CN103695325A - Candida tropicalis and method for preparing L-valine through microbiological method - Google Patents
Candida tropicalis and method for preparing L-valine through microbiological method Download PDFInfo
- Publication number
- CN103695325A CN103695325A CN201310681782.5A CN201310681782A CN103695325A CN 103695325 A CN103695325 A CN 103695325A CN 201310681782 A CN201310681782 A CN 201310681782A CN 103695325 A CN103695325 A CN 103695325A
- Authority
- CN
- China
- Prior art keywords
- valine
- high purity
- candida tropicalis
- thalline
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000222178 Candida tropicalis Species 0.000 title claims description 14
- 229960004295 valine Drugs 0.000 title abstract 5
- 238000013048 microbiological method Methods 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims abstract description 7
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims abstract description 6
- 230000000813 microbial effect Effects 0.000 claims abstract description 6
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims abstract description 5
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 39
- 239000004474 valine Substances 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 18
- 239000013078 crystal Substances 0.000 claims description 13
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- 238000002425 crystallisation Methods 0.000 claims description 6
- 230000008025 crystallization Effects 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 235000004279 alanine Nutrition 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 239000005864 Sulphur Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 244000005700 microbiome Species 0.000 abstract description 4
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 abstract 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 abstract 1
- 229930182844 L-isoleucine Natural products 0.000 abstract 1
- 235000019454 L-leucine Nutrition 0.000 abstract 1
- 239000004395 L-leucine Substances 0.000 abstract 1
- 229960003767 alanine Drugs 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 229960000310 isoleucine Drugs 0.000 abstract 1
- 229960003136 leucine Drugs 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 235000014393 valine Nutrition 0.000 description 36
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000000967 suction filtration Methods 0.000 description 6
- 150000003680 valines Chemical class 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 3
- 238000004811 liquid chromatography Methods 0.000 description 3
- 229940066779 peptones Drugs 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- KIWBPDUYBMNFTB-UHFFFAOYSA-N Ethyl hydrogen sulfate Chemical compound CCOS(O)(=O)=O KIWBPDUYBMNFTB-UHFFFAOYSA-N 0.000 description 2
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 2
- 102000013275 Somatomedins Human genes 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000001311 chemical methods and process Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 239000012716 precipitator Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 125000003412 L-alanyl group Chemical group [H]N([H])[C@@](C([H])([H])[H])(C(=O)[*])[H] 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- -1 through concentrated Chemical compound 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention disclosed a method for preparing high-purity L-valine through the steps of screening microorganism through mutation, carrying out heteroacid growth of a microbial nutritional deficient strain in an L-valine fermentation broth through the fermentation broth, degrading heteroacid (mainly including L-alanine, L-isoleucine and L-leucine) in the L-valine fermentation broth after growth of the strain, and purifying L-valine from the fermentation broth, and belongs to the field of fermentation engineering. The method disclosed by the invention has the characteristics of simple production process, mild conditions, no pollution in production process and high product purity.
Description
Technical field
The present invention relates to field of fermentation engineering, more particularly, relate to a kind of method that microorganism impurity removal method is prepared high purity Valine.
Background technology
High purity L-α-amino-isovaleric acid is in the market mainly by fermentative Production main raw material, by other the assorted amino acid in the precipitator method or the separated Valine of chromatography, Valine, through concentrated, alcohol is analysed, desalination, hydrolysis, refining, dry and obtain high purity Valine.High purity-the α-amino-isovaleric acid that adopts chemical method to produce, can adopt a large amount of chemical reagent in production process, and not only cost is high, and yield is low, can produce a large amount of organism, inorganic salt, solid waste and organic waste water in production process simultaneously, unfriendly to environment.And the biotechnology adopting is the production technique that country encourages class.
In addition, high purity Valine is in the market mainly to obtain content greatly about 90% Valine by fermentation method, the heteroacid mainly containing in finished product is ALANINE, ILE and L-Leu, the main method of removing at present heteroacid is the precipitator method, with chemical reagent, precipitate heteroacid, restore into Valine, obtain high purity Valine.Japan adopts this patent to produce.While adopting the high purity Valine that chemical method produces, can adopt a large amount of chemical reagent, not only cost is high, can produce a large amount of organism and organic waste water in production process simultaneously, unfriendly to environment.
Summary of the invention
The object of the invention is to provide a kind of L of take-α-amino-isovaleric acid as the biological method of raw material production high purity L-α-amino-isovaleric acid, has that production process is simple, a mild condition, production process is pollution-free, product purity is high feature.
In order to achieve the above object, first the present invention provides a kind of candida tropicalis (Saccharomyces cerevisiae) DLPU-IL
-, deposit number CGMCC NO.7784.
The present invention also provides above-mentioned candida tropicalis to prepare the application of high purity Valine.
Specifically, a kind of microbial method of the present invention is prepared the method for high purity Valine, by candida tropicalis DLPU-IL
-join cultivation and fermentation in Valine fermented liquid, run out of L-Leu, ALANINE, ILE in Valine; Remove in the fermentation clear liquid after thalline and add activated carbon decolorizing, remove by filter afterwards gac, destainer is concentrated, crystallization, and with alcohol crystal and rinsing, the centrifugal wet crystal of high purity Valine that obtains; After oven dry, obtain high purity Valine finished product.Wherein, described Candida tropicalis body is selected the candida tropicalis (Saccharomyces cerevisiae) of deposit number CGMCC NO.7784.
Under optimal way, microbial method is prepared the method for high purity Valine, comprises the steps:
S1, in volume mass per-cent preparation slant medium: 0.5% glucose, 1.0% peptone, 1.0% yeast extract paste, 2% agar powder; 121 ℃ of sterilizing 30min.
S2, by candida strain DLPU-IL
-access in described slant medium under 30 ℃, the condition of 250～350rpm and cultivate 24～48h, centrifuging and taking thalline;
S3, in volume mass per-cent preparation fermention medium: Valine 5～10%, peptone 0.2～2.0%, yeast extract paste 0.2～1.5%;
S4, thalline is added in described fermention medium, under 30 ℃, 250～350rpm, react, in reaction process, every 2～4 hours, drip 10% sulphur acid for adjusting pH, it is maintained between 6.0～6.2, when within 64～72 hours, pH no longer rises, finish fermentation;
S5, the centrifugal thalline of removing, add 100 ℃ of gacs decolouring, 30～40min in supernatant liquor, filter, concentrated, crystallization, oven dry obtain high purity Valine.
The strain auxotrophic mutant CGMCC No.7784 that a strain candida tropicalis arrives with ethyl sulfate mutagenic and breeding as starting strain is take in utilization of the present invention, the leucine that this bacterial strain can be take in Valine fermented liquid is somatomedin, ALANINE and the ILE that can degrade in Valine fermented liquid, produce high purity Valine by operations such as fermented liquid degerming, fermentation clear liquid decolouring, decolouring destainer concentrating under reduced pressure, crystallizations simultaneously.The chemical process of abandoning tradition of the present invention, not with an organic solvent, produces without waste water, is a kind of green production process.
Preservation explanation
The preservation information of the biological material specimens the present invention relates to: ginseng certificate microorganism (strain) be, DLPU-IL
-classification And Nomenclature is candiyeast, (abbreviation CGMCC) preservation in June, 2013 13You China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number CGMCC NO.7784.CGMCC address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
Embodiment
The present invention be take candida tropicalis as starting strain, adopts ethyl sulfate induced-mutation technique to screen a strain auxotrophic strain preserving number CGMCC No.7784(hereinafter to be referred as 7784), this bacterial strain DLPU-IL
-the heteroacid L-Leu that can take in Valine is somatomedin, simultaneously the impurity amino acid such as the ALANINE in degradable fermented substratum and ILE, by extraction, obtain high purity Valine.Detailed process is as follows:
Preparation slant medium (composition in substratum is in volume mass per-cent): 0.5% glucose, 1.0% peptone, 1.0% yeast extract paste, 2% agar powder, 121 ℃ of sterilizing 30min.
Bacterial classification is accessed in above-mentioned substratum under 30 ℃, the condition of 250～350rpm and cultivates 24～48h, centrifuging and taking thalline.
In volume mass per-cent preparation fermention medium: 5～10%L-α-amino-isovaleric acid, peptone 0.2～2.0%, yeast extract paste 0.2～1.5%.
Thalline is joined in fermention medium (Valine fermented liquid), under 30 ℃, 250～350rpm, react, in reaction process, every 2～4 hours, drip 10% sulphur acid for adjusting pH, it is maintained between 6.0～6.2,4～5 days fermentation ends.The centrifugal thalline of removing, adds 100 ℃ of gacs decolouring, 30～40min in supernatant liquor, filter, concentrated, crystallization, oven dry obtain high purity pharmaceutical grade standard Valine (Valine content 98.5～100%).
Embodiment 1:
First bacterial classification 7784 is cultivated on slant medium, cultivated 24 hours for 30 ℃.
Preparation fermentative medium formula, takes 8.5 grams of Valines, 2 grams of peptones, 1.0 grams of yeast extract pastes, and natural pH is contained in tap water constant volume to 100 milliliter in the Erlenmeyer flask of 1000 milliliters, and 121 ℃ of sterilizings 20 minutes are standby.
The bacterium of 7784 activation is inoculated in and is equipped with in 100ml fermention medium, 32 ℃ of cultivations, shaking speed is 260 rpms, ferments after 24 hours, to start stream and add 10% hydrochloric acid, the point sample that ferments after 66 hours is analyzed in fermented liquid and is existed without heteroacid, fermentation ends.
85 ℃ of sterilizings of fermented liquid 32 minutes, with after whizzer bactofugation, 100 milliliters of 95 ℃ of decolouring 30min of gac that add 1.2 grams of supernatant liquor, suction filtration, removes gac while hot, gets decolouring clear liquid concentrating under reduced pressure (vacuum tightness is-0.09MPa) and stops after having crystal to occur, be cooled to 28 ℃, suction filtration.50 milliliters of rinsings of ethanol 65 minutes for crystal, the centrifugal crystal that obtains, dries 3 hours for 95 ℃.Obtain 8.0 grams of high purity Valines.Yield is 94.12%.Purity through high effective liquid chromatography for measuring Valine is 99.12%.
Embodiment 2:
First bacterial classification 7784 is cultivated on slant medium, cultivated 24 hours for 30 ℃.
Preparation fermentative medium formula, takes 17 grams of Valines, 4.0 grams of peptones, 2.0 grams of yeast extract pastes, and natural pH is contained in tap water constant volume to 200 milliliter in the Erlenmeyer flask of 3000 milliliters, and 121 ℃ of sterilizings 20 minutes are standby.
The bacterium of 7784 activation is inoculated in dress 200ml fermention medium, 33 ℃ of cultivations, shaking speed is 250 rpms, ferment and after 24 hours, start stream and add 10% hydrochloric acid, every 8 hours stream, add once, pH maintains between 6.5～7.0, and the point sample after 68 hours that ferments is analyzed in fermented liquid without heteroacid existence, fermentation ends.
85 ℃ of sterilizings of fermented liquid 35 minutes, with after whizzer bactofugation, 200 milliliters of 90 ℃ of decolouring 35min of gac that add 3 grams of supernatant liquor, suction filtration, removes gac while hot, gets decolouring clear liquid concentrating under reduced pressure (vacuum tightness is-0.09MPa) and stops after having crystal to occur, be cooled to 30 ℃, suction filtration.100 milliliters of rinsings of ethanol 75 minutes for crystal, the centrifugal crystal that obtains, dries 4 hours for 93 ℃.Obtain 15.6 grams of Valines.Yield is 92%.Threshold value through high effective liquid chromatography for measuring Valine is 98.88%.
Embodiment 3:
First bacterial classification 7784 is cultivated on slant medium, cultivated 24 hours for 30 ℃.
Preparation fermentative medium formula, takes 34 grams of Valines, 8 grams of peptones, 4.0 grams of yeast extract pastes, and natural pH is contained in tap water constant volume to 400 milliliter in the Erlenmeyer flask of 3000 milliliters, and 121 ℃ of sterilizings 20 minutes are standby.
The bacterium of 7784 activation is inoculated in dress 400ml fermention medium, 34 ℃ of cultivations, shaking speed is 285 rpms, ferment and after 24 hours, start stream and add 10% hydrochloric acid, every 8 hours stream, add once, pH maintains between 6.5-7.0, and the point sample after 70 hours that ferments is analyzed in fermented liquid without heteroacid existence, fermentation ends.
90 ℃ of sterilizings of fermented liquid 30 minutes, with after whizzer bactofugation, 400 milliliters of 98 ℃ of decolouring 35min of gac that add 7.2 grams of supernatant liquor, suction filtration, removes gac while hot, gets decolouring clear liquid concentrating under reduced pressure (vacuum tightness is-0.09MPa) and stops after having crystal to occur, be cooled to 29 ℃, suction filtration.100 milliliters of rinsings of ethanol 65 minutes for crystal, the centrifugal crystal that obtains, dries 2.5 hours for 92 ℃.Obtain 32 grams of Valines.Yield is 94.12%.Purity through high effective liquid chromatography for measuring Valine is 99.05%.
The chemical process of abandoning tradition of the present invention, not with an organic solvent, produces without waste water, is a kind of green production process.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; according to technical scheme of the present invention and inventive concept thereof, be equal to replacement or changed, within all should being encompassed in protection scope of the present invention.
Claims (4)
1. candida tropicalis (Saccharomyces cerevisiae) DLPU-IL-, is characterized in that deposit number CGMCC NO.7784.
2. described in claim 1, candida tropicalis is prepared the application of high purity Valine.
3. microbial method is prepared a method for high purity Valine, it is characterized in that, candida tropicalis DLPU-IL-joins cultivation and fermentation in Valine fermented liquid, runs out of L-Leu, ALANINE, ILE in Valine;
Remove in the fermentation clear liquid after thalline and add activated carbon decolorizing, remove by filter afterwards gac, destainer is concentrated, crystallization, and with alcohol crystal and rinsing, the centrifugal wet crystal of high purity Valine that obtains; After oven dry, obtain high purity Valine finished product.
Wherein, described Candida tropicalis body is selected the candida tropicalis (Saccharomyces cerevisiae) of deposit number CGMCC NO.7784.
4. microbial method is prepared the method for high purity Valine according to claim 3, it is characterized in that comprising the steps:
S1, in volume mass per-cent preparation slant medium: 0.5% glucose, 1.0% peptone, 1.0% yeast extract paste, 2% agar powder; 121 ℃ of sterilizing 30min.
S2, by candida strain DLPU-IL
-access in described slant medium under 30 ℃, the condition of 250～350rpm and cultivate 24～48h, centrifuging and taking thalline;
S3, in volume mass per-cent preparation fermention medium: Valine 5～10%, peptone 0.2～2.0%, yeast extract paste 0.2～1.5%;
S4, thalline is added in described fermention medium, under 30 ℃, 250～350rpm, react, in reaction process, every 2～4 hours, drip 10% sulphur acid for adjusting pH, it is maintained between 6.0～6.2, when within 64～72 hours, pH no longer rises, finish fermentation;
S5, the centrifugal thalline of removing, add 100 ℃ of gacs decolouring, 30～40min in supernatant liquor, filter, concentrated, crystallization, oven dry obtain high purity Valine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310681782.5A CN103695325B (en) | 2013-12-12 | 2013-12-12 | A kind of candida tropicalis and a kind of microbial method prepare the method for Valine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310681782.5A CN103695325B (en) | 2013-12-12 | 2013-12-12 | A kind of candida tropicalis and a kind of microbial method prepare the method for Valine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103695325A true CN103695325A (en) | 2014-04-02 |
| CN103695325B CN103695325B (en) | 2016-04-13 |
Family
ID=50356989
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310681782.5A Active CN103695325B (en) | 2013-12-12 | 2013-12-12 | A kind of candida tropicalis and a kind of microbial method prepare the method for Valine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103695325B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486894A (en) * | 2019-01-11 | 2019-03-19 | 内蒙古拜克生物有限公司 | A kind of production method of Valine |
| CN110372773A (en) * | 2019-07-18 | 2019-10-25 | 大连医诺生物股份有限公司 | The production method of high-purity glutamine dipeptide |
| CN110684785A (en) * | 2018-07-06 | 2020-01-14 | 上海凯赛生物技术股份有限公司 | Long-chain dibasic acid of low-content low-carbon-chain long-chain dibasic acid heteropolyacid and preparation method thereof |
| CN112239730A (en) * | 2019-07-18 | 2021-01-19 | 大连医诺生物股份有限公司 | Candida tropicalis mutant strain and application thereof in BCAA preparation |
| US12018311B2 (en) | 2018-07-06 | 2024-06-25 | Cibt America Inc. | Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101631871A (en) * | 2007-02-22 | 2010-01-20 | 味之素株式会社 | Method of producing L-amino acid |
| WO2012136506A2 (en) * | 2011-04-04 | 2012-10-11 | Evonik Degussa Gmbh | Microorganism and processes for the fermentative production of an organo-chemical compound |
| CN103397056A (en) * | 2013-08-15 | 2013-11-20 | 廊坊梅花生物技术开发有限公司 | Method for preparing L-amino acid |
-
2013
- 2013-12-12 CN CN201310681782.5A patent/CN103695325B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101631871A (en) * | 2007-02-22 | 2010-01-20 | 味之素株式会社 | Method of producing L-amino acid |
| WO2012136506A2 (en) * | 2011-04-04 | 2012-10-11 | Evonik Degussa Gmbh | Microorganism and processes for the fermentative production of an organo-chemical compound |
| WO2012136506A3 (en) * | 2011-04-04 | 2012-12-06 | Evonik Degussa Gmbh | Microorganism and processes for the fermentative production of an organo-chemical compound |
| CN103397056A (en) * | 2013-08-15 | 2013-11-20 | 廊坊梅花生物技术开发有限公司 | Method for preparing L-amino acid |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684785A (en) * | 2018-07-06 | 2020-01-14 | 上海凯赛生物技术股份有限公司 | Long-chain dibasic acid of low-content low-carbon-chain long-chain dibasic acid heteropolyacid and preparation method thereof |
| CN110684785B (en) * | 2018-07-06 | 2023-08-08 | 上海凯赛生物技术股份有限公司 | Long-chain dibasic acid with low content of low-carbon-chain long-chain dibasic acid hetero acid and preparation method thereof |
| US12018311B2 (en) | 2018-07-06 | 2024-06-25 | Cibt America Inc. | Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof |
| CN109486894A (en) * | 2019-01-11 | 2019-03-19 | 内蒙古拜克生物有限公司 | A kind of production method of Valine |
| CN110372773A (en) * | 2019-07-18 | 2019-10-25 | 大连医诺生物股份有限公司 | The production method of high-purity glutamine dipeptide |
| CN112239730A (en) * | 2019-07-18 | 2021-01-19 | 大连医诺生物股份有限公司 | Candida tropicalis mutant strain and application thereof in BCAA preparation |
| CN112239730B (en) * | 2019-07-18 | 2022-01-14 | 大连医诺生物股份有限公司 | Candida tropicalis mutant strain and application thereof in BCAA preparation |
| CN110372773B (en) * | 2019-07-18 | 2022-06-10 | 大连医诺生物股份有限公司 | Production method of high-purity glutamine dipeptide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103695325B (en) | 2016-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2020119828A1 (en) | Bacillus clausii and method for producing ectoine using same | |
| CN103695325B (en) | A kind of candida tropicalis and a kind of microbial method prepare the method for Valine | |
| CN110760452B (en) | Lu's combined yeast and application thereof in soybean paste fermentation | |
| CN100390271C (en) | Method of producing xylosic alcohol and its special baterial strain | |
| CN103088089B (en) | Method for fermenting acarbose | |
| CN112094750B (en) | Rhizopus oryzae for producing ergothioneine | |
| CN110373346B (en) | Bacillus subtilis sp.A-5 and application thereof | |
| CN101338291B (en) | Method for preparing coronatine and special strain thereof | |
| CN103373901B (en) | Method for extracting erythritol from erythritol mother liquor and special barm strain for erythritol | |
| CN104152522A (en) | Method for preparing wheat active peptide from mixed bacterium solid state fermentation gluten powder | |
| CN107446868B (en) | One plant of Methylotrophic bacillus and its degradation of feather produce the application of oligopeptides | |
| CN102220390A (en) | Method for preparing citrulline by combining arginine fermentation and enzymatic conversion | |
| CN108823127A (en) | For the bacterial strain of α-amylase Producer and the isolation and purification method of alpha-amylase | |
| CN106434443B (en) | A kind of production technology of Sodium Hyaluronate | |
| CN102816751B (en) | High-activity chitosanase and preparation method thereof | |
| CN103468606A (en) | Klebsiella oxytoca and application thereof in allitol production | |
| CN103540631B (en) | Method for producing sophorolipid through fermentation by taking cottonseed molasses and cottonseed oil as substrates | |
| CN109022329B (en) | A bipolaris strain for producing biosurfactant | |
| CN102492634A (en) | High-temperature resistant yeast and application thereof | |
| CN112553259A (en) | Method for producing microbial flocculant by using Bacillus licheniformis | |
| CN102382787B (en) | Corynebacterium pekinense and application thereof | |
| CN102234673B (en) | Fermentation culture medium and fermentation method for producing pentostatin by fermentation of streptomyces antibioticus | |
| CN102634463B (en) | Saccharomycete producing xylitol and applicaton of saccharomycete | |
| CN106010992B (en) | The method that saccharomyces cerevisiae and saccharomyces cerevisiae prepare succinic acid | |
| CN106319004A (en) | Fermentation culture medium capable of enhancing nosiheptide yield and culture method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhang Chunzhi Inventor after: Pu Junping Inventor after: Yu Ying Inventor after: Chen Li Inventor after: Chen Ming Inventor after: Lu Ming Inventor before: Zhang Chunzhi Inventor before: Pu Junping Inventor before: Yu Ying Inventor before: Chen Li Inventor before: Chen Ming Inventor before: Zu Guoren |
|
| COR | Change of bibliographic data | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231009 Address after: No. 49, Dalian Economic and Technological Development Zone, Dalian City, Liaoning Province, 116600 Dalian Yinuo Patentee after: INNOBIO Corp.,Ltd. Patentee after: Heilongjiang nuoshu Biotechnology Co.,Ltd. Address before: 116034 No. 1 light industry garden, Ganjingzi District, Liaoning, Dalian Patentee before: DALIAN POLYTECHNIC University |