CN103627649A - Bacillus subtilis fermentation medium - Google Patents
Bacillus subtilis fermentation medium Download PDFInfo
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- CN103627649A CN103627649A CN201310189938.8A CN201310189938A CN103627649A CN 103627649 A CN103627649 A CN 103627649A CN 201310189938 A CN201310189938 A CN 201310189938A CN 103627649 A CN103627649 A CN 103627649A
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Abstract
A disclosed bacillus subtilis fermentation medium is composed of the following components in parts by weight: 2-10 parts of wheat bran, 1-8 parts of corn flour, 1-6 parts of lactose, 0.01-0.5 part of beef extract, 0.01-0.4 part of ammonium sulfate, 0.01-0.5 part of sodium hydroxide, 20-40 parts of radix astragali aqueous solution, 1-10 parts of haw aqueous solution and 50-74 parts of double-distilled water. According to the bacillus subtilis fermentation medium provided by the inveiton, wheat bran, corn flour, lactose, beef extract, ammonium sulfate, sodium hydroxide, radix astragali aqueous solution and haw aqueous solution are compounded for preparing the fermentation medium, the good growth of bacillus subtilis is improved and the spore generating rate of bacillus subtilis is improved; and also a bacillus subtilis liquid fermented by the medium has the effect of improving immunity and promoting feed intake.
Description
Technical field
The invention belongs to microbial fermentation technology field, relate to a kind of fermentation of bacillus subtilis substratum.
Background technology
Subtilis is a kind of of bacillus, belongs to gram-positive microorganism, and without pod membrane, peritrichous, can move.Subtilis is as probiotic bacterium, have advantages of as follows: in thalli growth process, produce subtilyne, polymyxin, nystatin, linear gramicidins isoreactivity material, these active substances have obvious restraining effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; Can consume rapidly the free oxygen in environment, cause enteron aisle hypoxemia, promote useful anerobe growth, and produce organic acid, reduce enteron aisle pH value, indirectly suppress other pathogenic bacterium growth; Growing of stimulating animal immune organ, activates T, bone-marrow-derived lymphocyte, improves immunoglobulin (Ig) and antibody horizontal, strengthens cellular immunization and humoral immune function, improves herd immunity; Thalline self can synthesize the enzyme such as α-amylase, proteolytic enzyme, lipase, cellulase element, in digestive tube with animal body in digestive ferment together play a role; The multiple vitamin B group such as energy synthesise vitamins B1, B2, B6, nicotinic acid, the activity of raising animal body internal interference element and scavenger cell.Subtilis, as useful microorganism, has been obtained good effect at aspects such as purifying aquatic product cultivating environment, peaceful control of crop disease, additive for farm animal feed at present, more and more gets more and more people's extensive concerning in recent years.
But in current Industrial processes, there is the problem that spore forming rate is not high, production cost is too high in bacillus subtilis microbial agent; In microbial inoculum, gemma rate is low, there is no the thalline death at short notice of gemma, greatly reduces activity and the quality stability of microbial inoculum.
Summary of the invention
The object of this invention is to provide a kind of fermentation of bacillus subtilis substratum, can promote the good growth of subtilis, improve the product gemma rate of subtilis.
In order to realize above object, the technical solution adopted in the present invention is: a kind of fermentation of bacillus subtilis substratum, is comprised of the component of following parts by weight: 2～10 parts, wheat bran, 1～8 part of Semen Maydis powder, 1～6 part of lactose, 0.01～0.5 part of extractum carnis, 0.01～0.4 part, ammonium sulfate, 0.01～0.5 part, sodium hydroxide, 20～40 parts, Radix Astragali water, 1～10 part, hawthorn water, 50～74 parts of distilled waters.
Above-mentioned fermentation of bacillus subtilis substratum, preferably, the component by following parts by weight forms: 6 parts, wheat bran, 2 parts of Semen Maydis powder, 2 parts of lactose, 0.08 part of extractum carnis, 0.02 part, ammonium sulfate, 0.03 part, sodium hydroxide, 40 parts, Radix Astragali water, 4 parts, hawthorn water, 64 parts of distilled waters.
Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described Radix Astragali water can also be commercial goods.
Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water can also be commercial goods.
Described distilled water can be commercial goods.
Fermentation of bacillus subtilis substratum of the present invention, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution high pressure (1.05kg/cm under 121 ℃ of conditions
2) sterilizing 15min, obtain.
Fermentation of bacillus subtilis substratum of the present invention, the property of medicine drug effect that relates to traditional Chinese medicine ingredients is as follows:
The Radix Astragali: property is sweet, warm; Return lung, the spleen channel.There is the immunity system of enhancing, delay senility.Anti-oxidant, strengthen the effects such as cardiovascular systems, antithrombotic, antiviral, anti-inflammatory and analgesia; Benefiting qi and raising yang, strengthening superficial resistance to stop perspiration, inducing diuresis to remove edema, promoting pus discharge and tissue regeneration by strengthening QI; Cure mainly the card of all Qi and blood deficiencies, as diarrhea due to hypofunction of the spleen, cough due to deficiency of the lung, prolapse of the anus, uterine prolapse, spontaneous perspiration, night sweat, oedema, blood-arthralgia, ulcer is difficult bursts or bursts for a long time and do not hold back etc.
Hawthorn: property acid, sweet, tepor; Return spleen, stomach, Liver Channel.There is digestion promoting, step-down, lipopenicillinase, the effect such as anti-oxidant; Food digesting stomach fortifying, promoting the circulation of qi scattered silt; Cure mainly eating accumulation, abdominal distention, dysentery etc.
Fermentation of bacillus subtilis substratum of the present invention, wherein, wheat bran contains sporeformer grow needed vitamin B group, amino acid, trace element; Semen Maydis powder contains the sporeformer trace elements such as needed vitamin-E, calcium, phosphorus, potassium, sodium, magnesium, iron, zinc, selenium, copper, manganese of growing; Lactose provides carbon source and the energy for bacillus subtilis bacteria growing; Extractum carnis provides nitrogenous source for bacillus subtilis bacteria growing; Ammonium sulfate provides nitrogenous source for bacillus subtilis bacteria growing; Sodium hydroxide is for regulating pH value at bacillus subtilis bacterium culture medium.
Gemma is bacteriotrophy body when must nutriment being about to exhaust, the hypopus that the water content forming in cell is extremely low, resistance is extremely strong.The gemma of subtilis is ellipse or column, be positioned at thalline central authorities or slightly off normal put.The gemma of subtilis is easily preserved compared with its nourishing body cell, and resurrection rate is high, is the desirable existence form of preparing bacillus subtilis formulation; The multiple resistance such as that the gemma of subtilis has is heat-resisting, drought-enduring, uvioresistant and organic solvent, produce in the process of machine high temperature drying at interchangeable manufacturing, can guarantee the activity of microbiobacterial agent to improve its quality product.
Fermentation of bacillus subtilis substratum of the present invention, adopt wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide and Radix Astragali water, the composite fermention medium of making of hawthorn water, can promote the good growth of subtilis, improve the product gemma rate of subtilis; Use the bacillus subtilis microbial agent of this culture medium culturing to there is higher gemma rate, guaranteed the activity of microbial inoculum, improved quality and the quality stability of product; Meanwhile, use the bacillus subtilis microbial agent of this culture medium culturing to there is the effect that improves immunizing power and promote food consumption.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 2 parts, wheat bran, 8 parts of Semen Maydis powder, 1 part of lactose, 0.5 part of extractum carnis, 0.2 part, ammonium sulfate, 0.01 part, sodium hydroxide, 40 parts, Radix Astragali water, 1 part, hawthorn water, 50 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Embodiment 2
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 10 parts, wheat bran, 5 parts of Semen Maydis powder, 4 parts of lactose, 0.01 part of extractum carnis, 0.4 part, ammonium sulfate, 0.3 part, sodium hydroxide, 30 parts, Radix Astragali water, 10 parts, hawthorn water, 62 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Embodiment 3
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 8 parts, wheat bran, 1 part of Semen Maydis powder, 6 parts of lactose, 0.2 part of extractum carnis, 0.01 part, ammonium sulfate, 0.5 part, sodium hydroxide, 20 parts, Radix Astragali water, 6 parts, hawthorn water, 74 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Embodiment 4
The fermentation of bacillus subtilis substratum of the present embodiment, is comprised of the component of following parts by weight: 6 parts, wheat bran, 2 parts of Semen Maydis powder, 2 parts of lactose, 0.08 part of extractum carnis, 0.02 part, ammonium sulfate, 0.03 part, sodium hydroxide, 40 parts, Radix Astragali water, 4 parts, hawthorn water, 65 parts of distilled waters.Described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.Described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
The fermentation of bacillus subtilis substratum of the present embodiment, is prepared by following methods: get in proportion after Radix Astragali water, hawthorn water and distilled water mixing, then add wheat bran, Semen Maydis powder, lactose, extractum carnis, ammonium sulfate, sodium hydroxide fully to mix, obtain mixed solution; By gained mixed solution autoclaving 15min under 121 ℃ of conditions, obtain.
Experimental example
This experimental example is used the fermentation of bacillus subtilis substratum of embodiment 1～4 to cultivate subtilis, and its result is as shown in table 1.
Table 1 subtilis culture experiment result
| Substratum | Incubation time/h | Produce gemma rate/% |
| Embodiment 1 | 36 | 92 |
| Embodiment 2 | 36 | 92 |
| Embodiment 3 | 36 | 93 |
| Embodiment 4 | 36 | 95 |
| Comparative example | 36 | 70 |
Wherein, comparative example Media Components is: glucose 2 weight parts, peptone 1.5 weight parts, sodium-chlor 0.5 weight part, extractum carnis 0.05 weight part, distilled water 100 weight parts.
Claims (4)
1. a fermentation of bacillus subtilis substratum, is characterized in that: the component by following parts by weight forms: 2～10 parts, wheat bran, 1～8 part of Semen Maydis powder, 1～6 part of lactose, 0.01～0.5 part of extractum carnis, 0.01～0.4 part, ammonium sulfate, 0.01～0.5 part, sodium hydroxide, 20～40 parts, Radix Astragali water, 1～10 part, hawthorn water, 50～74 parts of distilled waters.
2. fermentation of bacillus subtilis substratum according to claim 1, is characterized in that: the component by following parts by weight forms: 6 parts, wheat bran, 2 parts of Semen Maydis powder, 2 parts of lactose, 0.08 part of extractum carnis, 0.02 part, ammonium sulfate, 0.03 part, sodium hydroxide, 40 parts, Radix Astragali water, 4 parts, hawthorn water, 64 parts of distilled waters.
3. fermentation of bacillus subtilis substratum according to claim 1 and 2, is characterized in that: described Radix Astragali water is prepared by following methods: the Radix Astragali is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
4. fermentation of bacillus subtilis substratum according to claim 1 and 2, is characterized in that: described hawthorn water is prepared by following methods: hawthorn is filtered after infusion 30min in water, then repeat infusion and filter twice, merge filtrate three times, obtain.
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| CN104293690A (en) * | 2014-05-27 | 2015-01-21 | 河南牧业经济学院 | Traditional Chinese medicinal micro-ecological composition, preparation thereof, and preparation method of preparation |
| CN105174493A (en) * | 2015-10-10 | 2015-12-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof |
| CN106544304A (en) * | 2016-12-08 | 2017-03-29 | 青岛益佰农肥业有限公司 | With fixed nitrogen and the bacillus subtilis formulation for preventing and treating soil-borne disease dual-use function and preparation method thereof |
| CN108703253A (en) * | 2018-04-09 | 2018-10-26 | 华中农业大学 | A kind of feeding natural plants fermentation preparation and its preparation method and application preventing livestock and poultry |
| CN113201477A (en) * | 2021-05-10 | 2021-08-03 | 江苏绿科生物技术有限公司 | Production process of hay compound culture medium |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293690A (en) * | 2014-05-27 | 2015-01-21 | 河南牧业经济学院 | Traditional Chinese medicinal micro-ecological composition, preparation thereof, and preparation method of preparation |
| CN105174493A (en) * | 2015-10-10 | 2015-12-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof |
| CN106544304A (en) * | 2016-12-08 | 2017-03-29 | 青岛益佰农肥业有限公司 | With fixed nitrogen and the bacillus subtilis formulation for preventing and treating soil-borne disease dual-use function and preparation method thereof |
| CN108703253A (en) * | 2018-04-09 | 2018-10-26 | 华中农业大学 | A kind of feeding natural plants fermentation preparation and its preparation method and application preventing livestock and poultry |
| CN113201477A (en) * | 2021-05-10 | 2021-08-03 | 江苏绿科生物技术有限公司 | Production process of hay compound culture medium |
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