CN103548577A - System and method for producing and culturing liquid spawns of edible fungus - Google Patents
System and method for producing and culturing liquid spawns of edible fungus Download PDFInfo
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- CN103548577A CN103548577A CN201310575209.6A CN201310575209A CN103548577A CN 103548577 A CN103548577 A CN 103548577A CN 201310575209 A CN201310575209 A CN 201310575209A CN 103548577 A CN103548577 A CN 103548577A
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Abstract
The invention discloses a system and a method for producing and culturing liquid spawns of an edible fungus. The system comprises a nutrient solution sterilized tank, a heat exchanger, a cooling-water machine set and a plurality of liquid spawn culture tanks, and further comprises a nutrient solution circulation pipeline, a nutrient solution sterilization and cooling pipeline, a nutrient solution conveying pipeline, a germ-free compressed air inlet pipeline, a blow-off pipeline and a liquid spawn conveying pipeline. The invention further discloses a method for producing and culturing the liquid spawns of the edible fungus by using the system. According to the system and the method provided by the invention, residue spawns in the liquid spawn culture tanks can be used as stock cultures for again culture, so that the infection risks due to successive vaccination can be avoided; the sterilization, the cooling, the conveying and the germ-free air supply of the spawns are completed in inclosed vessels and pipelines, so that the infection probability is zero; the movement of the liquid spawn culture tanks is avoided, so that the investment is reduced, safety and reliability are realized, the amount of used labor is reduced, and the labor intensity is reduced.
Description
Technical field
The present invention relates to making and the culture systems of bacterial classification, particularly a kind of edible fungi liquid strain is made and culture systems; The invention still further relates to and utilize this system to carry out the method that edible fungi liquid strain is made and cultivated.
Background technology
In prior art, it is all to adopt independently equipment that edible fungi liquid strain is made, and its making pattern is:
First strain cultivation tank is carried out to manual cleaning at seeding tank cleaning shop.After cleaning finishes, by manually promoting strain cultivation tank, enter strain cultivation tank weighing area.By manually the culture fluid preparing and clear water being poured in strain cultivation tank.With manually entering seeding tank sterilizer by tank promotion strain cultivation tank, carry out sterilizing again.After sterilizing finishes, by manually under hot conditions, strain cultivation tank being pulled out from seeding tank sterilizer inside, enter seeding tank chilling room, after entering chilling room, first filtrated air pipeline is accessed to the aseptic gas interface of strain cultivation pot bottom, prevent that strain cultivation tank from forming negative pressure in cooling procedure, thereby cause in room gas to enter strain cultivation tank, cause infection.Coolingly by the running water method that stop-spraying is drenched, lowered the temperature, winter, cooling need were about 8 hours.Summer the cooling 10-14 hour that needs.
After cooling end, the filtrated air pipeline being connected with the aseptic gas interface of strain cultivation pot bottom is pulled up, by manually promoting strain cultivation tank, enter hundred grades of laminar flow hood belows between seeding tank inoculation again, first interior filtrated air pipeline between seeding tank inoculation is accessed to the aseptic gas interface of 3 strain cultivation pot bottom, make to form malleation in tank, protect from infection.Afterwards under hundred grades of laminar flow hood by manually opening inoculation quick-actuating closure, mother is planted to access and completes inoculation in tank.
After having inoculated, first the filtrated air pipeline being connected with the aseptic gas interface of strain cultivation pot bottom is pulled up, by manually promoting strain cultivation tank, enter between strain cultivation again, after entering, by the aseptic gas interface of filtrated air pipeline access strain cultivation pot bottom between strain cultivation, to mycelia oxygen supply in tank.Cultivate one-period after 7-8 days, bacterial classification output channel is connected with body bacteria culture tank bottom bacterial classification output interface, cultured liquid spawn is flowed to inoculating facility.After inoculation finishes.Empty strain cultivation tank is manually pushed to seeding tank cleaning shop and cleans, recycle.
Mainly there is following defect in this liquid spawn making apparatus of the prior art and operator scheme:
1, working strength is large: because each bacteria culture tank need be installed castor, manually pull carrying, so gross weight can only be below one ton, or manpower is difficult to drive;
2, infection rate is high: each seeding tank needs to patch, dismantle filtrated air joint for 3 times, patches bacterial classification output channel joint for 1 time, successively opens the inoculation of inoculation quick-actuating closure in a cultivation cycle.Above process all must complete under aseptic condition, and requires employee's technical ability very skilled, otherwise very easily causes infection;
3, cost of investment is high.By every day, by 3 tanks, cultivation cycle, within 8 days, calculate.Separately add 3 non-conservation tanks, amount to 3 * 8+3=27 tank.Sterilizing need be equipped with sterilizer special;
4, water consumption is large, when cooling running water not stop-spraying drench seeding tank, trickling, can not recycle in vain, causes waste.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, provides a kind of reasonable in design, edible fungi liquid strain safe, that greatly reduce infection rate easy to use to make and culture systems.
Another technical problem to be solved by this invention is to provide application said system and carries out the method that edible fungi liquid strain is made and cultivated.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is that a kind of edible fungi liquid strain is made and culture systems, is characterized in: this system comprises nutrient solution sterilization tank, heat exchanger, handpiece Water Chilling Units and some strain cultivation tanks; And,
Nutrient solution circulation line: its one end is connected to the top of nutrient solution sterilization tank, the other end is connected to the bottom of nutrient solution sterilization tank; This pipeline is provided with circulating pump, and this pipeline access heat exchanger pick out ，Gai road and be connected with into steam pipework from carrying hot device;
On nutrient solution sterilization tank or nutrient solution circulation line, be connected with inlet pipeline;
Nutrient solution sterilizing and cooling line: its one end is connected with the water inlet of handpiece Water Chilling Units
,the other end is connected with the delivery port of handpiece Water Chilling Units; This pipeline is provided with coolant pump, and this pipeline access heat exchanger pick out ，Gai road and be connected with into steam pipework and hydrophobic exhaust line from carrying hot device;
Nutrition pipeline road: its outlet is connected to the bottom of nutrient solution sterilization tank, import is connected with the entrance of each strain cultivation tank;
Enter aseptic compressed gas pipeline: it is connected with the top of nutrient solution sterilization tank, the bottom of each strain cultivation tank respectively;
Blowoff line: its entrance point is connected with the bottom of each strain cultivation tank respectively, and discharges by sewage draining exit;
Liquid spawn transfer pipeline: its entrance point is connected with each strain cultivation pot bottom respectively, and exports by liquid spawn delivery outlet.
In system of the present invention, further preferred technical scheme is:
1. the heat exchanger described in is heat-exchangers of the plate type;
2. on nutrient solution sterilization tank, be provided with liquid level gauge and temperature sensor;
3. in nutrient solution sterilization tank bottom, be provided with and be provided with bilge discharge pipe road;
4. on liquid spawn transfer pipeline, be connected with into steam pipework and inlet pipeline;
5. on nutrition pipeline road, be connected with inlet pipeline;
6. entering on aseptic compressed gas pipeline to be connected with pressure relief pipe;
7. in strain cultivation tank, bottom is provided with agitator;
8. the agitator described in is preferably agitator air, and described agitator air comprises stirring back shaft and establishes puddler thereon, the pore group that is each side provided with at least one group of perforate opposite direction of puddler.
In system of the present invention, on each pipeline, conventional valve is set on demand or instrument is controlled.Between each pipeline, according to arranging conveniently, can adopt the mode that shares one section of pipeline to settle.
Technical problem to be solved by this invention is to realize by following technical scheme.The system described in a kind of any one that use in above technical scheme of the invention also discloses is carried out bacterial classification and is made and the method for cultivating, and is characterized in, its step is as follows:
(1) without bacterial nutrient solution, make:
A. add water: prepare in proportion nutrient solution, and the charge door by nutrient solution sterilization tank adds in tank body by it, start on nutrient solution sterilization tank or nutrient solution circulation line and be connected with inlet pipeline, add running water quantitative to adjust concentration;
B. heat up: start nutrient solution circulation line, will in nutrition liquid pump heat exchanger, circulate; Nutrient solution sterilizing simultaneously and cooling line, the nutrient solution in heat exchanger heats up; Simultaneously by hydrophobic exhaust line excretion steam condensate (SC);
C. sterilizing: when nutrient solution intensification reaches setting value, carry out the sterilizing of nutrient solution constant temperature under 100-130 degree Celsius; The amount of entering steam during intensification sterilizing is controlled by temperature, during lower than setting value, opens, and during higher than setting value, closes;
D. it is cooling: after sterilizing finishes, steam off pipeline, is pumped into the cold water of handpiece Water Chilling Units in heat exchanger and circulates by nutrient solution sterilizing and cooling line, and nutrient solution also carries out circulating cooling in heat exchanger simultaneously, when solution temperature is reduced to setting value, cooling completing;
E. aseptic nutrient solution is carried: open nutrition pipeline road and enter aseptic compressed gas pipeline, nutrient solution is delivered to respectively in strain cultivation tank;
F. nutrient solution sterilization tank cleans: open on nutrient solution sterilization tank or nutrient solution circulation line and be connected with inlet pipeline, in tank, inject clear water, carry out wash cycles, heat exchanger and nutrient solution sterilization tank are cleaned;
(2) cultural hypha: nutrient solution enters after strain cultivation tank, under aseptic condition, the inoculation piece of strain cultivation tank is uncapped mother is planted in access tank; During cultivation, open into aseptic compressed gas pipeline, aseptic gas is sent in strain cultivation tank, to mycelia oxygen supply;
When agitator air is housed, the puddler of the agitator air of liquid spawn transfer pipeline is communicated with, and just aseptic gas evenly feeds in tank, and the pore group oppositely arranging can make puddler rotation, realizes and stirring;
(3) liquid spawn output: after cultural hypha completes, open strain cultivation tank, by liquid spawn transfer pipeline, liquid spawn is exported; After output, in tank, remaining part bacterial classification is used when female kind, while injecting new nutrient solution, need not connect new mother's kind again;
(4) strain cultivation tank cleaning and sterilizing: the mother in strain cultivation tank plants the degeneration of considering spawn activity after continuing to use a period of time, mother's kind that need more renew; Renew before female kind, need carry out cleaning and sterilizing to strain cultivation tank, its method is as follows:
A. clean: open liquid spawn transfer pipeline, by the emptying of residual liquid bacterial classification; By nutrition pipeline road, running water is sent into the strain cultivation tank of required cleaning, aseptic gas is sent in tank and cleaned simultaneously, after cleaning, by blowoff line, sewage is discharged;
B. sterilization: after cleaning, by nutrition pipeline road, high-temperature steam is sent in the strain cultivation tank of required sterilizing, carried out sterilizing;
(5) nutrition pipeline road, liquid spawn transfer pipeline cleaning and sterilizing: after each conveying finishes, need carry out cleaning and sterilizing to nutrition pipeline road, liquid spawn transfer pipeline;
A. nutrition pipeline road cleaning and sterilizing: running water is sent into nutrition pipeline road and clean; After cleaning finishes, high-temperature steam is sent into nutrition pipeline road and carry out disinfection;
B. liquid spawn transfer pipeline cleaning and sterilizing: running water is sent into liquid spawn transfer pipeline and clean; After cleaning finishes, high-temperature steam is sent into liquid spawn transfer pipeline and carry out disinfection.
Compared with prior art, the advantage of technical solution of the present invention is mainly reflected in:
1. system of the present invention can complete independently bacterial classification making and cultivation are a kind of production of hybrid seeds equipment of integrated form.
2. the supply of the sterilizing of bacteria culture fluid, cooling, conveying and filtrated air all completes in airtight container and pipeline, and infecting probability is zero.
3. strain cultivation tank, without moving, can customize according to user's consumption every day the specification of tank body.
4. can reduce investment: do not need liquid spawn tank sterilizer.The more former scheme of strain cultivation tank quantity relatively has large batch of minimizing, but the volume of each strain cultivation tank will meet this factory's consumption of a day.
5. liquid spawn tank does not need to clean in principle, general annual cleaning 1-2 time.
6. the remaining bacterial classification in strain cultivation tank can be planted to use as the mother who again cultivates, avoid the successively infection risk of inoculation, greatly reduce and infect probability.
7. system of the present invention can be controlled automatically by computer, safe and reliable, reduces the amount of labour used, has reduced labour intensity.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation of system of the present invention;
Fig. 2 is a kind of plan structure schematic diagram of agitator.
Embodiment
Referring to accompanying drawing, further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Nutrient solution circulation line 4: its one end is connected to the top of nutrient solution sterilization tank 1, the other end is connected to the bottom of nutrient solution sterilization tank 1; This pipeline is provided with circulating pump, and this pipeline access heat exchanger 10 pick out ，Gai road and be connected with into steam pipework from carrying hot device;
On nutrient solution sterilization tank 1 or nutrient solution circulation line 4, be connected with inlet pipeline;
Nutrient solution sterilizing and cooling line 2: its one end is connected with the water inlet of handpiece Water Chilling Units 3
,the other end is connected with the delivery port of handpiece Water Chilling Units 3; This pipeline is provided with coolant pump, and this pipeline access heat exchanger 10 pick out ，Gai road and be connected with into steam pipework and hydrophobic exhaust line from carrying hot device;
Nutrition pipeline road 8: its outlet is connected to the bottom of nutrient solution sterilization tank 1, import is connected with the entrance of each strain cultivation tank 7;
Enter aseptic compressed gas pipeline 5: it is connected with the top of nutrient solution sterilization tank 1, the bottom of each strain cultivation tank 7 respectively;
Blowoff line 9: its entrance point is connected with the bottom of each strain cultivation tank 7 respectively, and discharges by sewage draining exit;
Liquid spawn transfer pipeline 6: its entrance point is connected with each strain cultivation tank 7 bottom respectively, and exports by liquid spawn delivery outlet.
(1) without bacterial nutrient solution, make:
A. add water: prepare in proportion nutrient solution, and the charge door by nutrient solution sterilization tank 1 adds in tank body by it, start on nutrient solution sterilization tank 1 or nutrient solution circulation line 4 and be connected with inlet pipeline, add running water quantitative to adjust concentration;
B. heat up: start nutrient solution circulation line 4, will in nutrition liquid pump heat exchanger 10, circulate; Nutrient solution sterilizing simultaneously and cooling line 2, the nutrient solution in heat exchanger 10 heats up; Simultaneously by hydrophobic exhaust line excretion steam condensate (SC);
C. sterilizing: when nutrient solution intensification reaches setting value, carry out the sterilizing of nutrient solution constant temperature under 100-130 degree Celsius; The amount of entering steam during intensification sterilizing is controlled by temperature, during lower than setting value, opens, and during higher than setting value, closes;
D. it is cooling: after sterilizing finishes, steam off pipeline, is pumped into the cold water of handpiece Water Chilling Units 3 in heat exchanger 10 and circulates by nutrient solution sterilizing and cooling line 2, and nutrient solution also carries out circulating cooling in heat exchanger 10 simultaneously, when solution temperature is reduced to setting value, cooling completing;
E. aseptic nutrient solution is carried: open nutrition pipeline road 8 and enter aseptic compressed gas pipeline 5, nutrient solution is delivered to respectively in strain cultivation tank 7;
F. nutrient solution sterilization tank 1 cleans: open on nutrient solution sterilization tank 1 or nutrient solution circulation line 4 and be connected with inlet pipeline, in tank, inject clear water, carry out wash cycles, heat exchanger 10 and nutrient solution sterilization tank 1 are cleaned;
(2) cultural hypha: nutrient solution enters after strain cultivation tank 7, under aseptic condition, the inoculation piece of strain cultivation tank 7 is uncapped mother is planted in access tank; During cultivation, open into aseptic compressed gas pipeline 5, aseptic gas is sent in strain cultivation tank 7, to mycelia oxygen supply;
When agitator air is housed, the puddler of the agitator air of liquid spawn transfer pipeline 6 is communicated with, and just aseptic gas evenly feeds in tank, and the pore group oppositely arranging can make puddler rotation, realizes and stirring;
(3) liquid spawn output: after cultural hypha completes, open strain cultivation tank 7, by liquid spawn transfer pipeline 6, liquid spawn is exported; After output, in tank, remaining part bacterial classification is used when female kind, while injecting new nutrient solution, need not connect new mother's kind again;
(4) strain cultivation tank 7 cleaning and sterilizings: the mother in strain cultivation tank 7 plants the degeneration of considering spawn activity after continuing to use a period of time, mother's kind that need more renew; Renew before female kind, need carry out cleaning and sterilizing to strain cultivation tank 7, its method is as follows:
A. clean: open liquid spawn transfer pipeline 6, by the emptying of residual liquid bacterial classification; By nutrition pipeline road 8, running water is sent into the strain cultivation tank 7 of required cleaning, aseptic gas is sent in tank and cleaned simultaneously, after cleaning, by blowoff line 9, sewage is discharged;
B. sterilization: after cleaning, by nutrition pipeline road 8, high-temperature steam is sent in the strain cultivation tank 7 of required sterilizing, carried out sterilizing;
(5) nutrition pipeline road 8, liquid spawn transfer pipeline 6 cleaning and sterilizings: after each conveying finishes, need carry out cleaning and sterilizing to nutrition pipeline road 8, liquid spawn transfer pipeline 6;
A. nutrition pipeline road 8 cleaning and sterilizings: running water is sent into nutrition pipeline road 8 and clean; After cleaning finishes, high-temperature steam is sent into nutrition pipeline road 8 and carry out disinfection;
B. liquid spawn transfer pipeline 6 cleaning and sterilizings: running water is sent into liquid spawn transfer pipeline 6 and clean; After cleaning finishes, high-temperature steam is sent into liquid spawn transfer pipeline 6 and carry out disinfection.
Claims (10)
1. edible fungi liquid strain is made and a culture systems, it is characterized in that: this system comprises nutrient solution sterilization tank, heat exchanger, handpiece Water Chilling Units and some strain cultivation tanks; And,
Nutrient solution circulation line: its one end is connected to the top of nutrient solution sterilization tank, the other end is connected to the bottom of nutrient solution sterilization tank; This pipeline is provided with circulating pump, and this pipeline access heat exchanger pick out ，Gai road and be connected with into steam pipework from carrying hot device;
On nutrient solution sterilization tank or nutrient solution circulation line, be connected with inlet pipeline;
Nutrient solution sterilizing and cooling line: its one end is connected with the water inlet of handpiece Water Chilling Units
,the other end is connected with the delivery port of handpiece Water Chilling Units; This pipeline is provided with coolant pump, and this pipeline access heat exchanger pick out ，Gai road and be connected with into steam pipework and hydrophobic exhaust line from carrying hot device;
Nutrition pipeline road: its outlet is connected to the bottom of nutrient solution sterilization tank, import is connected with the entrance of each strain cultivation tank;
Enter aseptic compressed gas pipeline: it is connected with the top of nutrient solution sterilization tank, the bottom of each strain cultivation tank respectively;
Blowoff line: its entrance point is connected with the bottom of each strain cultivation tank respectively, and discharges by sewage draining exit;
Liquid spawn transfer pipeline: its entrance point is connected with each strain cultivation pot bottom respectively, and exports by liquid spawn delivery outlet.
2. system according to claim 1, is characterized in that: described heat exchanger is heat-exchangers of the plate type.
3. system according to claim 1, is characterized in that: on nutrient solution sterilization tank, be provided with liquid level gauge and temperature sensor.
4. system according to claim 1, is characterized in that: in nutrient solution sterilization tank bottom, be provided with and be provided with bilge discharge pipe road.
5. system according to claim 1, is characterized in that: on liquid spawn transfer pipeline, be connected with into steam pipework and inlet pipeline.
6. system according to claim 1, is characterized in that: on nutrition pipeline road, be connected with inlet pipeline.
7. system according to claim 1, is characterized in that: entering on aseptic compressed gas pipeline to be connected with pressure relief pipe.
8. the system described in any one according to claim 1-7, is characterized in that: in strain cultivation tank, bottom is provided with agitator.
9. system according to claim 8, it is characterized in that: described agitator is agitator air, described agitator air comprises stirring back shaft and establishes puddler thereon, the pore group that is each side provided with at least one group of perforate opposite direction of puddler.
10. right to use requires the system of 1-9 described in any one to carry out bacterial classification to make and a method of cultivating, it is characterized in that, its step is as follows:
(1) without bacterial nutrient solution, make:
A. add water: prepare in proportion nutrient solution, and the charge door by nutrient solution sterilization tank adds in tank body by it, start on nutrient solution sterilization tank or nutrient solution circulation line and be connected with inlet pipeline, add running water quantitative to adjust concentration;
B. heat up: start nutrient solution circulation line, will in nutrition liquid pump heat exchanger, circulate; Nutrient solution sterilizing simultaneously and cooling line, the nutrient solution in heat exchanger heats up; Simultaneously by hydrophobic exhaust line excretion steam condensate (SC);
C. sterilizing: when nutrient solution intensification reaches setting value, carry out the sterilizing of nutrient solution constant temperature under 100-130 degree Celsius; The amount of entering steam during intensification sterilizing is controlled by temperature, during lower than setting value, opens, and during higher than setting value, closes;
D. it is cooling: after sterilizing finishes, steam off pipeline, is pumped into the cold water of handpiece Water Chilling Units in heat exchanger and circulates by nutrient solution sterilizing and cooling line, and nutrient solution also carries out circulating cooling in heat exchanger simultaneously, when solution temperature is reduced to setting value, cooling completing;
E. aseptic nutrient solution is carried: open nutrition pipeline road and enter aseptic compressed gas pipeline, nutrient solution is delivered to respectively in strain cultivation tank;
F. nutrient solution sterilization tank cleans: open on nutrient solution sterilization tank or nutrient solution circulation line and be connected with inlet pipeline, in tank, inject clear water, carry out wash cycles, heat exchanger and nutrient solution sterilization tank are cleaned;
(2) cultural hypha: nutrient solution enters after strain cultivation tank, under aseptic condition, the inoculation piece of strain cultivation tank is uncapped mother is planted in access tank; During cultivation, open into aseptic compressed gas pipeline, aseptic gas is sent in strain cultivation tank, to mycelia oxygen supply;
When agitator air is housed, the puddler of the agitator air of liquid spawn transfer pipeline is communicated with, and just aseptic gas evenly feeds in tank, and the pore group oppositely arranging can make puddler rotation, realizes and stirring;
(3) liquid spawn output: after cultural hypha completes, open strain cultivation tank, by liquid spawn transfer pipeline, liquid spawn is exported; After output, in tank, remaining part bacterial classification is used when female kind, while injecting new nutrient solution, need not connect new mother's kind again;
(4) strain cultivation tank cleaning and sterilizing: the mother in strain cultivation tank plants the degeneration of considering spawn activity after continuing to use a period of time, mother's kind that need more renew; Renew before female kind, need carry out cleaning and sterilizing to strain cultivation tank, its method is as follows:
A. clean: open liquid spawn transfer pipeline, by the emptying of residual liquid bacterial classification; By nutrition pipeline road, running water is sent into the strain cultivation tank of required cleaning, aseptic gas is sent in tank and cleaned simultaneously, after cleaning, by blowoff line, sewage is discharged;
B. sterilization: after cleaning, by nutrition pipeline road, high-temperature steam is sent in the strain cultivation tank of required sterilizing, carried out sterilizing;
(5) nutrition pipeline road, liquid spawn transfer pipeline cleaning and sterilizing: after each conveying finishes, need carry out cleaning and sterilizing to nutrition pipeline road, liquid spawn transfer pipeline;
A. nutrition pipeline road cleaning and sterilizing: running water is sent into nutrition pipeline road and clean; After cleaning finishes, high-temperature steam is sent into nutrition pipeline road and carry out disinfection;
B. liquid spawn transfer pipeline cleaning and sterilizing: running water is sent into liquid spawn transfer pipeline and clean; After cleaning finishes, high-temperature steam is sent into liquid spawn transfer pipeline and carry out disinfection.
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| CN107318626A (en) * | 2017-08-01 | 2017-11-07 | 深圳前海弘稼科技有限公司 | Nutrient solution cooling system |
| CN107493969A (en) * | 2017-09-01 | 2017-12-22 | 苏州顺泰元虫草生物科技有限公司 | A kind of high-efficient culture method of cordyceps sinensis |
| CN108076726B (en) * | 2017-12-28 | 2023-08-25 | 江苏哈工药机科技股份有限公司 | Sterile culture system for ginseng seeds |
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