CN103638554A - Preparation method of composite material of silk fibroin and marine mussel adhesion protein - Google Patents
Preparation method of composite material of silk fibroin and marine mussel adhesion protein Download PDFInfo
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- CN103638554A CN103638554A CN201310562721.7A CN201310562721A CN103638554A CN 103638554 A CN103638554 A CN 103638554A CN 201310562721 A CN201310562721 A CN 201310562721A CN 103638554 A CN103638554 A CN 103638554A
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Abstract
The invention relates to a preparation method of a composite material of silk fibroin and marine mussel adhesion protein, and discloses a preparation method of a multifunctional material composited by silk fibroin and marine mussel adhesion protein. The preparation method comprises the following steps: dissolving regenerated silk fibroin and a certain ratio of marine mussel adhesion protein in a certain amount of organic solvents, continuously stirring for well mixing so as to prepare a mixed solution of silk fibroin and marine mussel adhesion protein with a certain concentration, and preparing membranes or stents by a certain amount of the mixed solution. The whole treatment process of the preparation method of the invention has the advantages of low energy consumption, high biosecurity, low price, simple and convenient operation, no environment pollution and the like; Composite nanofiber membranes of biological fibroin and marine mussel adhesion protein prepared by the method of the invention has strong mechanical properties.
Description
Technical field
The present invention relates to the preparation method of a kind of fibroin albumen and marine mussel attachment proteins composite, belong to the processing method of fibroin albumen.
Background technology
Silkworm albumen, tussah silk fibroin are fibrin, because of its unique mechanical property and good biocompatibility, degradability and stability, and essence is the construction features of protein, be widely used in the fields such as medicine, biomaterial, be a kind of desirable bone renovating bracket material.And good cell adhesion characteristic and high-intensity mechanical property are the essential conditions of bone renovating bracket material.
Marine mussel attachment proteins has high strength, high tenacity and water proofing property, and the extremely strong function of sticking matrix, the interchain linkage of this molecular structure special with it, DOPA (DOPA) mediation and and ground between interaction mode relevant.Mussel attachment proteins is a series of alkaline proteins, the aminoacid of hydroxyl is up to 60%-70%, wherein proline (Pro) most of after translation hydroxylating be modified to urinary hydroxyproline (Hyp), stablize between polyphenol protein and and tropocollagen molecule between interaction.Catechol group has very strong metal-chelating ability in DOPA (DOPA), can form reversible metal-organic complex with metal, and catechol can form covalent bonds with a lot of radical reactions after being oxidized to quinone.This strong covalent bond of DOPA and coordination ability are the main causes that mussel adhesion protein has superpower adhesive force.And, it also has good biocompatibility and degradability waterproof and avirulence, therefore be the biological adhesive of a class very advantageous and potentiality, the field such as, artificial skelecton bonding at soft tissue, knochenbruch joint, Oral Repair, ophthalmologic operation and shipbuilding, coating is with a wide range of applications.Biopolymers company starts directly from mussel pedal gland, to extract and be developed into the adhesive product " Cell-Tak " that tissue culture uses from the eighties in 20th century, this product is mainly mfp-1 in mussel attachment proteins, the mixture of mfp-2 and mfp-3, can be used for sticking of non-adherent cell and culture dish in cell cultivation process, as can be seen here, the impact of marine mussel attachment proteins on material cell adhesion function.
When regenerated silk and marine mussel attachment proteins mixed dissolution are in organic solvent or aqueous solution, marine mussel attachment proteins occurs to be cross-linked by its strong covalent bond and fibroin albumen, significantly improves the mechanical property of fibroin composite; And marine mussel attachment proteins can improve the biocompatibility of fibroin composite, improve the adhesive capacity of composite to cell.
Summary of the invention
The improvement that the present invention has done for the deficiencies in the prior art part just, provides a kind of technique simple, processes conveniently and can provide the preparation method of the compound multifunctional material of fibroin albumen and marine mussel attachment proteins.
To achieve these goals, concrete technical scheme of the present invention is as follows:
The invention discloses the preparation method of the compound multifunctional material of a kind of fibroin albumen and marine mussel attachment proteins, concrete preparation process is as follows:
(1), regenerated silk fibroin and a certain proportion of marine mussel attachment proteins are dissolved in a certain amount of organic solvent, constantly stir and make its mix homogeneously, be made into finite concentration, can make the mixed solution of fibroin albumen and marine mussel attachment proteins;
(2), get a certain amount of mixed solution masking or support.
As further improvement, the present invention substitutes the organic solvent in claim 1 with aqueous solution.
As further improvement, the present invention prepares alternative a certain amount of mixed solution masking or the support got of nano fibrous membrane with getting a certain amount of mixed solution by electrostatic spinning technique.
As further improvement, fibroin albumen of the present invention is regeneration silk fibroin protein or regeneration tussah silk fibroin.
As further improvement, organic solvent of the present invention is hexafluoroisopropanol or Hexafluoro acetone or formic acid or trifluoroacetic acid.
As further improvement, the thin film that the present invention is made or support have good biological activity and the compatibility, and cell adhesion is had to some superiority.
As further improvement, fibroin albumen of the present invention and marine mussel attachment proteins composite pass through the multiplephase mutual effect of covalent bond/non-covalent bond by the made nano fibrous membrane of electrostatic spinning technique and the DOPA in marine mussel attachment proteins, can effectively improve the mechanical property of composite.
Compared with prior art, the present invention has following outstanding feature:
(1) it is low that the present invention has power consumption in whole processing procedure, and biological safety is high, cheap, simple to operation, the advantages such as environmentally safe;
(2) the biological fibroin making by this invention and marine mussel attachment proteins composite membrane and timbering material have good biocompatibility.Because marine mussel attachment proteins contains a certain amount of DOPA (DOPA), and the non-covalent bonding forming between the oxidation of phenolic group in DOPA molecule and the DOPA molecule that mediated thus and the extraneous surface of solids is coupled and has strong cohesive.So the composite that contains certain proportion marine mussel attachment proteins has extraordinary cell adhesion characteristic;
(3) the biological fibroin making by this invention and marine mussel attachment proteins composite nano-fiber membrane have stronger mechanical property.Because the DOPA in marine mussel attachment proteins, by the multiplephase mutual effect of covalent bond/non-covalent bond and silk fibroin material, can improve the mechanical property of nano fibrous membrane.
The specific embodiment
The present invention mainly implements by the following two kinds of programs:
(1) regenerated silk fibroin and a certain proportion of marine mussel attachment proteins are dissolved in a certain amount of organic solvent or aqueous solution, constantly stir and make its mix homogeneously, be made into finite concentration, can make the mixed solution of fibroin albumen and marine mussel attachment proteins.Get a certain amount of mixed solution masking, support or prepare nano fibrous membrane by electrostatic spinning technique.
(2) regenerated silk is dissolved in a certain amount of organic solvent or aqueous solution, is made into finite concentration.Get a certain amount of mixed solution masking, support or prepare nano fibrous membrane by electrostatic spinning technique.Then the film of making, support and nano fibrous membrane are soaked in certain density marine mussel attachment proteins solution, after certain hour, take out and dry.
Below by specific embodiment, technical scheme of the present invention is described in further detail, following examples are explanation of the invention and the present invention is not limited to following examples.
Embodiment 1
Take the marine mussel attachment proteins powder of regeneration silk fibroin protein 1g and 10mg, be placed in flat vial, add a certain amount of hexafluoroisopropanol solution, being made into concentration is 7.5% solution, constantly stir after its mix homogeneously, taking-up 7ml is applied on diameter 10cm plastic plate and naturally dries.By cell experiment, test, regenerated silk is stronger than single regenerated silk material cell adhesion performance with composite membrane, the timbering material of marine mussel attachment proteins.
Embodiment 2
Take the marine mussel attachment proteins powder of regeneration silk fibroin protein 1g and 10mg, be placed in flat vial, add a certain amount of hexafluoroisopropanol solution, being made into concentration is 7.5% solution, constantly stir after its mix homogeneously, mixed solution, as for carrying out Electrospun in electrostatic spinning device, is obtained to nano fibrous membrane and carries out Mechanics Performance Testing, and this composite electrostatic spinning film that contains marine mussel attachment proteins is more eager to excel in whatever one does than single fibroin electrostatic spinning film dynamic performance.
Embodiment 3
Take regeneration silk fibroin protein 1g, be placed in flat vial, add a certain amount of formic acid solution, being made into concentration is 10% solution, and after its mix homogeneously, taking-up 7ml is applied on diameter 10cm plastic plate and naturally dries; Or make the timbering material of certain pattern; Or by mixed solution as for carrying out Electrospun in electrostatic spinning device, obtain nano fibrous membrane; Again this composite membrane, support, nano fibrous membrane are soaked in to 24h in 10% marine mussel attachment proteins solution, take out afterwards nature and dry, respectively test cell experiment and mechanical property.
Finally, it is also to be noted that, what more than enumerate is only several specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (7)
1. a preparation method for the compound multifunctional material of fibroin albumen and marine mussel attachment proteins, is characterized in that, concrete preparation process is as follows:
(1), regenerated silk fibroin and a certain proportion of marine mussel attachment proteins are dissolved in a certain amount of organic solvent, constantly stir and make its mix homogeneously, be made into finite concentration, can make the mixed solution of fibroin albumen and marine mussel attachment proteins;
(2), get a certain amount of mixed solution masking or support.
2. the preparation method of the compound multifunctional material of fibroin albumen according to claim 1 and marine mussel attachment proteins, is characterized in that, with aqueous solution, substitutes the organic solvent in claim 1.
3. the preparation method of the compound multifunctional material of fibroin albumen according to claim 1 and marine mussel attachment proteins, it is characterized in that, with getting a certain amount of mixed solution, by electrostatic spinning technique, prepare alternative a certain amount of mixed solution masking or the support got of nano fibrous membrane.
4. according to the preparation method of the fibroin albumen described in claim 1 or 2 or 3 and the compound multifunctional material of marine mussel attachment proteins, it is characterized in that, described fibroin albumen is regeneration silk fibroin protein or regeneration tussah silk fibroin.
5. the preparation method of the compound multifunctional material of fibroin albumen according to claim 2 and marine mussel attachment proteins, is characterized in that, described organic solvent is hexafluoroisopropanol or Hexafluoro acetone or formic acid or trifluoroacetic acid.
6. the preparation method of the compound multifunctional material of fibroin albumen according to claim 1 and 2 and marine mussel attachment proteins, it is characterized in that, made thin film or support have good biological activity and the compatibility, and cell adhesion is had to some superiority.
7. the preparation method of the compound multifunctional material of fibroin albumen according to claim 3 and marine mussel attachment proteins, is characterized in that, made nano fibrous membrane has stronger mechanical property.
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| CN105327398A (en) * | 2014-08-07 | 2016-02-17 | 北京纳通科技集团有限公司 | Antimicrobial coating composition, medical implant material containing antimicrobial coating and preparation method of medical implant material |
| CN105457085A (en) * | 2016-01-25 | 2016-04-06 | 华中农业大学 | Collagen-based mussel bionic adhesive hydrogel and preparation method thereof |
| CN105617446A (en) * | 2014-10-30 | 2016-06-01 | 北京纳米能源与***研究所 | Hemostatic composite material and preparation method therefor |
| WO2017101027A1 (en) * | 2015-12-15 | 2017-06-22 | 江阴市本特塞缪森生命科学研究院有限公司 | Modified biomedical material product |
| WO2017101022A1 (en) * | 2015-12-15 | 2017-06-22 | 江阴市本特塞缪森生命科学研究院有限公司 | Modified medical scaffold material |
| CN108744023A (en) * | 2018-06-15 | 2018-11-06 | 福州大学 | A kind of fibroin albumen medical bio adhesive and preparation method thereof |
| CN108744055A (en) * | 2018-06-15 | 2018-11-06 | 福州大学 | A kind of fibroin albumen bone-cement biological adhesive and preparation method thereof |
| CN110711264A (en) * | 2018-06-26 | 2020-01-21 | 杨佼佼 | Composite material, medical adhesive, preparation method and application thereof |
| CN111991608A (en) * | 2020-08-27 | 2020-11-27 | 振德医疗用品股份有限公司 | Wound surface covering and preparation method thereof |
| CN111995777A (en) * | 2019-05-31 | 2020-11-27 | 中国科学院青岛生物能源与过程研究所 | Preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength |
| CN114748671A (en) * | 2022-03-22 | 2022-07-15 | 德晟康(苏州)生物科技有限公司 | Compound protein slow-release hydrocolloid patch and preparation method thereof |
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| CN105088201B (en) * | 2014-05-14 | 2017-11-21 | 北京纳通科技集团有限公司 | The magnesium or surface treatment method of Mg alloy of a kind of controllable degradation speed |
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| WO2017101027A1 (en) * | 2015-12-15 | 2017-06-22 | 江阴市本特塞缪森生命科学研究院有限公司 | Modified biomedical material product |
| CN105457085B (en) * | 2016-01-25 | 2018-03-02 | 华中农业大学 | A kind of bionical adhesion hydrogel of collagen-based mussel and preparation method thereof |
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| CN108744023A (en) * | 2018-06-15 | 2018-11-06 | 福州大学 | A kind of fibroin albumen medical bio adhesive and preparation method thereof |
| CN108744055A (en) * | 2018-06-15 | 2018-11-06 | 福州大学 | A kind of fibroin albumen bone-cement biological adhesive and preparation method thereof |
| CN108744055B (en) * | 2018-06-15 | 2021-04-27 | 福州大学 | Silk fibroin bone cement biological adhesive and preparation method thereof |
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