CN103548665A - Laboratory Limnoperna lacustris culture device and method for culturing Limnoperna lacustris by using same - Google Patents
Laboratory Limnoperna lacustris culture device and method for culturing Limnoperna lacustris by using same Download PDFInfo
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- CN103548665A CN103548665A CN201310543654.4A CN201310543654A CN103548665A CN 103548665 A CN103548665 A CN 103548665A CN 201310543654 A CN201310543654 A CN 201310543654A CN 103548665 A CN103548665 A CN 103548665A
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- 238000000034 method Methods 0.000 title claims abstract description 15
- 241001091139 Limnoperna Species 0.000 title abstract 7
- 238000012258 culturing Methods 0.000 title abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000001179 sorption measurement Methods 0.000 claims abstract description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000001301 oxygen Substances 0.000 claims abstract description 9
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 9
- 241000237536 Mytilus edulis Species 0.000 claims description 56
- 239000013505 freshwater Substances 0.000 claims description 56
- 235000020638 mussel Nutrition 0.000 claims description 56
- 239000003463 adsorbent Substances 0.000 claims description 17
- 238000005192 partition Methods 0.000 claims description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 12
- 241000195493 Cryptophyta Species 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 229910052742 iron Inorganic materials 0.000 claims description 6
- 239000004567 concrete Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 2
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 2
- 241001330002 Bambuseae Species 0.000 claims description 2
- 229920005479 Lucite® Polymers 0.000 claims description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 2
- 239000011425 bamboo Substances 0.000 claims description 2
- 230000003203 everyday effect Effects 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 3
- 238000012136 culture method Methods 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 238000004378 air conditioning Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 241000237525 Mytilidae Species 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/60—Fishing; Aquaculture; Aquafarming
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- Hydroponics (AREA)
- Water Treatment By Sorption (AREA)
Abstract
The invention relates to a laboratory Limnoperna lacustris culture device and a method for culturing Limnoperna lacustris by using the same, and belongs to the field of Limnoperna lacustris research. The culture device comprises a culture pond, an internal baffle and an adsorption device, wherein the left sidewall of the culture pond is provided with a water inlet, and the right sidewall of the culture pond is provided with a water outlet; the internal baffle consists of a front baffle and a back baffle, and the adsorption device is arranged between the front baffle and the back baffle. In the culture stage, nutrients are added into the culture pond daily, the temperature of water is maintained at 22 plus or minus 2 DEG C, the flow rate of raw water in the culture pond is 0.1 to 0.2m/s, the concentration of dissolved oxygen in water is 2.2 to 3mg/L, the pH of water is controlled between 6.5 and 9, and water on the culture pond is replaced once every 10 to 14 days. The culture device provided by the invention is simple in structure, the culturing is easily realized under laboratory conditions, and an attachment device is favorable for attachment and growth of Limnoperna lacustris; the culture method is simple and feasible, the Limnoperna lacustris can be cultured under the laboratory conditions, and the better technical effects can be obtained.
Description
Technical field
The invention belongs to fresh water mussel research field, be specifically related to a kind of device of laboratory cultures fresh water mussel and utilize said apparatus to carry out the method for fresh water mussel cultivation.
Background technology
Along with coming into operation of long distance water transfer project, in water-carriage system, biotic intrusion and stained problem also highlight gradually." biodeterioration " refers to that a large amount of attaching biologicals intrude in water delivery structure and associated production equipment and adheres to, and density is up to 10000 ~ 100000/m
2, stained to body structure surface generation, corrosion structure, even blocking pipe, affects the normal operation of engineering.The Mytilidae species fresh water mussel of south China area growth has caused biodeterioration serious in aqueduct, these shellfishes ceaselessly adhere to, grow, breed in waterline, etching apparatus, blocking pipe, increase resistance to water-flow, mortality after stain water environment, causes many harmful effects, fresh water mussel adheres in the important feature such as aqueduct wall and seam, gate valve, even can cause security incident.In recent years, about how killing the research of fresh water mussel in aqueduct, getting more and more, therefore needing a kind of method of laboratory cultures fresh water mussel.
Summary of the invention
The object of the present invention is to provide a kind of device for laboratory cultures fresh water mussel and utilize said apparatus to carry out the method for fresh water mussel laboratory cultures.
Laboratory of the present invention fresh water mussel culture apparatus is comprised of culture pond, internal partition and adsorbent equipment; The left side wall of described culture pond is provided with water inlet, and right side wall is provided with delivery port, and the inside of culture pond is provided with internal partition and the adsorbent equipment that is parallel to left and right sidewall; Described internal partition is comprised of front dividing plate and rear bulkhead, the front end of front dividing plate is close to the antetheca of culture pond, the rear wall of culture pond is close in the rear end of rear bulkhead, and front dividing plate and rear bulkhead alternative arrangement form revolution gallery type, between front dividing plate and rear bulkhead, are provided with adsorbent equipment.
Utilize above-mentioned culture apparatus to carry out the cultivation of fresh water mussel, whole incubation need be carried out under lucifuge condition.At cultivation stage, add nutriment to meet the growth demand of fresh water mussel every day in culture apparatus, laboratory ventilation is good, control temperature by air-conditioning summer, places heating rod winter in water body, keeps water temperature 22 ± 2 ℃ of left and right, former water in casing flow control at 0.1 ~ 0.2m/s, oxygen in water concentration is 2.2 ~ 3mg/L left and right, and water body pH need be controlled at 6.5 ~ 9, and the water in culture apparatus is changed once for every 10 ~ 14 days.
The attachment characteristic of fresh water mussel is considered and utilized to culture apparatus of the present invention, simple in structure, under laboratory condition, easily realizes, and attaching device is conducive to adhering to of fresh water mussel and grows.
The cultural method of fresh water mussel of the present invention, simple, can under laboratory condition, realize the cultivation to fresh water mussel, obtain good technique effect.
Accompanying drawing explanation
Fig. 1 is the vertical view of fresh water mussel culture apparatus;
Fig. 2 is the right view of fresh water mussel culture apparatus;
Fig. 3 is the left view of fresh water mussel culture apparatus;
Fig. 4 is that the A-A of Fig. 2 is to cutaway view.
Embodiment
Below in conjunction with accompanying drawing, culture apparatus and the cultural method of laboratory cultures fresh water mussel are set forth, but this culture apparatus and cultural method are in this not conditional intention, in the scope not deviating from, the size of culture apparatus and material can have multiple variation and modification.
Embodiment one: as Figure 1-4, the laboratory fresh water mussel culture apparatus of present embodiment is comprised of culture pond 1, internal partition and adsorbent equipment 2, wherein:
Described culture pond 1 is cuboid, and length and width are high to be respectively: 1.2m, 1m, 0.5m, and the material of whole culture pond is certain rigid material, the inner surface of culture pond need be polished coarse the adhering to of fresh water mussel that be beneficial to.The left side wall of culture pond is provided with a water inlet 3, and water inlet 3 is for providing fresh water mussel grow required moisture and nutriment, and water inlet 3 is connected with water inlet pipe; The right side wall of culture pond is provided with a delivery port 4, and delivery port 4 is for discharging the metabolite of fresh water mussel and being deposited on the fresh water mussel of dying in culture pond bottom, and delivery port 4 is connected with outlet pipe, and the pipe diameter of water inlet pipe and outlet pipe is 0.1m.
Described internal partition is comprised of front dividing plate 5 and rear bulkhead 6, and front dividing plate 5 and rear bulkhead 6 alternative arrangements form revolution gallery type, have some current and turn and dead angle, can better simulate actual aqueduct.The revolution gallery form of whole device is to realize by controlling the distance of dividing plate and culture apparatus front and rear wall, and the front end of front dividing plate 5 is close to the front side wall of culture pond 1, and rear end is apart from culture pond 1 rear wall 0.1m; The rear wall of culture pond 1 is close in the rear end of rear bulkhead 6, and front end is apart from culture pond 1 antetheca 0.1m; Distance between front dividing plate 5 and rear bulkhead 6 is 0.2m.
Take Fig. 1 as example, and internal partition is comprised of three front dividing plates 5 and two rear bulkheads 6, and first and third, five is that front dividing plate 5, the second, four are rear bulkhead 6, between front dividing plate 5 and rear bulkhead 6, is provided with adsorbent equipment 2.Internal partition is 0.06m apart from culture pond 1 upper and lower surface.
Described adsorbent equipment 2 is comprised of several adsorption plates 7, and adsorption plate 7 is fixed on by iron wire 8 in the middle of the gallery of front dividing plate 5 and rear bulkhead 6 compositions, and the top and bottom of adsorption plate 7 are 1/3 of culture pond 1 height apart from the distance of pond Di He Chi Ding respectively.Adsorption plate 7 is multi-disc, hangs on side by side on iron wire 8, can conveniently take out.Adsorbent equipment 2 is 0.1m with the distance of internal partition.
Utilize above-mentioned culture apparatus to carry out the cultivation of fresh water mussel, whole incubation need be carried out under lucifuge condition, and concrete fostering requirement is as follows:
1, the nutriment of fresh water mussel can be algae, algae concentration need be controlled at 2.5mg/L left and right, algae concentration is too low can not give the growth needs that meets fresh water mussel, algae excessive concentration, can cause body eutrophication, although have a large amount of algae substances as food source, Dissolved Oxygen in Water content is low, also restricted the growth of fresh water mussel.The nutriment of fresh water mussel also can be various nanoplanktons and some organic debris.
2, laboratory need guarantee well-ventilated, and oxygen in water is controlled at about 3mg/L, if oxygen in water concentration lower than 2.2mg/L, fresh water mussel can, because of anoxic mortality, consider that from economic angle oxygen in water concentration is unsuitable too high.
3, water temperature need be controlled at 22 ± 2 ℃, and under this temperature condition, fresh water mussel physiological activity ability is strong, and growth rapidly, during water temperature lower (15 ℃ of left and right), fresh water mussel physiological activity is very weak, seldom, water temperature is higher than 30 ℃ for secretion, the meeting of fresh water mussel because of metabolism restricted and dead.Control temperature by air-conditioning summer, places heating rod winter in water body.
4, water volume flow rate need be controlled at 0.1 ~ 0.2m/s, when flow rate of water flow is too low, can not guarantee sufficient food source, and growing of fresh water mussel is restricted; When flow velocity is too high, a large amount of fresh water mussels have little time wall and are taken away this section, even if having an opportunity to be attached to wall, its byssus intensity is also difficult to resist washing away of high-velocity flow.
5, water body pH need be controlled at 6.5 ~ 9, and fresh water mussel is reduced to below 6.5 in 6.5 ~ 9, pH value the scope of restraining oneself of pH value, or is elevated to 9 and can threatens to the existence of fresh water mussel when above, and water pH value is too high or too low also can cause damage to culture apparatus.
6, the water in culture apparatus is changed once to get rid of the metabolite of fresh water mussel for every 10 ~ 14 days, if the dead bottom that can be deposited on culture apparatus of fresh water mussel, if get rid of not in time, can produce rancid, only need utilize in such cases current scour effect by the individual discharge of death, then flushing pipe number can be removed peculiar smell for several times.Embodiment two: what present embodiment was different from embodiment one is, the material of described culture pond 1 is lucite, the inner surface of culture pond 1 need be polished coarse the adhering to of fresh water mussel that be beneficial to, and by the surface that utilizes millboard or tarpaulin to cover whole culture pond 1, guarantees lucifuge condition.
Embodiment three: present embodiment is different from embodiment one, two, and the material of described culture pond 1 is concrete, culture pond 1 upper surface covering millboard or tarpaulin are to guarantee lucifuge condition.
Embodiment four: present embodiment is different from embodiment one, two, three, and the material of described culture pond 1 is iron plate, culture pond 1 upper surface covering millboard or tarpaulin are to guarantee lucifuge condition.
Embodiment five: what present embodiment was different from embodiment one, two, three, four is, on the number of described adsorbent equipment 2 and each adsorbent equipment, the number of adsorption plate 7, according to the number adjustment of fresh water mussel, guarantee that fresh water mussel can obtain sufficient oxygen and nutriment in process of growth.
Embodiment six: present embodiment is different from embodiment one, two, three, four, five, described adsorbent equipment 2 can be reduced to coarse cloth, bamboo raft, protection network etc., also can be designed to the rigid plate in tool hole.
Claims (10)
1. laboratory fresh water mussel culture apparatus, is characterized in that described culture apparatus is comprised of culture pond (1), internal partition and adsorbent equipment (2); The left side wall of culture pond (1) is provided with water inlet (3), and right side wall is provided with delivery port (4), and the inside of culture pond (1) is provided with internal partition and the adsorbent equipment (2) that is parallel to left and right sidewall; Described internal partition is comprised of front dividing plate (5) and rear bulkhead (6), the front end of front dividing plate (5) is close to the antetheca of culture pond (1), the rear wall of culture pond (1) is close in the rear end of rear bulkhead (6), front dividing plate (5) and rear bulkhead (6) alternative arrangement form revolution gallery type, between front dividing plate (5) and rear bulkhead (6), are provided with adsorbent equipment (2).
2. laboratory according to claim 1 fresh water mussel culture apparatus, it is characterized in that described adsorbent equipment (2) is comprised of several adsorption plates (7), adsorption plate (7) is fixed in the middle of the gallery of front dividing plate (5) and rear bulkhead (6) composition by iron wire (8).
3. laboratory according to claim 1 fresh water mussel culture apparatus, is characterized in that described adsorption plate (7), for multi-disc, hangs on iron wire (8) side by side.
4. laboratory according to claim 1 fresh water mussel culture apparatus, the material that it is characterized in that described culture pond (1) is lucite, concrete or iron plate.
5. laboratory according to claim 1 fresh water mussel culture apparatus, is characterized in that described adsorbent equipment (2) is for the rigid plate in coarse cloth, bamboo raft, protection network or tool hole.
6. laboratory according to claim 1 fresh water mussel culture apparatus, is characterized in that described culture pond (1) is cuboid, and length and width are high to be respectively: 1.2m, 1m, 0.5m; Internal partition is 0.06m apart from culture pond (1) upper and lower surface, and the rear end of front dividing plate (5) is apart from culture pond (1) rear wall 0.1m; The front end of rear bulkhead (6) is apart from culture pond (1) antetheca 0.1m; Distance between front dividing plate (5) and rear bulkhead (6) is 0.2m; The top and bottom of adsorbent equipment (2) are 1/3 of culture pond (1) height apart from the distance of pond Di He Chi Ding respectively, and adsorbent equipment (2) is 0.1m with the distance of internal partition.
7. one kind is utilized the method that laboratory fresh water mussel culture apparatus is cultivated fresh water mussel described in the arbitrary right of claim 1-6, it is characterized in that described incubation carries out under lucifuge condition, at cultivation stage, add nutriment every day in culture pond, keep water temperature at 22 ± 2 ℃, flow control is at 0.1 ~ 0.2m/s in culture pond for former water, and oxygen in water concentration is 2.2 ~ 3mg/L, water body pH is controlled at 6.5 ~ 9, and the water in culture pond is changed once for every 10 ~ 14 days.
8. the cultural method of laboratory according to claim 7 fresh water mussel, is characterized in that described nutriment is algae, nanoplankton or organic debris.
9. the cultural method of laboratory according to claim 8 fresh water mussel, is characterized in that the concentration of described algae is controlled at 2.5mg/L.
10. the cultural method of laboratory according to claim 7 fresh water mussel, is characterized in that described oxygen in water concentration is 3mg/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310543654.4A CN103548665B (en) | 2013-11-06 | 2013-11-06 | Laboratory fresh water mussel culture apparatus and utilize it to cultivate the method for fresh water mussel |
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| CN201310543654.4A CN103548665B (en) | 2013-11-06 | 2013-11-06 | Laboratory fresh water mussel culture apparatus and utilize it to cultivate the method for fresh water mussel |
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| CN103548665A true CN103548665A (en) | 2014-02-05 |
| CN103548665B CN103548665B (en) | 2015-08-05 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105110435A (en) * | 2015-09-15 | 2015-12-02 | 清华大学 | Limnopernafortunei killing pool device and killing method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107711304B (en) * | 2017-10-31 | 2019-09-13 | 四川明峰农业开发有限公司 | One plant stereo cultivation apparatus |
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| CN103548665B (en) | 2015-08-05 |
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