CN103651396A - Sterilization composition - Google Patents
Sterilization composition Download PDFInfo
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- CN103651396A CN103651396A CN201310701369.0A CN201310701369A CN103651396A CN 103651396 A CN103651396 A CN 103651396A CN 201310701369 A CN201310701369 A CN 201310701369A CN 103651396 A CN103651396 A CN 103651396A
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- jiean
- peptide
- azoles alcohol
- suspending agent
- sharp eyespot
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Abstract
The invention belongs to the technical field of pesticides and relates to sterilization composition. The sterilization composition is characterized in that active ingredients comprise jiean-peptide and hexaconazole. The composition can be prepared into a suspending agent and a water dispersible granule, is used for controlling wheat sharp eyespot, and is obvious in synergism and safe and free of phytotoxicity to crops.
Description
Technical field the invention belongs to technical field of pesticide, contains specifically the bactericidal composition with synergistic effect of Jiean-peptide and own azoles alcohol.
Background technology
Jiean-peptide (jiean-peptide) derives from the metabolite of bacillus, and the pathogens such as verticillium dahliae, cotton-wilt fusarium, fusarium graminearum, rhizoctonia cerealis, powdery mildew, eliminating vegetable botrytis and downy mildew, muskmelon phytophthora root rot bacterium are all had to stronger inhibition growth and control efficiency.Field test results shows: to tomato and Botrytis cinerea, and capsicum damping off, cucumber, wheat powdery mildew etc., control efficiency is remarkable, reaches more than 85%, is a kind of novel biological bactericide that has research and development value and application prospect, has good market prospects.
Own azoles alcohol (hexaconazole) belongs to triazole bactericidal agent, sterol demethylation inhibitor, and the disease that fungi especially Basidiomycota and Ascomycota are caused has protection and the therapeutic action of broad spectrum activity.Destroy and stop the biosynthesis of the cell membrane important composition composition ergosterol of germ, cause cell membrane not form, make germ dead.There is interior suction, protection and therapeutic activity.This composition can be prevented and treated disease due to sac fungi, basidiomycetes and imperfect fungus effectively, and the disease especially Basidiomycetes and Ascomycetes being caused has excellent protection and eradicant action as powdery mildew, rust, scab, brown spot, anthracnose etc.Rice sheath blight disease is had to good preventive effect.
Jiean-peptide and two kinds of active ingredients of own azoles alcohol have extraordinary control efficiency to wheat sharp eyespot, and wheat sharp eyespot has become China wheat district regular incidence evil in recent years.After wheat is infected by the withered bacterium of line, at each growing stage, there is the symptoms such as rotten bud, the rotten stem of withered, the colored stalk of sick seedling, withered strain dead ears, and for this disease, also there is no medicament especially efficiently at present.
Summary of the invention
The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to Jiean-peptide and the composite bactericidal composition of own azoles alcohol.
Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make formulation of the present invention, comprise suspending agent and water dispersible granules.
In above-mentioned bactericidal composition, the weight ratio of Jiean-peptide and own azoles alcohol is 1:60-60:1.
In above-mentioned bactericidal composition, there is following characteristics: 1. sterilizing ability is strong, two kinds of composite synergistic effects in addition of composition; 2. low toxic and environment-friendly, safe and efficient; 3. formulation is advanced, not with an organic solvent.
Embodiment
For determining Jiean-peptide and two kinds of composite synergistic effects of active ingredient of own azoles alcohol, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:
Beak angle load bacterium (Ceratobasidium cornigerum Rogers) belongs to Basidiomycotina fungi, is the main pathogenic fungi that causes wheat sharp eyespot, and according to the effect feature of two kinds of active ingredients, we survey object by life and are decided to be this bacterium.
The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the mycelial growth of Etiology of Sharp Eyespot of Wheat bacterium.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the former medicine of own azoles alcohol of the former medicine of 70% Jiean-peptide and 95% be take acetone as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Bacterium cake with card punch (Φ 5mm) the picking Etiology of Sharp Eyespot of Wheat bacterium of sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen
50, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.
Table 1 Jiean-peptide, own azoles alcohol and the measurement result of different proportioning to wheat sharp eyespot co-toxicity coefficient thereof
Analyze above-mentioned data, can obviously find out when Jiean-peptide and own azoles alcohol are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of Etiology of Sharp Eyespot of Wheat bacterium is had to the synergistic effect of different proportion, wherein Jiean-peptide and own azoles alcohol are being that proportioning four is the most remarkable to the synergy between proportioning six between 1:10-1:1, clearly, the composite desirable medicament that becomes control wheat sharp eyespot of Jiean-peptide and own azoles alcohol.
By indoor biometrics, test and determine Jiean-peptide and two kinds of active ingredients of own azoles alcohol are composite can good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 30.5% Jiean-peptide diniconazole water dispersible granule
Example two, 20.5% Jiean-peptide hexaconazole suspending agent
Example three, 21% Jiean-peptide hexaconazole suspending agent
Example four, 44% Jiean-peptide diniconazole water dispersible granule
Example five, 18% Jiean-peptide hexaconazole suspending agent
Example six, 6% Jiean-peptide hexaconazole suspending agent
Example seven, 12% Jiean-peptide hexaconazole suspending agent
Example eight, 22% Jiean-peptide hexaconazole suspending agent
Example nine, 21% Jiean-peptide hexaconazole suspending agent
Example ten, 30.5% Jiean-peptide hexaconazole suspending agent
Example 11,15% Jiean-peptide hexaconazole suspending agent
Select above example to prevent and treat the field trial of wheat sharp eyespot, determine the effect of formula control wheat sharp eyespot; Simultaneously by the Contrast on effect with Jiean-peptide and own azoles alcohol single dose, verify the synergistic effect after composite.Concrete test method data are as follows:
6% Jiean-peptide hexaconazole suspending agent and other preparation are done the test of pesticide effectiveness of field control wheat sharp eyespot.Test site is Luoyang City, and the time is on May 12nd, 2011, test each and process three repetitions, and community randomized arrangement, each community area is 100m
2, adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each community diagonal, and every some straight ahead is determined 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
0 grade: healthy tree, blade is without symptom;
1 grade: it is vaned below 5% that symptom blade accounts for institute;
3 grades: symptom blade accounts for vaned 6%-15%;
5 grades: symptom blade accounts for vaned 16%-25%;
7 grades: symptom blade accounts for vaned 26%-50%;
9 grades: it is vaned more than 50% that symptom blade accounts for institute.
Table 36% Jiean-peptide hexaconazole suspending agent and the preventive effect of different medicament to wheat sharp eyespot
From above-mentioned data, can find out, Jiean-peptide and own azoles alcohol complex preparation after medicine 7 days preventive effects all over 74%, and Jiean-peptide single dose 7 days preventive effects after medicine are 51.21%, the preventive effect of own azoles alcohol single dose is 52.31%, compare Jiean-peptide and own azoles alcohol single dose, complex preparation preventive effect is extremely remarkable, and visible two kinds of active ingredients are composite good synergistic effect.And after medicine 21 days, all complex preparations have all surpassed 82% to the preventive effect of wheat sharp eyespot, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of wheat sharp eyespot.
Claims (3)
1. a bactericidal composition, is characterized in that: active component is comprised of Jiean-peptide and own azoles alcohol, and the weight ratio of described Jiean-peptide and own azoles alcohol is 1:60～60:1.
2. bactericidal composition according to claim 1, is characterized in that: by active ingredient and auxiliary material, make suspending agent, water dispersible granules.
3. according to claim 1 or bactericidal composition claimed in claim 2, it is characterized in that: for preventing and treating wheat sharp eyespot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701369.0A CN103651396B (en) | 2013-12-18 | 2013-12-18 | Sterilization composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701369.0A CN103651396B (en) | 2013-12-18 | 2013-12-18 | Sterilization composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103651396A true CN103651396A (en) | 2014-03-26 |
| CN103651396B CN103651396B (en) | 2015-01-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310701369.0A Active CN103651396B (en) | 2013-12-18 | 2013-12-18 | Sterilization composition |
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| Country | Link |
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| CN (1) | CN103651396B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794846A (en) * | 2014-12-31 | 2016-07-27 | 苏虎成 | Compound emulsifiable concentrate for prevention and treatment of wheat stripe rust |
| CN105794556A (en) * | 2014-12-31 | 2016-07-27 | 苏虎成 | Pesticide application method for prevention and treatment of wheat sharp eyespot |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59212416A (en) * | 1983-05-19 | 1984-12-01 | Ajinomoto Co Inc | Antifungal agent for agricultural purpose |
| CN1699585A (en) * | 2004-05-20 | 2005-11-23 | 中国科学院成都生物研究所 | Process for preparing biological antibacterial agent |
| CN102286080A (en) * | 2010-06-18 | 2011-12-21 | 中国科学院成都生物研究所 | Preparation method of iturin A |
-
2013
- 2013-12-18 CN CN201310701369.0A patent/CN103651396B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59212416A (en) * | 1983-05-19 | 1984-12-01 | Ajinomoto Co Inc | Antifungal agent for agricultural purpose |
| CN1699585A (en) * | 2004-05-20 | 2005-11-23 | 中国科学院成都生物研究所 | Process for preparing biological antibacterial agent |
| CN102286080A (en) * | 2010-06-18 | 2011-12-21 | 中国科学院成都生物研究所 | Preparation method of iturin A |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105794846A (en) * | 2014-12-31 | 2016-07-27 | 苏虎成 | Compound emulsifiable concentrate for prevention and treatment of wheat stripe rust |
| CN105794556A (en) * | 2014-12-31 | 2016-07-27 | 苏虎成 | Pesticide application method for prevention and treatment of wheat sharp eyespot |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103651396B (en) | 2015-01-28 |
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Inventor after: Liu Xiaojiao Inventor after: Yang Huiying Inventor after: Wu Yong Inventor after: Shen Dongxia Inventor after: Luo Xueting Inventor after: Li Qiumei Inventor after: Xu Bo Inventor before: Liu Xiaojiao Inventor before: Yang Huiying Inventor before: Wu Yong |
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Effective date of registration: 20171218 Address after: 063600 Leting County Economic Development Zone, Tangshan City, Hebei Patentee after: Yanhua Yongle (Leting) Biological Technology Co. Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Patentee before: Beijing Yanhua Yongle Biotechnology Co., Ltd. |