CN103691498A - Liquid-moving machine and method of moving liquid by same - Google Patents
Liquid-moving machine and method of moving liquid by same Download PDFInfo
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- CN103691498A CN103691498A CN201410004737.0A CN201410004737A CN103691498A CN 103691498 A CN103691498 A CN 103691498A CN 201410004737 A CN201410004737 A CN 201410004737A CN 103691498 A CN103691498 A CN 103691498A
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Abstract
The invention provides a liquid-moving machine and a method of moving liquid by the same. The liquid-moving machine comprises a base, a vertical column arranged on one side of the base, a Z-axis motor arranged on the vertical column, a first lead screw arranged on an output shaft of the Z-axis motor, a slide block arranged on the first lead screw, a liquid-moving mechanism fixedly installed on the slide block and an electromagnetic positioning device which is arranged on the base and is electrically connected with the Z-axis motor, wherein the liquid-moving mechanism comprises a piston head connecting and fixing frame connected with the slide block, a piston motor assembled on the piston head connecting and fixing frame, a second lead screw connected with an output shaft of the piston motor, a piston fixing plate connected with the second lead screw, a plurality of pistons assembled on the piston fixing plate and piston cylinders assembled on the pistons. A plurality of insertion nozzles are arranged at the lower ends of the piston cylinders by corresponding to the pistons, a sealed convex ring and a positioning convex ring are arranged at the lower end of each insertion nozzle, a push rod is arranged on the piston fixing plate, and a sucker withdrawing push plate is arranged at the lower end of the push rod. The sucker withdrawing push plate is arranged below each piston cylinder and above the positioning convex ring.
Description
Technical field
The present invention relates to medical testing equipment field, relate in particular to a kind of pipettor and use it to move the method for liquid.
Background technology
Microwell plate, comprises the forms such as 96 holes and 384 holes, is reaction vessel conventional in bioengineering and medical test.By there being a lot of apertures above it, can load a lot of test samples and react under the same conditions, therefore in the research of biological and medical science with in applying, be used widely.
Biological various reaction all be unable to do without various solution.Toward adding various reaction solutions in these apertures, be very basic and important work.All the time, biomedical worker relies on piston type charger to microwell plate, to carry out the interpolation of various solution.Its primary structure comprises:
1. sliding plunger: move up and down and cause air pressure change to carry out imbibition hydrojet;
2. adjusting screw(rod): determine that the height that piston moves upward determines that liquid absorption is how many;
3. spring: the height by piston automatic rebounding to regulating nut place;
4. push rod: operator overcomes spring force and promotes piston with finger and moves downward with hydrojet;
5. interrupt: entangle plastics suction nozzle and form airtight environment so that liquid enters suction nozzle;
Piston type charger has single head or 8 or 12 s' form.Adopt operator's finger to overcome the liquid-transfering device that spring force is controlled the form of hydrojet, be called traditional pipettor.
More novel liquid-transfering device does not need operator to point to overcome spring force to control hydrojet, and has adopted Electric Machine Control piston movement position to realize imbibition hydrojet.These liquid-transfering devices to above-mentioned traditional pipettor the 2nd, 3,4 dot structures are adjusted, its structure comprises:
1. sliding plunger: move up and down and cause air pressure change to carry out imbibition hydrojet;
2. motor and screw rod: determine that the movement position of piston is to realize quantitative liquid suction/hydrojet;
3. micro computer: stored routine is also controlled motor movement;
4. button and display screen: operator according to program interface in order to input service information;
5. interrupt: entangle plastics suction nozzle and form airtight environment so that liquid enters suction nozzle.
In prior art, the installation and removal of suction nozzle is all to adopt artificial installation, on suction nozzle being fixed on and being interrupted with hand by operator.There are the following problems for manual installation suction nozzle:
1. manually insert suction nozzle and have risk: manually insert suction nozzle may cause interrupt and suction nozzle between seal bad situation and cause and move liquid failure.Because there is certain difference in the big or small direction that staff is exerted oneself at every turn., more easily there is this situation in 8 or 12 s' product particularly.
2. insert the fatigue of suction nozzle/desorption head: manually insert suction nozzle and need to exert oneself to insert, manually desorption head makes operator's finger tired downwards.Particularly 8 or 12 s' product, needs very large strength just can be seated/decorporate.
Summary of the invention
The object of the invention is to overcome the defect of prior art, the pipettor of a kind of energy Auto-mounting and dismounting suction nozzle is provided.
Another object of the present invention is to provide a kind of method of moving liquid, the method is simple to operate, easy to control and fault rate is low.
For achieving the above object, the invention provides a kind of pipettor, comprise base, be located at base one side column, be located at Z axis motor on column, be located at the first screw mandrel of Z axis motor output shaft, the electromagnetic locator of being located at slide block on the first screw mandrel, being fixedly installed in the pipetting device on slide block and being located on base and being electrically connected with Z axis motor, described pipetting device comprises the piston head Connection Bracket of connection sliding block, be installed in the piston motor on piston head Connection Bracket, the second screw mandrel being connected with the output shaft of piston motor, the piston fixed head being connected with the second screw mandrel, be installed in the several pistons on piston fixed head, be set in the piston/cylinder on piston, the corresponding piston in lower end of piston/cylinder is provided with several interrupting, the described upper end of interrupting is for packed-piston cylinder body, the described lower end of interrupting is for being socketed suction nozzle, the described lower end of interrupting is respectively equipped with the directed bulge loop that is positioned at the sealing bulge loop of below and is positioned at top, on described piston fixed head, be provided with push rod, the lower end of push rod is provided with a desorption push pedal, this desorption push pedal is arranged at piston/cylinder below, and be arranged at the top of the directed bulge loop of interrupting, described electromagnetic locator is provided with plate rail.
The upper end of described piston/cylinder is provided with sealing ring, described piston, sealing ring, piston/cylinder, and interrupt between form seal cavity, described piston moves up and down and makes to produce in seal cavity pressure and change in seal cavity, thereby can promote liquid, enters or discharges and interrupt in the suction nozzle of below socket.
Described interrupting adopts flexible material to make.
Also comprise the touch screen control panel and the operating grip that are electrically connected described Z axis motor, described piston motor and described electromagnetic locator.
Described piston is 96, described in to interrupt be 96.
Described column is provided with the polished rod parallel with the first screw mandrel, and on described slide block, corresponding polished rod is provided with through hole, and described polished rod is located in this through hole.
Described electromagnetic locator comprises the guide rail that is installed on base, the locating piece being located at plate rail on guide rail, being located at the relative both sides of guide rail of sliding, be located in plate rail and the electromagnet of relative positioning piece setting, be located on locating piece and the iron block of electromagnet setting relatively, be located at crashproof rubber cushion in plate rail, be located at the microswitch in plate rail and be located on described locating piece and set screw that corresponding described microswitch arranges, and described electromagnet is electrically connected described Z axis motor; Described electromagnetic locator also comprises to be located in plate rail and to be positioned at the first installation base of guide rail top and to be located at plate rail and to be positioned at the second installation base below guide rail, described electromagnet is located on this first installation base, between this electromagnet and the first installation base, be provided with spring, described crashproof rubber cushion and described microswitch are all fixedly installed on this second installation base.
Be positioned at the distance D 1 that the described set screw of the same side and the distance D 3 of microswitch are less than described electromagnet and described iron block, the distance D 1 of described electromagnet and described iron block is less than the distance D 2 of described crashproof rubber cushion and described locating piece.
The present invention also provides a kind of method of using above-mentioned pipettor to move liquid, comprises the following steps:
Step 4, hand-driven moving Moving plate frame, the source solution disk of making is positioned under suction nozzle, control the running of Z axis motor forward, make suction nozzle position arrive the suction range H2 setting, then control piston motor positive movement sucks liquid to suction nozzle, then by software, automatically control the counter motion of Z axis motor and mention to moving liquid height H 1, complete the sucking action of source solution;
Step 5, hand-driven moving Moving plate frame again, target disc is positioned under suction nozzle, control the running of Z axis motor forward, make suction nozzle position arrive the tapping height H 3 of setting, then the counter motion of control piston motor is expelled in target disc the liquid in suction nozzle, then by software, automatically controlling the counter motion of Z axis motor mentions to moving liquid height H 1, completing and moves liquid.
Also comprise that step 6, control piston motor continue counter motion, drive piston to move downward, discharge air; Hand-driven moving Moving plate frame, make suction nozzle box be positioned at suction nozzle under, control piston fixed head moves downward and promotes push rod and drive a desorption push pedal to produce a downward thrust to suction nozzle downwards, makes suction nozzle depart to interrupt and falls in suction nozzle box, completes thus the dismounting of suction nozzle.
Beneficial effect of the present invention: pipettor of the present invention, by a Z axis motor is set, is connected with Z axis motor pipetting device by the first screw mandrel, pipetting device can be under the drive of Z axis motor, realize Auto-mounting suction nozzle, easy to operate succinct, to install firmly, workload is little; Pipettor of the present invention, by a desorption push pedal is set, can be realized desorption head automatically, greatly reduces experimenter's workload; Adopting 96 pistons to move liquid can efficiently and reliably work, and without operator, pays very large muscle power, greatly reduces the possibility of maloperation; And adopt electromagnetic locator as manual auxiliary equipment, coordinate the automatic control of motor, make to move liquid process accurate contraposition, simple to operate, operating efficiency is high.The method that adopts pipettor of the present invention to move liquid is manually assisted with motor and is automatically controlled and combine, and simple to operate, precision is high, and operating efficiency is high.
Accompanying drawing explanation
Fig. 1 is the structural representation of pipettor of the present invention;
Fig. 2 is the partial structurtes schematic diagram of interrupting of pipettor of the present invention;
Fig. 3 is the partial sectional view of interrupting of pipettor of the present invention;
Fig. 4 is the electromagnetic locator of the pipettor of the present invention structural representation in microswitch normally open;
Fig. 5 is the electromagnetic locator of the pipettor of the present invention structural representation in microswitch closure state.
The specific embodiment
Below in conjunction with accompanying drawing, describe mechanism of the present invention and operation principle in detail.For better explanation the present embodiment, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product; To those skilled in the art, in accompanying drawing some known configurations and explanation thereof may to omit be understandable.The corresponding same or analogous parts of same or analogous label.
Refer to shown in Fig. 1-5, the invention provides a kind of pipettor, comprise base 3, be located at base 3 one sides column 2, be located at Z axis motor 21 on column 2, be located at the first screw mandrel 22 of Z axis motor 21 output shafts, the electromagnetic locator 30 of being located at slide block 24 on the first screw mandrel 22, being fixedly installed in the pipetting device 1 on slide block 24 and being located on base 3 and being electrically connected with Z axis motor 21.
Described pipetting device 1 comprises the piston head Connection Bracket 10 of connection sliding block 24, be installed in the piston motor 11 on piston head Connection Bracket 10, the second screw mandrel 111 being connected with the output shaft of piston motor 11, the piston fixed head 12 being connected with the second screw mandrel 111, be installed in the several pistons 14 on piston fixed head 12, be set in the piston/cylinder 16 on piston 14, the upper end of piston/cylinder 16 is provided with sealing ring 13, the corresponding piston in the lower end of piston/cylinder 16 is provided with severally interrupts 15, described 15 upper ends of interrupting are for packed-piston cylinder body 16, described 15 lower ends of interrupting are for being socketed suction nozzle 17.Described piston 14, sealing ring 13, piston/cylinder 16, and interrupt and between 15, form seal cavity 141, described piston 14 moves up and down the interior generation pressure of seal cavity 141 is changed in seal cavity 141, thereby can promote liquid, enters or discharges and interrupt in the suction nozzle 17 of 15 belows sockets.When the second screw mandrel 111 moves upward, seal cavity 141 internal pressures reduce, and liquid enters in suction nozzle 17 from the aperture of suction nozzle 17; When the second screw mandrel 111 moves downward, seal cavity 141 internal pressures increase, and liquid is discharged from from the aperture of suction nozzle 17.
When described Z axis motor 21 rotates, can drive the first screw mandrel 22 to rotate, slide block 24 moves up and down under the huge torsional forces effect of Z axis motor 21 and the first screw mandrel 22, thereby drives pipetting device 1 to move up and down, the 15 insertion suction nozzles 17 of interrupting also compress, and realize the function of Auto-mounting suction nozzle 17.
Air tight for guaranteeing to plug suction nozzle 17, described in 15 lower ends of interrupting be respectively equipped with the directed bulge loop 152 that is positioned at the sealing bulge loop 151 of below and is positioned at top.Described interrupt 15 and sealing bulge loop 151 provided thereon and directed bulge loop 152 be formed in one and make, easy to process.The described 15 employing flexible materials of interrupting are made, and have certain amount of elastic deformation, are beneficial to install suction nozzle 17, and sealing effectiveness is better.Described sealing bulge loop 151 and directed bulge loop 152 are all the arc bulge loops of being located at 15 outsides of interrupting.The contact area of itself and suction nozzle 17 is less, be easy to deeply insert in suction nozzle 17 be beneficial to installation, and sealing effectiveness is better.Described sealing bulge loop 151 arranging of height have larger impact to sealing effectiveness, if the excessive height of sealing bulge loop 151, bulge loop structure is not wear-resisting; If the height of sealing bulge loop 151 is too small, the accuracy of processing is difficult to guarantee.Preferably, relatively interrupt 15 height of described sealing bulge loop 151 is 0.2mm ~ 0.212mm, is not only beneficial to processing but also can guarantees good sealing effectiveness.
Described directed bulge loop 152 is main to be given to interrupt 15 provides guide effect allow suction nozzle 17 direction accurate positionings, possesses certain sealing effectiveness simultaneously.Longitudinal contact length that described directed bulge loop 152 has increased suction nozzle 17 and interrupted between 15, the angle that suction nozzle 17 is swung is restricted, thereby gives suction nozzle 17 orientations to guarantee suction nozzle 17 straight down.Relatively interrupt 15 height of described sealing bulge loop 152 is 0.18mm ~ 0.192mm, has good orientation and sealing function.For coordinating the installation of suction nozzle 17, the suction nozzle box below of depositing suction nozzle 17 adopts flexible material, makes to install suction nozzle 17 in suction nozzle 17 processes and can, because being subject to adjustment that force direction makes certain angle better to fit and to interrupt 15, complete sealing.When suction nozzle 17 is installed, the sealing bulge loop 151 of interrupting on 15 utilizes its arc design to insert in suction nozzle 17, then rely on the guide effect of directed bulge loop 152, and coordinate the reverse extrusion of suction nozzle box, to interrupt and 15 insert securely in suction nozzle 17, because the frictional resistance between sealing bulge loop 151 and directed bulge loop 152 and suction nozzle 17 inwalls makes mounted suction nozzle 17, can be uneasily from interrupting, 15 not fall down.
On described piston fixed head 12, be provided with push rod 182, the lower end of push rod 182 is provided with a desorption push pedal 181, this desorption push pedal 181 is arranged at piston/cylinder 16 belows, and the top that is arranged at 15 the directed bulge loop 152 of interrupting, this desorption push pedal 181 can move up and down between piston/cylinder 16 lower ends and the upper end of directed bulge loop 152.During the 11 forward running of piston motor, drive the second screw mandrel 111 to rotate, the torsional forces of its generation drives piston fixed head 12 to move down, described piston 14, push rod 182, and suction nozzle push pedal 181 moves down under the effect of piston fixed head 12, when suction nozzle push pedal 181 moves to the position that can contact suction nozzle 17 upper ends, be defined as piston 14 and be positioned at zero graduation line position, control piston motor 11 continues forward running, by being moved downward, piston fixed head 12 promotes a downward thrust of push rod 182 181 pairs of suction nozzles of a drive desorption push pedal, 17 generations downwards, suction nozzle 17 is departed from and interrupt 15.
Further, described column 2 is provided with the polished rod 23 parallel with the first screw mandrel 22, and on described slide block 24, corresponding polished rod 23 is provided with through hole 241, and described polished rod 23 is located in this through hole 241, thereby guarantees that pipetting device 1 moving up and down on column 2 is more stable.
Described pipettor also comprises touch screen control panel (not shown) and the operating grip (not shown) that is electrically connected described Z axis motor 21, described piston motor 11 and described electromagnetic locator 30, in order to acknowledge(ment) signal and input of control commands, thereby control respectively Z axis motor 21, piston motor 11 and electromagnetic locator 30.
Described electromagnetic locator 30 comprises the guide rail 31 that is installed on base 3, be located at plate rail 32 on guide rail 31, be located at guide rail 31 relative both sides locating piece 33, be located in plate rail 32 and be positioned at guide rail 31 tops the first installation base 321, be located at electromagnet 322 that plate rail 32 and relative positioning piece 33 arrange, be located on locating piece 33 and the iron block 331 of electromagnet 322 settings relatively.Described electromagnet 322 is located on this first installation base 321, between described electromagnet 322 and the first installation base 321, is provided with spring 323, has certain cushioning effect.Described electromagnet 322 is electrically connected described Z axis motor 21.Described plate rail 32 is for placing the liquid-transfering devices such as suction nozzle box, source solution disk and/or target disc, according to the needs of moving liquid process, select, described plate rail 32 can move horizontally on guide rail 31, with the liquid-transfering device of guaranteeing to be positioned on plate rail 32, moves to suitable position.Described source solution disk and target disc all can be microwell plate, comprise the forms such as 96 holes and 384 holes.Accordingly, preferred described piston 14 is 96, described in to interrupt 15 be also 96, be convenient to the disposable liquid that moves that completes 96 holes, and the liquid that moves in 384 holes only needs to carry out four operations, very convenient.Described electromagnetic locator 30 also comprises the second installation base 324, the crashproof rubber cushion 325 of being located at plate rail 32 being located in plate rail 32 and being positioned at guide rail 31 belows, be located at the microswitch 326 of plate rail 32 and be located on described locating piece 33 and set screw 332 that corresponding described microswitch 326 arranges.Described crashproof rubber cushion 325 and described microswitch 326 are all fixedly installed on described the second installation base 324.When microswitch 326 contacts with set screw 332, microswitch 326 is in closure state; When microswitch 326 does not contact with set screw 332, microswitch 326 is in normally open.
During installation, be positioned at the distance D 1 that the described set screw 332 of the same side and the distance D 3 of microswitch 326 are less than described electromagnet 322 and described iron block 331, the distance D 1 of described electromagnet 322 and described iron block 331 is less than the distance D 2 of described crashproof rubber cushion 325 and described locating piece 33.Preferably, D3 is than the little about 0.2mm of D1, and D1 is than the little about 0.5mm of D2.When hand-driven moving Moving plate frame 32 arrives the locating piece 33 of guide rail 31 1 sides, set screw 332 contacts at first with microswitch 326, because microswitch 326 has certain stroke, thereby can not be damaged, then electromagnet 322 is with iron block 33 contacts, and now electromagnet 322 is because the cushioning effect of spring 323 can certain amplitude retreat, last crashproof rubber cushion 325 is with locating piece 33 contacts, because the elasticity of crashproof rubber cushion 325 can prevent overshoot, and stop motion, now, plate rail 32 arrives precalculated position, be positioned over liquid-transfering device on plate rail 32 can with 15 accurate contrapositions of interrupting, microswitch 326 contacts with set screw 332, microswitch 326 is in closure state, microswitch 326 sends the signal moving into place, software moves permission Z axis motor 21, open 21 runnings of Z axis motor, drive the first screw mandrel 22 to rotate, make to move under slide block 24 in the vertical directions, thereby drive pipetting device 1 to move up and down.In Z axis motor 21 operation process, electromagnet 322 energising produces powerful magnetic force, guarantees that plate rail 32 is fixed to abut on the locating piece 33 of guide rail 31 1 sides (referring to Fig. 5), avoids now External Force Acting skew plate rail 32 and causes moving liquid to bit-errors.When needs move plate rail 32, the first power-off of electromagnet 322, then operator can move with hand propelled plate rail 32.When plate rail 32 leaves the locating piece 33 at guide rail 31 two ends, microswitch 326 has departed from the compressing of set screw 332 and again in open mode.Now illustrate that plate rail 32 is not on correct position, microswitch 326 can be used as in open mode the signal that a movement does not put in place, and software does not allow Z axis motor 21 to turn round by not being performed and prompting operation person need to move correct position.
In sum, pipettor of the present invention, by a Z axis motor is set, is connected with Z axis motor pipetting device by the first screw mandrel, and pipetting device can be realized Auto-mounting suction nozzle under the drive of Z axis motor, easy to operate succinct, installs firmly, and workload is little; Pipettor of the present invention, by a desorption push pedal is set, can be realized desorption head automatically, greatly reduces experimenter's workload; Adopting 96 pistons to move liquid can efficiently and reliably work, and without operator, pays very large muscle power, greatly reduces the possibility of maloperation; And adopt electromagnetic locator as manual auxiliary equipment, coordinate the automatic control of motor, make to move liquid process accurate contraposition, simple to operate, operating efficiency is high.
In addition, the present invention also provides a kind of method of using above-mentioned pipettor to move liquid, comprises the following steps:
Manually control mobile plate rail, suction nozzle box on plate rail is positioned under interrupting of pipettor, control Z axis electric machine rotation, band movable slider moves downward, the pipetting device integral body that makes to be fixedly installed on slide block moves downward near suction nozzle box, to the contact suction nozzle of interrupting, slows down and continues downwards, the malleation that makes to interrupt is inserted in suction nozzle, until interrupt, cannot move downward.Control Z axis motor antiport, upwards mention pipetting device, complete thus the installation of suction nozzle.This pipettor can disposablely complete the installation of 96 suction nozzles, and adopts mechanical control method, and pressure downward during installation is even, and easy for installation being easy to of whole process controlled, and suction nozzle good sealing effect.
Step 4, absorption source solution.
When Z axis motor shuts down, electromagnet is in off-position, direct hand-driven moving Moving plate frame, the source solution disk of making is positioned under suction nozzle, control the running of Z axis motor forward, make suction nozzle position arrive the suction range H2 setting, then control piston motor positive movement sucks liquid to suction nozzle, then by software, automatically control the counter motion of Z axis motor and mention to moving liquid height H 1, complete the sucking action of source solution.
Step 5, source solution is discharged in target disc.
Hand-driven moving Moving plate frame again, target disc is positioned under suction nozzle, control the running of Z axis motor forward, make suction nozzle position arrive the tapping height H 3 of setting, then the counter motion of control piston motor is expelled in target disc the liquid in suction nozzle, then by software, automatically controlling the counter motion of Z axis motor mentions to moving liquid height H 1, completing and moves liquid.
Step 6, dismounting suction nozzle.
Control piston motor continues counter motion, drives piston to move downward to extreme position, discharges air.Hand-driven moving Moving plate frame, make suction nozzle box be positioned at suction nozzle under.Control piston fixed head moves downward promotion push rod and drives a desorption push pedal to produce a downward thrust to suction nozzle downwards, makes suction nozzle depart to interrupt and falls in suction nozzle box, completes thus the dismounting of suction nozzle.
The method that adopts pipettor of the present invention to move liquid is manually assisted with motor and is automatically controlled and combine, and simple to operate, precision is high, and operating efficiency is high.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without also giving all embodiments.All any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in the protection domain of the claims in the present invention.
Claims (10)
1. a pipettor, it is characterized in that, comprise base, be located at base one side column, be located at Z axis motor on column, be located at the first screw mandrel of Z axis motor output shaft, the electromagnetic locator of being located at slide block on the first screw mandrel, being fixedly installed in the pipetting device on slide block and being located on base and being electrically connected with Z axis motor, described pipetting device comprises the piston head Connection Bracket of connection sliding block, be installed in the piston motor on piston head Connection Bracket, the second screw mandrel being connected with the output shaft of piston motor, the piston fixed head being connected with the second screw mandrel, be installed in the several pistons on piston fixed head, be set in the piston/cylinder on piston, the corresponding piston in lower end of piston/cylinder is provided with several interrupting, the described upper end of interrupting is for packed-piston cylinder body, the described lower end of interrupting is for being socketed suction nozzle, the described lower end of interrupting is respectively equipped with the directed bulge loop that is positioned at the sealing bulge loop of below and is positioned at top, on described piston fixed head, be provided with push rod, the lower end of push rod is provided with a desorption push pedal, this desorption push pedal is arranged at piston/cylinder below, and be arranged at the top of the directed bulge loop of interrupting, described electromagnetic locator is provided with plate rail.
2. pipettor as claimed in claim 1, it is characterized in that, the upper end of described piston/cylinder is provided with sealing ring, described piston, sealing ring, piston/cylinder, and interrupt between form seal cavity, described piston moves up and down and makes to produce in seal cavity pressure and change in seal cavity, thereby can promote liquid, enters or discharges and interrupt in the suction nozzle of below socket.
3. pipettor as claimed in claim 1, is characterized in that, described in interrupt and adopt flexible material to make.
4. pipettor as claimed in claim 1, is characterized in that, also comprises the touch screen control panel and the operating grip that are electrically connected described Z axis motor, described piston motor and described electromagnetic locator.
5. pipettor as claimed in claim 1, is characterized in that, described piston is 96, described in to interrupt be 96.
6. pipettor as claimed in claim 1, is characterized in that, described column is provided with the polished rod parallel with the first screw mandrel, and on described slide block, corresponding polished rod is provided with through hole, and described polished rod is located in this through hole.
7. pipettor as claimed in claim 1, it is characterized in that, described electromagnetic locator comprises the guide rail that is installed on base, the locating piece being located at plate rail on guide rail, being located at the relative both sides of guide rail of sliding, be located in plate rail and the electromagnet of relative positioning piece setting, be located on locating piece and the iron block of electromagnet setting relatively, be located at crashproof rubber cushion in plate rail, be located at the microswitch in plate rail and be located on described locating piece and set screw that corresponding described microswitch arranges, and described electromagnet is electrically connected described Z axis motor; Described electromagnetic locator also comprises to be located in plate rail and to be positioned at the first installation base of guide rail top and to be located at plate rail and to be positioned at the second installation base below guide rail, described electromagnet is located on this first installation base, between this electromagnet and the first installation base, be provided with spring, described crashproof rubber cushion and described microswitch are all fixedly installed on this second installation base.
8. pipettor as claimed in claim 7, it is characterized in that, be positioned at the distance D 1 that the described set screw of the same side and the distance D 3 of microswitch are less than described electromagnet and described iron block, the distance D 1 of described electromagnet and described iron block is less than the distance D 2 of described crashproof rubber cushion and described locating piece.
9. the method that the pipettor of use as described in any one in claim 1 ~ 8 moves liquid, is characterized in that, comprises the following steps:
Step 1, pipettor is provided, suction nozzle box, source solution disk, the target disc of suction nozzle are housed, respectively suction nozzle box, source solution disk, target disc are placed on the plate rail of pipettor successively; Described suction nozzle box below adopts flexible material;
Step 2, according to source solution disk, target disc position, set respectively and move liquid height H 1, suction range H2, tapping height H 3;
Step 3, hand-driven moving Moving plate frame, suction nozzle box on plate rail is positioned under interrupting of pipettor, control Z axis electric machine rotation, band movable slider moves downward, the pipetting device integral body that makes to be fixedly installed on slide block moves downward near suction nozzle box, to the contact suction nozzle of interrupting, slows down and continues downwards, the malleation that makes to interrupt is inserted in suction nozzle, until interrupt, cannot move downward; Control Z axis motor antiport, upwards mention pipetting device, complete thus the installation of suction nozzle;
Step 4, hand-driven moving Moving plate frame, the source solution disk of making is positioned under suction nozzle, control the running of Z axis motor forward, make suction nozzle position arrive the suction range H2 setting, then control piston motor positive movement sucks liquid to suction nozzle, then by software, automatically control the counter motion of Z axis motor and mention to moving liquid height H 1, complete the sucking action of source solution;
Step 5, hand-driven moving Moving plate frame again, target disc is positioned under suction nozzle, control the running of Z axis motor forward, make suction nozzle position arrive the tapping height H 3 of setting, then the counter motion of control piston motor is expelled in target disc the liquid in suction nozzle, then by software, automatically controlling the counter motion of Z axis motor mentions to moving liquid height H 1, completing and moves liquid.
10. method of moving liquid as claimed in claim 9, is characterized in that, also comprises that step 6, control piston motor continue counter motion, drives piston to move downward, and discharges air; Hand-driven moving Moving plate frame, make suction nozzle box be positioned at suction nozzle under, control piston fixed head moves downward and promotes push rod and drive a desorption push pedal to produce a downward thrust to suction nozzle downwards, makes suction nozzle depart to interrupt and falls in suction nozzle box, completes thus the dismounting of suction nozzle.
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| CN201410004737.0A CN103691498B (en) | 2014-01-06 | 2014-01-06 | Liquid-moving machine and method of moving liquid by same |
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| CN201410004737.0A CN103691498B (en) | 2014-01-06 | 2014-01-06 | Liquid-moving machine and method of moving liquid by same |
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| CN103990504A (en) * | 2014-05-27 | 2014-08-20 | 广州冠科生物科技有限公司 | Full-automatic liquid transfer device |
| CN104001563A (en) * | 2014-06-09 | 2014-08-27 | 上海优爱宝机器人技术有限公司 | Pipettor |
| CN104353510A (en) * | 2014-10-27 | 2015-02-18 | 王权 | Liquid transferring device capable of automatically mounting and stacking box-packaged suckers |
| CN105689326A (en) * | 2016-02-03 | 2016-06-22 | 中国原子能科学研究院 | Cleaning liquid recovery device |
| CN106111224A (en) * | 2016-08-02 | 2016-11-16 | 苏州金锐启智能装备科技有限公司 | 96 passage automatic pipettors and assemble method thereof and method of work |
| CN106459866A (en) * | 2014-06-17 | 2017-02-22 | 雅马哈发动机株式会社 | Additional information on a caller |
| CN106582907A (en) * | 2015-10-15 | 2017-04-26 | 常州福生生物技术有限公司 | Pipettor with automatic suction head withdrawal function |
| CN106955752A (en) * | 2017-04-25 | 2017-07-18 | 波顿（上海）生物技术有限公司 | A kind of perforated plate sampling height adjustor |
| CN107858286A (en) * | 2017-12-13 | 2018-03-30 | 山西医科大学 | A kind of vacuum extractor liquid-transfering device |
| CN108043484A (en) * | 2017-12-21 | 2018-05-18 | 江苏集萃智能制造技术研究所有限公司 | A kind of liquid-transfering gun moving mechanism |
| CN108249137A (en) * | 2018-02-01 | 2018-07-06 | 梅木精密工业(珠海)有限公司 | Liquid-transfering sucker arranges the doffer of robot |
| CN108490205A (en) * | 2018-04-10 | 2018-09-04 | 深圳市博辰智控有限公司 | A kind of microwell plate sample adding system and method |
| CN108922342A (en) * | 2018-07-18 | 2018-11-30 | 鲍若妍 | A kind of buoyancy teaching demonstration |
| CN108940394A (en) * | 2017-05-17 | 2018-12-07 | 天津工业大学 | A kind of micropipette mechanism with automatic withdraw of the needle function |
| CN109321429A (en) * | 2018-09-28 | 2019-02-12 | 农业部沼气科学研究所 | A kind of automatical bacterium instrument and microbe to screen method |
| CN110038657A (en) * | 2019-04-11 | 2019-07-23 | 深圳垦拓流体控制有限公司 | It is loaded component and loading device |
| CN110217563A (en) * | 2019-06-11 | 2019-09-10 | 湖南隋侯珠科技有限公司 | A kind of two-in-one pipetting tip arrangement robot |
| CN111060708A (en) * | 2019-12-02 | 2020-04-24 | 江苏圣极基因科技有限公司 | Suction head withdrawing mechanism and liquid transferring system |
| CN111781390A (en) * | 2020-07-27 | 2020-10-16 | 苏州奥美泰克生物技术有限公司 | Four-channel double-circulation synchronous sample transferring system and method |
| CN113976199A (en) * | 2021-11-02 | 2022-01-28 | 美东汇成生命科技(昆山)有限公司 | Liquid-transfering suction head that practicality is high |
| CN114146741A (en) * | 2020-09-08 | 2022-03-08 | 沈馨儿 | Precise liquid transfer device |
| CN115521863A (en) * | 2022-10-31 | 2022-12-27 | 苏州硒瑞恩生物科技有限公司 | Nucleic acid analysis equipment |
| CN116908479A (en) * | 2023-08-07 | 2023-10-20 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Full-automatic hemagglutination and hemagglutination inhibition test pipetting device |
| CN117399090A (en) * | 2023-12-14 | 2024-01-16 | 苏州奥美泰克生物技术有限公司 | High-precision multichannel pipetting gun |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070048188A1 (en) * | 2003-09-26 | 2007-03-01 | Bigus Hans J | Multi-channel pipette device |
| US20110268628A1 (en) * | 2010-05-03 | 2011-11-03 | Integra Biosciences Corp. (Formerly Viaflo Corporation) | Unintended Motion Control For Manually Directed Multi-Channel Electronic Pipettor |
| CN202315928U (en) * | 2011-11-10 | 2012-07-11 | 美德创新（北京）科技有限公司 | Mounting shaft for suction head of pipettor |
| CN102917795A (en) * | 2010-05-03 | 2013-02-06 | 因特格拉生物科学公司 | Manually directed, multi-channel electronic pipetting system |
| CN103163310A (en) * | 2013-03-15 | 2013-06-19 | 东南大学 | Multi-channel micro-volume sampling device |
-
2014
- 2014-01-06 CN CN201410004737.0A patent/CN103691498B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070048188A1 (en) * | 2003-09-26 | 2007-03-01 | Bigus Hans J | Multi-channel pipette device |
| US20110268628A1 (en) * | 2010-05-03 | 2011-11-03 | Integra Biosciences Corp. (Formerly Viaflo Corporation) | Unintended Motion Control For Manually Directed Multi-Channel Electronic Pipettor |
| CN102917795A (en) * | 2010-05-03 | 2013-02-06 | 因特格拉生物科学公司 | Manually directed, multi-channel electronic pipetting system |
| CN202315928U (en) * | 2011-11-10 | 2012-07-11 | 美德创新（北京）科技有限公司 | Mounting shaft for suction head of pipettor |
| CN103163310A (en) * | 2013-03-15 | 2013-06-19 | 东南大学 | Multi-channel micro-volume sampling device |
Non-Patent Citations (1)
| Title |
|---|
| 佛山市德淙科学仪器有限公司: "MEDUSA 96道移液器软件显示及操作说明", 《中国生物器材网》 * |
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| CN108940394A (en) * | 2017-05-17 | 2018-12-07 | 天津工业大学 | A kind of micropipette mechanism with automatic withdraw of the needle function |
| CN107858286A (en) * | 2017-12-13 | 2018-03-30 | 山西医科大学 | A kind of vacuum extractor liquid-transfering device |
| CN108043484A (en) * | 2017-12-21 | 2018-05-18 | 江苏集萃智能制造技术研究所有限公司 | A kind of liquid-transfering gun moving mechanism |
| CN108249137A (en) * | 2018-02-01 | 2018-07-06 | 梅木精密工业(珠海)有限公司 | Liquid-transfering sucker arranges the doffer of robot |
| CN108249137B (en) * | 2018-02-01 | 2024-04-23 | 梅木精密工业(珠海)有限公司 | Discharging device of pipetting suction head arrangement robot |
| CN108490205A (en) * | 2018-04-10 | 2018-09-04 | 深圳市博辰智控有限公司 | A kind of microwell plate sample adding system and method |
| CN108922342A (en) * | 2018-07-18 | 2018-11-30 | 鲍若妍 | A kind of buoyancy teaching demonstration |
| CN109321429B (en) * | 2018-09-28 | 2020-09-08 | 农业部沼气科学研究所 | Automatic bacterium picking instrument and microorganism screening method |
| CN109321429A (en) * | 2018-09-28 | 2019-02-12 | 农业部沼气科学研究所 | A kind of automatical bacterium instrument and microbe to screen method |
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| CN110217563B (en) * | 2019-06-11 | 2024-04-19 | 湖南隋侯珠科技有限公司 | Robot is arranged to two unification pipetting tips |
| CN110217563A (en) * | 2019-06-11 | 2019-09-10 | 湖南隋侯珠科技有限公司 | A kind of two-in-one pipetting tip arrangement robot |
| CN111060708A (en) * | 2019-12-02 | 2020-04-24 | 江苏圣极基因科技有限公司 | Suction head withdrawing mechanism and liquid transferring system |
| CN111060708B (en) * | 2019-12-02 | 2023-10-24 | 江苏圣极基因科技有限公司 | Suction head retreating mechanism and pipetting system |
| CN111781390A (en) * | 2020-07-27 | 2020-10-16 | 苏州奥美泰克生物技术有限公司 | Four-channel double-circulation synchronous sample transferring system and method |
| CN111781390B (en) * | 2020-07-27 | 2023-09-05 | 苏州奥美泰克生物技术有限公司 | Four-channel double-circulation synchronous sample transferring system and method |
| CN114146741A (en) * | 2020-09-08 | 2022-03-08 | 沈馨儿 | Precise liquid transfer device |
| CN113976199A (en) * | 2021-11-02 | 2022-01-28 | 美东汇成生命科技(昆山)有限公司 | Liquid-transfering suction head that practicality is high |
| CN115521863A (en) * | 2022-10-31 | 2022-12-27 | 苏州硒瑞恩生物科技有限公司 | Nucleic acid analysis equipment |
| CN115521863B (en) * | 2022-10-31 | 2023-12-05 | 苏州硒瑞恩生物科技有限公司 | Nucleic acid analysis equipment |
| CN116908479A (en) * | 2023-08-07 | 2023-10-20 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Full-automatic hemagglutination and hemagglutination inhibition test pipetting device |
| CN116908479B (en) * | 2023-08-07 | 2024-04-19 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Full-automatic hemagglutination and hemagglutination inhibition test pipetting device |
| CN117399090B (en) * | 2023-12-14 | 2024-03-01 | 苏州奥美泰克生物技术有限公司 | High-precision multichannel pipetting gun |
| CN117399090A (en) * | 2023-12-14 | 2024-01-16 | 苏州奥美泰克生物技术有限公司 | High-precision multichannel pipetting gun |
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