CN103566282B - A kind of Traditional Chinese medicine composition with anti-tumor effect and preparation method - Google Patents
A kind of Traditional Chinese medicine composition with anti-tumor effect and preparation method Download PDFInfo
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Abstract
The present invention relates to a kind of Traditional Chinese medicine composition with anti-tumor effect, prepared by following methods：Take 15 parts of weight portion medicinal material root tuber of aromatic turmeric, 15 parts of hairyvein agrimony, 15 parts of excrementum pteropi, 15 parts of alum, 15 parts of nitre, 0.5 3 parts of dried lacquer processed, 37 parts of stir-baked FRUCTUS AURANTII in bran, 0.5 4 parts of prepared nux vomica；The taste medicinal material of the above 8, takes root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed and crushes, and ethanol is extracted, and extract solution depressurizes to obtain extractum A, and the dregs of a decoction are standby；Take hairyvein agrimony, alum, nitre, excrementum pteropi to be added water to cook with the above-mentioned dregs of a decoction, decoction liquor filtering, filtrate concentrate drying obtains powder B；Above-mentioned extractum A, powder B and prepared nux vomica are mixed, and effect is better than former technique, and dose is low, and patient compliance is good.
Description
Technical field
The present invention relates to field of medicaments, and in particular to be a kind of Chinese medicine composition of antitumor action and preparation method thereof.
Background technology
The existing flat oral preparation for eliminating of Chinese patent drug includes tablet, capsule, is a kind of conventional antineoplastic Chinese traditional medicine.《Chinese Pharmacopoeia》
Version one page 639 records the items such as prescription, preparation method, proterties and discriminating within 2010.Specifically preparation method is：The taste medicinal material of the above eight, does
Paint, excrementum pteropi, alum, nitre are ground into fine powder；Root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran are ground into most meal, are solvent with 70% ethanol, carry out
Diacolation, collects percolate 600ml, reclaims ethanol, standby；The dregs of a decoction added water to cook with hairyvein agrimony it is secondary, filtration, merging filtrate and with
Above-mentioned percolate merges, and is concentrated under reduced pressure into thick paste, dries, and adds prepared nux vomica, above-mentioned fine powder and appropriate starch, mixes, granulation,
Dry, load capsule, be made 1000, obtain final product capsule.Or prepared nux vomica, above-mentioned fine powder and right amount of auxiliary materials are added, mix,
Granulation, dries, and is pressed into 1000, sugar coating or film-coating, obtains final product tablet.
In the prior art, the oral dose of PING XIAO JIAO NANG and Ping Xiao Pian is 4-8 tablets/time or 4-8 pieces/times, the load medicine of preparation
Than relatively low, the compliance of patient is weaker, so as to affect the treatment for amount.
The content of the invention
In order to solve the above-mentioned technical problem, the invention provides a kind of curative effect stabilization, drugloading rate Chinese medicine composition high and
Preparation method.
It is a still further object of the present invention to provide the pharmaceutically acceptable preparation containing said composition.
The present invention is achieved by the following technical solutions.
A kind of Chinese medicine composition of antitumor action of the present invention, is prepared by following step：
(1) take 1-5 parts of weight portion medicinal material root tuber of aromatic turmeric, hairyvein agrimony 1-5 parts, excrementum pteropi 1-5 parts, alum 1-5 parts, nitre 1-5 parts,
Dried lacquer 0.5-3 parts processed, stir-baked FRUCTUS AURANTII in bran 3-7 parts, prepared nux vomica 0.5-4 parts；
(2) 8 taste medicinal material more than, takes root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed and crushes, and ethanol is extracted, and extract solution depressurizes to obtain extractum A,
The dregs of a decoction are standby；
(3) take hairyvein agrimony, alum, nitre, excrementum pteropi to be added water to cook with the above-mentioned dregs of a decoction, decoction liquor filtering, filtrate concentration is dry
It is dry to obtain powder B；
(4) above-mentioned extractum A, powder B and prepared nux vomica are mixed.
Preferably, 2-4 parts of weight portion medicinal material root tuber of aromatic turmeric, hairyvein agrimony 2-4 parts, excrementum pteropi 2-4 are taken described in above-mentioned steps (1)
Part, alum 2-4 parts, nitre 2-4 parts, dried lacquer 0.5-2 parts processed, stir-baked FRUCTUS AURANTII in bran 4-6 parts, prepared nux vomica 1-3 parts.Optimally, step
(1) 3 parts of weight portion medicinal material root tuber of aromatic turmeric, 3 parts of hairyvein agrimony, 2.5 parts of excrementum pteropi, 3 parts of alum, 3 parts of nitre, dried lacquer processed 1 are taken described in
Part, 5 parts of stir-baked FRUCTUS AURANTII in bran, 2 parts of prepared nux vomica.
Root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed described in above-mentioned steps (2) crush alcohol extraction procedure, including but not limited to：
Refluxing extraction, ultrasonic extraction, carbon dioxide supercritical extraction, Microwave Extraction, percolation etc., preferably percolation.It is specially described
Root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed crush and use 60-80% ethanol percolations, collection percolate is recovered under reduced pressure ethanol and obtains extractum A.Most
Good crushing for described root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed uses 70% ethanol percolation, collects percolate, and ethanol is recovered under reduced pressure must be soaked
Cream A.
Percolation of the present invention is operated according to the percolation under pharmacopeia liquid extract and extract.
Preferably, take hairyvein agrimony, alum, nitre, the excrementum pteropi described in above-mentioned steps (3) are added water to cook with the above-mentioned dregs of a decoction
1-3 times, each 1-3 hours, decoction liquor filtering merges, and filtrate concentrate drying obtains powder B.
Optimally, take hairyvein agrimony, alum, nitre, the excrementum pteropi described in step (3) are added water to cook 2 times with the above-mentioned dregs of a decoction,
2 hours every time, decoction liquor filtering merged, and filtering, filtrate concentrate drying obtains powder B.
The preparation method of Traditional Chinese medicine composition with anti-tumor effect of the present invention, comprises the following steps：
(1) take 1-5 parts of weight portion medicinal material root tuber of aromatic turmeric, hairyvein agrimony 1-5 parts, excrementum pteropi 1-5 parts, alum 1-5 parts, nitre 1-5 parts,
Dried lacquer 0.5-3 parts processed, stir-baked FRUCTUS AURANTII in bran 3-7 parts, prepared nux vomica 0.5-4 parts；
(2) 8 taste medicinal material more than, takes root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed and crushes, and ethanol is extracted, and extract solution depressurizes to obtain extractum A,
The dregs of a decoction are standby；
(3) take hairyvein agrimony, alum, nitre, excrementum pteropi to be added water to cook with the above-mentioned dregs of a decoction, decoction liquor filtering, filtrate concentration is dry
It is dry to obtain powder B；
(4) above-mentioned extractum A, powder B and prepared nux vomica are mixed.
Preferably, preparation method of the present invention, comprises the following steps：
(1) take 2-4 parts of weight portion medicinal material root tuber of aromatic turmeric, hairyvein agrimony 2-4 parts, excrementum pteropi 2-4 parts, alum 2-4 parts, nitre 2-4 parts,
Dried lacquer 0.5-2 parts processed, stir-baked FRUCTUS AURANTII in bran 4-6 parts, prepared nux vomica 1-3 parts；
(2) 8 taste medicine root tubers of aromatic turmeric more than, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed are crushed and use 60-80% ethanol percolations, collect percolate, decompression
Reclaim ethanol and obtain extractum A；
(3) hairyvein agrimony, alum, nitre, excrementum pteropi is taken to be added water to cook 1-3 times with the above-mentioned dregs of a decoction, it is each 1-3 hours, decoct
Liquid filtering merges, and filtering, filtrate concentrate drying obtains powder B；
(4) above-mentioned extractum A, powder B and prepared nux vomica are mixed.
Most preferably, preparation method of the present invention, comprises the following steps：
(1) 3 parts of weight portion medicinal material root tuber of aromatic turmeric, 3 parts of hairyvein agrimony, 2.5 parts of excrementum pteropi, 3 parts of alum, 3 parts of nitre, dried lacquer processed 1 are taken
Part, 5 parts of stir-baked FRUCTUS AURANTII in bran, 2 parts of prepared nux vomica；
(2) root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed 70% ethanol percolation of crushing are taken, percolate is collected, ethanol is recovered under reduced pressure and is obtained
Extractum A；
(3) take hairyvein agrimony, alum, nitre, excrementum pteropi to be added water to cook 2 times with the above-mentioned dregs of a decoction, 2 hours every time, decoction liquor mistake
Filter merges, and filtering, filtrate concentrate drying obtains powder B；
(4) above-mentioned extractum A, powder B and prepared nux vomica are mixed.
Preparation method of the present invention, wherein step and parameter be all inventor on the basis of existing technology, protect
Hold original drug effect it is constant in the case of, for the extraction process of the creative screening of active function of each medicinal material.
Root tuber of aromatic turmeric：It is the root tuber of zingiberaceous plant turmeric, root tuber of aromatic turmeric or curcuma zedoary.According to data：Elemene energy contained in root tuber of aromatic turmeric
Direct killing cancer cell, can induce HL60Apoptosis (IC50=27.5 μ g/mL), curcumin is in 300mg/kg dosage
Under, to mouse S180Sarcoma, mouse Emhorn solid tumor have obvious inhibitory action；In testing in vitro, curcumin is to HL60Carefully
Born of the same parents, K562Cell, SGC7901Cell, Bel7402Cell is respectively provided with obvious inhibitory action, its IC50Scope is 0.56~4.15 μ g/
mL.Curcumin has many pharmacological actions, such as anti-inflammatory, anti-oxidant, reducing blood lipid and antiatherosclerosis, antitumor work
With, especially as a kind of cancer therapy drug with good development prospect, have attracted increasing attention, the heat as research
Point.In recent years, the experimental study to curcumin antitumaous effect shows that curcumin can suppress the growth of the inside and outside tumour cell of body, its
Anticancer spectrum is wider, and toxic and side effect is small, is a kind of PTS with wide application prospect.It can effectively suppress part induction
EGF-R ELISA stimulate vascular smooth muscle cell proliferation, show curcumin anti-proliferate disease such as tumour etc. just
Face has much DEVELOPMENT PROSPECT, while curcumin is carcinogenic to the Chemical Carcinogenesis such as BaP, nitrosamine, nitrosoguanidine, TPA and the mutation of cancer base
With good protective action.Someone has inquired into the oxidation resistant characteristic of curcumin from the angle of curcumin structure-activity relationship, finds ginger
Flavine contains Liang Ge p-OH groups, and this group has anti-oxidation characteristics, is also required for antimutagenic activity.Therefore curcumin is anti-
Mutagenicity may have close relationship with the inoxidizability of p-OH, and curcumin oxidation resistance is parallel with its antitumous effect
's.
Hairyvein agrimony：Hairyvein agrimony is called hairyvein agrimony, is a kind of rosaceous herbaceos perennial.The main component of its anticancer
Be agrimonine, be a kind of tannic acid, be a kind of potential antitumorigenic substance, can act on tumour cell and some immunocytes and
Strengthen the immune response of animal body.External inhibition test shows that hairyvein agrimony water-soluble extractive oozes to several tumour cells
Entering has inhibitory action, and strengthens with increasing for concentration.By intraperitoneal administration, MM can be completely inhibited2The growth of cell, and
Suppress MH134The growth of liver cancer and Meth-A fibrosarcomas.Hairyvein agrimony is whether to the tumour cell of in vitro culture, or experiment
Animal transplanting tumor has suppression or lethal effect, and can also improve tumor mice immunologic function and life cycle.Agrimophol
Also there is stronger antitumor action.It has been investigated that, agrimophol has certain killing action, in vitro test table to cancer cell
It is bright：Agrimophol just has certain killing action in 0.16mg/mL concentration to same volume ascites of liver cancer oncocyte, and
Can be in 1h by the same volume cancer cell all kills during 1.25mg/mL concentration；Lotus cancer ascites carcinoma mouse can be obviously prolonged to deposit
Live time, ascites of liver cancer cancer mouse 24, intraperitoneal injection agrimophol can significantly extend mouse survival time, increase in life span
49.6%；Agrimophol parenteral solution has good therapeutic action to animal carcasses knurl, to S37And U14Growth inhibition ratio be respectively
47.0% and 38.7 ‰ couples of S18026.3% and 23.5% are respectively with the inhibiting rate of liver cancer sarcoma.
Fructus Aurantii：Immature fruit is dried for rutaceae bitter orange Citrus aurantium L. and its variety.
Main active is Nobiletin, to the ED of nasopharyngeal carcinoma KB cells50It is 3~28 μ g/mL.In vivo to Mice Bearing Lewis Lung Cancer and
Watt gram carcinoma 256 is also effective.
Dried lacquer：It is the tree of Anacardiaceae plant lacquer tree Toxicodendron vernicifluum (Stokes) F.A.Barkl.
Dry product after fat is processed.
The 4 kinds of triterpenes tool cytotoxic effect contained in excrementum pteropi, to the P388 type granulocytic leukemias of cell culture
Toxic action effect substantially, can substantially reduce PGE (PGE) content of inflammatory tissue, but to serum glucocorticoids level without
Significantly affect, show that its antiinflammatory action may be relevant with release with the synthesis for suppressing PGE.
Alum, nitre：According to the classic prescriptions that alum and nitre decocting are boiled, such as alum soup, rheum officinale nitre soup.
Vomiting nut：Alias vomiting nut, active ingredient is Strychnos alkaloid.
The present invention also provides the Chinese medicine composition with antitumor action and is made pharmaceutically acceptable preparation.Said composition
Shared percentage by weight can be 0.1~99.9% in the formulation, and remaining is pharmaceutically acceptable carrier, according to preparation routine side
It is prepared by method.
Composition of the invention, its pharmaceutical dosage forms can be any oral formulations or injection.Wherein oral formulations
Including but not limited to：Conventional tablet, sugar coated tablet, film coated tablet, enteric coated tablet；Capsule, hard capsule, soft capsule
It is agent, oral liquid, buccal tablet, oral disintegrating tablet, granule, electuary, pill, powder, paste, sublimed preparation, supensoid agent, pulvis, pill, micro-
Pill, suppository, creme, spray etc.；Injection is included but is not limited to：Intravenous injection, powder-injection, subcutaneous injection agent etc..This
Invent preferred capsule.Oral 3-5 tablets/time of consumption, 3 times a day.
Composition of the invention, suitable pharmaceutically acceptable carrier, institute are optionally added when medicament is prepared into
Pharmaceutically acceptable carrier is stated to be selected from：Mannitol, sorbierite, sodium pyrosulfite, sodium hydrogensulfite, sodium thiosulfate, hydrochloric acid half
Cystine, TGA, methionine, injection Vitamin B_6 DTA disodiums, Ethylenediaminetetraacetic Acid Calcium Salt, the carbonate of monovalence alkali metal, acetate, phosphorus
Hydrochlorate or its aqueous solution, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose,
Glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its derivative
Thing, alginates, gelatin, polyvinylpyrrolidone, glycerine, Tween 80, agar, calcium carbonate, calcium bicarbonate, polyethylene glycol, ring paste
Essence, beta-schardinger dextrin, phospholipid material, kaolin, talcum powder, calcium stearate, magnesium stearate etc..
In order to preferably prove beneficial effects of the present invention, proved by following test examples.
The antitumor action of the medicine of the present invention of test example 1
First, material
(1) medicine
" commercially available PING XIAO JIAO NANG " is used as former engineer testing medication (Xi'an Zhengda Pharmaceutical Co., Ltd.'s offer)；" medicine of the present invention
Thing PING XIAO JIAO NANG " (being prepared according to the method for embodiment 1) is as experiment medication (in Xi'an Communications University's researches on natural drugs and engineering
The heart is provided).The suspension solution of required concentration is configured to distilled water.
(2) knurl strain
S180Sarcoma tumor-bearing mice (offer of Traditional Chinese Medicine Research Institute, Shanxi Province experimental animal center).
(3) animal
(Xi'an Communications University's Experimental Animal Center is provided ICR strains small white mouse, animal quality quality certification number：The dynamic card of Shan doctor
Word the 08-004th), male and female dual-purpose, body weight 19-22g, pellet is fed.18-22 DEG C of room temperature, relative humidity 60-70%.
2nd, method and result
In extraction intraperitoneal inoculation S under aseptic condition180The sarcoma cell mouse ascites of 1 week, sterile physiological salt is used by 1: 4 times
Water dilutes, and then injects 0.2ml under every mouse left fore internal organs nest under aseptic condition, the weight of animals is claimed after 24h and is grouped, and starts
Gastric infusion, once a day, continuous 10 days, 24h claimed the weight of animals after administration in the tenth day, puts to death mouse, peels off tumor mass and weighs,
Press column count tumour inhibiting rate.The results are shown in Table 1.
Tumour inhibiting rate (%)=(the average average knurl weight of knurl weight-control group of administration group) × average knurl weight of 100%/lotus knurl control group
Tumor-inhibiting action (X ± SD) of the PING XIAO JIAO NANG of table 1 to lotus S180 sarcoma mouse
* P ＜ 0.05
Result shows, each administration group mouse knurl weight is different degrees of less than lotus knurl control group, compared with negative control group,
" commercially available PING XIAO JIAO NANG " heavy dose group and the heavy dose of group of test technology have significant difference；In contrast, medicine of the present invention is each
Dosage group there are no significant difference.On equal tumour inhibiting rate, the dosage of new technology is substantially reduced compared to the dosage of former technique, i.e., newly
Technique population dose is 3-5 tablets/time, and former technique is 4-8 tablets/time.On Isodose, new technology effect is substantially better than former technique
Tumour inhibiting rate.
[Al in the rat blood serum of test example 23+]、[K+] determine
Experiment medication is with test example 1
Purpose：By potassium ion and aluminium in serum after experiment investigation Oral Administration in Rats commercially available " PING XIAO JIAO NANG " and medicine of the present invention
Ion concentration, compares before and after process reform that potassium ion, aluminium ion have indifference in serum, so as to illustrate process reform feasibility.
If variant, illustrate that process reform is larger on potassium ion, aluminium ion influence in serum, then process reform part is infeasible.
(1) [Al in rat blood serum3+] determine
1) experiment material
SD rats, fasting 24h before experiment, respectively by 0.63g/kg (commercially available " PING XIAO JIAO NANG "), 0.45g/kg (medicines of the present invention
Thing) gastric infusion, taken a blood sample each 2ml at following time point 0,1,2,4,6h respectively, be transferred to immediately in centrifuge tube 3000rpm from
Heart 10min, it is stand-by in absorption upper plasma clear liquid to clean centrifuge tube.
2) preparation of blank solution
0.40mL 0.01mol/L hydrochloric acid solutions are drawn in 2mL volumetric flasks, add chromium day cyanines S solution 0.16mL, six times
Tetramine cushioning liquid 0.40mL, plus distilled water is mended to scale, is shaken up.
3) drafting of standard curve
The Al of 2.0 μ g/mL is drawn respectively3+The μ L of standard liquid 80,160,240,320,400 are added in 2mL volumetric flasks
0.01mol/L hydrochloric acid solutions are separately added into chromium day cyanines S solution 0.16mL, hexamethylenetetramine cushioning liquid to 0.40mL
0.40mL, plus distilled water is mended to scale, is shaken up.Colorimetric at 1cm cuvette 550nm wavelength, determines trap.With aluminum standard solution
Concentration be ordinate, draw standard curve.
4) sample determination
Precision is drawn in each 100 μ L to 2mL volumetric flasks of plasma sample, adds 0.01mol/L hydrochloric acid solutions to 0.3mL.Plus
Enter chromium day cyanines S solution 0.16mL, hexamethylenetetramine cushioning liquid 0.40mL, plus distilled water is mended to scale, is shaken up.Use 1cm ratios
Color ware colorimetric at 550nm wavelength, determines trap.
5) result is shown in Fig. 1
Commercially available " PING XIAO JIAO NANG " content, 4h [Al after medication3+] peaking 1.91x10-4mol/L；Oral " medicine of the present invention
4h [Al after thing " content3+] peaking 1.99x10-4mol/L.
(2) [K in rat blood serum+] determine
1) preparation of solution
1. the preparation of potassium standard liquid：The accurate pure potassium chloride 0.1907g of analysis weighed by drying, moves into after water dissolves
In 1000mL volumetric flasks, constant volume to scale is diluted with water, shaken up, as 100ppm potassium.Be diluted to 10 with this liquid, 20,30,40,
A series of potassium standard liquids such as 50 and 60ppm.
2. the preparation of blank solution：10% sodium nitrate 5mL is drawn in 25mL volumetric flasks ,-EDTA the mixing of drop formaldehyde is sheltered plus 5
Agent, 10 drop glycerine and 1 drop phenolphthalein indicator, shake up mixing.1% sodium carbonate is added dropwise and is adjusted to blush, then 0.1N hydrochloric acid tune is added dropwise
It is extremely colourless.The sodium tetraphenylborate precipitation agent 1mL of pH=8 is drawn with the syringe with syringe needle, fast powerful ground injection prepare liquid
In 25mL volumetric flasks, 5 to 10min is placed, add water and be settled to scale, shaken up, as blank solution.
3. 3% sodium tetraphenylborate solution：By 19.45mL 0.2mol/L disodium hydrogen phosphates and 0.55mL 0.1mol/L citric acids
Mixing, as pH=8 cushioning liquid.3.0g tetraphenylboron sodiums are weighed again to be dissolved in cushioning liquid, 100mL is diluted with water to, and are heated
To 70 DEG C of holding 1min, filtering stands overnight, and stores in standby in brown bottle.Cord blood in the dark.
4. formaldehyde-EDTA mixed masking agents：3g EDETATE DISODIUMs are weighed to be dissolved in 90mL water, plus 37% formaldehyde 10mL, stir
Mix well mixed, it is standby.
5. 0.5% phenolphthalein indicator：0.5g phenolphthalein indicators are dissolved in the ethanol of 100mL 95%.
6. 1% sodium carbonate liquor：Constant volume is to 100mL after 1g sodium carbonate water dissolves.
7. 0.1N hydrochloric acid solutions：Take 0.83mL concentrated hydrochloric acids and be diluted to 100mL.
8. 10% sodium nitrate solution：The pure sodium nitrate 100g of analysis is weighed in beaker, is moved into after the 300mL that adds water dissolvings
In 1000mL volumetric flasks, add water and be settled to scale, shake up standby.
2) drafting of standard curve
Potassium standard liquid 5mL is drawn respectively to be put into 25mL volumetric flasks, then drop formaldehyde-EDTA drops close screening agent, 10 drops Jia 5
Glycerine and 1 drop phenolphthalein indicator, shake up mixing.1% sodium carbonate is added dropwise and is adjusted to blush, be adjusted to 0.1N hydrochloric acid colourless.With band
The syringe of syringe needle draws the sodium tetraphenylborate precipitation agent 1mL of pH=8, in the volumetric flask of fast powerful ground injection prepare liquid, makes four
Benzene boron potassium is in superfine particle.5 to 10min is placed, is added water and is settled to scale, shaken up, 1cm cuvettes are used at 440nm wavelength,
Determine light transmittance.With light transmittance as ordinate, potassium concentration is that abscissa draws standard curve.
3) sample determination
Precision draws the μ L of plasma sample 400 in 5mL volumetric flasks, plus 10% sodium nitrate is to 1mL, then Jia 1 and drip formaldehyde-EDTA
Mixed masking agent, 2 drop glycerine and 1 drop phenolphthalein indicator, shake up mixing.1% sodium carbonate is added dropwise and is adjusted to blush, then 0.1N is added dropwise
Hydrochloric acid is adjusted to colourless.The sodium tetraphenylborate precipitation agent 1mL of pH=8 is drawn with the syringe with syringe needle, the injection of fast powerful ground is to be measured
In the volumetric flask of liquid, potassium tetraphenylborate is set to be in superfine particle.5 to 10min is placed, is added water and is settled to scale, shaken up, determine printing opacity
Degree.
4) result is shown in Fig. 2
After commercially available " PING XIAO JIAO NANG " content, 4h [K after medication+] peaking 4.04x10-3mol/L；Oral " medicine of the present invention
After thing " content, 4h [K after medication+] peaking 3.78x10-3mol/L。
(3) conclusion
Experiment finds, after oral " commercially available PING XIAO JIAO NANG " and content obtained in medicine of the present invention, [K in rat blood serum+]、
[Al3+] there was no significant difference.Before and after process modification, serum [K+]、[Al3+] without significant change.Thus point out：Process reform part
To serum [K+]、[Al3+] do not make significant difference, process reform is truly feasible.
Brief description of the drawings
Aluminium ion concentration change curve in Fig. 1 rat blood serums
Potassium concentration change curve in Fig. 2 rat blood serums
Specific embodiment
Present disclosure is explained by following specific embodiment, is not the further limit to the scope of the present invention
It is fixed.
Embodiment 1
Take weight medicinal material：Root tuber of aromatic turmeric 54g, hairyvein agrimony 54g, excrementum pteropi 45g, alum 54g, nitre 54g, dried lacquer 18g processed, bran are fried
Fructus Aurantii 90g, prepared nux vomica 36g；The taste medicinal material root tuber of aromatic turmeric of the above 8, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed are ground into most meal, according to stream preserved material and leaching
The lower percolation of paste, uses 70% ethanol percolation, collects percolate, ethanol is recovered under reduced pressure and obtains extractum A；Take hairyvein agrimony, alum, nitre
Stone, excrementum pteropi are added water to cook 2 times with the above-mentioned dregs of a decoction, and 1 hour every time, decoction liquor filtering merged, and filtering, filtrate concentrate drying obtains powder
Last B；Above-mentioned extractum A, powder B and prepared nux vomica are mixed, and pelletized, are dried, encapsulated.
Embodiment 2
Take weight medicinal material：Root tuber of aromatic turmeric 30g, hairyvein agrimony 30g, excrementum pteropi 25g, alum 30g, nitre 30g, dried lacquer 10g processed, bran are fried
Fructus Aurantii 50g, prepared nux vomica 20g；The taste medicinal material root tuber of aromatic turmeric of the above 8, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed are ground into most meal, according to stream preserved material and leaching
The lower percolation of paste, is extracted with 60% alcohol reflux, collects extract solution, ethanol is recovered under reduced pressure and obtains extractum A；Take hairyvein agrimony, white
Alum, nitre, excrementum pteropi are added water to cook 1 time with the above-mentioned dregs of a decoction, and 3 hours every time, decoction liquor filtering merged, filtering, and filtrate concentration is dry
It is dry to obtain powder B；Above-mentioned extractum A, powder B and prepared nux vomica are mixed.
Embodiment 3
Take weight medicinal material：Root tuber of aromatic turmeric 10g, hairyvein agrimony 10g, excrementum pteropi 10g, alum 10g, nitre 10g, dried lacquer 5g processed, bran are fried
Fructus Aurantii 30g, prepared nux vomica 5g；The taste medicinal material root tuber of aromatic turmeric of the above 8, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed are crushed and extracted with 80% EtOH Sonicate, are collected
Extract solution, is recovered under reduced pressure ethanol and obtains extractum A；Take hairyvein agrimony, alum, nitre, excrementum pteropi and the above-mentioned dregs of a decoction and add water to cook 3 times, often
Secondary 2 hours, decoction liquor filtering merged, and filtering, filtrate concentrate drying obtains powder B；Above-mentioned extractum A, powder B and prepared nux vomica are mixed
.
Embodiment 4
Take weight medicinal material：Root tuber of aromatic turmeric 50g, hairyvein agrimony 50g, excrementum pteropi 50g, alum 50g, nitre 50g, dried lacquer 60g processed, bran are fried
Fructus Aurantii 70g, prepared nux vomica 40g；The taste medicinal material root tuber of aromatic turmeric of the above 8, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed are ground into most meal, according to stream preserved material and leaching
The lower percolation of paste, is extracted with 80% ethanol percolation, collects percolate, ethanol is recovered under reduced pressure and obtains extractum A；Take hairyvein agrimony, white
Alum, nitre, excrementum pteropi are added water to cook 1 time with the above-mentioned dregs of a decoction, and 3 hours every time, decoction liquor filtering merged, filtering, and filtrate concentration is dry
It is dry to obtain powder B；Above-mentioned extractum A, powder B and prepared nux vomica are mixed.
Embodiment 5
Take weight medicinal material：Root tuber of aromatic turmeric 40g, hairyvein agrimony 40g, excrementum pteropi 40g, alum 40g, nitre 40g, dried lacquer 20g processed, bran are fried
Fructus Aurantii 60g, prepared nux vomica 30g；The taste medicinal material root tuber of aromatic turmeric of the above 8, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed are ground into most meal, according to stream preserved material and leaching
The lower percolation of paste, is extracted with 70% ethanol percolation, collects percolate, ethanol is recovered under reduced pressure and obtains extractum A；Take hairyvein agrimony, white
Alum, nitre, excrementum pteropi are added water to cook 2 times with the above-mentioned dregs of a decoction, and 1 hour every time, decoction liquor filtering merged, filtering, and filtrate concentration is dry
It is dry to obtain powder B；Above-mentioned extractum A, powder B and prepared nux vomica are mixed.
Embodiment 6, granule
Any extractum As of Example 1-5, powder B and prepared nux vomica mix 100g, are separately added into 1.5 times of dextrin of amount,
0.5% sucrose, 1.5% microcrystalline cellulose is dissolved with ethanol in proper amount and is made softwood, is pelletized, 60 DEG C of forced air dryings, granulation, whole grain,
Obtain final product granule.
Embodiment 7, dripping pill
Any extractum As of Example 1-5, powder B and prepared nux vomica mix 100g, add the polyethylene glycol of 1000g, mixing
Uniformly, melt, upper pill dripping machine is made dripping pill.
Embodiment 8, oral disnitegration tablet
Any extractum As of Example 1-5, powder B and prepared nux vomica mix 100g, add 5% PVPP, 0.1%
Magnesium stearate, 50% microcrystalline cellulose is made softwood with ethanol in proper amount solution, pelletizes, 60 DEG C of forced air dryings, and granulation is whole
Grain, it is tabletted, obtain final product oral disnitegration tablet.
Embodiment 9, powder-injection
Any extractum As of Example 1-5, powder B and prepared nux vomica mix 0.5g, glucose 4.5g, sodium thiosulfate
0.9g and distilled water 1ml, after said components are well mixed, freeze-drying dispenses 500, obtains final product powder-injection.
Embodiment 10, tablet
Any extractum As of Example 1-5, powder B and prepared nux vomica mix 100g, with starch, sodium carboxymethylcellulose, cunning
Stone flour is well mixed, and granulation, compressing tablet obtains final product tablet.
Embodiment 11, oral liquid
Any extractum As of Example 1-5, powder B and prepared nux vomica mix 2g, with syrup 4g, are dissolved in the pure water of 100ml
In, homogeneous, filtering, by high-temperature short-time sterilization (135 DEG C, 4s).Sterile filling, packing.Oral liquid is obtained.
Group component in the range of above-described embodiment and specification can need to expand or shrink ratio simultaneously according to production.
Claims (8)
1. a kind of Traditional Chinese medicine composition with anti-tumor effect, it is characterised in that obtained by following step preparation method：
(1) following medicinal materials are taken by weight：Root tuber of aromatic turmeric 1-5 parts, hairyvein agrimony 1-5 parts, excrementum pteropi 1-5 parts, alum 1-5 parts, nitre 1-5
Part, dried lacquer 0.5-3 parts processed, stir-baked FRUCTUS AURANTII in bran 3-7 parts, prepared nux vomica 0.5-4 parts；
(2) 8 taste medicinal material more than, takes described root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed and crushes, and uses 60-80% ethanol percolations, and collection is oozed
Filter liquid, ethanol is recovered under reduced pressure and obtains extractum A, the dregs of a decoction are standby；
(3) take hairyvein agrimony, alum, nitre, excrementum pteropi to be added water to cook 1-3 times with the above-mentioned dregs of a decoction, each 1-3 hours, decoction liquor mistake
Filter merges, and filtering, filtrate concentrate drying obtains powder B；
(4) above-mentioned extractum A, powder B and prepared nux vomica are mixed.
2. Chinese medicine composition as claimed in claim 1, it is characterised in that take weight portion medicinal material root tuber of aromatic turmeric 2-4 described in step (1)
Part, hairyvein agrimony 2-4 parts, excrementum pteropi 2-4 parts, alum 2-4 parts, nitre 2-4 parts, dried lacquer 0.5-2 parts processed, stir-baked FRUCTUS AURANTII in bran 4-6 parts, horse
1-3 parts of money powder.
3. Chinese medicine composition as claimed in claim 1, it is characterised in that take weight portion medicinal material root tuber of aromatic turmeric 3 described in step (1)
Part, 3 parts of hairyvein agrimony, 2.5 parts of excrementum pteropi, 3 parts of alum, 3 parts of nitre, 1 part of dried lacquer processed, 5 parts of stir-baked FRUCTUS AURANTII in bran, 2 parts of prepared nux vomica.
4. Chinese medicine composition as claimed in claim 1, it is characterised in that：Root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, system described in step (2)
Dried lacquer is crushed, and uses 70% ethanol percolation, collects percolate, ethanol is recovered under reduced pressure and obtains extractum A.
5. Chinese medicine composition as claimed in claim 1, it is characterised in that：Hairyvein agrimony, alum, nitre are taken described in step (3)
Stone, excrementum pteropi are added water to cook 2 times with the above-mentioned dregs of a decoction, and 2 hours every time, decoction liquor filtering merged, and filtering, filtrate concentrate drying obtains powder
Last B.
6. a kind of pharmaceutical preparation, it is characterised in that Chinese medicine composition as described in claim 1-5 any one as activity into
Divide and be prepared from.
7. a kind of Traditional Chinese medicine composition with anti-tumor effect preparation method, it is characterised in that comprise the following steps：
(1) following medicinal materials are taken by weight：Root tuber of aromatic turmeric 1-5 parts, hairyvein agrimony 1-5 parts, excrementum pteropi 1-5 parts, alum 1-5 parts, nitre 1-5
Part, dried lacquer 0.5-3 parts processed, stir-baked FRUCTUS AURANTII in bran 3-7 parts, prepared nux vomica 0.5-4 parts；
(2) 8 taste medicinal material more than, takes described root tuber of aromatic turmeric, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed and crushes, and uses 60-80% ethanol percolations, and collection is oozed
Filter liquid, ethanol is recovered under reduced pressure and obtains extractum A, the dregs of a decoction are standby；
(3) take hairyvein agrimony, alum, nitre, excrementum pteropi to be added water to cook 1-3 times with the above-mentioned dregs of a decoction, each 1-3 hours, decoction liquor mistake
Filter merges, and filtering, filtrate concentrate drying obtains powder B；
(4) above-mentioned extractum A, powder B and prepared nux vomica are mixed.
8. preparation method as claimed in claim 7, it is characterised in that comprise the following steps：
(1) 3 parts of weight portion medicinal material root tuber of aromatic turmeric, 3 parts of hairyvein agrimony, 2.5 parts of excrementum pteropi, 3 parts of alum, 3 parts of nitre, 1 part of dried lacquer processed, bran are taken
Fry 5 parts of Fructus Aurantii, 2 parts of prepared nux vomica；
(2) 8 taste medicinal material root tubers of aromatic turmeric more than, stir-baked FRUCTUS AURANTII in bran, dried lacquer processed are crushed, and use 70% ethanol percolation, collect percolate, are recovered under reduced pressure
Ethanol obtains extractum A, and the dregs of a decoction are standby；
(3) take hairyvein agrimony, alum, nitre, excrementum pteropi to be added water to cook 2 times with the above-mentioned dregs of a decoction, 2 hours every time, decoction liquor filtering was closed
And, filtering, filtrate concentrate drying obtains powder B；
(4) above-mentioned extractum A, powder B and prepared nux vomica are mixed.
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| CN104958476A (en) * | 2015-07-03 | 2015-10-07 | 刘明璇 | External medicament for treating tumor and preparation method thereof |
| CN106389872A (en) * | 2016-09-26 | 2017-02-15 | 魏庆 | Traditional Chinese medicine composition for preventing and treating tumors and preparation method of traditional Chinese medicine composition for preventing and treating tumors |
| CN114642714A (en) * | 2020-12-18 | 2022-06-21 | 西安正大制药有限公司 | Anti-tumor traditional Chinese medicine composition extract, preparation method and application thereof in inhibiting breast cancer lung metastasis |
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