CN103630681A - Test strip card based on quantum dot labeling - Google Patents
Test strip card based on quantum dot labeling Download PDFInfo
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- CN103630681A CN103630681A CN201310558612.8A CN201310558612A CN103630681A CN 103630681 A CN103630681 A CN 103630681A CN 201310558612 A CN201310558612 A CN 201310558612A CN 103630681 A CN103630681 A CN 103630681A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
Abstract
The invention relates to the field of in-vitro diagnosis, particularly a test strip card based on quantum dot labeling with tested object standard curve, coefficient parameters and other detection information, which can quickly quantify sample single component or multicomponent concentration in combination with an instrument with signal detection function. The test strip card for detecting samples has is simple and quick, and has the characteristics of high sensitivity, objective result, high use flexibility and the like.
Description
Technical field
The invention belongs to in-vitro diagnosis field, be specifically related to a kind of self with the information for detection such as checking matter typical curve or coefficient parameter, in conjunction with thering is the instrument energy fast quantification sample one-component of signal testing function or the quantum dot-labeled strip card of polycomponent concentration.
Background technology
Colloidal gold immunochromatographimethod technology (Gold-Immunochromatography Assay, GICA) various aspects of immune detection have now been widely used in, it take miillpore filter as carrier, utilize the capillarity of miillpore filter, make the fluid sample dripping in film bar one end slowly to the miillpore filter other end, ooze and move, if have specific antigen or antibody in liquid, they can with Immuno gold in conjunction with after the corresponding antibodies on being coated on miillpore filter or antigen are combined and are shown Immuno gold color (redness) again.This technology is simple and quick, a few minutes the result that just can detect by an unaided eye.Weak point is: (1) can only qualitative detection sample, and it is quantitative that difficulty can realize sample.(2) difficulty can accomplish that sample polycomponent detects simultaneously, and detection efficiency is low.(3) for some antigen or the extremely low sample of antibody content, collaurum very slight color is difficult to the naked eye judged result, and detection sensitivity is low.
Realize sample polycomponent Quantitative detection, be the long-term targets of pursuing of people always.The nano-quantum point of developed recently (quantum dots, QDs), has unique good optical property: fluorescence radiation efficiency is high, exciting line wide ranges, energy " elementary excitation, polynary transmitting ", spectral line of emission narrow range and symmetry, photobleaching speed is slow, fluorescence lifetime is long, and particle diameter and biomolecule are close, energy multifunction after finishing, the characteristic wavelength fluorescence spectrum that the quantum dot potpourri of different-grain diameter and kind produces is not overlapping, is very suitable for sample multicomponent analysis.Sweeny etc. by three kinds of primary antibodies respectively with quantum dot (the λ em=705nm of three kinds of different emission, 605nm and 655nm) by specific binding formation antibody-quantum dot compound of biotin-avidin, when having realized in tonsillotome 3 kinds of different antibodies by fluorescence imaging, analyze, prove that employing multicolor quantum dot can locate multiple biomarker in same sample simultaneously.Quantum dot-labeled 4 kinds of antibody of 4 kinds of different emission of the use such as Nie have been realized the Reed-Sternberg to the significant cell HRS(Hodgkin ' of pathology in Hodgkin lymphoma s and) imaging detect, improve detection sensitivity and specificity, be conducive to the clinical Hodgkin lymphoma of diagnosing rapidly and accurately.Kobayashi etc. and lymphatic vessel stream and the left and right thoracic cavity of Chan philosophy with the near-infrared quantum dots imaging analysis mouse of two kinds of different colours, proved the multiple imaging dissection of near-infrared quantum dots.Utilize the fluorescent characteristic of quantum dot can also realize the qualitative and quantitative analysis to multiple pathogens, toxin, antigen, antibody, enzyme, small-molecule substance and ion etc. except carrying out imaging analysis.Zahavy etc. prepare fluorescence probe QD585-IgG α B.anthracis and QD655-IgG α Y.pestis, and the simultaneous quantitative of having realized two kinds of pathogenic bacteria in conjunction with flow cytometer detects, and detectability can reach 10
3cfu/mL.When having realized enterotoxin B that staphylococcus produces and TNT and cholera toxin, ricin (WA), shiga toxin and staphylococcal entotoxin four kinds of toxin with color quantum point, Goldman seminar detects.In addition, in microfluid protein-chip, with quantum dot, make fluorescence labeling, utilize its polyfluoro chromatism fast detecting tumor markers carcinomebryonic antigen (CEA) and alpha-fetoprotein (AFP) simultaneously, detectability is low to moderate 250fmol/L, than detect high 4 orders of magnitude with organic fluorescent dye.Giorgio etc. form fluorescence probe with the quantum dot of different emission respectively by VEGF-A (VEGF A) and angiogenin (angiopoietin), by antigen and antibody specific association reaction, cause the gathering of quantum dot, thereby with the quantitative detected characteristics fluorescence signal of flow cytometer, obtain the concentration of corresponding antigens, detection is limited to 1pmol/L, when having realized plurality of antigens, detects.In the last few years, many researchers utilized the method for quantum-dot coding to carry out multicomponent analysis.This technology is mainly to encode by the rich of quantum dot fluorescence color and fluorescence intensity, can measure more component simultaneously, can realize the Synchronization Analysis of the magnanimity information to containing in the researchs such as protein analysis, DNA analysis and drug screening.In theory,
nplant intensity
mplanting color can produce
n m -a kind of code, utilizes decoding technique to realize right
n m the analyzing and testing of-a kind of object.According to calculating, only need combine from the quantum dot nano particle of 6 kinds of different luminous intensities by 5～6 kinds of colors, just can obtain 10000～40000 discernible nano particle coding microballs.If combined from the quantum dot nano particle of 10 kinds of different luminous intensities, can obtain 1,000,000 discernible nano particle coding microballs, these microballoons could carry out coded markings to 1,000,000 different DNA or protein in theory, thereby realize, 1,000,000 different DNA in sample or protein are detected simultaneously! Nie etc. wrap up the quantum dot of three kinds of different colours of red, green, blue into polystyrene microsphere and encode with the ratio of 1:1:1,1:2:1,2:1:1, when having realized three kinds of target dnas by the different qualities of gained fluorescence spectrum, detect.Adopt similar techniques, Gu and Sha etc. encode the quantum dot of two kinds of different emission to have realized respectively the quantitative detection of multiple not homotactic single stranded DNA and the high flux screening somatotype of multiple oligonucleotides to the hydrogel of DNA responsive type and microballoons respectively with three kinds of intensity.When having realized three kinds of haemocyanins (chicken ovalbumin OVA, bovine serum albumin(BSA) BSA, human serum albumins HAS) and four kinds of immunoglobulin Gs (human IgG, rabbit igg, mouse IgG, sheep IgG), this technology of utilizing Wilson and Cooper philosophy detects.
China's application number patent 200410010736.3, with colloidal gold labeled monoclonal antibody, be combined with object, by immune response coated quantum dot-labeled antibody on film, be combined, form and detect band, with the naked eye to detecting band, carrying out on the basis of preliminary qualitative or half-quantitative detection, the fluorescent quenching effect of recycling collaurum to fluorescent nano particles, quantitatively detects test strip by detecting the cancellation degree of fluorescence signal.This patent will be with two kinds of particle marks, and method is simple not; Adopt the cancellation degree of fluorescence signal quantitatively to detect, disturbing factor is many, and detection sensitivity is low.
China's application number patent 200610024086.7 has disclosed a kind of detection method of quantum dot mark fast immune chromatographic test paper bar.Though this patent can realize polycomponent and detect simultaneously, what ask for protection is a kind of method, and the protection of not filing a request is based on quantum dot mark fast immune chromatographic test paper bar product.And, the method Shortcomings: (1) realizes the detection of sample polycomponent need to arrange many corresponding detection lines on analyzing film simultaneously, and every detection line is only for detection of a kind of index in sample, and detection efficiency is low.If tester wants synchronously to detect in sample a large amount of components, flux component, magnanimity component even, a slight strip can not be realized detection target by so numerous corresponding detection lines are set.(2) thus adopt ultra violet lamp ELISA test strip band and quality control band with observe its photoluminescence line and have or not or power judgement sample in whether contain object, truly say, this is still a kind of qualitative detection, or can only reach at the most the detectable range of certain checking matter is measured, can not fundamentally realize the accurate concentration detection of component arbitrarily in sample, sensitivity is still low.
Adopting the quantitative checking matter concentration of checking matter standard items typical curve, is the main method of the quantitative sample concentration of detection field.Message area is the storage medium of development recently, as RFID label (claiming again RFID tag), IC chip, magnetic code, bar code etc., for Information Access identification, the fields such as computing machine, communication, electronics, business, communications and transportation control and management have now been widely used in, wherein, RFID label especially, volume is little, storage information capacity is large, identification need not manual intervention, the desirable means of Information Access identification especially, but there be limited evidence currently of has people to use it for biomedical detection.
The present invention is directed to shortcomings and deficiencies of the prior art, a kind of quanta dot mark test bar card that storage medium 12 is installed on it is provided, and this storage medium 12 stores the detection information such as the typical curve used with the quantitative checking matter concentration of batch quanta dot mark test bar 2 or coefficient parameter.Sample completes after strip reaction on strip 2, gathers the interior strips 2 of strip card cartridge 3 detect with 6 and the characteristic signal of quality control band 7 read the checking matter typical curve that comes or coefficient parameter and calculate and obtain sample single component or polycomponent concentration from storage medium 12 in conjunction with this instrument simultaneously by the instrument with signal testing function.The present invention for quantitative sample concentration particularly sample polycomponent concentration have easy quick, highly sensitive, result is objective, use the features such as flexible.
Summary of the invention
Technical solution of the present invention is as follows:
Quantum dot-labeled strip card of the present invention, comprises the quanta dot mark test bar 2 of cartridge 3 and Qi Nei.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on sample pad 4, label pad 5, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.The upper box face of cartridge 3 is having well 10 corresponding to strip sample pad 4 positions, and the upper box face of cartridge 3 is having detection window 11 corresponding to strip analyzing film 8 positions.One storage medium 12 is installed in described cartridge 3.
The label pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single special molecular, or: each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant special molecular.Label pad 5 coated described single quantum dots comprise ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one, also can be many.Detection is coated with a certain target checking matter with 6 and detects another relevant special molecular, or is coated with the potpourri that each target checking matter detects another relevant special molecular.
The quality control band 7 of described quanta dot mark test bar 2 is coated with Quality Control thing.Described Quality Control thing comprises that two is anti-.
Described storage medium 12 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.Storage medium 12 stores the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, strip quality control band optical density reference monitor value, strip lot number, strip is imitated phase, storage medium password, clinical indices reference value, strip production firm information etc., and can be read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 12 stores has various ways available, and it includes but not limited to following curve: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve.Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration.
After the adsorptive pads 9 of quanta dot mark test bar 2 of the present invention, can also be overlapped with a strip reaction end indicating label 13.Correspondingly, these strip reaction end indicating label 13 corresponding positions of its cartridge 3 have view window 14.Described strip reaction end indicating label 13 comprises that color change interval is the pH test paper of 5-9.
The label pad 5 of quanta dot mark test bar 2 of the present invention is glass fibre membrane; The analyzing film 8 of described quanta dot mark test bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
During sample detection, the strip that is applied with sample has been stuck in after the reaction of strip chromatography, by the instrument with signal testing function, gather its strip 2 and detect with 6 and the characteristic signal of quality control band 7, and read the corresponding checking matter typical curve that comes or coefficient parameter simultaneously and calculate and obtain sample single component or polycomponent concentration from storage medium 12 in conjunction with this instrument.
The sample to be checked of quanta dot mark test bar card of the present invention can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
Compared with prior art, the present invention has following beneficial effect:
(1) described strip card can be realized sample polycomponent in conjunction with the instrument (as reading bar instrument, fluorescence detector etc.) with signal testing function and quantitatively detect simultaneously and rapidly (comprising qualitative detection).The quantum dot of different-grain diameter, kind and structure can produce different characteristic wavelength and continue fluorescence, the characteristic wavelength fluorescence peak spectrum that quantum dot potpourri produces is not overlapping, the present invention adopts different quantum dots mark checking matter respective reaction molecule respectively, its potpourri is coated on the strip 2 of described strip card and reacts with testing sample, by measuring strip 2 characteristic fluorescence signals, thereby can fast quantification sample polycomponent concentration.
(2) detect the sample time short, a few minutes just can obtain result.The quantitative sample concentration of the present invention does not need operator to make checking matter typical curve again, and the typical curve of checking matter or coefficient parameter etc. detect with on the standby storage storage medium 12 of information.During sample detection, operator only need insert the instrument with signal testing function by the strip card that is applied with sample after it completes strip reaction, by this instrument, gathering its strip 2 detects with 6 and the characteristic fluorescence signal of quality control band 7, in conjunction with this instrument, from storage medium 12, read corresponding checking matter typical curve or the coefficient parameter come, a few minutes just can quantitatively obtain sample detection result simultaneously.
(3) can carry out dynamic monitoring to strip chromatography course of reaction.The present invention detects the Reinhoit Zahl (OD of band and quality control band according to strip
detect band/ OD
quality control bandor OD
detect band/ (OD
detect band+ OD
quality control band)) or coefficient parameter and in conjunction with the checking matter typical curve of storage medium 12 storage by instrument (as reading bar instrument, fluorescence detector etc.) the Analysis deterrmination checking matter concentration with signal testing function.The quality control band optical density reference monitor value (OD of checking matter standard items while also storing standard curve making on storage medium 12
quality control band ').If the failure of strip detection reaction, the quality control band optical density value (OD that testing process obtains
quality control band) can with the quality control band optical density reference monitor value (OD of storage medium 12 storage
quality control band ') there is very big statistical errors, prompting strip reacts unsuccessfully.Typical curve on storage medium 12 and OD
quality control band 'match with strip 2 in strip card, the checking matter concentration checkout discrepancy that may cause because of batch mass discrepancy to overcome the built-in strip 2 of strip card.The tail end of strip 2 of the present invention also can be provided with strip reaction end indicating label 13, this label be used to refer to used instrument its reactant on 2 before strip 2 detects whether fully ooze move to strip detect be with 6 and quality control band 7 to be applicable to use instrument can to strip detection be with 6 and quality control band 7 carry out autoscan, thereby reach the success or failure details of dynamic monitoring strip chromatography course of reaction.
(4) detect quality and adopt multiple indexes synchro control, make sample quantitatively more accurate: 1. on strip 2, quality control band 7 is set, its optical density intensity works the contrast effect that detects.2. storage medium 12 is stored corresponding checking matter typical curve or coefficient parameters, for quantitative corresponding checking matter concentration.3. storage medium 12 is stored strip quality control band optical density reference monitor value (OD
quality control band '), for monitoring the reaction of sample detection strip, whether set up, point out testing result whether effective.4. strip reaction end indicating label is set on strip 2, and the siphon of prompting strip reactant on strip oozes whether move chromatography abundant, and whether reaction reaches home.
(5) amount of samples is few, and a few microlitre to tens microlitres just can meet detection needs.
(6) in strip card, reagent and material do not relate to active inactivation, can be in room temperature long preservation.
Accompanying drawing explanation
Figure l: the plan structure figure of quanta dot mark test bar card preferred version one of the present invention (showing that in it, strip adopts a detection line)
Fig. 2: the side-looking structural drawing of strip in the cartridge of quanta dot mark test bar card preferred version one of the present invention (showing that strip adopts a detection line)
Fig. 3: the plan structure figure of quanta dot mark test bar card preferred version one of the present invention (showing that in it, strip adopts many detection lines)
Fig. 4: the side-looking structural drawing of strip in the cartridge of quanta dot mark test bar card preferred version one of the present invention (showing that strip adopts many detection lines)
Fig. 5: the plan structure figure of another preferred version of quanta dot mark test bar card of the present invention (showing that in it, strip adopts a detection line)
Fig. 6: the side-looking structural drawing of strip in the cartridge of another preferred version of quanta dot mark test bar card of the present invention (showing that strip adopts a detection line)
Fig. 7: the plan structure figure of another preferred version of quanta dot mark test bar card of the present invention (showing that in it, strip adopts many detection lines)
Fig. 8: the side-looking structural drawing of strip in the cartridge of another preferred version of quanta dot mark test bar card of the present invention (showing that strip adopts many detection lines)
Sequence number is expressed as follows:
1, end liner, 2, strip, 3, cartridge, 4, sample pad, 5, label pad, 6, detect band, 7, quality control band, 8, analyzing film, 9, adsorptive pads, 10, well, 11, detection window, 12, storage medium, 13, strip reaction end indicating label, 14, view window.
Embodiment
Embodiment and accompanying drawing thereof are only that those skilled in the art should not limit the scope of the invention with this in order to further illustrate the present invention below.
embodiment mono-
Fig. 1-4 explanation embodiment mono-.Embodiment mono-is one of quanta dot mark test bar card preferred version of the present invention.
In Fig. 1-4, the described quanta dot mark test bar card of described preferred version comprises cartridge 3 and quanta dot mark test bar 2.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on sample pad 4, label pad 5, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.The upper box face of cartridge 3 is having well 10 corresponding to strip sample pad 4 positions, and the upper box face of cartridge 3 is having detection window 11 corresponding to strip analyzing film 8 positions.One storage medium 12 is installed in cartridge 3.
The label pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single special molecular; Or: each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant special molecular.The following particle of the optional use of described single quantum dot of strip 2 label pad 5, comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one (shown in Fig. 1 and Fig. 2), can be also many (shown in Fig. 3 and Fig. 4).Detection is coated with a certain target checking matter with 6 and detects another relevant special molecular; Or: be coated with the potpourri that each target checking matter detects another relevant special molecular.
The quality control band 7 of described quanta dot mark test bar 2 is coated with Quality Control thing.Quality Control thing comprises that two is anti-.
Described storage medium 12 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.It stores the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, strip quality control band optical density reference monitor value, strip lot number, strip is imitated phase, storage medium password, clinical indices reference value, strip production firm information etc., and can be read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 12 stores has various ways available, and it includes but not limited to following curve: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve.Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration.
The label pad 5 of described quanta dot mark test bar 2 is glass fibre membrane; The analyzing film 8 of described quanta dot mark test bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
During sample detection, described quanta dot mark test bar card gathers its strip 2 by the instrument with signal testing function and detects with 6 and the characteristic signal of quality control band 7, and reads the checking matter typical curve that comes or coefficient parameter simultaneously and calculate and obtain sample single component or polycomponent concentration from storage medium 12 in conjunction with this instrument.
The sample to be checked of described quanta dot mark test bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
embodiment bis-
Fig. 5-8 explanation embodiment bis-.Embodiment bis-is another preferred version of quanta dot mark test bar card of the present invention.
In Fig. 5-8, the described quanta dot mark test bar card of described preferred version comprises cartridge 3 and quanta dot mark test bar 2.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on sample pad 4, label pad 5, analyzing film 8, adsorptive pads 9, the strip reaction end indicating label 13 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.The upper box face of cartridge 3 is having well 10 corresponding to strip sample pad 4 positions, the upper box face face of cartridge 3 is having detection window 11 corresponding to strip analyzing film 8 positions, the upper box face of cartridge 3, having view window 14 corresponding to strip reaction end indicating label 13 positions, is provided with a storage medium 12 in cartridge 3.
The label pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single special molecular; Or: each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant special molecular.The following particle of the optional use of described single quantum dot of strip label pad 5, comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one (shown in Fig. 5 and Fig. 6), can be also many (shown in Fig. 7 and Fig. 8).Detection is coated with a certain target checking matter with 6 and detects another relevant special molecular; Or: be coated with the potpourri that each target checking matter detects another relevant special molecular.
The quality control band 7 of described quanta dot mark test bar 2 is coated with Quality Control thing.Quality Control thing comprises that two is anti-.
Described strip reaction end indicating label 13 includes but not limited to that color change interval is the pH test paper of 5-9.During sample detection, strip reaction end indicating label 13 on strip can develop the color according to the pH condition of strip reaction, whether with prompting strip reactant, fully ooze to move past to detect and be with 6 and quality control band 7, whether the reaction of prompting strip is abundant, whether its testing result is effective.
Described storage medium 12 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.It stores the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, strip quality control band optical density reference monitor value, strip lot number, strip is imitated phase, storage medium password, clinical indices reference value, strip production firm information etc., and can be read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 12 stores has various ways available, and it includes but not limited to following curve: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve.Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration.
The label pad 5 of described quanta dot mark test bar 2 is glass fibre membrane; The analyzing film 8 of described quanta dot mark test bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
When described quanta dot mark test bar card is used for sample detection, fluid sample is applied in the well 10 of strip card, strip reaction end indicating label 13 colour developing indication strips 2 until strip in it 2 complete after reaction, by the instrument with signal testing function, gather strip 2 and detect with 6 and the characteristic signal of quality control band 7, and read the checking matter typical curve that comes or coefficient parameter simultaneously and calculate and obtain sample single component or polycomponent concentration from storage medium 12 in conjunction with this instrument.
The sample to be checked of described quanta dot mark test bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
It is to be noted, quantum dot-labeled strip card of the present invention can also have other improvement, as it is upper outside except being arranged on the cartridge 3 of strip card with the storage medium 12 of information to store the detections such as checking matter typical curve, also can be directly installed on quanta dot mark test bar 2; Based on same principle of the present invention, the quanta dot mark test bar in cartridge 3 also can be expanded and replace with quantum dot-labeled biochip (comprising antigen-antibody chip, protein chip, nucleic acid chip, micro-fluidic chip) etc.Therefore, every to any other technical scheme that is equal to replacement or equivalent transformation formation of quanta dot mark test bar card employing of the present invention, all drop in the protection domain of the claims in the present invention.
Claims (3)
1. a quantum dot-labeled strip card, comprise cartridge (3) and the quanta dot mark test bar (2) in it, quanta dot mark test bar (2) comprises that overlap joint is fixed on the sample pad (4) on end liner (1) in turn, label pad (5), analyzing film (8), adsorptive pads (9), analyzing film (8) has the band of detection (6) and quality control band (7), detecting band (6) is one or more, the upper box face of cartridge (3) is having well (10) corresponding to strip sample pad (4) position, the upper box face of cartridge (3) is having detection window (11) corresponding to strip analyzing film (8) position, it is characterized in that:
One storage medium (12) is installed in described cartridge (3);
The label pad (5) of described quanta dot mark test bar (2) is coated with single quantum dot-labeled a certain target checking matter and detects relevant single special molecular, or: each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant special molecular;
The detection band (6) of described quanta dot mark test bar (2) is coated with a certain target checking matter and detects another relevant special molecular, or: be coated with the potpourri that each target checking matter detects another relevant special molecular;
The quality control band (7) of described quanta dot mark test bar (2) is coated with and comprises two anti-Quality Control things;
The described single quantum dot of the label pad (5) of described quanta dot mark test bar (2) comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell,
Described storage medium (12) comprises RFID label, IC chip, magnetic code or bar code;
Described storage medium (12) stores with batch quanta dot mark test bar (2) the checking matter typical curve that quantitatively sample concentration is used or coefficient parameter, strip quality control band optical density reference monitor value, strip lot number, strip effect phase, storage medium password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result information;
The checking matter typical curve that described storage medium (12) stores has various ways available, and it comprises: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve; Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration;
The label pad (5) of described quanta dot mark test bar (2) is glass fibre membrane, and the analyzing film (8) of described quanta dot mark test bar (2) is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner (1) of described quanta dot mark test bar (2) is polyester or plastic plate; Described cartridge (3) is that polyester, plastics or rigid paper material are made;
The instrument with signal testing function gathers its strip (2) and detects band (6) and the characteristic signal of quality control band (7) the checking matter typical curve simultaneously reading from storage medium (12) in conjunction with this instrument or coefficient parameter and calculate acquisition sample single component or polycomponent concentration.
2. a quantum dot-labeled strip card as claimed in claim 1, is characterized in that: the adsorptive pads (9) of wherein said quanta dot mark test bar (2) is also overlapped with a strip reaction end indicating label (13) afterwards; This strip reaction end indicating label (13) corresponding position of its cartridge (3) has view window (14);
Described strip reaction end indicating label (13) comprises that color change interval is the pH test paper of 5-9;
The described single quantum dot of the label pad (5) of described quanta dot mark test bar (2) comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell,
Described storage medium (12) comprises RFID label, IC chip, magnetic code or bar code;
Described storage medium (12) stores with batch quanta dot mark test bar (2) the checking matter typical curve that quantitatively sample concentration is used or coefficient parameter, strip quality control band optical density reference monitor value, strip lot number, strip effect phase, storage medium password, clinical indices reference value, strip production firm information, and can read in measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result information;
The checking matter typical curve that described storage medium (12) stores has various ways available, and it comprises: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve; Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration;
The label pad (5) of described quanta dot mark test bar (2) is glass fibre membrane; The analyzing film (8) of described quanta dot mark test bar (2) is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner (1) of described quanta dot mark test bar (2) is polyester or plastic plate; Described cartridge (3) is that polyester, plastics or rigid paper material are made;
The instrument with signal testing function gathers its strip (2) and detects band (6) and the characteristic signal of quality control band (7) the checking matter typical curve simultaneously reading from storage medium (12) in conjunction with this instrument or coefficient parameter and calculate acquisition sample single component or polycomponent concentration.
3. a quantum dot-labeled strip card as claimed in claim 1 or 2, it is characterized in that: sample to be checked is from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease, and non-clinical sample comprises that food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detect, the sample of illicit drugs inspection.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310558612.8A CN103630681A (en) | 2013-11-12 | 2013-11-12 | Test strip card based on quantum dot labeling |
| PCT/CN2014/089564 WO2015070699A1 (en) | 2013-11-12 | 2014-10-27 | Quantum dot-labeled test strip card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310558612.8A CN103630681A (en) | 2013-11-12 | 2013-11-12 | Test strip card based on quantum dot labeling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103630681A true CN103630681A (en) | 2014-03-12 |
Family
ID=50211946
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310558612.8A Pending CN103630681A (en) | 2013-11-12 | 2013-11-12 | Test strip card based on quantum dot labeling |
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| Country | Link |
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| WO (1) | WO2015070699A1 (en) |
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