CN103609754B - Paeonia ostii seed oil with oxidative stability and preparation method thereof - Google Patents
Paeonia ostii seed oil with oxidative stability and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 241000847925 Paeonia ostii Species 0.000 title abstract description 12
- 230000001590 oxidative effect Effects 0.000 title abstract description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 284
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 claims abstract description 95
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses paeonia ostii seed oil with oxidative stability. The paeonia ostii seed oil is a mixture of a natural extract which is formed by compounding extracted fresh paeonia ostii seed oil with beta-carotene, lycopene, a rosemary extract and citric acid. The mixture comprises the following components: 0.02-0.06% of beta-carotene, 0.03-0.06% of lycopene, 0.03-0.07% of rosemary extract, 0.02-0.10% of citric acid and the balance of paeonia ostii seed oil. The invention also discloses a preparation method of the paeonia ostii seed oil. The method comprises the following steps: extracting the paeonia ostii seed oil; respectively preparing mother liquid of the citric acid, the beta-carotene, the lycopene and the rosemary extract; adding the citric acid mother liquid; adding the beta-carotene mother liquid and the lycopene mother liquid; and standing and refrigerating, and adding the rosemary extract mother liquid. The storage capacity of the paeonia ostii seed oil is greatly improved by compounding four natural products, so that the paeonia ostii seed oil has the oxidative stability, and the quality guarantee period of the paeonia ostii seed oil is prolonged.
Description
Technical field
The present invention relates to red seed oil of a kind of phoenix and preparation method thereof, particularly relate to red seed oil of a kind of oxidation stability phoenix and preparation method thereof.
Background technology
Feng Dan (Paeoniaostii T.Hong et J.X.Zhang), i.e. Tongling tree peony, former plant is Paeonia ostii.Because mainly originating in Nanling, Anhui Province Western Hills and Feng huangshan Mountain, Tongling one band, therefore being referred to as Feng Dan, belonging to Ranunculaceae Paeonia shrub.Because of the condition such as weather, soil of its uniqueness, the tree peony that is produced from Tongling has characteristic.Its flower mostly is single-lobe, in white, to have pink, and flower great Ye is emerald green, and fragrance attacks people; Especially its rhizome is round and sturdy, and the thin meat of skin is thick, and storage is never degenerated for a long time; Section mealiness foot; Color of the leather is golden yellow, and have bright star point, gloss is as copper.Within 2006, Feng Dan is listed in " national geography sign protection product ".
The red seed oil of phoenix is a kind of edible oil extract from the red seed of phoenix.Research shows, containing the unrighted acid of 89.00% in the red seed oil of phoenix, wherein main based on linoleic acid (33.34%) and leukotrienes (54.81%).The red seed of phoenix is oily in storage, processing and the process of circulation, because being subject to O in air
2, metal ion, the factor effect very easily oxidative rancidity such as illumination, cause variable color or spoiled and harmful substance can be generated, be unfavorable for the utilization of the red seed oil of phoenix.
At present, in grease, add antioxidant become in fats and oils processing the most economical and simple method preventing Oxidation of Fat and Oils rotten.Although the antioxidant action effect of Prof. Du Yucang is remarkable, because safety problem is used by the restriction of multiple countries.
In recent years, increasing natural is developed.Natural can from animals and plants extracting directly, security is high, can not only prevent Oxidation of Fat and Oils, also can prevent the harm of people's interior free yl, and delay senility the various degenerative disorders caused, as cancer, diabetes etc.
In general, synergistic function between composite antioxidant can strengthen the antioxygenic property of antioxidant, its effect is better than single antioxidant, but, while there is synergistic function, certain homogeneity, antagonistic effect is also there is between different composite antioxidant, namely compound use can not strengthen antioxidant effect, or the enhancing rate of antioxidant effect and cost are lacked of proper care, and do not have business use value.This situation is especially when several or multiple antioxidant compound use, and along with increasing of antioxidant kind, its antioxidant effect no longer includes lifting.The mechanism of action of different antioxidant has difference, and the link stopped in Oxidation of Fat and Oils process is different, and have the impact of the factors such as antagonism between composite antioxidant, composite antioxidant needs repeatedly proportioning can realize the lifting of antioxidant effect.
Summary of the invention
The invention provides the red seed oil of a kind of oxidation stability phoenix, this phoenix red seed oil specifically with the addition of citric acid and 3 kinds of naturals---beta carotene, lycopene, Rosmarinus officinalis extract in the red seed oil of the fresh phoenix obtained by Extraction solvent.The antioxygenic property of the red seed oil of phoenix can be significantly improved.And all additives are the food additives allowing in GB to add, and addition also meets the relevant regulations of GB.Wherein No. CAS of beta carotene is: 7235-40-7, and No. CAS of lycopene is: 502-65-8, and No. CAS of Rosmarinus officinalis extract is: 84604-14-8, and No. CAS of citric acid is: 77-92-9.
The red seed oil of oxidation stability phoenix, comprising mass fraction is 0.02%-0.06% beta carotene, 0.03%-0.06% lycopene, 0.03%-0.07% Rosmarinus officinalis extract and 0.01-0.10% citric acid, and surplus is the red seed oil of phoenix.
The red seed oil of oxidation stability phoenix, formula is preferably made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, and surplus is the red seed oil of phoenix.
Another object of the present invention is to provide the preparation method of the red seed oil of above-mentioned oxidation stability phoenix, and described method is simple, the red seed oil oxidation stability of phoenix promotes to some extent than commonsense method, is easy to suitability for industrialized production.
A preparation method for the red seed oil of stability phoenix, comprises following detailed step:
(1) extraction of the red seed oil of phoenix: pluck the red seed of phoenix, pulverizes dry phoenix pellet seed, and employing tradition leaches or the oily red seed oil of method extraction phoenix is carried in squeezing;
(2) additive mother liquor is prepared: the Rosmarinus officinalis extract mother liquor preparing citric acid mother liquor, the beta carotene mother liquor of 0.5%-5%, the lycopene mother liquor of 0.5%-5% and the 0.5%-5% that mass fraction is 0.5%-5% respectively with the red seed oil of phoenix;
(3) add citric acid mother liquor: take out citric acid mother liquor, join in the red seed oil of phoenix, concussion 10-30min, obtains mixture I;
(4) add beta carotene and lycopene mother liquor: take out beta carotene mother liquor and lycopene mother liquor respectively, join in mixture I, concussion 10-30min, obtains mixture II;
(5) refrigeration is left standstill: mixture II is left standstill refrigeration 24-48h;
(6) Rosmarinus officinalis extract mother liquor is added: get Rosmarinus officinalis extract mother liquor and join in the mixture II after leaving standstill, concussion 10-30min, obtains the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix.
Beneficial effect of the present invention
The beneficial effect of the red seed oil of oxidation stability phoenix
Research finds, in the red seed oil of phoenix, saturated fatty acid accounts for 10.77%, unrighted acid accounts for 89.00%.Wherein main fatty acid is leukotrienes 54.81%, linoleic acid 33.34%, palmitic acid 8.07%, stearic acid 2.29%.The oxidization time of different aliphatic acid is different with speed, and the antioxidant of different antioxidation mechanism also also exists very large difference to the antioxidant effect of different aliphatic acid.The aliphatic acid of the red seed oil of phoenix is mainly linolenic and linoleic, is very easily oxidized, forms rancidity beastly, affect the quality of product, the health of harm user in grease lixiviate and storage.
The current method of Oxidation of Fat and Oils that prevents adds antioxidant, but in recent years, people conduct extensive research for the safety issue of Prof. Du Yucang antioxidant.The World Health Organization (FA0/WHO), European Community's protection of the child tissue (HACSG), biological industry association of Britain (BIBRA), Japan, the government of the U.S. and the research of tissue show; synthetized oxidation preventive agent has many side effects; as the liver to human body, spleen, lung all have adverse effect, malignant tumour etc. can be brought out.So FDA (Food and Drug Adminstration) (FDA) suggestion leaves out BHT it is generally acknowledged in safe material; Japan once made the conclusion prohibitting the use BHA after deliberation about hygiene department; For TBHQ, although a few countries such as the U.S. have approved its use in some grease, European Union, Japan and other countries think its toxicological test data imperfection, not yet ratify to use.In a word, there is uneasy psychology to Prof. Du Yucang antioxidant in people.
The red seed oil of a kind of stability phoenix provided by the invention, have employed several natural anti-oxidation extract composite, obtain the red seed oil mixture of a kind of phoenix containing several natural anti-oxidation extract, this mixture according to the fatty acid component of the red seed oil of phoenix and oxidation characteristic formulated.
First, citric acid is added.Citric acid effectively can remove the Oxidation of Fat and Oils that metal ion causes, metal ion containing trace in the red seed oil of phoenix, these metal ions one are derived from metallic activating enzymes or its catabolite, and two is be derived from contacting metal container in phoenix red seed oil refining and food processing.Particularly those have two valencys or more high-valence state and have the metal of suitable redox potential between which to heavy metal, can shorten the time of chain reaction induction period, accelerate the speed of lipoid substance oxidation.Existing research is verified, when the porphyrin ring complex concentration of metal and slaine is 10-8mol/L, then produces Strong oxdiative effect.Some complexing agents containing oxygen coordination atom can be used as antioxidant, and because reducing redox potential behind they and complexing of metal ion, the oxidation state of stable metal ion, suppresses the enzymatic oxidation effect of metal ion effectively.After adding citric acid, eliminate the oxidative factors such as the metal ion in the red seed oil of phoenix, make beta carotene and lycopene antioxidant Be very effective.
Secondly, add beta carotene and lycopene, the i.e. compound of two kinds of materials of carotenoids, these two kinds of materials add according to the ratio of contained linoleic acid plus linolenic acid in the red seed of phoenix.Excited oxygen buries in oblivion agent simple substance oxygen has two kinds to there is energy state, and one is singlet, i.e. excitation state (1O2); Another is triplet state (3O2), i.e. ground state.Lipoid substance can be oxidized to hydroperoxides by singlet and excited oxygen, is one of reason of Oxidation of Fat and Oils.Singlet oxygen (excitation state) can be changed into triplet state (ground state) oxygen by carotenes under hypoxic stress, eliminates the existence of singlet oxygen, thus plays antioxidation.Simultaneously; lycopene is as powerful antioxidant; energy quenching singlet oxygen and scavenging free radicals; can in available protecting cell various large biological molecule by the damage of free radical; lycopene also has the ability stoping ultra violet radiation simultaneously; prevent illumination on the impact of phoenix red seed oil oxidation, stop the red seed oil of phoenix, in storage, photosensitized oxidation occurs.
Finally, add Rosmarinus officinalis extract, No. CAS is: 84604-14-8.Domestic and foreign literature data shows, the main terpene of the antioxidant content in Rosmarinus officinalis extract, phenols and acid.Due to containing multiple anti-oxidant active ingredient and multiple antioxidation mechanism, rosemary is caused to have efficient and non-oxidizability widely.Generally believe at present, Rosemary antioxidant mechanism is mainly its energy quencher creating singlet oxygen, and scavenging free radicals, cuts off the chain reaction of lipoid autoxidation, chelated metal ions etc.In Rosmarinic acid, the composition of reproducibility is as phenolic hydroxyl group, unsaturated double-bond and acid etc., has antioxidation, have synergy when combining during individualism.As can be seen here, the antioxidant effect of Rosmarinus officinalis extract is fine, there is diversity, but, the price of rosemary is higher, add if start, antioxidant content in rosemary is together with citric acid, beta carotene removes the oxidative factors in whole grease system together with lycopene, cause the inefficacy of rosemary self polyphenoils, under the condition of equal addition, the antioxidant effect of Rosmarinus officinalis extract can significantly reduce, the present invention is by adding citric acid, 24-48h is left standstill after beta carotene and lycopene, citric acid and beta carotene and lycopene are played one's part to the full, make price lower, widely used citric acid consumes when removing oxidative factors in the red seed oil systems of phoenix, and then it is better to add effect successively, the antioxidant content that price is more expensive, give full play to the effect of each antioxidant content, use while avoiding the antioxidant of same mechanism, the action effect of antioxidant is added under the condition that addition is constant, enhance the red seed oil oxidation stability of phoenix.
By accelerating contrast test, in embodiment 1, embodiment group Acceleration study under the condition of 63 ± 1 DEG C, after depositing 18 days, its peroxide value is only about 15% of red seed oil group (control group 12) of phoenix, as can be seen here, the keeping quality of the red seed oil mixture of this phoenix improves nearly 7 times, meanwhile, according to other control groups than the red seed oil of phoenix, can find out, V
c, V
eas replacement, or lack lycopene or beta carotene the red seed oil oxidation stability of phoenix all can be made to reduce, storage time also reduces accordingly.
The beneficial effect of the red seed oil method of preparation oxidation stability phoenix
The invention provides and a kind ofly improve the method that natural works in coordination with efficiency, the method in turn includes the following steps: extract the red seed oil of phoenix, the preparation of additive mother liquor, add beta carotene and lycopene, standing mixt, add Rosmarinus officinalis extract, prepare the red seed oil of phoenix with oxidation stability of the present invention by this method, its oxidation stability can be made further to be promoted, by embodiment group in embodiment 1 and control group 1, the experimental data of control group 11 can be found out, the peroxide value of acceleration after 18 days of embodiment group is starkly lower than control group 1 and control group 11.
This mixture of phoenix red seed oil with oxidation stability provided by the invention does not change the red seed original composition of oil of phoenix and color, smell and taste, all additives all belong to the food additives of national regulation, and addition meets national regulation, effectively can extend the shelf-life of the red seed oil of phoenix, solve the problem of easily oxidation deterioration in the processing of the red seed oil product of phoenix and storage, for the red seed oil product of phoenix enters the help that consumers in general market provides useful.
Preparation method of the present invention is simple, and cost is lower, is easy to suitability for industrialized production, and the red seed oil of the phoenix of being prepared by the method has good oxidation stability.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of preparation method of the present invention.
Detailed description of the invention
Embodiment 1
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, and surplus is the red seed oil of phoenix.
Wherein the red seed of phoenix originates from Tongling, Anhui Province Feng huangshan Mountain, and No. CAS of beta carotene is: 7235-40-7, and No. CAS of lycopene is: 502-65-8, and No. CAS of Rosmarinus officinalis extract is: 84604-14-8, and No. CAS of citric acid is: 77-92-9.
A preparation method for the red seed oil of stability phoenix, comprises the steps:
1. pluck the red seed of phoenix: the red seed of phoenix choosing full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. pulverize dry: red for clean phoenix seed is put into Chinese herbal medicine atomizer and pulverizes, cross 40 mesh sieves, drying obtains the red powder of seeds of phoenix.
3. extract the red seed oil of phoenix, adopt benzinum lixiviation process to extract the red seed oil of phoenix.
4. separating and filtering: after having extracted, with the mixed liquor of the red powder of seeds of Filter paper filtering phoenix with benzinum, filter paper lower floor obtains benzinum filtrate, and filter paper upper strata obtains the phoenix pellet powder of seeds of degreasing.
5. drying under reduced pressure: adopt Rotary Evaporators to be 50kPa in vacuum, under the condition that temperature is 40 DEG C, rotary evaporation also reclaims benzinum, until benzinum is distilled out of completely, obtains the red seed oil of phoenix.
6. in four brown bottles, accurately take the red seed oil of 9.9000g phoenix respectively, stand-by.
7. the preparation of additive mother liquor: accurately take citric acid, beta carotene, lycopene, each 0.1000g of Rosmarinus officinalis extract with assay balance, to join in step 6 in four brown bottles containing the red seed oil of 9.9000g phoenix respectively successively, and seal, putting into 20 DEG C of water-baths concussion case lucifuges concussion 30min makes it be uniformly dispersed, be made into respectively mass fraction be 1% citric acid mother liquor, the beta carotene mother liquor of 1%, the lycopene mother liquor of 1% and 1% Rosmarinus officinalis extract mother liquor.Under being stored in 4 DEG C of air-proof conditions, for subsequent use.
8. add citric acid mother liquor: take out 2mL1% citric acid mother liquor, join in the red seed oil of 89mL phoenix, 20 DEG C of Water Under bath concussion 10min, make it be uniformly dispersed, obtain the mixture of citric acid and the red seed oil of phoenix, called after mixture I.
9. add beta carotene and lycopene mother liquor: take out the beta carotene mother liquor of 2mL1% and the lycopene mother liquor of 3mL1% respectively, join in the mixture I that step 8 obtains, 20 DEG C of Water Under bath concussion 30min, make it be uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
10. standing mixt II: mixture II is refrigerated 24h under the condition of 4 DEG C.
11. add Rosmarinus officinalis extract mother liquor: get 4mL1% Rosmarinus officinalis extract mother liquor respectively and join in the mixture II after leaving standstill, 20 DEG C of Water Under bath concussion 30min, make it be uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, for the present invention obtains having the red seed oil of phoenix of stability.
Product checking and contrast experiment:
Embodiment group: according to the step in the present embodiment, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, and surplus is the red seed oil of phoenix.By this group called after embodiment group.
Control group 1: remove the step 10 in the present embodiment group, other steps are with embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, and surplus is the red seed oil of phoenix.By this group called after control group 1.
Control group 2: by the step of embodiment group, the part of contained Rosmarinus officinalis extract changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.02% citric acid, and surplus is the red seed oil of phoenix.By this group called after control group 2.
Control group 3: by the step of embodiment group, the part of contained beta carotene and lycopene changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture forms Rosmarinus officinalis extract and 0.02% citric acid of 0.04% by the material of following mass percent, and surplus is the red seed oil of phoenix.By this group called after control group 3.
Control group 4: by the step of embodiment group, the part of contained citric acid changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.04% Rosmarinus officinalis extract, surplus is the red seed oil of phoenix.By this group called after control group 4.
Control group 5: by the step of embodiment group, the part of contained citric acid, lycopene and beta carotene changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: the Rosmarinus officinalis extract of 0.04%, and surplus is the red seed oil of phoenix.By this group called after control group 5.
Control group 6: by the step of embodiment group, the part of contained beta carotene changes the lycopene of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.05% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after control group 6.
Control group 7: by the step of embodiment group, the part of contained lycopene changes the beta carotene of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.05% beta carotene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after control group 7.
Control group 8: by the step of embodiment group, the part of contained beta carotene, lycopene changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: the Rosmarinus officinalis extract of 0.04% and 0.02% citric acid, and surplus is the red seed oil of phoenix.By this group called after control group 8.
Control group 9: by the step of embodiment group, the part of contained citric acid changes the vitamin C of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.04% Rosmarinus officinalis extract and 0.02% vitamin C, surplus is the red seed oil of phoenix.By this group called after control group 9.
Control group 10: by the step of embodiment group, the part of contained Rosmarinus officinalis extract changes the vitamin E of equivalent weight into, other are with the step in embodiment group, prepare the mixture of the red seed oil of 2 parts of 100mL phoenix, this mixture is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.04% Rosmarinus officinalis extract and 0.02% vitamin E, surplus is the red seed oil of phoenix.By this group called after control group 10.
Control group 11: by the step of embodiment group, the beta carotene mother liquor of 2mL1% citric acid mother liquor, 2mL1%, the lycopene mother liquor of 3mL1% join in the red seed oil of 89mL phoenix together with 4mL1% Rosmarinus officinalis extract mother liquor, 20 DEG C of Water Under bath concussion 10min, make it be uniformly dispersed, obtain the mixture of citric acid and the red seed oil of phoenix, this mixture is made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix, by this group called after control group 11.
Control group 12: get undressed 100mL phoenix red seed oil called after control group 12.
By the uncovered digital display thermostatic drying chamber being placed in 63 ± 1 DEG C of the red seed oil mixture of the phoenix of embodiment group and all control groups, every 2 days sampling analysis measuring peroxide values, observe the quality comparison of sample under 63 ± 1 DEG C of environment.The assay method of peroxide value performs by with reference to GB/T5538-2005.
The situation of change of its peroxide value is as shown in table 1, and abscissa is the mean value of each group of peroxide value, and ordinate is the number of days under 63 ± 1 DEG C of environment.(peroxide value unit meq/kg)
Table 1, the peroxide value Changing Pattern of embodiment group and control group
Table 1 continues, the peroxide value Changing Pattern of embodiment group and control group
As can be seen from the data in form, adopt the method in embodiment 1 and addition in experimental period, effectively can delay the rising of phoenix red seed oil peroxide value, embodiment 1 group is significantly better than other groups.By change adding method or change other naturals antioxidant effect obviously than difference of the present invention.The red seed oil of the phoenix obtained by method of the present invention can under the condition of 63 ± 1 DEG C, and effectively control the oxidation of the red seed of phoenix in 20 days, its peroxide value of 20 days is only 13.4311meq/kg, greatly improves the keeping quality of the red seed oil of phoenix.
Embodiment 2
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.03% beta carotene, 0.04% lycopene, 0.04% Rosmarinus officinalis extract and 0.07% citric acid, and surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. pluck the red seed of phoenix: the red seed of phoenix choosing full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. pulverize dry: red for clean phoenix seed is put into Chinese herbal medicine atomizer and pulverizes, cross 100 mesh sieves, drying obtains the red powder of seeds of phoenix.
3. extract the red seed oil of phoenix, adopt lixiviation process to extract the red seed oil of phoenix.
4. separating and filtering: after having extracted, with the mixed liquor of the red powder of seeds of Filter paper filtering phoenix with benzinum, filter paper lower floor obtains benzinum filtrate, and filter paper upper strata obtains the phoenix pellet powder of seeds of degreasing.
5. drying under reduced pressure: adopt Rotary Evaporators to be 50kPa in vacuum, under the condition that temperature is 40 DEG C, rotary evaporation also reclaims benzinum, until benzinum is distilled out of completely, obtains the red seed oil of phoenix.
6. in four brown bottles, accurately take the red seed oil of 9.9000g phoenix respectively, stand-by.
7. the preparation of additive mother liquor: accurately take citric acid, beta carotene, lycopene, each 0.0500g of Rosmarinus officinalis extract with assay balance, to join in step 6 in four brown bottles containing the red seed oil of 9.9500g phoenix respectively successively, and seal, putting into 20 DEG C of water-baths concussion case lucifuges concussion 10min makes it be uniformly dispersed, be made into respectively mass fraction be 0.5% citric acid mother liquor, the beta carotene mother liquor of 0.5%, the lycopene mother liquor of 0.5% and 0.5% Rosmarinus officinalis extract mother liquor.Under being stored in 0 DEG C of air-proof condition, for subsequent use.
8. add citric acid mother liquor: take out 14mL1% citric acid mother liquor, join in the red seed oil of 64mL phoenix, 20 DEG C of Water Under bath concussion 10min, make it be uniformly dispersed, obtain the mixture of citric acid and the red seed oil of phoenix, called after mixture I.
9. add beta carotene and lycopene mother liquor: take out the beta carotene mother liquor of 6mL0.5% and the lycopene mother liquor of 8mL0.5% respectively, join in the mixture I that step 8 obtains, 4 DEG C of Water Under bath concussion 10min, make it be uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
10. standing mixt II: mixture II is refrigerated 48h under the condition of 4 DEG C.
11. add Rosmarinus officinalis extract mother liquor: get 8mL0.5% Rosmarinus officinalis extract mother liquor respectively and join in the mixture II after leaving standstill, 20 DEG C of Water Under bath concussion 10min, make it be uniformly dispersed, obtaining the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, is the red seed oil of the phoenix with oxidation stability that the present invention obtains.
Embodiment 3
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.03% beta carotene, 0.04% lycopene, 0.03% Rosmarinus officinalis extract and 0.10% citric acid, and surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. pluck the red seed of phoenix: the red seed of phoenix choosing full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. squeezing is fuel-displaced: adopt the fuel-displaced equipment of squeezing to the red seed squeezing of phoenix, produce the red seed oil of phoenix.
3. in four brown bottles, accurately take the red seed oil of 9.9000g phoenix respectively, stand-by.
The preparation of 4 additive mother liquors: accurately take citric acid, beta carotene, lycopene, each 0.5000g of Rosmarinus officinalis extract with assay balance, to join in step 3 in four brown bottles containing the red seed oil of 9.5000g phoenix respectively successively, and seal, putting into 20 DEG C of water-baths concussion case lucifuges concussion 10min makes it be uniformly dispersed, be made into respectively mass fraction be 5% citric acid mother liquor, the beta carotene mother liquor of 5%, the lycopene mother liquor of 5% and 5% Rosmarinus officinalis extract mother liquor.Under being stored in 4 DEG C of air-proof conditions, for subsequent use.
5. add citric acid mother liquor: take out 2mL5% citric acid mother liquor, join in the red seed oil of 96mL phoenix, 20 DEG C of Water Under bath concussion 20min, make it be uniformly dispersed, obtain the mixture of citric acid and the red seed oil of phoenix, called after mixture I.
6. add beta carotene and lycopene mother liquor: take out the beta carotene mother liquor of 0.6mL5% and the lycopene mother liquor of 0.8mL5% respectively, join in the mixture I that step 5 obtains, 20 DEG C of Water Under bath concussion 30min, make it be uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
7. standing mixt II: mixture II is refrigerated 48h under the condition of 4 DEG C.
8. add Rosmarinus officinalis extract mother liquor: get 0.6mL5% Rosmarinus officinalis extract mother liquor respectively and join in the mixture II after leaving standstill, 20 DEG C of Water Under bath concussion 20min, make it be uniformly dispersed, obtaining the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, is the red seed oil of the phoenix with oxidation stability that the present invention obtains.
Embodiment 4
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.06% beta carotene, 0.06% lycopene, 0.07% Rosmarinus officinalis extract and 0.05% citric acid, and surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. pluck the red seed of phoenix: the red seed of phoenix choosing full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. pulverize dry: red for clean phoenix seed is put into Chinese herbal medicine atomizer and pulverizes, cross 100 mesh sieves, drying obtains the red powder of seeds of phoenix.
3. extract the red seed oil of phoenix, adopt lixiviation process to extract the red seed oil of phoenix.
4. separating and filtering: after having extracted, with the mixed liquor of the red powder of seeds of Filter paper filtering phoenix with benzinum, filter paper lower floor obtains benzinum filtrate, and filter paper upper strata obtains the phoenix pellet powder of seeds of degreasing.
5. drying under reduced pressure: adopt Rotary Evaporators to be 50kPa in vacuum, under the condition that temperature is 40 DEG C, rotary evaporation also reclaims benzinum, until benzinum is distilled out of completely, obtains the red seed oil of phoenix.
6. in four brown bottles, accurately take the red seed oil of 9.9000g phoenix respectively, stand-by.
7. the preparation of additive mother liquor: accurately take citric acid, beta carotene, lycopene, each 0.1000g of Rosmarinus officinalis extract with assay balance, to join in step 6 in four brown bottles containing the red seed oil of 9.9000g phoenix respectively successively, and seal, putting into 20 DEG C of water-baths concussion case lucifuges concussion 10min makes it be uniformly dispersed, be made into respectively mass fraction be 1% citric acid mother liquor, the beta carotene mother liquor of 1%, the lycopene mother liquor of 1% and 1% Rosmarinus officinalis extract mother liquor.Under being stored in 0 DEG C of air-proof condition, for subsequent use.
8. add citric acid mother liquor: take out 5mL1% citric acid mother liquor, join in the red seed oil of 76mL phoenix, 20 DEG C of Water Under bath concussion 10min, make it be uniformly dispersed, obtain the mixture of citric acid and the red seed oil of phoenix, called after mixture I.
9. add beta carotene and lycopene mother liquor: take out the beta carotene mother liquor of 6mL1% and the lycopene mother liquor of 6mL1% respectively, join in the mixture I that step 8 obtains, 15 DEG C of Water Under bath concussion 20min, make it be uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
10. standing mixt II: mixture II is refrigerated 36h under the condition of 4 DEG C.
11. add Rosmarinus officinalis extract mother liquor: get 7mL1% Rosmarinus officinalis extract mother liquor respectively and join in the mixture II after leaving standstill, 15 DEG C of Water Under bath concussion 20min, make it be uniformly dispersed, obtaining the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, is the red seed oil of the phoenix with oxidation stability that the present invention obtains.
Embodiment 5
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.04% beta carotene, 0.03% lycopene, 0.07% Rosmarinus officinalis extract and 0.03% citric acid, and surplus is the red seed oil of phoenix.
Except addition makes corresponding change, preparation method is with embodiment 1.
Embodiment 6
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.02% beta carotene, 0.04% lycopene, 0.05% Rosmarinus officinalis extract and 0.01% citric acid, and surplus is the red seed oil of phoenix.
Except addition makes corresponding change, preparation method is with embodiment 1.
Embodiment 7
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.06% beta carotene, 0.06% lycopene, 0.07% Rosmarinus officinalis extract and 0.05% citric acid, and surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. extract phoenix red seed oil: adopt milling process to extract the red seed oil of phoenix.
2. prepare four kinds of additive mother liquors: be made into respectively mass fraction be 1% citric acid mother liquor, the beta carotene mother liquor of 1%, the lycopene mother liquor of 1% and 1% Rosmarinus officinalis extract mother liquor.Under being stored in 0 DEG C of air-proof condition, for subsequent use.
3. add citric acid mother liquor: citric acid mother liquor is added in the red seed oil of phoenix, is uniformly dispersed, makes the final concentration 0.05% of citric acid.
4. add beta carotene and lycopene mother liquor: beta carotene and lycopene mother liquor are added in the red seed oil of phoenix, is uniformly dispersed, makes final concentration be beta carotene 0.06%, lycopene 0.06%.
5. leave standstill refrigeration: mixture step 4 obtained leaves standstill refrigeration 48h under the condition of 0 DEG C.
6. add Rosmarinus officinalis extract mother liquor: Rosmarinus officinalis extract mother liquor is added in the red seed oil of phoenix, is uniformly dispersed, makes the final concentration 0.05% of Rosmarinus officinalis extract.Finally obtaining the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, is the red seed oil of the phoenix with the stability finished product that the present invention obtains.
Embodiment 8
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.03% beta carotene, 0.02% lycopene, 0.07% Rosmarinus officinalis extract and 0.04% citric acid, and surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. extract phoenix red seed oil: adopt No. six extraction methods to extract the red seed oil of phoenix.
2. prepare four kinds of additive mother liquors: be made into respectively mass fraction be 5% citric acid mother liquor, the beta carotene mother liquor of 5%, the lycopene mother liquor of 5% and 5% Rosmarinus officinalis extract mother liquor.Under being stored in 0 DEG C of air-proof condition, for subsequent use.
3. add citric acid mother liquor: citric acid mother liquor is added in the red seed oil of phoenix, is uniformly dispersed, makes the final concentration 0.04% of citric acid
4. add beta carotene and lycopene mother liquor: beta carotene and lycopene mother liquor are added in the red seed oil of phoenix, is uniformly dispersed, makes final concentration be beta carotene 0.03%, lycopene 0.02%.
5. leave standstill refrigeration: mixture step 4 obtained leaves standstill refrigeration 24h under the condition of 0 DEG C.
6. add Rosmarinus officinalis extract mother liquor: Rosmarinus officinalis extract mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, making the final concentration 0.07% of Rosmarinus officinalis extract finally obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, is the red seed oil of the phoenix with the stability finished product that the present invention obtains.
Embodiment 9
The red seed oil of stability phoenix, is made up of the material of following mass percent: 0.03% beta carotene, 0.02% lycopene, 0.03% Rosmarinus officinalis extract and 0.03% citric acid, and surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. extract phoenix red seed oil: adopt hydraulic oil press to extract the red seed oil of phoenix.
2. prepare four kinds of additive mother liquors: be made into respectively mass fraction be 0.5% citric acid mother liquor, the beta carotene mother liquor of 0.5%, the lycopene mother liquor of 0.5% and 0.5% Rosmarinus officinalis extract mother liquor.Under being stored in 4 DEG C of air-proof conditions, for subsequent use.
3. add citric acid mother liquor: citric acid mother liquor is added in the red seed oil of phoenix, is uniformly dispersed, makes the final concentration of citric acid be 0.03%.
4. add beta carotene and lycopene mother liquor: beta carotene and lycopene mother liquor are added in the red seed oil of phoenix, is uniformly dispersed, makes final concentration be beta carotene 0.03%, lycopene 0.02%.
5. leave standstill refrigeration: mixture step 4 obtained leaves standstill refrigeration 24h under the condition of 0 DEG C.
6. add Rosmarinus officinalis extract mother liquor: Rosmarinus officinalis extract mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, making the final concentration 0.05% of Rosmarinus officinalis extract finally obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, is the red seed oil of the phoenix with the stability finished product that the present invention obtains.
Claims (5)
1. the red seed oil of oxidation stability phoenix, it is characterized in that: described in there is oxidation stability the red seed oil of phoenix be made up of the material of following mass percent: 0.02%-0.06% beta carotene, 0.03%-0.06% lycopene, 0.03%-0.07% Rosmarinus officinalis extract and 0.01%-0.10% citric acid, surplus is the red seed oil of phoenix, and wherein No. CAS of Rosmarinus officinalis extract is 84604-14-8.
2. the red seed oil of oxidation stability phoenix according to claim 1, it is characterized in that: described in there is oxidation stability the red seed oil of phoenix be made up of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.
3. oxidation stability phoenix according to claim 1 red seed oil preparation method, is characterized in that: described preparation comprises step successively: the Rosmarinus officinalis extract mother liquor preparing citric acid mother liquor, the beta carotene mother liquor of 0.5%-5%, the lycopene mother liquor of 0.5%-5% and the 0.5%-5% that mass fraction is 0.5%-5% respectively with the red seed oil of phoenix; Add citric acid mother liquor; Add beta carotene and lycopene mother liquor; Leave standstill the mixture 24-48h containing beta carotene, lycopene and citric acid; Add Rosmarinus officinalis extract mother liquor.
4. the preparation method of the red seed oil of oxidation stability phoenix according to claim 3, is characterized in that: described preparation method comprises step:
(1) extract phoenix red seed oil: adopt the grease in the red seed of lixiviation process extraction phoenix, obtain the red seed oil of phoenix;
(2) four kinds of additive mother liquors are prepared: the Rosmarinus officinalis extract mother liquor preparing citric acid mother liquor, the beta carotene mother liquor of 0.5%-5%, the lycopene mother liquor of 0.5%-5% and the 0.5%-5% that mass fraction is 0.5%-5% respectively with the red seed oil of phoenix;
(3) add citric acid mother liquor: take out citric acid mother liquor, join in the red seed oil of phoenix, be uniformly dispersed, obtain mixture I;
(4) beta carotene and lycopene mother liquor is added: take out beta carotene mother liquor and lycopene mother liquor respectively, join in mixture I, be uniformly dispersed, obtain mixture II;
(5) refrigeration is left standstill: mixture II is refrigerated 24-48h;
(6) Rosmarinus officinalis extract mother liquor is added: get Rosmarinus officinalis extract mother liquor and join in the mixture II after leaving standstill refrigeration, be uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix.
5. the preparation method of the red seed oil of oxidation stability phoenix according to claim 4, is characterized in that: described employing lixiviation process extracts the grease in the red seed of phoenix or is milling process.
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