citric acid mother liquor: claim 2.10g citric acid to be dissolved in 100ml distilled water.
0.1mol/L trisodium citrate mother solution: claim 2.94g trisodium citrate to be dissolved in 100ml distilled water.
0.1mol/L citrate buffer solution (pH4.5) is to be formed by 0.1mol/l citric acid mother liquor and 0.1mol/l trisodium citrate mother solution mixed preparing, and ratio is 1:1.32.
Reagent dosage: 50 ~ 60mg/Kg lumbar injection, generally four days to can modeling once week successfully, blood glucose is greater than 11mmol/L and thinks diabetes model animal.
3.2 modeling methods:
By 50 mg/kg, to rat disposable celiac, inject 0.5% streptozotocin solution, after 72h, from rat tail vein, get blood, centrifugalize serum, full-automatic Hitachi biochemical instruments is measured fasting glucose.Blood glucose value >=11mmol/ L person is defined as to diabetes model rat.
3.3 grouping and dosage:
60 diabetes model rats are divided into five groups: J-11 high dose group (120 mg/kg), J-11 low dose group (240 mg/kg), glyburide group (8mg/kg), model group and blank group, 10 every group.4 groups of intraperitoneal injections wherein, continuous 7 days.Detect index: blood glucose value and insulin.Detection time: before administration, after administration 7 days.
4, statistical analysis: between each group, adopt the comparison of T method of inspection, with p<0.05 or p<0.01 for thering is significant difference.
5, experimental result sees the following form
The blood sugar reducing function (M ± SD) of J-11 to the high sugared rat model of STZ
Note: with matched group comparison, ## p<0.01; With model group comparison, * p<0.05, * * p<0.01.
The influence (M ± SD) of J-11 to the high sugared rat model insulin of STZ
Note: with matched group comparison, ## p<0.01; With model group comparison, * p<0.05, * * p<0.01.
Above data result demonstration, after rats by intraperitoneal injection STZ, significantly raising appears in blood glucose, and average reaches 16.44 mmol/L, and hyperglycemia is more stable, and the persistent period is longer.After animal subject administration 7 days, in administration, within seven days, there is afterwards decline by a relatively large margin in the high and low dose group rat blood sugar value of J-11 sample, mean blood glucose is respectively 11.15 mmol/L and 11.74 mmol/L, through check and model group, relatively presents significant difference (P < 0.05).Insulin aspect, after J-11 effect hyperglycemia rat, the serum insulin value of its high and low dose rat is respectively 14.31 μ U/ml and 14.46 μ U/ml, with the 11.33 μ U/ml comparisons of model group rat insulin average, manifest obvious diversity, illustrate that J-11 has the effect that promotes insulin secretion.
6, conclusion: above-mentioned experimental result shows, treatment diabetes injection liquid injectable powder prepared by the present invention has obvious blood sugar reducing function, and its blood sugar reducing function and J-11 promote that insulin secretion is closely related.
Claims (5)
1. use Aloe, Rhizoma Dioscoreae to prepare a method for treatment diabetes injection liquid injectable powder, it is characterized in that: comprise the steps:
The first step, by Aloe powder and Rhizoma Dioscoreae powder according to the weight portion proportioning mix homogeneously of 50-99:1-50; Then in the ratio of adding 2000 weight portion distilled water in 100 weight portion mixed powders, obtain 5% mixed aqueous solution;
Second step, stirs the mixed aqueous solution of the first step, and Aloe powder and Rhizoma Dioscoreae powder are dissolved completely, and standing over night is separated;
The 3rd step, by the supernatant liquid filtering clarification after second step separation;
The 4th step, the filtrate that the 3rd step is obtained is adjusted to 6.3-6.5 with hydrochloric acid by pH value, is heated to 110 ℃ of-120 ℃ of sterilizings 30 minutes, then natural cooling;
The 5th step, the pH value of the liquid coolant the 4th step being obtained with hydrochloric acid is adjusted to 4.2-4.5, through ultrafilter membrane ultrafiltration, holds back and obtains the drug solns that contains that molecular weight is not more than 10000Da;
The 6th step, the medicinal liquid that the 5th step is obtained is divided in lyophilizing in ampoule bottle can obtain injection injectable powder finished product.
2. use Aloe according to claim 1, Rhizoma Dioscoreae are prepared the method for the treatment of diabetes injection liquid injectable powder, it is characterized in that: the preparation method of described Aloe powder is:
The first step, soaks 30-60 minute by fresh aloe leaf, is ground into Aloe juice after cleaning up;
Second step, pours Aloe juice in reacting by heating tank into and imports and be steam heated to 8-12 ℃, adds pectase or protease by 0.125% of Aloe juice gross weight simultaneously, after 50-60 minute, temperature is risen to 75-80 ℃, and inactivation treatment is carried out in insulation for 3-5 hour;
The 3rd step, by second step after inactivation treatment the dry or lyophilization of solution filter, concentrated final vacuum to obtain outward appearance be flaxen Aloe powder finished product.
3. use Aloe according to claim 1, Rhizoma Dioscoreae are prepared the method for the treatment of diabetes injection liquid injectable powder, it is characterized in that: the preparation method of described Rhizoma Dioscoreae powder is:
The first step, soaks 30-60 minute by Rhizoma Dioscoreae root, cleans up the superfine powder that post-drying is ground into 0.02-15 micron;
Second step, the Rhizoma Dioscoreae superfine powder that the first step is obtained is poured in reacting by heating tank after adding distilled water to dissolve in the ratio of 100:2000 weight portion, imports and is steam heated to 75-80 ℃, insulation 3-5 hour, it is flaxen Rhizoma Dioscoreae powder finished product that the dry or lyophilization of concentrated final vacuum obtains outward appearance.
4. use Aloe according to claim 1, Rhizoma Dioscoreae are prepared the method for the treatment of diabetes injection liquid injectable powder, it is characterized in that: the weight portion proportioning of described Aloe powder and Rhizoma Dioscoreae powder is 50-80:50-20.
5. use Aloe according to claim 1, Rhizoma Dioscoreae are prepared the method for the treatment of diabetes injection liquid injectable powder, it is characterized in that: the weight portion proportioning of described Aloe powder and Rhizoma Dioscoreae powder is 50-60:50-40.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106581497A (en) * | 2017-01-19 | 2017-04-26 | 湖州师范学院 | Traditional Chinese medicinal composition for treating diabetes |
| CN109043306A (en) * | 2018-08-03 | 2018-12-21 | 湖北文理学院 | Color protection liquid and color protection method for fresh-cut Chinese yam |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103463377A (en) * | 2013-09-09 | 2013-12-25 | 开封九福来生物技术有限公司 | Method for preparing aloe freeze-dried powder injection by using aloe powder |
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- 2013-12-27 CN CN201310731324.8A patent/CN103705696A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103463377A (en) * | 2013-09-09 | 2013-12-25 | 开封九福来生物技术有限公司 | Method for preparing aloe freeze-dried powder injection by using aloe powder |
Non-Patent Citations (2)
| Title |
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| 张云常等: "中药治疗糖尿病的某些研究进展", 《中国医药导刊》, vol. 8, no. 3, 31 December 2006 (2006-12-31) * |
| 王资生: "芦荟山药酸奶的研制", 《食品科学》, vol. 28, no. 9, 31 December 2007 (2007-12-31) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106581497A (en) * | 2017-01-19 | 2017-04-26 | 湖州师范学院 | Traditional Chinese medicinal composition for treating diabetes |
| CN109043306A (en) * | 2018-08-03 | 2018-12-21 | 湖北文理学院 | Color protection liquid and color protection method for fresh-cut Chinese yam |
| CN109043306B (en) * | 2018-08-03 | 2021-08-03 | 湖北文理学院 | Color protection liquid and color protection method for fresh-cut Chinese yam |
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