CN103598564A - Method for brewing soy sauce by cottonseed cakes - Google Patents
Method for brewing soy sauce by cottonseed cakes Download PDFInfo
- Publication number
- CN103598564A CN103598564A CN201310564614.8A CN201310564614A CN103598564A CN 103598564 A CN103598564 A CN 103598564A CN 201310564614 A CN201310564614 A CN 201310564614A CN 103598564 A CN103598564 A CN 103598564A
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- Prior art keywords
- bacterium
- soy sauce
- gossypol
- cottonseed cake
- degraded
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/50—Soya sauce
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for brewing soy sauce by cottonseed cakes. The method is characterized in that a gossypol degrading strain is used for degrading free gossypol in the cottonseed cakes; the treated cottonseed cakes are used as raw materials for brewing the soy sauce, wherein the degrading efficiency is up to more than 90%.
Description
Technical field
The method that a kind of cottonseed cake of the present invention makes soy sauce.
Background technology
In China's cotton cake dregs, protein content accounts for 36.3% ~ 47.0%, is only second to dregs of beans, with rice, compares with wheat, and protein content exceeds 5 ~ 8 times.After cotton cake dregs hydrolysis, can obtain l7 seed amino acid, wherein lysine content is lower, be 1.40% ~ 2.13%, and arginine content is higher, is 3.94% ~ 4.98%, lysine with the ratio of arginine considerably beyond the ideal value of 100: 120.From 8 kinds of essential amino acids, cottonseed protein and soybean protein are more approaching, and from vitamin and mineral matter aspect, cotton cake dregs contains abundant vitamin E and B family vitamin etc., and phosphorus content is more than 0.83% ~ 1.04%, and calcium is lower than 0.03%.Therefore, cotton cake dregs is one of important vegetable protein resource.
Gossypol is a kind of toxic component, by the form of its existence, can be divided into free gossypol (FGP, Free Gossypo1) and bound gossoypol (UGP, United Gossypo1), and both sums are referred to as total gossypol TGP.Bound gossoypol is that gossypol steams in stir-fry process at high temperature, the bond that protein in free gossypol and cotton benevolence, amino acid, phosphatide etc. form, lost activity, can not absorb in digested road, can with ight soil, excrete very soon, therefore nonhazardous effect, and FGP can absorb in digested road, to the toxic effect of people and animals' body.
Biological fermentation process is generally considered the safest current, detoxification efficiency good, production cost is low, the most rising poison-removing method.
Summary of the invention
A kind of method that the object of the present invention is to provide cottonseed cake to make soy sauce, is achieved through the following technical solutions:
The method that described cottonseed cake makes soy sauce, is characterized in that adopting the free gossypol in gossypol degradation bacteria strains degraded cottonseed cake, and the cottonseed cake after processing is as the raw material of soy sauce brewing; Wherein, the efficiency of described degraded reaches more than 90%.
One as technique scheme is supplemented, and wherein said degraded comprises the following steps:
A) in cottonseed cake: water: the ratio of gossypol degradation bacteria strains=1:0.4-1:0.06-0.1 is mixed;
B) in temperature, be 25 ℃-32 ℃, under the condition that pH value is 4-6, within gravity-flow ventilation 36-60 hour, cultivate.
In the process of degraded, there is following process: when 1. cottonseed cake ferments, the amino acid of part free gossypol and microorganism secretion, or the combination of reactive protein free ammonia base, form bound gossoypol; 2. the enzyme of the degradable gossypol of microorganism secretion, decomposes and utilizes free gossypol, thereby gossypol content is reduced.
Cotton cake dregs is after microorganism fermentation, and ANFs obviously reduces, and protein content is increased, and amino acid content tends to balance, and vitamin is abundant, contains some beneficial bacteriums and UGF etc., and nutritive value improves greatly.
Cottonseed cake after having degraded can directly be used in subsequent processing, or all adds or mix with other fermentation raw materials.
Same, as of technique scheme, supplement, wherein said soy sauce brewing comprises the following steps:
A) by all or part of being added to the water of cottonseed cake after processing;
B) at least two or more fermented bacterium mixed culture of inoculation, and remain on 25 ℃-32 ℃.
Same, one as technique scheme is supplemented, wherein said gossypol degradation bacteria strains is selected from one or more in following bacterial classification, comprising: the mould genus of bacterium pears spore broom (Scopulariopsis sp.), aspergillus (Aspergillus sp.), the mould genus of bacterium beading (Monilia sp.), bacterium monascus (Mon-ascus sp.), Candida tropicalis (Candida tropicalis Strain ZAU-I), endomycopsi.sp bacterium (
endomycopsis), Lactobacillus plantarum (
l.plantarum) and bacillus subtilis (Bacillus subtilis).
Advantage of the present invention is, gossypol toxicity can be effectively removed in microorganism fermentation dephenolize, and its degradation rate surpasses 90%, and does not add any chemicals, organic solvent; The protein content of microorganism fermented virus-free cottonseed grouts, generally higher than 40%, generates mycoprotein and vitamin in reduce toxicity, makes that protein and various essential amino acid are abundant, balance; Microbial fermentation technology detoxification, has that virus elimination rate is high, fermentation time is short, it is little to invest, comparison in equipment is simple, technique is simple, its technique does not have waste water pollution problem, and cost is low etc.
The specific embodiment
Embodiment 1
Employing aspergillus (
aspergillus sp.) to carry out gossypol degradable fermented, the amount of water of cultivating material is 1:0.8, and cultivation temperature is 30 ℃, and 48h is cultivated in gravity-flow ventilation.
Embodiment 2
Candida tropicalis CA61, endomycopsi.sp bacterium E02 and Lactobacillus plantarum composite fermentation are Candida tropicalis to the optimum process condition of cottonseed cake dephenolize: endomycopsi.sp bacterium: Lactobacillus plantarum proportioning is 3:2:1, the amount of water of cultivating material is 1:1, pH value is 5.0, cultivation temperature is 25 ℃, cultivates 60h.
Embodiment 3
The cultured products of embodiment 1 or embodiment 2 is mixed to inoculated aspergillus niger and aspergillus oryzae koji, heat-preservation fermentation at 30 ℃, products obtained therefrom sterilization, packing with water.
Embodiment 4
Adopt phloroglucinol method to detect the content of cottonseed cake Free Gossypol
Before fermentation, the content of cottonseed cake Free Gossypol is: 550 mg/kg are after the fermentation dephenolize of embodiment 2, and the content of cottonseed cake Free Gossypol is down to 33.18 mg/kg, and the efficiency of degraded reaches 94%.
Claims (4)
1. the method that cottonseed cake makes soy sauce, is characterized in that adopting the free gossypol in gossypol degradation bacteria strains degraded cottonseed cake, and the cottonseed cake after processing is as the raw material of soy sauce brewing; Wherein, the efficiency of described degraded reaches more than 90%.
2. method according to claim 1, wherein said degraded comprises the following steps:
In cottonseed cake: water: the ratio of gossypol degradation bacteria strains=1:0.4-1:0.06-0.1 is mixed;
In temperature, be 25 ℃-32 ℃, under the condition that pH value is 4-6, within gravity-flow ventilation 36-60 hour, cultivate.
3. method according to claim 1, wherein said soy sauce brewing comprises the following steps:
By all or part of being added to the water of cottonseed cake after processing;
Inoculate at least two or more fermented bacterium mixed cultures, and remain on 25 ℃-32 ℃.
4. method according to claim 1, wherein said gossypol degradation bacteria strains is selected from one or more in following bacterial classification, comprising: the mould genus of bacterium pears spore broom (
scopulariopsis sp.), aspergillus (
aspergillus sp.), the mould genus of bacterium beading (
monilia sp.), bacterium monascus (
mon-ascus sp.), Candida tropicalis (
candida tropicalis StrainzAU-I), endomycopsi.sp bacterium (
endomycopsis), Lactobacillus plantarum (
l.plantarum) and bacillus subtilis (
bacillus subtilis).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310564614.8A CN103598564A (en) | 2013-11-14 | 2013-11-14 | Method for brewing soy sauce by cottonseed cakes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310564614.8A CN103598564A (en) | 2013-11-14 | 2013-11-14 | Method for brewing soy sauce by cottonseed cakes |
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| Publication Number | Publication Date |
|---|---|
| CN103598564A true CN103598564A (en) | 2014-02-26 |
Family
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310564614.8A Pending CN103598564A (en) | 2013-11-14 | 2013-11-14 | Method for brewing soy sauce by cottonseed cakes |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104328063A (en) * | 2014-06-27 | 2015-02-04 | 安徽农业大学 | Gossypol degradation strain coming from ruminant rumens and application thereof |
| CN105255793A (en) * | 2015-11-20 | 2016-01-20 | 江南大学 | Lactobacillus plantarum capable of degrading gossypol and application of lactobacillus plantarum |
| CN105685490A (en) * | 2016-01-26 | 2016-06-22 | 曲阜师范大学 | Stevia rebaudiana waste residue fermented product compounded detoxified cottonseed kernel feed and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3682645A (en) * | 1970-01-20 | 1972-08-08 | Research Corp | Detoxification of gossypol by eumycophyta |
| CN1044497A (en) * | 1989-01-25 | 1990-08-08 | 中山大学 | The microbial detoxification method of cottonseed cake |
| CN1058139A (en) * | 1990-07-20 | 1992-01-29 | 华中农业大学 | The method that a kind of cottonseed cake dephenolize makes soy sauce |
| CN1079118A (en) * | 1992-05-27 | 1993-12-08 | 山东大学 | A kind of is the method that raw material makes soy sauce with cottonseed cake (dregs of rice) |
| CN101270336A (en) * | 2008-05-09 | 2008-09-24 | 石河子大学 | Candida tropicalis ZD-3 bacterial strain, uses and method for producing biological protein feedstuff thereof |
| CN102823726A (en) * | 2012-09-07 | 2012-12-19 | 北京大北农科技集团股份有限公司 | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity |
-
2013
- 2013-11-14 CN CN201310564614.8A patent/CN103598564A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3682645A (en) * | 1970-01-20 | 1972-08-08 | Research Corp | Detoxification of gossypol by eumycophyta |
| CN1044497A (en) * | 1989-01-25 | 1990-08-08 | 中山大学 | The microbial detoxification method of cottonseed cake |
| CN1058139A (en) * | 1990-07-20 | 1992-01-29 | 华中农业大学 | The method that a kind of cottonseed cake dephenolize makes soy sauce |
| CN1079118A (en) * | 1992-05-27 | 1993-12-08 | 山东大学 | A kind of is the method that raw material makes soy sauce with cottonseed cake (dregs of rice) |
| CN101270336A (en) * | 2008-05-09 | 2008-09-24 | 石河子大学 | Candida tropicalis ZD-3 bacterial strain, uses and method for producing biological protein feedstuff thereof |
| CN102823726A (en) * | 2012-09-07 | 2012-12-19 | 北京大北农科技集团股份有限公司 | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity |
Non-Patent Citations (2)
| Title |
|---|
| 李佳，等: "棉酚降解菌株的分离及对棉饼的脱毒效果", 《江苏农业科学》, vol. 40, no. 11, 31 December 2012 (2012-12-31), pages 220 - 224 * |
| 郭书贤，等: "微生物发酵棉籽饼粕脱毒与利用研究进展", 《中国酿造》, no. 1, 31 December 2009 (2009-12-31), pages 4 - 10 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104328063A (en) * | 2014-06-27 | 2015-02-04 | 安徽农业大学 | Gossypol degradation strain coming from ruminant rumens and application thereof |
| CN105255793A (en) * | 2015-11-20 | 2016-01-20 | 江南大学 | Lactobacillus plantarum capable of degrading gossypol and application of lactobacillus plantarum |
| CN105255793B (en) * | 2015-11-20 | 2019-03-01 | 江南大学 | A kind of lactobacillus plantarum and its application with degradation gossypol effect |
| CN105685490A (en) * | 2016-01-26 | 2016-06-22 | 曲阜师范大学 | Stevia rebaudiana waste residue fermented product compounded detoxified cottonseed kernel feed and preparation method thereof |
| CN105685490B (en) * | 2016-01-26 | 2020-06-30 | 曲阜师范大学 | Stevia rebaudiana waste residue fermentation product compound detoxified cottonseed kernel feed and preparation method thereof |
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Application publication date: 20140226 |