CN103543139B - A kind of detection method of blood plasma low-abundance protein Surface enhanced raman spectroscopy - Google Patents
A kind of detection method of blood plasma low-abundance protein Surface enhanced raman spectroscopy Download PDFInfo
- Publication number
- CN103543139B CN103543139B CN201310520561.XA CN201310520561A CN103543139B CN 103543139 B CN103543139 B CN 103543139B CN 201310520561 A CN201310520561 A CN 201310520561A CN 103543139 B CN103543139 B CN 103543139B
- Authority
- CN
- China
- Prior art keywords
- low
- abundance protein
- blood plasma
- raman spectroscopy
- enhanced raman
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The present invention relates to a kind of method utilizing Surface enhanced raman spectroscopy to detect low-abundance protein in blood plasma.Getting plasma standard sample joins in the centrifugal column of hydrophobic polymer, is placed in shaking table concussion, centrifugally obtains low-abundance protein solution; With trace immobilization matrix, plasma standard sample mix, hatching and obtain rich plasma low-abundance protein trace immobilization matrix, add glacial acetic acid and add SERS active metal nanoparticles again, making low-abundance protein SERS enhancing matrix mixed solution through hatching; Get mixed liquor and carry out SERS detection, obtain the Surface enhanced raman spectroscopy data of blood plasma low-abundance protein.It is short that the present invention has detection time, and power demand is low, and immobilization matrix is cheap, and enrichment process is simple, has good economic and social benefit.
Description
Technical field
The present invention relates to protein analysis detection field, particularly one utilizes Surface enhanced raman spectroscopy (SERS) to detect the method for low-abundance protein in blood plasma.
Background technology
Raman spectrum is a kind of molecular vibration spectrum, is to obtain molecular vibration, rotation aspect information to the scattering spectrum analysis different from incident light frequency, and is applied to a kind of analytical approach of molecular structure research.Raman spectrum analysis process operation is simple and quick, highly sensitive, be a kind of Non-Destructive Testing, but raman spectral signal is faint, is subject to autofluorescence interference, therefore utilizes Raman spectroscopy directly to carry out detection and there is certain limitation.Surface enhanced raman spectroscopy (Surface-enhanced Raman spectroscopy, being called for short SERS) technology is a kind of conventional Raman signal Enhancement Method, when molecular adsorption is in some textured metal (as Au, Ag, Cu and Pt etc.) surface, internal field will be produced strengthen or Charger transfer, and make the Raman scattering intensities of these molecules increase by 10
4~ 10
14doubly, SERS effect that Here it is.SERS technology energy cancellation biomolecule fluorescence, the signal of acquisition is desirable, detects power demand lower, little, highly sensitive to biological tissue samples damage, can realize Single Molecule Detection.
Human plasma albumen contains the albumen deriving from nearly all cell, tissue, organ, but, the abundance difference of plasma proteins is huge, the albumen of 21 kinds of main high abundances and median abundance in blood plasma, as albumin, IgG, alpha1 Anti-trypsin, alpha2 Macroglobulin, transferrins etc. account for more than 99% of Total plasma protein content, remaining 1% is then made up of the even extremely low-abundance protein of numerous low abundance.When there is pathology in human tissue organ, albumen from pathological tissues or cell can enter into hematological system, pathology, the physiological situation of these protein and body are closely related, therefore, carrying out determination and analysis to these protein is find and one of most worthy sample of various disease association biomarker, these protein major parts are all low-abundance proteins, but, low-abundance protein is difficult to detect, and how effectively determination and analysis low-abundance protein is the challenging difficult problem in analysis science field always.Difficult point is mainly in two, and one is the detection limit of content lower than analytical instrument of low-abundance protein; On the other hand, in plasma proteins, the difference of high and low abundance protein content is huge, often can reach 10
12, the signal of low-abundance protein easily cover by high-abundance proteins.
At present following two kinds are mainly contained to the detection method of low-abundance protein: first method is first separated high and low abundance protein by two-dimensional gel electrophoresis, then carry out detection analysis with mass spectrum after low-abundance protein enzymolysis separation obtained; Second method is that the peptide section after chromatographic resolution protein digestion, finally carries out Mass Spectrometer Method analysis to peptide section by after first for high and low abundance protein mixed enzymolysis.It is large all to there is required sample size in said method, and consumptive material is expensive, and step is complicated, the problem such as longer consuming time.Mass spectrophotometry is subject to the interference of the factors such as eluent in addition, the quality protein such as fubaritic differentiation, and mass spectrometer is expensive, is difficult to extensive popularization.
Summary of the invention
The object of the invention is to propose a kind of method utilizing SERS technology for detection blood plasma low-abundance protein Surface enhanced raman spectroscopy.After the high-abundance proteins that this method adopts hydrophobic polymer to remove in blood plasma, utilize trace immobilization matrix enrichment low-abundance protein, utilize SERS technology for detection to obtain blood plasma low-abundance protein Surface enhanced raman spectroscopy.The present invention has the features such as quick, simple to operate, with low cost, the Surface enhanced raman spectroscopy of low-abundance protein in blood plasma effectively can be detected, thus overcome deficiency of the prior art.
For achieving the above object, the technical solution used in the present invention is as follows:
(1) blood plasma high-abundance proteins is removed
Getting hydrophobic polymer has in the collection tube of centrifugal column in cover, adds eluent, fully mixes, after the centrifugal 1 ~ 5min of 3000 ~ 6000rpm, outwelled by the liquid in collection tube, obtain the hydrophobic polymer through elution in triplicate.Get plasma standard sample, the eluent adding 1 ~ 10 times of volume dilutes, join containing in the centrifugal column of the hydrophobic polymer of elution, pipettor is utilized repeatedly to aspirate abundant mixing, be placed in shaking table 200 ~ 350rpm and shake 10 ~ 15min, 2000 ~ 5000rpm is centrifugal, removes sediment, collects the low-abundance protein solution on upper strata.
(2) rich plasma low-abundance protein
Get trace immobilization matrix, according to the ratio that trace immobilization Ji Zhi ︰ plasma standard sample is (1 ~ 2) g ︰ (10 ~ 20) μ l, the low-abundance protein solution collected with step (1) mixes, hatch 8 ~ 15min, then trace immobilization matrix proceeded in rinsing liquid and soak 10 ~ 20min, taking-up at room temperature dries up for subsequent use.
(3) low-abundance protein SERS strengthens the preparation of matrix mixed solution
The trace immobilization matrix that step (2) dries up is shredded and is collected in centrifuge tube, add glacial acetic acid to dissolve, abundant stirring is until when being rendered as transparent colloid state completely, add SERS active metal nanoparticles, abundant stirring, water-bath 42 ~ 65 DEG C hatches 15 ~ 30min, stratification, until solid phase impurity is separated out, make low-abundance protein SERS and strengthen matrix mixed solution.
(4) blood plasma low-abundance protein SERS spectral detection
Get low-abundance protein SERS enhancing matrix mixed solution 2 ~ 20 μ l and carry out SERS detection, obtain blood plasma low-abundance protein Surface enhanced raman spectroscopy data.
Described hydrophobic polymer refers to ion exchange resin or silica gel.
Described eluent refers to phosphate buffer, and the concentration range of phosphate radical is that 25 ~ 60 mMs often rise (mmol/L), and pH value is 4.
Described hydrophobic polymer and the amount ratio of eluent are the ratio of (2 ~ 25) g ︰ (100 ~ 1000) μ l,
Described trace immobilization matrix refers to cellulose acetate membrane, nitrocellulose filter, nylon membrane or PVDF membrane.
Described SERS active metal nanoparticles refers to Nano silver grain or golden nanometer particle.
The use amount of described hydrophobic polymer and the amount ratio of plasma standard sample are (2 ~ 25) g:(10 ~ 100) μ l.
Described trace immobilization matrix and the amount ratio of glacial acetic acid are (0.2 ~ 2) g ︰ (150 ~ 1000) μ l.
The amount ratio of described SERS active metal nanoparticles and trace immobilization matrix is (5 ~ 50) mM: (0.2 ~ 2) g.
Described rinsing liquid refers to the potpourri of glacial acetic acid, 95% ethanol, distilled water, and it consists of glacial acetic acid: 95% ethanol: the volume ratio of distilled water is (1 ~ 3): (4 ~ 9): (8 ~ 12);
Described SERS detects, and the laser power of use is 0.1 ~ 20mW, and laser excitation wavelength is 450 ~ 800nm, and the measurement range of spectrum is 400 ~ 3500cm
-1;
The preparation method of described Nano silver grain is:
Adopt oxammonium hydrochloride reduction preparation of silver nano particle, 4.5ml sodium hydroxide solution (0.1M) is mixed with 5ml hydroxylamine hydrochloride solution (0.06M), joins 90ml AgNO
3solution (1.1 × 10
-3m), in, vortex stirs 1min, and can obtain newborn grey Nano silver grain, particle diameter is approximately 40 ~ 60nm.By above-mentioned Nano silver grain with the centrifugal 10min ~ 15min of 4000 ~ 5000rpm, abandon supernatant, the concentrated Nano silver grain of lower floor then at room temperature lucifuge seal up for safekeeping for subsequent use.
The preparation method of described golden nanometer particle is:
Get the aqueous solution of chloraurate 500 milliliters containing 1mmol/L, be heated to the sodium citrate solution adding 50 milliliters of 38.8mmol/L after seething with excitement in rapid stirring.Continue heating 10 minutes, wait the color of solution after faint yellow change darkviolet, remove coverture agitating heating 15 minutes again, obtain golden nanometer particle.Particle diameter is approximately 35 ~ 45nm.By above-mentioned golden nanometer particle with the centrifugal 10min ~ 15min of 5000 ~ 8000rpm, supernatant is abandoned, the concentrated golden nanometer particle of lower floor then at room temperature lucifuge seal up for safekeeping for subsequent use.
Compared with prior art, the present invention has following beneficial effect:
1, the present invention adds glacial acetic acid when SERS active nano material mixes mutually with low-abundance protein, environment is made to keep faintly acid, because the SERS active nano materials itself such as silver or golden nanometer particle are electronegative, and the isoelectric point of blood plasma low abundance proteins is all at PH less than 7.5, under sour environment, blood plasma low abundance proteins protein is all positively charged, low-abundance protein sample to be measured like this strengthens stromal surface by electrostatic adsorbed close at SERS, and promote colloid aggregation, significantly improve detection sensitivity.The present invention uses SERS technology for detection low-abundance protein, and detection time only needs 1 ~ 10s, detects required laser power and is low to moderate 0.1mW, therefore, the present invention has fast, can't harm, sensitive detection advantage.
2, the present invention selects the immobilization matrix such as cellulose acetate film as the material of rich plasma low-abundance protein, material itself is cheap, enrichment process is simple, greatly reduce cost, and the SERS spectrum detecting the low-abundance protein obtained comprises rich in protein structural information, the deficiency of mass spectroscopy qualification low abundance proteins effectively can be made up.
Accompanying drawing explanation
Fig. 1 is the liver cancer patient blood plasma low-abundance protein Surface enhanced raman spectroscopy figure that the present invention records.
Fig. 2 is the nasopharyngeal carcinoma cancer patients blood plasma low-abundance protein Surface enhanced raman spectroscopy figure that the present invention records.
Fig. 3 is the Plasma of Patient With Gastric Cancer low-abundance protein Surface enhanced raman spectroscopy figure that the present invention records.
Embodiment
In Fig. 1, Fig. 2 and Fig. 3, ordinate is the intensity of spectral line, and the peak position of unit to be arbitrary unit (a.u) horizontal ordinate be each characteristic spectral line, with wave number (cm
-1) represent.
Embodiment 1
1, the liver cancer patient blood plasma low-abundance protein SERS spectral detection of substrate is strengthened based on Nano silver grain
(1) high-abundance proteins in liver cancer patient blood plasma is removed
Getting 2g ion exchange resin has in the collection tube of centrifugal column in cover, adds 100 μ l eluents, and mixing, after the centrifugal 5min of 3000rpm, outwells the liquid in collection tube, in triplicate.Get 10 μ l liver cancer patient plasma standard Sample Dilutions to mix in the eluent of 100 μ l, join in the centrifugal column containing ion exchange resin, pipettor is utilized repeatedly to aspirate abundant mixing, be placed in shaking table 200rpm and shake 15min, 2000rpm collected by centrifugation liver cancer patient blood plasma low-abundance protein solution.
(2) enrichment liver cancer patient blood plasma low-abundance protein
Get the liver cancer patient blood plasma low-abundance protein solution that 0.2g cellulose acetate film and step (1) collect and jointly hatch 15min, then cellulose acetate film is proceeded in rinsing liquid and soak 20min, take out at room temperature dry up for subsequent use.
(3) low-abundance protein SERS strengthens the preparation of matrix mixed solution
The cellulose acetate film that step (2) dries up is shredded and is collected in 1.5ml centrifuge tube, add glacial acetic acid 150 μ l and dissolve cellulose acetate film, abundant stirring is until when being rendered as transparent colloid state completely, add Nano silver grain, abundant stirring, water-bath 42 DEG C hatches 30min, stratification, until solid phase impurity is separated out, make low-abundance protein SERS and strengthen matrix mixed solution
(4) liver cancer patient blood plasma low-abundance protein SERS spectral detection
Get liver cancer patient blood plasma low-abundance protein SERS enhancing matrix mixed solution 2 μ l and carry out SERS detection.The SERS spectrum of liver cancer patient blood plasma low-abundance protein detected as shown in Figure 1.
Embodiment 2
The plasma of patients with nasopharyngeal carcinoma low-abundance protein SERS spectral detection of substrate is strengthened based on golden nanometer particle
(1) high-abundance proteins in plasma of patients with nasopharyngeal carcinoma is removed
Getting 25g ion exchange resin has in the collection tube of centrifugal column in cover, adds 1000 μ l eluents and hydrophobic polymer fully mixes, after the centrifugal 1min of 6000rpm, is outwelled by the liquid in collection tube, in triplicate.Get 100 μ l plasma of patients with nasopharyngeal carcinoma standard models to be diluted in the eluent of 1000 μ l and to mix, join in the centrifugal column containing ion exchange resin, pipettor is utilized repeatedly to aspirate abundant mixing, be placed in shaking table 350rpm and shake 10min, 5000rpm collected by centrifugation plasma of patients with nasopharyngeal carcinoma low-abundance protein solution.
(2) enrichment plasma of patients with nasopharyngeal carcinoma low-abundance protein
Get the plasma of patients with nasopharyngeal carcinoma low-abundance protein solution that 2g PVDF membrane and step (2) collect and jointly hatch 8min, then PVDF membrane is proceeded in rinsing liquid and soak 10min, take out at room temperature dry up for subsequent use.
(3) plasma of patients with nasopharyngeal carcinoma low-abundance protein SERS spectral detection
The PVDF membrane that step (2) dries up is shredded and is collected in 7ml centrifuge tube, add glacial acetic acid 1000 μ l and dissolve PVDF membrane, abundant stirring is until when being rendered as transparent colloid state completely, add golden nanometer particle, abundant stirring, water-bath 65 DEG C hatches 15min, stratification, until solid phase impurity is separated out, make low-abundance protein SERS and strengthen matrix mixed solution.
(4) plasma of patients with nasopharyngeal carcinoma low-abundance protein SERS spectral detection
Get plasma of patients with nasopharyngeal carcinoma low-abundance protein SERS enhancing matrix mixed solution 20 μ l and carry out SERS detection.The SERS spectrum of plasma of patients with nasopharyngeal carcinoma low-abundance protein detected as shown in Figure 2.
Embodiment 3
1, the Plasma of Patient With Gastric Cancer low-abundance protein SERS spectral detection of substrate is strengthened based on Nano silver grain
(1) high-abundance proteins in Plasma of Patient With Gastric Cancer is removed
Getting 2g silica gel has in the collection tube of centrifugal column in cover, adds 150 μ l eluents, and mixing, after the centrifugal 5min of 3000rpm, outwells the liquid in collection tube, in triplicate.Get 10 μ l Plasma of Patient With Gastric Cancer standard models to be diluted in the eluent of 100 μ l and to mix, join in the centrifugal column containing silica gel, utilize pipettor repeatedly to aspirate abundant mixing, be placed in shaking table 200rpm and shake 15min, 2000rpm collected by centrifugation liver cancer patient blood plasma low-abundance protein solution.
(2) enrichment Plasma of Patient With Gastric Cancer low-abundance protein
Get the Plasma of Patient With Gastric Cancer low-abundance protein solution that 2g nitrocellulose filter and step (1) collect and jointly hatch 15min, then nitrocellulose membrane is proceeded in rinsing liquid and soak 20min, take out at room temperature dry up for subsequent use.
(3) low-abundance protein SERS strengthens the preparation of matrix mixed solution
The nitrocellulose membrane that step (2) dries up is shredded and is collected in 1.5ml centrifuge tube, add glacial acetic acid 1000 μ l and dissolve nitrocellulose membrane, abundant stirring is until when being rendered as transparent colloid state completely, add Nano silver grain, abundant stirring, water-bath 52 DEG C hatches 18min, stratification, until solid phase impurity is separated out, make low-abundance protein SERS and strengthen matrix mixed solution.
(4) blood plasma low-abundance protein SERS spectral detection
Get Plasma of Patient With Gastric Cancer low-abundance protein SERS enhancing matrix mixed solution 2 μ l and carry out SERS detection.The SERS spectrum of Plasma of Patient With Gastric Cancer low-abundance protein detected as shown in Figure 3.
Certainly, the process of the present embodiment is only for illustration of technical scheme of the present invention, those skilled in the art are according to enlightenment of the present invention, also can be easy to expect the multiple low-abundance protein of other except blood plasma low-abundance protein, as organized low-abundance protein, saliva low-abundance protein, seminal fluid low-abundance protein and urine low-abundance protein etc., detect in a similar manner.But all those skilled in the art are because of technology involved in the present invention enlightenment, and the technical scheme adopting equivalent replacement or equivalent deformation mode to be formed all drops in protection scope of the present invention.
Claims (11)
1. a detection method for blood plasma low-abundance protein Surface enhanced raman spectroscopy, is characterized in that:
(1) blood plasma high-abundance proteins is removed
Getting hydrophobic polymer has in the collection tube of centrifugal column in cover, adds eluent, fully mixes, after the centrifugal 1 ~ 5min of 3000 ~ 6000rpm, outwelled by the liquid in collection tube, obtain the hydrophobic polymer through elution in triplicate;
Get plasma standard sample, the eluent adding 1 ~ 10 times of volume dilutes, join containing in the centrifugal column of the hydrophobic polymer of elution, pipettor is utilized repeatedly to aspirate abundant mixing, be placed in shaking table 200 ~ 350rpm and shake 10 ~ 15min, 2000 ~ 5000rpm is centrifugal, removes sediment, collects the low-abundance protein solution on upper strata;
(2) rich plasma low-abundance protein
Get trace immobilization matrix, the low-abundance protein solution collected with step (1) mixes, and hatches 8 ~ 15min, then trace immobilization matrix is proceeded in rinsing liquid and soaks 10 ~ 20min, and taking-up at room temperature dries up for subsequent use;
(3) low-abundance protein SERS strengthens the preparation of matrix mixed solution
The trace immobilization matrix that step (2) dries up is shredded and is collected in centrifuge tube, add glacial acetic acid to dissolve, abundant stirring is until when being rendered as transparent colloid state completely, add SERS active metal nanoparticles, abundant stirring, water-bath 42 ~ 65 DEG C hatches 15 ~ 30min, stratification, until solid phase impurity is separated out, make low-abundance protein SERS and strengthen matrix mixed solution;
(4) blood plasma low-abundance protein SERS spectral detection
Get low-abundance protein SERS enhancing matrix mixed solution 2 ~ 20 μ l and carry out SERS detection, obtain blood plasma low-abundance protein Surface enhanced raman spectroscopy data.
2. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, when it is characterized in that described trace immobilization matrix and plasma standard sample mix, its ratio is (0.2 ~ 2) g ︰ (10 ~ 100) μ l.
3. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, is characterized in that described hydrophobic polymer refers to ion exchange resin or silica gel.
4. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, it is characterized in that described eluent refers to phosphate buffer, the concentration range of phosphate radical is that 25 ~ 60 mMs often rise, and pH value is 4.
5. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, is characterized in that the amount ratio of described hydrophobic polymer and eluent is (2 ~ 25) g ︰ (100 ~ 1000) μ l.
6. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, is characterized in that described trace immobilization matrix refers to cellulose acetate membrane, nitrocellulose filter, nylon membrane or PVDF membrane.
7. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, is characterized in that described SERS active metal nanoparticles refers to Nano silver grain or golden nanometer particle.
8. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, is characterized in that the use amount of described hydrophobic polymer and the amount ratio of plasma standard sample are (2 ~ 25) g:(10 ~ 100) μ l.
9. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, is characterized in that the amount ratio of described trace immobilization matrix and glacial acetic acid is (0.2 ~ 2) g ︰ (150 ~ 1000) μ l.
10. the detection method of described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, is characterized in that the amount ratio of described SERS active metal nanoparticles and trace immobilization matrix is (5 ~ 50) mM: (0.2 ~ 2) g.
The detection method of 11. described blood plasma low-abundance protein Surface enhanced raman spectroscopy according to claim 1, it is characterized in that described SERS detects, the laser power used is 0.1 ~ 20mW, and laser excitation wavelength is 450 ~ 800nm, and the measurement range of spectrum is 400 ~ 3500cm
-1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310520561.XA CN103543139B (en) | 2013-10-29 | 2013-10-29 | A kind of detection method of blood plasma low-abundance protein Surface enhanced raman spectroscopy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310520561.XA CN103543139B (en) | 2013-10-29 | 2013-10-29 | A kind of detection method of blood plasma low-abundance protein Surface enhanced raman spectroscopy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103543139A CN103543139A (en) | 2014-01-29 |
| CN103543139B true CN103543139B (en) | 2015-10-14 |
Family
ID=49966806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310520561.XA Active CN103543139B (en) | 2013-10-29 | 2013-10-29 | A kind of detection method of blood plasma low-abundance protein Surface enhanced raman spectroscopy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103543139B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122247B (en) * | 2014-06-10 | 2017-01-25 | 南京大学 | Glycoprotein detection method based on molecular imprinting technique and Raman spectrum and application |
| CN106436027B (en) * | 2016-09-09 | 2018-12-21 | 中国科学院合肥物质科学研究院 | Silver nanoparticle square-cellulose acetate complex microsphere film and its preparation method and application |
| GB201704128D0 (en) | 2017-03-15 | 2017-04-26 | Univ Swansea | Method and apparatus for use in diagnosis and monitoring of colorectal cancer |
| CN107132209A (en) * | 2017-04-28 | 2017-09-05 | 南京理工大学 | A kind of method of the label-free detection bovine serum albumin of Raman enhancing substrate based on Nano Silver/graphene oxide/sodium chloride |
| CN110646401B (en) * | 2019-10-12 | 2022-04-12 | 福建师范大学 | SERS detection method based on hydroxyapatite nanoparticle adsorbed protein |
| CN112331270B (en) * | 2021-01-04 | 2021-03-23 | 中国工程物理研究院激光聚变研究中心 | Construction method of novel coronavirus Raman spectrum data center |
| CN114034641A (en) * | 2021-12-08 | 2022-02-11 | 重庆大学 | Narrow-band filter-based Raman on-chip detection system and method |
| CN114705794B (en) * | 2022-04-15 | 2022-12-02 | 西湖大学 | Proteomics analysis method for biological sample |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1811407A (en) * | 2006-01-26 | 2006-08-02 | 复旦大学 | Method for one-step desalting and enriching on low-abundance protein target |
| CN1902495A (en) * | 2003-12-30 | 2007-01-24 | 英特尔公司 | Methods for using Raman spectroscopy to obtain a protein profile of a biological sample |
-
2013
- 2013-10-29 CN CN201310520561.XA patent/CN103543139B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1902495A (en) * | 2003-12-30 | 2007-01-24 | 英特尔公司 | Methods for using Raman spectroscopy to obtain a protein profile of a biological sample |
| CN1811407A (en) * | 2006-01-26 | 2006-08-02 | 复旦大学 | Method for one-step desalting and enriching on low-abundance protein target |
Non-Patent Citations (3)
| Title |
|---|
| 人血浆低丰度蛋白富集方法的研究进展;张雪群等;《中华检验医学杂志》;20061031;第29卷(第10期);第947-950页 * |
| 冯尚源等.基于SERS技术结合多变量统计分析胃癌患者血浆拉曼光谱.《中国科学:生命科学》.2011,第41卷(第7期),第550-557页. * |
| 硅胶修饰-表面分子印迹牛血红蛋白及其识别性能的研究;刘秋叶等;《化学学报》;20081231;第66卷(第1期);第56-62页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103543139A (en) | 2014-01-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103543139B (en) | A kind of detection method of blood plasma low-abundance protein Surface enhanced raman spectroscopy | |
| CN101799421B (en) | Detection method of body fluid surface enhanced Raman spectroscopy (SERS) | |
| Pauli et al. | Lab-in-a-syringe using gold nanoparticles for rapid immunosensing of protein biomarkers | |
| Bonnier et al. | Screening the low molecular weight fraction of human serum using ATR‐IR spectroscopy | |
| CN102095717B (en) | Method for detecting BHA (butylated hydroxyanisole) in edible oil and plastic packages by laser nanometer Raman spectroscopy | |
| Locatelli et al. | Biofluid sampler: A new gateway for mail-in-analysis of whole blood samples | |
| CN111650370B (en) | Method and device for detecting novel coronavirus SARS-CoV-2 | |
| CN101046473A (en) | Method of improving stability of antigen or antibody particle combined with latex | |
| CN102175664A (en) | Method for detecting surface enhanced Raman spectra of blood RNA | |
| CN1808117A (en) | Method for detecting citrinin content in red koji fermentation product | |
| CN103257134A (en) | Method for preparing surface-enhanced Raman scattering (SERS) substrate based on capillary tube | |
| Liang et al. | Filter-membrane-based ultrafiltration coupled with surface-enhanced raman spectroscopy for potential differentiation of benign and malignant thyroid tumors from blood plasma | |
| CN110646401A (en) | SERS detection method based on hydroxyapatite nanoparticle adsorbed protein | |
| CN103837516A (en) | Method for rapidly detecting glucose concentration based on gold nanocluster fluorescent probe | |
| Yang et al. | Simultaneous determination of four phenolic acids in traditional Chinese medicine by capillary electrophoresis-chemiluminescence | |
| CN105158225B (en) | A kind of method of two-photon fluorescence excitation detection melamine | |
| Nair et al. | Non enzymatic colorimetric detection of glucose using cyanophenyl boronic acid included β-cyclodextrin stabilized gold nanoparticles | |
| CN106706588A (en) | Heterogeneous photocatalysis resonance fluorescence method for accurately detecting trace uranium in environmental water sample | |
| CN110806441B (en) | Detection method for metal ions in dendrobium officinale | |
| Zhang et al. | A field amplification enhanced paper-based analytical device with a robust chemiluminescence detection module | |
| Zhang et al. | Highly sensitive determination of nitric oxide in biologic samples by a near-infrared BODIPY-based fluorescent probe coupled with high-performance liquid chromatography | |
| Hamer et al. | Development of an electrophoretic method based on nanostructured materials for HbA1c determination | |
| Castro et al. | Exploring alternative ovarian cancer biomarkers using innovative nanotechnology strategies | |
| CN106124477A (en) | A kind of nanometer silver course of dissolution discharges concentration of silver ions and the detection method of speed | |
| CN101329346A (en) | Optimizing mass spectrogram model for detecting breast cancer characteristic protein and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |