CN103548652B - Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings - Google Patents
Rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings Download PDFInfo
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Abstract
The invention discloses a rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings. The rapid tree cultivation method is to bind dendrobium candidum tissue-culture bottle seedlings after subjected to short-term refining on threes for cultivation, and includes seedling hardening, seedling fetching, pretreatment of seedlings, treatment on tree bark and moss, preparation of a binding material, binding of the seedlings, spraying sanitization, spraying management and final-period management. According to the invention, the tree cultivation method is simplified, seedling hardening and tree cultivation effects are optimized, production period is shortened, relatively high seedling survival rate is guaranteed as the survival rate is above 0.095, the obtained medicine is high in quality, multiple effects of speediness, high yield and excellent production are achieved, and the rapid tree cultivation method for dendrobium candidum tissue-culture bottle seedlings is an efficient and feasible tree cultivation method for dendrobium candidum.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of quick tree culture method of candidum tissue culturing bottle seedling.
Background technology
Dendrobium candidum is one of most precious traditional Chinese medicine of China, and be classified as first of " Chinese nine immortals grass " by honor, have the laudatory title of " in medicine gold ", international medicinal plant circle is called " medicine circle giant panda ".The primary medicinal component of dendrobium candidum has the trace element etc. of polysaccharide, flavones, alkaloid, amino acid, phenols and tens kinds of human body requirements.The iron-sheet dendrobe fresh product of current per kilogram can sell for more than thousand yuan.The imitative wild artificial planting stem of noble dendrobium, the stem of noble dendrobium effective component obtained than greenhouse gardening is higher, and the market price also exceeds more than three times than common dendrobium candidum.Because plantation is on trunk, upper and lower three-dimensional plantation improves output.But dendrobium candidum seedling plantlet in vitro needs to tame half a year more than through booth, front " climbing the tree ", also needs the sylvan life vegetative period through four to six months, can be transplanted on tree and carry out imitating wild planting.In prior art common way be annual May ~ November, by dendrobium candidum seedling first by container nursery, be planted in the shade net of booth, tide over domestication; After six months, duration November ~ March, start to move on to sylvan life, select again afterwards cool place, moistening place evergreen broad-leaved forest or have the plantation ' place ' of liver moss.Increase the input of human resources and cost so undoubtedly.
CN102499038A discloses a kind of tree culture method of dendrobium candidum, is to select the veined seeds of bark surface to be the tree that grows nonparasitically upon another plant, and select the dendrobium candidum seedling through taming to be seedling of growing nonparasitically upon another plant, selection straw rope is tied rope; Seedling of growing nonparasitically upon another plant is placed in the trunk surface of the tree that grows nonparasitically upon another plant, is wound around a few in the root place tied rope of seedling of growing nonparasitically upon another plant and is fixed; Tree training humid control is at 70-85%.The problem that the program solves realizes planting dendrobium candidum on the veined seeds of all surface, and its deficiency is that between seedling, line-spacing is unreasonable, and spacing in the rows is failed to understand, wastes plantation space; Before seedling is climbed the tree, lack pretreatment, measure deficiency of climbing the tree, cause the growth of dendrobium candidum seedling comparatively slow, the quality of the medicinal material of acquisition is not ideal enough.
And for example a kind of Tie-to-tree dendrobium officinale cultivation method disclosed in CN102823401A, be=comprise the training of seedling group to breed, training tissue culture seedling, domestication seedling ties up tree cultivation, its Special attention will be given to be hardening matrix, hardening fertilizer, domestication seedling, cultivation forest land, tie up the selection of tree seeds, and tie up seedling method (will clean and the moss of sterilizing be attached to domestication seedling root, with the rope made of hemp of sterilizing or Cortex Cunninghamiae Lanceolatae, domestication seedling is tied up on trunk, the rope made of hemp or Cortex Cunninghamiae Lanceolatae tie up the root at domestication seedling, every 4 ~ 7 strain domestication seedlings are one clump), water spray opportunity and ambient humidity requirement is emphasized in cultivation management method.The deficiency of the program is, the domestication time of seedling is longer (18 ~ 30 months), and the production cycle is long, and operating process is comparatively complicated.
Summary of the invention
The object of the present invention is to provide a kind of quick tree culture method of candidum tissue culturing bottle seedling, solve that the tree of dendrobium candidum in the prior art culture method domestication time is long, the production cycle grow, yield poorly, measure the is not enough problem causing medical material quanlity undesirable.
A quick tree culture method for candidum tissue culturing bottle seedling, is cultivated to tree is upper by bondage after refinery by candidum tissue culturing bottle seedling, comprises the following steps:
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 10 ~ 20 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 24 ~ 48h;
(2) pretreatment of seedling:
Treat that seedling that step (1) gained drains is sterilized and to dry in the air to root in white, shoot root portion is put to field planting water, soaks 25 ~ 35min, then pull out, drain away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰ ~ 1.2 ‰, sodium onitrophenol 1.8 ‰ ~ 2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰ ~ 2.2 ‰, thiamine hydrochloride 99 ‰ ~ 101 ‰, puridoxine hydrochloride 4.8 ‰ ~ 5.2 ‰, inositol 99 ‰ ~ 101 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry after just being returned by liver moss, puts into thimerosal and soak 5 ~ 10min, pull out, firmly squeezing, and loose being open in clean plane is dried, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6 ~ 8cm, long 3 ~ 4m;
(5) bondage of seedling:
Side, dendrobium candidum shoot root portion is encased with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 4 ~ 6cm, carry out bondage with the binding material in step (4) again, tie after 2 circles, then successively bondage, line-spacing 15 ~ 20cm, the bondage on every tree is highly 4 ~ 6m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 4 ~ 6 days;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰ ~ 1.2 ‰, sodium onitrophenol 1.8 ‰ ~ 2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰ ~ 2.2 ‰, thiamine hydrochloride 99 ‰ ~ 101 ‰, puridoxine hydrochloride 4.8 ‰ ~ 5.2 ‰, inositol 99 ‰ ~ 101 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
The composition of the water of field planting described in the present invention is: Sodium p-nitrophenoxide 0.9 ‰ ~ 1.1 ‰, sodium onitrophenol 1.9 ‰ ~ 2.1 ‰, 5-nitroguaiacol sodium salt 1.9 ‰ ~ 2.1 ‰, thiamine hydrochloride 99.5 ‰ ~ 100.5 ‰, puridoxine hydrochloride 4.9 ‰ ~ 5.1 ‰, inositol 99.5 ‰ ~ 100.5 ‰, all the other are water.
The composition of the water of field planting described in the present invention is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, all the other are water.
The hardening time in step of the present invention (1) is 14 ~ 16 days.
In step of the present invention (2), shoot root portion is put and soak 29 ~ 31min to field planting water.
In step of the present invention (3), liver moss is put into thimerosal and soak 7 ~ 8min.
In step of the present invention (5), the spacing in the rows of dendrobium candidum seedling is 5cm, and line-spacing is 17 ~ 18cm.
In step of the present invention (4), sunshade net is cut into the binding material of wide 7cm, long 3.5m.
Beneficial effect of the present invention: simplify tree culture method, optimizes hardening and tree training effect, shortens the production cycle, ensure that higher seedling survival rate, the medical material quanlity obtained is superior, reaches quick, high yield, excellent product multiple effect, is a kind of efficiently easy dendrobium candidum tree culture method.The present invention coordinates specific seedling processing method by glass sunlight house short time hardening, compared with booth conventional in prior art is tamed and planted again half a year, greatly can shorten the dendrobium candidum seedling domestication time to external world; By specific bondage technology, coordinate the use of field planting water, the survival rate of dendrobium candidum seedling can be improved, the manpower, the financial resources that significantly reduce dendrobium candidum plantation drop into, its cost is about about 1/3rd of traditional growing dendrobium cost, and being easy to operation, generalization is strong, does a great deal of good to drive medicinal herb grower economic development.Adopt the inventive method plantation candidum tissue culturing bottle seedling, survival rate is to more than 95 ‰; Substantially increase dendrobium candidum output, shorten the production cycle; Dendrobium candidum annual value of production on every tree can tens thousand of unit, and the flower of dendrobium candidum (the making tea bubble drink) benefit that also can produce 2 ~ 30,000 yuan per jin; The dendrobium candidum plant pedicel of final acquisition is full, black joint is obvious, and there is formae oxydatae on surface, and diameter is 0.6 ~ 0.8cm about, be about 15 ~ 20cm, frangibility, section is colloid, and sticky mouth is chewed in life, chew rear few slag, fresh goods functional component is high, remains the competent cell of dendrobium candidum to greatest extent, tool green health health effect; Made golden yellow, glossy, the thin longitudinal grin of tool in dry product medicinal material surface, quality is pliable and tough and real, and section is more smooth, and quality is superior.
The present invention adopts sunshade net to substitute tied rope, can play good to be in the light, to keep off the rain, moisturizing, thermoregulatory effect, and light weight, good permeability, long service life, is better than common tied rope.The present invention adopts pretreated liver moss and bark to be more suitable for dendrobium candidum seedling rooting, and reduce the probability that virus evil occurs to a certain extent, thimerosal can select the Common Disinfectant Solution such as carbendazim, tpn liquid.Bottle cap is unscrewed placement one evening by the present invention after hardening, and second day opens bottle cap, can promote the gas exchanges inside and outside bottle, strengthens the refinery effect of seedling.The present invention uses the field planting water of specific proportioning can obtain beyond thought effect, not only improves the survival rate of dendrobium candidum seedling in the shortening refinery time at the same time, also improves the quality of dendrobium candidum plant.The present invention specify that the time range be immersed in shoot root portion in field planting water, can save the production time, be conducive to enhancing productivity under in order to be effective prerequisite, shortens the production cycle.In the present invention the width of binding material and length specific, spacing in the rows during bondage dendrobium candidum seedling and line-spacing specific, compared with prior art, greatly save plantation space, effectively make use of resource, and achieve good culture effect, improve the yield per unit area of dendrobium candidum.The present invention carries out spraying disinfection and spray field planting water after bondage completes, can effectively prevention and elimination of disease and pests, is conducive to the survival rate and the output that improve dendrobium candidum, is conducive to the quality of lifting dendrobium candidum.
Embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is described.The following stated is preferred embodiment of the present invention, can be used for explaining and supporting the present invention, but any pro forma restriction is not done to the present invention, anyly do not depart from technical solution of the present invention content, and simple modification following examples to be done according to technical spirit of the present invention, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Embodiment 1
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 10 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 24h;
(2) pretreatment of seedling:
Treat that seedling that step (1) gained drains is sterilized and to dry in the air to root in white, shoot root portion is put to field planting water, soaks 25min, then pull out, drain away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰, sodium onitrophenol 1.8 ‰, 5-nitroguaiacol sodium salt 1.8 ‰, thiamine hydrochloride 99 ‰, puridoxine hydrochloride 4.8 ‰, inositol 99 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry, put into thimerosal and soak 5min, pull out after just being returned by liver moss, firmly squeezing, and loose being open in clean plane is dried, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6cm, long 3m;
(5) bondage of seedling:
Encase side, dendrobium candidum shoot root portion with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 4cm, then carry out bondage with the binding material in step (4), tie after 2 circles, then successively bondage, line-spacing 15cm, the bondage on every tree is highly 4m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 4 days;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰, sodium onitrophenol 1.8 ‰, 5-nitroguaiacol sodium salt 1.8 ‰, thiamine hydrochloride 99 ‰, puridoxine hydrochloride 4.8 ‰, inositol 99 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 2
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 12 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 30h;
(2) pretreatment of seedling:
Treat that seedling that step (1) gained drains is sterilized and to dry in the air to root in white, shoot root portion is put to field planting water, soaks 27min, then pull out, drain away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 1.2 ‰, sodium onitrophenol 2.2 ‰, 5-nitroguaiacol sodium salt 2.2 ‰, thiamine hydrochloride 101 ‰, puridoxine hydrochloride 5.2 ‰, inositol 101 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry, put into thimerosal and soak 6min, pull out after just being returned by liver moss, firmly squeezing, and loose being open in clean plane is dried, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6.5cm, long 3.5m;
(5) bondage of seedling:
Encase side, dendrobium candidum shoot root portion with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 4.5cm, then carry out bondage with the binding material in step (4), tie after 2 circles, then successively bondage, line-spacing 16cm, the bondage on every tree is highly 4.5m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 5 days;
Described field planting water constituent is: Sodium p-nitrophenoxide 1.2 ‰, sodium onitrophenol 2.2 ‰, 5-nitroguaiacol sodium salt 2.2 ‰, thiamine hydrochloride 101 ‰, puridoxine hydrochloride 5.2 ‰, inositol 101 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 3
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 14 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 36h;
(2) pretreatment of seedling:
Treat that seedling that step (1) gained drains is sterilized and to dry in the air to root in white, shoot root portion is put to field planting water, soaks 29min, then pull out, drain away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 0.9 ‰, sodium onitrophenol 1.9 ‰, 5-nitroguaiacol sodium salt 1.9 ‰, thiamine hydrochloride 99.5 ‰, puridoxine hydrochloride 4.9 ~ 5.1 ‰, inositol 99.5 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry, put into thimerosal and soak 7min, pull out after just being returned by liver moss, firmly squeezing, and loose being open in clean plane is dried, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 7cm, long 3.5m;
(5) bondage of seedling:
Encase side, dendrobium candidum shoot root portion with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 5cm, then carry out bondage with the binding material in step (4), tie after 2 circles, then successively bondage, line-spacing 17cm, the bondage on every tree is highly 5m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 6 days;
The composition of described field planting water is: Sodium p-nitrophenoxide 0.9 ‰, sodium onitrophenol 1.9 ‰, 5-nitroguaiacol sodium salt 1.9 ‰, thiamine hydrochloride 99.5 ‰, puridoxine hydrochloride 4.9 ‰, inositol 99.5 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 4
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 16 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 42h;
(2) pretreatment of seedling:
Treat that seedling that step (1) gained drains is sterilized and to dry in the air to root in white, shoot root portion is put to field planting water, soaks 31min, then pull out, drain away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.1 ‰, sodium onitrophenol 2.1 ‰, 5-nitroguaiacol sodium salt 2.1 ‰, thiamine hydrochloride 100.5 ‰, puridoxine hydrochloride 5.1 ‰, inositol 100.5 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry, put into thimerosal and soak 8min, pull out after just being returned by liver moss, firmly squeezing, and loose being open in clean plane is dried, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 7.5cm, long 3.5m;
(5) bondage of seedling:
Encase side, dendrobium candidum shoot root portion with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 5.5cm, then carry out bondage with the binding material in step (4), tie after 2 circles, then successively bondage, line-spacing 18cm, the bondage on every tree is highly 5.5m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 4 days;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.1 ‰, sodium onitrophenol 2.1 ‰, 5-nitroguaiacol sodium salt 2.1 ‰, thiamine hydrochloride 100.5 ‰, puridoxine hydrochloride 5.1 ‰, inositol 100.5 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 5
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 18 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 48h;
(2) pretreatment of seedling:
Treat that seedling that step (1) gained drains is sterilized and to dry in the air to root in white, shoot root portion is put to field planting water, soaks 33min, then pull out, drain away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.1 ‰, sodium onitrophenol 2.1 ‰, 5-nitroguaiacol sodium salt 2.1 ‰, thiamine hydrochloride 100.5 ‰, puridoxine hydrochloride 5.1 ‰, inositol 100.5 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry, put into thimerosal and soak 9min, pull out after just being returned by liver moss, firmly squeezing, and loose being open in clean plane is dried, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 8cm, long 4m;
(5) bondage of seedling:
Encase side, dendrobium candidum shoot root portion with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 6cm, then carry out bondage with the binding material in step (4), tie after 2 circles, then successively bondage, line-spacing 19cm, the bondage on every tree is highly 6m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 5 days;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Embodiment 6
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 20 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 36h;
(2) pretreatment of seedling:
Treat that seedling that step (1) gained drains is sterilized and to dry in the air to root in white, shoot root portion is put to field planting water, soaks 35min, then pull out, drain away the water;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry, put into thimerosal and soak 10min, pull out after just being returned by liver moss, firmly squeezing, and loose being open in clean plane is dried, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 7cm, long 4m;
(5) bondage of seedling:
Encase side, dendrobium candidum shoot root portion with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 5cm, then carry out bondage with the binding material in step (4), tie after 2 circles, then successively bondage, line-spacing 20cm, the bondage on every tree is highly 5m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 6 days;
The composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
Claims (8)
1. a quick tree culture method for candidum tissue culturing bottle seedling, is cultivated to tree is upper by bondage after hardening by candidum tissue culturing bottle seedling, it is characterized in that: comprise the following steps:
(1) hardening and get seedling:
The whole bottle of candidum tissue culturing bottle seedling was moved to glass sunlight house hardening after 10 ~ 18 days in culturing room, bottle cap is unscrewed placement one evening, bottle cap is opened by second day, is pressed from both sides out by seedling from bottle with tweezers, and rinsed clean is pulled out and drained 24 ~ 48h;
(2) pretreatment of seedling:
Treat that the seedling that step (1) gained drains is sterilized and dries in the air to root in white, shoot root portion is placed in field planting water and soaks 25 ~ 35min, then pull out, drain away the water;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰ ~ 1.2 ‰, sodium onitrophenol 1.8 ‰ ~ 2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰ ~ 2.2 ‰, thiamine hydrochloride 99 ‰ ~ 101 ‰, puridoxine hydrochloride 4.8 ‰ ~ 5.2 ‰, inositol 99 ‰ ~ 101 ‰, and all the other are water;
(3) process of bark and liver moss:
Suitably processed by bark superficial, removing impurity and branch, sterilize for subsequent use, clean up, dry after being adopted back by liver moss, puts into thimerosal and soak 5 ~ 10min, pull out, firmly squeezing, scatters to place in clean plane and dry, for subsequent use;
(4) preparation of binding material:
Sunshade net is cut into the binding material of wide 6 ~ 8cm, long 3 ~ 4m;
(5) bondage of seedling:
Side, dendrobium candidum shoot root portion is encased with liver moss, expose foundation portion, the opposite side do not encased by liver moss is affixed to bark superficial, spacing in the rows 4 ~ 6cm, carry out bondage with the binding material in step (4) again, tie after 2 circles, then successively bondage, line-spacing 15 ~ 20cm, the bondage on every tree is highly 4 ~ 6m;
(6) spraying disinfection, spray management:
Carry out spraying disinfection once in 48h after bondage completes, spray a field planting water every 4 ~ 6 days;
Described field planting water constituent is: Sodium p-nitrophenoxide 0.8 ‰ ~ 1.2 ‰, sodium onitrophenol 1.8 ‰ ~ 2.2 ‰, 5-nitroguaiacol sodium salt 1.8 ‰ ~ 2.2 ‰, thiamine hydrochloride 99 ‰ ~ 101 ‰, puridoxine hydrochloride 4.8 ‰ ~ 5.2 ‰, inositol 99 ‰ ~ 101 ‰, and all the other are water;
(7) final-period management:
Final-period management operates routinely, prevention and elimination of disease and pests, and humidity adjustment is carried out according to a conventional method.
2. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, it is characterized in that: the composition of described field planting water is: Sodium p-nitrophenoxide 0.9 ‰ ~ 1.1 ‰, sodium onitrophenol 1.9 ‰ ~ 2.1 ‰, 5-nitroguaiacol sodium salt 1.9 ‰ ~ 2.1 ‰, thiamine hydrochloride 99.5 ‰ ~ 100.5 ‰, puridoxine hydrochloride 4.9 ‰ ~ 5.1 ‰, inositol 99.5 ‰ ~ 100.5 ‰, all the other are water.
3. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, it is characterized in that: the composition of described field planting water is: Sodium p-nitrophenoxide 1.0 ‰, sodium onitrophenol 2.0 ‰, 5-nitroguaiacol sodium salt 2.0 ‰, thiamine hydrochloride 100.0 ‰, puridoxine hydrochloride 5.0 ‰, inositol 100.0 ‰, all the other are water.
4. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: the hardening time in described step (1) is 14 ~ 16 days.
5. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: put in shoot root portion in described step (2) and soak 29 ~ 31min to field planting water.
6. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: in described step (3), liver moss is put into thimerosal and soak 7 ~ 8min.
7. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: in described step (5), the spacing in the rows of dendrobium candidum seedling is 5cm, and line-spacing is 17 ~ 18cm.
8. the quick tree culture method of a kind of candidum tissue culturing bottle seedling as claimed in claim 1, is characterized in that: the binding material in described step (4), sunshade net being cut into wide 7cm, long 3.5m.
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| CN106613829A (en) * | 2016-11-16 | 2017-05-10 | 陈勇 | Method for planting dendrobium officinale on tree |
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| CN112056197B (en) * | 2020-09-07 | 2022-04-01 | 贵州沿河乌江生物科技发展有限公司 | Rapid tree culture method for dendrobium officinale tissue culture seedlings |
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