CN103695325A - Candida tropicalis and method for preparing L-valine through microbiological method - Google Patents
Candida tropicalis and method for preparing L-valine through microbiological method Download PDFInfo
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- CN103695325A CN103695325A CN201310681782.5A CN201310681782A CN103695325A CN 103695325 A CN103695325 A CN 103695325A CN 201310681782 A CN201310681782 A CN 201310681782A CN 103695325 A CN103695325 A CN 103695325A
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- CN
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- Prior art keywords
- valine
- high purity
- candida tropicalis
- thalline
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 24
- 241000222178 Candida tropicalis Species 0.000 title claims description 14
- 229960004295 valine Drugs 0.000 title abstract 5
- 238000013048 microbiological method Methods 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims abstract description 7
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims abstract description 6
- 230000000813 microbial effect Effects 0.000 claims abstract description 6
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims abstract description 5
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 39
- 239000004474 valine Substances 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 18
- 239000013078 crystal Substances 0.000 claims description 13
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- 238000002425 crystallisation Methods 0.000 claims description 6
- 230000008025 crystallization Effects 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 235000004279 alanine Nutrition 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 239000005864 Sulphur Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 244000005700 microbiome Species 0.000 abstract description 4
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 abstract 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 abstract 1
- 229930182844 L-isoleucine Natural products 0.000 abstract 1
- 235000019454 L-leucine Nutrition 0.000 abstract 1
- 239000004395 L-leucine Substances 0.000 abstract 1
- 229960003767 alanine Drugs 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 229960000310 isoleucine Drugs 0.000 abstract 1
- 229960003136 leucine Drugs 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 235000014393 valine Nutrition 0.000 description 36
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000000967 suction filtration Methods 0.000 description 6
- 150000003680 valines Chemical class 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 3
- 238000004811 liquid chromatography Methods 0.000 description 3
- 229940066779 peptones Drugs 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- KIWBPDUYBMNFTB-UHFFFAOYSA-N Ethyl hydrogen sulfate Chemical compound CCOS(O)(=O)=O KIWBPDUYBMNFTB-UHFFFAOYSA-N 0.000 description 2
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 2
- 102000013275 Somatomedins Human genes 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000001311 chemical methods and process Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 239000012716 precipitator Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 125000003412 L-alanyl group Chemical group [H]N([H])[C@@](C([H])([H])[H])(C(=O)[*])[H] 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- -1 through concentrated Chemical compound 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201310681782.5A CN103695325B (en) | 2013-12-12 | 2013-12-12 | A kind of candida tropicalis and a kind of microbial method prepare the method for Valine |
Applications Claiming Priority (1)
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CN201310681782.5A CN103695325B (en) | 2013-12-12 | 2013-12-12 | A kind of candida tropicalis and a kind of microbial method prepare the method for Valine |
Publications (2)
Publication Number | Publication Date |
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CN103695325A true CN103695325A (en) | 2014-04-02 |
CN103695325B CN103695325B (en) | 2016-04-13 |
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CN201310681782.5A Active CN103695325B (en) | 2013-12-12 | 2013-12-12 | A kind of candida tropicalis and a kind of microbial method prepare the method for Valine |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109486894A (en) * | 2019-01-11 | 2019-03-19 | 内蒙古拜克生物有限公司 | A kind of production method of Valine |
CN110372773A (en) * | 2019-07-18 | 2019-10-25 | 大连医诺生物股份有限公司 | The production method of high-purity glutamine dipeptide |
CN110684785A (en) * | 2018-07-06 | 2020-01-14 | 上海凯赛生物技术股份有限公司 | Long-chain dibasic acid of low-content low-carbon-chain long-chain dibasic acid heteropolyacid and preparation method thereof |
CN112239730A (en) * | 2019-07-18 | 2021-01-19 | 大连医诺生物股份有限公司 | Candida tropicalis mutant strain and application thereof in BCAA preparation |
US12018311B2 (en) | 2018-07-06 | 2024-06-25 | Cibt America Inc. | Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101631871A (en) * | 2007-02-22 | 2010-01-20 | 味之素株式会社 | Method of producing L-amino acid |
WO2012136506A2 (en) * | 2011-04-04 | 2012-10-11 | Evonik Degussa Gmbh | Microorganism and processes for the fermentative production of an organo-chemical compound |
CN103397056A (en) * | 2013-08-15 | 2013-11-20 | 廊坊梅花生物技术开发有限公司 | Method for preparing L-amino acid |
-
2013
- 2013-12-12 CN CN201310681782.5A patent/CN103695325B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101631871A (en) * | 2007-02-22 | 2010-01-20 | 味之素株式会社 | Method of producing L-amino acid |
WO2012136506A2 (en) * | 2011-04-04 | 2012-10-11 | Evonik Degussa Gmbh | Microorganism and processes for the fermentative production of an organo-chemical compound |
WO2012136506A3 (en) * | 2011-04-04 | 2012-12-06 | Evonik Degussa Gmbh | Microorganism and processes for the fermentative production of an organo-chemical compound |
CN103397056A (en) * | 2013-08-15 | 2013-11-20 | 廊坊梅花生物技术开发有限公司 | Method for preparing L-amino acid |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110684785A (en) * | 2018-07-06 | 2020-01-14 | 上海凯赛生物技术股份有限公司 | Long-chain dibasic acid of low-content low-carbon-chain long-chain dibasic acid heteropolyacid and preparation method thereof |
CN110684785B (en) * | 2018-07-06 | 2023-08-08 | 上海凯赛生物技术股份有限公司 | Long-chain dibasic acid with low content of low-carbon-chain long-chain dibasic acid hetero acid and preparation method thereof |
US12018311B2 (en) | 2018-07-06 | 2024-06-25 | Cibt America Inc. | Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof |
CN109486894A (en) * | 2019-01-11 | 2019-03-19 | 内蒙古拜克生物有限公司 | A kind of production method of Valine |
CN110372773A (en) * | 2019-07-18 | 2019-10-25 | 大连医诺生物股份有限公司 | The production method of high-purity glutamine dipeptide |
CN112239730A (en) * | 2019-07-18 | 2021-01-19 | 大连医诺生物股份有限公司 | Candida tropicalis mutant strain and application thereof in BCAA preparation |
CN112239730B (en) * | 2019-07-18 | 2022-01-14 | 大连医诺生物股份有限公司 | Candida tropicalis mutant strain and application thereof in BCAA preparation |
CN110372773B (en) * | 2019-07-18 | 2022-06-10 | 大连医诺生物股份有限公司 | Production method of high-purity glutamine dipeptide |
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Publication number | Publication date |
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CN103695325B (en) | 2016-04-13 |
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Inventor after: Zhang Chunzhi Inventor after: Pu Junping Inventor after: Yu Ying Inventor after: Chen Li Inventor after: Chen Ming Inventor after: Lu Ming Inventor before: Zhang Chunzhi Inventor before: Pu Junping Inventor before: Yu Ying Inventor before: Chen Li Inventor before: Chen Ming Inventor before: Zu Guoren |
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Effective date of registration: 20231009 Address after: No. 49, Dalian Economic and Technological Development Zone, Dalian City, Liaoning Province, 116600 Dalian Yinuo Patentee after: INNOBIO Corp.,Ltd. Patentee after: Heilongjiang nuoshu Biotechnology Co.,Ltd. Address before: 116034 No. 1 light industry garden, Ganjingzi District, Liaoning, Dalian Patentee before: DALIAN POLYTECHNIC University |