CN103695479B - 一种高***藜芦醇的制备方法 - Google Patents
一种高***藜芦醇的制备方法 Download PDFInfo
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- CN103695479B CN103695479B CN201310725682.8A CN201310725682A CN103695479B CN 103695479 B CN103695479 B CN 103695479B CN 201310725682 A CN201310725682 A CN 201310725682A CN 103695479 B CN103695479 B CN 103695479B
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- Prior art keywords
- extraction
- enzymolysis
- resveratrol
- crude product
- alcohol
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- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 title claims abstract description 13
- 235000021283 resveratrol Nutrition 0.000 title claims abstract description 13
- 229940016667 resveratrol Drugs 0.000 title claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000000605 extraction Methods 0.000 claims abstract description 34
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000012043 crude product Substances 0.000 claims abstract description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 12
- RHMXXJGYXNZAPX-UHFFFAOYSA-N emodin Chemical compound C1=C(O)C=C2C(=O)C3=CC(C)=CC(O)=C3C(=O)C2=C1O RHMXXJGYXNZAPX-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002904 solvent Substances 0.000 claims abstract description 12
- 239000006227 byproduct Substances 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000004440 column chromatography Methods 0.000 claims abstract description 6
- 238000005406 washing Methods 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 3
- 241001648835 Polygonum cuspidatum Species 0.000 claims abstract 2
- 235000018167 Reynoutria japonica Nutrition 0.000 claims abstract 2
- 229940079593 drug Drugs 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims description 32
- 230000008569 process Effects 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 19
- 150000001875 compounds Chemical class 0.000 claims description 16
- 239000000470 constituent Substances 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 7
- 230000003014 reinforcing effect Effects 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 108010059820 Polygalacturonase Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- -1 concentrated Substances 0.000 claims description 5
- 239000013078 crystal Substances 0.000 claims description 5
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000003808 methanol extraction Methods 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 235000019441 ethanol Nutrition 0.000 abstract 3
- 239000010282 Emodin Substances 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000002425 crystallisation Methods 0.000 abstract 1
- 230000008025 crystallization Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000000796 flavoring agent Substances 0.000 abstract 1
- 235000019634 flavors Nutrition 0.000 abstract 1
- 238000004537 pulping Methods 0.000 abstract 1
- 235000018991 trans-resveratrol Nutrition 0.000 description 25
- 244000153955 Reynoutria sachalinensis Species 0.000 description 20
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 description 20
- 238000005516 engineering process Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000000543 intermediate Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- HSTZMXCBWJGKHG-UHFFFAOYSA-N (E)-piceid Natural products OC1C(O)C(O)C(CO)OC1OC1=CC(O)=CC(C=CC=2C=CC(O)=CC=2)=C1 HSTZMXCBWJGKHG-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229960003764 polydatin Drugs 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- HSTZMXCBWJGKHG-CUYWLFDKSA-N trans-piceid Polymers O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=CC(\C=C\C=2C=CC(O)=CC=2)=C1 HSTZMXCBWJGKHG-CUYWLFDKSA-N 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 1
- 241000219050 Polygonaceae Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960001701 chloroform Drugs 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
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- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
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CN201310725682.8A CN103695479B (zh) | 2013-12-25 | 2013-12-25 | 一种高***藜芦醇的制备方法 |
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CN201310725682.8A CN103695479B (zh) | 2013-12-25 | 2013-12-25 | 一种高***藜芦醇的制备方法 |
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CN103695479A CN103695479A (zh) | 2014-04-02 |
CN103695479B true CN103695479B (zh) | 2015-05-13 |
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Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105250487A (zh) * | 2015-10-19 | 2016-01-20 | 潘勤霞 | 一种提取茄子黄酮的方法 |
CN107300590A (zh) * | 2017-05-26 | 2017-10-27 | 广州中医药大学 | 一种从虎杖中提取虎杖苷和白藜芦醇的hplc检测方法 |
CN108821953A (zh) * | 2018-05-30 | 2018-11-16 | 上海华堇生物技术有限责任公司 | 一种天然白藜芦醇的精制纯化方法 |
CN111909005A (zh) * | 2020-08-11 | 2020-11-10 | 唐山市食品药品综合检验检测中心(唐山市检验检测研究院) | 一种花生皮渣白藜芦醇的制备方法 |
CN112552147A (zh) * | 2020-11-24 | 2021-03-26 | 菏泽学院 | 一种从丹凤牡丹籽粕中提取白藜芦醇的制备方法 |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN101338327B (zh) * | 2008-08-13 | 2011-03-23 | 长沙华诚生物科技有限公司 | 从虎杖中提取纯度98%以上的白藜芦醇的工艺 |
CN101643754A (zh) * | 2009-08-31 | 2010-02-10 | 三明华健生物工程有限公司 | 从虎杖中提取制备高***藜芦醇新工艺 |
CN102080108A (zh) * | 2010-12-15 | 2011-06-01 | 云南弗蓝替生物工程有限公司 | 用真空酶解技术提取虎杖中大黄素、虎杖甙及白藜芦醇的方法 |
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GR01 | Patent grant | ||
CB03 | Change of inventor or designer information |
Inventor after: Xie Limian Inventor before: Li Zhiping |
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CB03 | Change of inventor or designer information | ||
TR01 | Transfer of patent right |
Effective date of registration: 20171225 Address after: 221600 Xuzhou City, Peixian, Jiangsu Province, Han Xiang Road East, Yu Jia clothing North Patentee after: JIANGSU GUILIU ANIMAL HUSBANDRY CO.,LTD. Address before: 416000 Hunan, Xiangxi Tujia and Miao Autonomous Prefecture, Jishou City People's road, No. 120 Patentee before: Jishou University |
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Address after: 221600 Dongyujia Costume North of Hanxiang Road, Peixian County, Xuzhou City, Jiangsu Province Patentee after: JIANGSU GUILIU ANIMAL HUSBANDRY GROUP Co.,Ltd. Address before: 221600 Jiangsu, Xuzhou, Peixian, Han Xiang Road East, Yu Jia dress North Patentee before: JIANGSU GUILIU ANIMAL HUSBANDRY CO.,LTD. |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150513 |
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CF01 | Termination of patent right due to non-payment of annual fee |