CN104222068B - Low-temperature preserved solid culture medium of skin model and preservation method of low-temperature preserved solid culture medium - Google Patents
Low-temperature preserved solid culture medium of skin model and preservation method of low-temperature preserved solid culture medium Download PDFInfo
- Publication number
- CN104222068B CN104222068B CN201410418456.XA CN201410418456A CN104222068B CN 104222068 B CN104222068 B CN 104222068B CN 201410418456 A CN201410418456 A CN 201410418456A CN 104222068 B CN104222068 B CN 104222068B
- Authority
- CN
- China
- Prior art keywords
- skin model
- culture medium
- liquid
- cryopreservation
- low
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000007787 solid Substances 0.000 title claims abstract description 40
- 239000001963 growth medium Substances 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000004321 preservation Methods 0.000 title abstract description 10
- 238000005138 cryopreservation Methods 0.000 claims description 47
- 239000007788 liquid Substances 0.000 claims description 43
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 claims description 39
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims description 36
- 230000008520 organization Effects 0.000 claims description 36
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 30
- 239000002609 medium Substances 0.000 claims description 28
- 229920000936 Agarose Polymers 0.000 claims description 27
- 210000004027 cell Anatomy 0.000 claims description 21
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 19
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 19
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 19
- 235000004279 alanine Nutrition 0.000 claims description 19
- 229940087168 alpha tocopherol Drugs 0.000 claims description 19
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 19
- 229960000984 tocofersolan Drugs 0.000 claims description 19
- 239000002076 α-tocopherol Substances 0.000 claims description 19
- 235000004835 α-tocopherol Nutrition 0.000 claims description 19
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims description 18
- UBQYURCVBFRUQT-UHFFFAOYSA-N N-benzoyl-Ferrioxamine B Chemical compound CC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCNC(=O)CCC(=O)N(O)CCCCCN UBQYURCVBFRUQT-UHFFFAOYSA-N 0.000 claims description 18
- 229960000958 deferoxamine Drugs 0.000 claims description 18
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 17
- 229960005305 adenosine Drugs 0.000 claims description 17
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 15
- 239000004471 Glycine Substances 0.000 claims description 15
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 15
- 229960005070 ascorbic acid Drugs 0.000 claims description 15
- 239000005720 sucrose Substances 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000012930 cell culture fluid Substances 0.000 claims description 12
- 229930091371 Fructose Natural products 0.000 claims description 9
- 229960002737 fructose Drugs 0.000 claims description 9
- 239000005715 Fructose Substances 0.000 claims description 8
- 229960003767 alanine Drugs 0.000 claims description 8
- RNBGYGVWRKECFJ-ZXXMMSQZSA-N alpha-D-fructofuranose 1,6-bisphosphate Chemical compound O[C@H]1[C@H](O)[C@](O)(COP(O)(O)=O)O[C@@H]1COP(O)(O)=O RNBGYGVWRKECFJ-ZXXMMSQZSA-N 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 8
- 239000001177 diphosphate Substances 0.000 claims description 8
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims description 8
- 235000011180 diphosphates Nutrition 0.000 claims description 8
- 229940025237 fructose 1,6-diphosphate Drugs 0.000 claims description 8
- 210000001339 epidermal cell Anatomy 0.000 claims description 7
- 229960002449 glycine Drugs 0.000 claims description 7
- 239000004615 ingredient Substances 0.000 claims description 7
- 238000002844 melting Methods 0.000 claims description 7
- 230000008018 melting Effects 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 229960004793 sucrose Drugs 0.000 claims description 3
- 229940074410 trehalose Drugs 0.000 claims description 3
- 239000002577 cryoprotective agent Substances 0.000 claims description 2
- 210000003491 skin Anatomy 0.000 abstract description 68
- 210000001519 tissue Anatomy 0.000 abstract description 20
- 230000006378 damage Effects 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 12
- 238000002156 mixing Methods 0.000 abstract description 6
- 210000002615 epidermis Anatomy 0.000 abstract description 3
- 238000004113 cell culture Methods 0.000 abstract description 2
- 239000011814 protection agent Substances 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 239000011543 agarose gel Substances 0.000 abstract 1
- 238000011084 recovery Methods 0.000 description 12
- 230000032258 transport Effects 0.000 description 12
- 208000027418 Wounds and injury Diseases 0.000 description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 9
- 229910052760 oxygen Inorganic materials 0.000 description 9
- 239000001301 oxygen Substances 0.000 description 9
- 239000003223 protective agent Substances 0.000 description 9
- 230000002950 deficient Effects 0.000 description 8
- 208000014674 injury Diseases 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 210000000170 cell membrane Anatomy 0.000 description 7
- 150000003254 radicals Chemical class 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 230000003321 amplification Effects 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 5
- 239000012531 culture fluid Substances 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 240000000111 Saccharum officinarum Species 0.000 description 4
- 235000007201 Saccharum officinarum Nutrition 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000006907 apoptotic process Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 210000004292 cytoskeleton Anatomy 0.000 description 2
- 230000000254 damaging effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000034659 glycolysis Effects 0.000 description 2
- 210000003701 histiocyte Anatomy 0.000 description 2
- 210000002752 melanocyte Anatomy 0.000 description 2
- 230000002438 mitochondrial effect Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 239000006163 transport media Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical group [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229950006790 adenosine phosphate Drugs 0.000 description 1
- 230000004103 aerobic respiration Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000006567 cellular energy metabolism Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000003436 cytoskeletal effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229960001031 glucose Drugs 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000037427 ion transport Effects 0.000 description 1
- ZKLLSNQJRLJIGT-UYFOZJQFSA-N keto-D-fructose 1-phosphate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)COP(O)(O)=O ZKLLSNQJRLJIGT-UYFOZJQFSA-N 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000002676 xenobiotic agent Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410418456.XA CN104222068B (en) | 2014-08-22 | 2014-08-22 | Low-temperature preserved solid culture medium of skin model and preservation method of low-temperature preserved solid culture medium |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410418456.XA CN104222068B (en) | 2014-08-22 | 2014-08-22 | Low-temperature preserved solid culture medium of skin model and preservation method of low-temperature preserved solid culture medium |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104222068A CN104222068A (en) | 2014-12-24 |
CN104222068B true CN104222068B (en) | 2017-01-18 |
Family
ID=52211684
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410418456.XA Active CN104222068B (en) | 2014-08-22 | 2014-08-22 | Low-temperature preserved solid culture medium of skin model and preservation method of low-temperature preserved solid culture medium |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104222068B (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105087464A (en) * | 2015-08-17 | 2015-11-25 | 陕西博溪生物科技有限公司 | Full-thickness skin model for in-vitro alternative tests and preparation method of full-thickness skin model |
CN105219700B (en) * | 2015-10-23 | 2019-09-20 | 陕西艾尔肤组织工程有限公司 | A kind of gel and its preparation method and application |
CN105230608B (en) * | 2015-10-23 | 2023-05-09 | 陕西艾尔肤组织工程有限公司 | Preservation method of tissue engineering skin and tissue engineering skin |
CN107164306B (en) * | 2017-06-18 | 2020-09-08 | 广东博溪生物科技有限公司 | Solid culture medium for cornea model low-temperature preservation and preparation method and application method thereof |
CN109699631B (en) * | 2018-12-21 | 2021-07-16 | 广州悦清再生医学科技有限公司 | Biological material semi-solid storage medium and application |
CN111100838B (en) * | 2019-12-27 | 2023-05-09 | 广东博溪生物科技有限公司 | Low-temperature preservation and transportation culture medium |
CN111631212B (en) * | 2020-06-28 | 2021-12-24 | 济南磐升生物技术有限公司 | Low-temperature storage method of multifunctional 3D recombinant skin model |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4299819A (en) * | 1979-01-02 | 1981-11-10 | Sloan-Kettering Institute For Cancer Research | Process for treating burn victims |
US5635344A (en) * | 1994-12-07 | 1997-06-03 | Cedra Corp. | Shipping medium for organ-derived cells |
CN101486995B (en) * | 2009-02-24 | 2011-01-05 | 中国检验检疫科学研究院 | Culture medium for tissue engineering skin convey and formulating method thereof |
-
2014
- 2014-08-22 CN CN201410418456.XA patent/CN104222068B/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN104222068A (en) | 2014-12-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104222068B (en) | Low-temperature preserved solid culture medium of skin model and preservation method of low-temperature preserved solid culture medium | |
CN101720753B (en) | Cryopreservation solution of tissue engineering products and application method thereof | |
CN104145943B (en) | A kind of frozen protection liquid of people's umbilical cord China Tong Shi glue tissue and preparation and application thereof | |
CN101037666B (en) | Low temperature preservation solution for animal cells and preserving method | |
CN102648708B (en) | Freezing liquid for embryo or cells and application thereof | |
CN103215208B (en) | Haemophilus parasuis culture medium | |
CN103548814B (en) | Non-programmable-controlled cooling cryopreservation method and protection agent for hematopoietic stem cells at minus 80DEG C | |
CN108094404A (en) | A kind of improved mesenchyme stem cell protection solution and application thereof | |
CN104162314B (en) | The extraction process of a kind of effective ingredients in plant and application thereof | |
CN102428911A (en) | Cryogenic storing protection liquid for human umbilical cord Wharton's jelly tissue block | |
CN102640745B (en) | Ultralow-temperature cryopreservation and recovery method for embryonic materials | |
CN104938478B (en) | A kind of articular cartilage glass freezing protection liquid and cartilage store method | |
CN102726366A (en) | Organ preservation solution and method for preparing same | |
CN101368172A (en) | Freeze drying protective agent for rubricyte and use method in freeze drying process | |
CN113925049B (en) | Cell preservation solution for maintaining cell activity and preparation method and application thereof | |
Moussa et al. | Cell inactivation and membrane damage after long‐term treatments at sub‐zero temperature in the supercooled and frozen states | |
CN111011363A (en) | Mesenchymal stem cell cryopreservation liquid, cryopreservation method, preservation kit and recovery method | |
CN101070534B (en) | Method for freezing preservation nervous stem cells | |
CN105211052B (en) | Frozen stock solution of cultured NKT cells and preparation method thereof | |
CN102771472B (en) | Freezing liquid for preserving embryo, preparation method and application thereof | |
CN111100838B (en) | Low-temperature preservation and transportation culture medium | |
CN103999849B (en) | The antifreeze of the freezing preservation of a kind of mammal testis tissue and freeze-thaw method | |
CN105325402A (en) | Cryopreservation protective agent for vitrification cryopreservation of marrow mesenchymal stem cells | |
CN107164306B (en) | Solid culture medium for cornea model low-temperature preservation and preparation method and application method thereof | |
CN100519742C (en) | Ultra-low temperature preservation solution for animal cells and preserving method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
ASS | Succession or assignment of patent right |
Owner name: SHAANXI BOXI BIOLOGICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SHAANXI BOHONG BIOTECHNOLOGY CO., LTD. Effective date: 20141223 |
|
C41 | Transfer of patent application or patent right or utility model | ||
C53 | Correction of patent for invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhi Xubo Inventor after: Lu Yongbo Inventor after: Li Xiao Inventor before: Zhi Xubo Inventor before: Xu Jiajie Inventor before: Luo Hailang Inventor before: Lu Yongbo Inventor before: Li Xiao |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: ZHI XUBO XU JIAJIE LUO HAILANG LU YONGBO LI XIAO TO: ZHI XUBO LU YONGBO LI XIAO Free format text: CORRECT: ADDRESS; FROM: 710025 XI'AN, SHAANXI PROVINCE TO: 710065 XI'AN, SHAANXI PROVINCE |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20141223 Address after: 710065 Shaanxi City, south of the Second Ring Road, Qinglong District Albert urban vision, No. 1 10C Applicant after: SHAANXI BIOCELL BIOTECHNOLOGY CO., LTD. Address before: 710025, Shaanxi District, Xi'an province Baqiao Textile Industrial Park standard factory building, B1 building, 4 floor Applicant before: Shaanxi Bohong Biotechnology Co., Ltd. |
|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information |
Address after: 523808, 1, building 1, 1, Taoyuan biotechnology cooperation center, Taoyuan hi tech Industrial Development Zone, Songshan hi tech Industrial Development Zone, Guangdong, Dongguan, Taiwan, China 08 Applicant after: Guangdong Bo Xi Biotechnology Co., Ltd. Address before: 710065 Shaanxi City, south of the Second Ring Road, Qinglong District Albert urban vision, No. 1 10C Applicant before: SHAANXI BIOCELL BIOTECHNOLOGY CO., LTD. |
|
COR | Change of bibliographic data | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Low-temperature preserved solid culture medium of skin model and preservation method of low-temperature preserved solid culture medium Effective date of registration: 20200426 Granted publication date: 20170118 Pledgee: Xianyang corporate finance Company limited by guarantee Pledgor: GUANGDONG BIOCELL BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020980001783 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210528 Granted publication date: 20170118 Pledgee: Xianyang corporate finance Company limited by guarantee Pledgor: GUANGDONG BIOCELL BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020980001783 |