CN103667089B - Take rural organic wastes as microbial inoculum and the application thereof that fermentation raw material prepares fertilizer - Google Patents
Take rural organic wastes as microbial inoculum and the application thereof that fermentation raw material prepares fertilizer Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses with rural organic wastes is microbial inoculum and the application thereof that fermentation raw material prepares fertilizer.Microbial inoculum for the preparation of fertilizer provided by the present invention, its activeconstituents is made up of viride and yeast saccharomyces cerevisiae.Microbial inoculum for the preparation of fertilizer of the present invention is that fertilizer prepared by fermentation raw material all has significant effect of increasing production to cucumber and green pepper with rural organic wastes, and cucumber production promoting can be made to be 21.98%, and green pepper can be made to increase production 19.71%.The microbial inoculum for the preparation of fertilizer of the present invention is utilized to be that to prepare fertilizer be the effective way of rural organic wastes being carried out to recycling to fermentation raw material with rural organic wastes, and this zymotechnique is simple and easy to do, be very suitable for applying in Rural areas.
Description
Technical field
The present invention relates to rural organic wastes is microbial inoculum and the application thereof that fermentation raw material prepares fertilizer.
Background technology
Current due to dealing with improperly rural organic wastes such as the organic compositions that becomes thoroughly decomposed (the residual body of rubbish from cooking, melon and fruit peel and vegetables) in agricultural crop straw, livestock and poultry cultivation ight soil, rural area solid life refuse, the problem of environmental pollution caused is very serious.How these rural organic wastes are effectively processed, alleviate its impact on environment, become a problem demanding prompt solution.
The rotten fermentation of heap is under certain processing condition, ferments, becomes thoroughly decomposed, degrades, make it to become stable organic matter to organic waste, and utilize the heat produced in fermenting process to kill Biochemical processes that harmful microorganism reaches harmless treatment.The rotten effect of fermenting the organic fertilizer obtained of heap depends on the rotten fermenting agent of adopted heap.
Summary of the invention
It take rural organic wastes as the microbial inoculum that fermentation raw material prepares fertilizer that technical problem to be solved by this invention is to provide a kind of being applicable to.
Microbial inoculum for the preparation of fertilizer provided by the present invention, its activeconstituents is made up of viride and yeast saccharomyces cerevisiae.
Above-mentioned for the preparation of in the microbial inoculum of fertilizer, described viride is numbered ACCC30206 China Committee for Culture Collection of Microorganisms's agricultural microorganism center, and described yeast saccharomyces cerevisiae is numbered ACCC20065 China Committee for Culture Collection of Microorganisms's agricultural microorganism center.
Above-mentioned for the preparation of in the microbial inoculum of fertilizer, colony-forming unit (cfu) the number ratio of described viride and described yeast saccharomyces cerevisiae can be (1-2): (1-2), as 1:1.
The above-mentioned microbial inoculum for the preparation of fertilizer also can comprise carrier.Described carrier can be solid carrier or liquid vehicle.Described solid carrier is mineral material, biomaterial or macromolecular compound; Described mineral material is at least one in clay, talcum, kaolin, montmorillonite, white carbon, zeolite, silica, turfy soil and diatomite; Described biomaterial is at least one in the ight soil of the stalk of all kinds of crop, loose shell, straw, Pericarppium arachidis hypogaeae, Semen Maydis powder, bean powder, starch, the peat composed of rotten mosses and animal; Described macromolecular compound is polyvinyl alcohol and/or polyglycol.Described liquid vehicle can be organic solvent, vegetables oil, mineral oil or water; Described organic solvent is decane and/or dodecane.In described microbial inoculum, described viride and described yeast saccharomyces cerevisiae can exist with the form of the fermented liquid of viable cell, viable cell by cultivating, the filtrate of cell culture or the mixture of cell and filtrate.The formulation of described microbial inoculum can be multiple formulation, as liquor, emulsion, suspension agent, pulvis, granule, wettable powder or water dispersible granules.
The above-mentioned microbial inoculum for the preparation of fertilizer is being that the application that fermentation raw material is prepared in fertilizer also belongs to protection scope of the present invention with rural organic wastes.
In above-mentioned application, described rural organic wastes is the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse.
Organic in the solid life refuse of described rural area becomes thoroughly decomposed into the mixture that can be the residual body of vegetables, melon and fruit peel and rubbish from cooking.
The invention provides a kind of method preparing fertilizer specifically.
The method preparing fertilizer provided by the present invention, comprise and the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse is carried out being mixed to get the rotten fermentation raw material of heap, in the rotten fermentation raw material of described heap, add described microbial inoculum obtain heap body material, described heap body material is made heap body and carry out fermentation and obtain fertilizer.
Above-mentionedly prepare in the method for fertilizer, the mass ratio of the organic composition that becomes thoroughly decomposed in described agricultural crop straw, described livestock and poultry cultivation ight soil and described rural area solid life refuse is 1:2.5:1.5, wherein, the quality of described agricultural crop straw in water content for 15%, the quality of described livestock and poultry cultivation ight soil in water content 30%, the quality of the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area in water content for 45%.
Above-mentionedly prepare in the method for fertilizer, the proportioning of the rotten fermentation raw material of described heap and described microbial inoculum is that to add total thalline content in every kilogram of rotten fermentation raw material of described heap be 5 × 10
9the described microbial inoculum of cfu.
Above-mentionedly prepare in the method for fertilizer, described heap body can be rectangular parallelepiped, and the length of described heap body is 6 meters, and wide is 3 meters, and height can be 1.4 meters-1.6 meters (as 1.5 meters).
Above-mentionedly prepare in the method for fertilizer, described fermentation temperature be 20-35 DEG C (as 30-35 DEG C), relative humidity is that 50%-80%(is as 60%) environment in carry out fermentation 21 days, turning in every 7 days.
Above-mentionedly prepare in the method for fertilizer, organic in the solid life refuse of described rural area becomes thoroughly decomposed into the mixture that can be the residual body of vegetables, melon and fruit peel and rubbish from cooking.
In an embodiment of the invention, described agricultural crop straw is maize straw, described livestock and poultry cultivation ight soil is cow dung, and the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area is mixed according to the mass ratio of 1:2:2 by the residual body of vegetables, melon and fruit peel and rubbish from cooking.Wherein, the residual body of vegetables is Collina and discarded Chinese cabbage leaf, and melon and fruit peel is Watermelon rind, and rubbish from cooking is leftovers and leftovers; The quality of the residual body of vegetables, melon and fruit peel and rubbish from cooking with 45% water content meter.
Present invention also offers a kind of method of raising cultivated plant output specifically.
The method of raising cultivated plant output provided by the present invention, comprises and apply above-mentioned fertilizer in the soil of described cultivated plant, improve the output of described cultivated plant.
In the method for above-mentioned raising cultivated plant output, described raising cultivated plant output can be and improves cucumber yield (cucumber fruits output) or improve yield of hot pepper (pepper fruit output).
In the method for above-mentioned raising cultivated plant output, when cultivated plant be cucumber or capsicum time, the consumption of described fertilizer is the 10 grams of described fertilizers of cultivated plant described in every strain.
In above-mentioned application, the mass ratio of the organic composition that becomes thoroughly decomposed in described agricultural crop straw, described livestock and poultry cultivation ight soil and described rural area solid life refuse can be 1:2.5:1.5, wherein, the quality of described agricultural crop straw in water content for 15%, the quality of described livestock and poultry cultivation ight soil in water content 30%, the quality of the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area in water content for 45%.
In an embodiment of the invention, described agricultural crop straw is maize straw, described livestock and poultry cultivation ight soil is cow dung, and the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area is mixed according to the mass ratio of 1:2:2 by the residual body of vegetables, melon and fruit peel and rubbish from cooking.Wherein, the residual body of vegetables is Collina and discarded Chinese cabbage leaf, and melon and fruit peel is Watermelon rind, and rubbish from cooking is leftovers and leftovers; The quality of the residual body of vegetables, melon and fruit peel and rubbish from cooking with 45% water content meter.
Described raising cultivated plant output can be and improves cucumber yield (cucumber fruits output) or improve yield of hot pepper (pepper fruit output)
Experiment proves, the microbial inoculum for the preparation of fertilizer of the present invention is that fertilizer prepared by fermentation raw material all has significant effect of increasing production to cucumber and green pepper with rural organic wastes, and cucumber production promoting can be made to be 21.98%, and green pepper can be made to increase production 19.71%.The microbial inoculum for the preparation of fertilizer of the present invention is utilized to be that to prepare fertilizer be the effective way of rural organic wastes being carried out to recycling to fermentation raw material with rural organic wastes, and this zymotechnique is simple and easy to do, be very suitable for applying in Rural areas.
Embodiment
Below in conjunction with embodiment, the present invention is further described in detail, the embodiment provided only in order to illustrate the present invention, instead of in order to limit the scope of the invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Viride (Trichodermaviride) ACCC30206 used in following embodiment and yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 was all concealed in China Committee for Culture Collection of Microorganisms's agricultural microorganism center and (is called for short ACCC before the applying date of the application, address: No.12 ,zhongguancun south street,Haidian District, Beijing, INST OF AGRICULTURAL RESOURCES, postcode 100081), viride (Trichodermaviride) ACCC30206 and yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 are July 26 nineteen ninety collection day, from collection, the public can obtain this two bacterial strains from China Committee for Culture Collection of Microorganisms's agricultural microorganism center.
In following embodiment, full nitrogen, full phosphorus, the full nutrient content such as potassium, organic matter of all samples detects according to organic fertilizer standard NY525-2012, and total nutrient content refers to full nitrogen, full phosphorus, full potassium content sum.
Cucumber variety in following embodiment is match storehouse, and green pepper kind is red Susan (Li Shigui, Wang Fei, Gu Jingang, Zhu Changxiong.The application of microbiobacterial agent in agricultural wastes compost maturity process and field test effect thereof.JOURNAL OF MICROBIOLOGY, 2011,31(6): 62-65.), provide by Vegetable & Flower Inst., Chinese Academy of Agriculture Science, the public can obtain from Chinese Academy of Agricultural Sciences's agricultural resource and agricultural regionalization institute, and this biomaterial related experiment of the present invention of only attaching most importance to again is used, not can be used as other purposes and uses.
Embodiment 1, microbial inoculum for the preparation of fertilizer
For the preparation of the microbial inoculum of fertilizer in this application also referred to as organic material composting microbial inoculum, the present embodiment has prepared three kinds of organic material composting microbial inoculums, i.e. organic material composting microbial inoculum A, organic material composting microbial inoculum B and organic material composting microbial inoculum C.Its preparation method is as follows:
Viride (Trichodermaviride) ACCC30206 is seeded in PDA medium slant, yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 is seeded in GPYA medium slant, be placed in 28 DEG C of incubator activation culture 2-3 days, obtain yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 of viride (Trichodermaviride) ACCC30206 and the activation activated.
Viride (Trichodermaviride) ACCC30206 of activation is accessed yeast saccharomyces cerevisiae (Saccharomycescerevisiae) the ACCC20065 access GPY substratum of PD substratum, activation, all at 28 DEG C, 200r/min shaking culture 3 days, obtains viride (Trichodermaviride) ACCC30206 fermented liquid (5 × 10 respectively
9and yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 fermented liquid (5 × 10 cfu/ml)
9cfu/ml).Viride (Trichodermaviride) ACCC30206 fermented liquid and yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 fermented liquid are carried out being mixed to get organic material composting microbial inoculum A according to colony-forming unit (cfu) the number ratio of 1:1.In this organic material composting microbial inoculum A, the content of viride (Trichodermaviride) ACCC30206 is 2.5 × 10
9the content of cfu/ml, yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 is 2.5 × 10
9cfu/ml.Viride (Trichodermaviride) ACCC30206 fermented liquid and yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 fermented liquid are carried out being mixed to get organic material composting microbial inoculum B according to colony-forming unit (cfu) the number ratio of 1:2.In this organic material composting microbial inoculum B, the content of viride (Trichodermaviride) ACCC30206 is 1.7 × 10
9the content of cfu/ml, yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 is 3.3 × 10
9cfu/ml.Viride (Trichodermaviride) ACCC30206 fermented liquid and yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 fermented liquid are carried out being mixed to get organic material composting microbial inoculum C according to colony-forming unit (cfu) the number ratio of 2:1.In this organic material composting microbial inoculum C, the content of viride (Trichodermaviride) ACCC30206 is 3.3 × 10
9the content of cfu/ml, yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 is 1.7 × 10
9cfu/ml.
Wherein, the preparation method of substratum is as follows:
PD substratum: the potato that potato 200g(removes the peel is cut into small pieces, boiled and crossed elimination filtrate in 30 minutes), glucose 20g, be settled to 1000ml with distilled water, 121 DEG C of vapor sterilization 20min.
PDA substratum: the potato that potato 200g(removes the peel is cut into small pieces, boiled and crossed elimination filtrate in 30 minutes), glucose 20g, agar 18-20g, is settled to 1000ml with distilled water, 121 DEG C of vapor sterilization 20min.
GPY substratum: glucose 40g, peptone 5g, yeast extract paste 5g, be settled to 1000ml with distilled water, 121 DEG C of vapor sterilization 20min.
GPYA substratum: glucose 40g, peptone 5g, yeast extract paste 5g, agar 18-20g, be settled to 1000ml with distilled water, 121 DEG C of vapor sterilization 20min.
Embodiment 2, utilizing the organic material composting microbial inoculum of embodiment 1, with the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse for piling rotten fermentation raw material, adopting three forms to pile rotten fermentation for organic fertilizer
1, the preparation of rotten fermentation raw material is piled
Organic composition that becomes thoroughly decomposed in the rural area solid life refuse adopted in this step is mixed according to the mass ratio of 1:2:2 by the residual body of vegetables, melon and fruit peel and rubbish from cooking.Wherein, the residual body of vegetables is Collina and discarded Chinese cabbage leaf, and melon and fruit peel is Watermelon rind, and rubbish from cooking is leftovers and leftovers; The quality of the residual body of vegetables, melon and fruit peel and rubbish from cooking with 45% water content meter.
This step maize straw and organic in the solid life refuse of rural area are become thoroughly decomposed into cut into length be the fritter of 2.0cm after and cow dung be mixed to get the rotten fermentation raw material of heap.The rotten fermentation raw material of this heap is mixed to get according to the mass ratio of 1:2.5:1.5 by the organic composition that becomes thoroughly decomposed in maize straw, cow dung and rural area solid life refuse.
Wherein, the water content of above-mentioned maize straw is 15%, and the water content of cow dung is 30%, and the water content of the organic composition that becomes thoroughly decomposed in the solid life refuse of rural area is 45%.The nutrient content of three kinds of rotten raw materials of heap is as shown in table 1.
The nutrient content (in butt) of table 1, three kinds of rotten raw materials of heap
2, the preparation of body material is piled
In the rotten fermentation raw material of heap of step 1, add machine material decomposing microbial inoculum A, organic material composting microbial inoculum B and organic material composting microbial inoculum C respectively make heap body material A, heap body material B and heap body material C, concrete method is as follows:
The preparation of 2.1 heap body material A
Adding total thalline content according to every kilogram of rotten fermentation raw material of heap is 5 × 10
9the thalline content of cfu(and viride (Trichodermaviride) ACCC30206 and the thalline content sum of yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 are 5 × 10
9the proportioning of the organic material composting microbial inoculum A of embodiment 1 cfu), the organic material composting microbial inoculum A adding embodiment 1 in the rotten fermentation raw material of heap of step 1 obtains heap body material A.
The preparation of 2.2 heap body material B
Adding total thalline content according to every kilogram of rotten fermentation raw material of heap is 5 × 10
9the thalline content of cfu(and viride (Trichodermaviride) ACCC30206 and the thalline content sum of yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 are 5 × 10
9the proportioning of the organic material composting microbial inoculum B of embodiment 1 cfu), the organic material composting microbial inoculum B adding embodiment 1 in the rotten fermentation raw material of heap of step 1 obtains heap body material B.
The preparation of 2.3 heap body material C
Adding total thalline content according to every kilogram of rotten fermentation raw material of heap is 5 × 10
9the thalline content of cfu(and viride (Trichodermaviride) ACCC30206 and the thalline content sum of yeast saccharomyces cerevisiae (Saccharomycescerevisiae) ACCC20065 are 5 × 10
9the proportioning of the organic material composting microbial inoculum C of embodiment 1 cfu), the organic material composting microbial inoculum C adding embodiment 1 in the rotten fermentation raw material of heap of step 1 obtains heap body material C.
3, heap body material is made heap body to carry out the rotten fermentation of three forms heaps and obtain organic fertilizer
The rotten fermentation of heap is carried out in fermentation cabin, this fermentation cabin comprises Cang Ding, at the bottom of bulkhead and storehouse, perpendicular to arranging multiple lattice wall at the bottom of storehouse at the bottom of storehouse, fermentation cabin lattice wall is divided into 9 lattice by volume, namely described fermentation cabin is divided into 3 three lattice groups by lattice wall, each three lattice groups are made up of adjacent three lattice, the ventilation slot of twice length × wide × height=6m × 0.15m × 0.13m is longitudinally evenly distributed under the storehouse of every lattice, per pass ventilation slot is laid stainless-steel sheet with holes a piece, length × wide × thick=6m × 0.19m × 0.003m of every block stainless-steel sheet, it is that the ventilating pit of 0.012m is beneficial to ventilated that every block stainless-steel sheet is evenly distributed 400 diameters, after stainless-steel sheet completed by ventilation slot, steel plate top is just concordant with at the bottom of storehouse.Three adjacent lattice are three lattice groups, and this fermentation cabin is divided into 3 three lattice groups, are respectively three lattice group A, three lattice group B and three lattice group C.
Prepare organic fertilizer A, organic fertilizer B and organic fertilizer C by the following method simultaneously.
The preparation of 3.1 organic fertilizer A
Add water in the heap body material A of 2.1 preparations and the water content of heap body material A is all adjusted to 60%, heap body material A is made the heap body of length × wide × height=6m × 3m × 1.5m at first lattice of three lattice group A, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain first time fermentation heap body material A, the second lattice first time fermentation heap body material A overturning being entered three lattice group A make the heap body of length × wide × height=6m × 3m × 1.5m, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain second time fermentation heap body material A, the 3rd lattice overturning of second time fermentation heap body material A being entered three lattice group A make the heap body of length × wide × height=6m × 3m × 1.5m, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain organic fertilizer A.
The preparation of 3.2 organic fertilizer B
Replace with except B except by A, all the other are completely with 3.1.Concrete grammar is as follows: adding water in the heap body material B of 2.2 preparations is all adjusted to 60% by the water content of heap body material B, heap body material B is made the heap body of length × wide × height=6m × 3m × 1.5m at first lattice of three lattice group B, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain first time fermentation heap body material B, the second lattice first time fermentation heap body material B overturning being entered three lattice group B make the heap body of length × wide × height=6m × 3m × 1.5m, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain second time fermentation heap body material B, the 3rd lattice second time fermentation heap body material B overturning being entered three lattice group B make the heap body of length × wide × height=6m × 3m × 1.5m, be 30-35 DEG C in temperature, relative humidity is that in the environment of 60%, standing for fermentation obtains organic fertilizer B in 7 days.
The preparation of 3.3 organic fertilizer C
Replace with except C except by A, all the other are completely with 3.1.Concrete grammar is as follows: adding water in the heap body material C of 2.3 preparations is all adjusted to 60% by the water content of heap body material C, heap body material C is made the heap body of length × wide × height=6m × 3m × 1.5m at first lattice of three lattice group C, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain first time fermentation heap body material C, the second lattice first time fermentation heap body material C overturning being entered three lattice group C make the heap body of length × wide × height=6m × 3m × 1.5m, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain second time fermentation heap body material C, the 3rd lattice second time fermentation heap body material C overturning being entered three lattice group C make the heap body of length × wide × height=6m × 3m × 1.5m, be 30-35 DEG C in temperature, relative humidity is standing for fermentation 7 days in the environment of 60%, obtain organic fertilizer C.
4, results and analysis
The nutrient content of sample before and after 4.1 heap corruption fermentations
As shown in table 2, organic fertilizer A is compared with heap body material A, and content of tatal phosphorus significantly increases, and amplification is 6.79%; And organic, full nitrogen and full potassium content decrease, the range of decrease is respectively 6.26%, 4.35% and 5.23%; But total nutrient content then increases to some extent.Organic fertilizer B is compared with heap body material B, and content of tatal phosphorus has increased slightly; And organic, full nitrogen and full potassium content decrease, the range of decrease is respectively 8.23%, 9.52% and 8.51%; Total nutrient content declines 3.39%.Organic fertilizer C is compared with heap body material C, and content of tatal phosphorus slightly increases; And organic, full nitrogen and full potassium content decrease, the range of decrease is respectively 9.81%, 9.52% and 9.29%; Total nutrient content declines 4.42%.
The nutrient content (in butt) of sample before and after the rotten fermentation of table 2, heap
| Sample | Organic % | Full nitrogen (N) % | Full phosphorus (P) % | Full potassium (K) % | Total nutrient % |
| Heap body material A | 52.6 | 1.84 | 3.24 | 1.53 | 6.61 |
| Organic fertilizer A | 49.5 | 1.76 | 3.46 | 1.45 | 6.67 |
| Heap body material B | 52.6 | 1.84 | 3.24 | 1.53 | 6.61 |
| Organic fertilizer B | 48.6 | 1.69 | 3.26 | 1.41 | 6.36 |
| Heap body material C | 52.6 | 1.84 | 3.24 | 1.53 | 6.61 |
| Organic fertilizer C | 47.9 | 1.68 | 3.25 | 1.40 | 6.33 |
The field fertilizer efficiency experiment of 4.2 organic fertilizer
Field test is carried out at Chinese Academy of Agricultural Sciences's Langfang Experimental Base, and the cucumber variety of cultivation is match storehouse, and green pepper kind is red Susan.
Cucumber cultivation experiment is identical with the experimental design method of green pepper cultivation experiments, all as follows: experiment adopts randomized block design, 3 iterons are set, each iteron arranges 4 communities at random, is respectively CK treatment zone, organic fertilizer A treatment zone, organic fertilizer B treatment zone, organic fertilizer C treatment zone.The area of each community is 30m2.
Except spreading manuer in holes except the kind difference of fertilizer in soil, other field management of each process is all identical.Contrast organic fertilizer is the rotten fermentation raw material of the heap step 1 of embodiment 1 prepared is 30-35 DEG C in temperature, relative humidity is leave standstill spontaneous fermentation turning after 7 days in the environment of 60%, be 30-35 DEG C in temperature again, relative humidity is leave standstill spontaneous fermentation turning after 7 days in the environment of 60%, be 30-35 DEG C in temperature again, relative humidity is leave standstill the organic fertilizer that spontaneous fermentation obtains after 7 days in the environment of 60%.In each community respectively when transplanting, earthing field planting again after fertilizer 10 grams of spreading manuer in holes around every vegetable seedling rhizosphere.Wherein, CK treatment zone is contrast, and the fertilizer executed is contrast organic fertilizer; The fertilizer that organic fertilizer A executes treatment zone is organic fertilizer A; The fertilizer that organic fertilizer B executes treatment zone is organic fertilizer B; The fertilizer that organic fertilizer C executes treatment zone is organic fertilizer C.Investigate the output (abbreviation cucumber yield) of cucumber fruits in the harvesting time of cucumber, investigate the output (abbreviation yield of hot pepper) of pepper fruit in the harvesting time of capsicum.Significance of difference analysis is carried out with t-Test.
Result shows that organic fertilizer A, organic fertilizer B and organic fertilizer C are to the obvious effect of increasing production of cucumber and green pepper, and organic fertilizer A is 21.98%(table 3 to the effect of increasing production of cucumber compared with contrast organic fertilizer), be 19.71%(table 4 to the effect of increasing production of green pepper); Organic fertilizer B is 16.57%(table 3 to the effect of increasing production of cucumber), be 14.31%(table 4 to the effect of increasing production of green pepper); Organic fertilizer C is 15.88%(table 3 to the effect of increasing production of cucumber), be 13.67%(table 4 to the effect of increasing production of green pepper).Organic fertilizer A to the obvious effect of increasing production of cucumber and green pepper higher than organic fertilizer B and organic fertilizer C, organic fertilizer B and organic fertilizer C to the effect of increasing production of cucumber and green pepper without significant difference.
Effect of increasing production to cucumber after the rotten fermentation of table 3, countryside wastes heap
| Process | Cell production (kg/ community) | Effect of increasing production (%) | The significance of difference |
| Organic fertilizer A | 106.0 | 21.98 | C |
| Organic fertilizer B | 101.3 | 16.57 | B |
| Organic fertilizer C | 100.7 | 15.88 | B |
| CK | 86.9 | / | A |
Note: in table, data are mean value, between the process that in the significance of difference, letter is different, there were significant differences (P < 0.05), without significant difference (P > 0.05) between the process that in the significance of difference, letter is identical.
After the rotten fermentation of table 4, countryside wastes heap, sample is to the effect of increasing production of green pepper
| Process | Cell production (kg/ community) | Effect of increasing production (%) | The significance of difference |
| Organic fertilizer A | 75.3 | 19.71 | C |
| Organic fertilizer B | 71.9 | 14.31 | B |
| Organic fertilizer C | 71.5 | 13.67 | B |
| CK | 62.9 | / | A |
Note: in table, data are mean value, between the process that in the significance of difference, letter is different, there were significant differences (P < 0.05), without significant difference (P > 0.05) between the process that in the significance of difference, letter is identical.
Claims (8)
1., for the preparation of the microbial inoculum of fertilizer, its activeconstituents is made up of viride and yeast saccharomyces cerevisiae; Described viride is numbered ACCC30206 China Committee for Culture Collection of Microorganisms's agricultural microorganism center, and described yeast saccharomyces cerevisiae is numbered ACCC20065 China Committee for Culture Collection of Microorganisms's agricultural microorganism center.
2. microbial inoculum according to claim 1, is characterized in that: the number of colony forming units of described viride and described yeast saccharomyces cerevisiae is than being (1-2): (1-2).
3. microbial inoculum according to claim 1 and 2, is characterized in that: the number of colony forming units of described viride and described yeast saccharomyces cerevisiae is than being 1:1.
4. in claims 1 to 3, arbitrary described microbial inoculum is taking rural organic wastes as the application that fermentation raw material prepares in fertilizer; Described rural organic wastes is the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse.
5. prepare the method for fertilizer, comprise and the organic composition that becomes thoroughly decomposed in agricultural crop straw, livestock and poultry cultivation ight soil and rural area solid life refuse is carried out being mixed to get the rotten fermentation raw material of heap, in the rotten fermentation raw material of described heap, add arbitrary described microbial inoculum in claims 1 to 3 obtain heap body material, described heap body material is made heap body and carry out fermentation and obtain fertilizer.
6. method according to claim 5, it is characterized in that: the mass ratio of the organic composition that becomes thoroughly decomposed in described agricultural crop straw, described livestock and poultry cultivation ight soil and described rural area solid life refuse is 1:2.5:1.5, wherein, the quality of described agricultural crop straw in water content for 15%, the quality of described livestock and poultry cultivation ight soil in water content 30%, the quality of the organic composition that becomes thoroughly decomposed in the solid life refuse of described rural area in water content for 45%.
7. the method according to claim 5 or 6, is characterized in that: the proportioning of the rotten fermentation raw material of described heap and described microbial inoculum is that to add total thalline content in every kilogram of rotten fermentation raw material of described heap be 5 × 10
9the described microbial inoculum of cfu.
8. improve the method for cultivated plant output, comprise and apply the fertilizer that in claim 5-7 prepared by arbitrary described method in the soil of described cultivated plant, improve the output of described cultivated plant.
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| CN201310652256.6A CN103667089B (en) | 2013-12-05 | 2013-12-05 | Take rural organic wastes as microbial inoculum and the application thereof that fermentation raw material prepares fertilizer |
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| CN106748544B (en) * | 2017-02-13 | 2020-08-18 | 广西壮族自治区农业科学院蔬菜研究所 | Method for preparing enzyme organic fertilizer from rural waste |
| CN107698120B (en) * | 2017-08-01 | 2020-10-16 | 郭云征 | Animal-derived organic matter harmless treatment process, system and application thereof |
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| CN103274774A (en) * | 2013-05-22 | 2013-09-04 | 河海大学 | High-efficiency biological liquid fertilizer taking livestock liquid dung as raw material and preparation method of fertilizer |
| CN103342609A (en) * | 2013-07-17 | 2013-10-09 | 成都华隆生物科技有限公司 | Straw thoroughly-decomposing microbial inoculum capable of being preserved for long time under normal temperature condition |
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| CN103274774A (en) * | 2013-05-22 | 2013-09-04 | 河海大学 | High-efficiency biological liquid fertilizer taking livestock liquid dung as raw material and preparation method of fertilizer |
| CN103342609A (en) * | 2013-07-17 | 2013-10-09 | 成都华隆生物科技有限公司 | Straw thoroughly-decomposing microbial inoculum capable of being preserved for long time under normal temperature condition |
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