CN103548815A - Programmed cell freezing box with direct-in liquid nitrogen - Google Patents
Programmed cell freezing box with direct-in liquid nitrogen Download PDFInfo
- Publication number
- CN103548815A CN103548815A CN201310576212.XA CN201310576212A CN103548815A CN 103548815 A CN103548815 A CN 103548815A CN 201310576212 A CN201310576212 A CN 201310576212A CN 103548815 A CN103548815 A CN 103548815A
- Authority
- CN
- China
- Prior art keywords
- box
- box body
- cell
- liquid nitrogen
- programmed cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 239000007788 liquid Substances 0.000 title claims abstract description 26
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 22
- 238000007710 freezing Methods 0.000 title abstract description 30
- 230000008014 freezing Effects 0.000 title abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 13
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000741 silica gel Substances 0.000 claims abstract description 8
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 229920001903 high density polyethylene Polymers 0.000 claims abstract description 6
- 239000004700 high-density polyethylene Substances 0.000 claims abstract description 6
- 239000004033 plastic Substances 0.000 claims abstract description 6
- 229920003023 plastic Polymers 0.000 claims abstract description 6
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 238000005476 soldering Methods 0.000 claims abstract description 4
- 239000010935 stainless steel Substances 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims description 22
- 230000008595 infiltration Effects 0.000 claims description 3
- 238000001764 infiltration Methods 0.000 claims description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 abstract description 14
- 238000013461 design Methods 0.000 abstract description 2
- 239000000945 filler Substances 0.000 abstract description 2
- 239000010963 304 stainless steel Substances 0.000 abstract 2
- 229910000589 SAE 304 stainless steel Inorganic materials 0.000 abstract 2
- 239000006260 foam Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 65
- 239000000243 solution Substances 0.000 description 18
- 230000006378 damage Effects 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 238000000034 method Methods 0.000 description 12
- 239000013078 crystal Substances 0.000 description 10
- 238000004321 preservation Methods 0.000 description 9
- 230000009467 reduction Effects 0.000 description 7
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 238000005138 cryopreservation Methods 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 229920002274 Nalgene Polymers 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000003463 organelle Anatomy 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 108010019160 Pancreatin Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 101150032953 ins1 gene Proteins 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229940055695 pancreatin Drugs 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102100023374 Forkhead box protein M1 Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 101000907578 Homo sapiens Forkhead box protein M1 Proteins 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 101100221606 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) COS7 gene Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Images
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310576212.XA CN103548815B (en) | 2013-11-18 | 2013-11-18 | Programmed cell freezing box with direct-in liquid nitrogen |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310576212.XA CN103548815B (en) | 2013-11-18 | 2013-11-18 | Programmed cell freezing box with direct-in liquid nitrogen |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103548815A true CN103548815A (en) | 2014-02-05 |
CN103548815B CN103548815B (en) | 2015-06-10 |
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Family Applications (1)
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CN201310576212.XA Active CN103548815B (en) | 2013-11-18 | 2013-11-18 | Programmed cell freezing box with direct-in liquid nitrogen |
Country Status (1)
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CN (1) | CN103548815B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107258766A (en) * | 2017-06-18 | 2017-10-20 | 广东博溪生物科技有限公司 | A kind of cell freezing method and cells frozen storing liquid |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4377077A (en) * | 1981-07-15 | 1983-03-22 | Biotech Research Laboratories, Inc. | Device and method for controlled freezing of cell cultures |
US4455842A (en) * | 1981-07-15 | 1984-06-26 | Biotech Research Laboratories, Inc. | Device and method for controlled freezing of cell cultures |
WO2005066559A2 (en) * | 2004-01-08 | 2005-07-21 | Bernhard Sixt | Transport container for keeping frozen material chilled |
CN203040527U (en) * | 2013-02-25 | 2013-07-10 | 中国水产科学研究院黑龙江水产研究所 | Cell freezing and storing box |
CN103202286A (en) * | 2013-04-11 | 2013-07-17 | 天津开发区合普工贸有限公司 | Electronic program-controlled const-speed cooling and freezing device |
-
2013
- 2013-11-18 CN CN201310576212.XA patent/CN103548815B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4377077A (en) * | 1981-07-15 | 1983-03-22 | Biotech Research Laboratories, Inc. | Device and method for controlled freezing of cell cultures |
US4455842A (en) * | 1981-07-15 | 1984-06-26 | Biotech Research Laboratories, Inc. | Device and method for controlled freezing of cell cultures |
WO2005066559A2 (en) * | 2004-01-08 | 2005-07-21 | Bernhard Sixt | Transport container for keeping frozen material chilled |
CN203040527U (en) * | 2013-02-25 | 2013-07-10 | 中国水产科学研究院黑龙江水产研究所 | Cell freezing and storing box |
CN103202286A (en) * | 2013-04-11 | 2013-07-17 | 天津开发区合普工贸有限公司 | Electronic program-controlled const-speed cooling and freezing device |
Non-Patent Citations (1)
Title |
---|
程方杰: "《材料成型与控制实验教程 焊接分册》", 31 August 2011 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107258766A (en) * | 2017-06-18 | 2017-10-20 | 广东博溪生物科技有限公司 | A kind of cell freezing method and cells frozen storing liquid |
Also Published As
Publication number | Publication date |
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CN103548815B (en) | 2015-06-10 |
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C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Wang Yi Inventor after: Hu Lidan Inventor after: An Man Inventor before: Wang Yi Inventor before: Hu Lidan |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20171227 Address after: No. 9, innovation building, No. 9, Hsiang Tong Road, Hi-tech Zone, Hunan, Hunan Patentee after: XIANGTAN ZHILIAN TECHNOLOGY MATASTASIS PROMOTE Co.,Ltd. Address before: 100190 Beijing, Zhongguancun, north of a No. 11, No. Patentee before: Wang Yi |
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TR01 | Transfer of patent right |
Effective date of registration: 20180319 Address after: Heng Lu garden East Lake New Technology Development Zone 430000 Hubei city of Wuhan province No. 3 Lingyun Industrial Park 3 Building No. 304 electro-optic Patentee after: NKY YUEBO (WUHAN) MEDICAL TECHNOLOGY CO.,LTD. Address before: No. 9, innovation building, No. 9, Hsiang Tong Road, Hi-tech Zone, Hunan, Hunan Patentee before: XIANGTAN ZHILIAN TECHNOLOGY MATASTASIS PROMOTE Co.,Ltd. |
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Effective date of registration: 20200909 Address after: Room 1801, 18 / F, 159 qiaozhong Road, Liwan District, Guangzhou City, Guangdong Province (office only) Patentee after: Best (Guangdong) precision medical technology Co.,Ltd. Address before: Heng Lu garden East Lake New Technology Development Zone 430000 Hubei city of Wuhan province No. 3 Lingyun Industrial Park 3 Building No. 304 electro-optic Patentee before: NKY YUEBO (WUHAN) MEDICAL TECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20220921 Address after: Room 102, Building 5, Block II-6, Guannan Science and Technology Industrial Park, Donghu New Technology Development Zone, Wuhan City, Hubei Province, 430205 Patentee after: WUHAN FEISITE BIOTECHNOLOGY CO.,LTD. Address before: 510145 room 1801, 18th floor, 159 qiaozhong Middle Road, Liwan District, Guangzhou City, Guangdong Province (office only) Patentee before: Best (Guangdong) precision medical technology Co.,Ltd. |
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Effective date of registration: 20221102 Address after: 610, Floor 6, Block A, No. 2, Lize Middle Second Road, Chaoyang District, Beijing 100102 Patentee after: Zhongguancun Technology Leasing Co.,Ltd. Address before: Room 102, Building 5, Block II-6, Guannan Science and Technology Industrial Park, Donghu New Technology Development Zone, Wuhan City, Hubei Province, 430205 Patentee before: WUHAN FEISITE BIOTECHNOLOGY CO.,LTD. |